Hcn1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hcn1
Entrez Gene ID	84390
Full Name	hyperpolarization-activated cyclic nucleotide-gated potassium channel 1
General protein information	Preferred Names hyperpolarization-activated cyclic nucleotide-gated potassium channel 1 Names potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q15
Summary	may generate neuronal pacemaker activity [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053375.1	NP_445827.1	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HCN1	NP_066550.2
Danio rerio (zebrafish)	LOC100334297	XP_005169491.1
Rattus norvegicus (Norway rat)	Hcn1	NP_445827.1
Mus musculus (house mouse)	Hcn1	NP_034538.2
Gallus gallus (chicken)	HCN1	XP_429145.3
Xenopus tropicalis (tropical clawed frog)	hcn1	XP_002933077.2
Canis lupus familiaris (dog)	HCN1	XP_855255.1
Bos taurus (cattle)	HCN1	NP_001193509.1

Gene Ontology
Bibliography

Related articles in PubMed

HCN channels segregate stimulation-evoked movement responses in neocortex and allow for coordinated forelimb movements in rodents.
Boychuk JA, Farrell JS, Palmer LA, Singleton AC, Pittman QJ, Teskey GC
The Journal of physiology595(1)247-263(2017 Jan)

Structures of the Human HCN1 Hyperpolarization-Activated Channel.
Lee CH, MacKinnon R
Cell168(1-2)111-120.e11(2017 Jan)

Essential roles of AMPA receptor GluA1 phosphorylation and presynaptic HCN channels in fast-acting antidepressant responses of ketamine.
Zhang K, Xu T, Yuan Z, Wei Z, Yamaki VN, Huang M, Huganir RL, Cai X
Science signaling9(458)ra123(2016 Dec)

Expression and cellular localization of HCN channels in rat cerebellar granule neurons.
Z??iga R, Gonz?lez D, Valenzuela C, Brown N, Z??iga L
Biochemical and biophysical research communications478(3)1429-35(2016 09)

Aberrant changes of somatostatin and neuropeptide Y in brain of a genetic rat model for epilepsy: tremor rat.
Xu X, Guo F, Cai X, Yang J, Zhao J, Min D, Wang Q, Hao L, Cai J
Acta neurobiologiae experimentalis76(3)165-75(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HCN channels play a critical role in the separation of overlapping movement responses and allow for successful reaching behaviours. These data provide a novel mechanism for the encoding of multiple movement responses within shared networks of motor cortex. This mechanism supports a viewpoint of primary motor cortex as a site of dynamic integration for behavioural output
Title: HCN channels segregate stimulation-evoked movement responses in neocortex and allow for coordinated forelimb movements in rodents.

results implicate presynaptic NMDA receptor inhibition followed by reduced activity of presynaptic HCN1 channels, which would result in an increase in glutamate release and postsynaptic glutamate receptor activity, as a mechanism of ketamine action.
Title: Essential roles of AMPA receptor GluA1 phosphorylation and presynaptic HCN channels in fast-acting antidepressant responses of ketamine.

the expression and relative abundances of HCN channels in cerebellum and primary cultures of cerebellar granule neurons, is described.
Title: Expression and cellular localization of HCN channels in rat cerebellar granule neurons.

Hyperpolarization-activated cyclic nucleotide-gated (HCN) 1 protein expression levels were increased in the thalamus under chronic pain condition. Results suggest, increased HCN channel activity in the thalamus of the ascending nociceptive pathway contributes to both chronic neuropathic and inflammatory pain conditions.
Title: Inhibition of HCN channel activity in the thalamus attenuates chronic pain in rats.

This study demonstrated that the aberrant changes of SST and NPY in several TRM brain regions(hcn1 mutation), suggesting that the peptidergic system might be involved in TRM epileptiform activity.
Title: Aberrant changes of somatostatin and neuropeptide Y in brain of a genetic rat model for epilepsy: tremor rat.

The following Hcn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hcn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13109	NM_053375.1 Latest version!	Rattus norvegicus hyperpolarization-activated cyclic nucleotide-gated potassium channel 1 (Hcn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hcn1 ( NM_053375.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445827.1 Rattus norvegicus hyperpolarization-activated cyclic nucleotide-gated potassium channel 1 (Hcn1), mRNA.

Price & Availability↑

CloneID	ORa13109
Clone ID Related Accession (Same CDS sequence)	NM_053375.1
Accession Version	NM_053375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Rattus norvegicus(Norway rat)
Product	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF247450.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF247450.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGGCG GCGGCAAGCC CAACTCCGCT TCCAACAGCC GCGACGATGG CAACAGCGTC 
TACCCCTCCA AGGCGCCCGC GACGGGGCCG GCGGCGGCCG ACAAGCGCCT GGGGACCCCG 
CCGGGGGGCG GCGCGGCCGG GAAGGAACAC GGCAACTCCG TGTGCTTCAA GGTGGACGGC 
GGCGGAGGAG AGGAGCCGGC GGGCAGCTTC GAGGATGCCG AGGGGCCCCG GCGACAGTAC 
GGTTTCATGC AGAGGCAGTT CACCTCCATG CTGCAGCCTG GGGTCAACAA ATTCTCCCTC 
CGCATGTTCG GGAGCCAGAA GGCGGTGGAG AAGGAGCAGG AAAGGGTTAA AACTGCAGGC 
TTCTGGATTA TCCATCCGTA CAGTGACTTC AGGTTTTATT GGGATTTAAT AATGCTTATA 
ATGATGGTTG GAAATTTGGT CATCATACCA GTTGGAATCA CATTCTTCAC AGAGCAAACA 
ACAACACCGT GGATTATTTT CAATGTGGCA TCAGATACAG TTTTCCTGTT GGACCTAATC 
ATGAATTTTA GGACTGGGAC TGTCAACGAA GACAGCTCTG AAATCATCCT GGACCCTAAA 
GTAATCAAGA TGAATTATTT AAAAAGCTGG TTCGTGGTGG ACTTCATCTC CTCGATCCCG 
GTGGATTATA TCTTTCTTAT TGTAGAGAAA GGAATGGATT CGGAAGTTTA CAAGACCGCC 
AGAGCACTTC GGATCGTGAG GTTTACAAAA ATTCTCAGTC TCTTGCGTTT ATTACGCCTT 
TCAAGGTTAA TTAGATACAT ACACCAGTGG GAAGAGATAT TCCACATGAC ATATGATCTC 
GCCAGTGCAG TGGTGAGAAT CTTCAACCTC ATTGGCATGA TGCTGCTCCT GTGTCACTGG 
GATGGCTGTC TTCAGTTTCT GGTCCCCCTG CTGCAGGACT TCCCACCGGA TTGCTGGGTT 
TCTCTAAATG AAATGGTTAA TGATTCATGG GGGAAACAGT ATTCCTACGC ACTCTTCAAA 
GCTATGAGTC ACATGCTGTG CATTGGTTAT GGCGCCCAGG CCCCCGTCAG CATGTCTGAC 
CTCTGGATTA CCATGCTGAG CATGATTGTT GGGGCCACCT GCTATGCCAT GTTTGTCGGC 
CATGCCACAG CTTTGATCCA GTCTCTGGAT TCTTCAAGGA GGCAGTATCA AGAGAAGTAC 
AAGCAAGTAG AGCAATACAT GTCATTCCAC AAGTTACCAG CTGACATGCG CCAGAAGATA 
CATGATTACT ATGAGCACCG ATACCAAGGC AAGATCTTCG ATGAGGAAAA TATTCTCAGT 
GAACTTAATG ATCCTCTGAG AGAGGAAATA GTCAACTTCA ACTGCCGGAA ACTGGTGGCC 
ACCATGCCTC TCTTTGCTAA CGCGGATCCC AATTTCGTGA CGGCCATGCT GAGCAAGCTG 
AGATTTGAGG TGTTCCAGCC CGGAGACTAT ATCATTCGAG AAGGAGCTGT GGGGAAGAAA 
ATGTATTTCA TCCAGCATGG TGTGGCTGGT GTCATCACCA AGTCCAGTAA AGAAATGAAG 
TTGACAGACG GCTCTTACTT TGGAGAAATA TGCCTGCTGA CCAAGGGCCG GCGCACTGCC 
AGTGTTCGAG CTGATACATA CTGTCGCCTT TACTCCCTTT CGGTGGACAA TTTCAACGAG 
GTCTTGGAGG AATATCCAAT GATGAGAAGA GCCTTTGAGA CAGTTGCTAT TGACCGACTA 
GATCGGATAG GCAAGAAAAA CTCTATTCTC CTGCAGAAGT TCCAGAAGGA TCTGAACACT 
GGTGTTTTCA ACAACCAGGA GAATGAGATC CTGAAGCAGA TTGTGAAGCA TGACAGAGAG 
ATGGTACAAG CGATCCCTCC AATCAACTAT CCTCAAATGA CAGCCCTGAA TTGCACATCT 
TCAACCACCA CCCCAACGTC GCGCATGAGG ACCCAATCTC CACCAGTCTA CACAGCGACC 
AGCCTCTCTC ACAGCAACCT GCACTCACCC AGCCCCAGCA CACAGACGCC TCAACCCTCA 
GCCATCCTTT CACCCTGCTC CTACACCACA GCAGTCTGCA GTCCTCCTAT ACAGAGCCCC 
CTGGCCACGC GAACTTTCCA TTATGCCTCT CCCACTGCAT CCCAATTGTC ACTCATGCAG 
CAGCCTCAGC CGCAGCTACA GCAATCCCAG GTACAGCAGA CTCAGACTCA GACTCAGCAG 
CAGCAGCAGC AACAGCAGCC GCAGCCGCAG CCGCAGCAGC CGCAACAGCA ACAACAGCAG 
CAACAGCAGC AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACAGCCACA GACACCTGGT 
AGTTCCACAC CGAAAAATGA AGTGCACAAG AGCACTCAAG CTCTTCATAA CACCCACCTG 
ACCAGAGAAG TCAGGCCCCT CTCTGCCTCG CAGCCTTCGC TGCCCCATGA GGTCTCCACT 
ATGATCTCCA GACCGCATCC CACTGTGGGC GAGTCCCTGG CTTCCATCCC TCAACCCGTG 
GCAACAGTCC ACAGCACTGG CCTTCAGGCA GGGAGCAGGA GCACCGTGCC ACAGCGTGTC 
ACCTTGTTCA GACAGATGTC CTCGGGAGCC ATTCCCCCCA ACCGAGGAGT GCCTCCAGCA 
CCCCCGCCAC CAGCAGCTGT GCAGAGAGAG TCTCCCTCAG TCTTAAATAA AGACCCAGAT 
GCAGAAAAAC CCCGTTTTGC TTCGAATTTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445827.1
CDS	75..2807
Translation	MEGGGKPNSASNSRDDGNSVYPSKAPATGPAAADKRLGTPPGGGAAGKEHGNSVCFKVDGGGGEEPAGSFEDAEGPRRQYGFMQRQFTSMLQPGVNKFSLRMFGSQKAVEKEQERVKTAGFWIIHPYSDFRFYWDLIMLIMMVGNLVIIPVGITFFTEQTTTPWIIFNVASDTVFLLDLIMNFRTGTVNEDSSEIILDPKVIKMNYLKSWFVVDFISSIPVDYIFLIVEKGMDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIFNLIGMMLLLCHWDGCLQFLVPLLQDFPPDCWVSLNEMVNDSWGKQYSYALFKAMSHMLCIGYGAQAPVSMSDLWITMLSMIVGATCYAMFVGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADMRQKIHDYYEHRYQGKIFDEENILSELNDPLREEIVNFNCRKLVATMPLFANADPNFVTAMLSKLRFEVFQPGDYIIREGAVGKKMYFIQHGVAGVITKSSKEMKLTDGSYFGEICLLTKGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLQKFQKDLNTGVFNNQENEILKQIVKHDREMVQAIPPINYPQMTALNCTSSTTTPTSRMRTQSPPVYTATSLSHSNLHSPSPSTQTPQPSAILSPCSYTTAVCSPPIQSPLATRTFHYASPTASQLSLMQQPQPQLQQSQVQQTQTQTQQQQQQQQPQPQPQQPQQQQQQQQQQQQQQQQQQQQQPQTPGSSTPKNEVHKSTQALHNTHLTREVRPLSASQPSLPHEVSTMISRPHPTVGESLASIPQPVATVHSTGLQAGSRSTVPQRVTLFRQMSSGAIPPNRGVPPAPPPPAAVQRESPSVLNKDPDAEKPRFASNL

Target ORF information:

RefSeq Version	NM_053375.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hyperpolarization-activated cyclic nucleotide-gated potassium channel 1 (Hcn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053375.1

ORF Insert Sequence:

ATGGAAGGCG GCGGCAAGCC CAACTCCGCT TCCAACAGCC GCGACGATGG CAACAGCGTC 
TACCCCTCCA AGGCGCCCGC GACGGGGCCG GCGGCGGCCG ACAAGCGCCT GGGGACCCCG 
CCGGGGGGCG GCGCGGCCGG GAAGGAACAC GGCAACTCCG TGTGCTTCAA GGTGGACGGC 
GGCGGAGGAG AGGAGCCGGC GGGCAGCTTC GAGGATGCCG AGGGGCCCCG GCGACAGTAC 
GGTTTCATGC AGAGGCAGTT CACCTCCATG CTGCAGCCTG GGGTCAACAA ATTCTCCCTC 
CGCATGTTCG GGAGCCAGAA GGCGGTGGAG AAGGAGCAGG AAAGGGTTAA AACTGCAGGC 
TTCTGGATTA TCCATCCGTA CAGTGACTTC AGGTTTTATT GGGATTTAAT AATGCTTATA 
ATGATGGTTG GAAATTTGGT CATCATACCA GTTGGAATCA CATTCTTCAC AGAGCAAACA 
ACAACACCGT GGATTATTTT CAATGTGGCA TCAGATACAG TTTTCCTGTT GGACCTAATC 
ATGAATTTTA GGACTGGGAC TGTCAACGAA GACAGCTCTG AAATCATCCT GGACCCTAAA 
GTAATCAAGA TGAATTATTT AAAAAGCTGG TTCGTGGTGG ACTTCATCTC CTCGATCCCG 
GTGGATTATA TCTTTCTTAT TGTAGAGAAA GGAATGGATT CGGAAGTTTA CAAGACCGCC 
AGAGCACTTC GGATCGTGAG GTTTACAAAA ATTCTCAGTC TCTTGCGTTT ATTACGCCTT 
TCAAGGTTAA TTAGATACAT ACACCAGTGG GAAGAGATAT TCCACATGAC ATATGATCTC 
GCCAGTGCAG TGGTGAGAAT CTTCAACCTC ATTGGCATGA TGCTGCTCCT GTGTCACTGG 
GATGGCTGTC TTCAGTTTCT GGTCCCCCTG CTGCAGGACT TCCCACCGGA TTGCTGGGTT 
TCTCTAAATG AAATGGTTAA TGATTCATGG GGGAAACAGT ATTCCTACGC ACTCTTCAAA 
GCTATGAGTC ACATGCTGTG CATTGGTTAT GGCGCCCAGG CCCCCGTCAG CATGTCTGAC 
CTCTGGATTA CCATGCTGAG CATGATTGTT GGGGCCACCT GCTATGCCAT GTTTGTCGGC 
CATGCCACAG CTTTGATCCA GTCTCTGGAT TCTTCAAGGA GGCAGTATCA AGAGAAGTAC 
AAGCAAGTAG AGCAATACAT GTCATTCCAC AAGTTACCAG CTGACATGCG CCAGAAGATA 
CATGATTACT ATGAGCACCG ATACCAAGGC AAGATCTTCG ATGAGGAAAA TATTCTCAGT 
GAACTTAATG ATCCTCTGAG AGAGGAAATA GTCAACTTCA ACTGCCGGAA ACTGGTGGCC 
ACCATGCCTC TCTTTGCTAA CGCGGATCCC AATTTCGTGA CGGCCATGCT GAGCAAGCTG 
AGATTTGAGG TGTTCCAGCC CGGAGACTAT ATCATTCGAG AAGGAGCTGT GGGGAAGAAA 
ATGTATTTCA TCCAGCATGG TGTGGCTGGT GTCATCACCA AGTCCAGTAA AGAAATGAAG 
TTGACAGACG GCTCTTACTT TGGAGAAATA TGCCTGCTGA CCAAGGGCCG GCGCACTGCC 
AGTGTTCGAG CTGATACATA CTGTCGCCTT TACTCCCTTT CGGTGGACAA TTTCAACGAG 
GTCTTGGAGG AATATCCAAT GATGAGAAGA GCCTTTGAGA CAGTTGCTAT TGACCGACTA 
GATCGGATAG GCAAGAAAAA CTCTATTCTC CTGCAGAAGT TCCAGAAGGA TCTGAACACT 
GGTGTTTTCA ACAACCAGGA GAATGAGATC CTGAAGCAGA TTGTGAAGCA TGACAGAGAG 
ATGGTACAAG CGATCCCTCC AATCAACTAT CCTCAAATGA CAGCCCTGAA TTGCACATCT 
TCAACCACCA CCCCAACGTC GCGCATGAGG ACCCAATCTC CACCAGTCTA CACAGCGACC 
AGCCTCTCTC ACAGCAACCT GCACTCACCC AGCCCCAGCA CACAGACGCC TCAACCCTCA 
GCCATCCTTT CACCCTGCTC CTACACCACA GCAGTCTGCA GTCCTCCTAT ACAGAGCCCC 
CTGGCCACGC GAACTTTCCA TTATGCCTCT CCCACTGCAT CCCAATTGTC ACTCATGCAG 
CAGCCTCAGC CGCAGCTACA GCAATCCCAG GTACAGCAGA CTCAGACTCA GACTCAGCAG 
CAGCAGCAGC AACAGCAGCC GCAGCCGCAG CCGCAGCAGC CGCAACAGCA ACAACAGCAG 
CAACAGCAGC AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACAGCCACA GACACCTGGT 
AGTTCCACAC CGAAAAATGA AGTGCACAAG AGCACTCAAG CTCTTCATAA CACCCACCTG 
ACCAGAGAAG TCAGGCCCCT CTCTGCCTCG CAGCCTTCGC TGCCCCATGA GGTCTCCACT 
ATGATCTCCA GACCGCATCC CACTGTGGGC GAGTCCCTGG CTTCCATCCC TCAACCCGTG 
GCAACAGTCC ACAGCACTGG CCTTCAGGCA GGGAGCAGGA GCACCGTGCC ACAGCGTGTC 
ACCTTGTTCA GACAGATGTC CTCGGGAGCC ATTCCCCCCA ACCGAGGAGT GCCTCCAGCA 
CCCCCGCCAC CAGCAGCTGT GCAGAGAGAG TCTCCCTCAG TCTTAAATAA AGACCCAGAT 
GCAGAAAAAC CCCGTTTTGC TTCGAATTTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HCN channels segregate stimulation-evoked movement responses in neocortex and allow for coordinated forelimb movements in rodents.
The Journal of physiology595(1)247-263(2017 Jan)
Boychuk JA,Farrell JS,Palmer LA,Singleton AC,Pittman QJ,Teskey GC

Structures of the Human HCN1 Hyperpolarization-Activated Channel.
Cell168(1-2)111-120.e11(2017 Jan)
Lee CH,MacKinnon R

Essential roles of AMPA receptor GluA1 phosphorylation and presynaptic HCN channels in fast-acting antidepressant responses of ketamine.
Science signaling9(458)ra123(2016 Dec)
Zhang K,Xu T,Yuan Z,Wei Z,Yamaki VN,Huang M,Huganir RL,Cai X

Expression and cellular localization of HCN channels in rat cerebellar granule neurons.
Biochemical and biophysical research communications478(3)1429-35(2016 09)
Z??iga R,Gonz?lez D,Valenzuela C,Brown N,Z??iga L

Aberrant changes of somatostatin and neuropeptide Y in brain of a genetic rat model for epilepsy: tremor rat.
Acta neurobiologiae experimentalis76(3)165-75(2016)
Xu X,Guo F,Cai X,Yang J,Zhao J,Min D,Wang Q,Hao L,Cai J

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Hcn1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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