Oaz3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Oaz3
Entrez Gene ID	689588
Full Name	ornithine decarboxylase antizyme 3
Synonyms	Az3,ODC-Az 3,Oaz-t
General protein information	Preferred Names ornithine decarboxylase antizyme 3 Names ornithine decarboxylase antizyme 3 antizyme 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34
Summary	The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamines. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis; thus, completing the auto-regulatory circuit. This gene encodes antizyme 3, the third member of the antizyme family. Like antizymes 1 and 2, antizyme 3 inhibits ODC activity and polyamine uptake; however, it does not stimulate ODC degradation. Also, while antizymes 1 and 2 have broad tissue distribution, expression of antizyme 3 is restricted to haploid germ cells in testis, suggesting a distinct role for this antizyme in spermiogenesis. Antizyme 3 gene knockout studies showed that homozygous mutant male mice were infertile, and indicated the likely role of this antizyme in the formation of a rigid connection between the sperm head and tail during spermatogenesis. This transcript initiates translation from a non-AUG (CUG) codon that is highly conserved among the antizyme 3 orthologs. [provided by RefSeq, Dec 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001101018.1	NP_001094488.1	ornithine decarboxylase antizyme 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	OAZ3	NP_001166042.1
	OAZ3	NP_001122102.1
Canis lupus familiaris (dog)	OAZ3	NP_001128423.1
Mus musculus (house mouse)	Oaz3	NP_058597.2
Homo sapiens (human)	OAZ3	NP_057262.2
Bos taurus (cattle)	OAZ3	NP_001166179.1
Rattus norvegicus (Norway rat)	Oaz3	NP_001094488.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural and degradative aspects of ornithine decarboxylase antizyme inhibitor 2.
Ramos-Molina B, Lambertos A, Lopez-Contreras AJ, Kasprzak JM, Czerwoniec A, Bujnicki JM, Cremades A, Pe?afiel R
FEBS open bio4510-21(2014)

Proteomic analysis of microvesicles from plasma of healthy donors reveals high individual variability.
Bastos-Amador P, Royo F, Gonzalez E, Conde-Vancells J, Palomo-Diez L, Borras FE, Falcon-Perez JM
Journal of proteomics75(12)3574-84(2012 Jun)

Evidence of a role for antizyme and antizyme inhibitor as regulators of human cancer.
Olsen RR, Zetter BR
Molecular cancer research : MCR9(10)1285-93(2011 Oct)

Ornithine decarboxylase antizyme Oaz3 modulates protein phosphatase activity.
Ruan Y, Cheng M, Ou Y, Oko R, van der Hoorn FA
The Journal of biological chemistry286(33)29417-27(2011 Aug)

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ornithine decarboxylase antizyme Oaz3 modulates protein phosphatase activity.
Title: Ornithine decarboxylase antizyme Oaz3 modulates protein phosphatase activity.

N-terminal protein sequencing suggests translation initiation from a non-AUG (CUG) start site.
Title: Mammalian transcription in support of hybrid mRNA and protein synthesis in testis and lung.

The following Oaz3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Oaz3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa63261	NM_001101018.1 Latest version!	Rattus norvegicus ornithine decarboxylase antizyme 3 (Oaz3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Oaz3 ( NM_001101018.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001094488.1 Rattus norvegicus ornithine decarboxylase antizyme 3 (Oaz3), mRNA.

Price & Availability↑

CloneID	ORa63261
Clone ID Related Accession (Same CDS sequence)	NM_001101018.1
Accession Version	NM_001101018.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Rattus norvegicus(Norway rat)
Product	ornithine decarboxylase antizyme 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ431008.1 and AW529628.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ431008.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02642708, SAMN02642712 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 17040916 ribosomal slippage :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

CTGCCTTGTA CCAGGTCCCG CCCCTCTCTC TACTCCCTTT CTTATATTAA GAGGGGAAAA 
ACACGGAACT GCCTCTACCC ATTCTGGTCA CCATACGCCT ATTACCTCTA CTGTTACAAA 
TACCGGATCA CCCTCCGGGA GAAGATGCTG CCTTGTTGTT ACAGAAGCAT CACTTACAAG 
GAACAGGAGG ACCTGACTCT CCGGCCCCAT TGCTGCCTCC CGTGCTCCTG CCTCCCGTAC 
TCCTGCCTCC CGTGCTCCGA GTCCCTAGAA GGACTCCAGG TGGGTAGGAG CACTGCACAG 
GAAAAAGACC ACAGCCAGCT TAAAGAACTC TATTCAGCTG GGAACCTGAC AGTGCTATCA 
GCCGACCCCC TGCTTCACCA AGACCCAGTT CAGTTAGACT TCCACTTTCG TCTTACCCCC 
CATTCCTCTG CTCATTGGCA CGGCCTTCTG TGTGACCACC AACTCTTCCT GGATATCCCA 
TTTCAGGCCT TGGAGCAAGG CAACCGAGAA AGTTTGACAG CAACACTGGA GTATGTGGAG 
GAGAAAACCA ATGTGGACTC TGTGTTTGTG AACTTCCAAA GCAATCATAA GGACAGAGGT 
GCCCTGCTTC GAGCCTTTAG CTACATGGGC TTTGAGGTGG TCAGACCAGA TCATCCAGCC 
CTCCCTCCCT GGGACAATGT CATCTTCATG GTGTATCCCC TTGAAAGGGA CCTTGGCCAG 
CCTGGCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094488.1
CDS	join(12..269,271..744)
Translation	LPCTRSRPSLYSLSYIKRGKTRNCLYPFWSPYAYYLYCYKYRITLREKMLPCCYRSITYKEQEDLTLRPHCCLPCSCLPYSCLPCSESLEGLQVGRSTAQEKDHSQLKELYSAGNLTVLSADPLLHQDPVQLDFHFRLTPHSSAHWHGLLCDHQLFLDIPFQALEQGNRESLTATLEYVEEKTNVDSVFVNFQSNHKDRGALLRAFSYMGFEVVRPDHPALPPWDNVIFMVYPLERDLGQPGQ

Target ORF information:

RefSeq Version	NM_001101018.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ornithine decarboxylase antizyme 3 (Oaz3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001101018.1

ORF Insert Sequence:

CTGCCTTGTA CCAGGTCCCG CCCCTCTCTC TACTCCCTTT CTTATATTAA GAGGGGAAAA 
ACACGGAACT GCCTCTACCC ATTCTGGTCA CCATACGCCT ATTACCTCTA CTGTTACAAA 
TACCGGATCA CCCTCCGGGA GAAGATGCTG CCTTGTTGTT ACAGAAGCAT CACTTACAAG 
GAACAGGAGG ACCTGACTCT CCGGCCCCAT TGCTGCCTCC CGTGCTCCTG CCTCCCGTAC 
TCCTGCCTCC CGTGCTCCGA GTCCCTAGAA GGACTCCAGG TGGGTAGGAG CACTGCACAG 
GAAAAAGACC ACAGCCAGCT TAAAGAACTC TATTCAGCTG GGAACCTGAC AGTGCTATCA 
GCCGACCCCC TGCTTCACCA AGACCCAGTT CAGTTAGACT TCCACTTTCG TCTTACCCCC 
CATTCCTCTG CTCATTGGCA CGGCCTTCTG TGTGACCACC AACTCTTCCT GGATATCCCA 
TTTCAGGCCT TGGAGCAAGG CAACCGAGAA AGTTTGACAG CAACACTGGA GTATGTGGAG 
GAGAAAACCA ATGTGGACTC TGTGTTTGTG AACTTCCAAA GCAATCATAA GGACAGAGGT 
GCCCTGCTTC GAGCCTTTAG CTACATGGGC TTTGAGGTGG TCAGACCAGA TCATCCAGCC 
CTCCCTCCCT GGGACAATGT CATCTTCATG GTGTATCCCC TTGAAAGGGA CCTTGGCCAG 
CCTGGCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural and degradative aspects of ornithine decarboxylase antizyme inhibitor 2.
FEBS open bio4510-21(2014)
Ramos-Molina B,Lambertos A,Lopez-Contreras AJ,Kasprzak JM,Czerwoniec A,Bujnicki JM,Cremades A,Pe?afiel R

Proteomic analysis of microvesicles from plasma of healthy donors reveals high individual variability.
Journal of proteomics75(12)3574-84(2012 Jun)
Bastos-Amador P,Royo F,Gonzalez E,Conde-Vancells J,Palomo-Diez L,Borras FE,Falcon-Perez JM

Evidence of a role for antizyme and antizyme inhibitor as regulators of human cancer.
Molecular cancer research : MCR9(10)1285-93(2011 Oct)
Olsen RR,Zetter BR

Ornithine decarboxylase antizyme Oaz3 modulates protein phosphatase activity.
The Journal of biological chemistry286(33)29417-27(2011 Aug)
Ruan Y,Cheng M,Ou Y,Oko R,van der Hoorn FA

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballesc? JL,Oliva R

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Oaz3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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