YHR214C-B cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	YHR214C-B
Entrez Gene ID	856623
Full Name	gag-pol fusion protein
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181431.2	NP_012086.1	gag-pol fusion protein

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	YNL054W-B	NP_001268465.1
Saccharomyces cerevisiae (baker's yeast)	YPR137C-A	NP_058192.1
Saccharomyces cerevisiae (baker's yeast)	YDR098C-B	NP_058140.1
Saccharomyces cerevisiae (baker's yeast)	YDR170W-A	NP_010455.1
Saccharomyces cerevisiae (baker's yeast)	YML039W	NP_013672.1
Saccharomyces cerevisiae (baker's yeast)	YHR214C-B	NP_012086.1
Saccharomyces cerevisiae (baker's yeast)	YML045W	NP_013668.1
Saccharomyces cerevisiae (baker's yeast)	YML040W	NP_013673.1
Saccharomyces cerevisiae (baker's yeast)	YMR050C	NP_013765.1
Saccharomyces cerevisiae (baker's yeast)	YPR158W-A	NP_058194.1
Saccharomyces cerevisiae (baker's yeast)	YGR161W-A	NP_058164.1
Saccharomyces cerevisiae (baker's yeast)	YLR410W-A	NP_058175.1
Saccharomyces cerevisiae (baker's yeast)	YER137C-A	NP_058155.1
Saccharomyces cerevisiae (baker's yeast)	YHR214C-C	NP_036195.3
Saccharomyces cerevisiae (baker's yeast)	YPR137C-B	NP_058191.1
Saccharomyces cerevisiae (baker's yeast)	YDR261W-A	NP_058147.3
Saccharomyces cerevisiae (baker's yeast)	YGR038C-B	NP_058160.1
Saccharomyces cerevisiae (baker's yeast)	YBL005W-B	NP_009549.1
Saccharomyces cerevisiae (baker's yeast)	YMR045C	NP_013759.1
Saccharomyces cerevisiae (baker's yeast)	YBR012W-B	NP_009567.1
Saccharomyces cerevisiae (baker's yeast)	YDR210W-B	NP_058142.3
Saccharomyces cerevisiae (baker's yeast)	YDR210C-D	NP_620387.1
Saccharomyces cerevisiae (baker's yeast)	YGR161W-B	NP_058163.1
Saccharomyces cerevisiae (baker's yeast)	YER160C	NP_011087.3
Saccharomyces cerevisiae (baker's yeast)	YGR027W-B	NP_058158.1
Saccharomyces cerevisiae (baker's yeast)	YLR157C-B	NP_058169.1
Saccharomyces cerevisiae (baker's yeast)	YLR256W-A	NP_058173.1
Saccharomyces cerevisiae (baker's yeast)	YBR012W-A	NP_009568.1
Saccharomyces cerevisiae (baker's yeast)	YCL020W	NP_009910.2
Saccharomyces cerevisiae (baker's yeast)	YMR051C	NP_013766.1
Saccharomyces cerevisiae (baker's yeast)	YDR210W-A	NP_058143.1
Saccharomyces cerevisiae (baker's yeast)	YLR227W-A	NP_058172.1
Saccharomyces cerevisiae (baker's yeast)	YGR027W-A	NP_058159.1
Saccharomyces cerevisiae (baker's yeast)	YML045W-A	NP_058176.1
Saccharomyces cerevisiae (baker's yeast)	YPR158C-C	NP_058196.1
Saccharomyces cerevisiae (baker's yeast)	YBL100W-B	NP_009450.1
Saccharomyces cerevisiae (baker's yeast)	YOR343W-B	NP_620389.3
Saccharomyces cerevisiae (baker's yeast)	YDR365W-B	NP_058152.1
Saccharomyces cerevisiae (baker's yeast)	YGR161C-D	NP_058165.3
Saccharomyces cerevisiae (baker's yeast)	YOR192C-B	NP_058185.3
Saccharomyces cerevisiae (baker's yeast)	YLR035C-A	NP_013136.1
Saccharomyces cerevisiae (baker's yeast)	YJL113W	NP_012421.2
Saccharomyces cerevisiae (baker's yeast)	YLR227W-B	NP_058171.1
Saccharomyces cerevisiae (baker's yeast)	YPR158C-D	NP_058195.1
Saccharomyces cerevisiae (baker's yeast)	YPR158W-B	NP_058193.1
Saccharomyces cerevisiae (baker's yeast)	YJR028W	NP_012563.1
Saccharomyces cerevisiae (baker's yeast)	YDR210C-C	NP_620388.3
Saccharomyces cerevisiae (baker's yeast)	YPL257W-A	NP_058190.1
Saccharomyces cerevisiae (baker's yeast)	YDR365W-A	NP_058153.3
Saccharomyces cerevisiae (baker's yeast)	YFL002W-B	NP_058157.1
Saccharomyces cerevisiae (baker's yeast)	YAR009C	NP_009406.1
Saccharomyces cerevisiae (baker's yeast)	YBL100W-A	NP_009451.1
Saccharomyces cerevisiae (baker's yeast)	YPL257W-B	NP_058189.1
Saccharomyces cerevisiae (baker's yeast)	YFL002W-A	NP_116653.1
Saccharomyces cerevisiae (baker's yeast)	YJR029W	NP_012562.1
Saccharomyces cerevisiae (baker's yeast)	YJR027W	NP_012560.1
Saccharomyces cerevisiae (baker's yeast)	YHL009W-B	NP_058133.1
Saccharomyces cerevisiae (baker's yeast)	YNL284C-B	NP_058178.1
Saccharomyces cerevisiae (baker's yeast)	YNL054W-A	NP_058180.1
Saccharomyces cerevisiae (baker's yeast)	YBL005W-A	NP_009550.1
Saccharomyces cerevisiae (baker's yeast)	YNL284C-A	NP_058179.1
Saccharomyces cerevisiae (baker's yeast)	YDR316W-A	NP_058151.1
Saccharomyces cerevisiae (baker's yeast)	YER159C-A	NP_058156.1
Saccharomyces cerevisiae (baker's yeast)	YLR157C-A	NP_058170.1
Saccharomyces cerevisiae (baker's yeast)	YJR026W	NP_012561.3
Saccharomyces cerevisiae (baker's yeast)	YAR010C	NP_009407.1
Saccharomyces cerevisiae (baker's yeast)	YDR034C-D	NP_058138.3
Saccharomyces cerevisiae (baker's yeast)	YLR410W-B	NP_058174.3
Saccharomyces cerevisiae (baker's yeast)	YCL019W	NP_009909.2
Saccharomyces cerevisiae (baker's yeast)	YMR046C	NP_013760.1
Saccharomyces cerevisiae (baker's yeast)	YOR142W-B	NP_058183.1
Saccharomyces cerevisiae (baker's yeast)	YDR316W-B	NP_058150.1
Saccharomyces cerevisiae (baker's yeast)	YDR261W-B	NP_058146.3
Saccharomyces cerevisiae (baker's yeast)	YDR261C-D	NP_058148.1
Saccharomyces cerevisiae (baker's yeast)	YER138C	NP_011064.3
Saccharomyces cerevisiae (baker's yeast)	YOL103W-B	NP_058181.1
Saccharomyces cerevisiae (baker's yeast)	YDR034C-C	NP_058139.1
Saccharomyces cerevisiae (baker's yeast)	YDR098C-A	NP_058141.1
Saccharomyces cerevisiae (baker's yeast)	YOR343W-A	NP_620390.1
Saccharomyces cerevisiae (baker's yeast)	YGR161C-C	NP_058166.1
Saccharomyces cerevisiae (baker's yeast)	YDR261C-C	NP_058149.1
Saccharomyces cerevisiae (baker's yeast)	YOR192C-A	NP_058186.1
Saccharomyces cerevisiae (baker's yeast)	YGR038C-A	NP_058161.1
Saccharomyces cerevisiae (baker's yeast)	YOR142W-A	NP_058184.3
Saccharomyces cerevisiae (baker's yeast)	YOL103W-A	NP_058182.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following YHR214C-B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the YHR214C-B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi178691	NM_001181431.2 Latest version!	Saccharomyces cerevisiae S288C gag-pol fusion protein (YHR214C-B), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

YHR214C-B ( NM_001181431.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012086.1 Saccharomyces cerevisiae S288C gag-pol fusion protein (YHR214C-B), partial mRNA.

Price & Availability↑

CloneID	OSi178691
Clone ID Related Accession (Same CDS sequence)	NM_001181431.2
Accession Version	NM_001181431.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	gag-pol fusion protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). On Feb 18, 2015 this sequence version replaced NM_001181431.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGAATCCC AACAATTATC TAATTACCCA CATATATCTC ATGGTAGCGC CTGTGCTTCG 
GTTACTTCTA AGGAAGTCCA CACAAATCAA GATCCGTTAG ACGTTTCAGC TTCCAAAATT 
CAAGAATATG ATAAGGCTTC CACTAAGGCT AACTCTCAAC AGACAACAAC ACCTGCTTCA 
TCAGCTGTTC CAGAGAACCT CCATCATGCC TCTCCTCAAC CTGCTTCAGT ACCACCTCCA 
CAGAATGGGC CGTACCCACA GCAGTGCATG ATGACCCAAA ACCAAGCCAA TCCATCTGGT 
TGGTCATTTT ACGGACACCC ATCTATGATT CCGTATACAC CTTATCAAAT GTCGCCTATG 
TACTTTCCAC CTGGGCCACA ATCACAGTTT CCGCAGTATC CATCATCAGT TGGAACGCCT 
CTGAGCACTC CATCACCTGA GTCAGGTAAT ACATTTACTG ATTCATCCTC AGCGGACTCT 
GATATGACAT CCACTAAAAA ATATGTCAGA CCACCACCAA TGTTAACCTC ACCTAATGAC 
TTTCCAAATT GGGTTAAAAC ATACATCAAA TTTTTACAAA ACTCGAATCT CGGTGGTATT 
ATTCCGACAG TAAACGGAAA ACCCGTACCA CCAATGTTAA CCTCACCTAA TGACTTTCCA 
AATTGGGTTA AAACATACAT CAAATTTTTA CAAAACTCGA ATCTCGGTGG TATTATTCCG 
ACAGTAAACG GAAAACCCGT ACGTCAGATC ACTGATGATG AACTCACCTT CTTGTATAAC 
ACTTTTCAAA TATTTGCTCC CTCTCAATTC CTACCTACCT GGGTCAAAGA CATCCTATCC 
GTTGATTATA CGGATATCAT GAAAATTCTT TCCAAAAGTA TTGAAAAAAT GCAATCTGAT 
ACCCAAGAGG CAAACGACAT TGTGACCCTG GCAAATTTGC AATATAATGG CAGTACACCT 
GCAGATGCAT TTGAAACAAA AGTCACAAAC ATTATCGACA GACTGAACAA TAATGGCATT 
CATATCAATA ACAAGGTCGC ATGCCAATTA ATTATGAGAG GTCTATCTGG CGAATATAAA 
TTTTTACGCT ACACACGTCA TCGACATCTA AATATGACAG TCGCTGAACT GTTCTTAGAT 
ATCCATGCTA TTTATGAAGA ACAACAGGGA TCGAGAAACA GCAAACCTAA TTACAGGAGA 
AATCCGAGTG ATGAGAAGAA TGATTCTCGC AGCTATACGA ATACAACCAA ACCCAAAGTT 
ATAGCTCGGA ATCCTCAAAA AACAAATAAT TCGAAATCGA AAACAGCCAG GGCTCACAAT 
GTATCCACAT CTAATAACTC TCCCAGCACG GACAACGATT CCATCAGTAA ATCAACTACT 
GAACCGATTC AATTGAACAA TAAGCACGAC CTTCATTTAG GCCAGAAACT TACTGAATCT 
ACAGTAAATC ATACTAATCA TTCTGATGAT GAACTCCCTG GACACCTCCT TCTCGATTCA 
GGAGCATCAC GAACCCTTAT AAGATCTGCT CATCACATAC ACTCAGCATC ATCTAATCCT 
GACATAAACG TAGTTGATGC TCAAAAAAGA AATATACCAA TTAACGCTAT TGGTGACCTA 
CAATTTCACT TCCAGGACAA CACCAAAACA TCAATAAAGG TATTGCACAC TCCTAACATA 
GCCTATGACT TACTCAGTTT GAATGAATTG GCTGCAGTAG ATATCACAGC ATGCTTTACC 
AAAAACGTCT TAGAACGGTC TGACGGCACT GTACTTGCAC CTATCGTAAA ATATGGAGAC 
TTTTACTGGG TATCTAAAAA GTACTTGCTT CCATCAAATA TCTCCGTACC CACCATCAAT 
AATGTCCATA CAAGTGAAAG TACACGCAAA TATCCTTATC CTTTCATTCA TCGAATGCTT 
GCGCATGCCA ATGCACAGAC AATTCGATAC TCACTTAAAA ATAACACCAT CACGTATTTT 
AACGAATCAG ATGTCGACTG GTCTAGTGCT ATTGACTATC AATGTCCTGA TTGTTTAATC 
GGCAAAAGCA CCAAACACAG ACATATCAAA GGTTCACGAC TAAAATACCA AAATTCATAC 
GAACCCTTTC AATACCTACA TACTGACATA TTTGGTCCAG TTCACAACCT ACCAAAAAGT 
GCACCATCCT ATTTCATCTC ATTTACTGAT GAGACAACAA AATTCCGTTG GGTTTATCCA 
TTACACGACC GTCGCGAGGA CTCTATCCTC GATGTTTTTA CTACGATACT AGCTTTTATT 
AAAAACCAGT TTCAGGCCAG TGTCTTGGTT ATACAAATGG ACCGTGGTTC TGAGTATACT 
AACAGAACTC TCCATAAATT CCTTGAAAAA AATGGTATAA CTCCATGCTA TACAACCACA 
GCGGATTCCC GAGCACATGG AGTCGCTGAA CGGCTCAACC GTACCTTATT AGATGACTGC 
CGTACTCAAC TGCAATGTAG TGGTTTACCG AACCATTTAT GGTTCTCTGC AATCGAATTT 
TCTACTATTG TGAGAAATTC ACTAGCTTCA CCTAAAAGCA AAAAATCTGC AAGACAACAT 
GCTGGCTTGG CAGGACTTGA TATCAGTACT TTGTTACCTT TCGGTCAACC TGTTATCGTC 
AATGATCACA ACCCTAACTC CAAAATACAT CCTCGTGGCA TCCCAGGCTA CGCTCTACAT 
CCGTCTCGAA ACTCTTATGG ATATATCATC TATCTTCCGT CCTTAAAGAA GACAGTAGAT 
ACAACTAACT ATGTTATTCT TCAGGGCAAG GAATCCAGAT TAGATCAATT CAATTACGAC 
GCACTCACTT TCGATGAAGA CTTAAACCGT TTAACTGCTT CATATCATTC GTTCATTGCG 
TCAAATGAGA TCCAACAATC CAATGATCTT AACATAGAAT CTGACCATGA CTTCCAATCT 
GACATTGAAC TACATCCTGA GCAACTGAGA AATGTCCTTT CAAAAGCTGT GAGTCCAACC 
GATTCCACAC CTCCGTCAAC TCATACTGAA GATTCGAAAC GTGTTTCCAA AACCAATATT 
CGCGCACCCA GAGAAGTTGA CCCCAACATA TCTGAATCTA ATATTCTTCC ATCTAAGAAG 
AGATCTAGCA CCCCCCAAAT TTCCGATATC GAGAGTACCG GTTCGGGTGG TATGCATAGA 
TTAGATGTTC CTTTACTTGC TCCCATGTCC CAATCTAACA CACATGAGTC GTCGCACGCC 
AGTAAATCTA AAGATTTCAG ACACTCAGAC TCGTACAGTG ACAATGAGAC TAATCATACA 
AACGTACCAA TATCCAGTAC GGGTGGTACC AATAATAAAA CTGTTCCGCA GACAAGCGAA 
CAGGAGACTG AGAAAAGGAT CATACACCGT TCACCTTCGA TCGATACTTC CTCATCAGAA 
AGTAATTCAC TACACCACGT CGTTCCTATC AAAACGTCAG ATACCTGTCC TAAGGAGAAT 
ACAGAGGAAT CTATTATCGC TGATCTTCCA CTCCCCGATC TGCCTCCAGA ACCTCCTACC 
GAATTATCAG ACTCCTTTAA AGAACTCCCA CCGATAAATT CTCATCAAAC TAATTCCAGT 
TTGGGTGGTA TTGGTGACTC TAATGCCTAT ACTACTATCA ACAGTAAGAA AAGATCATTA 
GAAGATAATG AAACTGAAAT TAAGGTATCA CGAGACACAT GGAATACTAA GAATATGCGT 
AGTTTAGAAC CTCCGAGATC GAAGAAACGA ATTCACCTGA TTGCAGCTGT AAAAGCAGTA 
AAATCAATCA AACCAATACG AACAACCTTA CGATACGATG AGGCAATCAC CTATAATAAA 
GATATTAAAG AAAAAGAAAA ATATATCCAG GCATACCACA AAGAAGTCAA TCAACTGTTG 
ATGATGAAAA CTTGGGACAC TGACAGATAT TATGACAGAA AAGAAATAGA CCCCAAAAGA 
GTAATAAATT CAATGTTTAT CTTTAACAGG AAACGTGACG GTACTCATAA AGCTAGATTT 
GTTGCAAGAG GTGATATTCA ACATCCTGAC ACTTACGACC CAGGCATGCA ATCCAATACC 
GTACATCACT ATGCATTAAT GACATCCCTG TCACTTGCAT TAGACAATAA CTACTATATT 
ACACAATTAG ACATATCTTC GGCATATTTG TATGCAGACA TCAAAGAAGA ACTATACATA 
AGACCTCCAC CACATTTAGG AATGAATGAT AAGTTGATAC GTTTGAAGAA ATCACTTTAT 
GGATTGAAAC AAAGTGGAGC GAACTGGTAC GAAACTATCA AATCATACCT GATAAAACAG 
TGTGGTATGG AAGAAGTTCG TGGATGGTCA TGCGTATTTA AGAATAGTCA AGTAACAATT 
TGCTTATTCG TTGATGATAT GATATTGTTC AGCAAAGACT TAAATGCAAA TAAGAAAATC 
ATAACAACAC TCAAGAAACA ATACGATACA AAGATAATAA ATCTGGGTGA AAGTGATAAC 
GAAATTCAGT ACGACATACT TGGCTTAGAA ATCAAATATC AAAGAGGTAA ATACATGAAA 
TTAGGTATGG AAAACTCATT AACTGAGAAA ATACCCAAAT TAAACGTACC TTTGAATCCA 
AAAGGAAGAA AACTTAGCGC TCCAGGTCAA CCAGGTCTTT ATATAGACCA GGATGAACTA 
GAAATAGATG AAGATGAATA CAAAGAGAAG GTACATGAAA TGCAAAAGTT GATTGGTCTA 
GCTTCATATG TTGGATATAA ATTTAGATTT GACTTACTAT ACTACATCAA CACACTTGCT 
CAACATATAC TATTCCCCTC TAGGCAAGTT TTAGACATGA CATATGAGTT AATACAATTC 
ATGTGGGACA CTAGAGATAA ACAACTGATA TGGCACAAAA ACAAACCTAC CGAGCCAGAT 
AATAAACTAG TCGCAATAAG TGATGCTTCA TATGGTAACC AACCATATTA CAAGTCACAA 
ATTGGCAACA TATATTTACT TAATGGAAAG GTAATTGGAG GAAAGTCCAC CAAGGCTTCA 
TTAACATGTA CTTCAACTAC GGAAGCAGAA ATACACGCGA TAAGTGAATC TGTCCCATTA 
TTAAATAACC TCAGTCACCT TGTGCAAGAA CTTAACAAGA AACCAATTAC TAAAGGATTA 
CTAACCGACA GTAAATCTAC AATCAGTATA ATTATATCCA ATAATGAAGA GAAATTTAGA 
AACAGATTTT TTGGTACTAA AGCAATGAGA CTAAGAGATG AAGTATCAGG AAATCATCTG 
CACGTATGCT ATATCGAAAC CAAAAAGAAT ATTGCAGACG TAATGACCAA ACCTCTTCCG 
ATAAAAACAT TCAAACTATT AACAAACAAA TGGATTCATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012086.1
CDS	join(1..1416,1418..5383)
Translation	MESQQLSNYPHISHGSACASVTSKEVHTNQDPLDVSASKIQEYDKASTKANSQQTTTPASSAVPENLHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWSFYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADSDMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELTFLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQYNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNMTVAELFLDIHAIYEEQQGSRNSKPNYRRNPSDEKNDSRSYTNTTKPKVIARNPQKTNNSKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLGQKLTESTVNHTNHSDDELPGHLLLDSGASRTLIRSAHHIHSASSNPDINVVDAQKRNIPINAIGDLQFHFQDNTKTSIKVLHTPNIAYDLLSLNELAAVDITACFTKNVLERSDGTVLAPIVKYGDFYWVSKKYLLPSNISVPTINNVHTSESTRKYPYPFIHRMLAHANAQTIRYSLKNNTITYFNESDVDWSSAIDYQCPDCLIGKSTKHRHIKGSRLKYQNSYEPFQYLHTDIFGPVHNLPKSAPSYFISFTDETTKFRWVYPLHDRREDSILDVFTTILAFIKNQFQASVLVIQMDRGSEYTNRTLHKFLEKNGITPCYTTTADSRAHGVAERLNRTLLDDCRTQLQCSGLPNHLWFSAIEFSTIVRNSLASPKSKKSARQHAGLAGLDISTLLPFGQPVIVNDHNPNSKIHPRGIPGYALHPSRNSYGYIIYLPSLKKTVDTTNYVILQGKESRLDQFNYDALTFDEDLNRLTASYHSFIASNEIQQSNDLNIESDHDFQSDIELHPEQLRNVLSKAVSPTDSTPPSTHTEDSKRVSKTNIRAPREVDPNISESNILPSKKRSSTPQISDIESTGSGGMHRLDVPLLAPMSQSNTHESSHASKSKDFRHSDSYSDNETNHTNVPISSTGGTNNKTVPQTSEQETEKRIIHRSPSIDTSSSESNSLHHVVPIKTSDTCPKENTEESIIADLPLPDLPPEPPTELSDSFKELPPINSHQTNSSLGGIGDSNAYTTINSKKRSLEDNETEIKVSRDTWNTKNMRSLEPPRSKKRIHLIAAVKAVKSIKPIRTTLRYDEAITYNKDIKEKEKYIQAYHKEVNQLLMMKTWDTDRYYDRKEIDPKRVINSMFIFNRKRDGTHKARFVARGDIQHPDTYDPGMQSNTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIKEELYIRPPPHLGMNDKLIRLKKSLYGLKQSGANWYETIKSYLIKQCGMEEVRGWSCVFKNSQVTICLFVDDMILFSKDLNANKKIITTLKKQYDTKIINLGESDNEIQYDILGLEIKYQRGKYMKLGMENSLTEKIPKLNVPLNPKGRKLSAPGQPGLYIDQDELEIDEDEYKEKVHEMQKLIGLASYVGYKFRFDLLYYINTLAQHILFPSRQVLDMTYELIQFMWDTRDKQLIWHKNKPTEPDNKLVAISDASYGNQPYYKSQIGNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAISESVPLLNNLSHLVQELNKKPITKGLLTDSKSTISIIISNNEEKFRNRFFGTKAMRLRDEVSGNHLHVCYIETKKNIADVMTKPLPIKTFKLLTNKWIH

Target ORF information:

RefSeq Version	NM_001181431.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C gag-pol fusion protein (YHR214C-B), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181431.2

ORF Insert Sequence:

ATGGAATCCC AACAATTATC TAATTACCCA CATATATCTC ATGGTAGCGC CTGTGCTTCG 
GTTACTTCTA AGGAAGTCCA CACAAATCAA GATCCGTTAG ACGTTTCAGC TTCCAAAATT 
CAAGAATATG ATAAGGCTTC CACTAAGGCT AACTCTCAAC AGACAACAAC ACCTGCTTCA 
TCAGCTGTTC CAGAGAACCT CCATCATGCC TCTCCTCAAC CTGCTTCAGT ACCACCTCCA 
CAGAATGGGC CGTACCCACA GCAGTGCATG ATGACCCAAA ACCAAGCCAA TCCATCTGGT 
TGGTCATTTT ACGGACACCC ATCTATGATT CCGTATACAC CTTATCAAAT GTCGCCTATG 
TACTTTCCAC CTGGGCCACA ATCACAGTTT CCGCAGTATC CATCATCAGT TGGAACGCCT 
CTGAGCACTC CATCACCTGA GTCAGGTAAT ACATTTACTG ATTCATCCTC AGCGGACTCT 
GATATGACAT CCACTAAAAA ATATGTCAGA CCACCACCAA TGTTAACCTC ACCTAATGAC 
TTTCCAAATT GGGTTAAAAC ATACATCAAA TTTTTACAAA ACTCGAATCT CGGTGGTATT 
ATTCCGACAG TAAACGGAAA ACCCGTACCA CCAATGTTAA CCTCACCTAA TGACTTTCCA 
AATTGGGTTA AAACATACAT CAAATTTTTA CAAAACTCGA ATCTCGGTGG TATTATTCCG 
ACAGTAAACG GAAAACCCGT ACGTCAGATC ACTGATGATG AACTCACCTT CTTGTATAAC 
ACTTTTCAAA TATTTGCTCC CTCTCAATTC CTACCTACCT GGGTCAAAGA CATCCTATCC 
GTTGATTATA CGGATATCAT GAAAATTCTT TCCAAAAGTA TTGAAAAAAT GCAATCTGAT 
ACCCAAGAGG CAAACGACAT TGTGACCCTG GCAAATTTGC AATATAATGG CAGTACACCT 
GCAGATGCAT TTGAAACAAA AGTCACAAAC ATTATCGACA GACTGAACAA TAATGGCATT 
CATATCAATA ACAAGGTCGC ATGCCAATTA ATTATGAGAG GTCTATCTGG CGAATATAAA 
TTTTTACGCT ACACACGTCA TCGACATCTA AATATGACAG TCGCTGAACT GTTCTTAGAT 
ATCCATGCTA TTTATGAAGA ACAACAGGGA TCGAGAAACA GCAAACCTAA TTACAGGAGA 
AATCCGAGTG ATGAGAAGAA TGATTCTCGC AGCTATACGA ATACAACCAA ACCCAAAGTT 
ATAGCTCGGA ATCCTCAAAA AACAAATAAT TCGAAATCGA AAACAGCCAG GGCTCACAAT 
GTATCCACAT CTAATAACTC TCCCAGCACG GACAACGATT CCATCAGTAA ATCAACTACT 
GAACCGATTC AATTGAACAA TAAGCACGAC CTTCATTTAG GCCAGAAACT TACTGAATCT 
ACAGTAAATC ATACTAATCA TTCTGATGAT GAACTCCCTG GACACCTCCT TCTCGATTCA 
GGAGCATCAC GAACCCTTAT AAGATCTGCT CATCACATAC ACTCAGCATC ATCTAATCCT 
GACATAAACG TAGTTGATGC TCAAAAAAGA AATATACCAA TTAACGCTAT TGGTGACCTA 
CAATTTCACT TCCAGGACAA CACCAAAACA TCAATAAAGG TATTGCACAC TCCTAACATA 
GCCTATGACT TACTCAGTTT GAATGAATTG GCTGCAGTAG ATATCACAGC ATGCTTTACC 
AAAAACGTCT TAGAACGGTC TGACGGCACT GTACTTGCAC CTATCGTAAA ATATGGAGAC 
TTTTACTGGG TATCTAAAAA GTACTTGCTT CCATCAAATA TCTCCGTACC CACCATCAAT 
AATGTCCATA CAAGTGAAAG TACACGCAAA TATCCTTATC CTTTCATTCA TCGAATGCTT 
GCGCATGCCA ATGCACAGAC AATTCGATAC TCACTTAAAA ATAACACCAT CACGTATTTT 
AACGAATCAG ATGTCGACTG GTCTAGTGCT ATTGACTATC AATGTCCTGA TTGTTTAATC 
GGCAAAAGCA CCAAACACAG ACATATCAAA GGTTCACGAC TAAAATACCA AAATTCATAC 
GAACCCTTTC AATACCTACA TACTGACATA TTTGGTCCAG TTCACAACCT ACCAAAAAGT 
GCACCATCCT ATTTCATCTC ATTTACTGAT GAGACAACAA AATTCCGTTG GGTTTATCCA 
TTACACGACC GTCGCGAGGA CTCTATCCTC GATGTTTTTA CTACGATACT AGCTTTTATT 
AAAAACCAGT TTCAGGCCAG TGTCTTGGTT ATACAAATGG ACCGTGGTTC TGAGTATACT 
AACAGAACTC TCCATAAATT CCTTGAAAAA AATGGTATAA CTCCATGCTA TACAACCACA 
GCGGATTCCC GAGCACATGG AGTCGCTGAA CGGCTCAACC GTACCTTATT AGATGACTGC 
CGTACTCAAC TGCAATGTAG TGGTTTACCG AACCATTTAT GGTTCTCTGC AATCGAATTT 
TCTACTATTG TGAGAAATTC ACTAGCTTCA CCTAAAAGCA AAAAATCTGC AAGACAACAT 
GCTGGCTTGG CAGGACTTGA TATCAGTACT TTGTTACCTT TCGGTCAACC TGTTATCGTC 
AATGATCACA ACCCTAACTC CAAAATACAT CCTCGTGGCA TCCCAGGCTA CGCTCTACAT 
CCGTCTCGAA ACTCTTATGG ATATATCATC TATCTTCCGT CCTTAAAGAA GACAGTAGAT 
ACAACTAACT ATGTTATTCT TCAGGGCAAG GAATCCAGAT TAGATCAATT CAATTACGAC 
GCACTCACTT TCGATGAAGA CTTAAACCGT TTAACTGCTT CATATCATTC GTTCATTGCG 
TCAAATGAGA TCCAACAATC CAATGATCTT AACATAGAAT CTGACCATGA CTTCCAATCT 
GACATTGAAC TACATCCTGA GCAACTGAGA AATGTCCTTT CAAAAGCTGT GAGTCCAACC 
GATTCCACAC CTCCGTCAAC TCATACTGAA GATTCGAAAC GTGTTTCCAA AACCAATATT 
CGCGCACCCA GAGAAGTTGA CCCCAACATA TCTGAATCTA ATATTCTTCC ATCTAAGAAG 
AGATCTAGCA CCCCCCAAAT TTCCGATATC GAGAGTACCG GTTCGGGTGG TATGCATAGA 
TTAGATGTTC CTTTACTTGC TCCCATGTCC CAATCTAACA CACATGAGTC GTCGCACGCC 
AGTAAATCTA AAGATTTCAG ACACTCAGAC TCGTACAGTG ACAATGAGAC TAATCATACA 
AACGTACCAA TATCCAGTAC GGGTGGTACC AATAATAAAA CTGTTCCGCA GACAAGCGAA 
CAGGAGACTG AGAAAAGGAT CATACACCGT TCACCTTCGA TCGATACTTC CTCATCAGAA 
AGTAATTCAC TACACCACGT CGTTCCTATC AAAACGTCAG ATACCTGTCC TAAGGAGAAT 
ACAGAGGAAT CTATTATCGC TGATCTTCCA CTCCCCGATC TGCCTCCAGA ACCTCCTACC 
GAATTATCAG ACTCCTTTAA AGAACTCCCA CCGATAAATT CTCATCAAAC TAATTCCAGT 
TTGGGTGGTA TTGGTGACTC TAATGCCTAT ACTACTATCA ACAGTAAGAA AAGATCATTA 
GAAGATAATG AAACTGAAAT TAAGGTATCA CGAGACACAT GGAATACTAA GAATATGCGT 
AGTTTAGAAC CTCCGAGATC GAAGAAACGA ATTCACCTGA TTGCAGCTGT AAAAGCAGTA 
AAATCAATCA AACCAATACG AACAACCTTA CGATACGATG AGGCAATCAC CTATAATAAA 
GATATTAAAG AAAAAGAAAA ATATATCCAG GCATACCACA AAGAAGTCAA TCAACTGTTG 
ATGATGAAAA CTTGGGACAC TGACAGATAT TATGACAGAA AAGAAATAGA CCCCAAAAGA 
GTAATAAATT CAATGTTTAT CTTTAACAGG AAACGTGACG GTACTCATAA AGCTAGATTT 
GTTGCAAGAG GTGATATTCA ACATCCTGAC ACTTACGACC CAGGCATGCA ATCCAATACC 
GTACATCACT ATGCATTAAT GACATCCCTG TCACTTGCAT TAGACAATAA CTACTATATT 
ACACAATTAG ACATATCTTC GGCATATTTG TATGCAGACA TCAAAGAAGA ACTATACATA 
AGACCTCCAC CACATTTAGG AATGAATGAT AAGTTGATAC GTTTGAAGAA ATCACTTTAT 
GGATTGAAAC AAAGTGGAGC GAACTGGTAC GAAACTATCA AATCATACCT GATAAAACAG 
TGTGGTATGG AAGAAGTTCG TGGATGGTCA TGCGTATTTA AGAATAGTCA AGTAACAATT 
TGCTTATTCG TTGATGATAT GATATTGTTC AGCAAAGACT TAAATGCAAA TAAGAAAATC 
ATAACAACAC TCAAGAAACA ATACGATACA AAGATAATAA ATCTGGGTGA AAGTGATAAC 
GAAATTCAGT ACGACATACT TGGCTTAGAA ATCAAATATC AAAGAGGTAA ATACATGAAA 
TTAGGTATGG AAAACTCATT AACTGAGAAA ATACCCAAAT TAAACGTACC TTTGAATCCA 
AAAGGAAGAA AACTTAGCGC TCCAGGTCAA CCAGGTCTTT ATATAGACCA GGATGAACTA 
GAAATAGATG AAGATGAATA CAAAGAGAAG GTACATGAAA TGCAAAAGTT GATTGGTCTA 
GCTTCATATG TTGGATATAA ATTTAGATTT GACTTACTAT ACTACATCAA CACACTTGCT 
CAACATATAC TATTCCCCTC TAGGCAAGTT TTAGACATGA CATATGAGTT AATACAATTC 
ATGTGGGACA CTAGAGATAA ACAACTGATA TGGCACAAAA ACAAACCTAC CGAGCCAGAT 
AATAAACTAG TCGCAATAAG TGATGCTTCA TATGGTAACC AACCATATTA CAAGTCACAA 
ATTGGCAACA TATATTTACT TAATGGAAAG GTAATTGGAG GAAAGTCCAC CAAGGCTTCA 
TTAACATGTA CTTCAACTAC GGAAGCAGAA ATACACGCGA TAAGTGAATC TGTCCCATTA 
TTAAATAACC TCAGTCACCT TGTGCAAGAA CTTAACAAGA AACCAATTAC TAAAGGATTA 
CTAACCGACA GTAAATCTAC AATCAGTATA ATTATATCCA ATAATGAAGA GAAATTTAGA 
AACAGATTTT TTGGTACTAA AGCAATGAGA CTAAGAGATG AAGTATCAGG AAATCATCTG 
CACGTATGCT ATATCGAAAC CAAAAAGAAT ATTGCAGACG TAATGACCAA ACCTCTTCCG 
ATAAAAACAT TCAAACTATT AACAAACAAA TGGATTCATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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YHR214C-B cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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