Pou2af1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pou2af1
Entrez Gene ID	690528
Full Name	POU class 2 associating factor 1
General protein information	Preferred Names POU class 2 associating factor 1 Names POU domain class 2-associating factor 1 POU domain, class 2, associating factor 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q23

mRNA and Protein(s)

mRNA	Protein	Name
XM_008766341.2	XP_008764563.1	POU domain class 2-associating factor 1 isoform X1
NM_001109599.1	NP_001103069.1	POU domain class 2-associating factor 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	POU2AF1	XP_546531.2
Gallus gallus (chicken)	POU2AF1	NP_989506.1
Pan troglodytes (chimpanzee)	POU2AF1	XP_508747.2
Mus musculus (house mouse)	Pou2af1	NP_035266.1
Bos taurus (cattle)	POU2AF1	NP_001069383.1
Rattus norvegicus (Norway rat)	Pou2af1	NP_001103069.1
Xenopus tropicalis (tropical clawed frog)	pou2af1	XP_002932979.2
Homo sapiens (human)	POU2AF1	NP_006226.2

Gene Ontology
Bibliography

Related articles in PubMed

Oct-2 regulates CD36 gene expression via a consensus octamer, which excludes the co-activator OBF-1.
Shore P, Dietrich W, Corcoran LM
Nucleic acids research30(8)1767-73(2002 Apr)

Expression of the Oct-1 transcription factor and characterization of its interactions with the Bob1 coactivator.
Lee L, Stollar E, Chang J, Grossmann JG, O'Brien R, Ladbury J, Carpenter B, Roberts S, Luisi B
Biochemistry40(22)6580-8(2001 Jun)

Crystal structure of an OCA-B peptide bound to an Oct-1 POU domain/octamer DNA complex: specific recognition of a protein-DNA interface.
Chasman D, Cepek K, Sharp PA, Pabo CO
Genes & development13(20)2650-7(1999 Oct)

Oct-1 POU and octamer DNA co-operate to recognise the Bob-1 transcription co-activator via induced folding.
Chang JF, Phillips K, Lundb?ck T, Gstaiger M, Ladbury JE, Luisi B
Journal of molecular biology288(5)941-52(1999 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pou2af1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pou2af1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10480	NM_001109599.1 Latest version!	Rattus norvegicus POU class 2 homeobox associating factor 1 (Pou2af1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa50499	XM_008766341.2 Latest version!	Rattus norvegicus POU class 2 associating factor 1 (Pou2af1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pou2af1 ( NM_001109599.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001103069.1 Rattus norvegicus POU class 2 homeobox associating factor 1 (Pou2af1), mRNA.

Price & Availability↑

CloneID	ORa10480
Clone ID Related Accession (Same CDS sequence)	NM_001109599.1
Accession Version	NM_001109599.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Rattus norvegicus(Norway rat)
Product	POU domain class 2-associating factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473975.1. On Oct 4, 2007 this sequence version replaced XM_001074701.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689594, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGCTCTGGC AAAAATCCAC AGCTTCAGAG CAAGCTCCTG CCCCTCCACG GCCATACCAG 
GGCGTTCGAG TCAAGGAGCC GGTGAAGGAG CTACTGAGAA GAAAGCGTGG CCATACCAGT 
GTTGGGACAG CTGGTCCACC AGCTGCGGTG GTACTGCCCC ACCAGCCCCT GGCAACCTAC 
AGCACAGTGG GTCCTTCCTG CCTTGACATG GAGGTTTCTG CTCCCACAGT GACAGAGGAG 
GGGACATTAT GTGCTGGCTG GCTCTCCCAA CCTGCCCCGG CCACTCTTCA ACCCTTGGCT 
CCATGGACGC CCTACACGGA GTATGTGTCC CATGAAGCTG TCAGCTGCCC TTACTCCGCT 
GACATGTATG TGCAGCCTGT GTGCCCCAGC TATACAGTGG TGGGACCCTC CTCGGTATTG 
ACCTATGCCT CCCCACCACT CATCACTAAC GTCACGCCAA GAAGCACGGC TACACCCGCA 
GTGGGGCCGC AGCTGGAGGG CCCAGAGCAC CAGGCGCCCC TTACCTATTT CCCGTGGCCT 
CAGCCCCTTT CCACACTGCC CACCTCCAGT CTGCAGTATC AACCTCCAGC CCCAACCCTG 
TCTGGGCCCC AGTTTGTCCA GCTCCCCATT TCTATCCCAG AGCCAGTCCT TCAGGACATG 
GATGACCCCA GAAGGGCCAT CAGCTCTCTG ACCATTGACA AGCTTCTTCT GGAGGAAGAG 
GAGAGCAACG CTTACGAGCT CAACCACACC CTCTCCGTGG AGGGCTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103069.1
CDS	97..867
Translation	MLWQKSTASEQAPAPPRPYQGVRVKEPVKELLRRKRGHTSVGTAGPPAAVVLPHQPLATYSTVGPSCLDMEVSAPTVTEEGTLCAGWLSQPAPATLQPLAPWTPYTEYVSHEAVSCPYSADMYVQPVCPSYTVVGPSSVLTYASPPLITNVTPRSTATPAVGPQLEGPEHQAPLTYFPWPQPLSTLPTSSLQYQPPAPTLSGPQFVQLPISIPEPVLQDMDDPRRAISSLTIDKLLLEEEESNAYELNHTLSVEGF

Target ORF information:

RefSeq Version	NM_001109599.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus POU class 2 homeobox associating factor 1 (Pou2af1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109599.1

ORF Insert Sequence:

ATGCTCTGGC AAAAATCCAC AGCTTCAGAG CAAGCTCCTG CCCCTCCACG GCCATACCAG 
GGCGTTCGAG TCAAGGAGCC GGTGAAGGAG CTACTGAGAA GAAAGCGTGG CCATACCAGT 
GTTGGGACAG CTGGTCCACC AGCTGCGGTG GTACTGCCCC ACCAGCCCCT GGCAACCTAC 
AGCACAGTGG GTCCTTCCTG CCTTGACATG GAGGTTTCTG CTCCCACAGT GACAGAGGAG 
GGGACATTAT GTGCTGGCTG GCTCTCCCAA CCTGCCCCGG CCACTCTTCA ACCCTTGGCT 
CCATGGACGC CCTACACGGA GTATGTGTCC CATGAAGCTG TCAGCTGCCC TTACTCCGCT 
GACATGTATG TGCAGCCTGT GTGCCCCAGC TATACAGTGG TGGGACCCTC CTCGGTATTG 
ACCTATGCCT CCCCACCACT CATCACTAAC GTCACGCCAA GAAGCACGGC TACACCCGCA 
GTGGGGCCGC AGCTGGAGGG CCCAGAGCAC CAGGCGCCCC TTACCTATTT CCCGTGGCCT 
CAGCCCCTTT CCACACTGCC CACCTCCAGT CTGCAGTATC AACCTCCAGC CCCAACCCTG 
TCTGGGCCCC AGTTTGTCCA GCTCCCCATT TCTATCCCAG AGCCAGTCCT TCAGGACATG 
GATGACCCCA GAAGGGCCAT CAGCTCTCTG ACCATTGACA AGCTTCTTCT GGAGGAAGAG 
GAGAGCAACG CTTACGAGCT CAACCACACC CTCTCCGTGG AGGGCTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pou2af1 ( XM_008766341.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764563.1 Rattus norvegicus POU class 2 associating factor 1 (Pou2af1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa50499
Clone ID Related Accession (Same CDS sequence)	XM_008766341.2
Accession Version	XM_008766341.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	POU domain class 2-associating factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766341.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAATA GCAGTGAAGG CCGAGAGGTG AAACAGGCAA GGGCATGCAG AGCCACAGCT 
TCAGAGCAAG CTCCTGCCCC TCCACGGCCA TACCAGGGCG TTCGAGTCAA GGAGCCGGTG 
AAGGAGCTAC TGAGAAGAAA GCGTGGCCAT ACCAGTGTTG GGACAGCTGG TCCACCAGCT 
GCGGTGGTAC TGCCCCACCA GCCCCTGGCA ACCTACAGCA CAGTGGGTCC TTCCTGCCTT 
GACATGGAGG TTTCTGCTCC CACAGTGACA GAGGAGGGGA CATTATGTGC TGGCTGGCTC 
TCCCAACCTG CCCCGGCCAC TCTTCAACCC TTGGCTCCAT GGACGCCCTA CACGGAGTAT 
GTGTCCCATG AAGCTGTCAG CTGCCCTTAC TCCGCTGACA TGTATGTGCA GCCTGTGTGC 
CCCAGCTATA CAGTGGTGGG ACCCTCCTCG GTATTGACCT ATGCCTCCCC ACCACTCATC 
ACTAACGTCA CGCCAAGAAG CACGGCTACA CCCGCAGTGG GGCCGCAGCT GGAGGGCCCA 
GAGCACCAGG CGCCCCTTAC CTATTTCCCG TGGCCTCAGC CCCTTTCCAC ACTGCCCACC 
TCCAGTCTGC AGTATCAACC TCCAGCCCCA ACCCTGTCTG GGCCCCAGTT TGTCCAGCTC 
CCCATTTCTA TCCCAGAGCC AGTCCTTCAG GACATGGATG ACCCCAGAAG GGCCATCAGC 
TCTCTGACCA TTGACAAGCT TCTTCTGGAG GAAGAGGAGA GCAACGCTTA CGAGCTCAAC 
CACACCCTCT CCGTGGAGGG CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764563.1
CDS	4104..4910
Translation	MRNSSEGREVKQARACRATASEQAPAPPRPYQGVRVKEPVKELLRRKRGHTSVGTAGPPAAVVLPHQPLATYSTVGPSCLDMEVSAPTVTEEGTLCAGWLSQPAPATLQPLAPWTPYTEYVSHEAVSCPYSADMYVQPVCPSYTVVGPSSVLTYASPPLITNVTPRSTATPAVGPQLEGPEHQAPLTYFPWPQPLSTLPTSSLQYQPPAPTLSGPQFVQLPISIPEPVLQDMDDPRRAISSLTIDKLLLEEEESNAYELNHTLSVEGF

Target ORF information:

RefSeq Version	XM_008766341.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus POU class 2 associating factor 1 (Pou2af1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766341.2

ORF Insert Sequence:

ATGAGGAATA GCAGTGAAGG CCGAGAGGTG AAACAGGCAA GGGCATGCAG AGCCACAGCT 
TCAGAGCAAG CTCCTGCCCC TCCACGGCCA TACCAGGGCG TTCGAGTCAA GGAGCCGGTG 
AAGGAGCTAC TGAGAAGAAA GCGTGGCCAT ACCAGTGTTG GGACAGCTGG TCCACCAGCT 
GCGGTGGTAC TGCCCCACCA GCCCCTGGCA ACCTACAGCA CAGTGGGTCC TTCCTGCCTT 
GACATGGAGG TTTCTGCTCC CACAGTGACA GAGGAGGGGA CATTATGTGC TGGCTGGCTC 
TCCCAACCTG CCCCGGCCAC TCTTCAACCC TTGGCTCCAT GGACGCCCTA CACGGAGTAT 
GTGTCCCATG AAGCTGTCAG CTGCCCTTAC TCCGCTGACA TGTATGTGCA GCCTGTGTGC 
CCCAGCTATA CAGTGGTGGG ACCCTCCTCG GTATTGACCT ATGCCTCCCC ACCACTCATC 
ACTAACGTCA CGCCAAGAAG CACGGCTACA CCCGCAGTGG GGCCGCAGCT GGAGGGCCCA 
GAGCACCAGG CGCCCCTTAC CTATTTCCCG TGGCCTCAGC CCCTTTCCAC ACTGCCCACC 
TCCAGTCTGC AGTATCAACC TCCAGCCCCA ACCCTGTCTG GGCCCCAGTT TGTCCAGCTC 
CCCATTTCTA TCCCAGAGCC AGTCCTTCAG GACATGGATG ACCCCAGAAG GGCCATCAGC 
TCTCTGACCA TTGACAAGCT TCTTCTGGAG GAAGAGGAGA GCAACGCTTA CGAGCTCAAC 
CACACCCTCT CCGTGGAGGG CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Oct-2 regulates CD36 gene expression via a consensus octamer, which excludes the co-activator OBF-1.
Nucleic acids research30(8)1767-73(2002 Apr)
Shore P,Dietrich W,Corcoran LM

Expression of the Oct-1 transcription factor and characterization of its interactions with the Bob1 coactivator.
Biochemistry40(22)6580-8(2001 Jun)
Lee L,Stollar E,Chang J,Grossmann JG,O'Brien R,Ladbury J,Carpenter B,Roberts S,Luisi B

Crystal structure of an OCA-B peptide bound to an Oct-1 POU domain/octamer DNA complex: specific recognition of a protein-DNA interface.
Genes & development13(20)2650-7(1999 Oct)
Chasman D,Cepek K,Sharp PA,Pabo CO

Oct-1 POU and octamer DNA co-operate to recognise the Bob-1 transcription co-activator via induced folding.
Journal of molecular biology288(5)941-52(1999 May)
Chang JF,Phillips K,Lundb?ck T,Gstaiger M,Ladbury JE,Luisi B