DYSF gene cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey)

mRNA	Protein	Name
XM_010361942.1	XP_010360244.1	dysferlin isoform X9
XM_010361943.1	XP_010360245.1	dysferlin isoform X10
XM_010361941.2	XP_010360243.2	dysferlin isoform X9
XM_010361935.1	XP_010360237.1	dysferlin isoform X2
XM_010361944.1	XP_010360246.1	dysferlin isoform X11
XM_010361945.1	XP_010360247.1	dysferlin isoform X12
XM_010361946.1	XP_010360248.1	dysferlin isoform X13
XM_010361946.2	XP_010360248.2	dysferlin isoform X14
XM_010361947.2	XP_010360249.2	dysferlin isoform X15
XM_030921393.1	XP_030777253.1	dysferlin isoform X18
XM_030921394.1	XP_030777254.1	dysferlin isoform X19
XM_010361938.1	XP_010360240.1	dysferlin isoform X5
XM_010361935.2	XP_010360237.2	dysferlin isoform X3
XM_010361937.2	XP_010360239.2	dysferlin isoform X5
XM_010361942.2	XP_010360244.2	dysferlin isoform X10
XM_010361945.2	XP_010360247.2	dysferlin isoform X13
XM_030921395.1	XP_030777255.1	dysferlin isoform X20
XM_010361933.1	XP_010360235.1	dysferlin isoform X1
XM_010361936.1	XP_010360238.1	dysferlin isoform X3
XM_010361939.1	XP_010360241.1	dysferlin isoform X6
XM_010361941.1	XP_010360243.1	dysferlin isoform X8
XM_030921390.1	XP_030777250.1	dysferlin isoform X1
XM_010361933.2	XP_010360235.2	dysferlin isoform X2
XM_010361938.2	XP_010360240.2	dysferlin isoform X6
XM_030921392.1	XP_030777252.1	dysferlin isoform X17
XM_010361937.1	XP_010360239.1	dysferlin isoform X4
XM_010361940.1	XP_010360242.1	dysferlin isoform X7
XM_010361947.1	XP_010360249.1	dysferlin isoform X14
XM_010361948.1	XP_010360250.1	dysferlin isoform X15
XM_010361936.2	XP_010360238.2	dysferlin isoform X4
XM_010361939.2	XP_010360241.2	dysferlin isoform X7
XM_010361940.2	XP_010360242.2	dysferlin isoform X8
XM_010361943.2	XP_010360245.2	dysferlin isoform X11
XM_010361944.2	XP_010360246.2	dysferlin isoform X12
XM_010361948.2	XP_010360250.2	dysferlin isoform X16

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORh253205	XM_030921392.1 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORh253206	XM_030921393.1 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORh253207	XM_030921394.1 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORh253208	XM_030921395.1 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORh138127	XM_010361933.1 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORh138128	XM_010361935.1 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORh138129	XM_010361936.1 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORh138130	XM_010361937.1 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORh138131	XM_010361938.1 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORh138132	XM_010361939.1 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORh138133	XM_010361940.1 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORh138134	XM_010361941.1 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORh138135	XM_010361942.1 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORh138136	XM_010361943.1 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORh138137	XM_010361944.1 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORh138138	XM_010361945.1 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORh138139	XM_010361946.1 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORh138140	XM_010361947.1 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORh138141	XM_010361948.1 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORh253189	XM_030921390.1 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORh253190	XM_010361933.2 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORh253191	XM_010361935.2 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORh253192	XM_010361936.2 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORh253193	XM_010361937.2 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORh253194	XM_010361938.2 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORh253195	XM_010361939.2 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORh253196	XM_010361940.2 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORh253197	XM_010361941.2 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORh253198	XM_010361942.2 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORh253199	XM_010361943.2 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORh253200	XM_010361944.2 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORh253201	XM_010361945.2 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORh253202	XM_010361946.2 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORh253203	XM_010361947.2 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORh253204	XM_010361948.2 Latest version!	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

DYSF ( XM_030921392.1 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_030777252.1 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X18, mRNA.

Price & Availability↑

CloneID	ORh253205
Clone ID Related Accession (Same CDS sequence)	XM_030921392.1
Accession Version	XM_030921392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044565.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Rhinopithecus roxellana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGTGGA GGCCCAGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCT GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCG 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAAACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TCCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCACC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGAGGA GACTGCAGGT GCCGTCAAGT CTTCGAAAGC CGCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGTTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG ACTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTCGAGGT CAGCATTGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGTAATGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGC TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGACCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TTGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA GGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCAGA GAAGACCCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACTGATGT GAATGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATA CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGTTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCATCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC ACTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGCCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGCGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAACCGAGT 
TTGGAACGGA TGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGATGGGG 
GAGCTGCTGG CCTCTTTTGA GCTTATCCAG AGAGAGAAGC CAGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGT CTGAGGACAC AGACTTGCCC 
TACCCACCAC CCCAAAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GGTCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAGCA TCTCCTCCCC GAGCCTTGTG GTAGAGTGTG GGGGCCAAAC GGTGCAGTCC 
TGTGTCATTA GGAACCTCCG GAAGAATCCC AACTTTGACA TCTGTACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCATCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCG TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCTACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATG AGGAAGAGTT TATCGATTGG TGGAGCAAAT TCTTTGCCTC CATAGGGGAG 
AGGGAAAAGT GCGGCTCCTA CTTGGAGAAG GATTTTGACA CCCTGAAGGT CTATGACACA 
CAGCTGGAGA ATGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTG 
TACCGGGGCA AGACGCAGGA GGAGACAGAA GATCCATCTG TCATTGGCGA ATTTAAGGGC 
CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCAGCCATCC CCATGCCCCC AAGACAGTTC 
CACCAGCTGG CCACGCAGGG ACCGCAGGAG TGCTTGGTCC GTATCTACAT TGTCCGAGCA 
TTTGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCCT GCACGTTGGA GCCTGTGTTT 
GGAAAGATGT TTGAGCTGAC CTGCACTCTG CCTCTAGAGA AGGACCTGAA GGTCACCCTC 
TACGACTATG ACCTCCTCTC CAAGGATGAA AAGATTGGTG AGACGGTCAT CGACCTGGAG 
AACAGGCTGC TGTCCAAGTT TGCGGCTCGC TGTGGACTCC CACAGACCTA CTGTGTCTCT 
GGACCGAACC AGTGGCGGGA CCAGCTCCAC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCGCAGAG TCAAGGCGCC TGTGTACCGG ACAGACCGTG TGATGTTTCA GGATAAAGAA 
TACTCCATTG AAGAAATAGA GGCTGGCAGG ATCCCAAACC CACACCTGGG CCCCATGGAG 
GAGCGTCTGG CTCTGCATGT GCTTCGGCAG CAGGGCCTGG TCCCAGAGCA CGTGGAGTCA 
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATTGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTGTTTC CGAAGGCCCT GGGGCGGCCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTGTATTATC TGGAATACCA GAGATGTGAT CCTGGATGAC 
CTGAGCCTCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GATTGGCTTT 
GAAGAACACA AGCAAAAGAC AGACGTGCAT TATCGCTCCC TGGGAGGTGA AGGCAACTTC 
AACTGGAGGT TCGTTTTCCC CTTCGACTAC CTGCCGGCTG AGCAAGTCTG TACGGTTGCC 
AAGAAGAGTT GGGTCCAGGC CTCTGCTCTG ACCCGGCACT CAGGACTTGG CACCCGTCGT 
CTGCAGGATG GTCACACCAG GGGAGAGTTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030777252.1
CDS	106..6018
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEEETAGAVKSSKAADLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLHPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKSWVQASALTRHSGLGTRRLQDGHTRGEL

Target ORF information:

RefSeq Version	XM_030921392.1
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030921392.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGTGGA GGCCCAGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCT GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCG 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAAACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TCCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCACC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGAGGA GACTGCAGGT GCCGTCAAGT CTTCGAAAGC CGCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGTTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG ACTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTCGAGGT CAGCATTGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGTAATGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGC TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGACCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TTGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA GGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCAGA GAAGACCCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACTGATGT GAATGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATA CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGTTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCATCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC ACTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGCCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGCGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAACCGAGT 
TTGGAACGGA TGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGATGGGG 
GAGCTGCTGG CCTCTTTTGA GCTTATCCAG AGAGAGAAGC CAGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGT CTGAGGACAC AGACTTGCCC 
TACCCACCAC CCCAAAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GGTCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAGCA TCTCCTCCCC GAGCCTTGTG GTAGAGTGTG GGGGCCAAAC GGTGCAGTCC 
TGTGTCATTA GGAACCTCCG GAAGAATCCC AACTTTGACA TCTGTACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCATCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCG TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCTACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATG AGGAAGAGTT TATCGATTGG TGGAGCAAAT TCTTTGCCTC CATAGGGGAG 
AGGGAAAAGT GCGGCTCCTA CTTGGAGAAG GATTTTGACA CCCTGAAGGT CTATGACACA 
CAGCTGGAGA ATGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTG 
TACCGGGGCA AGACGCAGGA GGAGACAGAA GATCCATCTG TCATTGGCGA ATTTAAGGGC 
CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCAGCCATCC CCATGCCCCC AAGACAGTTC 
CACCAGCTGG CCACGCAGGG ACCGCAGGAG TGCTTGGTCC GTATCTACAT TGTCCGAGCA 
TTTGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCCT GCACGTTGGA GCCTGTGTTT 
GGAAAGATGT TTGAGCTGAC CTGCACTCTG CCTCTAGAGA AGGACCTGAA GGTCACCCTC 
TACGACTATG ACCTCCTCTC CAAGGATGAA AAGATTGGTG AGACGGTCAT CGACCTGGAG 
AACAGGCTGC TGTCCAAGTT TGCGGCTCGC TGTGGACTCC CACAGACCTA CTGTGTCTCT 
GGACCGAACC AGTGGCGGGA CCAGCTCCAC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCGCAGAG TCAAGGCGCC TGTGTACCGG ACAGACCGTG TGATGTTTCA GGATAAAGAA 
TACTCCATTG AAGAAATAGA GGCTGGCAGG ATCCCAAACC CACACCTGGG CCCCATGGAG 
GAGCGTCTGG CTCTGCATGT GCTTCGGCAG CAGGGCCTGG TCCCAGAGCA CGTGGAGTCA 
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATTGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTGTTTC CGAAGGCCCT GGGGCGGCCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTGTATTATC TGGAATACCA GAGATGTGAT CCTGGATGAC 
CTGAGCCTCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GATTGGCTTT 
GAAGAACACA AGCAAAAGAC AGACGTGCAT TATCGCTCCC TGGGAGGTGA AGGCAACTTC 
AACTGGAGGT TCGTTTTCCC CTTCGACTAC CTGCCGGCTG AGCAAGTCTG TACGGTTGCC 
AAGAAGAGTT GGGTCCAGGC CTCTGCTCTG ACCCGGCACT CAGGACTTGG CACCCGTCGT 
CTGCAGGATG GTCACACCAG GGGAGAGTTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_030921393.1 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_030777253.1 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X19, mRNA.

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CloneID	ORh253206
Clone ID Related Accession (Same CDS sequence)	XM_030921393.1
Accession Version	XM_030921393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044565.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Rhinopithecus roxellana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGTGGA GGCCCAGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCT GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCG 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAAACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TCCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCACC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGAGGA GACTGCAGGT GCCGTCAAGT CTTCGAAAGC CGCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGTTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG ACTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTCGAGGT CAGCATTGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGTAATGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGC TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGACCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TTGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA GGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCAGA GAAGACCCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACTGATGT GAATGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATA CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGTTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCATCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC ACTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGCCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGCGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAACCGAGT 
TTGGAACGGA TGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGATGGGG 
GAGCTGCTGG CCTCTTTTGA GCTTATCCAG AGAGAGAAGC CAGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGT CTGAGGACAC AGACTTGCCC 
TACCCACCAC CCCAAAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GGTCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAGCA TCTCCTCCCC GAGCCTTGTG GTAGAGTGTG GGGGCCAAAC GGTGCAGTCC 
TGTGTCATTA GGAACCTCCG GAAGAATCCC AACTTTGACA TCTGTACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCATCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCG TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCTACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATG AGGAAGAGTT TATCGATTGG TGGAGCAAAT TCTTTGCCTC CATAGGGGAG 
AGGGAAAAGT GCGGCTCCTA CTTGGAGAAG GATTTTGACA CCCTGAAGGT CTATGACACA 
CAGCTGGAGA ATGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTG 
TACCGGGGCA AGACGCAGGA GGAGACAGAA GATCCATCTG TCATTGGCGA ATTTAAGGGC 
CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCAGCCATCC CCATGCCCCC AAGACAGTTC 
CACCAGCTGG CCACGCAGGG ACCGCAGGAG TGCTTGGTCC GTATCTACAT TGTCCGAGCA 
TTTGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCCT GCACGTTGGA GCCTGTGTTT 
GGAAAGATGT TTGAGCTGAC CTGCACTCTG CCTCTAGAGA AGGACCTGAA GGTCACCCTC 
TACGACTATG ACCTCCTCTC CAAGGATGAA AAGATTGGTG AGACGGTCAT CGACCTGGAG 
AACAGGCTGC TGTCCAAGTT TGCGGCTCGC TGTGGACTCC CACAGACCTA CTGTGTCTCT 
GGACCGAACC AGTGGCGGGA CCAGCTCCAC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCGCAGAG TCAAGGCGCC TGTGTACCGG ACAGACCGTG TGATGTTTCA GGATAAAGAA 
TACTCCATTG AAGAAATAGA GGCTGGCAGG ATCCCAAACC CACACCTGGG CCCCATGGAG 
GAGCGTCTGG CTCTGCATGT GCTTCGGCAG CAGGGCCTGG TCCCAGAGCA CGTGGAGTCA 
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATTGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTGTTTC CGAAGGCCCT GGGGCGGCCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTGTATTATC TGGAATACCA GAGATGTGAT CCTGGATGAC 
CTGAGCCTCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GATTGGCTTT 
GAAGAACACA AGCAAAAGAC AGACGTGCAT TATCGCTCCC TGGGAGGTGA AGGCAACTTC 
AACTGGAGGT TCGTTTTCCC CTTCGACTAC CTGCCGGCTG AGCAAGTCTG TACGGTTGCC 
AAGAAGGCAG CCTCCAGGCT GGAAGGGAAC ACTGCCTCTC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030777253.1
CDS	106..5970
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEEETAGAVKSSKAADLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLHPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKAASRLEGNTASL

Target ORF information:

RefSeq Version	XM_030921393.1
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030921393.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGTGGA GGCCCAGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCT GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCG 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAAACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TCCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCACC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGAGGA GACTGCAGGT GCCGTCAAGT CTTCGAAAGC CGCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGTTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG ACTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTCGAGGT CAGCATTGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGTAATGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGC TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGACCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TTGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA GGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCAGA GAAGACCCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACTGATGT GAATGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATA CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGTTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCATCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC ACTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGCCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGCGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAACCGAGT 
TTGGAACGGA TGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGATGGGG 
GAGCTGCTGG CCTCTTTTGA GCTTATCCAG AGAGAGAAGC CAGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGT CTGAGGACAC AGACTTGCCC 
TACCCACCAC CCCAAAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GGTCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAGCA TCTCCTCCCC GAGCCTTGTG GTAGAGTGTG GGGGCCAAAC GGTGCAGTCC 
TGTGTCATTA GGAACCTCCG GAAGAATCCC AACTTTGACA TCTGTACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCATCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCG TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCTACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATG AGGAAGAGTT TATCGATTGG TGGAGCAAAT TCTTTGCCTC CATAGGGGAG 
AGGGAAAAGT GCGGCTCCTA CTTGGAGAAG GATTTTGACA CCCTGAAGGT CTATGACACA 
CAGCTGGAGA ATGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTG 
TACCGGGGCA AGACGCAGGA GGAGACAGAA GATCCATCTG TCATTGGCGA ATTTAAGGGC 
CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCAGCCATCC CCATGCCCCC AAGACAGTTC 
CACCAGCTGG CCACGCAGGG ACCGCAGGAG TGCTTGGTCC GTATCTACAT TGTCCGAGCA 
TTTGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCCT GCACGTTGGA GCCTGTGTTT 
GGAAAGATGT TTGAGCTGAC CTGCACTCTG CCTCTAGAGA AGGACCTGAA GGTCACCCTC 
TACGACTATG ACCTCCTCTC CAAGGATGAA AAGATTGGTG AGACGGTCAT CGACCTGGAG 
AACAGGCTGC TGTCCAAGTT TGCGGCTCGC TGTGGACTCC CACAGACCTA CTGTGTCTCT 
GGACCGAACC AGTGGCGGGA CCAGCTCCAC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCGCAGAG TCAAGGCGCC TGTGTACCGG ACAGACCGTG TGATGTTTCA GGATAAAGAA 
TACTCCATTG AAGAAATAGA GGCTGGCAGG ATCCCAAACC CACACCTGGG CCCCATGGAG 
GAGCGTCTGG CTCTGCATGT GCTTCGGCAG CAGGGCCTGG TCCCAGAGCA CGTGGAGTCA 
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATTGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTGTTTC CGAAGGCCCT GGGGCGGCCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTGTATTATC TGGAATACCA GAGATGTGAT CCTGGATGAC 
CTGAGCCTCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GATTGGCTTT 
GAAGAACACA AGCAAAAGAC AGACGTGCAT TATCGCTCCC TGGGAGGTGA AGGCAACTTC 
AACTGGAGGT TCGTTTTCCC CTTCGACTAC CTGCCGGCTG AGCAAGTCTG TACGGTTGCC 
AAGAAGGCAG CCTCCAGGCT GGAAGGGAAC ACTGCCTCTC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_030921394.1 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_030777254.1 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X20, mRNA.

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CloneID	ORh253207
Clone ID Related Accession (Same CDS sequence)	XM_030921394.1
Accession Version	XM_030921394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Rhinopithecus roxellana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGACA CGGATCCACA AGTGGACGAT GCCGTGATGG ACAACGTGAA ACAGATCTTT 
GGCTTTGAGA GTAACAAGAA GAACTTGGTG GACCCCTTTG TGGAGGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CTTGGAGAAA ACGGCCAACC CTCAGTGGAA CCAGAACATC 
ACACTGCCTG CCATGTTCCC CTCCATGTGT GAAAAAATGA GGATTCGTGT CATAGACTGG 
GATCGCCTCA CTCACAATGA CATCGTGGCT ACCACCGACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGAGGAGACT GCAGGTGCCG TCAAGTCTTC GAAAGCCGCA 
GACCTGGATG ACCACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTACAT CAACCTCTAT 
GGCAGTCCCA GAGAGTTCAC GGGTTTCCTG GACCCCTACG CAGAGCTCAA CACAGGCAAG 
GGGGAAGGTG TGGCTTATCG TGGCCGACTT CTGCTTTCCC TGGAGACCAA GCTGGTGGAG 
CACAGTGAGC AGAAGGTGGA GGACCTTCCT GCGGATGACA TCCTCCGGGT GGAGAAGTAC 
CTTAGGAGGC GCAAGTACTC CCTGTTTGCG GCCTTCTACT CGGCCACCAT GCTGCAGGAT 
GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ATGGGAACAA GTTCGACATG 
ACCTGCCTGC CGCTGGCCTC CACCACTCAG TATAGCCGTG CAGTCTTTGA CGGGTGCCAC 
TACTACTACC TACCCTGGGG TAATGTGAAA CCTGTGGTGG TGCTGTCATC CTACTGGGAG 
GACATCAGCC ATAGAATCGA GACTCAGAAC CAGCTGCTTG GGATTGCTGA CCGGCTGGAA 
GCTGGCCTGG AGCAGGTCCA CCTGGCCCTG AAGGCGCAGT GCTCCACGGA GGACGTGGAC 
TCACTGGTGG CTCAGCTGAT GGATGAGCTC ATCGCAGGCT GCAGCCAGCC TCTGGGTGAT 
ATCCATGAGA TACCCTCTGC CACCCACCTG GACGAGTACC TGTACCGGCT GCGCACCCGT 
CACCTGAGCC AAATCACGGA GGCTGCCCTG ACCCTGAAGC TTGGCCACAG TGAGCTCCCT 
GCTGCTCTGG ATCAGGCTGA GGACTGGCTC CTGCGTCTGC GTGCCCTTGC TGAGGAGCCC 
CAGAACAGCC TGCCGGATAT CATCATCTGG ATGCTGCAGG GAGACAAGCG TGTGGCATAC 
CAGCGGGTGC CCGCCCACCA AGTCCTCTTC TCCCGGCGGG GTGCCAACTA CTGTGGCAAG 
AATTGTGGGA AGTTGCAGAC AATCTTTCTG AAATATCCGA TGGAGAAGGT GCCTGGGGCC 
CGGATGCCAG TGCAGATACG GGTCAAGCTG TGGTTTGGGC TCTCTGTGGA TGAGAAGGAG 
TTCAACCAGT TTGCTGAGGG GAAGCTGTCT GTCTTTGCTG AAACCTATGA GAACCAGACC 
AAGTTGGCCC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAC 
GTCACCGGCA AGATCAAGCT ACCCAAGGAG AGCTTCCGCC CCTCGGCCGG CTGGACCTGG 
GCTGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTGCTCC ATGACATGGA CGCCGGTCAC 
CTGAGCTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TTCCCGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC TGATGTGAAT GGGGAGAAGG TGCTTCCCAA AGATGACATT 
GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAGGAATGGT CCACAGACCT CAACCGGGCT 
GTCGATGAGC AAGGCTGGGA GTATAGCATC ACCATACCCC CGGACCGGAA GCCGAAGCAC 
TGGGTCCCTG TTGAGAAGAT GTACTACACA CACCGACGGC GGCGCTGGGT GCGCCTGCGC 
AGGAGGGATC TCAGCCAGAT GGAAGCGCTG AAGAGGCATA GGCAGGCGGA GGCGGAGGGC 
GAGGGCTGGG AGTACGCCTC TCTTTTTGGC TGGAAGTTCC ATCTCGAGTA CCGCAAGACA 
GACGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CCCTGGAGAA GACGGGGCCT 
GCAGCTGTGT TTGCCCTCGA GGGGGCACTG GGCGGCGTGG TGGACGACAA GAGTGAAGAT 
TCCATGTCCG CCTCCACCTT GAGCTTTGGT GTGAACAGAC CCACAATTTC CTGCATATTT 
GACTATGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA CCTGGCTGCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAT GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACGGTGG TGGCGAAGAA CACCCTTAAC CCCACCTGGG ACCAGACGCT CATCTTCTAC 
GAGATCGAGA TCTTCGGCGA GCCGGCCACA GTTGCTGAGC AGCCGCCCAG CATTGTGGTG 
GAGCTGTACG ACCATGACAC TTACGGCGCA GACGAGTTTA TGGGCCGCTG CATCTGTCAA 
CCGAGTTTGG AACGGATGCC CCGACTGGCC TGGTTCCCAC TGACCAGGGG CAGCCAGCCG 
ATGGGGGAGC TGCTGGCCTC TTTTGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCACCAT 
ATTCCTGGTT TTGAGGTGCA GGAGACATCG AGGATCCTGG ATGAGTCTGA GGACACAGAC 
TTGCCCTACC CACCACCCCA AAGGGAGGCC AACATCTACA TGGTTCCTCA GAACATCAAG 
CCAGCGCTCC AGCGTACCGC CATTGAGGTC CTGGCATGGG GCCTGCGGAA CATGAAGAGT 
TACCAGCTGG CCAGCATCTC CTCCCCGAGC CTTGTGGTAG AGTGTGGGGG CCAAACGGTG 
CAGTCCTGTG TCATTAGGAA CCTCCGGAAG AATCCCAACT TTGACATCTG TACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC TACTGCCCCC CCATCACCAT CAAGGTCATC 
GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
AGCTTCCTGT GTGACCCCTA CTCGGCGGAG AGTCCGTCCC CACAGGGTGG CCCAGACGAT 
GTGAGCCTAC TCAGTCCTGG GGAAGACGTG CTCATCGACA TTGATGACAA GGAGCCCCTC 
ATCCCCATCC AGCTTGCAGA CGGTCTGTCG AGCTTGGCCC CTACTAACAC GGCTTCTCCT 
CCATCCAGTC CTCATGAGGA AGAGTTTATC GATTGGTGGA GCAAATTCTT TGCCTCCATA 
GGGGAGAGGG AAAAGTGCGG CTCCTACTTG GAGAAGGATT TTGACACCCT GAAGGTCTAT 
GACACACAGC TGGAGAATGT GGAGGCCTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC 
AAGCTGTACC GGGGCAAGAC GCAGGAGGAG ACAGAAGATC CATCTGTCAT TGGCGAATTT 
AAGGGCCTCT TCAAAATTTA TCCCCTCCCA GAAGACCCAG CCATCCCCAT GCCCCCAAGA 
CAGTTCCACC AGCTGGCCAC GCAGGGACCG CAGGAGTGCT TGGTCCGTAT CTACATTGTC 
CGAGCATTTG GCCTGCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TCCCCTGCAC GTTGGAGCCT 
GTGTTTGGAA AGATGTTTGA GCTGACCTGC ACTCTGCCTC TAGAGAAGGA CCTGAAGGTC 
ACCCTCTACG ACTATGACCT CCTCTCCAAG GATGAAAAGA TTGGTGAGAC GGTCATCGAC 
CTGGAGAACA GGCTGCTGTC CAAGTTTGCG GCTCGCTGTG GACTCCCACA GACCTACTGT 
GTCTCTGGAC CGAACCAGTG GCGGGACCAG CTCCACCCCT CCCAGCTCCT CCACCTCTTC 
TGCCAGCAGC GCAGAGTCAA GGCGCCTGTG TACCGGACAG ACCGTGTGAT GTTTCAGGAT 
AAAGAATACT CCATTGAAGA AATAGAGGCT GGCAGGATCC CAAACCCACA CCTGGGCCCC 
ATGGAGGAGC GTCTGGCTCT GCATGTGCTT CGGCAGCAGG GCCTGGTCCC AGAGCACGTG 
GAGTCACGGC CCCTCTACAG CCCCCTGCAG CCAGACATTG AGCAGGGGAA GCTGCAGATG 
TGGGTCGACC TGTTTCCGAA GGCCCTGGGG CGGCCTGGAC CTCCCTTCAA CATCACCCCA 
CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT ATTATCTGGA ATACCAGAGA TGTGATCCTG 
GATGACCTGA GCCTCACGGG GGAGAAGATG AGCGACATTT ATGTGAAAGG TTGGATGATT 
GGCTTTGAAG AACACAAGCA AAAGACAGAC GTGCATTATC GCTCCCTGGG AGGTGAAGGC 
AACTTCAACT GGAGGTTCGT TTTCCCCTTC GACTACCTGC CGGCTGAGCA AGTCTGTACG 
GTTGCCAAGA AGGATGCCTT CTGGAGGCTG GACAAGACTG AGAGCAAAAT CCCAGCACGA 
GTGATATTCC AGATCTGGGA CAATGACAAG TTCTCCTTTG ATGATTTTCT GGGCTCCCTG 
CAGCTCGATC TCAACCGCAT GCCCAAGCCA GCCAAGACAG CCGAGAAGTG CTCCTTGGAC 
CAGCTGGATG ACACTTACCA CCCAGAATGG TTTGTGTCCC TTTTTGAGCA GAAAACAGTG 
AAGGGCTGGT GGCCCTGTGT AGCAGAAGAG GGTGAGAAGA AGATACTGGC GGGCAAGCTG 
GAAATGACCT TGGAGATTGT AACAGAGAGT GAGCATGAGG AGCGGCCCGC TGGCCAGGGC 
CGGGATGAGC CCAACATGAA CCCTAAGCTT GAGGACCCAA GGCGCCCTGA CACCTCCTTC 
CTGTGGTTCA CCTCCCCATA CAAGACCATG AAGTTCATCC TGTGGCGGCG TTTCCGGTGG 
GCCATCATCC TCTTCATCAT CCTCTTCATC CTGCTGCTGT TCCTGGCCAT CTTCATCTAC 
GCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030777254.1
CDS	230..5377
Translation	MKDTDPQVDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEEETAGAVKSSKAADLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLHPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_030921394.1
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030921394.1

ORF Insert Sequence:

ATGAAGGACA CGGATCCACA AGTGGACGAT GCCGTGATGG ACAACGTGAA ACAGATCTTT 
GGCTTTGAGA GTAACAAGAA GAACTTGGTG GACCCCTTTG TGGAGGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CTTGGAGAAA ACGGCCAACC CTCAGTGGAA CCAGAACATC 
ACACTGCCTG CCATGTTCCC CTCCATGTGT GAAAAAATGA GGATTCGTGT CATAGACTGG 
GATCGCCTCA CTCACAATGA CATCGTGGCT ACCACCGACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGAGGAGACT GCAGGTGCCG TCAAGTCTTC GAAAGCCGCA 
GACCTGGATG ACCACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTACAT CAACCTCTAT 
GGCAGTCCCA GAGAGTTCAC GGGTTTCCTG GACCCCTACG CAGAGCTCAA CACAGGCAAG 
GGGGAAGGTG TGGCTTATCG TGGCCGACTT CTGCTTTCCC TGGAGACCAA GCTGGTGGAG 
CACAGTGAGC AGAAGGTGGA GGACCTTCCT GCGGATGACA TCCTCCGGGT GGAGAAGTAC 
CTTAGGAGGC GCAAGTACTC CCTGTTTGCG GCCTTCTACT CGGCCACCAT GCTGCAGGAT 
GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ATGGGAACAA GTTCGACATG 
ACCTGCCTGC CGCTGGCCTC CACCACTCAG TATAGCCGTG CAGTCTTTGA CGGGTGCCAC 
TACTACTACC TACCCTGGGG TAATGTGAAA CCTGTGGTGG TGCTGTCATC CTACTGGGAG 
GACATCAGCC ATAGAATCGA GACTCAGAAC CAGCTGCTTG GGATTGCTGA CCGGCTGGAA 
GCTGGCCTGG AGCAGGTCCA CCTGGCCCTG AAGGCGCAGT GCTCCACGGA GGACGTGGAC 
TCACTGGTGG CTCAGCTGAT GGATGAGCTC ATCGCAGGCT GCAGCCAGCC TCTGGGTGAT 
ATCCATGAGA TACCCTCTGC CACCCACCTG GACGAGTACC TGTACCGGCT GCGCACCCGT 
CACCTGAGCC AAATCACGGA GGCTGCCCTG ACCCTGAAGC TTGGCCACAG TGAGCTCCCT 
GCTGCTCTGG ATCAGGCTGA GGACTGGCTC CTGCGTCTGC GTGCCCTTGC TGAGGAGCCC 
CAGAACAGCC TGCCGGATAT CATCATCTGG ATGCTGCAGG GAGACAAGCG TGTGGCATAC 
CAGCGGGTGC CCGCCCACCA AGTCCTCTTC TCCCGGCGGG GTGCCAACTA CTGTGGCAAG 
AATTGTGGGA AGTTGCAGAC AATCTTTCTG AAATATCCGA TGGAGAAGGT GCCTGGGGCC 
CGGATGCCAG TGCAGATACG GGTCAAGCTG TGGTTTGGGC TCTCTGTGGA TGAGAAGGAG 
TTCAACCAGT TTGCTGAGGG GAAGCTGTCT GTCTTTGCTG AAACCTATGA GAACCAGACC 
AAGTTGGCCC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAC 
GTCACCGGCA AGATCAAGCT ACCCAAGGAG AGCTTCCGCC CCTCGGCCGG CTGGACCTGG 
GCTGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTGCTCC ATGACATGGA CGCCGGTCAC 
CTGAGCTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TTCCCGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC TGATGTGAAT GGGGAGAAGG TGCTTCCCAA AGATGACATT 
GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAGGAATGGT CCACAGACCT CAACCGGGCT 
GTCGATGAGC AAGGCTGGGA GTATAGCATC ACCATACCCC CGGACCGGAA GCCGAAGCAC 
TGGGTCCCTG TTGAGAAGAT GTACTACACA CACCGACGGC GGCGCTGGGT GCGCCTGCGC 
AGGAGGGATC TCAGCCAGAT GGAAGCGCTG AAGAGGCATA GGCAGGCGGA GGCGGAGGGC 
GAGGGCTGGG AGTACGCCTC TCTTTTTGGC TGGAAGTTCC ATCTCGAGTA CCGCAAGACA 
GACGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CCCTGGAGAA GACGGGGCCT 
GCAGCTGTGT TTGCCCTCGA GGGGGCACTG GGCGGCGTGG TGGACGACAA GAGTGAAGAT 
TCCATGTCCG CCTCCACCTT GAGCTTTGGT GTGAACAGAC CCACAATTTC CTGCATATTT 
GACTATGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA CCTGGCTGCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAT GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACGGTGG TGGCGAAGAA CACCCTTAAC CCCACCTGGG ACCAGACGCT CATCTTCTAC 
GAGATCGAGA TCTTCGGCGA GCCGGCCACA GTTGCTGAGC AGCCGCCCAG CATTGTGGTG 
GAGCTGTACG ACCATGACAC TTACGGCGCA GACGAGTTTA TGGGCCGCTG CATCTGTCAA 
CCGAGTTTGG AACGGATGCC CCGACTGGCC TGGTTCCCAC TGACCAGGGG CAGCCAGCCG 
ATGGGGGAGC TGCTGGCCTC TTTTGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCACCAT 
ATTCCTGGTT TTGAGGTGCA GGAGACATCG AGGATCCTGG ATGAGTCTGA GGACACAGAC 
TTGCCCTACC CACCACCCCA AAGGGAGGCC AACATCTACA TGGTTCCTCA GAACATCAAG 
CCAGCGCTCC AGCGTACCGC CATTGAGGTC CTGGCATGGG GCCTGCGGAA CATGAAGAGT 
TACCAGCTGG CCAGCATCTC CTCCCCGAGC CTTGTGGTAG AGTGTGGGGG CCAAACGGTG 
CAGTCCTGTG TCATTAGGAA CCTCCGGAAG AATCCCAACT TTGACATCTG TACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC TACTGCCCCC CCATCACCAT CAAGGTCATC 
GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
AGCTTCCTGT GTGACCCCTA CTCGGCGGAG AGTCCGTCCC CACAGGGTGG CCCAGACGAT 
GTGAGCCTAC TCAGTCCTGG GGAAGACGTG CTCATCGACA TTGATGACAA GGAGCCCCTC 
ATCCCCATCC AGCTTGCAGA CGGTCTGTCG AGCTTGGCCC CTACTAACAC GGCTTCTCCT 
CCATCCAGTC CTCATGAGGA AGAGTTTATC GATTGGTGGA GCAAATTCTT TGCCTCCATA 
GGGGAGAGGG AAAAGTGCGG CTCCTACTTG GAGAAGGATT TTGACACCCT GAAGGTCTAT 
GACACACAGC TGGAGAATGT GGAGGCCTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC 
AAGCTGTACC GGGGCAAGAC GCAGGAGGAG ACAGAAGATC CATCTGTCAT TGGCGAATTT 
AAGGGCCTCT TCAAAATTTA TCCCCTCCCA GAAGACCCAG CCATCCCCAT GCCCCCAAGA 
CAGTTCCACC AGCTGGCCAC GCAGGGACCG CAGGAGTGCT TGGTCCGTAT CTACATTGTC 
CGAGCATTTG GCCTGCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TCCCCTGCAC GTTGGAGCCT 
GTGTTTGGAA AGATGTTTGA GCTGACCTGC ACTCTGCCTC TAGAGAAGGA CCTGAAGGTC 
ACCCTCTACG ACTATGACCT CCTCTCCAAG GATGAAAAGA TTGGTGAGAC GGTCATCGAC 
CTGGAGAACA GGCTGCTGTC CAAGTTTGCG GCTCGCTGTG GACTCCCACA GACCTACTGT 
GTCTCTGGAC CGAACCAGTG GCGGGACCAG CTCCACCCCT CCCAGCTCCT CCACCTCTTC 
TGCCAGCAGC GCAGAGTCAA GGCGCCTGTG TACCGGACAG ACCGTGTGAT GTTTCAGGAT 
AAAGAATACT CCATTGAAGA AATAGAGGCT GGCAGGATCC CAAACCCACA CCTGGGCCCC 
ATGGAGGAGC GTCTGGCTCT GCATGTGCTT CGGCAGCAGG GCCTGGTCCC AGAGCACGTG 
GAGTCACGGC CCCTCTACAG CCCCCTGCAG CCAGACATTG AGCAGGGGAA GCTGCAGATG 
TGGGTCGACC TGTTTCCGAA GGCCCTGGGG CGGCCTGGAC CTCCCTTCAA CATCACCCCA 
CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT ATTATCTGGA ATACCAGAGA TGTGATCCTG 
GATGACCTGA GCCTCACGGG GGAGAAGATG AGCGACATTT ATGTGAAAGG TTGGATGATT 
GGCTTTGAAG AACACAAGCA AAAGACAGAC GTGCATTATC GCTCCCTGGG AGGTGAAGGC 
AACTTCAACT GGAGGTTCGT TTTCCCCTTC GACTACCTGC CGGCTGAGCA AGTCTGTACG 
GTTGCCAAGA AGGATGCCTT CTGGAGGCTG GACAAGACTG AGAGCAAAAT CCCAGCACGA 
GTGATATTCC AGATCTGGGA CAATGACAAG TTCTCCTTTG ATGATTTTCT GGGCTCCCTG 
CAGCTCGATC TCAACCGCAT GCCCAAGCCA GCCAAGACAG CCGAGAAGTG CTCCTTGGAC 
CAGCTGGATG ACACTTACCA CCCAGAATGG TTTGTGTCCC TTTTTGAGCA GAAAACAGTG 
AAGGGCTGGT GGCCCTGTGT AGCAGAAGAG GGTGAGAAGA AGATACTGGC GGGCAAGCTG 
GAAATGACCT TGGAGATTGT AACAGAGAGT GAGCATGAGG AGCGGCCCGC TGGCCAGGGC 
CGGGATGAGC CCAACATGAA CCCTAAGCTT GAGGACCCAA GGCGCCCTGA CACCTCCTTC 
CTGTGGTTCA CCTCCCCATA CAAGACCATG AAGTTCATCC TGTGGCGGCG TTTCCGGTGG 
GCCATCATCC TCTTCATCAT CCTCTTCATC CTGCTGCTGT TCCTGGCCAT CTTCATCTAC 
GCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_030921395.1 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_030777255.1 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X21, mRNA.

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CloneID	ORh253208
Clone ID Related Accession (Same CDS sequence)	XM_030921395.1
Accession Version	XM_030921395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Rhinopithecus roxellana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATGGA AGAAACAAAA GCTCTGCAGC AAGATCTTGG AGAAAACGGC CAACCCTCAG 
TGGAACCAGA ACATCACACT GCCTGCCATG TTCCCCTCCA TGTGTGAAAA AATGAGGATT 
CGTGTCATAG ACTGGGATCG CCTCACTCAC AATGACATCG TGGCTACCAC CGACCTGAGT 
ATGTCGAAAA TCTCTGCCCC TGGAGGAGAA ATAGAAGAGG AGACTGCAGG TGCCGTCAAG 
TCTTCGAAAG CCGCAGACCT GGATGACCAC CTGGGCTTCC TCCCCACTTT TGGGCCCTGC 
TACATCAACC TCTATGGCAG TCCCAGAGAG TTCACGGGTT TCCTGGACCC CTACGCAGAG 
CTCAACACAG GCAAGGGGGA AGGTGTGGCT TATCGTGGCC GACTTCTGCT TTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG TGAGCAGAAG GTGGAGGACC TTCCTGCGGA TGACATCCTC 
CGGGTGGAGA AGTACCTTAG GAGGCGCAAG TACTCCCTGT TTGCGGCCTT CTACTCGGCC 
ACCATGCTGC AGGATGTGGA TGACGCCATC CAGTTCGAGG TCAGCATTGG GAACTATGGG 
AACAAGTTCG ACATGACCTG CCTGCCGCTG GCCTCCACCA CTCAGTATAG CCGTGCAGTC 
TTTGACGGGT GCCACTACTA CTACCTACCC TGGGGTAATG TGAAACCTGT GGTGGTGCTG 
TCATCCTACT GGGAGGACAT CAGCCATAGA ATCGAGACTC AGAACCAGCT GCTTGGGATT 
GCTGACCGGC TGGAAGCTGG CCTGGAGCAG GTCCACCTGG CCCTGAAGGC GCAGTGCTCC 
ACGGAGGACG TGGACTCACT GGTGGCTCAG CTGATGGATG AGCTCATCGC AGGCTGCAGC 
CAGCCTCTGG GTGATATCCA TGAGATACCC TCTGCCACCC ACCTGGACGA GTACCTGTAC 
CGGCTGCGCA CCCGTCACCT GAGCCAAATC ACGGAGGCTG CCCTGACCCT GAAGCTTGGC 
CACAGTGAGC TCCCTGCTGC TCTGGATCAG GCTGAGGACT GGCTCCTGCG TCTGCGTGCC 
CTTGCTGAGG AGCCCCAGAA CAGCCTGCCG GATATCATCA TCTGGATGCT GCAGGGAGAC 
AAGCGTGTGG CATACCAGCG GGTGCCCGCC CACCAAGTCC TCTTCTCCCG GCGGGGTGCC 
AACTACTGTG GCAAGAATTG TGGGAAGTTG CAGACAATCT TTCTGAAATA TCCGATGGAG 
AAGGTGCCTG GGGCCCGGAT GCCAGTGCAG ATACGGGTCA AGCTGTGGTT TGGGCTCTCT 
GTGGATGAGA AGGAGTTCAA CCAGTTTGCT GAGGGGAAGC TGTCTGTCTT TGCTGAAACC 
TATGAGAACC AGACCAAGTT GGCCCTGGTC GGGAACTGGG GCACAACGGG CCTCACCTAC 
CCCAAGTTTT CTGACGTCAC CGGCAAGATC AAGCTACCCA AGGAGAGCTT CCGCCCCTCG 
GCCGGCTGGA CCTGGGCTGG AGATTGGTTC GTGTGTCCAG AGAAGACCCT GCTCCATGAC 
ATGGACGCCG GTCACCTGAG CTTTGTGGAG GAGGTGTTTG AGAACCAGAC CCGGCTTCCC 
GGAGGCCAGT GGATCTACAT GAGTGACAAC TACACTGATG TGAATGGGGA GAAGGTGCTT 
CCCAAAGATG ACATTGAGTG CCCACTGGGC TGGAAGTGGG AAGATGAGGA ATGGTCCACA 
GACCTCAACC GGGCTGTCGA TGAGCAAGGC TGGGAGTATA GCATCACCAT ACCCCCGGAC 
CGGAAGCCGA AGCACTGGGT CCCTGTTGAG AAGATGTACT ACACACACCG ACGGCGGCGC 
TGGGTGCGCC TGCGCAGGAG GGATCTCAGC CAGATGGAAG CGCTGAAGAG GCATAGGCAG 
GCGGAGGCGG AGGGCGAGGG CTGGGAGTAC GCCTCTCTTT TTGGCTGGAA GTTCCATCTC 
GAGTACCGCA AGACAGACGC CTTCCGCCGC CGCCGCTGGC GCCGCCGCAT GGAGCCCCTG 
GAGAAGACGG GGCCTGCAGC TGTGTTTGCC CTCGAGGGGG CACTGGGCGG CGTGGTGGAC 
GACAAGAGTG AAGATTCCAT GTCCGCCTCC ACCTTGAGCT TTGGTGTGAA CAGACCCACA 
ATTTCCTGCA TATTTGACTA TGGGAACCGC TACCATCTAC GCTGCTACAT GTACCAGGCC 
CGGGACCTGG CTGCGATGGA CAAGGACTCT TTTTCTGATC CCTATGCCAT CGTCTCCTTC 
CTGCACCAGA GCCAGAAGAC GGTGGTGGCG AAGAACACCC TTAACCCCAC CTGGGACCAG 
ACGCTCATCT TCTACGAGAT CGAGATCTTC GGCGAGCCGG CCACAGTTGC TGAGCAGCCG 
CCCAGCATTG TGGTGGAGCT GTACGACCAT GACACTTACG GCGCAGACGA GTTTATGGGC 
CGCTGCATCT GTCAACCGAG TTTGGAACGG ATGCCCCGAC TGGCCTGGTT CCCACTGACC 
AGGGGCAGCC AGCCGATGGG GGAGCTGCTG GCCTCTTTTG AGCTTATCCA GAGAGAGAAG 
CCAGCCATCC ACCATATTCC TGGTTTTGAG GTGCAGGAGA CATCGAGGAT CCTGGATGAG 
TCTGAGGACA CAGACTTGCC CTACCCACCA CCCCAAAGGG AGGCCAACAT CTACATGGTT 
CCTCAGAACA TCAAGCCAGC GCTCCAGCGT ACCGCCATTG AGGTCCTGGC ATGGGGCCTG 
CGGAACATGA AGAGTTACCA GCTGGCCAGC ATCTCCTCCC CGAGCCTTGT GGTAGAGTGT 
GGGGGCCAAA CGGTGCAGTC CTGTGTCATT AGGAACCTCC GGAAGAATCC CAACTTTGAC 
ATCTGTACCC TCTTCATGGA AGTGATGCTG CCCAGGGAGG AGCTCTACTG CCCCCCCATC 
ACCATCAAGG TCATCGATAA CCGCCAGTTT GGCCGCCGGC CTGTGGTGGG CCAGTGTACC 
ATCCGCTCCC TGGAGAGCTT CCTGTGTGAC CCCTACTCGG CGGAGAGTCC GTCCCCACAG 
GGTGGCCCAG ACGATGTGAG CCTACTCAGT CCTGGGGAAG ACGTGCTCAT CGACATTGAT 
GACAAGGAGC CCCTCATCCC CATCCAGCTT GCAGACGGTC TGTCGAGCTT GGCCCCTACT 
AACACGGCTT CTCCTCCATC CAGTCCTCAT GAGGAAGAGT TTATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACTTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTCATTGGCG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCACGCAGG GACCGCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGTTGG AGCCTGTGTT TGGAAAGATG TTTGAGCTGA CCTGCACTCT GCCTCTAGAG 
AAGGACCTGA AGGTCACCCT CTACGACTAT GACCTCCTCT CCAAGGATGA AAAGATTGGT 
GAGACGGTCA TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGCGGCTCG CTGTGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCA CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCGCAGA GTCAAGGCGC CTGTGTACCG GACAGACCGT 
GTGATGTTTC AGGATAAAGA ATACTCCATT GAAGAAATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCCATGGA GGAGCGTCTG GCTCTGCATG TGCTTCGGCA GCAGGGCCTG 
GTCCCAGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATTGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTGTTT CCGAAGGCCC TGGGGCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACC 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGCTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCGTTTTCC CCTTCGACTA CCTGCCGGCT 
GAGCAAGTCT GTACGGTTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGAT ATTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACT TACCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTAGCAG AAGAGGGTGA GAAGAAGATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAACAG AGAGTGAGCA TGAGGAGCGG 
CCCGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCTGACACCT CCTTCCTGTG GTTCACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030777255.1
CDS	87..5129
Translation	MVWKKQKLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEEETAGAVKSSKAADLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLHPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_030921395.1
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030921395.1

ORF Insert Sequence:

ATGGTATGGA AGAAACAAAA GCTCTGCAGC AAGATCTTGG AGAAAACGGC CAACCCTCAG 
TGGAACCAGA ACATCACACT GCCTGCCATG TTCCCCTCCA TGTGTGAAAA AATGAGGATT 
CGTGTCATAG ACTGGGATCG CCTCACTCAC AATGACATCG TGGCTACCAC CGACCTGAGT 
ATGTCGAAAA TCTCTGCCCC TGGAGGAGAA ATAGAAGAGG AGACTGCAGG TGCCGTCAAG 
TCTTCGAAAG CCGCAGACCT GGATGACCAC CTGGGCTTCC TCCCCACTTT TGGGCCCTGC 
TACATCAACC TCTATGGCAG TCCCAGAGAG TTCACGGGTT TCCTGGACCC CTACGCAGAG 
CTCAACACAG GCAAGGGGGA AGGTGTGGCT TATCGTGGCC GACTTCTGCT TTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG TGAGCAGAAG GTGGAGGACC TTCCTGCGGA TGACATCCTC 
CGGGTGGAGA AGTACCTTAG GAGGCGCAAG TACTCCCTGT TTGCGGCCTT CTACTCGGCC 
ACCATGCTGC AGGATGTGGA TGACGCCATC CAGTTCGAGG TCAGCATTGG GAACTATGGG 
AACAAGTTCG ACATGACCTG CCTGCCGCTG GCCTCCACCA CTCAGTATAG CCGTGCAGTC 
TTTGACGGGT GCCACTACTA CTACCTACCC TGGGGTAATG TGAAACCTGT GGTGGTGCTG 
TCATCCTACT GGGAGGACAT CAGCCATAGA ATCGAGACTC AGAACCAGCT GCTTGGGATT 
GCTGACCGGC TGGAAGCTGG CCTGGAGCAG GTCCACCTGG CCCTGAAGGC GCAGTGCTCC 
ACGGAGGACG TGGACTCACT GGTGGCTCAG CTGATGGATG AGCTCATCGC AGGCTGCAGC 
CAGCCTCTGG GTGATATCCA TGAGATACCC TCTGCCACCC ACCTGGACGA GTACCTGTAC 
CGGCTGCGCA CCCGTCACCT GAGCCAAATC ACGGAGGCTG CCCTGACCCT GAAGCTTGGC 
CACAGTGAGC TCCCTGCTGC TCTGGATCAG GCTGAGGACT GGCTCCTGCG TCTGCGTGCC 
CTTGCTGAGG AGCCCCAGAA CAGCCTGCCG GATATCATCA TCTGGATGCT GCAGGGAGAC 
AAGCGTGTGG CATACCAGCG GGTGCCCGCC CACCAAGTCC TCTTCTCCCG GCGGGGTGCC 
AACTACTGTG GCAAGAATTG TGGGAAGTTG CAGACAATCT TTCTGAAATA TCCGATGGAG 
AAGGTGCCTG GGGCCCGGAT GCCAGTGCAG ATACGGGTCA AGCTGTGGTT TGGGCTCTCT 
GTGGATGAGA AGGAGTTCAA CCAGTTTGCT GAGGGGAAGC TGTCTGTCTT TGCTGAAACC 
TATGAGAACC AGACCAAGTT GGCCCTGGTC GGGAACTGGG GCACAACGGG CCTCACCTAC 
CCCAAGTTTT CTGACGTCAC CGGCAAGATC AAGCTACCCA AGGAGAGCTT CCGCCCCTCG 
GCCGGCTGGA CCTGGGCTGG AGATTGGTTC GTGTGTCCAG AGAAGACCCT GCTCCATGAC 
ATGGACGCCG GTCACCTGAG CTTTGTGGAG GAGGTGTTTG AGAACCAGAC CCGGCTTCCC 
GGAGGCCAGT GGATCTACAT GAGTGACAAC TACACTGATG TGAATGGGGA GAAGGTGCTT 
CCCAAAGATG ACATTGAGTG CCCACTGGGC TGGAAGTGGG AAGATGAGGA ATGGTCCACA 
GACCTCAACC GGGCTGTCGA TGAGCAAGGC TGGGAGTATA GCATCACCAT ACCCCCGGAC 
CGGAAGCCGA AGCACTGGGT CCCTGTTGAG AAGATGTACT ACACACACCG ACGGCGGCGC 
TGGGTGCGCC TGCGCAGGAG GGATCTCAGC CAGATGGAAG CGCTGAAGAG GCATAGGCAG 
GCGGAGGCGG AGGGCGAGGG CTGGGAGTAC GCCTCTCTTT TTGGCTGGAA GTTCCATCTC 
GAGTACCGCA AGACAGACGC CTTCCGCCGC CGCCGCTGGC GCCGCCGCAT GGAGCCCCTG 
GAGAAGACGG GGCCTGCAGC TGTGTTTGCC CTCGAGGGGG CACTGGGCGG CGTGGTGGAC 
GACAAGAGTG AAGATTCCAT GTCCGCCTCC ACCTTGAGCT TTGGTGTGAA CAGACCCACA 
ATTTCCTGCA TATTTGACTA TGGGAACCGC TACCATCTAC GCTGCTACAT GTACCAGGCC 
CGGGACCTGG CTGCGATGGA CAAGGACTCT TTTTCTGATC CCTATGCCAT CGTCTCCTTC 
CTGCACCAGA GCCAGAAGAC GGTGGTGGCG AAGAACACCC TTAACCCCAC CTGGGACCAG 
ACGCTCATCT TCTACGAGAT CGAGATCTTC GGCGAGCCGG CCACAGTTGC TGAGCAGCCG 
CCCAGCATTG TGGTGGAGCT GTACGACCAT GACACTTACG GCGCAGACGA GTTTATGGGC 
CGCTGCATCT GTCAACCGAG TTTGGAACGG ATGCCCCGAC TGGCCTGGTT CCCACTGACC 
AGGGGCAGCC AGCCGATGGG GGAGCTGCTG GCCTCTTTTG AGCTTATCCA GAGAGAGAAG 
CCAGCCATCC ACCATATTCC TGGTTTTGAG GTGCAGGAGA CATCGAGGAT CCTGGATGAG 
TCTGAGGACA CAGACTTGCC CTACCCACCA CCCCAAAGGG AGGCCAACAT CTACATGGTT 
CCTCAGAACA TCAAGCCAGC GCTCCAGCGT ACCGCCATTG AGGTCCTGGC ATGGGGCCTG 
CGGAACATGA AGAGTTACCA GCTGGCCAGC ATCTCCTCCC CGAGCCTTGT GGTAGAGTGT 
GGGGGCCAAA CGGTGCAGTC CTGTGTCATT AGGAACCTCC GGAAGAATCC CAACTTTGAC 
ATCTGTACCC TCTTCATGGA AGTGATGCTG CCCAGGGAGG AGCTCTACTG CCCCCCCATC 
ACCATCAAGG TCATCGATAA CCGCCAGTTT GGCCGCCGGC CTGTGGTGGG CCAGTGTACC 
ATCCGCTCCC TGGAGAGCTT CCTGTGTGAC CCCTACTCGG CGGAGAGTCC GTCCCCACAG 
GGTGGCCCAG ACGATGTGAG CCTACTCAGT CCTGGGGAAG ACGTGCTCAT CGACATTGAT 
GACAAGGAGC CCCTCATCCC CATCCAGCTT GCAGACGGTC TGTCGAGCTT GGCCCCTACT 
AACACGGCTT CTCCTCCATC CAGTCCTCAT GAGGAAGAGT TTATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACTTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTCATTGGCG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCACGCAGG GACCGCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGTTGG AGCCTGTGTT TGGAAAGATG TTTGAGCTGA CCTGCACTCT GCCTCTAGAG 
AAGGACCTGA AGGTCACCCT CTACGACTAT GACCTCCTCT CCAAGGATGA AAAGATTGGT 
GAGACGGTCA TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGCGGCTCG CTGTGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCA CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCGCAGA GTCAAGGCGC CTGTGTACCG GACAGACCGT 
GTGATGTTTC AGGATAAAGA ATACTCCATT GAAGAAATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCCATGGA GGAGCGTCTG GCTCTGCATG TGCTTCGGCA GCAGGGCCTG 
GTCCCAGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATTGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTGTTT CCGAAGGCCC TGGGGCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACC 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGCTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCGTTTTCC CCTTCGACTA CCTGCCGGCT 
GAGCAAGTCT GTACGGTTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGAT ATTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACT TACCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTAGCAG AAGAGGGTGA GAAGAAGATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAACAG AGAGTGAGCA TGAGGAGCGG 
CCCGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCTGACACCT CCTTCCTGTG GTTCACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361933.1 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360235.1 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X1, mRNA.

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CloneID	ORh138127
Clone ID Related Accession (Same CDS sequence)	XM_010361933.1
Accession Version	XM_010361933.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-20
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010797358.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rhinopithecus roxellana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGTGGAGGC CCAGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCTGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCGGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA AACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTCC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCACCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGAGGAGAC TGCAGGTGCC GTCAAGTCTT CGAAAGCCGC AGACCTGGAT 
GACCACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGTTTCCT GGACCCCTAC GCAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGACT TCTGCTTTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAG 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCGGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TCGAGGTCAG CATTGGGAAC TATGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTATAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAATGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCACTGGTG 
GCTCAGCTGA TGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ATACCCTCTG CCACCCACCT GGACGAGTAC CTGTACCGGC TGCGCACCCG TCACCTGAGC 
CAAATCACGG AGGCTGCCCT GACCCTGAAG CTTGGCCACA GTGAGCTCCC TGCTGCTCTG 
GATCAGGCTG AGGACTGGCT CCTGCGTCTG CGTGCCCTTG CTGAGGAGCC CCAGAACAGC 
CTGCCGGATA TCATCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGTTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACCAGAC CAAGTTGGCC 
CTGGTCGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACCGGC 
AAGATCAAGC TACCCAAGGA GAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCAGAGAA GACCCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTT 
GTGGAGGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CTGATGTGAA TGGGGAGAAG GTGCTTCCCA AAGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATACCC CCGGACCGGA AGCCGAAGCA CTGGGTCCCT 
GTTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CATCTCGAGT ACCGCAAGAC AGACGCCTTC 
CGCCGCCGCC GCTGGCGCCG CCGCATGGAG CCCCTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTCG AGGGGGCACT GGGCGGCGTG GTGGACGACA AGAGTGAAGA TTCCATGTCC 
GCCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACAATTT CCTGCATATT TGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGCGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG CAGCCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTACGGCGC AGACGAGTTT ATGGGCCGCT GCATCTGTCA ACCGAGTTTG 
GAACGGATGC CCCGACTGGC CTGGTTCCCA CTGACCAGGG GCAGCCAGCC GATGGGGGAG 
CTGCTGGCCT CTTTTGAGCT TATCCAGAGA GAGAAGCCAG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC GAGGATCCTG GATGAGTCTG AGGACACAGA CTTGCCCTAC 
CCACCACCCC AAAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATTGAGGT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAGCATCT CCTCCCCGAG CCTTGTGGTA GAGTGTGGGG GCCAAACGGT GCAGTCCTGT 
GTCATTAGGA ACCTCCGGAA GAATCCCAAC TTTGACATCT GTACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCA TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCGTCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC CCTACTAACA CGGCTTCTCC TCCATCCAGT 
CCTCATGAGG AAGAGTTTAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTCA TTGGCGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCA CGCAGGGACC GCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGTTGGAGCC TGTGTTTGGA 
AAGATGTTTG AGCTGACCTG CACTCTGCCT CTAGAGAAGG ACCTGAAGGT CACCCTCTAC 
GACTATGACC TCCTCTCCAA GGATGAAAAG ATTGGTGAGA CGGTCATCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CGCAGAGTCA AGGCGCCTGT GTACCGGACA GACCGTGTGA TGTTTCAGGA TAAAGAATAC 
TCCATTGAAG AAATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC CATGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCGGCAGCAG GGCCTGGTCC CAGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATT GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTGTTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGCTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCG TTTTCCCCTT CGACTACCTG CCGGCTGAGC AAGTCTGTAC GGTTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGATATTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCAAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GACACTTACC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAGATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAACAGAGAG TGAGCATGAG GAGCGGCCCG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCTG ACACCTCCTT CCTGTGGTTC 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360235.1
CDS	792..7190
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEEETAGAVKSSKAADLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLNLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361933.1
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361933.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGTGGAGGC CCAGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCTGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCGGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA AACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTCC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCACCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGAGGAGAC TGCAGGTGCC GTCAAGTCTT CGAAAGCCGC AGACCTGGAT 
GACCACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGTTTCCT GGACCCCTAC GCAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGACT TCTGCTTTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAG 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCGGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TCGAGGTCAG CATTGGGAAC TATGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTATAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAATGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCACTGGTG 
GCTCAGCTGA TGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ATACCCTCTG CCACCCACCT GGACGAGTAC CTGTACCGGC TGCGCACCCG TCACCTGAGC 
CAAATCACGG AGGCTGCCCT GACCCTGAAG CTTGGCCACA GTGAGCTCCC TGCTGCTCTG 
GATCAGGCTG AGGACTGGCT CCTGCGTCTG CGTGCCCTTG CTGAGGAGCC CCAGAACAGC 
CTGCCGGATA TCATCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGTTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACCAGAC CAAGTTGGCC 
CTGGTCGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACCGGC 
AAGATCAAGC TACCCAAGGA GAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCAGAGAA GACCCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTT 
GTGGAGGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CTGATGTGAA TGGGGAGAAG GTGCTTCCCA AAGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATACCC CCGGACCGGA AGCCGAAGCA CTGGGTCCCT 
GTTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CATCTCGAGT ACCGCAAGAC AGACGCCTTC 
CGCCGCCGCC GCTGGCGCCG CCGCATGGAG CCCCTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTCG AGGGGGCACT GGGCGGCGTG GTGGACGACA AGAGTGAAGA TTCCATGTCC 
GCCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACAATTT CCTGCATATT TGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGCGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG CAGCCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTACGGCGC AGACGAGTTT ATGGGCCGCT GCATCTGTCA ACCGAGTTTG 
GAACGGATGC CCCGACTGGC CTGGTTCCCA CTGACCAGGG GCAGCCAGCC GATGGGGGAG 
CTGCTGGCCT CTTTTGAGCT TATCCAGAGA GAGAAGCCAG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC GAGGATCCTG GATGAGTCTG AGGACACAGA CTTGCCCTAC 
CCACCACCCC AAAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATTGAGGT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAGCATCT CCTCCCCGAG CCTTGTGGTA GAGTGTGGGG GCCAAACGGT GCAGTCCTGT 
GTCATTAGGA ACCTCCGGAA GAATCCCAAC TTTGACATCT GTACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCA TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCGTCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC CCTACTAACA CGGCTTCTCC TCCATCCAGT 
CCTCATGAGG AAGAGTTTAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTCA TTGGCGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCA CGCAGGGACC GCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGTTGGAGCC TGTGTTTGGA 
AAGATGTTTG AGCTGACCTG CACTCTGCCT CTAGAGAAGG ACCTGAAGGT CACCCTCTAC 
GACTATGACC TCCTCTCCAA GGATGAAAAG ATTGGTGAGA CGGTCATCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CGCAGAGTCA AGGCGCCTGT GTACCGGACA GACCGTGTGA TGTTTCAGGA TAAAGAATAC 
TCCATTGAAG AAATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC CATGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCGGCAGCAG GGCCTGGTCC CAGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATT GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTGTTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGCTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCG TTTTCCCCTT CGACTACCTG CCGGCTGAGC AAGTCTGTAC GGTTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGATATTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCAAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GACACTTACC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAGATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAACAGAGAG TGAGCATGAG GAGCGGCCCG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCTG ACACCTCCTT CCTGTGGTTC 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361935.1 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360237.1 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X2, mRNA.

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CloneID	ORh138128
Clone ID Related Accession (Same CDS sequence)	XM_010361935.1
Accession Version	XM_010361935.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-20
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010797358.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rhinopithecus roxellana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGTGGA GGCCCAGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCT GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCG 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAAACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TCCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCACC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGTTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGAC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTCGAGGTCA GCATTGGGAA CTATGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGTAATGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGCTG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGACCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTT 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACCGG CAAGATCAAG CTACCCAAGG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCAGAGA AGACCCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACTGATGTGA ATGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATACC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGTTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCATCTCGAG 
TACCGCAAGA CAGACGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCAC TGGGCGGCGT GGTGGACGAC 
AAGAGTGAAG ATTCCATGTC CGCCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGCGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTCGGC GAGCCGGCCA CAGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTACGGCG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AACCGAGTTT GGAACGGATG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGATGGGGGA GCTGCTGGCC TCTTTTGAGC TTATCCAGAG AGAGAAGCCA 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGTCT 
GAGGACACAG ACTTGCCCTA CCCACCACCC CAAAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATTGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGGT AGAGTGTGGG 
GGCCAAACGG TGCAGTCCTG TGTCATTAGG AACCTCCGGA AGAATCCCAA CTTTGACATC 
TGTACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCGTC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCTACTAAC 
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTTA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACT TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTC 
ATTGGCGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC ACGCAGGGAC CGCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGTTGGAGC CTGTGTTTGG AAAGATGTTT GAGCTGACCT GCACTCTGCC TCTAGAGAAG 
GACCTGAAGG TCACCCTCTA CGACTATGAC CTCCTCTCCA AGGATGAAAA GATTGGTGAG 
ACGGTCATCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG CGGCTCGCTG TGGACTCCCA 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCGCAGAGTC AAGGCGCCTG TGTACCGGAC AGACCGTGTG 
ATGTTTCAGG ATAAAGAATA CTCCATTGAA GAAATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CCATGGAGGA GCGTCTGGCT CTGCATGTGC TTCGGCAGCA GGGCCTGGTC 
CCAGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT TGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTGTTTCCG AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAATACCAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGCTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC GTTTTCCCCT TCGACTACCT GCCGGCTGAG 
CAAGTCTGTA CGGTTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGATATT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCAA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGACACTTAC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT GTAGCAGAAG AGGGTGAGAA GAAGATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAACAGAGA GTGAGCATGA GGAGCGGCCC 
GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCT 
GACACCTCCT TCCTGTGGTT CACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360237.1
CDS	136..6495
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLNLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361935.1
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361935.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGTGGA GGCCCAGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCT GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCG 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAAACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TCCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCACC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGTTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGAC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTCGAGGTCA GCATTGGGAA CTATGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGTAATGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGCTG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGACCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTT 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACCGG CAAGATCAAG CTACCCAAGG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCAGAGA AGACCCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACTGATGTGA ATGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATACC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGTTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCATCTCGAG 
TACCGCAAGA CAGACGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCAC TGGGCGGCGT GGTGGACGAC 
AAGAGTGAAG ATTCCATGTC CGCCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGCGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTCGGC GAGCCGGCCA CAGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTACGGCG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AACCGAGTTT GGAACGGATG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGATGGGGGA GCTGCTGGCC TCTTTTGAGC TTATCCAGAG AGAGAAGCCA 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGTCT 
GAGGACACAG ACTTGCCCTA CCCACCACCC CAAAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATTGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGGT AGAGTGTGGG 
GGCCAAACGG TGCAGTCCTG TGTCATTAGG AACCTCCGGA AGAATCCCAA CTTTGACATC 
TGTACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCGTC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCTACTAAC 
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTTA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACT TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTC 
ATTGGCGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC ACGCAGGGAC CGCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGTTGGAGC CTGTGTTTGG AAAGATGTTT GAGCTGACCT GCACTCTGCC TCTAGAGAAG 
GACCTGAAGG TCACCCTCTA CGACTATGAC CTCCTCTCCA AGGATGAAAA GATTGGTGAG 
ACGGTCATCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG CGGCTCGCTG TGGACTCCCA 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCGCAGAGTC AAGGCGCCTG TGTACCGGAC AGACCGTGTG 
ATGTTTCAGG ATAAAGAATA CTCCATTGAA GAAATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CCATGGAGGA GCGTCTGGCT CTGCATGTGC TTCGGCAGCA GGGCCTGGTC 
CCAGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT TGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTGTTTCCG AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAATACCAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGCTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC GTTTTCCCCT TCGACTACCT GCCGGCTGAG 
CAAGTCTGTA CGGTTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGATATT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCAA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGACACTTAC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT GTAGCAGAAG AGGGTGAGAA GAAGATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAACAGAGA GTGAGCATGA GGAGCGGCCC 
GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCT 
GACACCTCCT TCCTGTGGTT CACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361936.1 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360238.1 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X3, mRNA.

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CloneID	ORh138129
Clone ID Related Accession (Same CDS sequence)	XM_010361936.1
Accession Version	XM_010361936.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-20
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010797358.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rhinopithecus roxellana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGTGGAGGC CCAGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCTGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCGGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA AACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTCC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCACCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGTTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGACTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTC GAGGTCAGCA TTGGGAACTA TGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AATGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGCTGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGA CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTTGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACCGGCAA GATCAAGCTA CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCAGAGAAGA CCCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACT GATGTGAATG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATACCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGT TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA TCTCGAGTAC 
CGCAAGACAG ACGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCACTGG GCGGCGTGGT GGACGACAAG 
AGTGAAGATT CCATGTCCGC CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGCGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTCGGCGAG CCGGCCACAG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TACGGCGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAAC CGAGTTTGGA ACGGATGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA TGGGGGAGCT GCTGGCCTCT TTTGAGCTTA TCCAGAGAGA GAAGCCAGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACT TGCCCTACCC ACCACCCCAA AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATTGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC 
CAAACGGTGC AGTCCTGTGT CATTAGGAAC CTCCGGAAGA ATCCCAACTT TGACATCTGT 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCGTCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC TACTAACACG 
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCACG CAGGGACCGC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT AGAGAAGGAC 
CTGAAGGTCA CCCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCA TGGAGGAGCG TCTGGCTCTG CATGTGCTTC GGCAGCAGGG CCTGGTCCCA 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCGTT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACGG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCAATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTACCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCTGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360238.1
CDS	792..7148
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLNLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361936.1
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361936.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGTGGAGGC CCAGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCTGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCGGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA AACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTCC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCACCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGTTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGACTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTC GAGGTCAGCA TTGGGAACTA TGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AATGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGCTGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGA CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTTGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACCGGCAA GATCAAGCTA CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCAGAGAAGA CCCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACT GATGTGAATG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATACCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGT TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA TCTCGAGTAC 
CGCAAGACAG ACGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCACTGG GCGGCGTGGT GGACGACAAG 
AGTGAAGATT CCATGTCCGC CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGCGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTCGGCGAG CCGGCCACAG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TACGGCGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAAC CGAGTTTGGA ACGGATGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA TGGGGGAGCT GCTGGCCTCT TTTGAGCTTA TCCAGAGAGA GAAGCCAGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACT TGCCCTACCC ACCACCCCAA AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATTGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC 
CAAACGGTGC AGTCCTGTGT CATTAGGAAC CTCCGGAAGA ATCCCAACTT TGACATCTGT 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCGTCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC TACTAACACG 
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCACG CAGGGACCGC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT AGAGAAGGAC 
CTGAAGGTCA CCCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCA TGGAGGAGCG TCTGGCTCTG CATGTGCTTC GGCAGCAGGG CCTGGTCCCA 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCGTT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACGG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCAATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTACCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCTGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361937.1 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360239.1 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X4, mRNA.

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CloneID	ORh138130
Clone ID Related Accession (Same CDS sequence)	XM_010361937.1
Accession Version	XM_010361937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-20
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010797358.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rhinopithecus roxellana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGTGGA GGCCCAGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCT GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCG 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAAACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TCCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCACC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGAGGA GACTGCAGGT GCCGTCAAGT CTTCGAAAGC CGCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGTTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG ACTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTCGAGGT CAGCATTGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGTAATGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGC TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGACCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TTGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA GGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCAGA GAAGACCCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACTGATGT GAATGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATA CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGTTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCATCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC ACTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGCCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGCGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAACCGAGT 
TTGGAACGGA TGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGATGGGG 
GAGCTGCTGG CCTCTTTTGA GCTTATCCAG AGAGAGAAGC CAGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGT CTGAGGACAC AGACTTGCCC 
TACCCACCAC CCCAAAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GGTCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAGCA TCTCCTCCCC GAGCCTTGTG GTAGAGTGTG GGGGCCAAAC GGTGCAGTCC 
TGTGTCATTA GGAACCTCCG GAAGAATCCC AACTTTGACA TCTGTACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCATCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCG TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGGAGG AAGAGTTTAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTCA TTGGCGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCA CGCAGGGACC GCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGTTGGAGCC TGTGTTTGGA 
AAGATGTTTG AGCTGACCTG CACTCTGCCT CTAGAGAAGG ACCTGAAGGT CACCCTCTAC 
GACTATGACC TCCTCTCCAA GGATGAAAAG ATTGGTGAGA CGGTCATCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CGCAGAGTCA AGGCGCCTGT GTACCGGACA GACCGTGTGA TGTTTCAGGA TAAAGAATAC 
TCCATTGAAG AAATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC CATGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCGGCAGCAG GGCCTGGTCC CAGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATT GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTGTTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGCTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCG TTTTCCCCTT CGACTACCTG CCGGCTGAGC AAGTCTGTAC GGTTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGATATTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCAAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GACACTTACC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAGATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAACAGAGAG TGAGCATGAG GAGCGGCCCG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCTG ACACCTCCTT CCTGTGGTTC 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360239.1
CDS	136..6474
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEEETAGAVKSSKAADLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLNLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361937.1
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361937.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGTGGA GGCCCAGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCT GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCG 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAAACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TCCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCACC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGAGGA GACTGCAGGT GCCGTCAAGT CTTCGAAAGC CGCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGTTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG ACTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTCGAGGT CAGCATTGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGTAATGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGC TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGACCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TTGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA GGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCAGA GAAGACCCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACTGATGT GAATGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATA CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGTTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCATCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC ACTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGCCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGCGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAACCGAGT 
TTGGAACGGA TGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGATGGGG 
GAGCTGCTGG CCTCTTTTGA GCTTATCCAG AGAGAGAAGC CAGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGT CTGAGGACAC AGACTTGCCC 
TACCCACCAC CCCAAAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GGTCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAGCA TCTCCTCCCC GAGCCTTGTG GTAGAGTGTG GGGGCCAAAC GGTGCAGTCC 
TGTGTCATTA GGAACCTCCG GAAGAATCCC AACTTTGACA TCTGTACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCATCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCG TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGGAGG AAGAGTTTAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTCA TTGGCGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCA CGCAGGGACC GCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGTTGGAGCC TGTGTTTGGA 
AAGATGTTTG AGCTGACCTG CACTCTGCCT CTAGAGAAGG ACCTGAAGGT CACCCTCTAC 
GACTATGACC TCCTCTCCAA GGATGAAAAG ATTGGTGAGA CGGTCATCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CGCAGAGTCA AGGCGCCTGT GTACCGGACA GACCGTGTGA TGTTTCAGGA TAAAGAATAC 
TCCATTGAAG AAATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC CATGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCGGCAGCAG GGCCTGGTCC CAGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATT GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTGTTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGCTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCG TTTTCCCCTT CGACTACCTG CCGGCTGAGC AAGTCTGTAC GGTTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGATATTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCAAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GACACTTACC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAGATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAACAGAGAG TGAGCATGAG GAGCGGCCCG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCTG ACACCTCCTT CCTGTGGTTC 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361938.1 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360240.1 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X5, mRNA.

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CloneID	ORh138131
Clone ID Related Accession (Same CDS sequence)	XM_010361938.1
Accession Version	XM_010361938.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-20
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010797358.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rhinopithecus roxellana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGTGGAGGC CCAGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCTGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCGGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA AACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTCC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCACCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGAGGAGAC TGCAGGTGCC GTCAAGTCTT CGAAAGCCGC AGACCTGGAT 
GACCACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGTTTCCT GGACCCCTAC GCAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGACT TCTGCTTTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAG 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCGGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TCGAGGTCAG CATTGGGAAC TATGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTATAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAATGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCACTGGTG 
GCTCAGCTGA TGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ATACCCTCTG CCACCCACCT GGACGAGTAC CTGTACCGGC TGCGCACCCG TCACCTGAGC 
CAAATCACGG AGGCTGCCCT GACCCTGAAG CTTGGCCACA GTGAGCTCCC TGCTGCTCTG 
GATCAGGCTG AGGACTGGCT CCTGCGTCTG CGTGCCCTTG CTGAGGAGCC CCAGAACAGC 
CTGCCGGATA TCATCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGTTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACCAGAC CAAGTTGGCC 
CTGGTCGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACCGGC 
AAGATCAAGC TACCCAAGGA GAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCAGAGAA GACCCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTT 
GTGGAGGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CTGATGTGAA TGGGGAGAAG GTGCTTCCCA AAGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATACCC CCGGACCGGA AGCCGAAGCA CTGGGTCCCT 
GTTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CATCTCGAGT ACCGCAAGAC AGACGCCTTC 
CGCCGCCGCC GCTGGCGCCG CCGCATGGAG CCCCTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTCG AGGGGGCACT GGGCGGCGTG GTGGACGACA AGAGTGAAGA TTCCATGTCC 
GCCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACAATTT CCTGCATATT TGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGCGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG CAGCCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTACGGCGC AGACGAGTTT ATGGGCCGCT GCATCTGTCA ACCGAGTTTG 
GAACGGATGC CCCGACTGGC CTGGTTCCCA CTGACCAGGG GCAGCCAGCC GATGGGGGAG 
CTGCTGGCCT CTTTTGAGCT TATCCAGAGA GAGAAGCCAG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC GAGGATCCTG GATGAGTCTG AGGACACAGA CTTGCCCTAC 
CCACCACCCC AAAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATTGAGGT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAGCATCT CCTCCCCGAG CCTTGTGGTA GAGTGTGGGG GCCAAACGGT GCAGTCCTGT 
GTCATTAGGA ACCTCCGGAA GAATCCCAAC TTTGACATCT GTACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCA TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCGTCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGGAGGAAG AGTTTATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACTTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTCATTG GCGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCCACGC AGGGACCGCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGT TGGAGCCTGT GTTTGGAAAG 
ATGTTTGAGC TGACCTGCAC TCTGCCTCTA GAGAAGGACC TGAAGGTCAC CCTCTACGAC 
TATGACCTCC TCTCCAAGGA TGAAAAGATT GGTGAGACGG TCATCGACCT GGAGAACAGG 
CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA CTCCCACAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC 
AGAGTCAAGG CGCCTGTGTA CCGGACAGAC CGTGTGATGT TTCAGGATAA AGAATACTCC 
ATTGAAGAAA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCCAT GGAGGAGCGT 
CTGGCTCTGC ATGTGCTTCG GCAGCAGGGC CTGGTCCCAG AGCACGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATTGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTG 
TTTCCGAAGG CCCTGGGGCG GCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT TATCTGGAAT ACCAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGC TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCGTTT TCCCCTTCGA CTACCTGCCG GCTGAGCAAG TCTGTACGGT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GATATTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCAATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC GAGAAGTGCT CCTTGGACCA GCTGGATGAC 
ACTTACCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCCTGTGTAG CAGAAGAGGG TGAGAAGAAG ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAA CAGAGAGTGA GCATGAGGAG CGGCCCGCTG GCCAGGGCCG GGATGAGCCC 
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCTGACA CCTCCTTCCT GTGGTTCACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTCATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360240.1
CDS	792..7127
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEEETAGAVKSSKAADLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLNLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361938.1
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361938.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGTGGAGGC CCAGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCTGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCGGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA AACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTCC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCACCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGAGGAGAC TGCAGGTGCC GTCAAGTCTT CGAAAGCCGC AGACCTGGAT 
GACCACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGTTTCCT GGACCCCTAC GCAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGACT TCTGCTTTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAG 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCGGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TCGAGGTCAG CATTGGGAAC TATGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTATAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAATGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCACTGGTG 
GCTCAGCTGA TGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ATACCCTCTG CCACCCACCT GGACGAGTAC CTGTACCGGC TGCGCACCCG TCACCTGAGC 
CAAATCACGG AGGCTGCCCT GACCCTGAAG CTTGGCCACA GTGAGCTCCC TGCTGCTCTG 
GATCAGGCTG AGGACTGGCT CCTGCGTCTG CGTGCCCTTG CTGAGGAGCC CCAGAACAGC 
CTGCCGGATA TCATCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGTTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACCAGAC CAAGTTGGCC 
CTGGTCGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACCGGC 
AAGATCAAGC TACCCAAGGA GAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCAGAGAA GACCCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTT 
GTGGAGGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CTGATGTGAA TGGGGAGAAG GTGCTTCCCA AAGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATACCC CCGGACCGGA AGCCGAAGCA CTGGGTCCCT 
GTTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CATCTCGAGT ACCGCAAGAC AGACGCCTTC 
CGCCGCCGCC GCTGGCGCCG CCGCATGGAG CCCCTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTCG AGGGGGCACT GGGCGGCGTG GTGGACGACA AGAGTGAAGA TTCCATGTCC 
GCCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACAATTT CCTGCATATT TGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGCGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG CAGCCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTACGGCGC AGACGAGTTT ATGGGCCGCT GCATCTGTCA ACCGAGTTTG 
GAACGGATGC CCCGACTGGC CTGGTTCCCA CTGACCAGGG GCAGCCAGCC GATGGGGGAG 
CTGCTGGCCT CTTTTGAGCT TATCCAGAGA GAGAAGCCAG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC GAGGATCCTG GATGAGTCTG AGGACACAGA CTTGCCCTAC 
CCACCACCCC AAAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATTGAGGT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAGCATCT CCTCCCCGAG CCTTGTGGTA GAGTGTGGGG GCCAAACGGT GCAGTCCTGT 
GTCATTAGGA ACCTCCGGAA GAATCCCAAC TTTGACATCT GTACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCA TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCGTCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGGAGGAAG AGTTTATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACTTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTCATTG GCGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCCACGC AGGGACCGCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGT TGGAGCCTGT GTTTGGAAAG 
ATGTTTGAGC TGACCTGCAC TCTGCCTCTA GAGAAGGACC TGAAGGTCAC CCTCTACGAC 
TATGACCTCC TCTCCAAGGA TGAAAAGATT GGTGAGACGG TCATCGACCT GGAGAACAGG 
CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA CTCCCACAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC 
AGAGTCAAGG CGCCTGTGTA CCGGACAGAC CGTGTGATGT TTCAGGATAA AGAATACTCC 
ATTGAAGAAA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCCAT GGAGGAGCGT 
CTGGCTCTGC ATGTGCTTCG GCAGCAGGGC CTGGTCCCAG AGCACGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATTGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTG 
TTTCCGAAGG CCCTGGGGCG GCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT TATCTGGAAT ACCAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGC TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCGTTT TCCCCTTCGA CTACCTGCCG GCTGAGCAAG TCTGTACGGT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GATATTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCAATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC GAGAAGTGCT CCTTGGACCA GCTGGATGAC 
ACTTACCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCCTGTGTAG CAGAAGAGGG TGAGAAGAAG ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAA CAGAGAGTGA GCATGAGGAG CGGCCCGCTG GCCAGGGCCG GGATGAGCCC 
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCTGACA CCTCCTTCCT GTGGTTCACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTCATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361939.1 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360241.1 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X6, mRNA.

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CloneID	ORh138132
Clone ID Related Accession (Same CDS sequence)	XM_010361939.1
Accession Version	XM_010361939.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-20
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010797358.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rhinopithecus roxellana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGTGGAGGC 
CCAGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCTGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCGGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA AACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTCC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCACCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGAC TGCAGGTGCC 
GTCAAGTCTT CGAAAGCCGC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGTTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGACT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TCGAGGTCAG CATTGGGAAC 
TATGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GTAATGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGCTGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GACCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTTG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACCGGC AAGATCAAGC TACCCAAGGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCAGAGAA GACCCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CTGATGTGAA TGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATACCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GTTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CATCTCGAGT ACCGCAAGAC AGACGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCACT GGGCGGCGTG 
GTGGACGACA AGAGTGAAGA TTCCATGTCC GCCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGCGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTACGGCGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA ACCGAGTTTG GAACGGATGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GATGGGGGAG CTGCTGGCCT CTTTTGAGCT TATCCAGAGA 
GAGAAGCCAG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGTCTG AGGACACAGA CTTGCCCTAC CCACCACCCC AAAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATTGAGGT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAGCATCT CCTCCCCGAG CCTTGTGGTA 
GAGTGTGGGG GCCAAACGGT GCAGTCCTGT GTCATTAGGA ACCTCCGGAA GAATCCCAAC 
TTTGACATCT GTACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCA TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCGTCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC 
CCTACTAACA CGGCTTCTCC TCCATCCAGT CCTCATGAGG AAGAGTTTAT CGATTGGTGG 
AGCAAATTCT TTGCCTCCAT AGGGGAGAGG GAAAAGTGCG GCTCCTACTT GGAGAAGGAT 
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTGTAC CGGGGCAAGA CGCAGGAGGA GACAGAAGAT 
CCATCTGTCA TTGGCGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCA 
GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTGGCCA CGCAGGGACC GCAGGAGTGC 
TTGGTCCGTA TCTACATTGT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCCTGCA CGTTGGAGCC TGTGTTTGGA AAGATGTTTG AGCTGACCTG CACTCTGCCT 
CTAGAGAAGG ACCTGAAGGT CACCCTCTAC GACTATGACC TCCTCTCCAA GGATGAAAAG 
ATTGGTGAGA CGGTCATCGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT 
GGACTCCCAC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGCGGGACCA GCTCCGCCCC 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CGCAGAGTCA AGGCGCCTGT GTACCGGACA 
GACCGTGTGA TGTTTCAGGA TAAAGAATAC TCCATTGAAG AAATAGAGGC TGGCAGGATC 
CCAAACCCAC ACCTGGGCCC CATGGAGGAG CGTCTGGCTC TGCATGTGCT TCGGCAGCAG 
GGCCTGGTCC CAGAGCACGT GGAGTCACGG CCCCTCTACA GCCCCCTGCA GCCAGACATT 
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTGTTTCCGA AGGCCCTGGG GCGGCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATTATCTGG 
AATACCAGAG ATGTGATCCT GGATGACCTG AGCCTCACGG GGGAGAAGAT GAGCGACATT 
TATGTGAAAG GTTGGATGAT TGGCTTTGAA GAACACAAGC AAAAGACAGA CGTGCATTAT 
CGCTCCCTGG GAGGTGAAGG CAACTTCAAC TGGAGGTTCG TTTTCCCCTT CGACTACCTG 
CCGGCTGAGC AAGTCTGTAC GGTTGCCAAG AAGGATGCCT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACG AGTGATATTC CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GATGATTTTC TGGGCTCCCT GCAGCTCAAT CTCAACCGCA TGCCCAAGCC AGCCAAGACA 
GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTACC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAAACAGT GAAGGGCTGG TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG 
AAGATACTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TAACAGAGAG TGAGCATGAG 
GAGCGGCCCG CTGGCCAGGG CCGGGATGAG CCCAACATGA ACCCTAAGCT TGAGGACCCA 
AGGCGCCCTG ACACCTCCTT CCTGTGGTTC ACCTCCCCAT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GTTTCCGGTG GGCCATCATC CTCTTCATCA TCCTCTTCAT CCTGCTGCTG 
TTCCTGGCCA TCTTCATCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360241.1
CDS	136..6444
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEEETAGAVKSSKAADLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLNLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361939.1
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361939.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGTGGAGGC 
CCAGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCTGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCGGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA AACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTCC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCACCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGAC TGCAGGTGCC 
GTCAAGTCTT CGAAAGCCGC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGTTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGACT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TCGAGGTCAG CATTGGGAAC 
TATGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GTAATGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGCTGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GACCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTTG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACCGGC AAGATCAAGC TACCCAAGGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCAGAGAA GACCCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CTGATGTGAA TGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATACCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GTTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CATCTCGAGT ACCGCAAGAC AGACGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCACT GGGCGGCGTG 
GTGGACGACA AGAGTGAAGA TTCCATGTCC GCCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGCGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTACGGCGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA ACCGAGTTTG GAACGGATGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GATGGGGGAG CTGCTGGCCT CTTTTGAGCT TATCCAGAGA 
GAGAAGCCAG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGTCTG AGGACACAGA CTTGCCCTAC CCACCACCCC AAAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATTGAGGT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAGCATCT CCTCCCCGAG CCTTGTGGTA 
GAGTGTGGGG GCCAAACGGT GCAGTCCTGT GTCATTAGGA ACCTCCGGAA GAATCCCAAC 
TTTGACATCT GTACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCA TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCGTCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC 
CCTACTAACA CGGCTTCTCC TCCATCCAGT CCTCATGAGG AAGAGTTTAT CGATTGGTGG 
AGCAAATTCT TTGCCTCCAT AGGGGAGAGG GAAAAGTGCG GCTCCTACTT GGAGAAGGAT 
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTGTAC CGGGGCAAGA CGCAGGAGGA GACAGAAGAT 
CCATCTGTCA TTGGCGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCA 
GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTGGCCA CGCAGGGACC GCAGGAGTGC 
TTGGTCCGTA TCTACATTGT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCCTGCA CGTTGGAGCC TGTGTTTGGA AAGATGTTTG AGCTGACCTG CACTCTGCCT 
CTAGAGAAGG ACCTGAAGGT CACCCTCTAC GACTATGACC TCCTCTCCAA GGATGAAAAG 
ATTGGTGAGA CGGTCATCGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT 
GGACTCCCAC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGCGGGACCA GCTCCGCCCC 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CGCAGAGTCA AGGCGCCTGT GTACCGGACA 
GACCGTGTGA TGTTTCAGGA TAAAGAATAC TCCATTGAAG AAATAGAGGC TGGCAGGATC 
CCAAACCCAC ACCTGGGCCC CATGGAGGAG CGTCTGGCTC TGCATGTGCT TCGGCAGCAG 
GGCCTGGTCC CAGAGCACGT GGAGTCACGG CCCCTCTACA GCCCCCTGCA GCCAGACATT 
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTGTTTCCGA AGGCCCTGGG GCGGCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATTATCTGG 
AATACCAGAG ATGTGATCCT GGATGACCTG AGCCTCACGG GGGAGAAGAT GAGCGACATT 
TATGTGAAAG GTTGGATGAT TGGCTTTGAA GAACACAAGC AAAAGACAGA CGTGCATTAT 
CGCTCCCTGG GAGGTGAAGG CAACTTCAAC TGGAGGTTCG TTTTCCCCTT CGACTACCTG 
CCGGCTGAGC AAGTCTGTAC GGTTGCCAAG AAGGATGCCT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACG AGTGATATTC CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GATGATTTTC TGGGCTCCCT GCAGCTCAAT CTCAACCGCA TGCCCAAGCC AGCCAAGACA 
GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTACC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAAACAGT GAAGGGCTGG TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG 
AAGATACTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TAACAGAGAG TGAGCATGAG 
GAGCGGCCCG CTGGCCAGGG CCGGGATGAG CCCAACATGA ACCCTAAGCT TGAGGACCCA 
AGGCGCCCTG ACACCTCCTT CCTGTGGTTC ACCTCCCCAT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GTTTCCGGTG GGCCATCATC CTCTTCATCA TCCTCTTCAT CCTGCTGCTG 
TTCCTGGCCA TCTTCATCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361940.1 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360242.1 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X7, mRNA.

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CloneID	ORh138133
Clone ID Related Accession (Same CDS sequence)	XM_010361940.1
Accession Version	XM_010361940.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-20
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010797358.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rhinopithecus roxellana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG TGGAGGCCCA 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCTGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCGGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAAAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTCCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CACCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGACTGC AGGTGCCGTC 
AAGTCTTCGA AAGCCGCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GTTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGACTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTCG AGGTCAGCAT TGGGAACTAT 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGTA ATGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGCTGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGAC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTTGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACCGGCAAG ATCAAGCTAC CCAAGGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CAGAGAAGAC CCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACTG ATGTGAATGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATACCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGTT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAT 
CTCGAGTACC GCAAGACAGA CGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCACTGGG CGGCGTGGTG 
GACGACAAGA GTGAAGATTC CATGTCCGCC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GCGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTCGGCGAGC CGGCCACAGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ACGGCGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAACC GAGTTTGGAA CGGATGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAT GGGGGAGCTG CTGGCCTCTT TTGAGCTTAT CCAGAGAGAG 
AAGCCAGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACTT GCCCTACCCA CCACCCCAAA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TTGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGGTAGAG 
TGTGGGGGCC AAACGGTGCA GTCCTGTGTC ATTAGGAACC TCCGGAAGAA TCCCAACTTT 
GACATCTGTA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCGTCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCT 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATGAGGAAG AGTTTATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GCGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCACGC AGGGACCGCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGT TGGAGCCTGT GTTTGGAAAG ATGTTTGAGC TGACCTGCAC TCTGCCTCTA 
GAGAAGGACC TGAAGGTCAC CCTCTACGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGTGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAGG CGCCTGTGTA CCGGACAGAC 
CGTGTGATGT TTCAGGATAA AGAATACTCC ATTGAAGAAA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCCAT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCG GCAGCAGGGC 
CTGGTCCCAG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCGTTT TCCCCTTCGA CTACCTGCCG 
GCTGAGCAAG TCTGTACGGT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GATATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCAATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTACCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCCGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCTGACA CCTCCTTCCT GTGGTTCACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360242.1
CDS	792..7097
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEEETAGAVKSSKAADLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLNLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361940.1
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361940.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG TGGAGGCCCA 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCTGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCGGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAAAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTCCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CACCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGACTGC AGGTGCCGTC 
AAGTCTTCGA AAGCCGCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GTTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGACTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTCG AGGTCAGCAT TGGGAACTAT 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGTA ATGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGCTGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGAC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTTGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACCGGCAAG ATCAAGCTAC CCAAGGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CAGAGAAGAC CCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACTG ATGTGAATGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATACCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGTT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAT 
CTCGAGTACC GCAAGACAGA CGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCACTGGG CGGCGTGGTG 
GACGACAAGA GTGAAGATTC CATGTCCGCC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GCGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTCGGCGAGC CGGCCACAGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ACGGCGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAACC GAGTTTGGAA CGGATGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAT GGGGGAGCTG CTGGCCTCTT TTGAGCTTAT CCAGAGAGAG 
AAGCCAGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACTT GCCCTACCCA CCACCCCAAA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TTGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGGTAGAG 
TGTGGGGGCC AAACGGTGCA GTCCTGTGTC ATTAGGAACC TCCGGAAGAA TCCCAACTTT 
GACATCTGTA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCGTCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCT 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATGAGGAAG AGTTTATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GCGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCACGC AGGGACCGCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGT TGGAGCCTGT GTTTGGAAAG ATGTTTGAGC TGACCTGCAC TCTGCCTCTA 
GAGAAGGACC TGAAGGTCAC CCTCTACGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGTGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAGG CGCCTGTGTA CCGGACAGAC 
CGTGTGATGT TTCAGGATAA AGAATACTCC ATTGAAGAAA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCCAT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCG GCAGCAGGGC 
CTGGTCCCAG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCGTTT TCCCCTTCGA CTACCTGCCG 
GCTGAGCAAG TCTGTACGGT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GATATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCAATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTACCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCCGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCTGACA CCTCCTTCCT GTGGTTCACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361941.1 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360243.1 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X8, mRNA.

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CloneID	ORh138134
Clone ID Related Accession (Same CDS sequence)	XM_010361941.1
Accession Version	XM_010361941.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-20
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010797358.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rhinopithecus roxellana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGTGGA GGCCCAGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCT GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCG 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAAACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TCCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCACC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGTTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGAC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTCGAGGTCA GCATTGGGAA CTATGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGTAATGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGCTG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGACCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTT 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACCGG CAAGATCAAG CTACCCAAGG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCAGAGA AGACCCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACTGATGTGA ATGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATACC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGTTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCATCTCGAG 
TACCGCAAGA CAGACGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCAC TGGGCGGCGT GGTGGACGAC 
AAGAGTGAAG ATTCCATGTC CGCCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGCGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTCGGC GAGCCGGCCA CAGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTACGGCG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AACCGAGTTT GGAACGGATG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGATGGGGGA GCTGCTGGCC TCTTTTGAGC TTATCCAGAG AGAGAAGCCA 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGTCT 
GAGGACACAG ACTTGCCCTA CCCACCACCC CAAAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATTGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGGT AGAGTGTGGG 
GGCCAAACGG TGCAGTCCTG TGTCATTAGG AACCTCCGGA AGAATCCCAA CTTTGACATC 
TGTACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCGTC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCACG CAGGGACCGC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT AGAGAAGGAC 
CTGAAGGTCA CCCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCA TGGAGGAGCG TCTGGCTCTG CATGTGCTTC GGCAGCAGGG CCTGGTCCCA 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCGTT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACGG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCAATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTACCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCTGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360243.1
CDS	136..6432
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLNLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361941.1
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361941.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGTGGA GGCCCAGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCT GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCG 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAAACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TCCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCACC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGTTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGAC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTCGAGGTCA GCATTGGGAA CTATGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGTAATGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGCTG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGACCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTT 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACCGG CAAGATCAAG CTACCCAAGG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCAGAGA AGACCCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACTGATGTGA ATGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATACC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGTTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCATCTCGAG 
TACCGCAAGA CAGACGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCAC TGGGCGGCGT GGTGGACGAC 
AAGAGTGAAG ATTCCATGTC CGCCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGCGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTCGGC GAGCCGGCCA CAGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTACGGCG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AACCGAGTTT GGAACGGATG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGATGGGGGA GCTGCTGGCC TCTTTTGAGC TTATCCAGAG AGAGAAGCCA 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGTCT 
GAGGACACAG ACTTGCCCTA CCCACCACCC CAAAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATTGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGGT AGAGTGTGGG 
GGCCAAACGG TGCAGTCCTG TGTCATTAGG AACCTCCGGA AGAATCCCAA CTTTGACATC 
TGTACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCGTC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCACG CAGGGACCGC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT AGAGAAGGAC 
CTGAAGGTCA CCCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCA TGGAGGAGCG TCTGGCTCTG CATGTGCTTC GGCAGCAGGG CCTGGTCCCA 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCGTT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACGG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCAATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTACCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCTGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361942.1 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360244.1 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X9, mRNA.

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CloneID	ORh138135
Clone ID Related Accession (Same CDS sequence)	XM_010361942.1
Accession Version	XM_010361942.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-20
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010797358.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rhinopithecus roxellana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGTGGAGGC CCAGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCTGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCGGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA AACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTCC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCACCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGTTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGACTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTC GAGGTCAGCA TTGGGAACTA TGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AATGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGCTGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGA CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTTGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACCGGCAA GATCAAGCTA CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCAGAGAAGA CCCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACT GATGTGAATG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATACCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGT TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA TCTCGAGTAC 
CGCAAGACAG ACGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCACTGG GCGGCGTGGT GGACGACAAG 
AGTGAAGATT CCATGTCCGC CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGCGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTCGGCGAG CCGGCCACAG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TACGGCGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAAC CGAGTTTGGA ACGGATGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA TGGGGGAGCT GCTGGCCTCT TTTGAGCTTA TCCAGAGAGA GAAGCCAGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACT TGCCCTACCC ACCACCCCAA AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATTGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC 
CAAACGGTGC AGTCCTGTGT CATTAGGAAC CTCCGGAAGA ATCCCAACTT TGACATCTGT 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCGTCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GGAGGAAGAG TTTATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTCATTGGC 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCACGCAG GGACCGCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGTTG 
GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG ACCTGCACTC TGCCTCTAGA GAAGGACCTG 
AAGGTCACCC TCTACGACTA TGACCTCCTC TCCAAGGATG AAAAGATTGG TGAGACGGTC 
ATCGACCTGG AGAACAGGCT GCTGTCCAAG TTTGCGGCTC GCTGTGGACT CCCACAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG CCTGTGTACC GGACAGACCG TGTGATGTTT 
CAGGATAAAG AATACTCCAT TGAAGAAATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCCATGG AGGAGCGTCT GGCTCTGCAT GTGCTTCGGC AGCAGGGCCT GGTCCCAGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATTGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTGTT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGCTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCGTTTTC CCCTTCGACT ACCTGCCGGC TGAGCAAGTC 
TGTACGGTTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGA TATTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCAATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGACAC TTACCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAACA GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCTGACACC 
TCCTTCCTGT GGTTCACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360244.1
CDS	792..7085
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLNLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361942.1
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361942.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGTGGAGGC CCAGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCTGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCGGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA AACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTCC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCACCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGTTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGACTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTC GAGGTCAGCA TTGGGAACTA TGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AATGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGCTGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGA CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTTGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACCGGCAA GATCAAGCTA CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCAGAGAAGA CCCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACT GATGTGAATG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATACCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGT TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA TCTCGAGTAC 
CGCAAGACAG ACGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCACTGG GCGGCGTGGT GGACGACAAG 
AGTGAAGATT CCATGTCCGC CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGCGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTCGGCGAG CCGGCCACAG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TACGGCGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAAC CGAGTTTGGA ACGGATGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA TGGGGGAGCT GCTGGCCTCT TTTGAGCTTA TCCAGAGAGA GAAGCCAGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACT TGCCCTACCC ACCACCCCAA AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATTGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC 
CAAACGGTGC AGTCCTGTGT CATTAGGAAC CTCCGGAAGA ATCCCAACTT TGACATCTGT 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCGTCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GGAGGAAGAG TTTATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTCATTGGC 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCACGCAG GGACCGCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGTTG 
GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG ACCTGCACTC TGCCTCTAGA GAAGGACCTG 
AAGGTCACCC TCTACGACTA TGACCTCCTC TCCAAGGATG AAAAGATTGG TGAGACGGTC 
ATCGACCTGG AGAACAGGCT GCTGTCCAAG TTTGCGGCTC GCTGTGGACT CCCACAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG CCTGTGTACC GGACAGACCG TGTGATGTTT 
CAGGATAAAG AATACTCCAT TGAAGAAATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCCATGG AGGAGCGTCT GGCTCTGCAT GTGCTTCGGC AGCAGGGCCT GGTCCCAGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATTGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTGTT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGCTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCGTTTTC CCCTTCGACT ACCTGCCGGC TGAGCAAGTC 
TGTACGGTTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGA TATTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCAATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGACAC TTACCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAACA GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCTGACACC 
TCCTTCCTGT GGTTCACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361943.1 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360245.1 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X10, mRNA.

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CloneID	ORh138136
Clone ID Related Accession (Same CDS sequence)	XM_010361943.1
Accession Version	XM_010361943.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-20
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010797358.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rhinopithecus roxellana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGTGGAGGC 
CCAGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCTGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCGGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA AACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTCC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCACCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CCACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGTTTCCTGG ACCCCTACGC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGACTTC TGCTTTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC GGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTC 
GAGGTCAGCA TTGGGAACTA TGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ATAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AATGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CACTGGTGGC TCAGCTGATG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAT ACCCTCTGCC 
ACCCACCTGG ACGAGTACCT GTACCGGCTG CGCACCCGTC ACCTGAGCCA AATCACGGAG 
GCTGCCCTGA CCCTGAAGCT TGGCCACAGT GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTTGCT GAGGAGCCCC AGAACAGCCT GCCGGATATC 
ATCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GTTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACCAGACCA AGTTGGCCCT GGTCGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACCGGCAA GATCAAGCTA 
CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCAGAGAAGA CCCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTTGT GGAGGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACT 
GATGTGAATG GGGAGAAGGT GCTTCCCAAA GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATACCCCC GGACCGGAAG CCGAAGCACT GGGTCCCTGT TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAGATG 
GAAGCGCTGA AGAGGCATAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA TCTCGAGTAC CGCAAGACAG ACGCCTTCCG CCGCCGCCGC 
TGGCGCCGCC GCATGGAGCC CCTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG 
GGGGCACTGG GCGGCGTGGT GGACGACAAG AGTGAAGATT CCATGTCCGC CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACAATTTCC TGCATATTTG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGCGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTCGGCGAG 
CCGGCCACAG TTGCTGAGCA GCCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TACGGCGCAG ACGAGTTTAT GGGCCGCTGC ATCTGTCAAC CGAGTTTGGA ACGGATGCCC 
CGACTGGCCT GGTTCCCACT GACCAGGGGC AGCCAGCCGA TGGGGGAGCT GCTGGCCTCT 
TTTGAGCTTA TCCAGAGAGA GAAGCCAGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCGA GGATCCTGGA TGAGTCTGAG GACACAGACT TGCCCTACCC ACCACCCCAA 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATTGAGGTCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGCATCTCC 
TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC CAAACGGTGC AGTCCTGTGT CATTAGGAAC 
CTCCGGAAGA ATCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCATC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCGTCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC TACTAACACG GCTTCTCCTC CATCCAGTCC TCATGAGGAA 
GAGTTTATCG ATTGGTGGAG CAAATTCTTT GCCTCCATAG GGGAGAGGGA AAAGTGCGGC 
TCCTACTTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTGTACCG GGGCAAGACG 
CAGGAGGAGA CAGAAGATCC ATCTGTCATT GGCGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCAGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTGGCCACG 
CAGGGACCGC AGGAGTGCTT GGTCCGTATC TACATTGTCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCCTGCACG TTGGAGCCTG TGTTTGGAAA GATGTTTGAG 
CTGACCTGCA CTCTGCCTCT AGAGAAGGAC CTGAAGGTCA CCCTCTACGA CTATGACCTC 
CTCTCCAAGG ATGAAAAGAT TGGTGAGACG GTCATCGACC TGGAGAACAG GCTGCTGTCC 
AAGTTTGCGG CTCGCTGTGG ACTCCCACAG ACCTACTGTG TCTCTGGACC GAACCAGTGG 
CGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCG CAGAGTCAAG 
GCGCCTGTGT ACCGGACAGA CCGTGTGATG TTTCAGGATA AAGAATACTC CATTGAAGAA 
ATAGAGGCTG GCAGGATCCC AAACCCACAC CTGGGCCCCA TGGAGGAGCG TCTGGCTCTG 
CATGTGCTTC GGCAGCAGGG CCTGGTCCCA GAGCACGTGG AGTCACGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATTGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT GTTTCCGAAG 
GCCCTGGGGC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA TACCAGAGAT GTGATCCTGG ATGACCTGAG CCTCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGATTG GCTTTGAAGA ACACAAGCAA 
AAGACAGACG TGCATTATCG CTCCCTGGGA GGTGAAGGCA ACTTCAACTG GAGGTTCGTT 
TTCCCCTTCG ACTACCTGCC GGCTGAGCAA GTCTGTACGG TTGCCAAGAA GGATGCCTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACGAG TGATATTCCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA TGATTTTCTG GGCTCCCTGC AGCTCAATCT CAACCGCATG 
CCCAAGCCAG CCAAGACAGC CGAGAAGTGC TCCTTGGACC AGCTGGATGA CACTTACCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAAACAGTGA AGGGCTGGTG GCCCTGTGTA 
GCAGAAGAGG GTGAGAAGAA GATACTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTA 
ACAGAGAGTG AGCATGAGGA GCGGCCCGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCTGAC ACCTCCTTCC TGTGGTTCAC CTCCCCATAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGGG CCATCATCCT CTTCATCATC 
CTCTTCATCC TGCTGCTGTT CCTGGCCATC TTCATCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360245.1
CDS	136..6402
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLNLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361943.1
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361943.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGTGGAGGC 
CCAGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCTGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCGGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA AACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTCC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCACCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CCACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGTTTCCTGG ACCCCTACGC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGACTTC TGCTTTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC GGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTC 
GAGGTCAGCA TTGGGAACTA TGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ATAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AATGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CACTGGTGGC TCAGCTGATG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAT ACCCTCTGCC 
ACCCACCTGG ACGAGTACCT GTACCGGCTG CGCACCCGTC ACCTGAGCCA AATCACGGAG 
GCTGCCCTGA CCCTGAAGCT TGGCCACAGT GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTTGCT GAGGAGCCCC AGAACAGCCT GCCGGATATC 
ATCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GTTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACCAGACCA AGTTGGCCCT GGTCGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACCGGCAA GATCAAGCTA 
CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCAGAGAAGA CCCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTTGT GGAGGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACT 
GATGTGAATG GGGAGAAGGT GCTTCCCAAA GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATACCCCC GGACCGGAAG CCGAAGCACT GGGTCCCTGT TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAGATG 
GAAGCGCTGA AGAGGCATAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA TCTCGAGTAC CGCAAGACAG ACGCCTTCCG CCGCCGCCGC 
TGGCGCCGCC GCATGGAGCC CCTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG 
GGGGCACTGG GCGGCGTGGT GGACGACAAG AGTGAAGATT CCATGTCCGC CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACAATTTCC TGCATATTTG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGCGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTCGGCGAG 
CCGGCCACAG TTGCTGAGCA GCCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TACGGCGCAG ACGAGTTTAT GGGCCGCTGC ATCTGTCAAC CGAGTTTGGA ACGGATGCCC 
CGACTGGCCT GGTTCCCACT GACCAGGGGC AGCCAGCCGA TGGGGGAGCT GCTGGCCTCT 
TTTGAGCTTA TCCAGAGAGA GAAGCCAGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCGA GGATCCTGGA TGAGTCTGAG GACACAGACT TGCCCTACCC ACCACCCCAA 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATTGAGGTCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGCATCTCC 
TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC CAAACGGTGC AGTCCTGTGT CATTAGGAAC 
CTCCGGAAGA ATCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCATC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCGTCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC TACTAACACG GCTTCTCCTC CATCCAGTCC TCATGAGGAA 
GAGTTTATCG ATTGGTGGAG CAAATTCTTT GCCTCCATAG GGGAGAGGGA AAAGTGCGGC 
TCCTACTTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTGTACCG GGGCAAGACG 
CAGGAGGAGA CAGAAGATCC ATCTGTCATT GGCGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCAGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTGGCCACG 
CAGGGACCGC AGGAGTGCTT GGTCCGTATC TACATTGTCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCCTGCACG TTGGAGCCTG TGTTTGGAAA GATGTTTGAG 
CTGACCTGCA CTCTGCCTCT AGAGAAGGAC CTGAAGGTCA CCCTCTACGA CTATGACCTC 
CTCTCCAAGG ATGAAAAGAT TGGTGAGACG GTCATCGACC TGGAGAACAG GCTGCTGTCC 
AAGTTTGCGG CTCGCTGTGG ACTCCCACAG ACCTACTGTG TCTCTGGACC GAACCAGTGG 
CGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCG CAGAGTCAAG 
GCGCCTGTGT ACCGGACAGA CCGTGTGATG TTTCAGGATA AAGAATACTC CATTGAAGAA 
ATAGAGGCTG GCAGGATCCC AAACCCACAC CTGGGCCCCA TGGAGGAGCG TCTGGCTCTG 
CATGTGCTTC GGCAGCAGGG CCTGGTCCCA GAGCACGTGG AGTCACGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATTGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT GTTTCCGAAG 
GCCCTGGGGC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA TACCAGAGAT GTGATCCTGG ATGACCTGAG CCTCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGATTG GCTTTGAAGA ACACAAGCAA 
AAGACAGACG TGCATTATCG CTCCCTGGGA GGTGAAGGCA ACTTCAACTG GAGGTTCGTT 
TTCCCCTTCG ACTACCTGCC GGCTGAGCAA GTCTGTACGG TTGCCAAGAA GGATGCCTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACGAG TGATATTCCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA TGATTTTCTG GGCTCCCTGC AGCTCAATCT CAACCGCATG 
CCCAAGCCAG CCAAGACAGC CGAGAAGTGC TCCTTGGACC AGCTGGATGA CACTTACCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAAACAGTGA AGGGCTGGTG GCCCTGTGTA 
GCAGAAGAGG GTGAGAAGAA GATACTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTA 
ACAGAGAGTG AGCATGAGGA GCGGCCCGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCTGAC ACCTCCTTCC TGTGGTTCAC CTCCCCATAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGGG CCATCATCCT CTTCATCATC 
CTCTTCATCC TGCTGCTGTT CCTGGCCATC TTCATCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361944.1 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360246.1 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X11, mRNA.

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CloneID	ORh138137
Clone ID Related Accession (Same CDS sequence)	XM_010361944.1
Accession Version	XM_010361944.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-20
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010797358.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rhinopithecus roxellana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG TGGAGGCCCA 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCTGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCGGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAAAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTCCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CACCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACCA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGT 
TTCCTGGACC CCTACGCAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGACTTCTGC TTTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAGCAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCGGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTCGAG 
GTCAGCATTG GGAACTATGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTATA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGTAAT 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCAC TGGTGGCTCA GCTGATGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGATACC CTCTGCCACC 
CACCTGGACG AGTACCTGTA CCGGCTGCGC ACCCGTCACC TGAGCCAAAT CACGGAGGCT 
GCCCTGACCC TGAAGCTTGG CCACAGTGAG CTCCCTGCTG CTCTGGATCA GGCTGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTTGCTGAG GAGCCCCAGA ACAGCCTGCC GGATATCATC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGTT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC CAGACCAAGT TGGCCCTGGT CGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CCGGCAAGAT CAAGCTACCC 
AAGGAGAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCA 
GAGAAGACCC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTTGTGGA GGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACTGAT 
GTGAATGGGG AGAAGGTGCT TCCCAAAGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TACCCCCGGA CCGGAAGCCG AAGCACTGGG TCCCTGTTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAGATGGAA 
GCGCTGAAGA GGCATAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCATCT CGAGTACCGC AAGACAGACG CCTTCCGCCG CCGCCGCTGG 
CGCCGCCGCA TGGAGCCCCT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTCGAGGGG 
GCACTGGGCG GCGTGGTGGA CGACAAGAGT GAAGATTCCA TGTCCGCCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC AATTTCCTGC ATATTTGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGC GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT CGGCGAGCCG 
GCCACAGTTG CTGAGCAGCC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAC 
GGCGCAGACG AGTTTATGGG CCGCTGCATC TGTCAACCGA GTTTGGAACG GATGCCCCGA 
CTGGCCTGGT TCCCACTGAC CAGGGGCAGC CAGCCGATGG GGGAGCTGCT GGCCTCTTTT 
GAGCTTATCC AGAGAGAGAA GCCAGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCGAGGA TCCTGGATGA GTCTGAGGAC ACAGACTTGC CCTACCCACC ACCCCAAAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGGTAGAGTG TGGGGGCCAA ACGGTGCAGT CCTGTGTCAT TAGGAACCTC 
CGGAAGAATC CCAACTTTGA CATCTGTACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CGTCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCTAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCACGCAG 
GGACCGCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTAGA GAAGGACCTG AAGGTCACCC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCATGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCGGC AGCAGGGCCT GGTCCCAGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCGTTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACGGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCAATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTACCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCTGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360246.1
CDS	792..7055
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLNLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361944.1
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361944.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG TGGAGGCCCA 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCTGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCGGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAAAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTCCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CACCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACCA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGT 
TTCCTGGACC CCTACGCAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGACTTCTGC TTTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAGCAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCGGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTCGAG 
GTCAGCATTG GGAACTATGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTATA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGTAAT 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCAC TGGTGGCTCA GCTGATGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGATACC CTCTGCCACC 
CACCTGGACG AGTACCTGTA CCGGCTGCGC ACCCGTCACC TGAGCCAAAT CACGGAGGCT 
GCCCTGACCC TGAAGCTTGG CCACAGTGAG CTCCCTGCTG CTCTGGATCA GGCTGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTTGCTGAG GAGCCCCAGA ACAGCCTGCC GGATATCATC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGTT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC CAGACCAAGT TGGCCCTGGT CGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CCGGCAAGAT CAAGCTACCC 
AAGGAGAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCA 
GAGAAGACCC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTTGTGGA GGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACTGAT 
GTGAATGGGG AGAAGGTGCT TCCCAAAGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TACCCCCGGA CCGGAAGCCG AAGCACTGGG TCCCTGTTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAGATGGAA 
GCGCTGAAGA GGCATAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCATCT CGAGTACCGC AAGACAGACG CCTTCCGCCG CCGCCGCTGG 
CGCCGCCGCA TGGAGCCCCT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTCGAGGGG 
GCACTGGGCG GCGTGGTGGA CGACAAGAGT GAAGATTCCA TGTCCGCCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC AATTTCCTGC ATATTTGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGC GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT CGGCGAGCCG 
GCCACAGTTG CTGAGCAGCC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAC 
GGCGCAGACG AGTTTATGGG CCGCTGCATC TGTCAACCGA GTTTGGAACG GATGCCCCGA 
CTGGCCTGGT TCCCACTGAC CAGGGGCAGC CAGCCGATGG GGGAGCTGCT GGCCTCTTTT 
GAGCTTATCC AGAGAGAGAA GCCAGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCGAGGA TCCTGGATGA GTCTGAGGAC ACAGACTTGC CCTACCCACC ACCCCAAAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGGTAGAGTG TGGGGGCCAA ACGGTGCAGT CCTGTGTCAT TAGGAACCTC 
CGGAAGAATC CCAACTTTGA CATCTGTACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CGTCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCTAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCACGCAG 
GGACCGCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTAGA GAAGGACCTG AAGGTCACCC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCATGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCGGC AGCAGGGCCT GGTCCCAGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCGTTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACGGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCAATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTACCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCTGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361945.1 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360247.1 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X12, mRNA.

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CloneID	ORh138138
Clone ID Related Accession (Same CDS sequence)	XM_010361945.1
Accession Version	XM_010361945.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-20
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010797358.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rhinopithecus roxellana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGTGGAGGC 
CCAGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCTGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCGGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA AACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTCC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCACCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGAC TGCAGGTGCC 
GTCAAGTCTT CGAAAGCCGC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGTTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGACT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TCGAGGTCAG CATTGGGAAC 
TATGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GTAATGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGCTGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GACCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTTG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACCGGC AAGATCAAGC TACCCAAGGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCAGAGAA GACCCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CTGATGTGAA TGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATACCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GTTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CATCTCGAGT ACCGCAAGAC AGACGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCACT GGGCGGCGTG 
GTGGACGACA AGAGTGAAGA TTCCATGTCC GCCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGCGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTACGGCGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA ACCGAGTTTG GAACGGATGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GATGGGGGAG CTGCTGGCCT CTTTTGAGCT TATCCAGAGA 
GAGAAGCCAG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGTCTG AGGACACAGA CTTGCCCTAC CCACCACCCC AAAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATTGAGGT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAGCATCT CCTCCCCGAG CCTTGTGGTA 
GAGTGTGGGG GCCAAACGGT GCAGTCCTGT GTCATTAGGA ACCTCCGGAA GAATCCCAAC 
TTTGACATCT GTACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCA TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCGTCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGGAGGAAG AGTTTATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GCGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCACGC AGGGACCGCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGT TGGAGCCTGT GTTTGGAAAG ATGTTTGAGC TGACCTGCAC TCTGCCTCTA 
GAGAAGGACC TGAAGGTCAC CCTCTACGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGTGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAGG CGCCTGTGTA CCGGACAGAC 
CGTGTGATGT TTCAGGATAA AGAATACTCC ATTGAAGAAA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCCAT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCG GCAGCAGGGC 
CTGGTCCCAG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCGTTT TCCCCTTCGA CTACCTGCCG 
GCTGAGCAAG TCTGTACGGT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GATATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCAATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTACCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCCGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCTGACA CCTCCTTCCT GTGGTTCACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360247.1
CDS	136..6381
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEEETAGAVKSSKAADLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLNLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361945.1
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361945.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGTGGAGGC 
CCAGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCTGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCGGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA AACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTCC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCACCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGAC TGCAGGTGCC 
GTCAAGTCTT CGAAAGCCGC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGTTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGACT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TCGAGGTCAG CATTGGGAAC 
TATGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GTAATGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGCTGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GACCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTTG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACCGGC AAGATCAAGC TACCCAAGGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCAGAGAA GACCCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CTGATGTGAA TGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATACCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GTTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CATCTCGAGT ACCGCAAGAC AGACGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCACT GGGCGGCGTG 
GTGGACGACA AGAGTGAAGA TTCCATGTCC GCCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGCGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTACGGCGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA ACCGAGTTTG GAACGGATGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GATGGGGGAG CTGCTGGCCT CTTTTGAGCT TATCCAGAGA 
GAGAAGCCAG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGTCTG AGGACACAGA CTTGCCCTAC CCACCACCCC AAAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATTGAGGT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAGCATCT CCTCCCCGAG CCTTGTGGTA 
GAGTGTGGGG GCCAAACGGT GCAGTCCTGT GTCATTAGGA ACCTCCGGAA GAATCCCAAC 
TTTGACATCT GTACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCA TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCGTCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGGAGGAAG AGTTTATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GCGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCACGC AGGGACCGCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGT TGGAGCCTGT GTTTGGAAAG ATGTTTGAGC TGACCTGCAC TCTGCCTCTA 
GAGAAGGACC TGAAGGTCAC CCTCTACGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGTGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAGG CGCCTGTGTA CCGGACAGAC 
CGTGTGATGT TTCAGGATAA AGAATACTCC ATTGAAGAAA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCCAT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCG GCAGCAGGGC 
CTGGTCCCAG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCGTTT TCCCCTTCGA CTACCTGCCG 
GCTGAGCAAG TCTGTACGGT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GATATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCAATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTACCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCCGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCTGACA CCTCCTTCCT GTGGTTCACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361946.1 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360248.1 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X13, mRNA.

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CloneID	ORh138139
Clone ID Related Accession (Same CDS sequence)	XM_010361946.1
Accession Version	XM_010361946.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-20
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010797358.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rhinopithecus roxellana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG TGGAGGCCCA 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCTGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCGGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAAAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTCCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CACCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGACTGC AGGTGCCGTC 
AAGTCTTCGA AAGCCGCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GTTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGACTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTCG AGGTCAGCAT TGGGAACTAT 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGTA ATGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGCTGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGAC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTTGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACCGGCAAG ATCAAGCTAC CCAAGGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CAGAGAAGAC CCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACTG ATGTGAATGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATACCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGTT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAT 
CTCGAGTACC GCAAGACAGA CGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCACTGGG CGGCGTGGTG 
GACGACAAGA GTGAAGATTC CATGTCCGCC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GCGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTCGGCGAGC CGGCCACAGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ACGGCGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAACC GAGTTTGGAA CGGATGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAT GGGGGAGCTG CTGGCCTCTT TTGAGCTTAT CCAGAGAGAG 
AAGCCAGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACTT GCCCTACCCA CCACCCCAAA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TTGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGGTAGAG 
TGTGGGGGCC AAACGGTGCA GTCCTGTGTC ATTAGGAACC TCCGGAAGAA TCCCAACTTT 
GACATCTGTA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCGTCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG GAGGAAGAGT TTATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACTTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTCATTGGCG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCACGCAGG GACCGCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGTTGG AGCCTGTGTT TGGAAAGATG TTTGAGCTGA CCTGCACTCT GCCTCTAGAG 
AAGGACCTGA AGGTCACCCT CTACGACTAT GACCTCCTCT CCAAGGATGA AAAGATTGGT 
GAGACGGTCA TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGCGGCTCG CTGTGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCGCAGA GTCAAGGCGC CTGTGTACCG GACAGACCGT 
GTGATGTTTC AGGATAAAGA ATACTCCATT GAAGAAATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCCATGGA GGAGCGTCTG GCTCTGCATG TGCTTCGGCA GCAGGGCCTG 
GTCCCAGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATTGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTGTTT CCGAAGGCCC TGGGGCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACC 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGCTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCGTTTTCC CCTTCGACTA CCTGCCGGCT 
GAGCAAGTCT GTACGGTTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGAT ATTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CAATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACT TACCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTAGCAG AAGAGGGTGA GAAGAAGATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAACAG AGAGTGAGCA TGAGGAGCGG 
CCCGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCTGACACCT CCTTCCTGTG GTTCACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360248.1
CDS	792..7034
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEEETAGAVKSSKAADLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLNLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361946.1
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361946.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG TGGAGGCCCA 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCTGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCGGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAAAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTCCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CACCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGACTGC AGGTGCCGTC 
AAGTCTTCGA AAGCCGCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GTTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGACTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTCG AGGTCAGCAT TGGGAACTAT 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGTA ATGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGCTGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGAC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTTGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACCGGCAAG ATCAAGCTAC CCAAGGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CAGAGAAGAC CCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACTG ATGTGAATGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATACCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGTT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAT 
CTCGAGTACC GCAAGACAGA CGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCACTGGG CGGCGTGGTG 
GACGACAAGA GTGAAGATTC CATGTCCGCC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GCGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTCGGCGAGC CGGCCACAGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ACGGCGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAACC GAGTTTGGAA CGGATGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAT GGGGGAGCTG CTGGCCTCTT TTGAGCTTAT CCAGAGAGAG 
AAGCCAGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACTT GCCCTACCCA CCACCCCAAA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TTGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGGTAGAG 
TGTGGGGGCC AAACGGTGCA GTCCTGTGTC ATTAGGAACC TCCGGAAGAA TCCCAACTTT 
GACATCTGTA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCGTCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG GAGGAAGAGT TTATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACTTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTCATTGGCG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCACGCAGG GACCGCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGTTGG AGCCTGTGTT TGGAAAGATG TTTGAGCTGA CCTGCACTCT GCCTCTAGAG 
AAGGACCTGA AGGTCACCCT CTACGACTAT GACCTCCTCT CCAAGGATGA AAAGATTGGT 
GAGACGGTCA TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGCGGCTCG CTGTGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCGCAGA GTCAAGGCGC CTGTGTACCG GACAGACCGT 
GTGATGTTTC AGGATAAAGA ATACTCCATT GAAGAAATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCCATGGA GGAGCGTCTG GCTCTGCATG TGCTTCGGCA GCAGGGCCTG 
GTCCCAGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATTGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTGTTT CCGAAGGCCC TGGGGCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACC 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGCTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCGTTTTCC CCTTCGACTA CCTGCCGGCT 
GAGCAAGTCT GTACGGTTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGAT ATTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CAATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACT TACCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTAGCAG AAGAGGGTGA GAAGAAGATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAACAG AGAGTGAGCA TGAGGAGCGG 
CCCGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCTGACACCT CCTTCCTGTG GTTCACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361947.1 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360249.1 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X14, mRNA.

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CloneID	ORh138140
Clone ID Related Accession (Same CDS sequence)	XM_010361947.1
Accession Version	XM_010361947.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-20
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010797358.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rhinopithecus roxellana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGTGGAGGC 
CCAGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCTGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCGGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA AACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTCC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCACCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CCACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGTTTCCTGG ACCCCTACGC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGACTTC TGCTTTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC GGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTC 
GAGGTCAGCA TTGGGAACTA TGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ATAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AATGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CACTGGTGGC TCAGCTGATG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAT ACCCTCTGCC 
ACCCACCTGG ACGAGTACCT GTACCGGCTG CGCACCCGTC ACCTGAGCCA AATCACGGAG 
GCTGCCCTGA CCCTGAAGCT TGGCCACAGT GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTTGCT GAGGAGCCCC AGAACAGCCT GCCGGATATC 
ATCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GTTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACCAGACCA AGTTGGCCCT GGTCGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACCGGCAA GATCAAGCTA 
CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCAGAGAAGA CCCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTTGT GGAGGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACT 
GATGTGAATG GGGAGAAGGT GCTTCCCAAA GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATACCCCC GGACCGGAAG CCGAAGCACT GGGTCCCTGT TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAGATG 
GAAGCGCTGA AGAGGCATAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA TCTCGAGTAC CGCAAGACAG ACGCCTTCCG CCGCCGCCGC 
TGGCGCCGCC GCATGGAGCC CCTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG 
GGGGCACTGG GCGGCGTGGT GGACGACAAG AGTGAAGATT CCATGTCCGC CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACAATTTCC TGCATATTTG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGCGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTCGGCGAG 
CCGGCCACAG TTGCTGAGCA GCCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TACGGCGCAG ACGAGTTTAT GGGCCGCTGC ATCTGTCAAC CGAGTTTGGA ACGGATGCCC 
CGACTGGCCT GGTTCCCACT GACCAGGGGC AGCCAGCCGA TGGGGGAGCT GCTGGCCTCT 
TTTGAGCTTA TCCAGAGAGA GAAGCCAGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCGA GGATCCTGGA TGAGTCTGAG GACACAGACT TGCCCTACCC ACCACCCCAA 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATTGAGGTCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGCATCTCC 
TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC CAAACGGTGC AGTCCTGTGT CATTAGGAAC 
CTCCGGAAGA ATCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCATC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCGTCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCACGCAG 
GGACCGCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTAGA GAAGGACCTG AAGGTCACCC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCATGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCGGC AGCAGGGCCT GGTCCCAGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCGTTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACGGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCAATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTACCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCTGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360249.1
CDS	136..6339
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLNLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361947.1
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361947.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGTGGAGGC 
CCAGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCTGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCGGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA AACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTCC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCACCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CCACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGTTTCCTGG ACCCCTACGC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGACTTC TGCTTTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC GGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTC 
GAGGTCAGCA TTGGGAACTA TGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ATAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AATGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CACTGGTGGC TCAGCTGATG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAT ACCCTCTGCC 
ACCCACCTGG ACGAGTACCT GTACCGGCTG CGCACCCGTC ACCTGAGCCA AATCACGGAG 
GCTGCCCTGA CCCTGAAGCT TGGCCACAGT GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTTGCT GAGGAGCCCC AGAACAGCCT GCCGGATATC 
ATCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GTTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACCAGACCA AGTTGGCCCT GGTCGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACCGGCAA GATCAAGCTA 
CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCAGAGAAGA CCCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTTGT GGAGGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACT 
GATGTGAATG GGGAGAAGGT GCTTCCCAAA GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATACCCCC GGACCGGAAG CCGAAGCACT GGGTCCCTGT TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAGATG 
GAAGCGCTGA AGAGGCATAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA TCTCGAGTAC CGCAAGACAG ACGCCTTCCG CCGCCGCCGC 
TGGCGCCGCC GCATGGAGCC CCTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG 
GGGGCACTGG GCGGCGTGGT GGACGACAAG AGTGAAGATT CCATGTCCGC CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACAATTTCC TGCATATTTG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGCGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTCGGCGAG 
CCGGCCACAG TTGCTGAGCA GCCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TACGGCGCAG ACGAGTTTAT GGGCCGCTGC ATCTGTCAAC CGAGTTTGGA ACGGATGCCC 
CGACTGGCCT GGTTCCCACT GACCAGGGGC AGCCAGCCGA TGGGGGAGCT GCTGGCCTCT 
TTTGAGCTTA TCCAGAGAGA GAAGCCAGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCGA GGATCCTGGA TGAGTCTGAG GACACAGACT TGCCCTACCC ACCACCCCAA 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATTGAGGTCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGCATCTCC 
TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC CAAACGGTGC AGTCCTGTGT CATTAGGAAC 
CTCCGGAAGA ATCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCATC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCGTCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCACGCAG 
GGACCGCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTAGA GAAGGACCTG AAGGTCACCC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCATGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCGGC AGCAGGGCCT GGTCCCAGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCGTTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACGGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCAATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTACCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCTGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361948.1 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360250.1 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X15, mRNA.

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CloneID	ORh138141
Clone ID Related Accession (Same CDS sequence)	XM_010361948.1
Accession Version	XM_010361948.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-20
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010797358.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rhinopithecus roxellana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG TGGAGGCCCA 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCTGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCGGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAAAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTCCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CACCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACCA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGT 
TTCCTGGACC CCTACGCAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGACTTCTGC TTTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAGCAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCGGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTCGAG 
GTCAGCATTG GGAACTATGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTATA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGTAAT 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCAC TGGTGGCTCA GCTGATGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGATACC CTCTGCCACC 
CACCTGGACG AGTACCTGTA CCGGCTGCGC ACCCGTCACC TGAGCCAAAT CACGGAGGCT 
GCCCTGACCC TGAAGCTTGG CCACAGTGAG CTCCCTGCTG CTCTGGATCA GGCTGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTTGCTGAG GAGCCCCAGA ACAGCCTGCC GGATATCATC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGTT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC CAGACCAAGT TGGCCCTGGT CGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CCGGCAAGAT CAAGCTACCC 
AAGGAGAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCA 
GAGAAGACCC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTTGTGGA GGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACTGAT 
GTGAATGGGG AGAAGGTGCT TCCCAAAGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TACCCCCGGA CCGGAAGCCG AAGCACTGGG TCCCTGTTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAGATGGAA 
GCGCTGAAGA GGCATAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCATCT CGAGTACCGC AAGACAGACG CCTTCCGCCG CCGCCGCTGG 
CGCCGCCGCA TGGAGCCCCT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTCGAGGGG 
GCACTGGGCG GCGTGGTGGA CGACAAGAGT GAAGATTCCA TGTCCGCCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC AATTTCCTGC ATATTTGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGC GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT CGGCGAGCCG 
GCCACAGTTG CTGAGCAGCC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAC 
GGCGCAGACG AGTTTATGGG CCGCTGCATC TGTCAACCGA GTTTGGAACG GATGCCCCGA 
CTGGCCTGGT TCCCACTGAC CAGGGGCAGC CAGCCGATGG GGGAGCTGCT GGCCTCTTTT 
GAGCTTATCC AGAGAGAGAA GCCAGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCGAGGA TCCTGGATGA GTCTGAGGAC ACAGACTTGC CCTACCCACC ACCCCAAAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGGTAGAGTG TGGGGGCCAA ACGGTGCAGT CCTGTGTCAT TAGGAACCTC 
CGGAAGAATC CCAACTTTGA CATCTGTACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CGTCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTT 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT CATTGGCGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CACGCAGGGA 
CCGCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGTTGGAG CCTGTGTTTG GAAAGATGTT TGAGCTGACC 
TGCACTCTGC CTCTAGAGAA GGACCTGAAG GTCACCCTCT ACGACTATGA CCTCCTCTCC 
AAGGATGAAA AGATTGGTGA GACGGTCATC GACCTGGAGA ACAGGCTGCT GTCCAAGTTT 
GCGGCTCGCT GTGGACTCCC ACAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCGCAGAGT CAAGGCGCCT 
GTGTACCGGA CAGACCGTGT GATGTTTCAG GATAAAGAAT ACTCCATTGA AGAAATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCCATGGAGG AGCGTCTGGC TCTGCATGTG 
CTTCGGCAGC AGGGCCTGGT CCCAGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TTGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTGTTTCC GAAGGCCCTG 
GGGCGGCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TGTATTATCT GGAATACCAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGCTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CGTTTTCCCC 
TTCGACTACC TGCCGGCTGA GCAAGTCTGT ACGGTTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGATAT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCA ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGACACTTA CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCCTG TGTAGCAGAA 
GAGGGTGAGA AGAAGATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAACAGAG 
AGTGAGCATG AGGAGCGGCC CGCTGGCCAG GGCCGGGATG AGCCCAACAT GAACCCTAAG 
CTTGAGGACC CAAGGCGCCC TGACACCTCC TTCCTGTGGT TCACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTCAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360250.1
CDS	792..6992
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLNLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361948.1
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361948.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG TGGAGGCCCA 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCTGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCGGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAAAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTCCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CACCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACCA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGT 
TTCCTGGACC CCTACGCAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGACTTCTGC TTTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAGCAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCGGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTCGAG 
GTCAGCATTG GGAACTATGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTATA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGTAAT 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCAC TGGTGGCTCA GCTGATGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGATACC CTCTGCCACC 
CACCTGGACG AGTACCTGTA CCGGCTGCGC ACCCGTCACC TGAGCCAAAT CACGGAGGCT 
GCCCTGACCC TGAAGCTTGG CCACAGTGAG CTCCCTGCTG CTCTGGATCA GGCTGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTTGCTGAG GAGCCCCAGA ACAGCCTGCC GGATATCATC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGTT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC CAGACCAAGT TGGCCCTGGT CGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CCGGCAAGAT CAAGCTACCC 
AAGGAGAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCA 
GAGAAGACCC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTTGTGGA GGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACTGAT 
GTGAATGGGG AGAAGGTGCT TCCCAAAGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TACCCCCGGA CCGGAAGCCG AAGCACTGGG TCCCTGTTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAGATGGAA 
GCGCTGAAGA GGCATAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCATCT CGAGTACCGC AAGACAGACG CCTTCCGCCG CCGCCGCTGG 
CGCCGCCGCA TGGAGCCCCT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTCGAGGGG 
GCACTGGGCG GCGTGGTGGA CGACAAGAGT GAAGATTCCA TGTCCGCCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC AATTTCCTGC ATATTTGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGC GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT CGGCGAGCCG 
GCCACAGTTG CTGAGCAGCC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAC 
GGCGCAGACG AGTTTATGGG CCGCTGCATC TGTCAACCGA GTTTGGAACG GATGCCCCGA 
CTGGCCTGGT TCCCACTGAC CAGGGGCAGC CAGCCGATGG GGGAGCTGCT GGCCTCTTTT 
GAGCTTATCC AGAGAGAGAA GCCAGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCGAGGA TCCTGGATGA GTCTGAGGAC ACAGACTTGC CCTACCCACC ACCCCAAAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGGTAGAGTG TGGGGGCCAA ACGGTGCAGT CCTGTGTCAT TAGGAACCTC 
CGGAAGAATC CCAACTTTGA CATCTGTACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CGTCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTT 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT CATTGGCGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CACGCAGGGA 
CCGCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGTTGGAG CCTGTGTTTG GAAAGATGTT TGAGCTGACC 
TGCACTCTGC CTCTAGAGAA GGACCTGAAG GTCACCCTCT ACGACTATGA CCTCCTCTCC 
AAGGATGAAA AGATTGGTGA GACGGTCATC GACCTGGAGA ACAGGCTGCT GTCCAAGTTT 
GCGGCTCGCT GTGGACTCCC ACAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCGCAGAGT CAAGGCGCCT 
GTGTACCGGA CAGACCGTGT GATGTTTCAG GATAAAGAAT ACTCCATTGA AGAAATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCCATGGAGG AGCGTCTGGC TCTGCATGTG 
CTTCGGCAGC AGGGCCTGGT CCCAGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TTGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTGTTTCC GAAGGCCCTG 
GGGCGGCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TGTATTATCT GGAATACCAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGCTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CGTTTTCCCC 
TTCGACTACC TGCCGGCTGA GCAAGTCTGT ACGGTTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGATAT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCA ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGACACTTA CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCCTG TGTAGCAGAA 
GAGGGTGAGA AGAAGATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAACAGAG 
AGTGAGCATG AGGAGCGGCC CGCTGGCCAG GGCCGGGATG AGCCCAACAT GAACCCTAAG 
CTTGAGGACC CAAGGCGCCC TGACACCTCC TTCCTGTGGT TCACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTCAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_030921390.1 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_030777250.1 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X1, mRNA.

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CloneID	ORh253189
Clone ID Related Accession (Same CDS sequence)	XM_030921390.1
Accession Version	XM_030921390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Rhinopithecus roxellana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGTGGA GGCCCAGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCT GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCG 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAAACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TCCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCACC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGAGGA GACTGCAGGT GCCGTCAAGT CTTCGAAAGC CGCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGTTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG ACTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTCGAGGT CAGCATTGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGTAATGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGC TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGACCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TTGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA GGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCAGA GAAGACCCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACTGATGT GAATGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATA CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGTTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCATCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC ACTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGCCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGCGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAACCGAGT 
TTGGAACGGA TGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGATGGGG 
GAGCTGCTGG CCTCTTTTGA GCTTATCCAG AGAGAGAAGC CAGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGT CTGAGGACAC AGACTTGCCC 
TACCCACCAC CCCAAAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GGTCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAGCA TCTCCTCCCC GAGCCTTGTG GTAGAGTGTG GGGGCCAAAC GGTGCAGTCC 
TGTGTCATTA GGAACCTCCG GAAGAATCCC AACTTTGACA TCTGTACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCATCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCG TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCTACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATG AGGAAGAGTT TATCGATTGG TGGAGCAAAT TCTTTGCCTC CATAGGGGAG 
AGGGAAAAGT GCGGCTCCTA CTTGGAGAAG GATTTTGACA CCCTGAAGGT CTATGACACA 
CAGCTGGAGA ATGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTG 
TACCGGGGCA AGACGCAGGA GGAGACAGAA GATCCATCTG TCATTGGCGA ATTTAAGGGC 
CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCAGCCATCC CCATGCCCCC AAGACAGTTC 
CACCAGCTGG CCACGCAGGG ACCGCAGGAG TGCTTGGTCC GTATCTACAT TGTCCGAGCA 
TTTGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCCT GCACGTTGGA GCCTGTGTTT 
GGAAAGATGT TTGAGCTGAC CTGCACTCTG CCTCTAGAGA AGGACCTGAA GGTCACCCTC 
TACGACTATG ACCTCCTCTC CAAGGATGAA AAGATTGGTG AGACGGTCAT CGACCTGGAG 
AACAGGCTGC TGTCCAAGTT TGCGGCTCGC TGTGGACTCC CACAGACCTA CTGTGTCTCT 
GGACCGAACC AGTGGCGGGA CCAGCTCCAC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCGCAGAG TCAAGGCGCC TGTGTACCGG ACAGACCGTG TGATGTTTCA GGATAAAGAA 
TACTCCATTG AAGAAATAGA GGCTGGCAGG ATCCCAAACC CACACCTGGG CCCCATGGAG 
GAGCGTCTGG CTCTGCATGT GCTTCGGCAG CAGGGCCTGG TCCCAGAGCA CGTGGAGTCA 
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATTGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTGTTTC CGAAGGCCCT GGGGCGGCCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTGTATTATC TGGAATACCA GAGATGTGAT CCTGGATGAC 
CTGAGCCTCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GATTGGCTTT 
GAAGAACACA AGCAAAAGAC AGACGTGCAT TATCGCTCCC TGGGAGGTGA AGGCAACTTC 
AACTGGAGGT TCGTTTTCCC CTTCGACTAC CTGCCGGCTG AGCAAGTCTG TACGGTTGCC 
AAGAAGGATG CCTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACGAGTGATA 
TTCCAGATCT GGGACAATGA CAAGTTCTCC TTTGATGATT TTCTGGGCTC CCTGCAGCTC 
GATCTCAACC GCATGCCCAA GCCAGCCAAG ACAGCCGAGA AGTGCTCCTT GGACCAGCTG 
GATGACACTT ACCACCCAGA ATGGTTTGTG TCCCTTTTTG AGCAGAAAAC AGTGAAGGGC 
TGGTGGCCCT GTGTAGCAGA AGAGGGTGAG AAGAAGATAC TGGCGGGCAA GCTGGAAATG 
ACCTTGGAGA TTGTAACAGA GAGTGAGCAT GAGGAGCGGC CCGCTGGCCA GGGCCGGGAT 
GAGCCCAACA TGAACCCTAA GCTTGAGGAC CCAAGGCGCC CTGACACCTC CTTCCTGTGG 
TTCACCTCCC CATACAAGAC CATGAAGTTC ATCCTGTGGC GGCGTTTCCG GTGGGCCATC 
ATCCTCTTCA TCATCCTCTT CATCCTGCTG CTGTTCCTGG CCATCTTCAT CTACGCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030777250.1
CDS	106..6507
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEEETAGAVKSSKAADLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLHPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_030921390.1
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030921390.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGTGGA GGCCCAGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCT GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCG 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAAACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TCCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCACC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGAGGA GACTGCAGGT GCCGTCAAGT CTTCGAAAGC CGCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGTTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG ACTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTCGAGGT CAGCATTGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGTAATGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGC TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGACCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TTGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA GGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCAGA GAAGACCCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACTGATGT GAATGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATA CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGTTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCATCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC ACTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGCCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGCGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAACCGAGT 
TTGGAACGGA TGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGATGGGG 
GAGCTGCTGG CCTCTTTTGA GCTTATCCAG AGAGAGAAGC CAGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGT CTGAGGACAC AGACTTGCCC 
TACCCACCAC CCCAAAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GGTCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAGCA TCTCCTCCCC GAGCCTTGTG GTAGAGTGTG GGGGCCAAAC GGTGCAGTCC 
TGTGTCATTA GGAACCTCCG GAAGAATCCC AACTTTGACA TCTGTACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCATCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCG TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCTACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATG AGGAAGAGTT TATCGATTGG TGGAGCAAAT TCTTTGCCTC CATAGGGGAG 
AGGGAAAAGT GCGGCTCCTA CTTGGAGAAG GATTTTGACA CCCTGAAGGT CTATGACACA 
CAGCTGGAGA ATGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTG 
TACCGGGGCA AGACGCAGGA GGAGACAGAA GATCCATCTG TCATTGGCGA ATTTAAGGGC 
CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCAGCCATCC CCATGCCCCC AAGACAGTTC 
CACCAGCTGG CCACGCAGGG ACCGCAGGAG TGCTTGGTCC GTATCTACAT TGTCCGAGCA 
TTTGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCCT GCACGTTGGA GCCTGTGTTT 
GGAAAGATGT TTGAGCTGAC CTGCACTCTG CCTCTAGAGA AGGACCTGAA GGTCACCCTC 
TACGACTATG ACCTCCTCTC CAAGGATGAA AAGATTGGTG AGACGGTCAT CGACCTGGAG 
AACAGGCTGC TGTCCAAGTT TGCGGCTCGC TGTGGACTCC CACAGACCTA CTGTGTCTCT 
GGACCGAACC AGTGGCGGGA CCAGCTCCAC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCGCAGAG TCAAGGCGCC TGTGTACCGG ACAGACCGTG TGATGTTTCA GGATAAAGAA 
TACTCCATTG AAGAAATAGA GGCTGGCAGG ATCCCAAACC CACACCTGGG CCCCATGGAG 
GAGCGTCTGG CTCTGCATGT GCTTCGGCAG CAGGGCCTGG TCCCAGAGCA CGTGGAGTCA 
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATTGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTGTTTC CGAAGGCCCT GGGGCGGCCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTGTATTATC TGGAATACCA GAGATGTGAT CCTGGATGAC 
CTGAGCCTCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GATTGGCTTT 
GAAGAACACA AGCAAAAGAC AGACGTGCAT TATCGCTCCC TGGGAGGTGA AGGCAACTTC 
AACTGGAGGT TCGTTTTCCC CTTCGACTAC CTGCCGGCTG AGCAAGTCTG TACGGTTGCC 
AAGAAGGATG CCTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACGAGTGATA 
TTCCAGATCT GGGACAATGA CAAGTTCTCC TTTGATGATT TTCTGGGCTC CCTGCAGCTC 
GATCTCAACC GCATGCCCAA GCCAGCCAAG ACAGCCGAGA AGTGCTCCTT GGACCAGCTG 
GATGACACTT ACCACCCAGA ATGGTTTGTG TCCCTTTTTG AGCAGAAAAC AGTGAAGGGC 
TGGTGGCCCT GTGTAGCAGA AGAGGGTGAG AAGAAGATAC TGGCGGGCAA GCTGGAAATG 
ACCTTGGAGA TTGTAACAGA GAGTGAGCAT GAGGAGCGGC CCGCTGGCCA GGGCCGGGAT 
GAGCCCAACA TGAACCCTAA GCTTGAGGAC CCAAGGCGCC CTGACACCTC CTTCCTGTGG 
TTCACCTCCC CATACAAGAC CATGAAGTTC ATCCTGTGGC GGCGTTTCCG GTGGGCCATC 
ATCCTCTTCA TCATCCTCTT CATCCTGCTG CTGTTCCTGG CCATCTTCAT CTACGCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361933.2 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360235.2 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X2, mRNA.

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CloneID	ORh253190
Clone ID Related Accession (Same CDS sequence)	XM_010361933.2
Accession Version	XM_010361933.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 25, 2019 this sequence version replaced XM_010361933.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Rhinopithecus roxellana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGTGGAGGC CCAGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCTGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCGGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA AACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTCC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCACCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGAGGAGAC TGCAGGTGCC GTCAAGTCTT CGAAAGCCGC AGACCTGGAT 
GACCACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGTTTCCT GGACCCCTAC GCAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGACT TCTGCTTTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAG 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCGGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TCGAGGTCAG CATTGGGAAC TATGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTATAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAATGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCACTGGTG 
GCTCAGCTGA TGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ATACCCTCTG CCACCCACCT GGACGAGTAC CTGTACCGGC TGCGCACCCG TCACCTGAGC 
CAAATCACGG AGGCTGCCCT GACCCTGAAG CTTGGCCACA GTGAGCTCCC TGCTGCTCTG 
GATCAGGCTG AGGACTGGCT CCTGCGTCTG CGTGCCCTTG CTGAGGAGCC CCAGAACAGC 
CTGCCGGATA TCATCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGTTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACCAGAC CAAGTTGGCC 
CTGGTCGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACCGGC 
AAGATCAAGC TACCCAAGGA GAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCAGAGAA GACCCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTT 
GTGGAGGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CTGATGTGAA TGGGGAGAAG GTGCTTCCCA AAGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATACCC CCGGACCGGA AGCCGAAGCA CTGGGTCCCT 
GTTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CATCTCGAGT ACCGCAAGAC AGACGCCTTC 
CGCCGCCGCC GCTGGCGCCG CCGCATGGAG CCCCTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTCG AGGGGGCACT GGGCGGCGTG GTGGACGACA AGAGTGAAGA TTCCATGTCC 
GCCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACAATTT CCTGCATATT TGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGCGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG CAGCCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTACGGCGC AGACGAGTTT ATGGGCCGCT GCATCTGTCA ACCGAGTTTG 
GAACGGATGC CCCGACTGGC CTGGTTCCCA CTGACCAGGG GCAGCCAGCC GATGGGGGAG 
CTGCTGGCCT CTTTTGAGCT TATCCAGAGA GAGAAGCCAG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC GAGGATCCTG GATGAGTCTG AGGACACAGA CTTGCCCTAC 
CCACCACCCC AAAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATTGAGGT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAGCATCT CCTCCCCGAG CCTTGTGGTA GAGTGTGGGG GCCAAACGGT GCAGTCCTGT 
GTCATTAGGA ACCTCCGGAA GAATCCCAAC TTTGACATCT GTACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCA TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCGTCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC CCTACTAACA CGGCTTCTCC TCCATCCAGT 
CCTCATGAGG AAGAGTTTAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTCA TTGGCGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCA CGCAGGGACC GCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGTTGGAGCC TGTGTTTGGA 
AAGATGTTTG AGCTGACCTG CACTCTGCCT CTAGAGAAGG ACCTGAAGGT CACCCTCTAC 
GACTATGACC TCCTCTCCAA GGATGAAAAG ATTGGTGAGA CGGTCATCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCACCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CGCAGAGTCA AGGCGCCTGT GTACCGGACA GACCGTGTGA TGTTTCAGGA TAAAGAATAC 
TCCATTGAAG AAATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC CATGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCGGCAGCAG GGCCTGGTCC CAGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATT GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTGTTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGCTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCG TTTTCCCCTT CGACTACCTG CCGGCTGAGC AAGTCTGTAC GGTTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGATATTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GACACTTACC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAGATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAACAGAGAG TGAGCATGAG GAGCGGCCCG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCTG ACACCTCCTT CCTGTGGTTC 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360235.2
CDS	379..6777
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEEETAGAVKSSKAADLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLHPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361933.2
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361933.2

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGTGGAGGC CCAGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCTGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCGGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA AACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTCC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCACCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGAGGAGAC TGCAGGTGCC GTCAAGTCTT CGAAAGCCGC AGACCTGGAT 
GACCACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGTTTCCT GGACCCCTAC GCAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGACT TCTGCTTTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAG 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCGGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TCGAGGTCAG CATTGGGAAC TATGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTATAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAATGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCACTGGTG 
GCTCAGCTGA TGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ATACCCTCTG CCACCCACCT GGACGAGTAC CTGTACCGGC TGCGCACCCG TCACCTGAGC 
CAAATCACGG AGGCTGCCCT GACCCTGAAG CTTGGCCACA GTGAGCTCCC TGCTGCTCTG 
GATCAGGCTG AGGACTGGCT CCTGCGTCTG CGTGCCCTTG CTGAGGAGCC CCAGAACAGC 
CTGCCGGATA TCATCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGTTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACCAGAC CAAGTTGGCC 
CTGGTCGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACCGGC 
AAGATCAAGC TACCCAAGGA GAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCAGAGAA GACCCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTT 
GTGGAGGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CTGATGTGAA TGGGGAGAAG GTGCTTCCCA AAGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATACCC CCGGACCGGA AGCCGAAGCA CTGGGTCCCT 
GTTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CATCTCGAGT ACCGCAAGAC AGACGCCTTC 
CGCCGCCGCC GCTGGCGCCG CCGCATGGAG CCCCTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTCG AGGGGGCACT GGGCGGCGTG GTGGACGACA AGAGTGAAGA TTCCATGTCC 
GCCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACAATTT CCTGCATATT TGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGCGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG CAGCCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTACGGCGC AGACGAGTTT ATGGGCCGCT GCATCTGTCA ACCGAGTTTG 
GAACGGATGC CCCGACTGGC CTGGTTCCCA CTGACCAGGG GCAGCCAGCC GATGGGGGAG 
CTGCTGGCCT CTTTTGAGCT TATCCAGAGA GAGAAGCCAG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC GAGGATCCTG GATGAGTCTG AGGACACAGA CTTGCCCTAC 
CCACCACCCC AAAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATTGAGGT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAGCATCT CCTCCCCGAG CCTTGTGGTA GAGTGTGGGG GCCAAACGGT GCAGTCCTGT 
GTCATTAGGA ACCTCCGGAA GAATCCCAAC TTTGACATCT GTACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCA TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCGTCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC CCTACTAACA CGGCTTCTCC TCCATCCAGT 
CCTCATGAGG AAGAGTTTAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTCA TTGGCGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCA CGCAGGGACC GCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGTTGGAGCC TGTGTTTGGA 
AAGATGTTTG AGCTGACCTG CACTCTGCCT CTAGAGAAGG ACCTGAAGGT CACCCTCTAC 
GACTATGACC TCCTCTCCAA GGATGAAAAG ATTGGTGAGA CGGTCATCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCACCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CGCAGAGTCA AGGCGCCTGT GTACCGGACA GACCGTGTGA TGTTTCAGGA TAAAGAATAC 
TCCATTGAAG AAATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC CATGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCGGCAGCAG GGCCTGGTCC CAGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATT GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTGTTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGCTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCG TTTTCCCCTT CGACTACCTG CCGGCTGAGC AAGTCTGTAC GGTTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGATATTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GACACTTACC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAGATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAACAGAGAG TGAGCATGAG GAGCGGCCCG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCTG ACACCTCCTT CCTGTGGTTC 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361935.2 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360237.2 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X3, mRNA.

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CloneID	ORh253191
Clone ID Related Accession (Same CDS sequence)	XM_010361935.2
Accession Version	XM_010361935.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 25, 2019 this sequence version replaced XM_010361935.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Rhinopithecus roxellana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGTGGA GGCCCAGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCT GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCG 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAAACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TCCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCACC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGTTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGAC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTCGAGGTCA GCATTGGGAA CTATGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGTAATGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGCTG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGACCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTT 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACCGG CAAGATCAAG CTACCCAAGG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCAGAGA AGACCCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACTGATGTGA ATGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATACC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGTTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCATCTCGAG 
TACCGCAAGA CAGACGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCAC TGGGCGGCGT GGTGGACGAC 
AAGAGTGAAG ATTCCATGTC CGCCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGCGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTCGGC GAGCCGGCCA CAGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTACGGCG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AACCGAGTTT GGAACGGATG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGATGGGGGA GCTGCTGGCC TCTTTTGAGC TTATCCAGAG AGAGAAGCCA 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGTCT 
GAGGACACAG ACTTGCCCTA CCCACCACCC CAAAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATTGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGGT AGAGTGTGGG 
GGCCAAACGG TGCAGTCCTG TGTCATTAGG AACCTCCGGA AGAATCCCAA CTTTGACATC 
TGTACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCGTC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCTACTAAC 
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTTA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACT TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTC 
ATTGGCGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC ACGCAGGGAC CGCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGTTGGAGC CTGTGTTTGG AAAGATGTTT GAGCTGACCT GCACTCTGCC TCTAGAGAAG 
GACCTGAAGG TCACCCTCTA CGACTATGAC CTCCTCTCCA AGGATGAAAA GATTGGTGAG 
ACGGTCATCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG CGGCTCGCTG TGGACTCCCA 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCACCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCGCAGAGTC AAGGCGCCTG TGTACCGGAC AGACCGTGTG 
ATGTTTCAGG ATAAAGAATA CTCCATTGAA GAAATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CCATGGAGGA GCGTCTGGCT CTGCATGTGC TTCGGCAGCA GGGCCTGGTC 
CCAGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT TGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTGTTTCCG AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAATACCAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGCTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC GTTTTCCCCT TCGACTACCT GCCGGCTGAG 
CAAGTCTGTA CGGTTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGATATT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGACACTTAC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT GTAGCAGAAG AGGGTGAGAA GAAGATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAACAGAGA GTGAGCATGA GGAGCGGCCC 
GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCT 
GACACCTCCT TCCTGTGGTT CACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360237.2
CDS	139..6498
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLHPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361935.2
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361935.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGTGGA GGCCCAGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCT GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCG 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAAACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TCCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCACC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGTTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGAC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTCGAGGTCA GCATTGGGAA CTATGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGTAATGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGCTG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGACCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTT 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACCGG CAAGATCAAG CTACCCAAGG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCAGAGA AGACCCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACTGATGTGA ATGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATACC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGTTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCATCTCGAG 
TACCGCAAGA CAGACGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCAC TGGGCGGCGT GGTGGACGAC 
AAGAGTGAAG ATTCCATGTC CGCCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGCGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTCGGC GAGCCGGCCA CAGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTACGGCG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AACCGAGTTT GGAACGGATG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGATGGGGGA GCTGCTGGCC TCTTTTGAGC TTATCCAGAG AGAGAAGCCA 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGTCT 
GAGGACACAG ACTTGCCCTA CCCACCACCC CAAAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATTGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGGT AGAGTGTGGG 
GGCCAAACGG TGCAGTCCTG TGTCATTAGG AACCTCCGGA AGAATCCCAA CTTTGACATC 
TGTACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCGTC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCTACTAAC 
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTTA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACT TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTC 
ATTGGCGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC ACGCAGGGAC CGCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGTTGGAGC CTGTGTTTGG AAAGATGTTT GAGCTGACCT GCACTCTGCC TCTAGAGAAG 
GACCTGAAGG TCACCCTCTA CGACTATGAC CTCCTCTCCA AGGATGAAAA GATTGGTGAG 
ACGGTCATCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG CGGCTCGCTG TGGACTCCCA 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCACCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCGCAGAGTC AAGGCGCCTG TGTACCGGAC AGACCGTGTG 
ATGTTTCAGG ATAAAGAATA CTCCATTGAA GAAATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CCATGGAGGA GCGTCTGGCT CTGCATGTGC TTCGGCAGCA GGGCCTGGTC 
CCAGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT TGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTGTTTCCG AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAATACCAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGCTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC GTTTTCCCCT TCGACTACCT GCCGGCTGAG 
CAAGTCTGTA CGGTTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGATATT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGACACTTAC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT GTAGCAGAAG AGGGTGAGAA GAAGATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAACAGAGA GTGAGCATGA GGAGCGGCCC 
GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCT 
GACACCTCCT TCCTGTGGTT CACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361936.2 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360238.2 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X4, mRNA.

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CloneID	ORh253192
Clone ID Related Accession (Same CDS sequence)	XM_010361936.2
Accession Version	XM_010361936.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 25, 2019 this sequence version replaced XM_010361936.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Rhinopithecus roxellana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGTGGAGGC CCAGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCTGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCGGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA AACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTCC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCACCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGTTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGACTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTC GAGGTCAGCA TTGGGAACTA TGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AATGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGCTGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGA CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTTGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACCGGCAA GATCAAGCTA CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCAGAGAAGA CCCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACT GATGTGAATG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATACCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGT TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA TCTCGAGTAC 
CGCAAGACAG ACGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCACTGG GCGGCGTGGT GGACGACAAG 
AGTGAAGATT CCATGTCCGC CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGCGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTCGGCGAG CCGGCCACAG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TACGGCGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAAC CGAGTTTGGA ACGGATGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA TGGGGGAGCT GCTGGCCTCT TTTGAGCTTA TCCAGAGAGA GAAGCCAGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACT TGCCCTACCC ACCACCCCAA AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATTGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC 
CAAACGGTGC AGTCCTGTGT CATTAGGAAC CTCCGGAAGA ATCCCAACTT TGACATCTGT 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCGTCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC TACTAACACG 
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCACG CAGGGACCGC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT AGAGAAGGAC 
CTGAAGGTCA CCCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCACCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCA TGGAGGAGCG TCTGGCTCTG CATGTGCTTC GGCAGCAGGG CCTGGTCCCA 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCGTT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACGG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTACCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCTGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360238.2
CDS	439..6795
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLHPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361936.2
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361936.2

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGTGGAGGC CCAGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCTGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCGGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA AACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTCC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCACCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGTTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGACTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTC GAGGTCAGCA TTGGGAACTA TGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AATGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGCTGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGA CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTTGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACCGGCAA GATCAAGCTA CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCAGAGAAGA CCCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACT GATGTGAATG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATACCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGT TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA TCTCGAGTAC 
CGCAAGACAG ACGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCACTGG GCGGCGTGGT GGACGACAAG 
AGTGAAGATT CCATGTCCGC CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGCGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTCGGCGAG CCGGCCACAG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TACGGCGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAAC CGAGTTTGGA ACGGATGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA TGGGGGAGCT GCTGGCCTCT TTTGAGCTTA TCCAGAGAGA GAAGCCAGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACT TGCCCTACCC ACCACCCCAA AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATTGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC 
CAAACGGTGC AGTCCTGTGT CATTAGGAAC CTCCGGAAGA ATCCCAACTT TGACATCTGT 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCGTCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC TACTAACACG 
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCACG CAGGGACCGC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT AGAGAAGGAC 
CTGAAGGTCA CCCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCACCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCA TGGAGGAGCG TCTGGCTCTG CATGTGCTTC GGCAGCAGGG CCTGGTCCCA 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCGTT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACGG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTACCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCTGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361937.2 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360239.2 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X5, mRNA.

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CloneID	ORh253193
Clone ID Related Accession (Same CDS sequence)	XM_010361937.2
Accession Version	XM_010361937.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 25, 2019 this sequence version replaced XM_010361937.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Rhinopithecus roxellana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGTGGA GGCCCAGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCT GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCG 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAAACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TCCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCACC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGAGGA GACTGCAGGT GCCGTCAAGT CTTCGAAAGC CGCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGTTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG ACTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTCGAGGT CAGCATTGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGTAATGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGC TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGACCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TTGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA GGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCAGA GAAGACCCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACTGATGT GAATGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATA CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGTTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCATCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC ACTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGCCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGCGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAACCGAGT 
TTGGAACGGA TGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGATGGGG 
GAGCTGCTGG CCTCTTTTGA GCTTATCCAG AGAGAGAAGC CAGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGT CTGAGGACAC AGACTTGCCC 
TACCCACCAC CCCAAAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GGTCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAGCA TCTCCTCCCC GAGCCTTGTG GTAGAGTGTG GGGGCCAAAC GGTGCAGTCC 
TGTGTCATTA GGAACCTCCG GAAGAATCCC AACTTTGACA TCTGTACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCATCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCG TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGGAGG AAGAGTTTAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTCA TTGGCGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCA CGCAGGGACC GCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGTTGGAGCC TGTGTTTGGA 
AAGATGTTTG AGCTGACCTG CACTCTGCCT CTAGAGAAGG ACCTGAAGGT CACCCTCTAC 
GACTATGACC TCCTCTCCAA GGATGAAAAG ATTGGTGAGA CGGTCATCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCACCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CGCAGAGTCA AGGCGCCTGT GTACCGGACA GACCGTGTGA TGTTTCAGGA TAAAGAATAC 
TCCATTGAAG AAATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC CATGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCGGCAGCAG GGCCTGGTCC CAGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATT GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTGTTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGCTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCG TTTTCCCCTT CGACTACCTG CCGGCTGAGC AAGTCTGTAC GGTTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGATATTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GACACTTACC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAGATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAACAGAGAG TGAGCATGAG GAGCGGCCCG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCTG ACACCTCCTT CCTGTGGTTC 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360239.2
CDS	139..6477
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEEETAGAVKSSKAADLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLHPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361937.2
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361937.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGTGGA GGCCCAGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCT GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCG 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAAACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TCCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCACC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGAGGA GACTGCAGGT GCCGTCAAGT CTTCGAAAGC CGCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGTTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG ACTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTCGAGGT CAGCATTGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGTAATGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGC TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGACCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TTGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA GGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCAGA GAAGACCCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACTGATGT GAATGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATA CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGTTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCATCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC ACTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGCCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGCGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAACCGAGT 
TTGGAACGGA TGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGATGGGG 
GAGCTGCTGG CCTCTTTTGA GCTTATCCAG AGAGAGAAGC CAGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGT CTGAGGACAC AGACTTGCCC 
TACCCACCAC CCCAAAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GGTCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAGCA TCTCCTCCCC GAGCCTTGTG GTAGAGTGTG GGGGCCAAAC GGTGCAGTCC 
TGTGTCATTA GGAACCTCCG GAAGAATCCC AACTTTGACA TCTGTACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCATCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCG TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGGAGG AAGAGTTTAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTCA TTGGCGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCA CGCAGGGACC GCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGTTGGAGCC TGTGTTTGGA 
AAGATGTTTG AGCTGACCTG CACTCTGCCT CTAGAGAAGG ACCTGAAGGT CACCCTCTAC 
GACTATGACC TCCTCTCCAA GGATGAAAAG ATTGGTGAGA CGGTCATCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCACCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CGCAGAGTCA AGGCGCCTGT GTACCGGACA GACCGTGTGA TGTTTCAGGA TAAAGAATAC 
TCCATTGAAG AAATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC CATGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCGGCAGCAG GGCCTGGTCC CAGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATT GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTGTTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGCTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCG TTTTCCCCTT CGACTACCTG CCGGCTGAGC AAGTCTGTAC GGTTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGATATTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GACACTTACC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAGATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAACAGAGAG TGAGCATGAG GAGCGGCCCG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCTG ACACCTCCTT CCTGTGGTTC 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361938.2 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360240.2 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X6, mRNA.

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CloneID	ORh253194
Clone ID Related Accession (Same CDS sequence)	XM_010361938.2
Accession Version	XM_010361938.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 25, 2019 this sequence version replaced XM_010361938.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Rhinopithecus roxellana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGTGGAGGC CCAGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCTGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCGGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA AACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTCC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCACCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGAGGAGAC TGCAGGTGCC GTCAAGTCTT CGAAAGCCGC AGACCTGGAT 
GACCACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGTTTCCT GGACCCCTAC GCAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGACT TCTGCTTTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAG 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCGGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TCGAGGTCAG CATTGGGAAC TATGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTATAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAATGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCACTGGTG 
GCTCAGCTGA TGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ATACCCTCTG CCACCCACCT GGACGAGTAC CTGTACCGGC TGCGCACCCG TCACCTGAGC 
CAAATCACGG AGGCTGCCCT GACCCTGAAG CTTGGCCACA GTGAGCTCCC TGCTGCTCTG 
GATCAGGCTG AGGACTGGCT CCTGCGTCTG CGTGCCCTTG CTGAGGAGCC CCAGAACAGC 
CTGCCGGATA TCATCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGTTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACCAGAC CAAGTTGGCC 
CTGGTCGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACCGGC 
AAGATCAAGC TACCCAAGGA GAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCAGAGAA GACCCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTT 
GTGGAGGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CTGATGTGAA TGGGGAGAAG GTGCTTCCCA AAGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATACCC CCGGACCGGA AGCCGAAGCA CTGGGTCCCT 
GTTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CATCTCGAGT ACCGCAAGAC AGACGCCTTC 
CGCCGCCGCC GCTGGCGCCG CCGCATGGAG CCCCTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTCG AGGGGGCACT GGGCGGCGTG GTGGACGACA AGAGTGAAGA TTCCATGTCC 
GCCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACAATTT CCTGCATATT TGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGCGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG CAGCCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTACGGCGC AGACGAGTTT ATGGGCCGCT GCATCTGTCA ACCGAGTTTG 
GAACGGATGC CCCGACTGGC CTGGTTCCCA CTGACCAGGG GCAGCCAGCC GATGGGGGAG 
CTGCTGGCCT CTTTTGAGCT TATCCAGAGA GAGAAGCCAG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC GAGGATCCTG GATGAGTCTG AGGACACAGA CTTGCCCTAC 
CCACCACCCC AAAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATTGAGGT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAGCATCT CCTCCCCGAG CCTTGTGGTA GAGTGTGGGG GCCAAACGGT GCAGTCCTGT 
GTCATTAGGA ACCTCCGGAA GAATCCCAAC TTTGACATCT GTACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCA TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCGTCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGGAGGAAG AGTTTATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACTTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTCATTG GCGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCCACGC AGGGACCGCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGT TGGAGCCTGT GTTTGGAAAG 
ATGTTTGAGC TGACCTGCAC TCTGCCTCTA GAGAAGGACC TGAAGGTCAC CCTCTACGAC 
TATGACCTCC TCTCCAAGGA TGAAAAGATT GGTGAGACGG TCATCGACCT GGAGAACAGG 
CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA CTCCCACAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCACCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC 
AGAGTCAAGG CGCCTGTGTA CCGGACAGAC CGTGTGATGT TTCAGGATAA AGAATACTCC 
ATTGAAGAAA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCCAT GGAGGAGCGT 
CTGGCTCTGC ATGTGCTTCG GCAGCAGGGC CTGGTCCCAG AGCACGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATTGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTG 
TTTCCGAAGG CCCTGGGGCG GCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT TATCTGGAAT ACCAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGC TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCGTTT TCCCCTTCGA CTACCTGCCG GCTGAGCAAG TCTGTACGGT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GATATTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC GAGAAGTGCT CCTTGGACCA GCTGGATGAC 
ACTTACCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCCTGTGTAG CAGAAGAGGG TGAGAAGAAG ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAA CAGAGAGTGA GCATGAGGAG CGGCCCGCTG GCCAGGGCCG GGATGAGCCC 
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCTGACA CCTCCTTCCT GTGGTTCACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTCATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360240.2
CDS	439..6774
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEEETAGAVKSSKAADLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLHPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361938.2
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361938.2

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGTGGAGGC CCAGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCTGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCGGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA AACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTCC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCACCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGAGGAGAC TGCAGGTGCC GTCAAGTCTT CGAAAGCCGC AGACCTGGAT 
GACCACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGTTTCCT GGACCCCTAC GCAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGACT TCTGCTTTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAG 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCGGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TCGAGGTCAG CATTGGGAAC TATGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTATAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAATGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCACTGGTG 
GCTCAGCTGA TGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ATACCCTCTG CCACCCACCT GGACGAGTAC CTGTACCGGC TGCGCACCCG TCACCTGAGC 
CAAATCACGG AGGCTGCCCT GACCCTGAAG CTTGGCCACA GTGAGCTCCC TGCTGCTCTG 
GATCAGGCTG AGGACTGGCT CCTGCGTCTG CGTGCCCTTG CTGAGGAGCC CCAGAACAGC 
CTGCCGGATA TCATCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGTTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACCAGAC CAAGTTGGCC 
CTGGTCGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACCGGC 
AAGATCAAGC TACCCAAGGA GAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCAGAGAA GACCCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTT 
GTGGAGGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CTGATGTGAA TGGGGAGAAG GTGCTTCCCA AAGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATACCC CCGGACCGGA AGCCGAAGCA CTGGGTCCCT 
GTTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CATCTCGAGT ACCGCAAGAC AGACGCCTTC 
CGCCGCCGCC GCTGGCGCCG CCGCATGGAG CCCCTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTCG AGGGGGCACT GGGCGGCGTG GTGGACGACA AGAGTGAAGA TTCCATGTCC 
GCCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACAATTT CCTGCATATT TGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGCGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG CAGCCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTACGGCGC AGACGAGTTT ATGGGCCGCT GCATCTGTCA ACCGAGTTTG 
GAACGGATGC CCCGACTGGC CTGGTTCCCA CTGACCAGGG GCAGCCAGCC GATGGGGGAG 
CTGCTGGCCT CTTTTGAGCT TATCCAGAGA GAGAAGCCAG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC GAGGATCCTG GATGAGTCTG AGGACACAGA CTTGCCCTAC 
CCACCACCCC AAAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATTGAGGT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAGCATCT CCTCCCCGAG CCTTGTGGTA GAGTGTGGGG GCCAAACGGT GCAGTCCTGT 
GTCATTAGGA ACCTCCGGAA GAATCCCAAC TTTGACATCT GTACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCA TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCGTCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGGAGGAAG AGTTTATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACTTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTCATTG GCGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCCACGC AGGGACCGCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGT TGGAGCCTGT GTTTGGAAAG 
ATGTTTGAGC TGACCTGCAC TCTGCCTCTA GAGAAGGACC TGAAGGTCAC CCTCTACGAC 
TATGACCTCC TCTCCAAGGA TGAAAAGATT GGTGAGACGG TCATCGACCT GGAGAACAGG 
CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA CTCCCACAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCACCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC 
AGAGTCAAGG CGCCTGTGTA CCGGACAGAC CGTGTGATGT TTCAGGATAA AGAATACTCC 
ATTGAAGAAA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCCAT GGAGGAGCGT 
CTGGCTCTGC ATGTGCTTCG GCAGCAGGGC CTGGTCCCAG AGCACGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATTGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTG 
TTTCCGAAGG CCCTGGGGCG GCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT TATCTGGAAT ACCAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGC TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCGTTT TCCCCTTCGA CTACCTGCCG GCTGAGCAAG TCTGTACGGT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GATATTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC GAGAAGTGCT CCTTGGACCA GCTGGATGAC 
ACTTACCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCCTGTGTAG CAGAAGAGGG TGAGAAGAAG ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAA CAGAGAGTGA GCATGAGGAG CGGCCCGCTG GCCAGGGCCG GGATGAGCCC 
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCTGACA CCTCCTTCCT GTGGTTCACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTCATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361939.2 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360241.2 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X7, mRNA.

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CloneID	ORh253195
Clone ID Related Accession (Same CDS sequence)	XM_010361939.2
Accession Version	XM_010361939.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 25, 2019 this sequence version replaced XM_010361939.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Rhinopithecus roxellana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGTGGAGGC 
CCAGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCTGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCGGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA AACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTCC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCACCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGAC TGCAGGTGCC 
GTCAAGTCTT CGAAAGCCGC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGTTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGACT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TCGAGGTCAG CATTGGGAAC 
TATGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GTAATGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGCTGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GACCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTTG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACCGGC AAGATCAAGC TACCCAAGGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCAGAGAA GACCCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CTGATGTGAA TGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATACCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GTTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CATCTCGAGT ACCGCAAGAC AGACGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCACT GGGCGGCGTG 
GTGGACGACA AGAGTGAAGA TTCCATGTCC GCCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGCGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTACGGCGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA ACCGAGTTTG GAACGGATGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GATGGGGGAG CTGCTGGCCT CTTTTGAGCT TATCCAGAGA 
GAGAAGCCAG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGTCTG AGGACACAGA CTTGCCCTAC CCACCACCCC AAAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATTGAGGT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAGCATCT CCTCCCCGAG CCTTGTGGTA 
GAGTGTGGGG GCCAAACGGT GCAGTCCTGT GTCATTAGGA ACCTCCGGAA GAATCCCAAC 
TTTGACATCT GTACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCA TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCGTCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC 
CCTACTAACA CGGCTTCTCC TCCATCCAGT CCTCATGAGG AAGAGTTTAT CGATTGGTGG 
AGCAAATTCT TTGCCTCCAT AGGGGAGAGG GAAAAGTGCG GCTCCTACTT GGAGAAGGAT 
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTGTAC CGGGGCAAGA CGCAGGAGGA GACAGAAGAT 
CCATCTGTCA TTGGCGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCA 
GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTGGCCA CGCAGGGACC GCAGGAGTGC 
TTGGTCCGTA TCTACATTGT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCCTGCA CGTTGGAGCC TGTGTTTGGA AAGATGTTTG AGCTGACCTG CACTCTGCCT 
CTAGAGAAGG ACCTGAAGGT CACCCTCTAC GACTATGACC TCCTCTCCAA GGATGAAAAG 
ATTGGTGAGA CGGTCATCGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT 
GGACTCCCAC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGCGGGACCA GCTCCACCCC 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CGCAGAGTCA AGGCGCCTGT GTACCGGACA 
GACCGTGTGA TGTTTCAGGA TAAAGAATAC TCCATTGAAG AAATAGAGGC TGGCAGGATC 
CCAAACCCAC ACCTGGGCCC CATGGAGGAG CGTCTGGCTC TGCATGTGCT TCGGCAGCAG 
GGCCTGGTCC CAGAGCACGT GGAGTCACGG CCCCTCTACA GCCCCCTGCA GCCAGACATT 
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTGTTTCCGA AGGCCCTGGG GCGGCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATTATCTGG 
AATACCAGAG ATGTGATCCT GGATGACCTG AGCCTCACGG GGGAGAAGAT GAGCGACATT 
TATGTGAAAG GTTGGATGAT TGGCTTTGAA GAACACAAGC AAAAGACAGA CGTGCATTAT 
CGCTCCCTGG GAGGTGAAGG CAACTTCAAC TGGAGGTTCG TTTTCCCCTT CGACTACCTG 
CCGGCTGAGC AAGTCTGTAC GGTTGCCAAG AAGGATGCCT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACG AGTGATATTC CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GATGATTTTC TGGGCTCCCT GCAGCTCGAT CTCAACCGCA TGCCCAAGCC AGCCAAGACA 
GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTACC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAAACAGT GAAGGGCTGG TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG 
AAGATACTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TAACAGAGAG TGAGCATGAG 
GAGCGGCCCG CTGGCCAGGG CCGGGATGAG CCCAACATGA ACCCTAAGCT TGAGGACCCA 
AGGCGCCCTG ACACCTCCTT CCTGTGGTTC ACCTCCCCAT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GTTTCCGGTG GGCCATCATC CTCTTCATCA TCCTCTTCAT CCTGCTGCTG 
TTCCTGGCCA TCTTCATCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360241.2
CDS	139..6447
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEEETAGAVKSSKAADLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLHPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361939.2
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361939.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGTGGAGGC 
CCAGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCTGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCGGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA AACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTCC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCACCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGAC TGCAGGTGCC 
GTCAAGTCTT CGAAAGCCGC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGTTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGACT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TCGAGGTCAG CATTGGGAAC 
TATGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GTAATGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGCTGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GACCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTTG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACCGGC AAGATCAAGC TACCCAAGGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCAGAGAA GACCCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CTGATGTGAA TGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATACCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GTTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CATCTCGAGT ACCGCAAGAC AGACGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCACT GGGCGGCGTG 
GTGGACGACA AGAGTGAAGA TTCCATGTCC GCCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGCGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTACGGCGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA ACCGAGTTTG GAACGGATGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GATGGGGGAG CTGCTGGCCT CTTTTGAGCT TATCCAGAGA 
GAGAAGCCAG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGTCTG AGGACACAGA CTTGCCCTAC CCACCACCCC AAAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATTGAGGT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAGCATCT CCTCCCCGAG CCTTGTGGTA 
GAGTGTGGGG GCCAAACGGT GCAGTCCTGT GTCATTAGGA ACCTCCGGAA GAATCCCAAC 
TTTGACATCT GTACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCA TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCGTCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC 
CCTACTAACA CGGCTTCTCC TCCATCCAGT CCTCATGAGG AAGAGTTTAT CGATTGGTGG 
AGCAAATTCT TTGCCTCCAT AGGGGAGAGG GAAAAGTGCG GCTCCTACTT GGAGAAGGAT 
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTGTAC CGGGGCAAGA CGCAGGAGGA GACAGAAGAT 
CCATCTGTCA TTGGCGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCA 
GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTGGCCA CGCAGGGACC GCAGGAGTGC 
TTGGTCCGTA TCTACATTGT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCCTGCA CGTTGGAGCC TGTGTTTGGA AAGATGTTTG AGCTGACCTG CACTCTGCCT 
CTAGAGAAGG ACCTGAAGGT CACCCTCTAC GACTATGACC TCCTCTCCAA GGATGAAAAG 
ATTGGTGAGA CGGTCATCGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT 
GGACTCCCAC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGCGGGACCA GCTCCACCCC 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CGCAGAGTCA AGGCGCCTGT GTACCGGACA 
GACCGTGTGA TGTTTCAGGA TAAAGAATAC TCCATTGAAG AAATAGAGGC TGGCAGGATC 
CCAAACCCAC ACCTGGGCCC CATGGAGGAG CGTCTGGCTC TGCATGTGCT TCGGCAGCAG 
GGCCTGGTCC CAGAGCACGT GGAGTCACGG CCCCTCTACA GCCCCCTGCA GCCAGACATT 
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTGTTTCCGA AGGCCCTGGG GCGGCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATTATCTGG 
AATACCAGAG ATGTGATCCT GGATGACCTG AGCCTCACGG GGGAGAAGAT GAGCGACATT 
TATGTGAAAG GTTGGATGAT TGGCTTTGAA GAACACAAGC AAAAGACAGA CGTGCATTAT 
CGCTCCCTGG GAGGTGAAGG CAACTTCAAC TGGAGGTTCG TTTTCCCCTT CGACTACCTG 
CCGGCTGAGC AAGTCTGTAC GGTTGCCAAG AAGGATGCCT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACG AGTGATATTC CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GATGATTTTC TGGGCTCCCT GCAGCTCGAT CTCAACCGCA TGCCCAAGCC AGCCAAGACA 
GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTACC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAAACAGT GAAGGGCTGG TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG 
AAGATACTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TAACAGAGAG TGAGCATGAG 
GAGCGGCCCG CTGGCCAGGG CCGGGATGAG CCCAACATGA ACCCTAAGCT TGAGGACCCA 
AGGCGCCCTG ACACCTCCTT CCTGTGGTTC ACCTCCCCAT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GTTTCCGGTG GGCCATCATC CTCTTCATCA TCCTCTTCAT CCTGCTGCTG 
TTCCTGGCCA TCTTCATCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361940.2 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360242.2 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X8, mRNA.

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CloneID	ORh253196
Clone ID Related Accession (Same CDS sequence)	XM_010361940.2
Accession Version	XM_010361940.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 25, 2019 this sequence version replaced XM_010361940.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Rhinopithecus roxellana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG TGGAGGCCCA 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCTGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCGGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAAAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTCCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CACCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGACTGC AGGTGCCGTC 
AAGTCTTCGA AAGCCGCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GTTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGACTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTCG AGGTCAGCAT TGGGAACTAT 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGTA ATGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGCTGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGAC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTTGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACCGGCAAG ATCAAGCTAC CCAAGGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CAGAGAAGAC CCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACTG ATGTGAATGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATACCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGTT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAT 
CTCGAGTACC GCAAGACAGA CGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCACTGGG CGGCGTGGTG 
GACGACAAGA GTGAAGATTC CATGTCCGCC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GCGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTCGGCGAGC CGGCCACAGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ACGGCGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAACC GAGTTTGGAA CGGATGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAT GGGGGAGCTG CTGGCCTCTT TTGAGCTTAT CCAGAGAGAG 
AAGCCAGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACTT GCCCTACCCA CCACCCCAAA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TTGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGGTAGAG 
TGTGGGGGCC AAACGGTGCA GTCCTGTGTC ATTAGGAACC TCCGGAAGAA TCCCAACTTT 
GACATCTGTA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCGTCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCT 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATGAGGAAG AGTTTATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GCGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCACGC AGGGACCGCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGT TGGAGCCTGT GTTTGGAAAG ATGTTTGAGC TGACCTGCAC TCTGCCTCTA 
GAGAAGGACC TGAAGGTCAC CCTCTACGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGTGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCACCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAGG CGCCTGTGTA CCGGACAGAC 
CGTGTGATGT TTCAGGATAA AGAATACTCC ATTGAAGAAA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCCAT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCG GCAGCAGGGC 
CTGGTCCCAG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCGTTT TCCCCTTCGA CTACCTGCCG 
GCTGAGCAAG TCTGTACGGT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GATATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTACCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCCGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCTGACA CCTCCTTCCT GTGGTTCACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360242.2
CDS	439..6744
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEEETAGAVKSSKAADLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLHPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361940.2
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361940.2

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG TGGAGGCCCA 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCTGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCGGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAAAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTCCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CACCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGACTGC AGGTGCCGTC 
AAGTCTTCGA AAGCCGCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GTTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGACTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTCG AGGTCAGCAT TGGGAACTAT 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGTA ATGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGCTGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGAC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTTGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACCGGCAAG ATCAAGCTAC CCAAGGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CAGAGAAGAC CCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACTG ATGTGAATGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATACCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGTT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAT 
CTCGAGTACC GCAAGACAGA CGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCACTGGG CGGCGTGGTG 
GACGACAAGA GTGAAGATTC CATGTCCGCC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GCGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTCGGCGAGC CGGCCACAGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ACGGCGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAACC GAGTTTGGAA CGGATGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAT GGGGGAGCTG CTGGCCTCTT TTGAGCTTAT CCAGAGAGAG 
AAGCCAGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACTT GCCCTACCCA CCACCCCAAA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TTGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGGTAGAG 
TGTGGGGGCC AAACGGTGCA GTCCTGTGTC ATTAGGAACC TCCGGAAGAA TCCCAACTTT 
GACATCTGTA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCGTCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCT 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATGAGGAAG AGTTTATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GCGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCACGC AGGGACCGCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGT TGGAGCCTGT GTTTGGAAAG ATGTTTGAGC TGACCTGCAC TCTGCCTCTA 
GAGAAGGACC TGAAGGTCAC CCTCTACGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGTGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCACCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAGG CGCCTGTGTA CCGGACAGAC 
CGTGTGATGT TTCAGGATAA AGAATACTCC ATTGAAGAAA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCCAT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCG GCAGCAGGGC 
CTGGTCCCAG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCGTTT TCCCCTTCGA CTACCTGCCG 
GCTGAGCAAG TCTGTACGGT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GATATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTACCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCCGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCTGACA CCTCCTTCCT GTGGTTCACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361941.2 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360243.2 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X9, mRNA.

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CloneID	ORh253197
Clone ID Related Accession (Same CDS sequence)	XM_010361941.2
Accession Version	XM_010361941.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 25, 2019 this sequence version replaced XM_010361941.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Rhinopithecus roxellana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGTGGA GGCCCAGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCT GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCG 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAAACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TCCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCACC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGTTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGAC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTCGAGGTCA GCATTGGGAA CTATGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGTAATGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGCTG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGACCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTT 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACCGG CAAGATCAAG CTACCCAAGG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCAGAGA AGACCCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACTGATGTGA ATGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATACC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGTTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCATCTCGAG 
TACCGCAAGA CAGACGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCAC TGGGCGGCGT GGTGGACGAC 
AAGAGTGAAG ATTCCATGTC CGCCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGCGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTCGGC GAGCCGGCCA CAGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTACGGCG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AACCGAGTTT GGAACGGATG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGATGGGGGA GCTGCTGGCC TCTTTTGAGC TTATCCAGAG AGAGAAGCCA 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGTCT 
GAGGACACAG ACTTGCCCTA CCCACCACCC CAAAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATTGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGGT AGAGTGTGGG 
GGCCAAACGG TGCAGTCCTG TGTCATTAGG AACCTCCGGA AGAATCCCAA CTTTGACATC 
TGTACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCGTC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCACG CAGGGACCGC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT AGAGAAGGAC 
CTGAAGGTCA CCCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCACCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCA TGGAGGAGCG TCTGGCTCTG CATGTGCTTC GGCAGCAGGG CCTGGTCCCA 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCGTT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACGG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTACCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCTGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360243.2
CDS	139..6435
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLHPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361941.2
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361941.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGTGGA GGCCCAGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCT GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCG 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAAACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TCCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCACC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGTTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGAC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTCGAGGTCA GCATTGGGAA CTATGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGTAATGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGCTG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGACCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTT 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACCGG CAAGATCAAG CTACCCAAGG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCAGAGA AGACCCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACTGATGTGA ATGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATACC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGTTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCATCTCGAG 
TACCGCAAGA CAGACGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCAC TGGGCGGCGT GGTGGACGAC 
AAGAGTGAAG ATTCCATGTC CGCCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGCGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTCGGC GAGCCGGCCA CAGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTACGGCG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AACCGAGTTT GGAACGGATG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGATGGGGGA GCTGCTGGCC TCTTTTGAGC TTATCCAGAG AGAGAAGCCA 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGTCT 
GAGGACACAG ACTTGCCCTA CCCACCACCC CAAAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATTGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGGT AGAGTGTGGG 
GGCCAAACGG TGCAGTCCTG TGTCATTAGG AACCTCCGGA AGAATCCCAA CTTTGACATC 
TGTACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCGTC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCACG CAGGGACCGC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT AGAGAAGGAC 
CTGAAGGTCA CCCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCACCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCA TGGAGGAGCG TCTGGCTCTG CATGTGCTTC GGCAGCAGGG CCTGGTCCCA 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCGTT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACGG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTACCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCTGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361942.2 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360244.2 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X10, mRNA.

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CloneID	ORh253198
Clone ID Related Accession (Same CDS sequence)	XM_010361942.2
Accession Version	XM_010361942.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 25, 2019 this sequence version replaced XM_010361942.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Rhinopithecus roxellana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGTGGAGGC CCAGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCTGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCGGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA AACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTCC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCACCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGTTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGACTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTC GAGGTCAGCA TTGGGAACTA TGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AATGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGCTGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGA CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTTGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACCGGCAA GATCAAGCTA CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCAGAGAAGA CCCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACT GATGTGAATG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATACCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGT TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA TCTCGAGTAC 
CGCAAGACAG ACGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCACTGG GCGGCGTGGT GGACGACAAG 
AGTGAAGATT CCATGTCCGC CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGCGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTCGGCGAG CCGGCCACAG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TACGGCGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAAC CGAGTTTGGA ACGGATGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA TGGGGGAGCT GCTGGCCTCT TTTGAGCTTA TCCAGAGAGA GAAGCCAGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACT TGCCCTACCC ACCACCCCAA AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATTGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC 
CAAACGGTGC AGTCCTGTGT CATTAGGAAC CTCCGGAAGA ATCCCAACTT TGACATCTGT 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCGTCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GGAGGAAGAG TTTATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTCATTGGC 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCACGCAG GGACCGCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGTTG 
GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG ACCTGCACTC TGCCTCTAGA GAAGGACCTG 
AAGGTCACCC TCTACGACTA TGACCTCCTC TCCAAGGATG AAAAGATTGG TGAGACGGTC 
ATCGACCTGG AGAACAGGCT GCTGTCCAAG TTTGCGGCTC GCTGTGGACT CCCACAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC ACCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG CCTGTGTACC GGACAGACCG TGTGATGTTT 
CAGGATAAAG AATACTCCAT TGAAGAAATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCCATGG AGGAGCGTCT GGCTCTGCAT GTGCTTCGGC AGCAGGGCCT GGTCCCAGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATTGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTGTT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGCTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCGTTTTC CCCTTCGACT ACCTGCCGGC TGAGCAAGTC 
TGTACGGTTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGA TATTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGACAC TTACCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAACA GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCTGACACC 
TCCTTCCTGT GGTTCACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360244.2
CDS	439..6732
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLHPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361942.2
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361942.2

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGTGGAGGC CCAGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCTGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCGGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA AACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTCC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCACCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGTTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGACTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTC GAGGTCAGCA TTGGGAACTA TGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AATGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGCTGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGA CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTTGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACCGGCAA GATCAAGCTA CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCAGAGAAGA CCCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACT GATGTGAATG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATACCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGT TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA TCTCGAGTAC 
CGCAAGACAG ACGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCACTGG GCGGCGTGGT GGACGACAAG 
AGTGAAGATT CCATGTCCGC CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGCGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTCGGCGAG CCGGCCACAG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TACGGCGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAAC CGAGTTTGGA ACGGATGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA TGGGGGAGCT GCTGGCCTCT TTTGAGCTTA TCCAGAGAGA GAAGCCAGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACT TGCCCTACCC ACCACCCCAA AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATTGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC 
CAAACGGTGC AGTCCTGTGT CATTAGGAAC CTCCGGAAGA ATCCCAACTT TGACATCTGT 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCGTCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GGAGGAAGAG TTTATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTCATTGGC 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCACGCAG GGACCGCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGTTG 
GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG ACCTGCACTC TGCCTCTAGA GAAGGACCTG 
AAGGTCACCC TCTACGACTA TGACCTCCTC TCCAAGGATG AAAAGATTGG TGAGACGGTC 
ATCGACCTGG AGAACAGGCT GCTGTCCAAG TTTGCGGCTC GCTGTGGACT CCCACAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC ACCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG CCTGTGTACC GGACAGACCG TGTGATGTTT 
CAGGATAAAG AATACTCCAT TGAAGAAATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCCATGG AGGAGCGTCT GGCTCTGCAT GTGCTTCGGC AGCAGGGCCT GGTCCCAGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATTGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTGTT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGCTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCGTTTTC CCCTTCGACT ACCTGCCGGC TGAGCAAGTC 
TGTACGGTTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGA TATTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGACAC TTACCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAACA GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCTGACACC 
TCCTTCCTGT GGTTCACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361943.2 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360245.2 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X11, mRNA.

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CloneID	ORh253199
Clone ID Related Accession (Same CDS sequence)	XM_010361943.2
Accession Version	XM_010361943.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 25, 2019 this sequence version replaced XM_010361943.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Rhinopithecus roxellana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGTGGAGGC 
CCAGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCTGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCGGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA AACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTCC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCACCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CCACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGTTTCCTGG ACCCCTACGC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGACTTC TGCTTTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC GGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTC 
GAGGTCAGCA TTGGGAACTA TGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ATAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AATGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CACTGGTGGC TCAGCTGATG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAT ACCCTCTGCC 
ACCCACCTGG ACGAGTACCT GTACCGGCTG CGCACCCGTC ACCTGAGCCA AATCACGGAG 
GCTGCCCTGA CCCTGAAGCT TGGCCACAGT GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTTGCT GAGGAGCCCC AGAACAGCCT GCCGGATATC 
ATCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GTTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACCAGACCA AGTTGGCCCT GGTCGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACCGGCAA GATCAAGCTA 
CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCAGAGAAGA CCCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTTGT GGAGGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACT 
GATGTGAATG GGGAGAAGGT GCTTCCCAAA GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATACCCCC GGACCGGAAG CCGAAGCACT GGGTCCCTGT TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAGATG 
GAAGCGCTGA AGAGGCATAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA TCTCGAGTAC CGCAAGACAG ACGCCTTCCG CCGCCGCCGC 
TGGCGCCGCC GCATGGAGCC CCTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG 
GGGGCACTGG GCGGCGTGGT GGACGACAAG AGTGAAGATT CCATGTCCGC CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACAATTTCC TGCATATTTG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGCGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTCGGCGAG 
CCGGCCACAG TTGCTGAGCA GCCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TACGGCGCAG ACGAGTTTAT GGGCCGCTGC ATCTGTCAAC CGAGTTTGGA ACGGATGCCC 
CGACTGGCCT GGTTCCCACT GACCAGGGGC AGCCAGCCGA TGGGGGAGCT GCTGGCCTCT 
TTTGAGCTTA TCCAGAGAGA GAAGCCAGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCGA GGATCCTGGA TGAGTCTGAG GACACAGACT TGCCCTACCC ACCACCCCAA 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATTGAGGTCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGCATCTCC 
TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC CAAACGGTGC AGTCCTGTGT CATTAGGAAC 
CTCCGGAAGA ATCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCATC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCGTCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC TACTAACACG GCTTCTCCTC CATCCAGTCC TCATGAGGAA 
GAGTTTATCG ATTGGTGGAG CAAATTCTTT GCCTCCATAG GGGAGAGGGA AAAGTGCGGC 
TCCTACTTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTGTACCG GGGCAAGACG 
CAGGAGGAGA CAGAAGATCC ATCTGTCATT GGCGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCAGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTGGCCACG 
CAGGGACCGC AGGAGTGCTT GGTCCGTATC TACATTGTCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCCTGCACG TTGGAGCCTG TGTTTGGAAA GATGTTTGAG 
CTGACCTGCA CTCTGCCTCT AGAGAAGGAC CTGAAGGTCA CCCTCTACGA CTATGACCTC 
CTCTCCAAGG ATGAAAAGAT TGGTGAGACG GTCATCGACC TGGAGAACAG GCTGCTGTCC 
AAGTTTGCGG CTCGCTGTGG ACTCCCACAG ACCTACTGTG TCTCTGGACC GAACCAGTGG 
CGGGACCAGC TCCACCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCG CAGAGTCAAG 
GCGCCTGTGT ACCGGACAGA CCGTGTGATG TTTCAGGATA AAGAATACTC CATTGAAGAA 
ATAGAGGCTG GCAGGATCCC AAACCCACAC CTGGGCCCCA TGGAGGAGCG TCTGGCTCTG 
CATGTGCTTC GGCAGCAGGG CCTGGTCCCA GAGCACGTGG AGTCACGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATTGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT GTTTCCGAAG 
GCCCTGGGGC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA TACCAGAGAT GTGATCCTGG ATGACCTGAG CCTCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGATTG GCTTTGAAGA ACACAAGCAA 
AAGACAGACG TGCATTATCG CTCCCTGGGA GGTGAAGGCA ACTTCAACTG GAGGTTCGTT 
TTCCCCTTCG ACTACCTGCC GGCTGAGCAA GTCTGTACGG TTGCCAAGAA GGATGCCTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACGAG TGATATTCCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA TGATTTTCTG GGCTCCCTGC AGCTCGATCT CAACCGCATG 
CCCAAGCCAG CCAAGACAGC CGAGAAGTGC TCCTTGGACC AGCTGGATGA CACTTACCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAAACAGTGA AGGGCTGGTG GCCCTGTGTA 
GCAGAAGAGG GTGAGAAGAA GATACTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTA 
ACAGAGAGTG AGCATGAGGA GCGGCCCGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCTGAC ACCTCCTTCC TGTGGTTCAC CTCCCCATAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGGG CCATCATCCT CTTCATCATC 
CTCTTCATCC TGCTGCTGTT CCTGGCCATC TTCATCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360245.2
CDS	139..6405
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLHPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361943.2
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361943.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGTGGAGGC 
CCAGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCTGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCGGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA AACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTCC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCACCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CCACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGTTTCCTGG ACCCCTACGC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGACTTC TGCTTTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC GGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTC 
GAGGTCAGCA TTGGGAACTA TGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ATAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AATGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CACTGGTGGC TCAGCTGATG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAT ACCCTCTGCC 
ACCCACCTGG ACGAGTACCT GTACCGGCTG CGCACCCGTC ACCTGAGCCA AATCACGGAG 
GCTGCCCTGA CCCTGAAGCT TGGCCACAGT GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTTGCT GAGGAGCCCC AGAACAGCCT GCCGGATATC 
ATCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GTTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACCAGACCA AGTTGGCCCT GGTCGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACCGGCAA GATCAAGCTA 
CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCAGAGAAGA CCCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTTGT GGAGGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACT 
GATGTGAATG GGGAGAAGGT GCTTCCCAAA GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATACCCCC GGACCGGAAG CCGAAGCACT GGGTCCCTGT TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAGATG 
GAAGCGCTGA AGAGGCATAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA TCTCGAGTAC CGCAAGACAG ACGCCTTCCG CCGCCGCCGC 
TGGCGCCGCC GCATGGAGCC CCTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG 
GGGGCACTGG GCGGCGTGGT GGACGACAAG AGTGAAGATT CCATGTCCGC CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACAATTTCC TGCATATTTG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGCGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTCGGCGAG 
CCGGCCACAG TTGCTGAGCA GCCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TACGGCGCAG ACGAGTTTAT GGGCCGCTGC ATCTGTCAAC CGAGTTTGGA ACGGATGCCC 
CGACTGGCCT GGTTCCCACT GACCAGGGGC AGCCAGCCGA TGGGGGAGCT GCTGGCCTCT 
TTTGAGCTTA TCCAGAGAGA GAAGCCAGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCGA GGATCCTGGA TGAGTCTGAG GACACAGACT TGCCCTACCC ACCACCCCAA 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATTGAGGTCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGCATCTCC 
TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC CAAACGGTGC AGTCCTGTGT CATTAGGAAC 
CTCCGGAAGA ATCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCATC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCGTCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC TACTAACACG GCTTCTCCTC CATCCAGTCC TCATGAGGAA 
GAGTTTATCG ATTGGTGGAG CAAATTCTTT GCCTCCATAG GGGAGAGGGA AAAGTGCGGC 
TCCTACTTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTGTACCG GGGCAAGACG 
CAGGAGGAGA CAGAAGATCC ATCTGTCATT GGCGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCAGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTGGCCACG 
CAGGGACCGC AGGAGTGCTT GGTCCGTATC TACATTGTCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCCTGCACG TTGGAGCCTG TGTTTGGAAA GATGTTTGAG 
CTGACCTGCA CTCTGCCTCT AGAGAAGGAC CTGAAGGTCA CCCTCTACGA CTATGACCTC 
CTCTCCAAGG ATGAAAAGAT TGGTGAGACG GTCATCGACC TGGAGAACAG GCTGCTGTCC 
AAGTTTGCGG CTCGCTGTGG ACTCCCACAG ACCTACTGTG TCTCTGGACC GAACCAGTGG 
CGGGACCAGC TCCACCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCG CAGAGTCAAG 
GCGCCTGTGT ACCGGACAGA CCGTGTGATG TTTCAGGATA AAGAATACTC CATTGAAGAA 
ATAGAGGCTG GCAGGATCCC AAACCCACAC CTGGGCCCCA TGGAGGAGCG TCTGGCTCTG 
CATGTGCTTC GGCAGCAGGG CCTGGTCCCA GAGCACGTGG AGTCACGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATTGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT GTTTCCGAAG 
GCCCTGGGGC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA TACCAGAGAT GTGATCCTGG ATGACCTGAG CCTCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGATTG GCTTTGAAGA ACACAAGCAA 
AAGACAGACG TGCATTATCG CTCCCTGGGA GGTGAAGGCA ACTTCAACTG GAGGTTCGTT 
TTCCCCTTCG ACTACCTGCC GGCTGAGCAA GTCTGTACGG TTGCCAAGAA GGATGCCTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACGAG TGATATTCCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA TGATTTTCTG GGCTCCCTGC AGCTCGATCT CAACCGCATG 
CCCAAGCCAG CCAAGACAGC CGAGAAGTGC TCCTTGGACC AGCTGGATGA CACTTACCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAAACAGTGA AGGGCTGGTG GCCCTGTGTA 
GCAGAAGAGG GTGAGAAGAA GATACTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTA 
ACAGAGAGTG AGCATGAGGA GCGGCCCGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCTGAC ACCTCCTTCC TGTGGTTCAC CTCCCCATAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGGG CCATCATCCT CTTCATCATC 
CTCTTCATCC TGCTGCTGTT CCTGGCCATC TTCATCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361944.2 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360246.2 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X12, mRNA.

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CloneID	ORh253200
Clone ID Related Accession (Same CDS sequence)	XM_010361944.2
Accession Version	XM_010361944.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 25, 2019 this sequence version replaced XM_010361944.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Rhinopithecus roxellana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG TGGAGGCCCA 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCTGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCGGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAAAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTCCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CACCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACCA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGT 
TTCCTGGACC CCTACGCAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGACTTCTGC TTTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAGCAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCGGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTCGAG 
GTCAGCATTG GGAACTATGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTATA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGTAAT 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCAC TGGTGGCTCA GCTGATGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGATACC CTCTGCCACC 
CACCTGGACG AGTACCTGTA CCGGCTGCGC ACCCGTCACC TGAGCCAAAT CACGGAGGCT 
GCCCTGACCC TGAAGCTTGG CCACAGTGAG CTCCCTGCTG CTCTGGATCA GGCTGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTTGCTGAG GAGCCCCAGA ACAGCCTGCC GGATATCATC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGTT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC CAGACCAAGT TGGCCCTGGT CGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CCGGCAAGAT CAAGCTACCC 
AAGGAGAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCA 
GAGAAGACCC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTTGTGGA GGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACTGAT 
GTGAATGGGG AGAAGGTGCT TCCCAAAGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TACCCCCGGA CCGGAAGCCG AAGCACTGGG TCCCTGTTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAGATGGAA 
GCGCTGAAGA GGCATAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCATCT CGAGTACCGC AAGACAGACG CCTTCCGCCG CCGCCGCTGG 
CGCCGCCGCA TGGAGCCCCT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTCGAGGGG 
GCACTGGGCG GCGTGGTGGA CGACAAGAGT GAAGATTCCA TGTCCGCCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC AATTTCCTGC ATATTTGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGC GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT CGGCGAGCCG 
GCCACAGTTG CTGAGCAGCC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAC 
GGCGCAGACG AGTTTATGGG CCGCTGCATC TGTCAACCGA GTTTGGAACG GATGCCCCGA 
CTGGCCTGGT TCCCACTGAC CAGGGGCAGC CAGCCGATGG GGGAGCTGCT GGCCTCTTTT 
GAGCTTATCC AGAGAGAGAA GCCAGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCGAGGA TCCTGGATGA GTCTGAGGAC ACAGACTTGC CCTACCCACC ACCCCAAAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGGTAGAGTG TGGGGGCCAA ACGGTGCAGT CCTGTGTCAT TAGGAACCTC 
CGGAAGAATC CCAACTTTGA CATCTGTACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CGTCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCTAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCACGCAG 
GGACCGCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTAGA GAAGGACCTG AAGGTCACCC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC ACCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCATGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCGGC AGCAGGGCCT GGTCCCAGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCGTTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACGGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTACCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCTGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360246.2
CDS	439..6702
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLHPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361944.2
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361944.2

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG TGGAGGCCCA 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCTGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCGGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAAAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTCCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CACCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACCA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGT 
TTCCTGGACC CCTACGCAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGACTTCTGC TTTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAGCAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCGGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTCGAG 
GTCAGCATTG GGAACTATGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTATA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGTAAT 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCAC TGGTGGCTCA GCTGATGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGATACC CTCTGCCACC 
CACCTGGACG AGTACCTGTA CCGGCTGCGC ACCCGTCACC TGAGCCAAAT CACGGAGGCT 
GCCCTGACCC TGAAGCTTGG CCACAGTGAG CTCCCTGCTG CTCTGGATCA GGCTGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTTGCTGAG GAGCCCCAGA ACAGCCTGCC GGATATCATC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGTT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC CAGACCAAGT TGGCCCTGGT CGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CCGGCAAGAT CAAGCTACCC 
AAGGAGAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCA 
GAGAAGACCC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTTGTGGA GGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACTGAT 
GTGAATGGGG AGAAGGTGCT TCCCAAAGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TACCCCCGGA CCGGAAGCCG AAGCACTGGG TCCCTGTTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAGATGGAA 
GCGCTGAAGA GGCATAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCATCT CGAGTACCGC AAGACAGACG CCTTCCGCCG CCGCCGCTGG 
CGCCGCCGCA TGGAGCCCCT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTCGAGGGG 
GCACTGGGCG GCGTGGTGGA CGACAAGAGT GAAGATTCCA TGTCCGCCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC AATTTCCTGC ATATTTGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGC GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT CGGCGAGCCG 
GCCACAGTTG CTGAGCAGCC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAC 
GGCGCAGACG AGTTTATGGG CCGCTGCATC TGTCAACCGA GTTTGGAACG GATGCCCCGA 
CTGGCCTGGT TCCCACTGAC CAGGGGCAGC CAGCCGATGG GGGAGCTGCT GGCCTCTTTT 
GAGCTTATCC AGAGAGAGAA GCCAGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCGAGGA TCCTGGATGA GTCTGAGGAC ACAGACTTGC CCTACCCACC ACCCCAAAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGGTAGAGTG TGGGGGCCAA ACGGTGCAGT CCTGTGTCAT TAGGAACCTC 
CGGAAGAATC CCAACTTTGA CATCTGTACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CGTCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCTAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCACGCAG 
GGACCGCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTAGA GAAGGACCTG AAGGTCACCC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC ACCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCATGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCGGC AGCAGGGCCT GGTCCCAGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCGTTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACGGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTACCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCTGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361945.2 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360247.2 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X13, mRNA.

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CloneID	ORh253201
Clone ID Related Accession (Same CDS sequence)	XM_010361945.2
Accession Version	XM_010361945.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 25, 2019 this sequence version replaced XM_010361945.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Rhinopithecus roxellana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGTGGAGGC 
CCAGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCTGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCGGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA AACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTCC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCACCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGAC TGCAGGTGCC 
GTCAAGTCTT CGAAAGCCGC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGTTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGACT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TCGAGGTCAG CATTGGGAAC 
TATGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GTAATGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGCTGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GACCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTTG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACCGGC AAGATCAAGC TACCCAAGGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCAGAGAA GACCCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CTGATGTGAA TGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATACCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GTTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CATCTCGAGT ACCGCAAGAC AGACGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCACT GGGCGGCGTG 
GTGGACGACA AGAGTGAAGA TTCCATGTCC GCCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGCGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTACGGCGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA ACCGAGTTTG GAACGGATGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GATGGGGGAG CTGCTGGCCT CTTTTGAGCT TATCCAGAGA 
GAGAAGCCAG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGTCTG AGGACACAGA CTTGCCCTAC CCACCACCCC AAAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATTGAGGT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAGCATCT CCTCCCCGAG CCTTGTGGTA 
GAGTGTGGGG GCCAAACGGT GCAGTCCTGT GTCATTAGGA ACCTCCGGAA GAATCCCAAC 
TTTGACATCT GTACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCA TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCGTCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGGAGGAAG AGTTTATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GCGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCACGC AGGGACCGCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGT TGGAGCCTGT GTTTGGAAAG ATGTTTGAGC TGACCTGCAC TCTGCCTCTA 
GAGAAGGACC TGAAGGTCAC CCTCTACGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGTGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCACCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAGG CGCCTGTGTA CCGGACAGAC 
CGTGTGATGT TTCAGGATAA AGAATACTCC ATTGAAGAAA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCCAT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCG GCAGCAGGGC 
CTGGTCCCAG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCGTTT TCCCCTTCGA CTACCTGCCG 
GCTGAGCAAG TCTGTACGGT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GATATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTACCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCCGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCTGACA CCTCCTTCCT GTGGTTCACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360247.2
CDS	139..6384
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEEETAGAVKSSKAADLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLHPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361945.2
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361945.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGTGGAGGC 
CCAGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCTGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCGGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA AACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTCC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCACCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGAC TGCAGGTGCC 
GTCAAGTCTT CGAAAGCCGC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGTTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGACT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TCGAGGTCAG CATTGGGAAC 
TATGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GTAATGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGCTGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GACCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTTG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACCGGC AAGATCAAGC TACCCAAGGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCAGAGAA GACCCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CTGATGTGAA TGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATACCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GTTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CATCTCGAGT ACCGCAAGAC AGACGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCACT GGGCGGCGTG 
GTGGACGACA AGAGTGAAGA TTCCATGTCC GCCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGCGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTACGGCGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA ACCGAGTTTG GAACGGATGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GATGGGGGAG CTGCTGGCCT CTTTTGAGCT TATCCAGAGA 
GAGAAGCCAG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGTCTG AGGACACAGA CTTGCCCTAC CCACCACCCC AAAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATTGAGGT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAGCATCT CCTCCCCGAG CCTTGTGGTA 
GAGTGTGGGG GCCAAACGGT GCAGTCCTGT GTCATTAGGA ACCTCCGGAA GAATCCCAAC 
TTTGACATCT GTACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCA TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCGTCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGGAGGAAG AGTTTATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GCGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCACGC AGGGACCGCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGT TGGAGCCTGT GTTTGGAAAG ATGTTTGAGC TGACCTGCAC TCTGCCTCTA 
GAGAAGGACC TGAAGGTCAC CCTCTACGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGTGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCACCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAGG CGCCTGTGTA CCGGACAGAC 
CGTGTGATGT TTCAGGATAA AGAATACTCC ATTGAAGAAA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCCAT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCG GCAGCAGGGC 
CTGGTCCCAG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCGTTT TCCCCTTCGA CTACCTGCCG 
GCTGAGCAAG TCTGTACGGT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GATATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTACCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCCGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCTGACA CCTCCTTCCT GTGGTTCACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361946.2 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360248.2 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X14, mRNA.

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CloneID	ORh253202
Clone ID Related Accession (Same CDS sequence)	XM_010361946.2
Accession Version	XM_010361946.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 25, 2019 this sequence version replaced XM_010361946.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Rhinopithecus roxellana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG TGGAGGCCCA 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCTGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCGGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAAAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTCCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CACCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGACTGC AGGTGCCGTC 
AAGTCTTCGA AAGCCGCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GTTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGACTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTCG AGGTCAGCAT TGGGAACTAT 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGTA ATGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGCTGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGAC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTTGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACCGGCAAG ATCAAGCTAC CCAAGGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CAGAGAAGAC CCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACTG ATGTGAATGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATACCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGTT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAT 
CTCGAGTACC GCAAGACAGA CGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCACTGGG CGGCGTGGTG 
GACGACAAGA GTGAAGATTC CATGTCCGCC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GCGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTCGGCGAGC CGGCCACAGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ACGGCGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAACC GAGTTTGGAA CGGATGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAT GGGGGAGCTG CTGGCCTCTT TTGAGCTTAT CCAGAGAGAG 
AAGCCAGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACTT GCCCTACCCA CCACCCCAAA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TTGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGGTAGAG 
TGTGGGGGCC AAACGGTGCA GTCCTGTGTC ATTAGGAACC TCCGGAAGAA TCCCAACTTT 
GACATCTGTA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCGTCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG GAGGAAGAGT TTATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACTTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTCATTGGCG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCACGCAGG GACCGCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGTTGG AGCCTGTGTT TGGAAAGATG TTTGAGCTGA CCTGCACTCT GCCTCTAGAG 
AAGGACCTGA AGGTCACCCT CTACGACTAT GACCTCCTCT CCAAGGATGA AAAGATTGGT 
GAGACGGTCA TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGCGGCTCG CTGTGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCA CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCGCAGA GTCAAGGCGC CTGTGTACCG GACAGACCGT 
GTGATGTTTC AGGATAAAGA ATACTCCATT GAAGAAATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCCATGGA GGAGCGTCTG GCTCTGCATG TGCTTCGGCA GCAGGGCCTG 
GTCCCAGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATTGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTGTTT CCGAAGGCCC TGGGGCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACC 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGCTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCGTTTTCC CCTTCGACTA CCTGCCGGCT 
GAGCAAGTCT GTACGGTTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGAT ATTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACT TACCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTAGCAG AAGAGGGTGA GAAGAAGATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAACAG AGAGTGAGCA TGAGGAGCGG 
CCCGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCTGACACCT CCTTCCTGTG GTTCACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360248.2
CDS	439..6681
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEEETAGAVKSSKAADLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLHPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361946.2
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361946.2

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG TGGAGGCCCA 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCTGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCGGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAAAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTCCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CACCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGACTGC AGGTGCCGTC 
AAGTCTTCGA AAGCCGCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GTTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGACTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTCG AGGTCAGCAT TGGGAACTAT 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGTA ATGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGCTGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGAC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTTGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACCGGCAAG ATCAAGCTAC CCAAGGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CAGAGAAGAC CCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACTG ATGTGAATGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATACCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGTT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAT 
CTCGAGTACC GCAAGACAGA CGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCACTGGG CGGCGTGGTG 
GACGACAAGA GTGAAGATTC CATGTCCGCC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GCGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTCGGCGAGC CGGCCACAGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ACGGCGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAACC GAGTTTGGAA CGGATGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAT GGGGGAGCTG CTGGCCTCTT TTGAGCTTAT CCAGAGAGAG 
AAGCCAGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACTT GCCCTACCCA CCACCCCAAA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TTGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGGTAGAG 
TGTGGGGGCC AAACGGTGCA GTCCTGTGTC ATTAGGAACC TCCGGAAGAA TCCCAACTTT 
GACATCTGTA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCGTCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG GAGGAAGAGT TTATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACTTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTCATTGGCG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCACGCAGG GACCGCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGTTGG AGCCTGTGTT TGGAAAGATG TTTGAGCTGA CCTGCACTCT GCCTCTAGAG 
AAGGACCTGA AGGTCACCCT CTACGACTAT GACCTCCTCT CCAAGGATGA AAAGATTGGT 
GAGACGGTCA TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGCGGCTCG CTGTGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCA CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCGCAGA GTCAAGGCGC CTGTGTACCG GACAGACCGT 
GTGATGTTTC AGGATAAAGA ATACTCCATT GAAGAAATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCCATGGA GGAGCGTCTG GCTCTGCATG TGCTTCGGCA GCAGGGCCTG 
GTCCCAGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATTGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTGTTT CCGAAGGCCC TGGGGCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACC 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGCTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCGTTTTCC CCTTCGACTA CCTGCCGGCT 
GAGCAAGTCT GTACGGTTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGAT ATTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACT TACCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTAGCAG AAGAGGGTGA GAAGAAGATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAACAG AGAGTGAGCA TGAGGAGCGG 
CCCGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCTGACACCT CCTTCCTGTG GTTCACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361947.2 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360249.2 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X15, mRNA.

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CloneID	ORh253203
Clone ID Related Accession (Same CDS sequence)	XM_010361947.2
Accession Version	XM_010361947.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 25, 2019 this sequence version replaced XM_010361947.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Rhinopithecus roxellana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGTGGAGGC 
CCAGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCTGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCGGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA AACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTCC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCACCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CCACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGTTTCCTGG ACCCCTACGC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGACTTC TGCTTTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC GGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTC 
GAGGTCAGCA TTGGGAACTA TGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ATAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AATGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CACTGGTGGC TCAGCTGATG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAT ACCCTCTGCC 
ACCCACCTGG ACGAGTACCT GTACCGGCTG CGCACCCGTC ACCTGAGCCA AATCACGGAG 
GCTGCCCTGA CCCTGAAGCT TGGCCACAGT GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTTGCT GAGGAGCCCC AGAACAGCCT GCCGGATATC 
ATCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GTTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACCAGACCA AGTTGGCCCT GGTCGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACCGGCAA GATCAAGCTA 
CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCAGAGAAGA CCCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTTGT GGAGGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACT 
GATGTGAATG GGGAGAAGGT GCTTCCCAAA GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATACCCCC GGACCGGAAG CCGAAGCACT GGGTCCCTGT TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAGATG 
GAAGCGCTGA AGAGGCATAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA TCTCGAGTAC CGCAAGACAG ACGCCTTCCG CCGCCGCCGC 
TGGCGCCGCC GCATGGAGCC CCTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG 
GGGGCACTGG GCGGCGTGGT GGACGACAAG AGTGAAGATT CCATGTCCGC CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACAATTTCC TGCATATTTG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGCGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTCGGCGAG 
CCGGCCACAG TTGCTGAGCA GCCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TACGGCGCAG ACGAGTTTAT GGGCCGCTGC ATCTGTCAAC CGAGTTTGGA ACGGATGCCC 
CGACTGGCCT GGTTCCCACT GACCAGGGGC AGCCAGCCGA TGGGGGAGCT GCTGGCCTCT 
TTTGAGCTTA TCCAGAGAGA GAAGCCAGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCGA GGATCCTGGA TGAGTCTGAG GACACAGACT TGCCCTACCC ACCACCCCAA 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATTGAGGTCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGCATCTCC 
TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC CAAACGGTGC AGTCCTGTGT CATTAGGAAC 
CTCCGGAAGA ATCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCATC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCGTCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCACGCAG 
GGACCGCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTAGA GAAGGACCTG AAGGTCACCC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC ACCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCATGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCGGC AGCAGGGCCT GGTCCCAGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCGTTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACGGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTACCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCTGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360249.2
CDS	139..6342
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLHPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361947.2
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361947.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGTGGAGGC 
CCAGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCTGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCGGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA AACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTCC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCACCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CCACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGTTTCCTGG ACCCCTACGC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGACTTC TGCTTTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC GGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTC 
GAGGTCAGCA TTGGGAACTA TGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ATAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AATGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CACTGGTGGC TCAGCTGATG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAT ACCCTCTGCC 
ACCCACCTGG ACGAGTACCT GTACCGGCTG CGCACCCGTC ACCTGAGCCA AATCACGGAG 
GCTGCCCTGA CCCTGAAGCT TGGCCACAGT GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTTGCT GAGGAGCCCC AGAACAGCCT GCCGGATATC 
ATCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GTTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACCAGACCA AGTTGGCCCT GGTCGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACCGGCAA GATCAAGCTA 
CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCAGAGAAGA CCCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTTGT GGAGGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACT 
GATGTGAATG GGGAGAAGGT GCTTCCCAAA GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATACCCCC GGACCGGAAG CCGAAGCACT GGGTCCCTGT TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAGATG 
GAAGCGCTGA AGAGGCATAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA TCTCGAGTAC CGCAAGACAG ACGCCTTCCG CCGCCGCCGC 
TGGCGCCGCC GCATGGAGCC CCTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG 
GGGGCACTGG GCGGCGTGGT GGACGACAAG AGTGAAGATT CCATGTCCGC CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACAATTTCC TGCATATTTG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGCGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTCGGCGAG 
CCGGCCACAG TTGCTGAGCA GCCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TACGGCGCAG ACGAGTTTAT GGGCCGCTGC ATCTGTCAAC CGAGTTTGGA ACGGATGCCC 
CGACTGGCCT GGTTCCCACT GACCAGGGGC AGCCAGCCGA TGGGGGAGCT GCTGGCCTCT 
TTTGAGCTTA TCCAGAGAGA GAAGCCAGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCGA GGATCCTGGA TGAGTCTGAG GACACAGACT TGCCCTACCC ACCACCCCAA 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATTGAGGTCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGCATCTCC 
TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC CAAACGGTGC AGTCCTGTGT CATTAGGAAC 
CTCCGGAAGA ATCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCATC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCGTCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCACGCAG 
GGACCGCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTAGA GAAGGACCTG AAGGTCACCC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC ACCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCATGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCGGC AGCAGGGCCT GGTCCCAGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCGTTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACGGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTACCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCTGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010361948.2 ) cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey) -> XP_010360250.2 Rhinopithecus roxellana dysferlin (DYSF), transcript variant X16, mRNA.

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CloneID	ORh253204
Clone ID Related Accession (Same CDS sequence)	XM_010361948.2
Accession Version	XM_010361948.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Product	dysferlin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 25, 2019 this sequence version replaced XM_010361948.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Rhinopithecus roxellana Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG TGGAGGCCCA 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCTGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCGGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAAAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTCCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CACCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACCA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGT 
TTCCTGGACC CCTACGCAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGACTTCTGC TTTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAGCAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCGGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTCGAG 
GTCAGCATTG GGAACTATGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTATA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGTAAT 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCAC TGGTGGCTCA GCTGATGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGATACC CTCTGCCACC 
CACCTGGACG AGTACCTGTA CCGGCTGCGC ACCCGTCACC TGAGCCAAAT CACGGAGGCT 
GCCCTGACCC TGAAGCTTGG CCACAGTGAG CTCCCTGCTG CTCTGGATCA GGCTGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTTGCTGAG GAGCCCCAGA ACAGCCTGCC GGATATCATC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGTT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC CAGACCAAGT TGGCCCTGGT CGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CCGGCAAGAT CAAGCTACCC 
AAGGAGAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCA 
GAGAAGACCC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTTGTGGA GGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACTGAT 
GTGAATGGGG AGAAGGTGCT TCCCAAAGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TACCCCCGGA CCGGAAGCCG AAGCACTGGG TCCCTGTTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAGATGGAA 
GCGCTGAAGA GGCATAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCATCT CGAGTACCGC AAGACAGACG CCTTCCGCCG CCGCCGCTGG 
CGCCGCCGCA TGGAGCCCCT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTCGAGGGG 
GCACTGGGCG GCGTGGTGGA CGACAAGAGT GAAGATTCCA TGTCCGCCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC AATTTCCTGC ATATTTGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGC GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT CGGCGAGCCG 
GCCACAGTTG CTGAGCAGCC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAC 
GGCGCAGACG AGTTTATGGG CCGCTGCATC TGTCAACCGA GTTTGGAACG GATGCCCCGA 
CTGGCCTGGT TCCCACTGAC CAGGGGCAGC CAGCCGATGG GGGAGCTGCT GGCCTCTTTT 
GAGCTTATCC AGAGAGAGAA GCCAGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCGAGGA TCCTGGATGA GTCTGAGGAC ACAGACTTGC CCTACCCACC ACCCCAAAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGGTAGAGTG TGGGGGCCAA ACGGTGCAGT CCTGTGTCAT TAGGAACCTC 
CGGAAGAATC CCAACTTTGA CATCTGTACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CGTCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTT 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT CATTGGCGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CACGCAGGGA 
CCGCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGTTGGAG CCTGTGTTTG GAAAGATGTT TGAGCTGACC 
TGCACTCTGC CTCTAGAGAA GGACCTGAAG GTCACCCTCT ACGACTATGA CCTCCTCTCC 
AAGGATGAAA AGATTGGTGA GACGGTCATC GACCTGGAGA ACAGGCTGCT GTCCAAGTTT 
GCGGCTCGCT GTGGACTCCC ACAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCACC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCGCAGAGT CAAGGCGCCT 
GTGTACCGGA CAGACCGTGT GATGTTTCAG GATAAAGAAT ACTCCATTGA AGAAATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCCATGGAGG AGCGTCTGGC TCTGCATGTG 
CTTCGGCAGC AGGGCCTGGT CCCAGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TTGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTGTTTCC GAAGGCCCTG 
GGGCGGCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TGTATTATCT GGAATACCAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGCTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CGTTTTCCCC 
TTCGACTACC TGCCGGCTGA GCAAGTCTGT ACGGTTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGATAT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGACACTTA CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCCTG TGTAGCAGAA 
GAGGGTGAGA AGAAGATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAACAGAG 
AGTGAGCATG AGGAGCGGCC CGCTGGCCAG GGCCGGGATG AGCCCAACAT GAACCCTAAG 
CTTGAGGACC CAAGGCGCCC TGACACCTCC TTCCTGTGGT TCACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTCAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010360250.2
CDS	439..6639
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTDLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALTLKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSASTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPMGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLHPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPMEERLALHVLRQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTYHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010361948.2
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)
Definition	Rhinopithecus roxellana dysferlin (DYSF), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010361948.2

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG TGGAGGCCCA 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCTGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCGGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAAAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTCCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CACCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACCA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGT 
TTCCTGGACC CCTACGCAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGACTTCTGC TTTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAGCAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCGGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTCGAG 
GTCAGCATTG GGAACTATGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTATA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGTAAT 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCAC TGGTGGCTCA GCTGATGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGATACC CTCTGCCACC 
CACCTGGACG AGTACCTGTA CCGGCTGCGC ACCCGTCACC TGAGCCAAAT CACGGAGGCT 
GCCCTGACCC TGAAGCTTGG CCACAGTGAG CTCCCTGCTG CTCTGGATCA GGCTGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTTGCTGAG GAGCCCCAGA ACAGCCTGCC GGATATCATC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGTT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC CAGACCAAGT TGGCCCTGGT CGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CCGGCAAGAT CAAGCTACCC 
AAGGAGAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCA 
GAGAAGACCC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTTGTGGA GGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACTGAT 
GTGAATGGGG AGAAGGTGCT TCCCAAAGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TACCCCCGGA CCGGAAGCCG AAGCACTGGG TCCCTGTTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAGATGGAA 
GCGCTGAAGA GGCATAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCATCT CGAGTACCGC AAGACAGACG CCTTCCGCCG CCGCCGCTGG 
CGCCGCCGCA TGGAGCCCCT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTCGAGGGG 
GCACTGGGCG GCGTGGTGGA CGACAAGAGT GAAGATTCCA TGTCCGCCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC AATTTCCTGC ATATTTGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGC GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT CGGCGAGCCG 
GCCACAGTTG CTGAGCAGCC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAC 
GGCGCAGACG AGTTTATGGG CCGCTGCATC TGTCAACCGA GTTTGGAACG GATGCCCCGA 
CTGGCCTGGT TCCCACTGAC CAGGGGCAGC CAGCCGATGG GGGAGCTGCT GGCCTCTTTT 
GAGCTTATCC AGAGAGAGAA GCCAGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCGAGGA TCCTGGATGA GTCTGAGGAC ACAGACTTGC CCTACCCACC ACCCCAAAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGGTAGAGTG TGGGGGCCAA ACGGTGCAGT CCTGTGTCAT TAGGAACCTC 
CGGAAGAATC CCAACTTTGA CATCTGTACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CGTCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTT 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT CATTGGCGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CACGCAGGGA 
CCGCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGTTGGAG CCTGTGTTTG GAAAGATGTT TGAGCTGACC 
TGCACTCTGC CTCTAGAGAA GGACCTGAAG GTCACCCTCT ACGACTATGA CCTCCTCTCC 
AAGGATGAAA AGATTGGTGA GACGGTCATC GACCTGGAGA ACAGGCTGCT GTCCAAGTTT 
GCGGCTCGCT GTGGACTCCC ACAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCACC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCGCAGAGT CAAGGCGCCT 
GTGTACCGGA CAGACCGTGT GATGTTTCAG GATAAAGAAT ACTCCATTGA AGAAATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCCATGGAGG AGCGTCTGGC TCTGCATGTG 
CTTCGGCAGC AGGGCCTGGT CCCAGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TTGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTGTTTCC GAAGGCCCTG 
GGGCGGCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TGTATTATCT GGAATACCAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGCTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CGTTTTCCCC 
TTCGACTACC TGCCGGCTGA GCAAGTCTGT ACGGTTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGATAT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGACACTTA CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCCTG TGTAGCAGAA 
GAGGGTGAGA AGAAGATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAACAGAG 
AGTGAGCATG AGGAGCGGCC CGCTGGCCAG GGCCGGGATG AGCCCAACAT GAACCCTAAG 
CTTGAGGACC CAAGGCGCCC TGACACCTCC TTCCTGTGGT TCACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTCAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DYSF
Entrez Gene ID	104661345
Full Name	dysferlin
Gene Type	protein-coding
Organism	Rhinopithecus roxellana(golden snub-nosed monkey)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DYSF cDNA ORF clone, Rhinopithecus roxellana(golden snub-nosed monkey)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: