Pde1a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pde1a
Entrez Gene ID	81529
Full Name	phosphodiesterase 1A
Synonyms	3'-RACEclone8phosphodiesterase1A,3-RACEclone8phosphodiesterase1A
General protein information	Preferred Names phosphodiesterase 1A Names calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A 3'-RACE clone 8 phosphodiesterase 1A 3-RACE clone 8 phosphodiesterase 1A phosphodiesterase 1A, calmodulin-dependent
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q24
Summary	expression in the hippocampus increases in response to electroconvulsive shock [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_030871.1	NP_110498.1	calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PDE1A	NP_776839.1
Danio rerio (zebrafish)	pde1a	XP_005167982.1
Xenopus tropicalis (tropical clawed frog)	pde1a	NP_001096444.1
Pan troglodytes (chimpanzee)	PDE1A	XP_515959.3
	PDE1A	XP_001101568.1
Rattus norvegicus (Norway rat)	Pde1a	NP_110498.1
Homo sapiens (human)	PDE1A	NP_001245241.1
Drosophila melanogaster (fruit fly)	Pde1c	NP_001245994.1
Canis lupus familiaris (dog)	PDE1A	NP_001003091.1
Mus musculus (house mouse)	Pde1a	NP_058024.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008967	XP_319716.3
Gallus gallus (chicken)	PDE1A	XP_004942743.1

Gene Ontology
Bibliography

Related articles in PubMed

PDE1A inhibition elicits cGMP-dependent relaxation of rat mesenteric arteries.
Khammy MM, Dalsgaard T, Larsen PH, Christoffersen CT, Clausen D, Rasmussen LK, Folkersen L, Grunnet M, Kehler J, Aalkjaer C, Nielsen J
British journal of pharmacology174(22)4186-4198(2017 Nov)

Cyclic nucleotide phosphodiesterase 1A: a key regulator of cardiac fibroblast activation and extracellular matrix remodeling in the heart.
Miller CL, Cai Y, Oikawa M, Thomas T, Dostmann WR, Zaccolo M, Fujiwara K, Yan C
Basic research in cardiology106(6)1023-39(2011 Nov)

Decreased cGMP level contributes to increased contraction in arteries from hypertensive rats: role of phosphodiesterase 1.
Giachini FR, Lima VV, Carneiro FS, Tostes RC, Webb RC
Hypertension (Dallas, Tex. : 1979)57(3)655-63(2011 Mar)

Ca2+/calmodulin-stimulated PDE1 regulates the beta-catenin/TCF signaling through PP2A B56 gamma subunit in proliferating vascular smooth muscle cells.
Jeon KI, Jono H, Miller CL, Cai Y, Lim S, Liu X, Gao P, Abe J, Li JD, Yan C
The FEBS journal277(24)5026-39(2010 Dec)

Intrinsic sex-specific differences in microvascular endothelial cell phosphodiesterases.
Wang J, Bingaman S, Huxley VH
American journal of physiology. Heart and circulatory physiology298(4)H1146-54(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that induction of PDE1A plays a critical role in cardiac fibroblast activation and cardiac fibrosis
Title: Cyclic nucleotide phosphodiesterase 1A: a key regulator of cardiac fibroblast activation and extracellular matrix remodeling in the heart.

We conclude that PDE1 activation reduces cGMP bioavailability in arteries from Ang II, contributing to increased contractile responsiveness.
Title: Decreased cGMP level contributes to increased contraction in arteries from hypertensive rats: role of phosphodiesterase 1.

findings provide direct evidence for the first time that PP2A B56gamma is a critical mediator for PDE1A in the regulation of beta-catenin signaling in proliferating VSMCs
Title: Ca2+/calmodulin-stimulated PDE1 regulates the beta-catenin/TCF signaling through PP2A B56 gamma subunit in proliferating vascular smooth muscle cells.

Expression mRNA levels of PDE1A (male > female) and PDE3B (male < female) were sex dependent in microvascular endothelial cells.
Title: Intrinsic sex-specific differences in microvascular endothelial cell phosphodiesterases.

a novel role for Ca(2+)/CaM-stimulated PDE1, particularly PDE1A, in regulating pathological cardiomyocyte hypertrophy via a cGMP/PKG-dependent mechanism, thereby demonstrating Ca(2+) and cGMP signaling cross-talk during cardiac hypertrophy.
Title: Role of Ca2+/calmodulin-stimulated cyclic nucleotide phosphodiesterase 1 in mediating cardiomyocyte hypertrophy.

The following Pde1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pde1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07258	NM_030871.1 Latest version!	Rattus norvegicus phosphodiesterase 1A (Pde1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pde1a ( NM_030871.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_110498.1 Rattus norvegicus phosphodiesterase 1A (Pde1a), mRNA.

Price & Availability↑

CloneID	ORa07258
Clone ID Related Accession (Same CDS sequence)	NM_030871.1
Accession Version	NM_030871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Rattus norvegicus(Norway rat)
Product	calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF327838.1. On Apr 19, 2005 this sequence version replaced XM_579497.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF327838.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGGCTCTA CAGATACAGA CATCGAAGAA CTGGAAAACG CCACTTACAA GTATCTCATT 
GGAGAACAGA CCGAGAAGAT GTGGCAACGC CTGAAAGGAA TACTAAGATG CTTAGTGAAG 
CAGCTGGAGA AAGGTGATGT CAACGTTGTC GACCTAAAGA AGAATATCGA ATATGCGGCA 
TCTGTACTGG AAGCTGTTTA CATCGACGAG ACGAGGAGGC TTCTGGATAC TGAGGATGAG 
CTCAGCGACA TTCAGACCGA CTCGGTTCCT TCGGAAGTCC GGGACTGGTT GGCTTCAACC 
TTTACACGGA AAATGGGGAT GACGAAAAAG AAACCCGAGG AAAAACCGAA GTTTCGCAGC 
ATTGTCCACG CTGTCCAGGC TGGGATTTTT GTGGAAAGGA TGTACAGGAA GAACTATCAC 
ATGGTTGGTT TGACATATCC TGCAGCTGTC ATAGTGACAC TCAAGGAAGT TGATAAATGG 
TCTTTTGATG TATTTGCCTT AAATGAAGCA AGCGGGGAGC ATAGTCTGAA GTTTATGATT 
TATGAACTAT TTACCAGTAA TGATCTTATC AACCGCTTCA AGATTCCTGT TTCTTGCCTA 
ATTGCCTTTG CAGAAGCTTT AGAAGTTGGT TACAGCAAGC ACAAAAATCC GTATCATAAT 
TTGGTCCATG CAGCTGATGT CACTCAAACT GTGCATTACA TAATGCTTCA TACAGGTATC 
ATGCACTGGC TCACTGAACT GGAAATCTTA GCCATGGTCT TTGCAGCCGC CGTTCATGAC 
TATGAACACA CAGGAACTAC AAACAACTTC CATATTCAGA CGAGGTCAGA CGTCGCCATT 
CTGTATAACG ACCGCTCAGT TCTTGAGAAT CACCATGTCA GCGCAGCCTA CAGACTTATG 
CAAGAGGAGG AAATGAATAT TTTGGTAAAT TTATCTAAAG ATGACTGGAG GGATCTTCGG 
AACTTAGTGA TTGAAACGGT CTTAGCAACA GACATGTCAG GCCATTTCCA GCAAATTAAA 
AACATAAGAA GCAGCTTGCA GCAGCCTGAA GGGATTGACA GAGCCAAAAC GATGTCCCTG 
ATACTCCACG CAGCCGACAT CAGCCACCCA GCCAAAACGT GGAAGCTGCA CTACAGGTGG 
ACTATGGCCT TAATGGAGGA ATTTTTCCTG CAGGGAGACA AAGAAGCTGA ATTAGGGCTT 
CCGTTTTCTC CACTTTGTGA TCGGAAGTCA ACGATGGTTG CCCAGTCTCA AATAGGTTTC 
ATTGATTTCA TAGTGGAGCC AACGTTTTCT CTCCTGACAG ACTCGACAGA GAAAATTGTT 
ATTCCTCTTA TAGAGGAAGC TTCAAAACCA GAGTCTTCTA ACTATGGGGC AAGCAGCTCG 
TCCACCATGA TTGGGTTCCA TGTTGCTGAC GCTCTGAGGC GGTCAAACAC AAAAGGCTGC 
ATGAGTGATG GGACCTATGC CCCAGACTAC TCTCTGTCAG CTGTGGACCT GAAGAGCTTC 
AAAAACAACC TGGTGGACAT CATTCAGCAG AACAAGGAGA GGTGGAAAGA GTTGGCTGCC 
CAAGGCGAAC TTGATCTTCA TAAGAACTCA GAAGACTTAG AAGAAAAGCA TGCTGACACA 
CATCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_110498.1
CDS	77..1705
Translation	MGSTDTDIEELENATYKYLIGEQTEKMWQRLKGILRCLVKQLEKGDVNVVDLKKNIEYAASVLEAVYIDETRRLLDTEDELSDIQTDSVPSEVRDWLASTFTRKMGMTKKKPEEKPKFRSIVHAVQAGIFVERMYRKNYHMVGLTYPAAVIVTLKEVDKWSFDVFALNEASGEHSLKFMIYELFTSNDLINRFKIPVSCLIAFAEALEVGYSKHKNPYHNLVHAADVTQTVHYIMLHTGIMHWLTELEILAMVFAAAVHDYEHTGTTNNFHIQTRSDVAILYNDRSVLENHHVSAAYRLMQEEEMNILVNLSKDDWRDLRNLVIETVLATDMSGHFQQIKNIRSSLQQPEGIDRAKTMSLILHAADISHPAKTWKLHYRWTMALMEEFFLQGDKEAELGLPFSPLCDRKSTMVAQSQIGFIDFIVEPTFSLLTDSTEKIVIPLIEEASKPESSNYGASSSSTMIGFHVADALRRSNTKGCMSDGTYAPDYSLSAVDLKSFKNNLVDIIQQNKERWKELAAQGELDLHKNSEDLEEKHADTHP

Target ORF information:

RefSeq Version	NM_030871.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 1A (Pde1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030871.1

ORF Insert Sequence:

ATGGGCTCTA CAGATACAGA CATCGAAGAA CTGGAAAACG CCACTTACAA GTATCTCATT 
GGAGAACAGA CCGAGAAGAT GTGGCAACGC CTGAAAGGAA TACTAAGATG CTTAGTGAAG 
CAGCTGGAGA AAGGTGATGT CAACGTTGTC GACCTAAAGA AGAATATCGA ATATGCGGCA 
TCTGTACTGG AAGCTGTTTA CATCGACGAG ACGAGGAGGC TTCTGGATAC TGAGGATGAG 
CTCAGCGACA TTCAGACCGA CTCGGTTCCT TCGGAAGTCC GGGACTGGTT GGCTTCAACC 
TTTACACGGA AAATGGGGAT GACGAAAAAG AAACCCGAGG AAAAACCGAA GTTTCGCAGC 
ATTGTCCACG CTGTCCAGGC TGGGATTTTT GTGGAAAGGA TGTACAGGAA GAACTATCAC 
ATGGTTGGTT TGACATATCC TGCAGCTGTC ATAGTGACAC TCAAGGAAGT TGATAAATGG 
TCTTTTGATG TATTTGCCTT AAATGAAGCA AGCGGGGAGC ATAGTCTGAA GTTTATGATT 
TATGAACTAT TTACCAGTAA TGATCTTATC AACCGCTTCA AGATTCCTGT TTCTTGCCTA 
ATTGCCTTTG CAGAAGCTTT AGAAGTTGGT TACAGCAAGC ACAAAAATCC GTATCATAAT 
TTGGTCCATG CAGCTGATGT CACTCAAACT GTGCATTACA TAATGCTTCA TACAGGTATC 
ATGCACTGGC TCACTGAACT GGAAATCTTA GCCATGGTCT TTGCAGCCGC CGTTCATGAC 
TATGAACACA CAGGAACTAC AAACAACTTC CATATTCAGA CGAGGTCAGA CGTCGCCATT 
CTGTATAACG ACCGCTCAGT TCTTGAGAAT CACCATGTCA GCGCAGCCTA CAGACTTATG 
CAAGAGGAGG AAATGAATAT TTTGGTAAAT TTATCTAAAG ATGACTGGAG GGATCTTCGG 
AACTTAGTGA TTGAAACGGT CTTAGCAACA GACATGTCAG GCCATTTCCA GCAAATTAAA 
AACATAAGAA GCAGCTTGCA GCAGCCTGAA GGGATTGACA GAGCCAAAAC GATGTCCCTG 
ATACTCCACG CAGCCGACAT CAGCCACCCA GCCAAAACGT GGAAGCTGCA CTACAGGTGG 
ACTATGGCCT TAATGGAGGA ATTTTTCCTG CAGGGAGACA AAGAAGCTGA ATTAGGGCTT 
CCGTTTTCTC CACTTTGTGA TCGGAAGTCA ACGATGGTTG CCCAGTCTCA AATAGGTTTC 
ATTGATTTCA TAGTGGAGCC AACGTTTTCT CTCCTGACAG ACTCGACAGA GAAAATTGTT 
ATTCCTCTTA TAGAGGAAGC TTCAAAACCA GAGTCTTCTA ACTATGGGGC AAGCAGCTCG 
TCCACCATGA TTGGGTTCCA TGTTGCTGAC GCTCTGAGGC GGTCAAACAC AAAAGGCTGC 
ATGAGTGATG GGACCTATGC CCCAGACTAC TCTCTGTCAG CTGTGGACCT GAAGAGCTTC 
AAAAACAACC TGGTGGACAT CATTCAGCAG AACAAGGAGA GGTGGAAAGA GTTGGCTGCC 
CAAGGCGAAC TTGATCTTCA TAAGAACTCA GAAGACTTAG AAGAAAAGCA TGCTGACACA 
CATCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PDE1A inhibition elicits cGMP-dependent relaxation of rat mesenteric arteries.
British journal of pharmacology174(22)4186-4198(2017 Nov)
Khammy MM,Dalsgaard T,Larsen PH,Christoffersen CT,Clausen D,Rasmussen LK,Folkersen L,Grunnet M,Kehler J,Aalkjaer C,Nielsen J

Cyclic nucleotide phosphodiesterase 1A: a key regulator of cardiac fibroblast activation and extracellular matrix remodeling in the heart.
Basic research in cardiology106(6)1023-39(2011 Nov)
Miller CL,Cai Y,Oikawa M,Thomas T,Dostmann WR,Zaccolo M,Fujiwara K,Yan C

Decreased cGMP level contributes to increased contraction in arteries from hypertensive rats: role of phosphodiesterase 1.
Hypertension (Dallas, Tex. : 1979)57(3)655-63(2011 Mar)
Giachini FR,Lima VV,Carneiro FS,Tostes RC,Webb RC

Ca2+/calmodulin-stimulated PDE1 regulates the beta-catenin/TCF signaling through PP2A B56 gamma subunit in proliferating vascular smooth muscle cells.
The FEBS journal277(24)5026-39(2010 Dec)
Jeon KI,Jono H,Miller CL,Cai Y,Lim S,Liu X,Gao P,Abe J,Li JD,Yan C

Intrinsic sex-specific differences in microvascular endothelial cell phosphodiesterases.
American journal of physiology. Heart and circulatory physiology298(4)H1146-54(2010 Apr)
Wang J,Bingaman S,Huxley VH