Rhot2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rhot2
Entrez Gene ID	287156
Full Name	ras homolog family member T2
Synonyms	Miro2
General protein information	Preferred Names ras homolog family member T2 Names mitochondrial Rho GTPase 2 MIRO-2 ras homolog gene family, member T2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q12

mRNA and Protein(s)

mRNA	Protein	Name
XM_017597069.1	XP_017452558.1	mitochondrial Rho GTPase 2 isoform X1
NM_181823.1	NP_861544.1	mitochondrial Rho GTPase 2

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ADR402W	NP_984497.1
Neurospora crassa	NCU03966	XP_957605.1
Oryza sativa (rice)	Os01g0843300	NP_001044775.1
Pan troglodytes (chimpanzee)	RHOT2	XP_510715.4
Homo sapiens (human)	RHOT2	NP_620124.1
Mus musculus (house mouse)	Rhot2	NP_666111.1
Danio rerio (zebrafish)	rhot2	NP_001032768.1
Kluyveromyces lactis	KLLA0A03465g	XP_451152.1
Magnaporthe oryzae (rice blast fungus)	MGG_01044	XP_003717898.1
Arabidopsis thaliana (thale cress)	MIRO1	NP_198106.1
Oryza sativa (rice)	Os03g0810600	NP_001051665.1
Saccharomyces cerevisiae (baker's yeast)	GEM1	NP_009351.1
	RHOT2	XP_001118510.2
Rattus norvegicus (Norway rat)	Rhot2	NP_861544.1
Canis lupus familiaris (dog)	RHOT2	XP_005622161.1
Bos taurus (cattle)	RHOT2	NP_847886.3
Gallus gallus (chicken)	RHOT2	NP_001074335.1
Schizosaccharomyces pombe (fission yeast)	SPCC320.04c	NP_587725.1
Xenopus tropicalis (tropical clawed frog)	rhot2	NP_001006725.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of mitochondrial transport in neurons.
Lin MY, Sheng ZH
Experimental cell research334(1)35-44(2015 May)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

HUMMR, a hypoxia- and HIF-1alpha-inducible protein, alters mitochondrial distribution and transport.
Li Y, Lim S, Hoffman D, Aspenstrom P, Federoff HJ, Rempe DA
The Journal of cell biology185(6)1065-81(2009 Jun)

Bidirectional Ca2+-dependent control of mitochondrial dynamics by the Miro GTPase.
Saotome M, Safiulina D, Szabadkai G, Das S, Fransson A, Aspenstrom P, Rizzuto R, Hajn?czky G
Proceedings of the National Academy of Sciences of the United States of America105(52)20728-33(2008 Dec)

The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in mitochondrial trafficking.
Fransson S, Ruusala A, Aspenstr?m P
Biochemical and biophysical research communications344(2)500-10(2006 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Miro proteins serve as a [Ca(2+)](c)-sensitive switch and bifunctional regulator for both the motility and fusion-fission dynamics of the mitochondria.
Title: Bidirectional Ca2+-dependent control of mitochondrial dynamics by the Miro GTPase.

The following Rhot2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rhot2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13076	NM_181823.1 Latest version!	Rattus norvegicus ras homolog family member T2 (Rhot2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa13076	XM_017597069.1 Latest version!	Rattus norvegicus ras homolog family member T2 (Rhot2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rhot2 ( NM_181823.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_861544.1 Rattus norvegicus ras homolog family member T2 (Rhot2), mRNA.

Price & Availability↑

CloneID	ORa13076
Clone ID Related Accession (Same CDS sequence)	XM_017597069.1 , NM_181823.1
Accession Version	NM_181823.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-14
Organism	Rattus norvegicus(Norway rat)
Product	mitochondrial Rho GTPase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY303973.1. ##Evidence-Data-START## Transcript exon combination :: AY303973.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGAGGCGCG ACGTGCGCAT CCTGCTGCTG GGCGAGGCCC AGGTGGGGAA GACTTCTCTG 
ATCCTGTCGC TGGTCGGCGA GGAGTTTCCC GAGGAGGTCC CTGCCCGCGC GGAGGAGATA 
ACCATCCCCG CCGACGTCAC CCCAGAGAAG GTGCCCACCC ATATCGTGGA TTACTCAGAA 
GCGGAGCAGA CAGAGGAGGA ACTTCAGGAG GAGATCCACA AGGCAAACGT GGTGTGTGTG 
GTGTACGATG TGTCTGAGGA AGCCACCATT GAGAAGATCC GAACCAAGTG GATTCCCCTC 
GTGAATGGCA GGACGGCAAC AGGGCCCAGG TTGCCCATCA TCCTGGTAGG CAATAAGTCA 
GACCTGCGGC CAGGGAGTAC CATAGAGGCT GTGTTGCCCA TTATGAGCCA GTTTCCTGAG 
ATAGAGACCT GTGTGGAGTG TTCTGCCAAG CACTTGAGGA ATATCTCAGA GCTGTTCTAC 
TATGCCCAGA AGGCTGTTCT GCATCCCACC GCTCCCCTTT ATGACCCTGA GGCCAAACAG 
TTAAGGCCTG CCTGCGCCCA GGCACTCACA CGCATCTTCA GGCTGTCGGA TCAGGACCTA 
GACCACGCAC TCAGCGACAA GGAGCTCAAT GCCTTCCAGA AGTCCTGCTT TGGCCATCCT 
CTGGCCCCAC AAGCCCTGGA GGATGTAAAG AGGGTAGTGT GCAAGAATGT GGCAGGTGGT 
GTGCAGGATG ACCGACTGAC CCTGGAAGGC TTCCTCTTCC TGAACACACT TTTCATCCAG 
CGTGGCCGGC ATGAGACTAC GTGGACCATC CTACGGCGCT TTGGTTACAG TGACTCGCTG 
GAGCTGACTC CTGACTACCT CTGTCCACCG CTCTACGTAC CCCCTGGCTG CAGCACCGAG 
CTCAACCATC GTGGCTACCA GTTTGTGCAG CGGGTGTTTG AGAAGCATGA TCAGGACCAT 
GATGGTGTCC TCTCACCCAC GGAACTAGAG AGTCTCTTCA GCGTGTTCTC AGTTGCTCCG 
TGGGGCCCTG AACTCTTACA CACAGTCCCC ACTGAGGCTG GCTGTCTGTC CTTGCGTGGA 
TATCTCTGCC AGTGGACCCT GGTGACCTAC TTGGATGTCC AGCACTGCCT TGCCCATCTT 
GGCTACCTGG GTTACCCTAC CCTCTGTGAG CAGGACTCAC AGGCACAGGC TATCACAGTT 
ACCCGCGAGA AGAGACTGGA CCAGGAGAAA GGGCAAACAC AGCGCAGCGT CCTCATGTGT 
AAGGTGTTGG GAGCCCGAGG AGTGGGCAAG TCGGCCTTCT TGCAGGCCTT CCTCGGCCAC 
AGCCTTGGGG AAGCCAGGGA CAGGGACGCC CCTGAGAAAT TGCCCACGCA TACCATCAAT 
ACAGTGCGGG TCAGCGGACA GGAAAAGTAC CTGATTCTGT GTGAAGTGAA TGCTGACAGC 
CTGCTGGACA CCTCTCTAGA CACTACCTGT GATGTCGCCT GCTTAATGTT TGACAGCAGT 
GACCCCGAGA CCTTCGTGCA GTGTGCTACC ATCTACAAGC GTTATTACAT GGATGGGCAG 
ACACCCTGCC TCTTTATCTC CTCCAAAGCT GATCTCCCTG AAGGTGTTGC CCCACCAGGC 
CTATCACCAG CTGAGTTCTG CCGTAGGCAC CGGCTGCCAG CCCCGGCCTC ATTCTCCTGC 
TTGGGACCAG CACAGTCCAG CATAGATGTC TTTACCCAAC TTGCTACTAT GGCCACTTTC 
CCACACCTGG CGCACACAGA GCTGCACCCC ACCCCCTTCT GGCTCCGAGG CGTGCTGGTG 
GCTGTTGGGA CCGCGGTGGC TGCTGTCCTT AGCTTCTCAC TATATAGGGT CCTAGTGAAG 
AGCCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_861544.1
CDS	368..2236
Translation	MRRDVRILLLGEAQVGKTSLILSLVGEEFPEEVPARAEEITIPADVTPEKVPTHIVDYSEAEQTEEELQEEIHKANVVCVVYDVSEEATIEKIRTKWIPLVNGRTATGPRLPIILVGNKSDLRPGSTIEAVLPIMSQFPEIETCVECSAKHLRNISELFYYAQKAVLHPTAPLYDPEAKQLRPACAQALTRIFRLSDQDLDHALSDKELNAFQKSCFGHPLAPQALEDVKRVVCKNVAGGVQDDRLTLEGFLFLNTLFIQRGRHETTWTILRRFGYSDSLELTPDYLCPPLYVPPGCSTELNHRGYQFVQRVFEKHDQDHDGVLSPTELESLFSVFSVAPWGPELLHTVPTEAGCLSLRGYLCQWTLVTYLDVQHCLAHLGYLGYPTLCEQDSQAQAITVTREKRLDQEKGQTQRSVLMCKVLGARGVGKSAFLQAFLGHSLGEARDRDAPEKLPTHTINTVRVSGQEKYLILCEVNADSLLDTSLDTTCDVACLMFDSSDPETFVQCATIYKRYYMDGQTPCLFISSKADLPEGVAPPGLSPAEFCRRHRLPAPASFSCLGPAQSSIDVFTQLATMATFPHLAHTELHPTPFWLRGVLVAVGTAVAAVLSFSLYRVLVKSR

Target ORF information:

RefSeq Version	NM_181823.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ras homolog family member T2 (Rhot2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181823.1

ORF Insert Sequence:

ATGAGGCGCG ACGTGCGCAT CCTGCTGCTG GGCGAGGCCC AGGTGGGGAA GACTTCTCTG 
ATCCTGTCGC TGGTCGGCGA GGAGTTTCCC GAGGAGGTCC CTGCCCGCGC GGAGGAGATA 
ACCATCCCCG CCGACGTCAC CCCAGAGAAG GTGCCCACCC ATATCGTGGA TTACTCAGAA 
GCGGAGCAGA CAGAGGAGGA ACTTCAGGAG GAGATCCACA AGGCAAACGT GGTGTGTGTG 
GTGTACGATG TGTCTGAGGA AGCCACCATT GAGAAGATCC GAACCAAGTG GATTCCCCTC 
GTGAATGGCA GGACGGCAAC AGGGCCCAGG TTGCCCATCA TCCTGGTAGG CAATAAGTCA 
GACCTGCGGC CAGGGAGTAC CATAGAGGCT GTGTTGCCCA TTATGAGCCA GTTTCCTGAG 
ATAGAGACCT GTGTGGAGTG TTCTGCCAAG CACTTGAGGA ATATCTCAGA GCTGTTCTAC 
TATGCCCAGA AGGCTGTTCT GCATCCCACC GCTCCCCTTT ATGACCCTGA GGCCAAACAG 
TTAAGGCCTG CCTGCGCCCA GGCACTCACA CGCATCTTCA GGCTGTCGGA TCAGGACCTA 
GACCACGCAC TCAGCGACAA GGAGCTCAAT GCCTTCCAGA AGTCCTGCTT TGGCCATCCT 
CTGGCCCCAC AAGCCCTGGA GGATGTAAAG AGGGTAGTGT GCAAGAATGT GGCAGGTGGT 
GTGCAGGATG ACCGACTGAC CCTGGAAGGC TTCCTCTTCC TGAACACACT TTTCATCCAG 
CGTGGCCGGC ATGAGACTAC GTGGACCATC CTACGGCGCT TTGGTTACAG TGACTCGCTG 
GAGCTGACTC CTGACTACCT CTGTCCACCG CTCTACGTAC CCCCTGGCTG CAGCACCGAG 
CTCAACCATC GTGGCTACCA GTTTGTGCAG CGGGTGTTTG AGAAGCATGA TCAGGACCAT 
GATGGTGTCC TCTCACCCAC GGAACTAGAG AGTCTCTTCA GCGTGTTCTC AGTTGCTCCG 
TGGGGCCCTG AACTCTTACA CACAGTCCCC ACTGAGGCTG GCTGTCTGTC CTTGCGTGGA 
TATCTCTGCC AGTGGACCCT GGTGACCTAC TTGGATGTCC AGCACTGCCT TGCCCATCTT 
GGCTACCTGG GTTACCCTAC CCTCTGTGAG CAGGACTCAC AGGCACAGGC TATCACAGTT 
ACCCGCGAGA AGAGACTGGA CCAGGAGAAA GGGCAAACAC AGCGCAGCGT CCTCATGTGT 
AAGGTGTTGG GAGCCCGAGG AGTGGGCAAG TCGGCCTTCT TGCAGGCCTT CCTCGGCCAC 
AGCCTTGGGG AAGCCAGGGA CAGGGACGCC CCTGAGAAAT TGCCCACGCA TACCATCAAT 
ACAGTGCGGG TCAGCGGACA GGAAAAGTAC CTGATTCTGT GTGAAGTGAA TGCTGACAGC 
CTGCTGGACA CCTCTCTAGA CACTACCTGT GATGTCGCCT GCTTAATGTT TGACAGCAGT 
GACCCCGAGA CCTTCGTGCA GTGTGCTACC ATCTACAAGC GTTATTACAT GGATGGGCAG 
ACACCCTGCC TCTTTATCTC CTCCAAAGCT GATCTCCCTG AAGGTGTTGC CCCACCAGGC 
CTATCACCAG CTGAGTTCTG CCGTAGGCAC CGGCTGCCAG CCCCGGCCTC ATTCTCCTGC 
TTGGGACCAG CACAGTCCAG CATAGATGTC TTTACCCAAC TTGCTACTAT GGCCACTTTC 
CCACACCTGG CGCACACAGA GCTGCACCCC ACCCCCTTCT GGCTCCGAGG CGTGCTGGTG 
GCTGTTGGGA CCGCGGTGGC TGCTGTCCTT AGCTTCTCAC TATATAGGGT CCTAGTGAAG 
AGCCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhot2 ( XM_017597069.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452558.1 Rattus norvegicus ras homolog family member T2 (Rhot2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa13076
Clone ID Related Accession (Same CDS sequence)	XM_017597069.1 , NM_181823.1
Accession Version	XM_017597069.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitochondrial Rho GTPase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCGCG ACGTGCGCAT CCTGCTGCTG GGCGAGGCCC AGGTGGGGAA GACTTCTCTG 
ATCCTGTCGC TGGTCGGCGA GGAGTTTCCC GAGGAGGTCC CTGCCCGCGC GGAGGAGATA 
ACCATCCCCG CCGACGTCAC CCCAGAGAAG GTGCCCACCC ATATCGTGGA TTACTCAGAA 
GCGGAGCAGA CAGAGGAGGA ACTTCAGGAG GAGATCCACA AGGCAAACGT GGTGTGTGTG 
GTGTACGATG TGTCTGAGGA AGCCACCATT GAGAAGATCC GAACCAAGTG GATTCCCCTC 
GTGAATGGCA GGACGGCAAC AGGGCCCAGG TTGCCCATCA TCCTGGTAGG CAATAAGTCA 
GACCTGCGGC CAGGGAGTAC CATAGAGGCT GTGTTGCCCA TTATGAGCCA GTTTCCTGAG 
ATAGAGACCT GTGTGGAGTG TTCTGCCAAG CACTTGAGGA ATATCTCAGA GCTGTTCTAC 
TATGCCCAGA AGGCTGTTCT GCATCCCACC GCTCCCCTTT ATGACCCTGA GGCCAAACAG 
TTAAGGCCTG CCTGCGCCCA GGCACTCACA CGCATCTTCA GGCTGTCGGA TCAGGACCTA 
GACCACGCAC TCAGCGACAA GGAGCTCAAT GCCTTCCAGA AGTCCTGCTT TGGCCATCCT 
CTGGCCCCAC AAGCCCTGGA GGATGTAAAG AGGGTAGTGT GCAAGAATGT GGCAGGTGGT 
GTGCAGGATG ACCGACTGAC CCTGGAAGGC TTCCTCTTCC TGAACACACT TTTCATCCAG 
CGTGGCCGGC ATGAGACTAC GTGGACCATC CTACGGCGCT TTGGTTACAG TGACTCGCTG 
GAGCTGACTC CTGACTACCT CTGTCCACCG CTCTACGTAC CCCCTGGCTG CAGCACCGAG 
CTCAACCATC GTGGCTACCA GTTTGTGCAG CGGGTGTTTG AGAAGCATGA TCAGGACCAT 
GATGGTGTCC TCTCACCCAC GGAACTAGAG AGTCTCTTCA GCGTGTTCTC AGTTGCTCCG 
TGGGGCCCTG AACTCTTACA CACAGTCCCC ACTGAGGCTG GCTGTCTGTC CTTGCGTGGA 
TATCTCTGCC AGTGGACCCT GGTGACCTAC TTGGATGTCC AGCACTGCCT TGCCCATCTT 
GGCTACCTGG GTTACCCTAC CCTCTGTGAG CAGGACTCAC AGGCACAGGC TATCACAGTT 
ACCCGCGAGA AGAGACTGGA CCAGGAGAAA GGGCAAACAC AGCGCAGCGT CCTCATGTGT 
AAGGTGTTGG GAGCCCGAGG AGTGGGCAAG TCGGCCTTCT TGCAGGCCTT CCTCGGCCAC 
AGCCTTGGGG AAGCCAGGGA CAGGGACGCC CCTGAGAAAT TGCCCACGCA TACCATCAAT 
ACAGTGCGGG TCAGCGGACA GGAAAAGTAC CTGATTCTGT GTGAAGTGAA TGCTGACAGC 
CTGCTGGACA CCTCTCTAGA CACTACCTGT GATGTCGCCT GCTTAATGTT TGACAGCAGT 
GACCCCGAGA CCTTCGTGCA GTGTGCTACC ATCTACAAGC GTTATTACAT GGATGGGCAG 
ACACCCTGCC TCTTTATCTC CTCCAAAGCT GATCTCCCTG AAGGTGTTGC CCCACCAGGC 
CTATCACCAG CTGAGTTCTG CCGTAGGCAC CGGCTGCCAG CCCCGGCCTC ATTCTCCTGC 
TTGGGACCAG CACAGTCCAG CATAGATGTC TTTACCCAAC TTGCTACTAT GGCCACTTTC 
CCACACCTGG CGCACACAGA GCTGCACCCC ACCCCCTTCT GGCTCCGAGG CGTGCTGGTG 
GCTGTTGGGA CCGCGGTGGC TGCTGTCCTT AGCTTCTCAC TATATAGGGT CCTAGTGAAG 
AGCCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452558.1
CDS	276..2144
Translation	MRRDVRILLLGEAQVGKTSLILSLVGEEFPEEVPARAEEITIPADVTPEKVPTHIVDYSEAEQTEEELQEEIHKANVVCVVYDVSEEATIEKIRTKWIPLVNGRTATGPRLPIILVGNKSDLRPGSTIEAVLPIMSQFPEIETCVECSAKHLRNISELFYYAQKAVLHPTAPLYDPEAKQLRPACAQALTRIFRLSDQDLDHALSDKELNAFQKSCFGHPLAPQALEDVKRVVCKNVAGGVQDDRLTLEGFLFLNTLFIQRGRHETTWTILRRFGYSDSLELTPDYLCPPLYVPPGCSTELNHRGYQFVQRVFEKHDQDHDGVLSPTELESLFSVFSVAPWGPELLHTVPTEAGCLSLRGYLCQWTLVTYLDVQHCLAHLGYLGYPTLCEQDSQAQAITVTREKRLDQEKGQTQRSVLMCKVLGARGVGKSAFLQAFLGHSLGEARDRDAPEKLPTHTINTVRVSGQEKYLILCEVNADSLLDTSLDTTCDVACLMFDSSDPETFVQCATIYKRYYMDGQTPCLFISSKADLPEGVAPPGLSPAEFCRRHRLPAPASFSCLGPAQSSIDVFTQLATMATFPHLAHTELHPTPFWLRGVLVAVGTAVAAVLSFSLYRVLVKSR

Target ORF information:

RefSeq Version	XM_017597069.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ras homolog family member T2 (Rhot2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597069.1

ORF Insert Sequence:

ATGAGGCGCG ACGTGCGCAT CCTGCTGCTG GGCGAGGCCC AGGTGGGGAA GACTTCTCTG 
ATCCTGTCGC TGGTCGGCGA GGAGTTTCCC GAGGAGGTCC CTGCCCGCGC GGAGGAGATA 
ACCATCCCCG CCGACGTCAC CCCAGAGAAG GTGCCCACCC ATATCGTGGA TTACTCAGAA 
GCGGAGCAGA CAGAGGAGGA ACTTCAGGAG GAGATCCACA AGGCAAACGT GGTGTGTGTG 
GTGTACGATG TGTCTGAGGA AGCCACCATT GAGAAGATCC GAACCAAGTG GATTCCCCTC 
GTGAATGGCA GGACGGCAAC AGGGCCCAGG TTGCCCATCA TCCTGGTAGG CAATAAGTCA 
GACCTGCGGC CAGGGAGTAC CATAGAGGCT GTGTTGCCCA TTATGAGCCA GTTTCCTGAG 
ATAGAGACCT GTGTGGAGTG TTCTGCCAAG CACTTGAGGA ATATCTCAGA GCTGTTCTAC 
TATGCCCAGA AGGCTGTTCT GCATCCCACC GCTCCCCTTT ATGACCCTGA GGCCAAACAG 
TTAAGGCCTG CCTGCGCCCA GGCACTCACA CGCATCTTCA GGCTGTCGGA TCAGGACCTA 
GACCACGCAC TCAGCGACAA GGAGCTCAAT GCCTTCCAGA AGTCCTGCTT TGGCCATCCT 
CTGGCCCCAC AAGCCCTGGA GGATGTAAAG AGGGTAGTGT GCAAGAATGT GGCAGGTGGT 
GTGCAGGATG ACCGACTGAC CCTGGAAGGC TTCCTCTTCC TGAACACACT TTTCATCCAG 
CGTGGCCGGC ATGAGACTAC GTGGACCATC CTACGGCGCT TTGGTTACAG TGACTCGCTG 
GAGCTGACTC CTGACTACCT CTGTCCACCG CTCTACGTAC CCCCTGGCTG CAGCACCGAG 
CTCAACCATC GTGGCTACCA GTTTGTGCAG CGGGTGTTTG AGAAGCATGA TCAGGACCAT 
GATGGTGTCC TCTCACCCAC GGAACTAGAG AGTCTCTTCA GCGTGTTCTC AGTTGCTCCG 
TGGGGCCCTG AACTCTTACA CACAGTCCCC ACTGAGGCTG GCTGTCTGTC CTTGCGTGGA 
TATCTCTGCC AGTGGACCCT GGTGACCTAC TTGGATGTCC AGCACTGCCT TGCCCATCTT 
GGCTACCTGG GTTACCCTAC CCTCTGTGAG CAGGACTCAC AGGCACAGGC TATCACAGTT 
ACCCGCGAGA AGAGACTGGA CCAGGAGAAA GGGCAAACAC AGCGCAGCGT CCTCATGTGT 
AAGGTGTTGG GAGCCCGAGG AGTGGGCAAG TCGGCCTTCT TGCAGGCCTT CCTCGGCCAC 
AGCCTTGGGG AAGCCAGGGA CAGGGACGCC CCTGAGAAAT TGCCCACGCA TACCATCAAT 
ACAGTGCGGG TCAGCGGACA GGAAAAGTAC CTGATTCTGT GTGAAGTGAA TGCTGACAGC 
CTGCTGGACA CCTCTCTAGA CACTACCTGT GATGTCGCCT GCTTAATGTT TGACAGCAGT 
GACCCCGAGA CCTTCGTGCA GTGTGCTACC ATCTACAAGC GTTATTACAT GGATGGGCAG 
ACACCCTGCC TCTTTATCTC CTCCAAAGCT GATCTCCCTG AAGGTGTTGC CCCACCAGGC 
CTATCACCAG CTGAGTTCTG CCGTAGGCAC CGGCTGCCAG CCCCGGCCTC ATTCTCCTGC 
TTGGGACCAG CACAGTCCAG CATAGATGTC TTTACCCAAC TTGCTACTAT GGCCACTTTC 
CCACACCTGG CGCACACAGA GCTGCACCCC ACCCCCTTCT GGCTCCGAGG CGTGCTGGTG 
GCTGTTGGGA CCGCGGTGGC TGCTGTCCTT AGCTTCTCAC TATATAGGGT CCTAGTGAAG 
AGCCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of mitochondrial transport in neurons.
Experimental cell research334(1)35-44(2015 May)
Lin MY,Sheng ZH

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

HUMMR, a hypoxia- and HIF-1alpha-inducible protein, alters mitochondrial distribution and transport.
The Journal of cell biology185(6)1065-81(2009 Jun)
Li Y,Lim S,Hoffman D,Aspenstrom P,Federoff HJ,Rempe DA

Bidirectional Ca2+-dependent control of mitochondrial dynamics by the Miro GTPase.
Proceedings of the National Academy of Sciences of the United States of America105(52)20728-33(2008 Dec)
Saotome M,Safiulina D,Szabadkai G,Das S,Fransson A,Aspenstrom P,Rizzuto R,Hajn?czky G

The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in mitochondrial trafficking.
Biochemical and biophysical research communications344(2)500-10(2006 Jun)
Fransson S,Ruusala A,Aspenstr?m P

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Rhot2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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