YHL009W-B cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	YHL009W-B
Entrez Gene ID	856380
Full Name	gag-pol fusion protein
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001184404.2	NP_058133.1	gag-pol fusion protein

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	YNL054W-B	NP_001268465.1
Saccharomyces cerevisiae (baker's yeast)	YPR137C-A	NP_058192.1
Saccharomyces cerevisiae (baker's yeast)	YDR098C-B	NP_058140.1
Saccharomyces cerevisiae (baker's yeast)	YDR170W-A	NP_010455.1
Saccharomyces cerevisiae (baker's yeast)	YML039W	NP_013672.1
Saccharomyces cerevisiae (baker's yeast)	YHR214C-B	NP_012086.1
Saccharomyces cerevisiae (baker's yeast)	YML045W	NP_013668.1
Saccharomyces cerevisiae (baker's yeast)	YML040W	NP_013673.1
Saccharomyces cerevisiae (baker's yeast)	YMR050C	NP_013765.1
Saccharomyces cerevisiae (baker's yeast)	YPR158W-A	NP_058194.1
Saccharomyces cerevisiae (baker's yeast)	YGR161W-A	NP_058164.1
Saccharomyces cerevisiae (baker's yeast)	YLR410W-A	NP_058175.1
Saccharomyces cerevisiae (baker's yeast)	YER137C-A	NP_058155.1
Saccharomyces cerevisiae (baker's yeast)	YHR214C-C	NP_036195.3
Saccharomyces cerevisiae (baker's yeast)	YPR137C-B	NP_058191.1
Saccharomyces cerevisiae (baker's yeast)	YDR261W-A	NP_058147.3
Saccharomyces cerevisiae (baker's yeast)	YGR038C-B	NP_058160.1
Saccharomyces cerevisiae (baker's yeast)	YBL005W-B	NP_009549.1
Saccharomyces cerevisiae (baker's yeast)	YMR045C	NP_013759.1
Saccharomyces cerevisiae (baker's yeast)	YBR012W-B	NP_009567.1
Saccharomyces cerevisiae (baker's yeast)	YDR210W-B	NP_058142.3
Saccharomyces cerevisiae (baker's yeast)	YDR210C-D	NP_620387.1
Saccharomyces cerevisiae (baker's yeast)	YGR161W-B	NP_058163.1
Saccharomyces cerevisiae (baker's yeast)	YER160C	NP_011087.3
Saccharomyces cerevisiae (baker's yeast)	YGR027W-B	NP_058158.1
Saccharomyces cerevisiae (baker's yeast)	YLR157C-B	NP_058169.1
Saccharomyces cerevisiae (baker's yeast)	YLR256W-A	NP_058173.1
Saccharomyces cerevisiae (baker's yeast)	YBR012W-A	NP_009568.1
Saccharomyces cerevisiae (baker's yeast)	YCL020W	NP_009910.2
Saccharomyces cerevisiae (baker's yeast)	YMR051C	NP_013766.1
Saccharomyces cerevisiae (baker's yeast)	YDR210W-A	NP_058143.1
Saccharomyces cerevisiae (baker's yeast)	YLR227W-A	NP_058172.1
Saccharomyces cerevisiae (baker's yeast)	YGR027W-A	NP_058159.1
Saccharomyces cerevisiae (baker's yeast)	YML045W-A	NP_058176.1
Saccharomyces cerevisiae (baker's yeast)	YPR158C-C	NP_058196.1
Saccharomyces cerevisiae (baker's yeast)	YBL100W-B	NP_009450.1
Saccharomyces cerevisiae (baker's yeast)	YOR343W-B	NP_620389.3
Saccharomyces cerevisiae (baker's yeast)	YDR365W-B	NP_058152.1
Saccharomyces cerevisiae (baker's yeast)	YGR161C-D	NP_058165.3
Saccharomyces cerevisiae (baker's yeast)	YOR192C-B	NP_058185.3
Saccharomyces cerevisiae (baker's yeast)	YLR035C-A	NP_013136.1
Saccharomyces cerevisiae (baker's yeast)	YJL113W	NP_012421.2
Saccharomyces cerevisiae (baker's yeast)	YLR227W-B	NP_058171.1
Saccharomyces cerevisiae (baker's yeast)	YPR158C-D	NP_058195.1
Saccharomyces cerevisiae (baker's yeast)	YPR158W-B	NP_058193.1
Saccharomyces cerevisiae (baker's yeast)	YJR028W	NP_012563.1
Saccharomyces cerevisiae (baker's yeast)	YDR210C-C	NP_620388.3
Saccharomyces cerevisiae (baker's yeast)	YPL257W-A	NP_058190.1
Saccharomyces cerevisiae (baker's yeast)	YDR365W-A	NP_058153.3
Saccharomyces cerevisiae (baker's yeast)	YFL002W-B	NP_058157.1
Saccharomyces cerevisiae (baker's yeast)	YAR009C	NP_009406.1
Saccharomyces cerevisiae (baker's yeast)	YBL100W-A	NP_009451.1
Saccharomyces cerevisiae (baker's yeast)	YPL257W-B	NP_058189.1
Saccharomyces cerevisiae (baker's yeast)	YFL002W-A	NP_116653.1
Saccharomyces cerevisiae (baker's yeast)	YJR029W	NP_012562.1
Saccharomyces cerevisiae (baker's yeast)	YJR027W	NP_012560.1
Saccharomyces cerevisiae (baker's yeast)	YHL009W-B	NP_058133.1
Saccharomyces cerevisiae (baker's yeast)	YNL284C-B	NP_058178.1
Saccharomyces cerevisiae (baker's yeast)	YNL054W-A	NP_058180.1
Saccharomyces cerevisiae (baker's yeast)	YBL005W-A	NP_009550.1
Saccharomyces cerevisiae (baker's yeast)	YNL284C-A	NP_058179.1
Saccharomyces cerevisiae (baker's yeast)	YDR316W-A	NP_058151.1
Saccharomyces cerevisiae (baker's yeast)	YER159C-A	NP_058156.1
Saccharomyces cerevisiae (baker's yeast)	YLR157C-A	NP_058170.1
Saccharomyces cerevisiae (baker's yeast)	YJR026W	NP_012561.3
Saccharomyces cerevisiae (baker's yeast)	YAR010C	NP_009407.1
Saccharomyces cerevisiae (baker's yeast)	YDR034C-D	NP_058138.3
Saccharomyces cerevisiae (baker's yeast)	YLR410W-B	NP_058174.3
Saccharomyces cerevisiae (baker's yeast)	YCL019W	NP_009909.2
Saccharomyces cerevisiae (baker's yeast)	YMR046C	NP_013760.1
Saccharomyces cerevisiae (baker's yeast)	YOR142W-B	NP_058183.1
Saccharomyces cerevisiae (baker's yeast)	YDR316W-B	NP_058150.1
Saccharomyces cerevisiae (baker's yeast)	YDR261W-B	NP_058146.3
Saccharomyces cerevisiae (baker's yeast)	YDR261C-D	NP_058148.1
Saccharomyces cerevisiae (baker's yeast)	YER138C	NP_011064.3
Saccharomyces cerevisiae (baker's yeast)	YOL103W-B	NP_058181.1
Saccharomyces cerevisiae (baker's yeast)	YDR034C-C	NP_058139.1
Saccharomyces cerevisiae (baker's yeast)	YDR098C-A	NP_058141.1
Saccharomyces cerevisiae (baker's yeast)	YOR343W-A	NP_620390.1
Saccharomyces cerevisiae (baker's yeast)	YGR161C-C	NP_058166.1
Saccharomyces cerevisiae (baker's yeast)	YDR261C-C	NP_058149.1
Saccharomyces cerevisiae (baker's yeast)	YOR192C-A	NP_058186.1
Saccharomyces cerevisiae (baker's yeast)	YGR038C-A	NP_058161.1
Saccharomyces cerevisiae (baker's yeast)	YOR142W-A	NP_058184.3
Saccharomyces cerevisiae (baker's yeast)	YOL103W-A	NP_058182.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following YHL009W-B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the YHL009W-B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi178690	NM_001184404.2 Latest version!	Saccharomyces cerevisiae S288C gag-pol fusion protein (YHL009W-B), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

YHL009W-B ( NM_001184404.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_058133.1 Saccharomyces cerevisiae S288C gag-pol fusion protein (YHL009W-B), partial mRNA.

Price & Availability↑

CloneID	OSi178690
Clone ID Related Accession (Same CDS sequence)	NM_001184404.2
Accession Version	NM_001184404.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	gag-pol fusion protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). On Feb 18, 2015 this sequence version replaced NM_001184404.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCGACCC CAGTGAGGGA TGAAACAAGA AATGTTATTG ACGACAACAT TTCTGCGCGG 
ATTCAATCGA AAGTCAAAAC AAATGATACT GTCAGACAGA CGCCATCATC ATTAAGAAAA 
GTTTCTATTA AAGATGAACA GGTGAAACAA TATCAAAGAA ACTTAAATAG GTTTAAAACC 
ATACTAAATG GTTTAAAGGC AGAAGAGGAA AAACTTTCTG AGACTGATGA TATTCAGATG 
CTAGCTGAAA AATTATTAAA ACTCGGAGAA ACCATTGACA AGGTTGAGAA TAGGATTGTG 
GATCTAGTTG AAAAGATACA ATTATTGGAA ACAAACGAGA ACAATAATAT ATTACATGAA 
CATATAGATG CTACAGGGAC TTACTATTTA TTCGATACGT TAACTTCAAC CAACAAAAGA 
TTCTACCCTA AGGATTGTGT TTTTGATTAT AGGACTAATA ATGTCGAGAA CATTCCTATT 
CTCTTAAACA ATTTTAAAAA ATTCATCAAG AAATATCAAT TTGATGATGT CTTTGAAAAT 
GATATCATAG AAATCGATCC TCGTGAAAAT GAAATCTTGT GCAAGATAAT CAAAGAAGGA 
CTCGGTGAAA GTTTAGATAT CATGAACACA AATACAACTG ACATTTTTAG GATAATCGAT 
GGTTTAAAAA ACAAATATAG AAGTTTACAT GGTAGAGATG TCAGAATTAG AGCCTGGGAA 
AAGGTTTTGG TTGATACAAC ATGTAGAAAT TCCGCATTGT TAATGAATAA ACTTCAAAAG 
TTGGTACTAA TGGAAAAATG GATTTTTTCT AAATGCTGCC AAGATTGTCC TAATCTAAAG 
GATTACCTAC AAGAAGCTAT CATGGGAACC TTACATGAAT CCTTAAGAAA TTCTGTGAAA 
CAACGTTTGT ACAACATTCC ACATAACGTA GGAATTAATC ACGAAGAATT TCTAATCAAT 
ACTGTTATTG AAACAGTAAT TGATTTGAGC CCAATTGCAG ACGATCAAAT AGAAAATAGC 
TGCATGTATT GCAAATCTGT TTTCCATTGC TCAATTAACT GCAAAAAGAA ACCAAATAGG 
GAACTTGGCC TGACTCGACC AATTTCTCAA AAACCTATTA TCTACAAGGT GCACAGAGAC 
AACAACAACT TAAGTCCAGT GCAAAACGAA CAAAAGTCTT GGAACAAGAC ACAAAAAAAG 
TCAAACAAAG TGTACAACAG CAAAAAACTG GTAATTATTG ATACCGGTTC CGGCGTAAAC 
ATTACCAATG ACAAAACCTT ACTGCATAAT TACGAAGACA GTAATCGCAG TACACGATTT 
TTTGGTATTG GGAAAAACAG TTCAGTGTCT GTTAAAGGGT ATGGCTATAT AAAAATCAAG 
AATGGTCACA ACAATACTGA CAATAAGTGT TTATTGACTT ACTATGTACC GGAAGAAGAA 
TCCACTATAA TCAGCTGTTA TGACTTAGCC AAGAAAACCA AAATGGTTTT AAGTCGAAAA 
TATACCAGAT TGGGAAACAA AATCATAAAA ATTAAAACCA AGATAGTTAA TGGTGTCATT 
CACGTAAAAA TGAACGAGTT AATTGAACGT CCTTCCGATG ATTCAAAAAT AAATGCAATA 
AAACCTACTT CTTCTCCTGG ATTTAAACTA AATAAAAGGT CTATTACCTT GGAAGATGCT 
CATAAAAGAA TGGGCCATAC AGGAATTCAA CAAATTGAAA ATTCCATAAA ACATAATCAT 
TATGAAGAAT CCCTTGACTT AATCAAAGAA CCAAATGAAT TTTGGTGTCA AACCTGTAAA 
ATCTCTAAAG CCACGAAAAG AAATCATTAT ACCGGGTCTA TGAATAATCA TAGTACTGAT 
CATGAACCAG GATCATCATG GTGCATGGAT ATATTTGGCC CTGTATCAAG TTCAAACGCG 
GACACTAAAA GGTACATGCT TATTATGGTG GATAACAACA CGAGATATTG CATGACCTCC 
ACACACTTCA ATAAGAATGC TGAAACTATT TTAGCTCAAA TCAGAAAGAA TATTCAGTAC 
GTGGAAACAC AATTTGACAG GAAAGTCAGA GAAATTAATT CAGACAGAGG TACTGAATTC 
ACAAATGATC AGATAGAAGA ATATTTTATT TCAAAAGGAA TACATCACAT ACTTACTTCT 
ACACAAGATC ATGCTGCTAA TGGAAGAGCA GAAAGATACA TCAGAACAAT AGTAACTGAT 
GCAACAACAC TCCTAAGACA AAGTAACTTA AGAGTAAAAT TTTGGGAATA CGCAGTAACT 
TCTGCTACCA ATATAAGAAA TTGCCTGGAA CACAAAAGTA CAGGTAAACT ACCATTGAAG 
GCAATCTCAC GTCAACCTGT TACAGTGAGA TTAATGTCAT TCTTACCATT TGGCGAAAAA 
GGAATAATTT GGAATCATAA TCACAAAAAA TTGAAACCAT CTGGACTTCC TTCTATAATT 
CTATGCAAAG ATCCAAATAG TTATGGATAC AAATTCTTTA TACCATCCAA AAATAAAATT 
GTCACATCTG ATAATTATAC AATTCCCAAC TATACGATGG ACGGTAGAGT AAGAAATACT 
CAGAATATTT ACAAGAGTCA TCAATTCAGT TCACATAATG ATAATGAAGA AGATCAAATC 
GAAACGGTCA CAAACTTATG TGAAGCTTTG GAAAACTACG AAGATGATAA TAAACCAATT 
ACTCGCCTGG AAGATTTGTT CACAGAGGAA GAGTTATCTC AAATAGACTC AAACGCAAAA 
TACCCATCTC CTAGTAATAA CCTAGAAGGG GACTTGGATT ACGTATTTTC TGATGTTGAG 
GAATCTGGAG ATTATGACGT TGAATCTGAA CTTTCAACGA CAAATACTTC AATCTCAACT 
GATAAAAACA AAATTTTGTC AAACAAGGAT TTTAATTCAG AACTTGCATC GACTGAAATA 
TCCATCAGTG AAATCGATAA GAAAGGATTA ATAAATACAA GTCATATTGA TGAAGATAAG 
TATGATGAAA AAGTCCACAG AATTCCATCG ATTATACAAG AGAAACTGGT AGGAAGTAAA 
AATACTATTA AAATCAATGA CGAAAACAGA ATCTCCGACA GAATTCGTAG TAAAAATATT 
GGGAGTATCT TAAACACTGG ACTCAGTAGA TGTGTAGATA TCACCGATGA ATCTATTACT 
AACAAAGATG AGTCAATGCA CAACGCAAAA CCCGAACTAA TTCAGGAGCA GTTCAATAAA 
ACAAATCATG AAACTTCGTT TCCTAAAGAA GGGAGCATTG GAACAAATGT AAAATTCCGA 
AATACAGACA ATGAGATTTC TTTAAAAACA GGCGATACGA GTTTACCAAT AAAAACTTTA 
GAAAGCATTA ACAATCACCA TAGTAATGAT TATTCCACAA ACAAAGTTGA AAAGTTTGAG 
AAGGAAAATC ATCATCCGCC CCCGATTGAG GACATTGTGG ATATGAGTGA TCAAACTGAT 
ATGGAATCAA ACTGTCAGGA TGGTAATAAC TTAAAAGAAT TAAAAGTCAC CGATAAAAAT 
GTACCAACTG ACAATGGAAC AAATGTGTCA CCAAGGTTGG AACAAAATAT TGAAGCATCT 
GGATCACCAG TACAAACAGT TAATAAAAGT GCCTTCTTAA ACAAAGAATT CAGTTCTTTG 
AACATGAAAA GAAAACGGAA AAGACACGAT AAAAACAATA GTCTAACAAG CTATGAATTA 
GAAAGAGATA AGAAGCGTTC AAAAAGGAAT CGAGTGAAAT TAATTCCAGA TAATATGGAA 
ACAGTTTCAG CACAAAAAAT TAGAGCCATA TATTATAATG AAGCTATTTC AAAAAATCCT 
GACCTCAAAG AAAAACATGA ATACAAACAG GCATATCATA AAGAATTACA GAATTTAAAA 
GATATGAAGG TATTTGATGT CGATGTGAAG TACAGTAGAT CAGAAATCCC TGATAATTTA 
ATAGTACCCA CCAACACGAT ATTCACAAAG AAAAGAAATG GGATTTATAA GGCTAGGATA 
GTCTGCAGAG GTGATACTCA GTCACCAGAC ACTTACAGTG TAATAACTAC AGAATCTTTA 
AATCACAATC ATATTAAGAT ATTCTTAATG ATTGCAAACA ACAGAAATAT GTTTATGAAG 
ACCCTGGATA TCAATCATGC ATTCCTATAT GCTAAATTGG AAGAAGAAAT ATACATCCCA 
CATCCGCATG ATAGGAGATG TGTAGTCAAG CTAAATAAGG CGTTATATGG TCTAAAACAG 
AGTCCTAAAG AATGGAATGA TCATCTAAGA CAATACTTGA ATGGAATTGG ACTGAAAGAT 
AACTCTTATA CTCCGGGATT ATACCAAACC GAGGATAAAA ATCTAATGAT TGCAGTCTAT 
GTTGATGACT GCGTAATTGC GGCAAGCAAT GAACAGAGAT TGGATGAATT CATAAACAAA 
TTGAAAAGTA ATTTTGAACT GAAAATTACA GGAACATTAA TAGATGATGT ACTCGATACA 
GATATATTAG GAATGGATCT AGTATACAAC AAAAGACTTG GTACTATCGA TTTAACATTA 
AAATCATTCA TAAATAGAAT GGATAAAAAA TACAACGAGG AATTGAAAAA GATTAGAAAA 
AGTTCAATTC CGCATATGTC AACTTATAAA ATAGATCCTA AGAAAGACGT ACTGCAAATG 
TCAGAAGAAG AGTTTAGACA AGGTGTTCTA AAGCTACAAC AATTACTAGG TGAACTAAAC 
TATGTCAGAC ACAAATGCAG ATACGACATT AATTTTGCTG TTAAGAAAGT GGCTAGACTA 
GTAAATTACC CACATGAAAG AGTCTTTTAT ATGATTTACA AAATAATCCA GTACTTGGTT 
CGGTATAAAG ATATTGGAAT ACACTATGAC CGAGACTGTA ATAAAGACAA GAAGGTTATT 
GCTATAACTG ATGCATCAGT TGGATCAGAA TATGATGCTC AATCAAGGAT TGGAGTTATA 
TTATGGTACG GTATGAATAT TTTTAATGTT TATTCTAACA AGAGCACAAA CAGATGTGTA 
TCATCAACAG AAGCAGAGCT TCATGCCATT TATGAAGGCT ATGCAGACTC AGAAACGTTG 
AAGGTAACAT TAAAGGAGCT AGGAGAAGGA GACAATAATG ACATTGTCAT GATCACTGAC 
TCAAAGCCAG CCATTCAAGG ATTAAATCGC AGCTATCAAC AACCAAAAGA GAAATTCACT 
TGGATAAAAA CTGAAATAAT AAAAGAAAAA ATTAAAGAGA AGAGTATAAA ACTGTTAAAA 
ATTACCGGCA AAGGTAATAT TGCTGATTTA CTAACAAAAC CAGTATCAGC ATCTGATTTT 
AAAAGATTTA TACAAGTATT AAAAAATAAA ATAACATCAC AGGATATTTT GGCCTCAACA 
GACTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058133.1
CDS	join(1..1086,1088..5410)
Translation	MATPVRDETRNVIDDNISARIQSKVKTNDTVRQTPSSLRKVSIKDEQVKQYQRNLNRFKTILNGLKAEEEKLSETDDIQMLAEKLLKLGETIDKVENRIVDLVEKIQLLETNENNNILHEHIDATGTYYLFDTLTSTNKRFYPKDCVFDYRTNNVENIPILLNNFKKFIKKYQFDDVFENDIIEIDPRENEILCKIIKEGLGESLDIMNTNTTDIFRIIDGLKNKYRSLHGRDVRIRAWEKVLVDTTCRNSALLMNKLQKLVLMEKWIFSKCCQDCPNLKDYLQEAIMGTLHESLRNSVKQRLYNIPHNVGINHEEFLINTVIETVIDLSPIADDQIENSCMYCKSVFHCSINCKKKPNRELGLTRPISQKPIIYKVHRDNNNLSPVQNEQKSWNKTQKKSNKVYNSKKLVIIDTGSGVNITNDKTLLHNYEDSNRSTRFFGIGKNSSVSVKGYGYIKIKNGHNNTDNKCLLTYYVPEEESTIISCYDLAKKTKMVLSRKYTRLGNKIIKIKTKIVNGVIHVKMNELIERPSDDSKINAIKPTSSPGFKLNKRSITLEDAHKRMGHTGIQQIENSIKHNHYEESLDLIKEPNEFWCQTCKISKATKRNHYTGSMNNHSTDHEPGSSWCMDIFGPVSSSNADTKRYMLIMVDNNTRYCMTSTHFNKNAETILAQIRKNIQYVETQFDRKVREINSDRGTEFTNDQIEEYFISKGIHHILTSTQDHAANGRAERYIRTIVTDATTLLRQSNLRVKFWEYAVTSATNIRNCLEHKSTGKLPLKAISRQPVTVRLMSFLPFGEKGIIWNHNHKKLKPSGLPSIILCKDPNSYGYKFFIPSKNKIVTSDNYTIPNYTMDGRVRNTQNIYKSHQFSSHNDNEEDQIETVTNLCEALENYEDDNKPITRLEDLFTEEELSQIDSNAKYPSPSNNLEGDLDYVFSDVEESGDYDVESELSTTNTSISTDKNKILSNKDFNSELASTEISISEIDKKGLINTSHIDEDKYDEKVHRIPSIIQEKLVGSKNTIKINDENRISDRIRSKNIGSILNTGLSRCVDITDESITNKDESMHNAKPELIQEQFNKTNHETSFPKEGSIGTNVKFRNTDNEISLKTGDTSLPIKTLESINNHHSNDYSTNKVEKFEKENHHPPPIEDIVDMSDQTDMESNCQDGNNLKELKVTDKNVPTDNGTNVSPRLEQNIEASGSPVQTVNKSAFLNKEFSSLNMKRKRKRHDKNNSLTSYELERDKKRSKRNRVKLIPDNMETVSAQKIRAIYYNEAISKNPDLKEKHEYKQAYHKELQNLKDMKVFDVDVKYSRSEIPDNLIVPTNTIFTKKRNGIYKARIVCRGDTQSPDTYSVITTESLNHNHIKIFLMIANNRNMFMKTLDINHAFLYAKLEEEIYIPHPHDRRCVVKLNKALYGLKQSPKEWNDHLRQYLNGIGLKDNSYTPGLYQTEDKNLMIAVYVDDCVIAASNEQRLDEFINKLKSNFELKITGTLIDDVLDTDILGMDLVYNKRLGTIDLTLKSFINRMDKKYNEELKKIRKSSIPHMSTYKIDPKKDVLQMSEEEFRQGVLKLQQLLGELNYVRHKCRYDINFAVKKVARLVNYPHERVFYMIYKIIQYLVRYKDIGIHYDRDCNKDKKVIAITDASVGSEYDAQSRIGVILWYGMNIFNVYSNKSTNRCVSSTEAELHAIYEGYADSETLKVTLKELGEGDNNDIVMITDSKPAIQGLNRSYQQPKEKFTWIKTEIIKEKIKEKSIKLLKITGKGNIADLLTKPVSASDFKRFIQVLKNKITSQDILASTDY

Target ORF information:

RefSeq Version	NM_001184404.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C gag-pol fusion protein (YHL009W-B), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184404.2

ORF Insert Sequence:

ATGGCGACCC CAGTGAGGGA TGAAACAAGA AATGTTATTG ACGACAACAT TTCTGCGCGG 
ATTCAATCGA AAGTCAAAAC AAATGATACT GTCAGACAGA CGCCATCATC ATTAAGAAAA 
GTTTCTATTA AAGATGAACA GGTGAAACAA TATCAAAGAA ACTTAAATAG GTTTAAAACC 
ATACTAAATG GTTTAAAGGC AGAAGAGGAA AAACTTTCTG AGACTGATGA TATTCAGATG 
CTAGCTGAAA AATTATTAAA ACTCGGAGAA ACCATTGACA AGGTTGAGAA TAGGATTGTG 
GATCTAGTTG AAAAGATACA ATTATTGGAA ACAAACGAGA ACAATAATAT ATTACATGAA 
CATATAGATG CTACAGGGAC TTACTATTTA TTCGATACGT TAACTTCAAC CAACAAAAGA 
TTCTACCCTA AGGATTGTGT TTTTGATTAT AGGACTAATA ATGTCGAGAA CATTCCTATT 
CTCTTAAACA ATTTTAAAAA ATTCATCAAG AAATATCAAT TTGATGATGT CTTTGAAAAT 
GATATCATAG AAATCGATCC TCGTGAAAAT GAAATCTTGT GCAAGATAAT CAAAGAAGGA 
CTCGGTGAAA GTTTAGATAT CATGAACACA AATACAACTG ACATTTTTAG GATAATCGAT 
GGTTTAAAAA ACAAATATAG AAGTTTACAT GGTAGAGATG TCAGAATTAG AGCCTGGGAA 
AAGGTTTTGG TTGATACAAC ATGTAGAAAT TCCGCATTGT TAATGAATAA ACTTCAAAAG 
TTGGTACTAA TGGAAAAATG GATTTTTTCT AAATGCTGCC AAGATTGTCC TAATCTAAAG 
GATTACCTAC AAGAAGCTAT CATGGGAACC TTACATGAAT CCTTAAGAAA TTCTGTGAAA 
CAACGTTTGT ACAACATTCC ACATAACGTA GGAATTAATC ACGAAGAATT TCTAATCAAT 
ACTGTTATTG AAACAGTAAT TGATTTGAGC CCAATTGCAG ACGATCAAAT AGAAAATAGC 
TGCATGTATT GCAAATCTGT TTTCCATTGC TCAATTAACT GCAAAAAGAA ACCAAATAGG 
GAACTTGGCC TGACTCGACC AATTTCTCAA AAACCTATTA TCTACAAGGT GCACAGAGAC 
AACAACAACT TAAGTCCAGT GCAAAACGAA CAAAAGTCTT GGAACAAGAC ACAAAAAAAG 
TCAAACAAAG TGTACAACAG CAAAAAACTG GTAATTATTG ATACCGGTTC CGGCGTAAAC 
ATTACCAATG ACAAAACCTT ACTGCATAAT TACGAAGACA GTAATCGCAG TACACGATTT 
TTTGGTATTG GGAAAAACAG TTCAGTGTCT GTTAAAGGGT ATGGCTATAT AAAAATCAAG 
AATGGTCACA ACAATACTGA CAATAAGTGT TTATTGACTT ACTATGTACC GGAAGAAGAA 
TCCACTATAA TCAGCTGTTA TGACTTAGCC AAGAAAACCA AAATGGTTTT AAGTCGAAAA 
TATACCAGAT TGGGAAACAA AATCATAAAA ATTAAAACCA AGATAGTTAA TGGTGTCATT 
CACGTAAAAA TGAACGAGTT AATTGAACGT CCTTCCGATG ATTCAAAAAT AAATGCAATA 
AAACCTACTT CTTCTCCTGG ATTTAAACTA AATAAAAGGT CTATTACCTT GGAAGATGCT 
CATAAAAGAA TGGGCCATAC AGGAATTCAA CAAATTGAAA ATTCCATAAA ACATAATCAT 
TATGAAGAAT CCCTTGACTT AATCAAAGAA CCAAATGAAT TTTGGTGTCA AACCTGTAAA 
ATCTCTAAAG CCACGAAAAG AAATCATTAT ACCGGGTCTA TGAATAATCA TAGTACTGAT 
CATGAACCAG GATCATCATG GTGCATGGAT ATATTTGGCC CTGTATCAAG TTCAAACGCG 
GACACTAAAA GGTACATGCT TATTATGGTG GATAACAACA CGAGATATTG CATGACCTCC 
ACACACTTCA ATAAGAATGC TGAAACTATT TTAGCTCAAA TCAGAAAGAA TATTCAGTAC 
GTGGAAACAC AATTTGACAG GAAAGTCAGA GAAATTAATT CAGACAGAGG TACTGAATTC 
ACAAATGATC AGATAGAAGA ATATTTTATT TCAAAAGGAA TACATCACAT ACTTACTTCT 
ACACAAGATC ATGCTGCTAA TGGAAGAGCA GAAAGATACA TCAGAACAAT AGTAACTGAT 
GCAACAACAC TCCTAAGACA AAGTAACTTA AGAGTAAAAT TTTGGGAATA CGCAGTAACT 
TCTGCTACCA ATATAAGAAA TTGCCTGGAA CACAAAAGTA CAGGTAAACT ACCATTGAAG 
GCAATCTCAC GTCAACCTGT TACAGTGAGA TTAATGTCAT TCTTACCATT TGGCGAAAAA 
GGAATAATTT GGAATCATAA TCACAAAAAA TTGAAACCAT CTGGACTTCC TTCTATAATT 
CTATGCAAAG ATCCAAATAG TTATGGATAC AAATTCTTTA TACCATCCAA AAATAAAATT 
GTCACATCTG ATAATTATAC AATTCCCAAC TATACGATGG ACGGTAGAGT AAGAAATACT 
CAGAATATTT ACAAGAGTCA TCAATTCAGT TCACATAATG ATAATGAAGA AGATCAAATC 
GAAACGGTCA CAAACTTATG TGAAGCTTTG GAAAACTACG AAGATGATAA TAAACCAATT 
ACTCGCCTGG AAGATTTGTT CACAGAGGAA GAGTTATCTC AAATAGACTC AAACGCAAAA 
TACCCATCTC CTAGTAATAA CCTAGAAGGG GACTTGGATT ACGTATTTTC TGATGTTGAG 
GAATCTGGAG ATTATGACGT TGAATCTGAA CTTTCAACGA CAAATACTTC AATCTCAACT 
GATAAAAACA AAATTTTGTC AAACAAGGAT TTTAATTCAG AACTTGCATC GACTGAAATA 
TCCATCAGTG AAATCGATAA GAAAGGATTA ATAAATACAA GTCATATTGA TGAAGATAAG 
TATGATGAAA AAGTCCACAG AATTCCATCG ATTATACAAG AGAAACTGGT AGGAAGTAAA 
AATACTATTA AAATCAATGA CGAAAACAGA ATCTCCGACA GAATTCGTAG TAAAAATATT 
GGGAGTATCT TAAACACTGG ACTCAGTAGA TGTGTAGATA TCACCGATGA ATCTATTACT 
AACAAAGATG AGTCAATGCA CAACGCAAAA CCCGAACTAA TTCAGGAGCA GTTCAATAAA 
ACAAATCATG AAACTTCGTT TCCTAAAGAA GGGAGCATTG GAACAAATGT AAAATTCCGA 
AATACAGACA ATGAGATTTC TTTAAAAACA GGCGATACGA GTTTACCAAT AAAAACTTTA 
GAAAGCATTA ACAATCACCA TAGTAATGAT TATTCCACAA ACAAAGTTGA AAAGTTTGAG 
AAGGAAAATC ATCATCCGCC CCCGATTGAG GACATTGTGG ATATGAGTGA TCAAACTGAT 
ATGGAATCAA ACTGTCAGGA TGGTAATAAC TTAAAAGAAT TAAAAGTCAC CGATAAAAAT 
GTACCAACTG ACAATGGAAC AAATGTGTCA CCAAGGTTGG AACAAAATAT TGAAGCATCT 
GGATCACCAG TACAAACAGT TAATAAAAGT GCCTTCTTAA ACAAAGAATT CAGTTCTTTG 
AACATGAAAA GAAAACGGAA AAGACACGAT AAAAACAATA GTCTAACAAG CTATGAATTA 
GAAAGAGATA AGAAGCGTTC AAAAAGGAAT CGAGTGAAAT TAATTCCAGA TAATATGGAA 
ACAGTTTCAG CACAAAAAAT TAGAGCCATA TATTATAATG AAGCTATTTC AAAAAATCCT 
GACCTCAAAG AAAAACATGA ATACAAACAG GCATATCATA AAGAATTACA GAATTTAAAA 
GATATGAAGG TATTTGATGT CGATGTGAAG TACAGTAGAT CAGAAATCCC TGATAATTTA 
ATAGTACCCA CCAACACGAT ATTCACAAAG AAAAGAAATG GGATTTATAA GGCTAGGATA 
GTCTGCAGAG GTGATACTCA GTCACCAGAC ACTTACAGTG TAATAACTAC AGAATCTTTA 
AATCACAATC ATATTAAGAT ATTCTTAATG ATTGCAAACA ACAGAAATAT GTTTATGAAG 
ACCCTGGATA TCAATCATGC ATTCCTATAT GCTAAATTGG AAGAAGAAAT ATACATCCCA 
CATCCGCATG ATAGGAGATG TGTAGTCAAG CTAAATAAGG CGTTATATGG TCTAAAACAG 
AGTCCTAAAG AATGGAATGA TCATCTAAGA CAATACTTGA ATGGAATTGG ACTGAAAGAT 
AACTCTTATA CTCCGGGATT ATACCAAACC GAGGATAAAA ATCTAATGAT TGCAGTCTAT 
GTTGATGACT GCGTAATTGC GGCAAGCAAT GAACAGAGAT TGGATGAATT CATAAACAAA 
TTGAAAAGTA ATTTTGAACT GAAAATTACA GGAACATTAA TAGATGATGT ACTCGATACA 
GATATATTAG GAATGGATCT AGTATACAAC AAAAGACTTG GTACTATCGA TTTAACATTA 
AAATCATTCA TAAATAGAAT GGATAAAAAA TACAACGAGG AATTGAAAAA GATTAGAAAA 
AGTTCAATTC CGCATATGTC AACTTATAAA ATAGATCCTA AGAAAGACGT ACTGCAAATG 
TCAGAAGAAG AGTTTAGACA AGGTGTTCTA AAGCTACAAC AATTACTAGG TGAACTAAAC 
TATGTCAGAC ACAAATGCAG ATACGACATT AATTTTGCTG TTAAGAAAGT GGCTAGACTA 
GTAAATTACC CACATGAAAG AGTCTTTTAT ATGATTTACA AAATAATCCA GTACTTGGTT 
CGGTATAAAG ATATTGGAAT ACACTATGAC CGAGACTGTA ATAAAGACAA GAAGGTTATT 
GCTATAACTG ATGCATCAGT TGGATCAGAA TATGATGCTC AATCAAGGAT TGGAGTTATA 
TTATGGTACG GTATGAATAT TTTTAATGTT TATTCTAACA AGAGCACAAA CAGATGTGTA 
TCATCAACAG AAGCAGAGCT TCATGCCATT TATGAAGGCT ATGCAGACTC AGAAACGTTG 
AAGGTAACAT TAAAGGAGCT AGGAGAAGGA GACAATAATG ACATTGTCAT GATCACTGAC 
TCAAAGCCAG CCATTCAAGG ATTAAATCGC AGCTATCAAC AACCAAAAGA GAAATTCACT 
TGGATAAAAA CTGAAATAAT AAAAGAAAAA ATTAAAGAGA AGAGTATAAA ACTGTTAAAA 
ATTACCGGCA AAGGTAATAT TGCTGATTTA CTAACAAAAC CAGTATCAGC ATCTGATTTT 
AAAAGATTTA TACAAGTATT AAAAAATAAA ATAACATCAC AGGATATTTT GGCCTCAACA 
GACTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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YHL009W-B cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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