NAM2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	NAM2
Entrez Gene ID	851098
Full Name	leucine--tRNA ligase NAM2
Synonyms	MSL1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182271.3	NP_013486.3	leucine--tRNA ligase NAM2

Pathways from BioSystems

Homologies

	LARS2	XP_001114552.2
Homo sapiens (human)	LARS2	NP_056155.1
Kluyveromyces lactis	KLLA0E05325g	XP_454185.1
Magnaporthe oryzae (rice blast fungus)	MGG_04042	XP_003719800.1
Arabidopsis thaliana (thale cress)	EMB2369	NP_192344.1
Canis lupus familiaris (dog)	LARS2	XP_005632695.1
Rattus norvegicus (Norway rat)	Lars2	NP_001102257.1
Danio rerio (zebrafish)	lars2	NP_001099171.1
Drosophila melanogaster (fruit fly)	Aats-leu	NP_647932.1
Schizosaccharomyces pombe (fission yeast)	SPAC4G8.09	NP_593069.1
Neurospora crassa	NCU03814	XP_961203.1
Pan troglodytes (chimpanzee)	LARS2	XP_001147749.1
Mus musculus (house mouse)	Lars2	NP_694808.1
Caenorhabditis elegans (roundworm)	lars-2	NP_001021875.1
Saccharomyces cerevisiae (baker's yeast)	NAM2	NP_013486.3
Gallus gallus (chicken)	LARS2	XP_004939517.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002014	XP_321043.4
Eremothecium gossypii	AGOS_AER043C	NP_984903.1
Xenopus tropicalis (tropical clawed frog)	lars2	XP_002938354.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Self-protective responses to norvaline-induced stress in a leucyl-tRNA synthetase editing-deficient yeast strain has been reported.
Title: Self-protective responses to norvaline-induced stress in a leucyl-tRNA synthetase editing-deficient yeast strain.

Data show that the activity of the minimized bI4 intron was enhanced in vitro by the presence of the bI4 maturase or LeuRS.
Title: Leucyl-tRNA synthetase-dependent and -independent activation of a group I intron.

yeast mitochondria have evolved to tolerate lower levels of fidelity in protein synthesis or have developed alternate mechanisms to enhance discrimination of leucine from non-cognate amino acids that can be misactivated by leucyl-tRNA synthetase
Title: A viable amino acid editing activity in the leucyl-tRNA synthetase CP1-splicing domain is not required in the yeast mitochondria.

The following NAM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NAM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00697	NM_001182271.3 Latest version!	Saccharomyces cerevisiae S288C leucine--tRNA ligase NAM2 (NAM2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NAM2 ( NM_001182271.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013486.3 Saccharomyces cerevisiae S288C leucine--tRNA ligase NAM2 (NAM2), partial mRNA.

Price & Availability↑

CloneID	OSi00697
Clone ID Related Accession (Same CDS sequence)	NM_001182271.3
Accession Version	NM_001182271.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	leucine--tRNA ligase NAM2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). On Jul 30, 2012 this sequence version replaced NM_001182271.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCTGTCTC GACCTTCAAG CCGATTCCTA TCCACTAAAA GGGGCCCTGG ACCTGCAGTG 
AAAAAATTAA TTGCAATTGG GGAGAAATGG AAACAGAAGA CAACCCGTGG CTTGCCTAAG 
CAGGATACTC TGAATAGCGG GTCGAAATAT ATTCTGTGCC AGTTTCCATA TCCTTCTGGG 
GCGCTTCACA TAGGACATCT CCGAGTTTAT GTCATTAGCG ACTCTTTGAA TAGATTTTAC 
AAACAAAAAG GGTACAACGT GATACATCCG ATGGGATGGG ATGCTTTTGG GTTACCTGCC 
GAGAACGCTG CTATAGAAAG AAGCATTAAT CCGGCCATAT GGACTAGGGA CAATATTGCA 
AAAATGAAAC AACAAATGCA AAGTATGCTG GCAAATTTTG ATTGGGACAG AGAAATAACT 
ACATGTGATC CCGAATACTA TAAATTCACC CAATGGATTT TCTTAAAACT TTTTGAAAAT 
GGCTTAGCTT ATCGTAAAGA AGCAGAAATT AATTGGGATC CCGTTGATAT GACAGTTTTG 
GCTAATGAAC AAGTGGATGC CCAGGGCCGT TCTTGGAGAT CAGGGGCTAT TGTGGAAAAG 
AAGCAGCTAA AACAGTGGTT TTTGGGAATA ACGAAATTCG CTCCTAAATT GAAAAAGCAC 
TTGAACCAAC TGAAGGACTG GCCTTCCAAC GTGAAGCAAA TGCAAAAAAA TTGGATAGGC 
GAATCTGTGG GTGCAGAATT AGTGTTCAAA GTTGCGGACC CCAAATTTGA AAACTTGATT 
GTCTTTACAA CAAGACCGGA AACTCTTTTT GCTGTACAGT ATGTTGCTCT CGCATTAGAC 
CACCCAATTG TACAAAAATA CTGCGAGGAA ATGCCGGATT TAAAAGAGTT TATACAGAAA 
AGTGATCAAT TACCAAACGA TACGAAGGAA GGATTTCAGT TACCTAACAT AAAGGCCGTA 
AATCCCTTAA CTAAAGAGGA AGTCCCCATA TTTGCAGCTC CATATGTGGT CAGCAGCTAT 
GGTTCAGCAC CTAGTGCAGT AATGGGTTGT CCAGGACACG ATAACCGAGA TTTTGAGTTT 
TGGCAAACAA ATTGTCCTGG TGAACATATC AAGACGTGCA TAGCACCTTT TTTTGACGAT 
GCTTCAAAAG TAACTGAACA GGAAAGACAA AGAATAATTG ATACTGTCCC GTTCACATCT 
ACTGACGGTG TCCTAACCAA AGAATGCGGA GAACACTCAG GAGTTCTCAC TGTAGTGGCA 
AGGAAATCGA TAATGGGGAT GTTGAATAGC GAAGGACTGT CTAAGAGCGT CGTTAGATAC 
AAAATTAGAG ATTGGCTGAT AAGTAGACAA AGATACTGGG GTACTCCAAT ACCCATCATT 
CACTGCGACA ACTGTGGACC TGTCCCCGTT CCAGAAAGTG ATCTACCTGT CAAGCTACCA 
GAACTTGAAG GCTTGGATAC AAAAGGGAAT CCGCTGTCCA CAATCGATGA ATTTGTAAAT 
GTCGCCTGTC CTTCATGCGG GAGCCCTGCG AAAAGAGAAA CTGACACCAT GGATACTTTT 
ATCGATAGTT CTTGGTATTA TTTCAGATTC TTGGATCCCA AAAACACTTC AAAACCATTT 
GATCGTGAAA TTGCAAGTAA AAATATGCCG GTTGATATTT ATATTGGTGG AGTAGAACAT 
GCTATCTTAC ACTTGTTATA CTCAAGGTTT ATTGCTAAAT TTCTCGGATC TATCAATGCA 
TGGAGCGACC CTGCTGGCAT CTTCGAACCA TTCAAAAAAC TGGTGACACA AGGAATGGTT 
CAAGGGAAAA CCTATGTTGA CCCCGATTCT GGTAAATTTT TGAAGCCTGA CGAGTTAACC 
TTTGTAAATG ACTCTCCAGA TGGCAACACA GTGATTATTA AATCAAATGG CAAGGTTCCG 
GTAGTCTCTT ATGAAAAAAT GTCGAAATCT AAATACAATG GTGCAGATCC AAATGAATGT 
ATTTTAAGAC ATGGACCTGA TGCTACGAGA GCACATATCC TCTTCCAAAG TCCAATTGCA 
GATGCCCTGA ATTGGGATGA ATCCAAGATT GTTGGTATAG AACGTTGGTT GCAGAAGGTT 
CTTCATTTGA CCAAAAATAT TCTCAGTCTC GAGAAGGATT TGGCAATAAG TAAAGACTAC 
AAGACCCCGA CCGACTTAAA TGATGCAGAA GTGAAATTTC ACAACGATTT CCAACGCTTC 
TTGAAATCAA TTACGGAATC ATTTGAAGTT AATCTATCGT TAAACACTGT AATATCTGAT 
TATATGAAGC TAACCAATAT TTTAGAAAGT GCATTGAAAA AAGGTGAAGT AAGGAATGAA 
ATGATAGTAC AGAATTTACA AAAGTTGGTA ACCGTTATAT ATCCTGCTGT CCCGTCAATT 
TCAGAAGAAG CTGCAGAGAT GATCAACTCC CAAATGGAAT GGAACCAATA CCGCTGGCCA 
GAAGTTGAGC GCACTACTGA GTCCAAATTC AAAAAGTTTC AAATTGTGGT AAATGGCAGA 
GTCAAATTCA TGTACACGGC TGACAAAAAC TTTTTGAAAT TAGGTAGGGA TGCTGTTATT 
GAAACTTTGA TGAACTTACC GGAAGGGAGA ATGTATTTGA TGAATAAAAA AATCAAAAAA 
TTTGTCATGA AATTCAATGT GATTAGTTTC TTATTCCACA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013486.3
CDS	1..2685
Translation	MLSRPSSRFLSTKRGPGPAVKKLIAIGEKWKQKTTRGLPKQDTLNSGSKYILCQFPYPSGALHIGHLRVYVISDSLNRFYKQKGYNVIHPMGWDAFGLPAENAAIERSINPAIWTRDNIAKMKQQMQSMLANFDWDREITTCDPEYYKFTQWIFLKLFENGLAYRKEAEINWDPVDMTVLANEQVDAQGRSWRSGAIVEKKQLKQWFLGITKFAPKLKKHLNQLKDWPSNVKQMQKNWIGESVGAELVFKVADPKFENLIVFTTRPETLFAVQYVALALDHPIVQKYCEEMPDLKEFIQKSDQLPNDTKEGFQLPNIKAVNPLTKEEVPIFAAPYVVSSYGSAPSAVMGCPGHDNRDFEFWQTNCPGEHIKTCIAPFFDDASKVTEQERQRIIDTVPFTSTDGVLTKECGEHSGVLTVVARKSIMGMLNSEGLSKSVVRYKIRDWLISRQRYWGTPIPIIHCDNCGPVPVPESDLPVKLPELEGLDTKGNPLSTIDEFVNVACPSCGSPAKRETDTMDTFIDSSWYYFRFLDPKNTSKPFDREIASKNMPVDIYIGGVEHAILHLLYSRFIAKFLGSINAWSDPAGIFEPFKKLVTQGMVQGKTYVDPDSGKFLKPDELTFVNDSPDGNTVIIKSNGKVPVVSYEKMSKSKYNGADPNECILRHGPDATRAHILFQSPIADALNWDESKIVGIERWLQKVLHLTKNILSLEKDLAISKDYKTPTDLNDAEVKFHNDFQRFLKSITESFEVNLSLNTVISDYMKLTNILESALKKGEVRNEMIVQNLQKLVTVIYPAVPSISEEAAEMINSQMEWNQYRWPEVERTTESKFKKFQIVVNGRVKFMYTADKNFLKLGRDAVIETLMNLPEGRMYLMNKKIKKFVMKFNVISFLFHK

Target ORF information:

RefSeq Version	NM_001182271.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C leucine--tRNA ligase NAM2 (NAM2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182271.3

ORF Insert Sequence:

ATGCTGTCTC GACCTTCAAG CCGATTCCTA TCCACTAAAA GGGGCCCTGG ACCTGCAGTG 
AAAAAATTAA TTGCAATTGG GGAGAAATGG AAACAGAAGA CAACCCGTGG CTTGCCTAAG 
CAGGATACTC TGAATAGCGG GTCGAAATAT ATTCTGTGCC AGTTTCCATA TCCTTCTGGG 
GCGCTTCACA TAGGACATCT CCGAGTTTAT GTCATTAGCG ACTCTTTGAA TAGATTTTAC 
AAACAAAAAG GGTACAACGT GATACATCCG ATGGGATGGG ATGCTTTTGG GTTACCTGCC 
GAGAACGCTG CTATAGAAAG AAGCATTAAT CCGGCCATAT GGACTAGGGA CAATATTGCA 
AAAATGAAAC AACAAATGCA AAGTATGCTG GCAAATTTTG ATTGGGACAG AGAAATAACT 
ACATGTGATC CCGAATACTA TAAATTCACC CAATGGATTT TCTTAAAACT TTTTGAAAAT 
GGCTTAGCTT ATCGTAAAGA AGCAGAAATT AATTGGGATC CCGTTGATAT GACAGTTTTG 
GCTAATGAAC AAGTGGATGC CCAGGGCCGT TCTTGGAGAT CAGGGGCTAT TGTGGAAAAG 
AAGCAGCTAA AACAGTGGTT TTTGGGAATA ACGAAATTCG CTCCTAAATT GAAAAAGCAC 
TTGAACCAAC TGAAGGACTG GCCTTCCAAC GTGAAGCAAA TGCAAAAAAA TTGGATAGGC 
GAATCTGTGG GTGCAGAATT AGTGTTCAAA GTTGCGGACC CCAAATTTGA AAACTTGATT 
GTCTTTACAA CAAGACCGGA AACTCTTTTT GCTGTACAGT ATGTTGCTCT CGCATTAGAC 
CACCCAATTG TACAAAAATA CTGCGAGGAA ATGCCGGATT TAAAAGAGTT TATACAGAAA 
AGTGATCAAT TACCAAACGA TACGAAGGAA GGATTTCAGT TACCTAACAT AAAGGCCGTA 
AATCCCTTAA CTAAAGAGGA AGTCCCCATA TTTGCAGCTC CATATGTGGT CAGCAGCTAT 
GGTTCAGCAC CTAGTGCAGT AATGGGTTGT CCAGGACACG ATAACCGAGA TTTTGAGTTT 
TGGCAAACAA ATTGTCCTGG TGAACATATC AAGACGTGCA TAGCACCTTT TTTTGACGAT 
GCTTCAAAAG TAACTGAACA GGAAAGACAA AGAATAATTG ATACTGTCCC GTTCACATCT 
ACTGACGGTG TCCTAACCAA AGAATGCGGA GAACACTCAG GAGTTCTCAC TGTAGTGGCA 
AGGAAATCGA TAATGGGGAT GTTGAATAGC GAAGGACTGT CTAAGAGCGT CGTTAGATAC 
AAAATTAGAG ATTGGCTGAT AAGTAGACAA AGATACTGGG GTACTCCAAT ACCCATCATT 
CACTGCGACA ACTGTGGACC TGTCCCCGTT CCAGAAAGTG ATCTACCTGT CAAGCTACCA 
GAACTTGAAG GCTTGGATAC AAAAGGGAAT CCGCTGTCCA CAATCGATGA ATTTGTAAAT 
GTCGCCTGTC CTTCATGCGG GAGCCCTGCG AAAAGAGAAA CTGACACCAT GGATACTTTT 
ATCGATAGTT CTTGGTATTA TTTCAGATTC TTGGATCCCA AAAACACTTC AAAACCATTT 
GATCGTGAAA TTGCAAGTAA AAATATGCCG GTTGATATTT ATATTGGTGG AGTAGAACAT 
GCTATCTTAC ACTTGTTATA CTCAAGGTTT ATTGCTAAAT TTCTCGGATC TATCAATGCA 
TGGAGCGACC CTGCTGGCAT CTTCGAACCA TTCAAAAAAC TGGTGACACA AGGAATGGTT 
CAAGGGAAAA CCTATGTTGA CCCCGATTCT GGTAAATTTT TGAAGCCTGA CGAGTTAACC 
TTTGTAAATG ACTCTCCAGA TGGCAACACA GTGATTATTA AATCAAATGG CAAGGTTCCG 
GTAGTCTCTT ATGAAAAAAT GTCGAAATCT AAATACAATG GTGCAGATCC AAATGAATGT 
ATTTTAAGAC ATGGACCTGA TGCTACGAGA GCACATATCC TCTTCCAAAG TCCAATTGCA 
GATGCCCTGA ATTGGGATGA ATCCAAGATT GTTGGTATAG AACGTTGGTT GCAGAAGGTT 
CTTCATTTGA CCAAAAATAT TCTCAGTCTC GAGAAGGATT TGGCAATAAG TAAAGACTAC 
AAGACCCCGA CCGACTTAAA TGATGCAGAA GTGAAATTTC ACAACGATTT CCAACGCTTC 
TTGAAATCAA TTACGGAATC ATTTGAAGTT AATCTATCGT TAAACACTGT AATATCTGAT 
TATATGAAGC TAACCAATAT TTTAGAAAGT GCATTGAAAA AAGGTGAAGT AAGGAATGAA 
ATGATAGTAC AGAATTTACA AAAGTTGGTA ACCGTTATAT ATCCTGCTGT CCCGTCAATT 
TCAGAAGAAG CTGCAGAGAT GATCAACTCC CAAATGGAAT GGAACCAATA CCGCTGGCCA 
GAAGTTGAGC GCACTACTGA GTCCAAATTC AAAAAGTTTC AAATTGTGGT AAATGGCAGA 
GTCAAATTCA TGTACACGGC TGACAAAAAC TTTTTGAAAT TAGGTAGGGA TGCTGTTATT 
GAAACTTTGA TGAACTTACC GGAAGGGAGA ATGTATTTGA TGAATAAAAA AATCAAAAAA 
TTTGTCATGA AATTCAATGT GATTAGTTTC TTATTCCACA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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NAM2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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