Prkd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkd1
Entrez Gene ID	85421
Full Name	protein kinase D1
Synonyms	Pkcm,Prkcm,nPKC-D1,nPKC-mu
General protein information	Preferred Names protein kinase D1 Names serine/threonine-protein kinase D1 protein kinase C mu type
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q22
Summary	serine/threonine kinase; may play a role in the regulation of physiological events such as ion and protein secretion; may mediate cell proliferation or differentiation through participation in various signalling cascades [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017594389.1	XP_017449878.1	serine/threonine-protein kinase D1 isoform X2
NM_001276715.1	NP_001263644.1	serine/threonine-protein kinase D1
XM_006240074.3	XP_006240136.1	serine/threonine-protein kinase D1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PRKD1	XP_005623344.1
Danio rerio (zebrafish)	prkd1	XP_005158873.1
Homo sapiens (human)	PRKD1	NP_002733.2
Bos taurus (cattle)	PRKD1	XP_005222122.1
Xenopus tropicalis (tropical clawed frog)	prkd1	NP_001120884.1
Pan troglodytes (chimpanzee)	PRKD1	XP_003952507.1
Mus musculus (house mouse)	Prkd1	NP_032884.2
Gallus gallus (chicken)	PRKD1	NP_001026372.1
	PRKD1	XP_001114639.1
Rattus norvegicus (Norway rat)	Prkd1	NP_001263644.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential regulation of PKD isoforms in oxidative stress conditions through phosphorylation of a conserved Tyr in the P+1 loop.
Cobbaut M, Derua R, D?ppler H, Lou HJ, Vandoninck S, Storz P, Turk BE, Seufferlein T, Waelkens E, Janssens V, Van Lint J
Scientific reports7(1)887(2017 Apr)

Biphasic Regulation of Yes-associated Protein (YAP) Cellular Localization, Phosphorylation, and Activity by G Protein-coupled Receptor Agonists in Intestinal Epithelial Cells: A NOVEL ROLE FOR PROTEIN KINASE D (PKD).
Wang J, Sinnett-Smith J, Stevens JV, Young SH, Rozengurt E
The Journal of biological chemistry291(34)17988-8005(2016 08)

An Evolutionarily Conserved PLC-PKD-TFEB Pathway for Host Defense.
Najibi M, Labed SA, Visvikis O, Irazoqui JE
Cell reports15(8)1728-42(2016 05)

Heat shock protein 20 (HSP20) is a novel substrate for protein kinase D1 (PKD1).
Sin YY, Baillie GS
Cell biochemistry and function33(7)421-6(2015 Oct)

Protein kinase D1 mediates class IIa histone deacetylase phosphorylation and nuclear extrusion in intestinal epithelial cells: role in mitogenic signaling.
Sinnett-Smith J, Ni Y, Wang J, Ming M, Young SH, Rozengurt E
American journal of physiology. Cell physiology306(10)C961-71(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

siRNA-mediated knockdown of PKD1, PKD2, and PKD3 markedly attenuated YAP nuclear-cytoplasmic shuttling, phosphorylation at Ser(127), and induction of Ctgf and Areg expression in response to GPCR activation.
Title: Biphasic Regulation of Yes-associated Protein (YAP) Cellular Localization, Phosphorylation, and Activity by G Protein-coupled Receptor Agonists in Intestinal Epithelial Cells: A NOVEL ROLE FOR PROTEIN KINASE D (PKD).

Study delineates the protein kinase D1 binding sites on HSP20 and shows that HSP20 is a substrate for protein kinase D1.
Title: Heat shock protein 20 (HSP20) is a novel substrate for protein kinase D1 (PKD1).

PKD1 mediates class IIa histone deacetylase phosphorylation and nuclear extrusion in intestinal epithelial cells.
Title: Protein kinase D1 mediates class IIa histone deacetylase phosphorylation and nuclear extrusion in intestinal epithelial cells: role in mitogenic signaling.

Data suggest Fhl1 (four-and-a-half LIM domains protein 1) and FHL2 (four-and-a-half LIM domains protein 2) bind to/regulate activity of Prkd1 (protein kinase D 1) in cardiac/ventricular myocytes; knockdown of Fhl1/FHL2 downregulates Prkd1 activation.
Title: Four-and-a-half LIM domains proteins are novel regulators of the protein kinase D pathway in cardiac myocytes.

Overexpression of AMP-activated protein kinase or protein kinase D prevents lipid-induced insulin resistance in cardiomyocytes.
Title: Overexpression of AMP-activated protein kinase or protein kinase D prevents lipid-induced insulin resistance in cardiomyocytes.

The following Prkd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12662	NM_001276715.1 Latest version!	Rattus norvegicus protein kinase D1 (Prkd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
ORa29328	XM_006240074.3 Latest version!	Rattus norvegicus protein kinase D1 (Prkd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
ORa61778	XM_017594389.1 Latest version!	Rattus norvegicus protein kinase D1 (Prkd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkd1 ( NM_001276715.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001263644.1 Rattus norvegicus protein kinase D1 (Prkd1), mRNA.

Price & Availability↑

CloneID	ORa12662
Clone ID Related Accession (Same CDS sequence)	NM_001276715.1
Accession Version	NM_001276715.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase D1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07064252.1. On or before Mar 1, 2013 this sequence version replaced XM_001078506.3, XM_234108.6. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGAGCGCCC CTCCGCTACT ACGGCCGCCT AGCCCGCTGC TGCCCGCCGC GGCCGCTGTG 
GCCGCCGCCG CTGCTGCGCT GGTCCCGGGC TCCGGGCCCG CGCCCTTCCC AGCGCCTGGG 
GCCGCCCCGG CGGGGGGCAT CTCGTTCCAT CTGCAGATCG GCCTGAGCCG CGAGCCGGTG 
CTGCTCCTTC AGGACTCCTC TGGCGACTAC AGCCTGGCGC ATGTCCGGGA GATGGCTTGC 
TCCATCGTGG ACCAGAAGTT CCCTGAATGT GGCTTCTATG GACTCTATGA CAAGATCCTG 
CTTTTCCGTC ACGATCCTGC CTCTGAAAAC ATCCTTCAGC TGGTAAAAAT CGCAAGTGAC 
ATTCAGGAGG GCGATCTTAT TGAAGTGGTC CTGTCAGCTT CAGCCACCTT CGAAGACTTC 
CAGATCCGGC CTCACGCTCT CTTCGTTCAT TCATACAGAG CCCCTGCTTT CTGTGATCAC 
TGTGGAGAAA TGCTGTGGGG ACTGGTGCGC CAAGGCCTTA AATGTGAAGG ATGTGGCCTG 
AATTACCATA AGAGATGTGC ATTTAAAATC CCCAACAATT GCAGTGGAGT GAGAAGGAGA 
AGGCTCTCAA ATGTTTCCCT CACTGGACTG GGTACTGTCC GCACAGCGTC TGCGGAGTTC 
TCCACCAGTG CCCCCGATGA GCCTCTACTG TCTCCTGTGA GCCCTGGCTT TGAGCAAAAG 
TCTCCATCTG AGTCATTTAT CGGTCGTGAG AAGAGGTCAA ATTCTCAGTC ATATGTTGGA 
CGGCCGATTC AGCTCGACAA GCTCCTGATG TCTAAGGTGA AGGTGCCACA CACCTTTGTC 
ATCCACTCCT ACACACGGCC CACGGTCTGC CAGTTCTGTA AGAAGCTCCT CAAGGGCCTC 
TTCCGGCAGG GTTTGCAGTG CAAAGATTGC CGATTCAACT GTCACAAACG CTGTGCACCA 
AAAGTACCAA ACAACTGCTT GGGCGAAGTG ACCATCAATG GAGAATTGCT TAGCCCTGGG 
GCAGAGTCTG ATGTTGTCAT GGAAGAAGGG AGCGATGACA ATGACAGCGA ACGGAACAGT 
GGACTCATGG ATGACATGGA CGAGGCCATG GTCCAGGACA CTGAGATGGC TTTGGCGGAG 
GGTCAGAGTG ACGGTGCAGA GATGCAGGAT CCAGACGCAG ACCAGGAGGA CTCCAACAGA 
ACCATCAGCC CTTCTACGAG CAACAACATA CCGCTTATGA GGGTAGTGCA GTCTGTCAAG 
CACACAAAGA GGAGAAGCAG CACAGTGATG AAGGAAGGGT GGATGGTCCA CTACACCAGC 
AAGGACACAC TGAGGAAAAG GCATTACTGG AGACTGGACA GCAAAAGCAT CACACTCTTC 
CAAAACGACA CAGGCAGCCG GTACTACAAG GAAATTCCTT TATCAGAAAT TTTATGTCTG 
GAACCAGCAA AACCTTCAGC ATTAATTCCC ACTGGAGCTA ACCCTCATTG TTTTGAAATC 
ACTACAGCAA ACGTAGTGTA TTATGTGGGA GAAAACGTGG TCAACCCTTC AAGCCCCCCA 
CCAAACAACA GCGTCCCCCC GAGCGGCATC GGTACCGATG TGGCGAGGAT GTGGGAGGTC 
GCCATCCAGC ACGCTCTCAT GCCTGTCATC CCCAAGGGCT CCTCTGTGGG TTCCGGAACC 
AACTCACACA AAGATATTTC GGTGAGCATT TCCGTTTCGA ATAGCCAGAT TCAGGAAAAT 
GTGGATATCA GCACAGTCTA TCAGATTTTC CCGGATGAGG TTCTGGGTTC TGGACAGTTC 
GGAATTGTTT ATGGAGGTAA ACATCGTAAA ACAGGAAGAG ATGTAGCTAT TAAGATTATT 
GACAAATTAA GATTTCCAAC AAAACAAGAA AGTCAGCTTC GTAATGAGGT TGCAATTTTA 
CAGAACCTTC ATCACCCTGG TGTTGTAAAT TTGGAGTGTA TGTTTGAGAC GCCTGAAAGA 
GTGTTTGTTG TTATGGAAAA ACTCCATGGA GACATGCTGG AGATGATCCT GTCAAGTGAA 
AAGGGCAGGT TGCCAGAACA CATAACGAAG TTTTTAATTA CTCAGATACT AGTGGCTTTG 
CGACATCTTC ATTTTAAAAA CATCGTTCAC TGTGACCTCA AGCCAGAAAA TGTGTTGCTG 
GCATCAGCAG ACCCTTTCCC TCAGGTGAAA CTTTGTGATT TTGGTTTTGC CCGGATCATT 
GGCGAGAAGT CTTTCCGGAG ATCAGTGGTG GGTACCCCAG CCTACCTGGC ACCTGAGGTT 
CTGAGGAACA AGGGCTATAA CCGCTCGCTA GACATGTGGT CTGTTGGGGT CATCATCTAT 
GTGAGCCTGA GTGGCACCTT CCCTTTTAAT GAAGATGAAG ATATCCACGA TCAGATCCAG 
AACGCAGCCT TCATGTATCC ACCCAATCCC TGGAAGGAGA TTTCTCATGA AGCCATTGAT 
CTTATCAATA ACTTGCTACA AGTGAAAATG AGAAAACGCT ACAGTGTGGA CAAGACCTTG 
AGTCACCCCT GGCTACAGGA CTACCAGACC TGGTTAGATT TACGCGAGCT GGAATGCAGA 
ATTGGAGAAC GCTATATTAC CCATGAAAGC GATGACTCGA GGTGGGAACA GTATGCAGGC 
GAGCAGGGAC TGCAGTATCC CGCGCACCTG ATCAATCTGA GTGCTAGCCA TGGCGACAGT 
CCTGAGGCTG AAGAGAGAGA GATGAAAGCC CTCAGTGAGC GTGTCAGCAT CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263644.1
CDS	1..2757
Translation	MSAPPLLRPPSPLLPAAAAVAAAAAALVPGSGPAPFPAPGAAPAGGISFHLQIGLSREPVLLLQDSSGDYSLAHVREMACSIVDQKFPECGFYGLYDKILLFRHDPASENILQLVKIASDIQEGDLIEVVLSASATFEDFQIRPHALFVHSYRAPAFCDHCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRRRRLSNVSLTGLGTVRTASAEFSTSAPDEPLLSPVSPGFEQKSPSESFIGREKRSNSQSYVGRPIQLDKLLMSKVKVPHTFVIHSYTRPTVCQFCKKLLKGLFRQGLQCKDCRFNCHKRCAPKVPNNCLGEVTINGELLSPGAESDVVMEEGSDDNDSERNSGLMDDMDEAMVQDTEMALAEGQSDGAEMQDPDADQEDSNRTISPSTSNNIPLMRVVQSVKHTKRRSSTVMKEGWMVHYTSKDTLRKRHYWRLDSKSITLFQNDTGSRYYKEIPLSEILCLEPAKPSALIPTGANPHCFEITTANVVYYVGENVVNPSSPPPNNSVPPSGIGTDVARMWEVAIQHALMPVIPKGSSVGSGTNSHKDISVSISVSNSQIQENVDISTVYQIFPDEVLGSGQFGIVYGGKHRKTGRDVAIKIIDKLRFPTKQESQLRNEVAILQNLHHPGVVNLECMFETPERVFVVMEKLHGDMLEMILSSEKGRLPEHITKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLRNKGYNRSLDMWSVGVIIYVSLSGTFPFNEDEDIHDQIQNAAFMYPPNPWKEISHEAIDLINNLLQVKMRKRYSVDKTLSHPWLQDYQTWLDLRELECRIGERYITHESDDSRWEQYAGEQGLQYPAHLINLSASHGDSPEAEEREMKALSERVSIL

Target ORF information:

RefSeq Version	NM_001276715.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase D1 (Prkd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276715.1

ORF Insert Sequence:

ATGAGCGCCC CTCCGCTACT ACGGCCGCCT AGCCCGCTGC TGCCCGCCGC GGCCGCTGTG 
GCCGCCGCCG CTGCTGCGCT GGTCCCGGGC TCCGGGCCCG CGCCCTTCCC AGCGCCTGGG 
GCCGCCCCGG CGGGGGGCAT CTCGTTCCAT CTGCAGATCG GCCTGAGCCG CGAGCCGGTG 
CTGCTCCTTC AGGACTCCTC TGGCGACTAC AGCCTGGCGC ATGTCCGGGA GATGGCTTGC 
TCCATCGTGG ACCAGAAGTT CCCTGAATGT GGCTTCTATG GACTCTATGA CAAGATCCTG 
CTTTTCCGTC ACGATCCTGC CTCTGAAAAC ATCCTTCAGC TGGTAAAAAT CGCAAGTGAC 
ATTCAGGAGG GCGATCTTAT TGAAGTGGTC CTGTCAGCTT CAGCCACCTT CGAAGACTTC 
CAGATCCGGC CTCACGCTCT CTTCGTTCAT TCATACAGAG CCCCTGCTTT CTGTGATCAC 
TGTGGAGAAA TGCTGTGGGG ACTGGTGCGC CAAGGCCTTA AATGTGAAGG ATGTGGCCTG 
AATTACCATA AGAGATGTGC ATTTAAAATC CCCAACAATT GCAGTGGAGT GAGAAGGAGA 
AGGCTCTCAA ATGTTTCCCT CACTGGACTG GGTACTGTCC GCACAGCGTC TGCGGAGTTC 
TCCACCAGTG CCCCCGATGA GCCTCTACTG TCTCCTGTGA GCCCTGGCTT TGAGCAAAAG 
TCTCCATCTG AGTCATTTAT CGGTCGTGAG AAGAGGTCAA ATTCTCAGTC ATATGTTGGA 
CGGCCGATTC AGCTCGACAA GCTCCTGATG TCTAAGGTGA AGGTGCCACA CACCTTTGTC 
ATCCACTCCT ACACACGGCC CACGGTCTGC CAGTTCTGTA AGAAGCTCCT CAAGGGCCTC 
TTCCGGCAGG GTTTGCAGTG CAAAGATTGC CGATTCAACT GTCACAAACG CTGTGCACCA 
AAAGTACCAA ACAACTGCTT GGGCGAAGTG ACCATCAATG GAGAATTGCT TAGCCCTGGG 
GCAGAGTCTG ATGTTGTCAT GGAAGAAGGG AGCGATGACA ATGACAGCGA ACGGAACAGT 
GGACTCATGG ATGACATGGA CGAGGCCATG GTCCAGGACA CTGAGATGGC TTTGGCGGAG 
GGTCAGAGTG ACGGTGCAGA GATGCAGGAT CCAGACGCAG ACCAGGAGGA CTCCAACAGA 
ACCATCAGCC CTTCTACGAG CAACAACATA CCGCTTATGA GGGTAGTGCA GTCTGTCAAG 
CACACAAAGA GGAGAAGCAG CACAGTGATG AAGGAAGGGT GGATGGTCCA CTACACCAGC 
AAGGACACAC TGAGGAAAAG GCATTACTGG AGACTGGACA GCAAAAGCAT CACACTCTTC 
CAAAACGACA CAGGCAGCCG GTACTACAAG GAAATTCCTT TATCAGAAAT TTTATGTCTG 
GAACCAGCAA AACCTTCAGC ATTAATTCCC ACTGGAGCTA ACCCTCATTG TTTTGAAATC 
ACTACAGCAA ACGTAGTGTA TTATGTGGGA GAAAACGTGG TCAACCCTTC AAGCCCCCCA 
CCAAACAACA GCGTCCCCCC GAGCGGCATC GGTACCGATG TGGCGAGGAT GTGGGAGGTC 
GCCATCCAGC ACGCTCTCAT GCCTGTCATC CCCAAGGGCT CCTCTGTGGG TTCCGGAACC 
AACTCACACA AAGATATTTC GGTGAGCATT TCCGTTTCGA ATAGCCAGAT TCAGGAAAAT 
GTGGATATCA GCACAGTCTA TCAGATTTTC CCGGATGAGG TTCTGGGTTC TGGACAGTTC 
GGAATTGTTT ATGGAGGTAA ACATCGTAAA ACAGGAAGAG ATGTAGCTAT TAAGATTATT 
GACAAATTAA GATTTCCAAC AAAACAAGAA AGTCAGCTTC GTAATGAGGT TGCAATTTTA 
CAGAACCTTC ATCACCCTGG TGTTGTAAAT TTGGAGTGTA TGTTTGAGAC GCCTGAAAGA 
GTGTTTGTTG TTATGGAAAA ACTCCATGGA GACATGCTGG AGATGATCCT GTCAAGTGAA 
AAGGGCAGGT TGCCAGAACA CATAACGAAG TTTTTAATTA CTCAGATACT AGTGGCTTTG 
CGACATCTTC ATTTTAAAAA CATCGTTCAC TGTGACCTCA AGCCAGAAAA TGTGTTGCTG 
GCATCAGCAG ACCCTTTCCC TCAGGTGAAA CTTTGTGATT TTGGTTTTGC CCGGATCATT 
GGCGAGAAGT CTTTCCGGAG ATCAGTGGTG GGTACCCCAG CCTACCTGGC ACCTGAGGTT 
CTGAGGAACA AGGGCTATAA CCGCTCGCTA GACATGTGGT CTGTTGGGGT CATCATCTAT 
GTGAGCCTGA GTGGCACCTT CCCTTTTAAT GAAGATGAAG ATATCCACGA TCAGATCCAG 
AACGCAGCCT TCATGTATCC ACCCAATCCC TGGAAGGAGA TTTCTCATGA AGCCATTGAT 
CTTATCAATA ACTTGCTACA AGTGAAAATG AGAAAACGCT ACAGTGTGGA CAAGACCTTG 
AGTCACCCCT GGCTACAGGA CTACCAGACC TGGTTAGATT TACGCGAGCT GGAATGCAGA 
ATTGGAGAAC GCTATATTAC CCATGAAAGC GATGACTCGA GGTGGGAACA GTATGCAGGC 
GAGCAGGGAC TGCAGTATCC CGCGCACCTG ATCAATCTGA GTGCTAGCCA TGGCGACAGT 
CCTGAGGCTG AAGAGAGAGA GATGAAAGCC CTCAGTGAGC GTGTCAGCAT CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkd1 ( XM_006240074.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240136.1 Rattus norvegicus protein kinase D1 (Prkd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa29328
Clone ID Related Accession (Same CDS sequence)	XM_006240074.3
Accession Version	XM_006240074.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase D1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240074.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCCC CTCCGCTACT ACGGCCGCCT AGCCCGCTGC TGCCCGCCGC GGCCGCTGTG 
GCCGCCGCCG CTGCTGCGCT GGTCCCGGGC TCCGGGCCCG CGCCCTTCCC AGCGCCTGGG 
GCCGCCCCGG CGGGGGGCAT CTCGTTCCAT CTGCAGATCG GCCTGAGCCG CGAGCCGGTG 
CTGCTCCTTC AGGACTCCTC TGGCGACTAC AGCCTGGCGC ATGTCCGGGA GATGGCTTGC 
TCCATCGTGG ACCAGAAGTT CCCTGAATGT GGCTTCTATG GACTCTATGA CAAGATCCTG 
CTTTTCCGTC ACGATCCTGC CTCTGAAAAC ATCCTTCAGC TGGTAAAAAT CGCAAGTGAC 
ATTCAGGAGG GCGATCTTAT TGAAGTGGTC CTGTCAGCTT CAGCCACCTT CGAAGACTTC 
CAGATCCGGC CTCACGCTCT CTTCGTTCAT TCATACAGAG CCCCTGCTTT CTGTGATCAC 
TGTGGAGAAA TGCTGTGGGG ACTGGTGCGC CAAGGCCTTA AATGTGAAGG ATGTGGCCTG 
AATTACCATA AGAGATGTGC ATTTAAAATC CCCAACAATT GCAGTGGAGT GAGAAGGAGA 
AGGCTCTCAA ATGTTTCCCT CACTGGACTG GGTACTGTCC GCACAGCGTC TGCGGAGTTC 
TCCACCAGTG CCCCCGATGA GCCTCTACTG CAAAAGTCTC CATCTGAGTC ATTTATCGGT 
CGTGAGAAGA GGTCAAATTC TCAGTCATAT GTTGGACGGC CGATTCAGCT CGACAAGCTC 
CTGATGTCTA AGGTGAAGGT GCCACACACC TTTGTCATCC ACTCCTACAC ACGGCCCACG 
GTCTGCCAGT TCTGTAAGAA GCTCCTCAAG GGCCTCTTCC GGCAGGGTTT GCAGTGCAAA 
GATTGCCGAT TCAACTGTCA CAAACGCTGT GCACCAAAAG TACCAAACAA CTGCTTGGGC 
GAAGTGACCA TCAATGGAGA ATTGCTTAGC CCTGGGGCAG AGTCTGATGT TGTCATGGAA 
GAAGGGAGCG ATGACAATGA CAGCGAACGG AACAGTGGAC TCATGGATGA CATGGACGAG 
GCCATGGTCC AGGACACTGA GATGGCTTTG GCGGAGGGTC AGAGTGACGG TGCAGAGATG 
CAGGATCCAG ACGCAGACCA GGAGGACTCC AACAGAACCA TCAGCCCTTC TACGAGCAAC 
AACATACCGC TTATGAGGGT AGTGCAGTCT GTCAAGCACA CAAAGAGGAG AAGCAGCACA 
GTGATGAAGG AAGGGTGGAT GGTCCACTAC ACCAGCAAGG ACACACTGAG GAAAAGGCAT 
TACTGGAGAC TGGACAGCAA AAGCATCACA CTCTTCCAAA ACGACACAGG CAGCCGGTAC 
TACAAGGAAA TTCCTTTATC AGAAATTTTA TGTCTGGAAC CAGCAAAACC TTCAGCATTA 
ATTCCCACTG GAGCTAACCC TCATTGTTTT GAAATCACTA CAGCAAACGT AGTGTATTAT 
GTGGGAGAAA ACGTGGTCAA CCCTTCAAGC CCCCCACCAA ACAACAGCGT CCCCCCGAGC 
GGCATCGGTA CCGATGTGGC GAGGATGTGG GAGGTCGCCA TCCAGCACGC TCTCATGCCT 
GTCATCCCCA AGGGCTCCTC TGTGGGTTCC GGAACCAACT CACACAAAGA TATTTCGGTG 
AGCATTTCCG TTTCGAATAG CCAGATTCAG GAAAATGTGG ATATCAGCAC AGTCTATCAG 
ATTTTCCCGG ATGAGGTTCT GGGTTCTGGA CAGTTCGGAA TTGTTTATGG AGGTAAACAT 
CGTAAAACAG GAAGAGATGT AGCTATTAAG ATTATTGACA AATTAAGATT TCCAACAAAA 
CAAGAAAGTC AGCTTCGTAA TGAGGTTGCA ATTTTACAGA ACCTTCATCA CCCTGGTGTT 
GTAAATTTGG AGTGTATGTT TGAGACGCCT GAAAGAGTGT TTGTTGTTAT GGAAAAACTC 
CATGGAGACA TGCTGGAGAT GATCCTGTCA AGTGAAAAGG GCAGGTTGCC AGAACACATA 
ACGAAGTTTT TAATTACTCA GATACTAGTG GCTTTGCGAC ATCTTCATTT TAAAAACATC 
GTTCACTGTG ACCTCAAGCC AGAAAATGTG TTGCTGGCAT CAGCAGACCC TTTCCCTCAG 
GTGAAACTTT GTGATTTTGG TTTTGCCCGG ATCATTGGCG AGAAGTCTTT CCGGAGATCA 
GTGGTGGGTA CCCCAGCCTA CCTGGCACCT GAGGTTCTGA GGAACAAGGG CTATAACCGC 
TCGCTAGACA TGTGGTCTGT TGGGGTCATC ATCTATGTGA GCCTGAGTGG CACCTTCCCT 
TTTAATGAAG ATGAAGATAT CCACGATCAG ATCCAGAACG CAGCCTTCAT GTATCCACCC 
AATCCCTGGA AGGAGATTTC TCATGAAGCC ATTGATCTTA TCAATAACTT GCTACAAGTG 
AAAATGAGAA AACGCTACAG TGTGGACAAG ACCTTGAGTC ACCCCTGGCT ACAGGACTAC 
CAGACCTGGT TAGATTTACG CGAGCTGGAA TGCAGAATTG GAGAACGCTA TATTACCCAT 
GAAAGCGATG ACTCGAGGTG GGAACAGTAT GCAGGCGAGC AGGGACTGCA GTATCCCGCG 
CACCTGATCA ATCTGAGTGC TAGCCATGGC GACAGTCCTG AGGCTGAAGA GAGAGAGATG 
AAAGCCCTCA GTGAGCGTGT CAGCATCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240136.1
CDS	281..3013
Translation	MSAPPLLRPPSPLLPAAAAVAAAAAALVPGSGPAPFPAPGAAPAGGISFHLQIGLSREPVLLLQDSSGDYSLAHVREMACSIVDQKFPECGFYGLYDKILLFRHDPASENILQLVKIASDIQEGDLIEVVLSASATFEDFQIRPHALFVHSYRAPAFCDHCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRRRRLSNVSLTGLGTVRTASAEFSTSAPDEPLLQKSPSESFIGREKRSNSQSYVGRPIQLDKLLMSKVKVPHTFVIHSYTRPTVCQFCKKLLKGLFRQGLQCKDCRFNCHKRCAPKVPNNCLGEVTINGELLSPGAESDVVMEEGSDDNDSERNSGLMDDMDEAMVQDTEMALAEGQSDGAEMQDPDADQEDSNRTISPSTSNNIPLMRVVQSVKHTKRRSSTVMKEGWMVHYTSKDTLRKRHYWRLDSKSITLFQNDTGSRYYKEIPLSEILCLEPAKPSALIPTGANPHCFEITTANVVYYVGENVVNPSSPPPNNSVPPSGIGTDVARMWEVAIQHALMPVIPKGSSVGSGTNSHKDISVSISVSNSQIQENVDISTVYQIFPDEVLGSGQFGIVYGGKHRKTGRDVAIKIIDKLRFPTKQESQLRNEVAILQNLHHPGVVNLECMFETPERVFVVMEKLHGDMLEMILSSEKGRLPEHITKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLRNKGYNRSLDMWSVGVIIYVSLSGTFPFNEDEDIHDQIQNAAFMYPPNPWKEISHEAIDLINNLLQVKMRKRYSVDKTLSHPWLQDYQTWLDLRELECRIGERYITHESDDSRWEQYAGEQGLQYPAHLINLSASHGDSPEAEEREMKALSERVSIL

Target ORF information:

RefSeq Version	XM_006240074.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase D1 (Prkd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240074.3

ORF Insert Sequence:

ATGAGCGCCC CTCCGCTACT ACGGCCGCCT AGCCCGCTGC TGCCCGCCGC GGCCGCTGTG 
GCCGCCGCCG CTGCTGCGCT GGTCCCGGGC TCCGGGCCCG CGCCCTTCCC AGCGCCTGGG 
GCCGCCCCGG CGGGGGGCAT CTCGTTCCAT CTGCAGATCG GCCTGAGCCG CGAGCCGGTG 
CTGCTCCTTC AGGACTCCTC TGGCGACTAC AGCCTGGCGC ATGTCCGGGA GATGGCTTGC 
TCCATCGTGG ACCAGAAGTT CCCTGAATGT GGCTTCTATG GACTCTATGA CAAGATCCTG 
CTTTTCCGTC ACGATCCTGC CTCTGAAAAC ATCCTTCAGC TGGTAAAAAT CGCAAGTGAC 
ATTCAGGAGG GCGATCTTAT TGAAGTGGTC CTGTCAGCTT CAGCCACCTT CGAAGACTTC 
CAGATCCGGC CTCACGCTCT CTTCGTTCAT TCATACAGAG CCCCTGCTTT CTGTGATCAC 
TGTGGAGAAA TGCTGTGGGG ACTGGTGCGC CAAGGCCTTA AATGTGAAGG ATGTGGCCTG 
AATTACCATA AGAGATGTGC ATTTAAAATC CCCAACAATT GCAGTGGAGT GAGAAGGAGA 
AGGCTCTCAA ATGTTTCCCT CACTGGACTG GGTACTGTCC GCACAGCGTC TGCGGAGTTC 
TCCACCAGTG CCCCCGATGA GCCTCTACTG CAAAAGTCTC CATCTGAGTC ATTTATCGGT 
CGTGAGAAGA GGTCAAATTC TCAGTCATAT GTTGGACGGC CGATTCAGCT CGACAAGCTC 
CTGATGTCTA AGGTGAAGGT GCCACACACC TTTGTCATCC ACTCCTACAC ACGGCCCACG 
GTCTGCCAGT TCTGTAAGAA GCTCCTCAAG GGCCTCTTCC GGCAGGGTTT GCAGTGCAAA 
GATTGCCGAT TCAACTGTCA CAAACGCTGT GCACCAAAAG TACCAAACAA CTGCTTGGGC 
GAAGTGACCA TCAATGGAGA ATTGCTTAGC CCTGGGGCAG AGTCTGATGT TGTCATGGAA 
GAAGGGAGCG ATGACAATGA CAGCGAACGG AACAGTGGAC TCATGGATGA CATGGACGAG 
GCCATGGTCC AGGACACTGA GATGGCTTTG GCGGAGGGTC AGAGTGACGG TGCAGAGATG 
CAGGATCCAG ACGCAGACCA GGAGGACTCC AACAGAACCA TCAGCCCTTC TACGAGCAAC 
AACATACCGC TTATGAGGGT AGTGCAGTCT GTCAAGCACA CAAAGAGGAG AAGCAGCACA 
GTGATGAAGG AAGGGTGGAT GGTCCACTAC ACCAGCAAGG ACACACTGAG GAAAAGGCAT 
TACTGGAGAC TGGACAGCAA AAGCATCACA CTCTTCCAAA ACGACACAGG CAGCCGGTAC 
TACAAGGAAA TTCCTTTATC AGAAATTTTA TGTCTGGAAC CAGCAAAACC TTCAGCATTA 
ATTCCCACTG GAGCTAACCC TCATTGTTTT GAAATCACTA CAGCAAACGT AGTGTATTAT 
GTGGGAGAAA ACGTGGTCAA CCCTTCAAGC CCCCCACCAA ACAACAGCGT CCCCCCGAGC 
GGCATCGGTA CCGATGTGGC GAGGATGTGG GAGGTCGCCA TCCAGCACGC TCTCATGCCT 
GTCATCCCCA AGGGCTCCTC TGTGGGTTCC GGAACCAACT CACACAAAGA TATTTCGGTG 
AGCATTTCCG TTTCGAATAG CCAGATTCAG GAAAATGTGG ATATCAGCAC AGTCTATCAG 
ATTTTCCCGG ATGAGGTTCT GGGTTCTGGA CAGTTCGGAA TTGTTTATGG AGGTAAACAT 
CGTAAAACAG GAAGAGATGT AGCTATTAAG ATTATTGACA AATTAAGATT TCCAACAAAA 
CAAGAAAGTC AGCTTCGTAA TGAGGTTGCA ATTTTACAGA ACCTTCATCA CCCTGGTGTT 
GTAAATTTGG AGTGTATGTT TGAGACGCCT GAAAGAGTGT TTGTTGTTAT GGAAAAACTC 
CATGGAGACA TGCTGGAGAT GATCCTGTCA AGTGAAAAGG GCAGGTTGCC AGAACACATA 
ACGAAGTTTT TAATTACTCA GATACTAGTG GCTTTGCGAC ATCTTCATTT TAAAAACATC 
GTTCACTGTG ACCTCAAGCC AGAAAATGTG TTGCTGGCAT CAGCAGACCC TTTCCCTCAG 
GTGAAACTTT GTGATTTTGG TTTTGCCCGG ATCATTGGCG AGAAGTCTTT CCGGAGATCA 
GTGGTGGGTA CCCCAGCCTA CCTGGCACCT GAGGTTCTGA GGAACAAGGG CTATAACCGC 
TCGCTAGACA TGTGGTCTGT TGGGGTCATC ATCTATGTGA GCCTGAGTGG CACCTTCCCT 
TTTAATGAAG ATGAAGATAT CCACGATCAG ATCCAGAACG CAGCCTTCAT GTATCCACCC 
AATCCCTGGA AGGAGATTTC TCATGAAGCC ATTGATCTTA TCAATAACTT GCTACAAGTG 
AAAATGAGAA AACGCTACAG TGTGGACAAG ACCTTGAGTC ACCCCTGGCT ACAGGACTAC 
CAGACCTGGT TAGATTTACG CGAGCTGGAA TGCAGAATTG GAGAACGCTA TATTACCCAT 
GAAAGCGATG ACTCGAGGTG GGAACAGTAT GCAGGCGAGC AGGGACTGCA GTATCCCGCG 
CACCTGATCA ATCTGAGTGC TAGCCATGGC GACAGTCCTG AGGCTGAAGA GAGAGAGATG 
AAAGCCCTCA GTGAGCGTGT CAGCATCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkd1 ( XM_017594389.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449878.1 Rattus norvegicus protein kinase D1 (Prkd1), transcript variant X2, mRNA.

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CloneID	ORa61778
Clone ID Related Accession (Same CDS sequence)	XM_017594389.1
Accession Version	XM_017594389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase D1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCCC CTCCGCTACT ACGGCCGCCT AGCCCGCTGC TGCCCGCCGC GGCCGCTGTG 
GCCGCCGCCG CTGCTGCGCT GGTCCCGGGC TCCGGGCCCG CGCCCTTCCC AGCGCCTGGG 
GCCGCCCCGG CGGGGGGCAT CTCGTTCCAT CTGCAGATCG GCCTGAGCCG CGAGCCGGTG 
CTGCTCCTTC AGGACTCCTC TGGCGACTAC AGCCTGGCGC ATGTCCGGGA GATGGCTTGC 
TCCATCGTGG ACCAGAAGTT CCCTGAATGT GGCTTCTATG GACTCTATGA CAAGATCCTG 
CTTTTCCGTC ACGATCCTGC CTCTGAAAAC ATCCTTCAGC TGGTAAAAAT CGCAAGTGAC 
ATTCAGGAGG GCGATCTTAT TGAAGTGGTC CTGTCAGCTT CAGCCACCTT CGAAGACTTC 
CAGATCCGGC CTCACGCTCT CTTCGTTCAT TCATACAGAG CCCCTGCTTT CTGTGATCAC 
TGTGGAGAAA TGCTGTGGGG ACTGGTGCGC CAAGGCCTTA AATGTGAAGG ATGTGGCCTG 
AATTACCATA AGAGATGTGC ATTTAAAATC CCCAACAATT GCAGTGGAGT GAGAAGGAGA 
AGGCTCTCAA ATGTTTCCCT CACTGGACTG GGTACTGTCC GCACAGCGTC TGCGGAGTTC 
TCCACCAGTG CCCCCGATGA GCCTCTACTG TCTCCTGTGA GCCCTGGCTT TGAGCAAAAG 
TCTCCATCTG AGTCATTTAT CGGTCGTGAG AAGAGGTCAA ATTCTCAGTC ATATGTTGGA 
CGGCCGATTC AGCTCGACAA GCTCCTGATG TCTAAGGTGA AGGTGCCACA CACCTTTGTC 
ATCCACTCCT ACACACGGCC CACGGTCTGC CAGTTCTGTA AGAAGCTCCT CAAGGGCCTC 
TTCCGGCAGG GTTTGCAGTG CAAAGATTGC CGATTCAACT GTCACAAACG CTGTGCACCA 
AAAGTACCAA ACAACTGCTT GGGCGAAGTG ACCATCAATG GAGAATTGCT TAGCCCTGGG 
GCAGAGTCTG ATGTTGTCAT GGAAGAAGGG AGCGATGACA ATGACAGCGA ACGGAACAGT 
GGACTCATGG ATGACATGGA CGAGGCCATG GTCCAGGACA CTGAGATGGC TTTGGCGGAG 
GGTCAGAGTG ACGGTGCAGA GATGCAGGAT CCAGACGCAG ACCAGGAGGA CTCCAACAGA 
ACCATCAGCC CTTCTACGAG CAACAACATA CCGCTTATGA GGGTAGTGCA GTCTGTCAAG 
CACACAAAGA GGAGAAGCAG CACAGTGATG AAGGAAGGGT GGATGGTCCA CTACACCAGC 
AAGGACACAC TGAGGAAAAG GCATTACTGG AGACTGGACA GCAAAAGCAT CACACTCTTC 
CAAAACGACA CAGGCAGCCG GTACTACAAG GAAATTCCTT TATCAGAAAT TTTATGTCTG 
GAACCAGCAA AACCTTCAGC ATTAATTCCC ACTGGAGCTA ACCCTCATTG TTTTGAAATC 
ACTACAGCAA ACGTAGTGTA TTATGTGGGA GAAAACGTGG TCAACCCTTC AAGCCCCCCA 
CCAAACAACA GCGTCCCCCC GAGCGGCATC GGTACCGATG TGGCGAGGAT GTGGGAGGTC 
GCCATCCAGC ACGCTCTCAT GCCTGTCATC CCCAAGGGCT CCTCTGTGGG TTCCGGAACC 
AACTCACACA AAGATATTTC GGTGAGCATT TCCGTTTCGA ATAGCCAGAT TCAGGAAAAT 
GTGGATATCA GCACAGTCTA TCAGATTTTC CCGGATGAGG TTCTGGGTTC TGGACAGTTC 
GGAATTGTTT ATGGAGGTAA ACATCGTAAA ACAGGAAGAG ATGTAGCTAT TAAGATTATT 
GACAAATTAA GATTTCCAAC AAAACAAGAA AGTCAGCTTC GTAATGAGGT TGCAATTTTA 
CAGAACCTTC ATCACCCTGG TGTTGTAAAT TTGGAGTGTA TGTTTGAGAC GCCTGAAAGA 
GTGTTTGTTG TTATGGAAAA ACTCCATGGA GACATGCTGG AGATGATCCT GTCAAGTGAA 
AAGGGCAGGT TGCCAGAACA CATAACGAAG TTTTTAATTA CTCAGATACT AGTGGCTTTG 
CGACATCTTC ATTTTAAAAA CATCGTTCAC TGTGACCTCA AGCCAGAAAA TGTGTTGCTG 
GCATCAGCAG ACCCTTTCCC TCAGGTGAAA CTTTGTGATT TTGGTTTTGC CCGGATCATT 
GGCGAGAAGT CTTTCCGGAG ATCAGTGGTG GGTACCCCAG CCTACCTGGC ACCTGAGGTT 
CTGAGGAACA AGGGCTATAA CCGCTCGCTA GACATGTGGT CTGTTGGGGT CATCATCTAT 
GTGAGCCTGA GTGGCACCTT CCCTTTTAAT GAAGATGAAG ATATCCACGA TCAGATCCAG 
AACGCAGCCT TCATGTATCC ACCCAATCCC TGGAAGGAGA TTTCTCATGA AGATGACCAA 
ATGTTGAAAG TCAACTTGGG CTACATAGTA AAACTATACA ATAGTGATGA TGATGATGAT 
GATGATGATG ATGATGATGA CGACGACGAC GACGACGACG ACGACGACGA CGACGACAAC 
GACAACGACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449878.1
CDS	283..2877
Translation	MSAPPLLRPPSPLLPAAAAVAAAAAALVPGSGPAPFPAPGAAPAGGISFHLQIGLSREPVLLLQDSSGDYSLAHVREMACSIVDQKFPECGFYGLYDKILLFRHDPASENILQLVKIASDIQEGDLIEVVLSASATFEDFQIRPHALFVHSYRAPAFCDHCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRRRRLSNVSLTGLGTVRTASAEFSTSAPDEPLLSPVSPGFEQKSPSESFIGREKRSNSQSYVGRPIQLDKLLMSKVKVPHTFVIHSYTRPTVCQFCKKLLKGLFRQGLQCKDCRFNCHKRCAPKVPNNCLGEVTINGELLSPGAESDVVMEEGSDDNDSERNSGLMDDMDEAMVQDTEMALAEGQSDGAEMQDPDADQEDSNRTISPSTSNNIPLMRVVQSVKHTKRRSSTVMKEGWMVHYTSKDTLRKRHYWRLDSKSITLFQNDTGSRYYKEIPLSEILCLEPAKPSALIPTGANPHCFEITTANVVYYVGENVVNPSSPPPNNSVPPSGIGTDVARMWEVAIQHALMPVIPKGSSVGSGTNSHKDISVSISVSNSQIQENVDISTVYQIFPDEVLGSGQFGIVYGGKHRKTGRDVAIKIIDKLRFPTKQESQLRNEVAILQNLHHPGVVNLECMFETPERVFVVMEKLHGDMLEMILSSEKGRLPEHITKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLRNKGYNRSLDMWSVGVIIYVSLSGTFPFNEDEDIHDQIQNAAFMYPPNPWKEISHEDDQMLKVNLGYIVKLYNSDDDDDDDDDDDDDDDDDDDDDDDDNDNDN

Target ORF information:

RefSeq Version	XM_017594389.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase D1 (Prkd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594389.1

ORF Insert Sequence:

ATGAGCGCCC CTCCGCTACT ACGGCCGCCT AGCCCGCTGC TGCCCGCCGC GGCCGCTGTG 
GCCGCCGCCG CTGCTGCGCT GGTCCCGGGC TCCGGGCCCG CGCCCTTCCC AGCGCCTGGG 
GCCGCCCCGG CGGGGGGCAT CTCGTTCCAT CTGCAGATCG GCCTGAGCCG CGAGCCGGTG 
CTGCTCCTTC AGGACTCCTC TGGCGACTAC AGCCTGGCGC ATGTCCGGGA GATGGCTTGC 
TCCATCGTGG ACCAGAAGTT CCCTGAATGT GGCTTCTATG GACTCTATGA CAAGATCCTG 
CTTTTCCGTC ACGATCCTGC CTCTGAAAAC ATCCTTCAGC TGGTAAAAAT CGCAAGTGAC 
ATTCAGGAGG GCGATCTTAT TGAAGTGGTC CTGTCAGCTT CAGCCACCTT CGAAGACTTC 
CAGATCCGGC CTCACGCTCT CTTCGTTCAT TCATACAGAG CCCCTGCTTT CTGTGATCAC 
TGTGGAGAAA TGCTGTGGGG ACTGGTGCGC CAAGGCCTTA AATGTGAAGG ATGTGGCCTG 
AATTACCATA AGAGATGTGC ATTTAAAATC CCCAACAATT GCAGTGGAGT GAGAAGGAGA 
AGGCTCTCAA ATGTTTCCCT CACTGGACTG GGTACTGTCC GCACAGCGTC TGCGGAGTTC 
TCCACCAGTG CCCCCGATGA GCCTCTACTG TCTCCTGTGA GCCCTGGCTT TGAGCAAAAG 
TCTCCATCTG AGTCATTTAT CGGTCGTGAG AAGAGGTCAA ATTCTCAGTC ATATGTTGGA 
CGGCCGATTC AGCTCGACAA GCTCCTGATG TCTAAGGTGA AGGTGCCACA CACCTTTGTC 
ATCCACTCCT ACACACGGCC CACGGTCTGC CAGTTCTGTA AGAAGCTCCT CAAGGGCCTC 
TTCCGGCAGG GTTTGCAGTG CAAAGATTGC CGATTCAACT GTCACAAACG CTGTGCACCA 
AAAGTACCAA ACAACTGCTT GGGCGAAGTG ACCATCAATG GAGAATTGCT TAGCCCTGGG 
GCAGAGTCTG ATGTTGTCAT GGAAGAAGGG AGCGATGACA ATGACAGCGA ACGGAACAGT 
GGACTCATGG ATGACATGGA CGAGGCCATG GTCCAGGACA CTGAGATGGC TTTGGCGGAG 
GGTCAGAGTG ACGGTGCAGA GATGCAGGAT CCAGACGCAG ACCAGGAGGA CTCCAACAGA 
ACCATCAGCC CTTCTACGAG CAACAACATA CCGCTTATGA GGGTAGTGCA GTCTGTCAAG 
CACACAAAGA GGAGAAGCAG CACAGTGATG AAGGAAGGGT GGATGGTCCA CTACACCAGC 
AAGGACACAC TGAGGAAAAG GCATTACTGG AGACTGGACA GCAAAAGCAT CACACTCTTC 
CAAAACGACA CAGGCAGCCG GTACTACAAG GAAATTCCTT TATCAGAAAT TTTATGTCTG 
GAACCAGCAA AACCTTCAGC ATTAATTCCC ACTGGAGCTA ACCCTCATTG TTTTGAAATC 
ACTACAGCAA ACGTAGTGTA TTATGTGGGA GAAAACGTGG TCAACCCTTC AAGCCCCCCA 
CCAAACAACA GCGTCCCCCC GAGCGGCATC GGTACCGATG TGGCGAGGAT GTGGGAGGTC 
GCCATCCAGC ACGCTCTCAT GCCTGTCATC CCCAAGGGCT CCTCTGTGGG TTCCGGAACC 
AACTCACACA AAGATATTTC GGTGAGCATT TCCGTTTCGA ATAGCCAGAT TCAGGAAAAT 
GTGGATATCA GCACAGTCTA TCAGATTTTC CCGGATGAGG TTCTGGGTTC TGGACAGTTC 
GGAATTGTTT ATGGAGGTAA ACATCGTAAA ACAGGAAGAG ATGTAGCTAT TAAGATTATT 
GACAAATTAA GATTTCCAAC AAAACAAGAA AGTCAGCTTC GTAATGAGGT TGCAATTTTA 
CAGAACCTTC ATCACCCTGG TGTTGTAAAT TTGGAGTGTA TGTTTGAGAC GCCTGAAAGA 
GTGTTTGTTG TTATGGAAAA ACTCCATGGA GACATGCTGG AGATGATCCT GTCAAGTGAA 
AAGGGCAGGT TGCCAGAACA CATAACGAAG TTTTTAATTA CTCAGATACT AGTGGCTTTG 
CGACATCTTC ATTTTAAAAA CATCGTTCAC TGTGACCTCA AGCCAGAAAA TGTGTTGCTG 
GCATCAGCAG ACCCTTTCCC TCAGGTGAAA CTTTGTGATT TTGGTTTTGC CCGGATCATT 
GGCGAGAAGT CTTTCCGGAG ATCAGTGGTG GGTACCCCAG CCTACCTGGC ACCTGAGGTT 
CTGAGGAACA AGGGCTATAA CCGCTCGCTA GACATGTGGT CTGTTGGGGT CATCATCTAT 
GTGAGCCTGA GTGGCACCTT CCCTTTTAAT GAAGATGAAG ATATCCACGA TCAGATCCAG 
AACGCAGCCT TCATGTATCC ACCCAATCCC TGGAAGGAGA TTTCTCATGA AGATGACCAA 
ATGTTGAAAG TCAACTTGGG CTACATAGTA AAACTATACA ATAGTGATGA TGATGATGAT 
GATGATGATG ATGATGATGA CGACGACGAC GACGACGACG ACGACGACGA CGACGACAAC 
GACAACGACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential regulation of PKD isoforms in oxidative stress conditions through phosphorylation of a conserved Tyr in the P+1 loop.
Scientific reports7(1)887(2017 Apr)
Cobbaut M,Derua R,D?ppler H,Lou HJ,Vandoninck S,Storz P,Turk BE,Seufferlein T,Waelkens E,Janssens V,Van Lint J

Biphasic Regulation of Yes-associated Protein (YAP) Cellular Localization, Phosphorylation, and Activity by G Protein-coupled Receptor Agonists in Intestinal Epithelial Cells: A NOVEL ROLE FOR PROTEIN KINASE D (PKD).
The Journal of biological chemistry291(34)17988-8005(2016 08)
Wang J,Sinnett-Smith J,Stevens JV,Young SH,Rozengurt E

An Evolutionarily Conserved PLC-PKD-TFEB Pathway for Host Defense.
Cell reports15(8)1728-42(2016 05)
Najibi M,Labed SA,Visvikis O,Irazoqui JE

Heat shock protein 20 (HSP20) is a novel substrate for protein kinase D1 (PKD1).
Cell biochemistry and function33(7)421-6(2015 Oct)
Sin YY,Baillie GS

Protein kinase D1 mediates class IIa histone deacetylase phosphorylation and nuclear extrusion in intestinal epithelial cells: role in mitogenic signaling.
American journal of physiology. Cell physiology306(10)C961-71(2014 May)
Sinnett-Smith J,Ni Y,Wang J,Ming M,Young SH,Rozengurt E

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Prkd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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