hcn1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	hcn1
Entrez Gene ID	100490760
Full Name	hyperpolarization activated cyclic nucleotide gated potassium channel 1
General protein information	Preferred Names hyperpolarization activated cyclic nucleotide gated potassium channel 1 Names LOW QUALITY PROTEIN: potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031894296.1	XP_031750156.1	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
XM_002933031.4	XP_002933077.2	LOW QUALITY PROTEIN: potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HCN1	NP_066550.2
Danio rerio (zebrafish)	LOC100334297	XP_005169491.1
Rattus norvegicus (Norway rat)	Hcn1	NP_445827.1
Mus musculus (house mouse)	Hcn1	NP_034538.2
Gallus gallus (chicken)	HCN1	XP_429145.3
Xenopus tropicalis (tropical clawed frog)	hcn1	XP_002933077.2
Canis lupus familiaris (dog)	HCN1	XP_855255.1
Bos taurus (cattle)	HCN1	NP_001193509.1

Gene Ontology

The following hcn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hcn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa03399	XM_002933031.4 Latest version!	Xenopus tropicalis hyperpolarization activated cyclic nucleotide gated potassium channel 1 (hcn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa49182	XM_031894296.1 Latest version!	Xenopus tropicalis hyperpolarization activated cyclic nucleotide gated potassium channel 1 (hcn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hcn1 ( XM_002933031.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933077.2 Xenopus tropicalis hyperpolarization activated cyclic nucleotide gated potassium channel 1 (hcn1), mRNA.

Price & Availability↑

CloneID	OXa03399
Clone ID Related Accession (Same CDS sequence)	XM_002933031.4
Accession Version	XM_002933031.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LOW QUALITY PROTEIN: potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002933031.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGAAAGCA AATTCAACTC CTCCACCAAC AGCAGCAGGG ACGACGGCAA TAATGTGTTG 
CAGGGCAAAG CGGAGAAAAG TCTGACTGCC AGCACTTCCA GCGTCAAGGA GCATGGCAAC 
TCCGTCTGCT TCAAAACGGA CGCCGCCGAC GATCCCGTAG TTGGATTTGA GGACGCGGAA 
GGGCCCAGCC GGCCTCACGG CTTCATGCAG CGACAGTTCG GCTCCTTGAT GCAGCCGGGA 
GTCAATAAAT TCTCCCTCCG CATGTTTGGC AGTCAGAAGG CGGTGGAGAA GGAACAGGAG 
AGGGTTAAAA CGGCAGGAGT GTGGATCATC CATCCCTACA GTGATTTCAG ATTTTACTGG 
GACCTGATCA TGCTCATCAT GATGGTCGGA AATCTTGTCA TAATACCAGT CGGAATCACG 
TTCTTCACAG AGCAAACAAC AACGCCGTGG ATTATCTTCA ATGTGGCCTC CGACACCGTC 
TTTTTATTTG ACTTGTTCAT GAATTTTAGA ACTGGAATTG TTATTGAGGA CAATTCAGAG 
ATAATCCTCG ATCCAAAAGT CATAAAAATG AATTATCTCA AAAGCTGGTT CGTAGTGGAC 
TTCATCTCCT CTATTCCAGT GGATTATATC TTTCTCATTG TCGAGAAAGG CATGGATTCG 
GAAGTGTACA AGACCGCTCG GGCGCTCCGC ATAGTGAGGT TTACAAAAAT TCTCAGTTTG 
CTGCGATTGC TGCGACTTTC GAGGTTAATT AGATATATTC ATCAGTGGGA AGAGATTTTC 
CATATGACTT ATGACTTGGC TAGTGCCGTG GTGAGAATCT TCAATCTCAT TGGCATGATG 
CTGCTGCTTT GTCACTGGGA TGGCTGCCTT CAGTTTTTGG TACCATTACT TCAGGAATTT 
CCCCCAGACT GCTGGGTATC ATTAAATAAA CATGTGAATG ATTCCTGGGG AAAACAGTAT 
TCCTATGCTC TCTTTAAAGC AATGAGCCAT ATGCTGTGCA TTGGATATGG GGCCCGTGCA 
CCTGTCAGCA TGTCCGATCT GTGGATCACT ATGTTGAGCA TGATTGTGGG AGCCACGTGC 
TATGCCATGT TTGTTGGACA TGCCACAGCC TTAATACAGT CCCTGGATTC TTCACGTCGC 
CAGTACCAAG AAAAGTACAA ACAAGTGGAA CAGTACATGT CATTTCACAA ATTGCCTTCT 
GATATGCGGC AGAAGATACA CGATTATTAT GAGCACCGAT ATCAAGGAAA AATATTCGAT 
GAAGACAACA TTCTTAATGA GCTAAATGAC CCATTGAGAG AGGAAATAGT CAACTTTAAC 
TGTCGGAAAC TGGTGGCTAC GATGCCTCTG TTTGCTAACG CGGATCCAAA CTTTGTCACA 
GCCATGCTGA GCAAACTGAA GTTTGAAGTG TTTCAGCCTG GAGATTACAT TATTCGTGAA 
GGAGCTGTGG GGAAGAAGAT GTATTTTATA CAGCATGGTG TGGCAGGTGT CATTACTAAA 
TCCAGCAAAG AAATGAAGTT AACTGATGGA TCTTATTTTG GAGAGATTTG CCTTTTGACA 
AAAGGTCGGA GAACTGCTAG CGTCCGAGCA GAAACCTATT GCCGCCTGTA TTCTCTTTCG 
GTGGAGAACT TCAATGAAGT GCTGGAAGAA TATCCTATGA TGAGACGAGC CTTTGAAACT 
GTGGCCATAG ACAGATTAGA CAGGATAGGG AAGAAGAACT CAATTCTTCA GCAACGATTT 
CAAAAGGACC TCAACACGGG AATATTCAAC AACCAGGAGA GCGAAATCCT GAAGCAGATG 
CTGACACAAG ACAGGGAGAT GGTGGAAGGT CATCCCTCAG TTAGCTACCA GCAGCTGCCA 
GCTCTAAATT CTAGCACAAG TTCATCATTG AGAATGAGGA CCCGATCTCC ACCTGTTTAT 
AATGCAAACA GTTTGTCTCA TGGAAACCTC CAATCACCAA CACCCAGCCC CCAAACGCCT 
CAACAAGCTG TTGTGTTTTC ACCTTCTTCC TTTACTTCTG CAGCCTGCAG TCCTTCAGTT 
CAGAGTCCAT TGGCTGGTCG AACTTTTCAG TATAGTTCAC TTACAGCATC ACAGCTTTCA 
TTAATTCAGC AACAGCAGCA AGCATCTGGC GCATCCCAGA TACATGAAGT TCATAAAAGC 
GCTCAAGCTC TTGCAAACAC TAACCTAACT CGAGAAGTGA GGCCTCTTTC AGCCTCACAG 
CCTTCTTTGC CACATGAGAT ATCTACTTTA GGATCTAAAC CACACCCGAC TGTGGGAGAA 
TCGTTAGCTT CACTCCCCCA GCCAATATCA AATGTTCAGG CTGCAGGTAC TCAGTCTGGA 
AGTCGGAGCA ATATCCCTCC AAGGGTAGCC CTTTTTCGGC AGATGTCTTC AGGAGCTTTA 
CCTCCCGCAA GAGTCGGAGC AACAACACAG CCCTCTCCTG CGCCCCCCAC CAAGGATTCC 
TCAACAGTAT CCAGCACAGA CACGGACACA GACAAACTAA AATATGTTTC AAATTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933077.2
CDS	173..2692
Translation	MESKFNSSTNSSRDDGNNVLQGKAEKSLTASTSSVKEHGNSVCFKTDAADDPVVGFEDAEGPSRPHGFMQRQFGSLMQPGVNKFSLRMFGSQKAVEKEQERVKTAGVWIIHPYSDFRFYWDLIMLIMMVGNLVIIPVGITFFTEQTTTPWIIFNVASDTVFLFDLFMNFRTGIVIEDNSEIILDPKVIKMNYLKSWFVVDFISSIPVDYIFLIVEKGMDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIFNLIGMMLLLCHWDGCLQFLVPLLQEFPPDCWVSLNKHVNDSWGKQYSYALFKAMSHMLCIGYGARAPVSMSDLWITMLSMIVGATCYAMFVGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPSDMRQKIHDYYEHRYQGKIFDEDNILNELNDPLREEIVNFNCRKLVATMPLFANADPNFVTAMLSKLKFEVFQPGDYIIREGAVGKKMYFIQHGVAGVITKSSKEMKLTDGSYFGEICLLTKGRRTASVRAETYCRLYSLSVENFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILQQRFQKDLNTGIFNNQESEILKQMLTQDREMVEGHPSVSYQQLPALNSSTSSSLRMRTRSPPVYNANSLSHGNLQSPTPSPQTPQQAVVFSPSSFTSAACSPSVQSPLAGRTFQYSSLTASQLSLIQQQQQASGASQIHEVHKSAQALANTNLTREVRPLSASQPSLPHEISTLGSKPHPTVGESLASLPQPISNVQAAGTQSGSRSNIPPRVALFRQMSSGALPPARVGATTQPSPAPPTKDSSTVSSTDTDTDKLKYVSNL

Target ORF information:

RefSeq Version	XM_002933031.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis hyperpolarization activated cyclic nucleotide gated potassium channel 1 (hcn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933031.4

ORF Insert Sequence:

ATGGAAAGCA AATTCAACTC CTCCACCAAC AGCAGCAGGG ACGACGGCAA TAATGTGTTG 
CAGGGCAAAG CGGAGAAAAG TCTGACTGCC AGCACTTCCA GCGTCAAGGA GCATGGCAAC 
TCCGTCTGCT TCAAAACGGA CGCCGCCGAC GATCCCGTAG TTGGATTTGA GGACGCGGAA 
GGGCCCAGCC GGCCTCACGG CTTCATGCAG CGACAGTTCG GCTCCTTGAT GCAGCCGGGA 
GTCAATAAAT TCTCCCTCCG CATGTTTGGC AGTCAGAAGG CGGTGGAGAA GGAACAGGAG 
AGGGTTAAAA CGGCAGGAGT GTGGATCATC CATCCCTACA GTGATTTCAG ATTTTACTGG 
GACCTGATCA TGCTCATCAT GATGGTCGGA AATCTTGTCA TAATACCAGT CGGAATCACG 
TTCTTCACAG AGCAAACAAC AACGCCGTGG ATTATCTTCA ATGTGGCCTC CGACACCGTC 
TTTTTATTTG ACTTGTTCAT GAATTTTAGA ACTGGAATTG TTATTGAGGA CAATTCAGAG 
ATAATCCTCG ATCCAAAAGT CATAAAAATG AATTATCTCA AAAGCTGGTT CGTAGTGGAC 
TTCATCTCCT CTATTCCAGT GGATTATATC TTTCTCATTG TCGAGAAAGG CATGGATTCG 
GAAGTGTACA AGACCGCTCG GGCGCTCCGC ATAGTGAGGT TTACAAAAAT TCTCAGTTTG 
CTGCGATTGC TGCGACTTTC GAGGTTAATT AGATATATTC ATCAGTGGGA AGAGATTTTC 
CATATGACTT ATGACTTGGC TAGTGCCGTG GTGAGAATCT TCAATCTCAT TGGCATGATG 
CTGCTGCTTT GTCACTGGGA TGGCTGCCTT CAGTTTTTGG TACCATTACT TCAGGAATTT 
CCCCCAGACT GCTGGGTATC ATTAAATAAA CATGTGAATG ATTCCTGGGG AAAACAGTAT 
TCCTATGCTC TCTTTAAAGC AATGAGCCAT ATGCTGTGCA TTGGATATGG GGCCCGTGCA 
CCTGTCAGCA TGTCCGATCT GTGGATCACT ATGTTGAGCA TGATTGTGGG AGCCACGTGC 
TATGCCATGT TTGTTGGACA TGCCACAGCC TTAATACAGT CCCTGGATTC TTCACGTCGC 
CAGTACCAAG AAAAGTACAA ACAAGTGGAA CAGTACATGT CATTTCACAA ATTGCCTTCT 
GATATGCGGC AGAAGATACA CGATTATTAT GAGCACCGAT ATCAAGGAAA AATATTCGAT 
GAAGACAACA TTCTTAATGA GCTAAATGAC CCATTGAGAG AGGAAATAGT CAACTTTAAC 
TGTCGGAAAC TGGTGGCTAC GATGCCTCTG TTTGCTAACG CGGATCCAAA CTTTGTCACA 
GCCATGCTGA GCAAACTGAA GTTTGAAGTG TTTCAGCCTG GAGATTACAT TATTCGTGAA 
GGAGCTGTGG GGAAGAAGAT GTATTTTATA CAGCATGGTG TGGCAGGTGT CATTACTAAA 
TCCAGCAAAG AAATGAAGTT AACTGATGGA TCTTATTTTG GAGAGATTTG CCTTTTGACA 
AAAGGTCGGA GAACTGCTAG CGTCCGAGCA GAAACCTATT GCCGCCTGTA TTCTCTTTCG 
GTGGAGAACT TCAATGAAGT GCTGGAAGAA TATCCTATGA TGAGACGAGC CTTTGAAACT 
GTGGCCATAG ACAGATTAGA CAGGATAGGG AAGAAGAACT CAATTCTTCA GCAACGATTT 
CAAAAGGACC TCAACACGGG AATATTCAAC AACCAGGAGA GCGAAATCCT GAAGCAGATG 
CTGACACAAG ACAGGGAGAT GGTGGAAGGT CATCCCTCAG TTAGCTACCA GCAGCTGCCA 
GCTCTAAATT CTAGCACAAG TTCATCATTG AGAATGAGGA CCCGATCTCC ACCTGTTTAT 
AATGCAAACA GTTTGTCTCA TGGAAACCTC CAATCACCAA CACCCAGCCC CCAAACGCCT 
CAACAAGCTG TTGTGTTTTC ACCTTCTTCC TTTACTTCTG CAGCCTGCAG TCCTTCAGTT 
CAGAGTCCAT TGGCTGGTCG AACTTTTCAG TATAGTTCAC TTACAGCATC ACAGCTTTCA 
TTAATTCAGC AACAGCAGCA AGCATCTGGC GCATCCCAGA TACATGAAGT TCATAAAAGC 
GCTCAAGCTC TTGCAAACAC TAACCTAACT CGAGAAGTGA GGCCTCTTTC AGCCTCACAG 
CCTTCTTTGC CACATGAGAT ATCTACTTTA GGATCTAAAC CACACCCGAC TGTGGGAGAA 
TCGTTAGCTT CACTCCCCCA GCCAATATCA AATGTTCAGG CTGCAGGTAC TCAGTCTGGA 
AGTCGGAGCA ATATCCCTCC AAGGGTAGCC CTTTTTCGGC AGATGTCTTC AGGAGCTTTA 
CCTCCCGCAA GAGTCGGAGC AACAACACAG CCCTCTCCTG CGCCCCCCAC CAAGGATTCC 
TCAACAGTAT CCAGCACAGA CACGGACACA GACAAACTAA AATATGTTTC AAATTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hcn1 ( XM_031894296.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750156.1 Xenopus tropicalis hyperpolarization activated cyclic nucleotide gated potassium channel 1 (hcn1), mRNA.

Price & Availability↑

CloneID	OXa49182
Clone ID Related Accession (Same CDS sequence)	XM_031894296.1
Accession Version	XM_031894296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCA AATTCAACTC CTCCACCAAC AGCAGCAGGG ACGACGGCAA TAATGTGTTG 
CAGGGCAAAG CGGAGAAAAG TCTGACTGCC AGCACTTCCA GCGTCAAGGA GCATGGCAAC 
TCCGTCTGCT TCAAAACGGA CGCCGCCGAC GATCCCGTAG TTGGATTTGA GGACGCGGAA 
GGGCCCAGCC GGCCTCACGG CTTCATGCAG CGACAGTTCG GCTCCTTGAT GCAGCCGGGA 
GTCAATAAAT TCTCCCTCCG CATGTTTGGC AGTCAGAAGG CGGTGGAGAA GGAGCAGGAG 
AGGGTTAAAA CGGCAGGAGT GTGGATCATC CATCCCTACA GTGATTTCAG ATTTTACTGG 
GACCTGATCA TGCTCATCAT GATGGTCGGA AATCTTGTCA TAATACCAGT CGGAATCACG 
TTCTTCACAG AGCAAACAAC AACGCCGTGG ATTATCTTCA ATGTGGCCTC CGACACCGTC 
TTTTTATTTG ACTTGTTCAT GAATTTTAGA ACTGGAATTG TTATTGAGGA CAATTCAGAG 
ATAATCCTCG ATCCAAAAGT CATAAAAATG AATTATCTCA AAAGCTGGTT CGTAGTGGAC 
TTCATCTCCT CTATTCCAGT GGATTATATC TTTCTCATTG TCGAGAAAGG CATGGATTCG 
GAAGTGTACA AGACCGCTCG GGCGCTCCGC ATAGTGAGGT TTACAAAAAT TCTCAGTTTG 
CTGCGATTGC TGCGACTTTC GAGGTTAATT AGATATATTC ATCAGTGGGA AGAGATTTTC 
CATATGACTT ATGACTTGGC TAGTGCCGTG GTGAGAATCT TCAATCTCAT TGGCATGATG 
CTGCTGCTTT GTCACTGGGA TGGCTGCCTT CAGTTTTTGG TACCATTACT TCAGGAATTT 
CCACCAGACT GCTGGGTATC ATTAAATAAC ATGGTGAATG ATTCCTGGGG AAAACAGTAT 
TCCTATGCTC TCTTTAAAGC AATGAGCCAT ATGCTGTGCA TTGGATATGG GGCCCGTGCA 
CCTGTCAGCA TGTCCGATCT GTGGATCACT ATGTTGAGCA TGATTGTGGG AGCCACGTGC 
TATGCCATGT TTGTTGGACA TGCCACAGCC TTAATACAGT CCCTGGATTC TTCACGTCGC 
CAGTACCAAG AAAAGTACAA ACAAGTGGAA CAGTACATGT CATTTCACAA ATTGCCTTCT 
GATATGCGGC AGAAGATACA CGATTATTAT GAGCACCGAT ATCAAGGAAA AATATTCGAT 
GAAGACAACA TTCTTAATGA GCTAAATGAC CCATTGAGAG AGGAAATAGT CAACTTTAAC 
TGTCGGAAAC TGGTGGCTAC GATGCCTCTG TTTGCTAACG CGGATCCAAA CTTTGTCACA 
GCCATGCTGA GCAAACTGAA GTTTGAAGTG TTTCAGCCTG GAGATTACAT TATTCGTGAA 
GGAGCTGTGG GGAAGAAGAT GTATTTTATA CAGCATGGTG TGGCAGGTGT CATTACTAAA 
TCCAGCAAAG AAATGAAGTT AACTGATGGA TCTTATTTTG GAGAGATTTG CCTTTTGACA 
AAAGGTCGGA GAACTGCTAG CGTCCGAGCA GAAACCTATT GCCGCCTGTA TTCTCTTTCG 
GTGGAGAACT TCAATGAAGT GCTGGAAGAA TATCCTATGA TGAGACGAGC CTTTGAAACT 
GTGGCCATAG ACAGATTAGA CAGGATAGGG AAGAAGAACT CAATTCTTCA GCAACGATTT 
CAAAAGGACC TCAACACGGG AATATTCAAC AACCAGGAGA GCGAAATCCT GAAGCAGATG 
CTGACACAAG ACAGGGAGAT GGTGGAAGGT CATCCCTCAG TTAGCTACCA GCAGCTGCCA 
GCTCTAAATT CTAGCACAAG TTCATCATTG AGAATGAGGA CCCGATCTCC ACCTGTTTAT 
AATGCAAACA GTTTGTCTCA TGGAAACCTC CAATCACCAA CACCCAGCCC CCAGACGCCT 
CAACAAGCTG TTGTGTTTTC ACCTTCTTCC TTTACTTCTG CAGCCTGCAG TCCTTCAGTT 
CAGAGTCCAT TGGCTGGTCG AACTTTTCAG TATAGTTCAC TTACAGCATC ACAGCTTTCA 
TTAATTCAGC AACAGCAGCA AGCATCTGGC GCATCCCAGA TACATGAAGT TCATAAAAGC 
GCTCAAGCTC TTGCAAACAC TAACCTAACT CGAGAAGTGA GGCCTCTTTC AGCCTCACAG 
CCTTCTTTGC CACATGAGAT ATCTACTTTA GGATCTAAAC CACACCCGAC TGTGGGAGAA 
TCGTTAGCTT CACTCCCCCA GCCAATATCA AATGTTCAGG CTGCAGGTAC TCAGTCTGGA 
AGTCGGAGCA ATATCCCTCC AAGGGTAGCC CTTTTTCGGC AGATGTCTTC AGGAGCTTTA 
CCTCCCGCAA GAGTCGGAGC AACAACACAG CCCTCTCCTG CGCCCCCCAC CAAGGATTCC 
TCAACAGTAT CCAGCACAGA CACGGACACA GACAAACTAA AATATGTTTC AAATTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750156.1
CDS	396..2915
Translation	MESKFNSSTNSSRDDGNNVLQGKAEKSLTASTSSVKEHGNSVCFKTDAADDPVVGFEDAEGPSRPHGFMQRQFGSLMQPGVNKFSLRMFGSQKAVEKEQERVKTAGVWIIHPYSDFRFYWDLIMLIMMVGNLVIIPVGITFFTEQTTTPWIIFNVASDTVFLFDLFMNFRTGIVIEDNSEIILDPKVIKMNYLKSWFVVDFISSIPVDYIFLIVEKGMDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIFNLIGMMLLLCHWDGCLQFLVPLLQEFPPDCWVSLNNMVNDSWGKQYSYALFKAMSHMLCIGYGARAPVSMSDLWITMLSMIVGATCYAMFVGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPSDMRQKIHDYYEHRYQGKIFDEDNILNELNDPLREEIVNFNCRKLVATMPLFANADPNFVTAMLSKLKFEVFQPGDYIIREGAVGKKMYFIQHGVAGVITKSSKEMKLTDGSYFGEICLLTKGRRTASVRAETYCRLYSLSVENFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILQQRFQKDLNTGIFNNQESEILKQMLTQDREMVEGHPSVSYQQLPALNSSTSSSLRMRTRSPPVYNANSLSHGNLQSPTPSPQTPQQAVVFSPSSFTSAACSPSVQSPLAGRTFQYSSLTASQLSLIQQQQQASGASQIHEVHKSAQALANTNLTREVRPLSASQPSLPHEISTLGSKPHPTVGESLASLPQPISNVQAAGTQSGSRSNIPPRVALFRQMSSGALPPARVGATTQPSPAPPTKDSSTVSSTDTDTDKLKYVSNL

Target ORF information:

RefSeq Version	XM_031894296.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis hyperpolarization activated cyclic nucleotide gated potassium channel 1 (hcn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894296.1

ORF Insert Sequence:

ATGGAAAGCA AATTCAACTC CTCCACCAAC AGCAGCAGGG ACGACGGCAA TAATGTGTTG 
CAGGGCAAAG CGGAGAAAAG TCTGACTGCC AGCACTTCCA GCGTCAAGGA GCATGGCAAC 
TCCGTCTGCT TCAAAACGGA CGCCGCCGAC GATCCCGTAG TTGGATTTGA GGACGCGGAA 
GGGCCCAGCC GGCCTCACGG CTTCATGCAG CGACAGTTCG GCTCCTTGAT GCAGCCGGGA 
GTCAATAAAT TCTCCCTCCG CATGTTTGGC AGTCAGAAGG CGGTGGAGAA GGAGCAGGAG 
AGGGTTAAAA CGGCAGGAGT GTGGATCATC CATCCCTACA GTGATTTCAG ATTTTACTGG 
GACCTGATCA TGCTCATCAT GATGGTCGGA AATCTTGTCA TAATACCAGT CGGAATCACG 
TTCTTCACAG AGCAAACAAC AACGCCGTGG ATTATCTTCA ATGTGGCCTC CGACACCGTC 
TTTTTATTTG ACTTGTTCAT GAATTTTAGA ACTGGAATTG TTATTGAGGA CAATTCAGAG 
ATAATCCTCG ATCCAAAAGT CATAAAAATG AATTATCTCA AAAGCTGGTT CGTAGTGGAC 
TTCATCTCCT CTATTCCAGT GGATTATATC TTTCTCATTG TCGAGAAAGG CATGGATTCG 
GAAGTGTACA AGACCGCTCG GGCGCTCCGC ATAGTGAGGT TTACAAAAAT TCTCAGTTTG 
CTGCGATTGC TGCGACTTTC GAGGTTAATT AGATATATTC ATCAGTGGGA AGAGATTTTC 
CATATGACTT ATGACTTGGC TAGTGCCGTG GTGAGAATCT TCAATCTCAT TGGCATGATG 
CTGCTGCTTT GTCACTGGGA TGGCTGCCTT CAGTTTTTGG TACCATTACT TCAGGAATTT 
CCACCAGACT GCTGGGTATC ATTAAATAAC ATGGTGAATG ATTCCTGGGG AAAACAGTAT 
TCCTATGCTC TCTTTAAAGC AATGAGCCAT ATGCTGTGCA TTGGATATGG GGCCCGTGCA 
CCTGTCAGCA TGTCCGATCT GTGGATCACT ATGTTGAGCA TGATTGTGGG AGCCACGTGC 
TATGCCATGT TTGTTGGACA TGCCACAGCC TTAATACAGT CCCTGGATTC TTCACGTCGC 
CAGTACCAAG AAAAGTACAA ACAAGTGGAA CAGTACATGT CATTTCACAA ATTGCCTTCT 
GATATGCGGC AGAAGATACA CGATTATTAT GAGCACCGAT ATCAAGGAAA AATATTCGAT 
GAAGACAACA TTCTTAATGA GCTAAATGAC CCATTGAGAG AGGAAATAGT CAACTTTAAC 
TGTCGGAAAC TGGTGGCTAC GATGCCTCTG TTTGCTAACG CGGATCCAAA CTTTGTCACA 
GCCATGCTGA GCAAACTGAA GTTTGAAGTG TTTCAGCCTG GAGATTACAT TATTCGTGAA 
GGAGCTGTGG GGAAGAAGAT GTATTTTATA CAGCATGGTG TGGCAGGTGT CATTACTAAA 
TCCAGCAAAG AAATGAAGTT AACTGATGGA TCTTATTTTG GAGAGATTTG CCTTTTGACA 
AAAGGTCGGA GAACTGCTAG CGTCCGAGCA GAAACCTATT GCCGCCTGTA TTCTCTTTCG 
GTGGAGAACT TCAATGAAGT GCTGGAAGAA TATCCTATGA TGAGACGAGC CTTTGAAACT 
GTGGCCATAG ACAGATTAGA CAGGATAGGG AAGAAGAACT CAATTCTTCA GCAACGATTT 
CAAAAGGACC TCAACACGGG AATATTCAAC AACCAGGAGA GCGAAATCCT GAAGCAGATG 
CTGACACAAG ACAGGGAGAT GGTGGAAGGT CATCCCTCAG TTAGCTACCA GCAGCTGCCA 
GCTCTAAATT CTAGCACAAG TTCATCATTG AGAATGAGGA CCCGATCTCC ACCTGTTTAT 
AATGCAAACA GTTTGTCTCA TGGAAACCTC CAATCACCAA CACCCAGCCC CCAGACGCCT 
CAACAAGCTG TTGTGTTTTC ACCTTCTTCC TTTACTTCTG CAGCCTGCAG TCCTTCAGTT 
CAGAGTCCAT TGGCTGGTCG AACTTTTCAG TATAGTTCAC TTACAGCATC ACAGCTTTCA 
TTAATTCAGC AACAGCAGCA AGCATCTGGC GCATCCCAGA TACATGAAGT TCATAAAAGC 
GCTCAAGCTC TTGCAAACAC TAACCTAACT CGAGAAGTGA GGCCTCTTTC AGCCTCACAG 
CCTTCTTTGC CACATGAGAT ATCTACTTTA GGATCTAAAC CACACCCGAC TGTGGGAGAA 
TCGTTAGCTT CACTCCCCCA GCCAATATCA AATGTTCAGG CTGCAGGTAC TCAGTCTGGA 
AGTCGGAGCA ATATCCCTCC AAGGGTAGCC CTTTTTCGGC AGATGTCTTC AGGAGCTTTA 
CCTCCCGCAA GAGTCGGAGC AACAACACAG CCCTCTCCTG CGCCCCCCAC CAAGGATTCC 
TCAACAGTAT CCAGCACAGA CACGGACACA GACAAACTAA AATATGTTTC AAATTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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hcn1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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