YFL002W-A cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	YFL002W-A
Entrez Gene ID	850548
Full Name	gag-pol fusion protein
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180862.2	NP_116653.1	gag-pol fusion protein

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	YNL054W-B	NP_001268465.1
Saccharomyces cerevisiae (baker's yeast)	YPR137C-A	NP_058192.1
Saccharomyces cerevisiae (baker's yeast)	YDR098C-B	NP_058140.1
Saccharomyces cerevisiae (baker's yeast)	YDR170W-A	NP_010455.1
Saccharomyces cerevisiae (baker's yeast)	YML039W	NP_013672.1
Saccharomyces cerevisiae (baker's yeast)	YHR214C-B	NP_012086.1
Saccharomyces cerevisiae (baker's yeast)	YML045W	NP_013668.1
Saccharomyces cerevisiae (baker's yeast)	YML040W	NP_013673.1
Saccharomyces cerevisiae (baker's yeast)	YMR050C	NP_013765.1
Saccharomyces cerevisiae (baker's yeast)	YPR158W-A	NP_058194.1
Saccharomyces cerevisiae (baker's yeast)	YGR161W-A	NP_058164.1
Saccharomyces cerevisiae (baker's yeast)	YLR410W-A	NP_058175.1
Saccharomyces cerevisiae (baker's yeast)	YER137C-A	NP_058155.1
Saccharomyces cerevisiae (baker's yeast)	YHR214C-C	NP_036195.3
Saccharomyces cerevisiae (baker's yeast)	YPR137C-B	NP_058191.1
Saccharomyces cerevisiae (baker's yeast)	YDR261W-A	NP_058147.3
Saccharomyces cerevisiae (baker's yeast)	YGR038C-B	NP_058160.1
Saccharomyces cerevisiae (baker's yeast)	YBL005W-B	NP_009549.1
Saccharomyces cerevisiae (baker's yeast)	YMR045C	NP_013759.1
Saccharomyces cerevisiae (baker's yeast)	YBR012W-B	NP_009567.1
Saccharomyces cerevisiae (baker's yeast)	YDR210W-B	NP_058142.3
Saccharomyces cerevisiae (baker's yeast)	YDR210C-D	NP_620387.1
Saccharomyces cerevisiae (baker's yeast)	YGR161W-B	NP_058163.1
Saccharomyces cerevisiae (baker's yeast)	YER160C	NP_011087.3
Saccharomyces cerevisiae (baker's yeast)	YGR027W-B	NP_058158.1
Saccharomyces cerevisiae (baker's yeast)	YLR157C-B	NP_058169.1
Saccharomyces cerevisiae (baker's yeast)	YLR256W-A	NP_058173.1
Saccharomyces cerevisiae (baker's yeast)	YBR012W-A	NP_009568.1
Saccharomyces cerevisiae (baker's yeast)	YCL020W	NP_009910.2
Saccharomyces cerevisiae (baker's yeast)	YMR051C	NP_013766.1
Saccharomyces cerevisiae (baker's yeast)	YDR210W-A	NP_058143.1
Saccharomyces cerevisiae (baker's yeast)	YLR227W-A	NP_058172.1
Saccharomyces cerevisiae (baker's yeast)	YGR027W-A	NP_058159.1
Saccharomyces cerevisiae (baker's yeast)	YML045W-A	NP_058176.1
Saccharomyces cerevisiae (baker's yeast)	YPR158C-C	NP_058196.1
Saccharomyces cerevisiae (baker's yeast)	YBL100W-B	NP_009450.1
Saccharomyces cerevisiae (baker's yeast)	YOR343W-B	NP_620389.3
Saccharomyces cerevisiae (baker's yeast)	YDR365W-B	NP_058152.1
Saccharomyces cerevisiae (baker's yeast)	YGR161C-D	NP_058165.3
Saccharomyces cerevisiae (baker's yeast)	YOR192C-B	NP_058185.3
Saccharomyces cerevisiae (baker's yeast)	YLR035C-A	NP_013136.1
Saccharomyces cerevisiae (baker's yeast)	YJL113W	NP_012421.2
Saccharomyces cerevisiae (baker's yeast)	YLR227W-B	NP_058171.1
Saccharomyces cerevisiae (baker's yeast)	YPR158C-D	NP_058195.1
Saccharomyces cerevisiae (baker's yeast)	YPR158W-B	NP_058193.1
Saccharomyces cerevisiae (baker's yeast)	YJR028W	NP_012563.1
Saccharomyces cerevisiae (baker's yeast)	YDR210C-C	NP_620388.3
Saccharomyces cerevisiae (baker's yeast)	YPL257W-A	NP_058190.1
Saccharomyces cerevisiae (baker's yeast)	YDR365W-A	NP_058153.3
Saccharomyces cerevisiae (baker's yeast)	YFL002W-B	NP_058157.1
Saccharomyces cerevisiae (baker's yeast)	YAR009C	NP_009406.1
Saccharomyces cerevisiae (baker's yeast)	YBL100W-A	NP_009451.1
Saccharomyces cerevisiae (baker's yeast)	YPL257W-B	NP_058189.1
Saccharomyces cerevisiae (baker's yeast)	YFL002W-A	NP_116653.1
Saccharomyces cerevisiae (baker's yeast)	YJR029W	NP_012562.1
Saccharomyces cerevisiae (baker's yeast)	YJR027W	NP_012560.1
Saccharomyces cerevisiae (baker's yeast)	YHL009W-B	NP_058133.1
Saccharomyces cerevisiae (baker's yeast)	YNL284C-B	NP_058178.1
Saccharomyces cerevisiae (baker's yeast)	YNL054W-A	NP_058180.1
Saccharomyces cerevisiae (baker's yeast)	YBL005W-A	NP_009550.1
Saccharomyces cerevisiae (baker's yeast)	YNL284C-A	NP_058179.1
Saccharomyces cerevisiae (baker's yeast)	YDR316W-A	NP_058151.1
Saccharomyces cerevisiae (baker's yeast)	YER159C-A	NP_058156.1
Saccharomyces cerevisiae (baker's yeast)	YLR157C-A	NP_058170.1
Saccharomyces cerevisiae (baker's yeast)	YJR026W	NP_012561.3
Saccharomyces cerevisiae (baker's yeast)	YAR010C	NP_009407.1
Saccharomyces cerevisiae (baker's yeast)	YDR034C-D	NP_058138.3
Saccharomyces cerevisiae (baker's yeast)	YLR410W-B	NP_058174.3
Saccharomyces cerevisiae (baker's yeast)	YCL019W	NP_009909.2
Saccharomyces cerevisiae (baker's yeast)	YMR046C	NP_013760.1
Saccharomyces cerevisiae (baker's yeast)	YOR142W-B	NP_058183.1
Saccharomyces cerevisiae (baker's yeast)	YDR316W-B	NP_058150.1
Saccharomyces cerevisiae (baker's yeast)	YDR261W-B	NP_058146.3
Saccharomyces cerevisiae (baker's yeast)	YDR261C-D	NP_058148.1
Saccharomyces cerevisiae (baker's yeast)	YER138C	NP_011064.3
Saccharomyces cerevisiae (baker's yeast)	YOL103W-B	NP_058181.1
Saccharomyces cerevisiae (baker's yeast)	YDR034C-C	NP_058139.1
Saccharomyces cerevisiae (baker's yeast)	YDR098C-A	NP_058141.1
Saccharomyces cerevisiae (baker's yeast)	YOR343W-A	NP_620390.1
Saccharomyces cerevisiae (baker's yeast)	YGR161C-C	NP_058166.1
Saccharomyces cerevisiae (baker's yeast)	YDR261C-C	NP_058149.1
Saccharomyces cerevisiae (baker's yeast)	YOR192C-A	NP_058186.1
Saccharomyces cerevisiae (baker's yeast)	YGR038C-A	NP_058161.1
Saccharomyces cerevisiae (baker's yeast)	YOR142W-A	NP_058184.3
Saccharomyces cerevisiae (baker's yeast)	YOL103W-A	NP_058182.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following YFL002W-A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the YFL002W-A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi178673	NM_001180862.2 Latest version!	Saccharomyces cerevisiae S288C gag-pol fusion protein (YFL002W-A), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

YFL002W-A ( NM_001180862.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116653.1 Saccharomyces cerevisiae S288C gag-pol fusion protein (YFL002W-A), partial mRNA.

Price & Availability↑

CloneID	OSi178673
Clone ID Related Accession (Same CDS sequence)	NM_001180862.2
Accession Version	NM_001180862.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	gag-pol fusion protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). On Feb 18, 2015 this sequence version replaced NM_001180862.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGAATCCC AACAATTACA TCAAAATCCA CATTCTCTAC ATGGTAGCGC CTATGCTTCG 
GTTACTTCTA AGGAAGTCCC ATCAAATCAA GATCCGTTAG CCGTTTCAGC TTCCAATTTA 
CCGGAATTTG ATAGAGATTC CACTAAGGTT AATTCTCAAC AAGAGACAAC ACCTGGGACA 
TCAGCTGTTC CAGAGAACCA TCATCATGTC TCTCCTCAAC CTGCTTCAGT ACCACCTCCA 
CAGAATGGAC AGTACCAACA GCACGGCATG ATGACCCCAA ACAAAGCTAT GGCCTCTAAC 
TGGGCACATT ACCAACAACC GTCTATGATG ACGTGTTCAC ATTATCAAAC GTCACCTGCG 
TATTATCAAC CGGACCCACA CTATCCGTTG CCACAGTATA TCCCACCACT GAGTACTTCC 
TCACCTGATC CAATCGATTC ACAGAATCAA CACTCTGAAG TACCTCAAGC TGAGACAAAG 
GTGAGAAATA ACGTCTTACC ACCACACACT TTAACATCAG AAGAAAACTT TTCTACATGG 
GTTAAATTTT ACATCAGATT TTTGAAGAAC TCTAATCTCG GTGACATCAT TCCAAATGAC 
CAGGGTGAAA TCAAAAGTCA AATGACTTAT GAAGAACATG CGTATATATA CAATACCTTC 
CAAGCATTTG CCCCATTTCA TTTATTGCCA ACATGGGTAA AACAAATTTT AGAAATTAAT 
TATGCTGACA TCCTTACAGT CCTTTGTAAA AGTGTGTCCA AAATGCAAAC TAACAATCAA 
GAATTAAAGG ATTGGATAGC TCTTGCCAAC CTTGAGTACG ACGGAAGTAC ATCTGCTGAT 
ACATTTGAAA TTACAGTCAG TACGATCATT CAGAGGCTAA AAGAAAACAA TATCAATGTT 
AGCGACAGAT TGGCCTGTCA ACTAATACTT AAAGGTCTAT CCGGTGACTT CAAATACCTA 
CGTAATCAAT ATCGTACCAA AACGAACATG AAACTTTCCC AATTATTCGC TGAAATTCAG 
TTAATATATG ACGAAAATAA AATCATGAAT CTAAATAAAC CGTCCCAATA CAAACAACAC 
AGCGAATACA AAAATGTTTC TCGCACATCT CCAAACACGA CTAACACAAA GGTTACAACT 
CGTAATTATC ATAGAACAAA TAGTTCAAAA CCAAGAGCAG CAAAAGCTCA CAATATTGCT 
ACATCTAGTA AATTCTCAAG GGTGAACAAT GATCACATTA ATGAATCAAC CGTTTCATCA 
CAATACTTAA GCGATGACAA CGAACTTAGT CTTGGCCAGC AACAGAAAGA ATCTAAGCCA 
ACACACACAA TAGACTCGAA TGACGAACTA CCTGATCACC TTCTTATTGA TTCAGGAGCT 
TCGCAAACGC TTGTCAGATC AGCCCATTAT TTACACCATG CAACACCCAA TTCTGAAATA 
AACATAGTCG ATGCTCAAAA ACAAGACATT CCTATAAATG CCATTGGTAA TCTTCACTTC 
AACTTTCAGA ACGGCACCAA AACATCAATA AAAGCACTAC ACACACCAAA CATAGCCTAT 
GATCTATTAA GTTTGAGTGA GCTGGCTAAT CAAAATATTA CTGCCTGCTT TACCAGAAAC 
ACTTTAGAAA GATCGGATGG TACAGTACTA GCTCCCATAG TCAAACATGG AGACTTTTAC 
TGGTTATCTA AAAAATACCT AATTCCTTCG CACATTTCAA AGCTAACAAT AAACAACGTC 
AACAAAAGCA AAAGCGTAAA TAAATATCCA TATCCGTTAA TACATCGAAT GCTTGGACAT 
GCTAACTTCC GAAGTATTCA GAAGTCTCTT AAGAAGAATG CAGTTACATA TTTGAAGGAA 
TCGGATATTG AATGGTCTAA CGCTAGCACA TATCAATGTC CTGACTGTCT AATCGGCAAA 
AGCACGAAAC ATAGACATGT CAAAGGATCA CGACTAAAGT ACCAAGAATC ATATGAGCCT 
TTTCAGTACT TGCACACCGA TATATTTGGT CCTGTACATC ACTTACCGAA AAGTGCACCT 
TCTTACTTTA TATCGTTTAC AGATGAGAAA ACCAGATTCC AATGGGTGTA CCCATTACAC 
GACCGTCGTG AAGAATCTAT CCTCAATGTT TTTACATCGA TATTAGCATT TATTAAGAAC 
CAATTCAATG CTCGCGTTCT AGTTATCCAG ATGGATCGTG GCTCCGAGTA CACTAACAAA 
ACTCTTCATA AGTTCTTTAC GAACAGAGGT ATTACTGCAT GCTATACAAC CACGGCAGAT 
TCTAGAGCAC ACGGTGTCGC TGAACGATTA AATCGTACTT TATTAAACGA TTGTCGCACA 
CTGCTTCATT GCAGTGGTCT ACCAAATCAT CTATGGTTCT CAGCAGTCGA ATTTTCTACT 
ATAATCAGAA ATTCATTAGT CTCACCAAAA AACGATAAAT CTGCAAGACA ACATGCAGGT 
TTAGCTGGAC TGGACATTAC TACTATACTA CCTTTCGGTC AACCGGTTAT AGTTAACAAC 
CATAATCCCG ACTCGAAAAT ACATCCTCGT GGCATTCCAG GTTACGCCTT ACATCCGTCA 
CGAAACTCTT ATGGCTATAT TATCTATCTT CCATCATTAA AAAAGACAGT AGATACTACC 
AATTACGTTA TATTACAAGA CAAGCAATCC AAATTGGACC AATTCAATTA TGATACACTC 
ACCTTTGATG ATGATCTCAA TCGTTTAACA GCCCATAACC AATCTTTTAT TGAACAAAAT 
GAAACGGAGC AGTCATATGA TCAAAATACA GAATCTGATC ATGACTATCA ATCGGAGATT 
GAAATAAACT CTGATCCTCT AGTGAACGAC TTCTCGTCCC AATCAATAAA CCCTTTACAA 
TTAGACAAGG AACCAGTCCA AAAAGTACGT GCACCAAAAG AAGTTGATGC CGACATATCT 
GAATACAATA TTCTTCCATC TACTATACGA TCTCGTACAC CCCATATCAT TAATAAAGAG 
AGTACCGAAA TGGGTGGTAC CGTTGAATCA GATACTACTT CACCTAGACA CTCGTCTACC 
TTCACTGCAC GAAACCAAAA CCGACCTGGT AGTACCAATG AGATGATTGA TTTGACCTCA 
CAGGATAGAG TTAATTATGG ACTTGAAAAC ATCAAAACTA CACGTTTGGG TGGTACGGAG 
GAACCATATA TTCAACGAAA TAGTGATACA AATATCAAAT ACAGGACTAC AAATAGTACG 
CCCTCAATAG ATGACCGTTC GTCCAACAGT GAATCCACTA CTCCCATCAT CTCCATAGAA 
ACAAAGGCTG TATGTGATAA TACACCCTCC ATTGATACGG ATCCGCCAGA ATATCGATCT 
TCTGACCATG CGACTCCTAA TATAATGCCT GACAAATCCT CAAAAAATGT TACGGCTGAT 
TCTATTCTTG ACGACCTCCC ACTTCCTGAC TTAACCCATA AATCTCCTAC GGACACTTCT 
GATGTTTCAA AAGATATTCC ACACATACAC TCTCGTCAGA CTAATTCCAG TTTGGGTGGT 
ATGGATGATT CTAATGTTCT GACTACTACC AAAAGTAAGA AAAGATCATT AGAAGATAAT 
GAAACTGAAA TTGAGGTATC CCGAGACACA TGGAATAATA AGAATATGAG AAGTCTGGAA 
CCACCAAGAT CGAAGAAACG CATAAATTTA ATTGCAGCAA TAAAAGGAGT GAAATCGATC 
AAACCAGTTC GAACGACCTT AAGATATGAT GAAGCAATTA CATATAATAA AGACAACAAA 
GAAAAAGACA GATATGTTGA AGCTTATCAT AAAGAAATTA GCCAACTATT GAAAATGAAC 
ACTTGGGATA CAAACAAATA TTATGATAGA AATGACATAG ATCCTAAAAA AGTAATAAAC 
TCAATGTTTA TATTTAACAA GAAACGTGAT GGTACACACA AAGCTAGATT TGTTGCAAGA 
GGCGACATTC AACACCCCGA TACATATGAT TCTGATATGC AATCCAATAC CGTACATCAC 
TATGCACTGA TGACGTCACT GTCAATCGCA TTAGACAACG ACTATTATAT CACACAGCTG 
GACATATCCT CTGCTTACTT ATATGCTGAT ATCAAAGAAG AATTATACAT AAGACCTCCA 
CCACATTTAG GTTTGAATGA TAAATTACTA CGTTTGAGAA AATCACTCTA TGGTTTGAAA 
CAAAGTGGTG CAAACTGGTA TGAAACCATT AAATCATATT TAATAAATTG TTGCGACATG 
CAAGAAGTTC GCGGATGGTC ATGCGTATTT AAGAATAGTC AAGTAACAAT TTGCTTATTC 
GTTGATGATA TGATATTATT CAGCAAAGAC TTAAATGCAA ATAAGAAAAT CATAACAACA 
CTCAAGAAAC AATACGATAC AAAGATAATA AATCTGGGTG AAAGTGATAA CGAAATTCAG 
TACGACATAC TTGGATTAGA GATCAAATAT CAAAGAAGCA AGTACATGAA ATTAGGTATG 
GAAAAATCCT TGACAGAAAA ATTACCCAAA CTAAACGTAC CTTTGAACCC AAAAGGAAAG 
AAACTTAGAG CTCCAGGTCA ACCAGGTCAT TATATAGACC AGGATGAACT AGAAATAGAT 
GAAGATGAAT ACAAAGAGAA AGTACATGAA ATGCAAAAGT TGATTGGTCT AGCTTCATAT 
GTTGGATATA AATTTAGATT TGACTTACTA TACTACATCA ACACACTTGC TCAACATATA 
CTATTCCCCT CTAGGCAAGT TTTAGACATG ACATATGAGT TAATACAATT CATGTGGGAC 
ACTAGAGATA AACAATTAAT ATGGCACAAA AACAAACCTA CCAAGCCAGA TAATAAACTA 
GTCGCAATAA GCGATGCTTC ATATGGTAAC CAACCATATT ACAAGTCACA AATTGGTAAC 
ATTTTCCTAC TCAACGGAAA AGTGATTGGA GGAAAGTCGA CAAAGGCTTC GTTAACATGC 
ACTTCAACTA CAGAAGCAGA AATACACGCA GTCAGTGAAG CTATTCCGCT ATTGAATAAC 
CTCAGTCACC TTGTGCAAGA ACTTAACAAG AAACCAATTA TTAAAGGCTT ACTTACTGAT 
AGTAGATCAA CGATCAGTAT AATTAAGTCT ACAAATGAAG AGAAATTTAG AAACAGATTT 
TTTGGCACAA AGGCAATGAG ACTTAGAGAT GAAGTATCAG GTAATAATTT ATACGTATAC 
TACATCGAGA CCAAGAAGAA CATTGCTGAT GTGATGACAA AACCTCTTCC GATAAAAACA 
TTTAAACTAT TAACTAACAA ATGGATTCAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116653.1
CDS	join(1..1293,1295..5314)
Translation	MESQQLHQNPHSLHGSAYASVTSKEVPSNQDPLAVSASNLPEFDRDSTKVNSQQETTPGTSAVPENHHHVSPQPASVPPPQNGQYQQHGMMTPNKAMASNWAHYQQPSMMTCSHYQTSPAYYQPDPHYPLPQYIPPLSTSSPDPIDSQNQHSEVPQAETKVRNNVLPPHTLTSEENFSTWVKFYIRFLKNSNLGDIIPNDQGEIKSQMTYEEHAYIYNTFQAFAPFHLLPTWVKQILEINYADILTVLCKSVSKMQTNNQELKDWIALANLEYDGSTSADTFEITVSTIIQRLKENNINVSDRLACQLILKGLSGDFKYLRNQYRTKTNMKLSQLFAEIQLIYDENKIMNLNKPSQYKQHSEYKNVSRTSPNTTNTKVTTRNYHRTNSSKPRAAKAHNIATSSKFSRVNNDHINESTVSSQYLSDDNELSLGQQQKESKPTHTIDSNDELPDHLLIDSGASQTLVRSAHYLHHATPNSEINIVDAQKQDIPINAIGNLHFNFQNGTKTSIKALHTPNIAYDLLSLSELANQNITACFTRNTLERSDGTVLAPIVKHGDFYWLSKKYLIPSHISKLTINNVNKSKSVNKYPYPLIHRMLGHANFRSIQKSLKKNAVTYLKESDIEWSNASTYQCPDCLIGKSTKHRHVKGSRLKYQESYEPFQYLHTDIFGPVHHLPKSAPSYFISFTDEKTRFQWVYPLHDRREESILNVFTSILAFIKNQFNARVLVIQMDRGSEYTNKTLHKFFTNRGITACYTTTADSRAHGVAERLNRTLLNDCRTLLHCSGLPNHLWFSAVEFSTIIRNSLVSPKNDKSARQHAGLAGLDITTILPFGQPVIVNNHNPDSKIHPRGIPGYALHPSRNSYGYIIYLPSLKKTVDTTNYVILQDKQSKLDQFNYDTLTFDDDLNRLTAHNQSFIEQNETEQSYDQNTESDHDYQSEIEINSDPLVNDFSSQSINPLQLDKEPVQKVRAPKEVDADISEYNILPSTIRSRTPHIINKESTEMGGTVESDTTSPRHSSTFTARNQNRPGSTNEMIDLTSQDRVNYGLENIKTTRLGGTEEPYIQRNSDTNIKYRTTNSTPSIDDRSSNSESTTPIISIETKAVCDNTPSIDTDPPEYRSSDHATPNIMPDKSSKNVTADSILDDLPLPDLTHKSPTDTSDVSKDIPHIHSRQTNSSLGGMDDSNVLTTTKSKKRSLEDNETEIEVSRDTWNNKNMRSLEPPRSKKRINLIAAIKGVKSIKPVRTTLRYDEAITYNKDNKEKDRYVEAYHKEISQLLKMNTWDTNKYYDRNDIDPKKVINSMFIFNKKRDGTHKARFVARGDIQHPDTYDSDMQSNTVHHYALMTSLSIALDNDYYITQLDISSAYLYADIKEELYIRPPPHLGLNDKLLRLRKSLYGLKQSGANWYETIKSYLINCCDMQEVRGWSCVFKNSQVTICLFVDDMILFSKDLNANKKIITTLKKQYDTKIINLGESDNEIQYDILGLEIKYQRSKYMKLGMEKSLTEKLPKLNVPLNPKGKKLRAPGQPGHYIDQDELEIDEDEYKEKVHEMQKLIGLASYVGYKFRFDLLYYINTLAQHILFPSRQVLDMTYELIQFMWDTRDKQLIWHKNKPTKPDNKLVAISDASYGNQPYYKSQIGNIFLLNGKVIGGKSTKASLTCTSTTEAEIHAVSEAIPLLNNLSHLVQELNKKPIIKGLLTDSRSTISIIKSTNEEKFRNRFFGTKAMRLRDEVSGNNLYVYYIETKKNIADVMTKPLPIKTFKLLTNKWIH

Target ORF information:

RefSeq Version	NM_001180862.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C gag-pol fusion protein (YFL002W-A), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180862.2

ORF Insert Sequence:

ATGGAATCCC AACAATTACA TCAAAATCCA CATTCTCTAC ATGGTAGCGC CTATGCTTCG 
GTTACTTCTA AGGAAGTCCC ATCAAATCAA GATCCGTTAG CCGTTTCAGC TTCCAATTTA 
CCGGAATTTG ATAGAGATTC CACTAAGGTT AATTCTCAAC AAGAGACAAC ACCTGGGACA 
TCAGCTGTTC CAGAGAACCA TCATCATGTC TCTCCTCAAC CTGCTTCAGT ACCACCTCCA 
CAGAATGGAC AGTACCAACA GCACGGCATG ATGACCCCAA ACAAAGCTAT GGCCTCTAAC 
TGGGCACATT ACCAACAACC GTCTATGATG ACGTGTTCAC ATTATCAAAC GTCACCTGCG 
TATTATCAAC CGGACCCACA CTATCCGTTG CCACAGTATA TCCCACCACT GAGTACTTCC 
TCACCTGATC CAATCGATTC ACAGAATCAA CACTCTGAAG TACCTCAAGC TGAGACAAAG 
GTGAGAAATA ACGTCTTACC ACCACACACT TTAACATCAG AAGAAAACTT TTCTACATGG 
GTTAAATTTT ACATCAGATT TTTGAAGAAC TCTAATCTCG GTGACATCAT TCCAAATGAC 
CAGGGTGAAA TCAAAAGTCA AATGACTTAT GAAGAACATG CGTATATATA CAATACCTTC 
CAAGCATTTG CCCCATTTCA TTTATTGCCA ACATGGGTAA AACAAATTTT AGAAATTAAT 
TATGCTGACA TCCTTACAGT CCTTTGTAAA AGTGTGTCCA AAATGCAAAC TAACAATCAA 
GAATTAAAGG ATTGGATAGC TCTTGCCAAC CTTGAGTACG ACGGAAGTAC ATCTGCTGAT 
ACATTTGAAA TTACAGTCAG TACGATCATT CAGAGGCTAA AAGAAAACAA TATCAATGTT 
AGCGACAGAT TGGCCTGTCA ACTAATACTT AAAGGTCTAT CCGGTGACTT CAAATACCTA 
CGTAATCAAT ATCGTACCAA AACGAACATG AAACTTTCCC AATTATTCGC TGAAATTCAG 
TTAATATATG ACGAAAATAA AATCATGAAT CTAAATAAAC CGTCCCAATA CAAACAACAC 
AGCGAATACA AAAATGTTTC TCGCACATCT CCAAACACGA CTAACACAAA GGTTACAACT 
CGTAATTATC ATAGAACAAA TAGTTCAAAA CCAAGAGCAG CAAAAGCTCA CAATATTGCT 
ACATCTAGTA AATTCTCAAG GGTGAACAAT GATCACATTA ATGAATCAAC CGTTTCATCA 
CAATACTTAA GCGATGACAA CGAACTTAGT CTTGGCCAGC AACAGAAAGA ATCTAAGCCA 
ACACACACAA TAGACTCGAA TGACGAACTA CCTGATCACC TTCTTATTGA TTCAGGAGCT 
TCGCAAACGC TTGTCAGATC AGCCCATTAT TTACACCATG CAACACCCAA TTCTGAAATA 
AACATAGTCG ATGCTCAAAA ACAAGACATT CCTATAAATG CCATTGGTAA TCTTCACTTC 
AACTTTCAGA ACGGCACCAA AACATCAATA AAAGCACTAC ACACACCAAA CATAGCCTAT 
GATCTATTAA GTTTGAGTGA GCTGGCTAAT CAAAATATTA CTGCCTGCTT TACCAGAAAC 
ACTTTAGAAA GATCGGATGG TACAGTACTA GCTCCCATAG TCAAACATGG AGACTTTTAC 
TGGTTATCTA AAAAATACCT AATTCCTTCG CACATTTCAA AGCTAACAAT AAACAACGTC 
AACAAAAGCA AAAGCGTAAA TAAATATCCA TATCCGTTAA TACATCGAAT GCTTGGACAT 
GCTAACTTCC GAAGTATTCA GAAGTCTCTT AAGAAGAATG CAGTTACATA TTTGAAGGAA 
TCGGATATTG AATGGTCTAA CGCTAGCACA TATCAATGTC CTGACTGTCT AATCGGCAAA 
AGCACGAAAC ATAGACATGT CAAAGGATCA CGACTAAAGT ACCAAGAATC ATATGAGCCT 
TTTCAGTACT TGCACACCGA TATATTTGGT CCTGTACATC ACTTACCGAA AAGTGCACCT 
TCTTACTTTA TATCGTTTAC AGATGAGAAA ACCAGATTCC AATGGGTGTA CCCATTACAC 
GACCGTCGTG AAGAATCTAT CCTCAATGTT TTTACATCGA TATTAGCATT TATTAAGAAC 
CAATTCAATG CTCGCGTTCT AGTTATCCAG ATGGATCGTG GCTCCGAGTA CACTAACAAA 
ACTCTTCATA AGTTCTTTAC GAACAGAGGT ATTACTGCAT GCTATACAAC CACGGCAGAT 
TCTAGAGCAC ACGGTGTCGC TGAACGATTA AATCGTACTT TATTAAACGA TTGTCGCACA 
CTGCTTCATT GCAGTGGTCT ACCAAATCAT CTATGGTTCT CAGCAGTCGA ATTTTCTACT 
ATAATCAGAA ATTCATTAGT CTCACCAAAA AACGATAAAT CTGCAAGACA ACATGCAGGT 
TTAGCTGGAC TGGACATTAC TACTATACTA CCTTTCGGTC AACCGGTTAT AGTTAACAAC 
CATAATCCCG ACTCGAAAAT ACATCCTCGT GGCATTCCAG GTTACGCCTT ACATCCGTCA 
CGAAACTCTT ATGGCTATAT TATCTATCTT CCATCATTAA AAAAGACAGT AGATACTACC 
AATTACGTTA TATTACAAGA CAAGCAATCC AAATTGGACC AATTCAATTA TGATACACTC 
ACCTTTGATG ATGATCTCAA TCGTTTAACA GCCCATAACC AATCTTTTAT TGAACAAAAT 
GAAACGGAGC AGTCATATGA TCAAAATACA GAATCTGATC ATGACTATCA ATCGGAGATT 
GAAATAAACT CTGATCCTCT AGTGAACGAC TTCTCGTCCC AATCAATAAA CCCTTTACAA 
TTAGACAAGG AACCAGTCCA AAAAGTACGT GCACCAAAAG AAGTTGATGC CGACATATCT 
GAATACAATA TTCTTCCATC TACTATACGA TCTCGTACAC CCCATATCAT TAATAAAGAG 
AGTACCGAAA TGGGTGGTAC CGTTGAATCA GATACTACTT CACCTAGACA CTCGTCTACC 
TTCACTGCAC GAAACCAAAA CCGACCTGGT AGTACCAATG AGATGATTGA TTTGACCTCA 
CAGGATAGAG TTAATTATGG ACTTGAAAAC ATCAAAACTA CACGTTTGGG TGGTACGGAG 
GAACCATATA TTCAACGAAA TAGTGATACA AATATCAAAT ACAGGACTAC AAATAGTACG 
CCCTCAATAG ATGACCGTTC GTCCAACAGT GAATCCACTA CTCCCATCAT CTCCATAGAA 
ACAAAGGCTG TATGTGATAA TACACCCTCC ATTGATACGG ATCCGCCAGA ATATCGATCT 
TCTGACCATG CGACTCCTAA TATAATGCCT GACAAATCCT CAAAAAATGT TACGGCTGAT 
TCTATTCTTG ACGACCTCCC ACTTCCTGAC TTAACCCATA AATCTCCTAC GGACACTTCT 
GATGTTTCAA AAGATATTCC ACACATACAC TCTCGTCAGA CTAATTCCAG TTTGGGTGGT 
ATGGATGATT CTAATGTTCT GACTACTACC AAAAGTAAGA AAAGATCATT AGAAGATAAT 
GAAACTGAAA TTGAGGTATC CCGAGACACA TGGAATAATA AGAATATGAG AAGTCTGGAA 
CCACCAAGAT CGAAGAAACG CATAAATTTA ATTGCAGCAA TAAAAGGAGT GAAATCGATC 
AAACCAGTTC GAACGACCTT AAGATATGAT GAAGCAATTA CATATAATAA AGACAACAAA 
GAAAAAGACA GATATGTTGA AGCTTATCAT AAAGAAATTA GCCAACTATT GAAAATGAAC 
ACTTGGGATA CAAACAAATA TTATGATAGA AATGACATAG ATCCTAAAAA AGTAATAAAC 
TCAATGTTTA TATTTAACAA GAAACGTGAT GGTACACACA AAGCTAGATT TGTTGCAAGA 
GGCGACATTC AACACCCCGA TACATATGAT TCTGATATGC AATCCAATAC CGTACATCAC 
TATGCACTGA TGACGTCACT GTCAATCGCA TTAGACAACG ACTATTATAT CACACAGCTG 
GACATATCCT CTGCTTACTT ATATGCTGAT ATCAAAGAAG AATTATACAT AAGACCTCCA 
CCACATTTAG GTTTGAATGA TAAATTACTA CGTTTGAGAA AATCACTCTA TGGTTTGAAA 
CAAAGTGGTG CAAACTGGTA TGAAACCATT AAATCATATT TAATAAATTG TTGCGACATG 
CAAGAAGTTC GCGGATGGTC ATGCGTATTT AAGAATAGTC AAGTAACAAT TTGCTTATTC 
GTTGATGATA TGATATTATT CAGCAAAGAC TTAAATGCAA ATAAGAAAAT CATAACAACA 
CTCAAGAAAC AATACGATAC AAAGATAATA AATCTGGGTG AAAGTGATAA CGAAATTCAG 
TACGACATAC TTGGATTAGA GATCAAATAT CAAAGAAGCA AGTACATGAA ATTAGGTATG 
GAAAAATCCT TGACAGAAAA ATTACCCAAA CTAAACGTAC CTTTGAACCC AAAAGGAAAG 
AAACTTAGAG CTCCAGGTCA ACCAGGTCAT TATATAGACC AGGATGAACT AGAAATAGAT 
GAAGATGAAT ACAAAGAGAA AGTACATGAA ATGCAAAAGT TGATTGGTCT AGCTTCATAT 
GTTGGATATA AATTTAGATT TGACTTACTA TACTACATCA ACACACTTGC TCAACATATA 
CTATTCCCCT CTAGGCAAGT TTTAGACATG ACATATGAGT TAATACAATT CATGTGGGAC 
ACTAGAGATA AACAATTAAT ATGGCACAAA AACAAACCTA CCAAGCCAGA TAATAAACTA 
GTCGCAATAA GCGATGCTTC ATATGGTAAC CAACCATATT ACAAGTCACA AATTGGTAAC 
ATTTTCCTAC TCAACGGAAA AGTGATTGGA GGAAAGTCGA CAAAGGCTTC GTTAACATGC 
ACTTCAACTA CAGAAGCAGA AATACACGCA GTCAGTGAAG CTATTCCGCT ATTGAATAAC 
CTCAGTCACC TTGTGCAAGA ACTTAACAAG AAACCAATTA TTAAAGGCTT ACTTACTGAT 
AGTAGATCAA CGATCAGTAT AATTAAGTCT ACAAATGAAG AGAAATTTAG AAACAGATTT 
TTTGGCACAA AGGCAATGAG ACTTAGAGAT GAAGTATCAG GTAATAATTT ATACGTATAC 
TACATCGAGA CCAAGAAGAA CATTGCTGAT GTGATGACAA AACCTCTTCC GATAAAAACA 
TTTAAACTAT TAACTAACAA ATGGATTCAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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YFL002W-A cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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