GEM1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following GEM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GEM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi00831 NM_001178193.1
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Saccharomyces cerevisiae S288C ERMES complex Ca(2+)-binding regulatory GTPase GEM1 (GEM1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSi00831
    Clone ID Related Accession (Same CDS sequence) NM_001178193.1
    Accession Version NM_001178193.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1989bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product ERMES complex Ca(2+)-binding regulatory GTPase GEM1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001133). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGACTAAAG AAACGATTCG GGTAGTTATT TGCGGTGATG AAGGGGTTGG TAAATCCAGT 
    CTGATTGTAT CATTAACAAA AGCTGAATTC ATACCGACCA TACAGGACGT GCTGCCACCC
    ATCAGTATCC CAAGAGATTT CTCATCATCA CCTACATATT CTCCTAAGAA TACAGTACTT
    ATAGACACTT CAGATTCGGA CCTCATAGCT TTAGACCATG AGTTGAAGTC CGCCGACGTA
    ATTTGGCTTG TGTATTGCGA TCACGAATCG TATGACCATG TTTCTCTCTT TTGGTTGCCT
    CATTTCAGAT CCCTGGGGTT GAATATTCCT GTCATTCTCT GCAAAAATAA ATGTGATTCC
    ATATCGAATG TTAATGCCAA TGCAATGGTC GTGTCAGAGA ACAGTGATGA TGATATCGAT
    ACCAAAGTGG AGGATGAAGA ATTTATCCCA ATATTAATGG AGTTTAAAGA AATCGACACT
    TGCATTAAGA CAAGTGCCAA GACACAGTTT GATCTTAACC AAGCGTTTTA TCTTTGCCAA
    AGGGCTATAA CACACCCAAT ATCACCTTTA TTTGATGCCA TGGTAGGTGA ACTAAAGCCA
    TTGGCTGTCA TGGCTTTAAA AAGAATTTTT CTTTTAAGCG ATTTAAACCA GGACTCATAT
    TTAGATGACA ACGAAATCTT GGGCTTACAA AAAAAGTGCT TCAATAAGAG TATCGATGTA
    AACGAACTGA ATTTTATTAA AGATTTGCTT TTGGATATTT CCAAGCACGA TCAAGAGTAC
    ATTAACCGCA AGCTATACGT ACCGGGAAAA GGCATCACCA AAGATGGTTT CCTTGTACTG
    AACAAAATAT ACGCTGAAAG GGGGAGACAC GAAACTACAT GGGCTATCCT AAGAACTTTC
    CATTACACAG ATTCCTTGTG TATTAATGAC AAAATTCTCC ATCCGAGGTT GGTTGTCCCT
    GACACTTCCA GTGTGGAATT GAGCCCCAAG GGCTACAGAT TTCTTGTAGA TATTTTTTTG
    AAGTTTGATA TCGACAATGA TGGTGGTTTG AATAATCAAG AATTACATCG TCTATTTAAG
    TGCACACCAG GGCTGCCTAA ACTATGGACC TCAACGAATT TCCCCTTCTC CACTGTCGTA
    AACAACAAGG GTTGCATCAC CTTACAAGGC TGGCTAGCAC AATGGAGTAT GACGACTTTC
    TTGAACTATA GCACAACTAC CGCTTACTTG GTGTATTTTG GCTTTCAGGA AGATGCAAGA
    CTAGCCCTAC AAGTAACCAA GCCAAGGAAA ATGAGACGCC GTTCCGGGAA ACTTTACAGA
    TCCAATATCA ATGACAGAAA AGTGTTCAAT TGCTTTGTCA TTGGAAAGCC ATGTTGCGGC
    AAAAGCTCTT TGCTAGAGGC CTTCTTGGGC AGATCTTTCT CGGAGGAGTA TTCTCCGACA
    ATCAAACCAA GAATTGCAGT CAATAGTTTA GAACTCAAAG GTGGGAAACA GTACTATTTG
    ATTTTGCAAG AACTTGGAGA ACAGGAATAC GCCATACTAG AGAATAAGGA TAAGTTAAAA
    GAATGTGACG TAATCTGTCT AACATACGAT TCCAGCGACC CAGAATCATT CTCCTACTTA
    GTTTCGCTTC TAGACAAATT CACACATTTA CAAGATCTAC CGTTGGTATT TGTAGCTTCT
    AAGGCAGATT TGGATAAGCA GCAACAAAGG TGTCAAATCC AACCAGATGA ACTGGCAGAC
    GAGCTATTTG TGAACCACCC ACTGCACATA TCATCCAGAT GGCTAAGTTC CCTTAATGAA
    TTGTTTATTA AAATCACAGA AGCTGCTCTT GATCCTGGCA AAAACACTCC GGGATTGCCA
    GAAGAAACAG CAGCAAAAGA TGTCGACTAC AGACAAACGG CTCTCATTTT TGGGTCCACT
    GTTGGATTCG TAGCACTATG TTCCTTTACG TTAATGAAAT TATTCAAATC ATCAAAATTC
    TCAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_009351.1
    CDS1..1989
    Translation

    Target ORF information:

    RefSeq Version NM_001178193.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C ERMES complex Ca(2+)-binding regulatory GTPase GEM1 (GEM1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001178193.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGACTAAAG AAACGATTCG GGTAGTTATT TGCGGTGATG AAGGGGTTGG TAAATCCAGT 
    CTGATTGTAT CATTAACAAA AGCTGAATTC ATACCGACCA TACAGGACGT GCTGCCACCC
    ATCAGTATCC CAAGAGATTT CTCATCATCA CCTACATATT CTCCTAAGAA TACAGTACTT
    ATAGACACTT CAGATTCGGA CCTCATAGCT TTAGACCATG AGTTGAAGTC CGCCGACGTA
    ATTTGGCTTG TGTATTGCGA TCACGAATCG TATGACCATG TTTCTCTCTT TTGGTTGCCT
    CATTTCAGAT CCCTGGGGTT GAATATTCCT GTCATTCTCT GCAAAAATAA ATGTGATTCC
    ATATCGAATG TTAATGCCAA TGCAATGGTC GTGTCAGAGA ACAGTGATGA TGATATCGAT
    ACCAAAGTGG AGGATGAAGA ATTTATCCCA ATATTAATGG AGTTTAAAGA AATCGACACT
    TGCATTAAGA CAAGTGCCAA GACACAGTTT GATCTTAACC AAGCGTTTTA TCTTTGCCAA
    AGGGCTATAA CACACCCAAT ATCACCTTTA TTTGATGCCA TGGTAGGTGA ACTAAAGCCA
    TTGGCTGTCA TGGCTTTAAA AAGAATTTTT CTTTTAAGCG ATTTAAACCA GGACTCATAT
    TTAGATGACA ACGAAATCTT GGGCTTACAA AAAAAGTGCT TCAATAAGAG TATCGATGTA
    AACGAACTGA ATTTTATTAA AGATTTGCTT TTGGATATTT CCAAGCACGA TCAAGAGTAC
    ATTAACCGCA AGCTATACGT ACCGGGAAAA GGCATCACCA AAGATGGTTT CCTTGTACTG
    AACAAAATAT ACGCTGAAAG GGGGAGACAC GAAACTACAT GGGCTATCCT AAGAACTTTC
    CATTACACAG ATTCCTTGTG TATTAATGAC AAAATTCTCC ATCCGAGGTT GGTTGTCCCT
    GACACTTCCA GTGTGGAATT GAGCCCCAAG GGCTACAGAT TTCTTGTAGA TATTTTTTTG
    AAGTTTGATA TCGACAATGA TGGTGGTTTG AATAATCAAG AATTACATCG TCTATTTAAG
    TGCACACCAG GGCTGCCTAA ACTATGGACC TCAACGAATT TCCCCTTCTC CACTGTCGTA
    AACAACAAGG GTTGCATCAC CTTACAAGGC TGGCTAGCAC AATGGAGTAT GACGACTTTC
    TTGAACTATA GCACAACTAC CGCTTACTTG GTGTATTTTG GCTTTCAGGA AGATGCAAGA
    CTAGCCCTAC AAGTAACCAA GCCAAGGAAA ATGAGACGCC GTTCCGGGAA ACTTTACAGA
    TCCAATATCA ATGACAGAAA AGTGTTCAAT TGCTTTGTCA TTGGAAAGCC ATGTTGCGGC
    AAAAGCTCTT TGCTAGAGGC CTTCTTGGGC AGATCTTTCT CGGAGGAGTA TTCTCCGACA
    ATCAAACCAA GAATTGCAGT CAATAGTTTA GAACTCAAAG GTGGGAAACA GTACTATTTG
    ATTTTGCAAG AACTTGGAGA ACAGGAATAC GCCATACTAG AGAATAAGGA TAAGTTAAAA
    GAATGTGACG TAATCTGTCT AACATACGAT TCCAGCGACC CAGAATCATT CTCCTACTTA
    GTTTCGCTTC TAGACAAATT CACACATTTA CAAGATCTAC CGTTGGTATT TGTAGCTTCT
    AAGGCAGATT TGGATAAGCA GCAACAAAGG TGTCAAATCC AACCAGATGA ACTGGCAGAC
    GAGCTATTTG TGAACCACCC ACTGCACATA TCATCCAGAT GGCTAAGTTC CCTTAATGAA
    TTGTTTATTA AAATCACAGA AGCTGCTCTT GATCCTGGCA AAAACACTCC GGGATTGCCA
    GAAGAAACAG CAGCAAAAGA TGTCGACTAC AGACAAACGG CTCTCATTTT TGGGTCCACT
    GTTGGATTCG TAGCACTATG TTCCTTTACG TTAATGAAAT TATTCAAATC ATCAAAATTC
    TCAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


    The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.
    Proceedings of the National Academy of Sciences of the United States of America92(9)3809-13(1995 Apr)
    Bussey H,Kaback DB,Zhong W,Vo DT,Clark MW,Fortin N,Hall J,Ouellette BF,Keng T,Barton AB