Actb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Actb
Entrez Gene ID	81822
Full Name	actin, beta
Synonyms	Actx
General protein information	Preferred Names actin, beta Names actin, cytoplasmic 1 beta-actin cytoplasmic beta-actin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12p11
Summary	mRNA expression increases following axon injury; may play a role in acceleration of axonal outgrowth [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031144.3	NP_112406.1	actin, cytoplasmic 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ACTB	NP_001092.1
Gallus gallus (chicken)	ACTB	NP_990849.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011516	XP_315269.4
Pan troglodytes (chimpanzee)	ACTB	NP_001009945.1
	LOC100428865	XP_002802770.1
Rattus norvegicus (Norway rat)	Actb	NP_112406.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005095	XP_313971.4
Oryza sativa (rice)	Os05g0438800	NP_001055661.1
Canis lupus familiaris (dog)	ACTB	NP_001182774.1
Drosophila melanogaster (fruit fly)	Act57B	NP_523800.1
Xenopus tropicalis (tropical clawed frog)	actb	NP_998884.1
	ACTB	NP_001028256.1
Mus musculus (house mouse)	Actb	NP_031419.1
Bos taurus (cattle)	ACTB	NP_776404.2
Drosophila melanogaster (fruit fly)	Act87E	NP_731812.1

Gene Ontology
Bibliography

Related articles in PubMed

Sperm Release at Spermiation Is Regulated by Changes in the Organization of Actin- and Microtubule-Based Cytoskeletons at the Apical Ectoplasmic Specialization-A Study Using the Adjudin Model.
Li L, Tang EI, Chen H, Lian Q, Ge R, Silvestrini B, Cheng CY
Endocrinology158(12)4300-4316(2017 Dec)

Type of supplemented simple sugar, not merely calorie intake, determines adverse effects on metabolism and aortic function in female rats.
Sang?esa G, Shaligram S, Akther F, Roglans N, Laguna JC, Rahimian R, Alegret M
American journal of physiology. Heart and circulatory physiology312(2)H289-H304(2017 Feb)

Actin Is Crucial for All Kinetically Distinguishable Forms of Endocytosis at Synapses.
Wu XS, Lee SH, Sheng J, Zhang Z, Zhao WD, Wang D, Jin Y, Charnay P, Ervasti JM, Wu LG
Neuron92(5)1020-1035(2016 Dec)

Coordination of Actin- and Microtubule-Based Cytoskeletons Supports Transport of Spermatids and Residual Bodies/Phagosomes During Spermatogenesis in the Rat Testis.
Tang EI, Lee WM, Cheng CY
Endocrinology157(4)1644-59(2016 Apr)

Upregulation of HO-1 with Haemin Alleviates LPS-Stimulated Pro-inflammatory Responses Through Downregulation of p38 Signalling Pathways in Rat Liver.
Tang J, Li L, Li CM, Wu J, Sun Y, Wang GL
Scandinavian journal of immunology82(5)443-51(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that spermiation is actin- and microtubule-based cytoskeletal-dependent event, involving regulatory proteins that modify cytoskeletal organization.
Title: Sperm Release at Spermiation Is Regulated by Changes in the Organization of Actin- and Microtubule-Based Cytoskeletons at the Apical Ectoplasmic Specialization-A Study Using the Adjudin Model.

Data show that spermatid/phagosome transport across the epithelium during spermatogenesis requires the coordination between actin- and microtubule (MT)-based cytoskeletons.
Title: Coordination of Actin- and Microtubule-Based Cytoskeletons Supports Transport of Spermatids and Residual Bodies/Phagosomes During Spermatogenesis in the Rat Testis.

Both ZBP1 and HuD bind the zipcode element in rat beta-actin mRNA's 3' UTR. Differences between HuD and ZBP1 were observed in their binding preference to the element.
Title: Different motif requirements for the localization zipcode element of ?-actin mRNA binding by HuD and ZBP1.

Thsi study validated the Hprt1, Actb, Mapk6 or B2m and showed that any one or combination of two of them are good reference genes for normalization of mRNA expression in qPCR experiments in the spinal cord and RVM in the CFA model of inflammatory injury.
Title: Validation of four reference genes for quantitative mRNA expression studies in a rat model of inflammatory injury.

Data indicate that LIM motif-containing protein kinases LIMK1 and LIMK2-dependent acin rearrangement is involved in the development of complete Freund's adjuvant (CFA)-induced inflammatory pain.
Title: LIMK-dependent actin polymerization in primary sensory neurons promotes the development of inflammatory heat hyperalgesia in rats.

The following Actb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Actb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07739	NM_031144.3 Latest version!	Rattus norvegicus actin, beta (Actb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Actb ( NM_031144.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112406.1 Rattus norvegicus actin, beta (Actb), mRNA.

Price & Availability↑

CloneID	ORa07739
Clone ID Related Accession (Same CDS sequence)	NM_031144.3
Accession Version	NM_031144.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	actin, cytoplasmic 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC063166.1. On Aug 31, 2012 this sequence version replaced NM_031144.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC063166.1, FQ231503.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGATGACG ATATCGCTGC GCTCGTCGTC GACAACGGCT CCGGCATGTG CAAGGCCGGC 
TTCGCGGGCG ACGATGCTCC CCGGGCCGTC TTCCCCTCCA TCGTGGGCCG CCCTAGGCAC 
CAGGGTGTGA TGGTGGGTAT GGGTCAGAAG GACTCCTACG TGGGCGACGA GGCCCAGAGC 
AAGAGAGGCA TCCTGACCCT GAAGTACCCC ATTGAACACG GCATTGTCAC CAACTGGGAC 
GATATGGAGA AGATTTGGCA CCACACTTTC TACAATGAGC TGCGTGTGGC CCCTGAGGAG 
CACCCTGTGC TGCTCACCGA GGCCCCTCTG AACCCTAAGG CCAACCGTGA AAAGATGACC 
CAGATCATGT TTGAGACCTT CAACACCCCA GCCATGTACG TAGCCATCCA GGCTGTGTTG 
TCCCTGTATG CCTCTGGTCG TACCACTGGC ATTGTGATGG ACTCCGGAGA CGGGGTCACC 
CACACTGTGC CCATCTATGA GGGTTACGCG CTCCCTCATG CCATCCTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACAGA CTACCTCATG AAGATCCTGA CCGAGCGTGG CTACAGCTTC 
ACCACCACAG CTGAGAGGGA AATCGTGCGT GACATTAAAG AGAAGCTGTG CTATGTTGCC 
CTAGACTTCG AGCAAGAGAT GGCCACTGCC GCATCCTCTT CCTCCCTGGA GAAGAGCTAT 
GAGCTGCCTG ACGGTCAGGT CATCACTATC GGCAATGAGC GGTTCCGATG CCCCGAGGCT 
CTCTTCCAGC CTTCCTTCCT GGGTATGGAA TCCTGTGGCA TCCATGAAAC TACATTCAAT 
TCCATCATGA AGTGTGACGT TGACATCCGT AAAGACCTCT ATGCCAACAC AGTGCTGTCT 
GGTGGCACCA CCATGTACCC AGGCATTGCT GACAGGATGC AGAAGGAGAT TACTGCCCTG 
GCTCCTAGCA CCATGAAGAT CAAGATCATT GCTCCTCCTG AGCGCAAGTA CTCTGTGTGG 
ATTGGTGGCT CTATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT CAGCAAGCAG 
GAGTACGATG AGTCCGGCCC CTCCATCGTG CACCGCAAAT GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112406.1
CDS	79..1206
Translation	MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_031144.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus actin, beta (Actb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031144.3

ORF Insert Sequence:

ATGGATGACG ATATCGCTGC GCTCGTCGTC GACAACGGCT CCGGCATGTG CAAGGCCGGC 
TTCGCGGGCG ACGATGCTCC CCGGGCCGTC TTCCCCTCCA TCGTGGGCCG CCCTAGGCAC 
CAGGGTGTGA TGGTGGGTAT GGGTCAGAAG GACTCCTACG TGGGCGACGA GGCCCAGAGC 
AAGAGAGGCA TCCTGACCCT GAAGTACCCC ATTGAACACG GCATTGTCAC CAACTGGGAC 
GATATGGAGA AGATTTGGCA CCACACTTTC TACAATGAGC TGCGTGTGGC CCCTGAGGAG 
CACCCTGTGC TGCTCACCGA GGCCCCTCTG AACCCTAAGG CCAACCGTGA AAAGATGACC 
CAGATCATGT TTGAGACCTT CAACACCCCA GCCATGTACG TAGCCATCCA GGCTGTGTTG 
TCCCTGTATG CCTCTGGTCG TACCACTGGC ATTGTGATGG ACTCCGGAGA CGGGGTCACC 
CACACTGTGC CCATCTATGA GGGTTACGCG CTCCCTCATG CCATCCTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACAGA CTACCTCATG AAGATCCTGA CCGAGCGTGG CTACAGCTTC 
ACCACCACAG CTGAGAGGGA AATCGTGCGT GACATTAAAG AGAAGCTGTG CTATGTTGCC 
CTAGACTTCG AGCAAGAGAT GGCCACTGCC GCATCCTCTT CCTCCCTGGA GAAGAGCTAT 
GAGCTGCCTG ACGGTCAGGT CATCACTATC GGCAATGAGC GGTTCCGATG CCCCGAGGCT 
CTCTTCCAGC CTTCCTTCCT GGGTATGGAA TCCTGTGGCA TCCATGAAAC TACATTCAAT 
TCCATCATGA AGTGTGACGT TGACATCCGT AAAGACCTCT ATGCCAACAC AGTGCTGTCT 
GGTGGCACCA CCATGTACCC AGGCATTGCT GACAGGATGC AGAAGGAGAT TACTGCCCTG 
GCTCCTAGCA CCATGAAGAT CAAGATCATT GCTCCTCCTG AGCGCAAGTA CTCTGTGTGG 
ATTGGTGGCT CTATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT CAGCAAGCAG 
GAGTACGATG AGTCCGGCCC CTCCATCGTG CACCGCAAAT GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sperm Release at Spermiation Is Regulated by Changes in the Organization of Actin- and Microtubule-Based Cytoskeletons at the Apical Ectoplasmic Specialization-A Study Using the Adjudin Model.
Endocrinology158(12)4300-4316(2017 Dec)
Li L,Tang EI,Chen H,Lian Q,Ge R,Silvestrini B,Cheng CY

Type of supplemented simple sugar, not merely calorie intake, determines adverse effects on metabolism and aortic function in female rats.
American journal of physiology. Heart and circulatory physiology312(2)H289-H304(2017 Feb)
Sang?esa G,Shaligram S,Akther F,Roglans N,Laguna JC,Rahimian R,Alegret M

Actin Is Crucial for All Kinetically Distinguishable Forms of Endocytosis at Synapses.
Neuron92(5)1020-1035(2016 Dec)
Wu XS,Lee SH,Sheng J,Zhang Z,Zhao WD,Wang D,Jin Y,Charnay P,Ervasti JM,Wu LG

Coordination of Actin- and Microtubule-Based Cytoskeletons Supports Transport of Spermatids and Residual Bodies/Phagosomes During Spermatogenesis in the Rat Testis.
Endocrinology157(4)1644-59(2016 Apr)
Tang EI,Lee WM,Cheng CY

Upregulation of HO-1 with Haemin Alleviates LPS-Stimulated Pro-inflammatory Responses Through Downregulation of p38 Signalling Pathways in Rat Liver.
Scandinavian journal of immunology82(5)443-51(2015 Nov)
Tang J,Li L,Li CM,Wu J,Sun Y,Wang GL

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Actb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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