Oaz3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Oaz3
Entrez Gene ID	53814
Full Name	ornithine decarboxylase antizyme 3
Synonyms	AZ3,Az-3,Oaz-t
General protein information	Preferred Names ornithine decarboxylase antizyme 3 Names ornithine decarboxylase antizyme 3 ODC-Az 3 antizyme 3 testis specific ornithine decarboxylase antizyme
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F2.1
Summary	The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamines. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis; thus, completing the auto-regulatory circuit. This gene encodes antizyme 3, the third member of the antizyme family. Like antizymes 1 and 2, antizyme 3 inhibits ODC activity and polyamine uptake; however, it does not stimulate ODC degradation. Also, while antizymes 1 and 2 have broad tissue distribution, expression of antizyme 3 is restricted to haploid germ cells in testis, suggesting a distinct role for this antizyme in spermiogenesis. Antizyme 3 gene knockout studies showed that homozygous mutant male mice were infertile, and indicated the likely role of this antizyme in the formation of a rigid connection between the sperm head and tail during spermatogenesis. This transcript initiates translation from a non-AUG (CUG) codon that is highly conserved among the antizyme 3 orthologs. [provided by RefSeq, Dec 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_016901.3	NP_058597.2	ornithine decarboxylase antizyme 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	OAZ3	NP_001166042.1
	OAZ3	NP_001122102.1
Canis lupus familiaris (dog)	OAZ3	NP_001128423.1
Mus musculus (house mouse)	Oaz3	NP_058597.2
Homo sapiens (human)	OAZ3	NP_057262.2
Bos taurus (cattle)	OAZ3	NP_001166179.1
Rattus norvegicus (Norway rat)	Oaz3	NP_001094488.1

Gene Ontology
Bibliography

Related articles in PubMed

OAZ-t/OAZ3 is essential for rigid connection of sperm tails to heads in mouse.
Tokuhiro K, Isotani A, Yokota S, Yano Y, Oshio S, Hirose M, Wada M, Fujita K, Ogawa Y, Okabe M, Nishimune Y, Tanaka H
PLoS genetics5(11)e1000712(2009 Nov)

Subcellular localization of antizyme inhibitor 2 in mammalian cells: Influence of intrinsic sequences and interaction with antizymes.
L?pez-Contreras AJ, S?nchez-Laorden BL, Ramos-Molina B, de la Morena ME, Cremades A, Pe?afiel R
Journal of cellular biochemistry107(4)732-40(2009 Jul)

Antizyme 3 inhibits polyamine uptake and ornithine decarboxylase (ODC) activity, but does not stimulate ODC degradation.
Snapir Z, Keren-Paz A, Bercovich Z, Kahana C
The Biochemical journal419(1)99-103, 1 p following 103(2009 Apr)

Transcriptomic analysis of polyamine-related genes and polyamine levels in placenta, yolk sac and fetus during the second half of mouse pregnancy.
Lopez-Garcia C, Lopez-Contreras AJ, Cremades A, Castells MT, Pe?afiel R
Placenta30(3)241-9(2009 Mar)

Transient expression analysis of the mouse ornithine decarboxylase antizyme haploid-specific promoter using in vivo electroporation.
Ike A, Ohta H, Onishi M, Iguchi N, Nishimune Y, Nozaki M
FEBS letters559(1-3)159-64(2004 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results indicated that OAZ-t is essential for the formation of a rigid junction between the head and tail during spermatogenesis.
Title: OAZ-t/OAZ3 is essential for rigid connection of sperm tails to heads in mouse.

results define the transcriptional regulatory elements of the OAZt gene promoter; an initiator, a downstream element, and two cyclic adenosine monophosphate-responsive element (CRE)-like sites in the upstream region
Title: Transient expression analysis of the mouse ornithine decarboxylase antizyme haploid-specific promoter using in vivo electroporation.

antizyme expression requires programmed, ribosomal frameshifting
Title: Discovery of a spermatogenesis stage-specific ornithine decarboxylase antizyme: antizyme 3.

The following Oaz3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Oaz3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu83454	NM_016901.3 Latest version!	Mus musculus ornithine decarboxylase antizyme 3 (Oaz3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Oaz3 ( NM_016901.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058597.2 Mus musculus ornithine decarboxylase antizyme 3 (Oaz3), mRNA.

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CloneID	OMu83454
Clone ID Related Accession (Same CDS sequence)	NM_016901.3
Accession Version	NM_016901.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	ornithine decarboxylase antizyme 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC122808.3, AF175297.1 and AI449747.1. On Aug 15, 2014 this sequence version replaced NM_016901.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF175297.1, AK076942.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: inferred from conservation ribosomal slippage :: PMID: 10792465 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGCCTTGTA ACAGGTCCCG CCCCTCTCTC TACTCCCTTT CTTATATCAA GAGGGGAAAA 
ACACGGAACT ATCTCTATCC ATTCTGGTCA CCATTCGCCT ATTACCTCTA CTGTTACAAA 
TACCGGATCA CCCTCCGGGA GAAGATGCTG CCTTGTTGTT ACAAAAGCAT CACTTACAAG 
GAACAGGAGG ACCTGACTCT CCGGCCCCAT TGCTGCCTCC CGTGCTCCTG CCTCCCGTGC 
TCCTGCCTCC AGTGCTCCGA GTCCCTAGGA GGCCTCCAGG TGGGTAGGAG CACTGCACAG 
GAAAAAGACC ACAGCCAGCT TAAAGAACTC TATTCAGCTG GGAACCTGAC AGTGCTATCA 
ACTGACCCCC TGCTTCACCA AGATCCAGTT CAGTTAGACT TCCACTTTCG TCTTACCCCC 
CATTCCTCTG CTCATTGGCA CGGCCTTCTG TGTGATCACC GACTCTTCCT GGATATCCCA 
TATCAGGCCT TGGATCAAGG CAACCGAGAA AGCTTGACAG CAACACTGGA GTATGTGGAG 
GAGAAAACCA ATGTGGACTC TGTGTTTGTG AACTTCCAAA TCGATCGGAA GGACAGAGGT 
GCCCTGCTGC GAGCCTTTAG CTACATGGGC TTCGAGGTGG TTAGACCAGA TCATCCTGCC 
CTCCCTCCCT GGGACAATGT CATCTTCATG GTGTATCCCC TTGAAAGGGA CCTTGGCCAC 
CCTGGCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058597.2
CDS	join(98..355,357..830)
Translation	LPCNRSRPSLYSLSYIKRGKTRNYLYPFWSPFAYYLYCYKYRITLREKMLPCCYKSITYKEQEDLTLRPHCCLPCSCLPCSCLQCSESLGGLQVGRSTAQEKDHSQLKELYSAGNLTVLSTDPLLHQDPVQLDFHFRLTPHSSAHWHGLLCDHRLFLDIPYQALDQGNRESLTATLEYVEEKTNVDSVFVNFQIDRKDRGALLRAFSYMGFEVVRPDHPALPPWDNVIFMVYPLERDLGHPGQ

Target ORF information:

RefSeq Version	NM_016901.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ornithine decarboxylase antizyme 3 (Oaz3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016901.3

ORF Insert Sequence:

CTGCCTTGTA ACAGGTCCCG CCCCTCTCTC TACTCCCTTT CTTATATCAA GAGGGGAAAA 
ACACGGAACT ATCTCTATCC ATTCTGGTCA CCATTCGCCT ATTACCTCTA CTGTTACAAA 
TACCGGATCA CCCTCCGGGA GAAGATGCTG CCTTGTTGTT ACAAAAGCAT CACTTACAAG 
GAACAGGAGG ACCTGACTCT CCGGCCCCAT TGCTGCCTCC CGTGCTCCTG CCTCCCGTGC 
TCCTGCCTCC AGTGCTCCGA GTCCCTAGGA GGCCTCCAGG TGGGTAGGAG CACTGCACAG 
GAAAAAGACC ACAGCCAGCT TAAAGAACTC TATTCAGCTG GGAACCTGAC AGTGCTATCA 
ACTGACCCCC TGCTTCACCA AGATCCAGTT CAGTTAGACT TCCACTTTCG TCTTACCCCC 
CATTCCTCTG CTCATTGGCA CGGCCTTCTG TGTGATCACC GACTCTTCCT GGATATCCCA 
TATCAGGCCT TGGATCAAGG CAACCGAGAA AGCTTGACAG CAACACTGGA GTATGTGGAG 
GAGAAAACCA ATGTGGACTC TGTGTTTGTG AACTTCCAAA TCGATCGGAA GGACAGAGGT 
GCCCTGCTGC GAGCCTTTAG CTACATGGGC TTCGAGGTGG TTAGACCAGA TCATCCTGCC 
CTCCCTCCCT GGGACAATGT CATCTTCATG GTGTATCCCC TTGAAAGGGA CCTTGGCCAC 
CCTGGCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

OAZ-t/OAZ3 is essential for rigid connection of sperm tails to heads in mouse.
PLoS genetics5(11)e1000712(2009 Nov)
Tokuhiro K,Isotani A,Yokota S,Yano Y,Oshio S,Hirose M,Wada M,Fujita K,Ogawa Y,Okabe M,Nishimune Y,Tanaka H

Subcellular localization of antizyme inhibitor 2 in mammalian cells: Influence of intrinsic sequences and interaction with antizymes.
Journal of cellular biochemistry107(4)732-40(2009 Jul)
L?pez-Contreras AJ,S?nchez-Laorden BL,Ramos-Molina B,de la Morena ME,Cremades A,Pe?afiel R

Antizyme 3 inhibits polyamine uptake and ornithine decarboxylase (ODC) activity, but does not stimulate ODC degradation.
The Biochemical journal419(1)99-103, 1 p following 103(2009 Apr)
Snapir Z,Keren-Paz A,Bercovich Z,Kahana C

Transcriptomic analysis of polyamine-related genes and polyamine levels in placenta, yolk sac and fetus during the second half of mouse pregnancy.
Placenta30(3)241-9(2009 Mar)
Lopez-Garcia C,Lopez-Contreras AJ,Cremades A,Castells MT,Pe?afiel R

Transient expression analysis of the mouse ornithine decarboxylase antizyme haploid-specific promoter using in vivo electroporation.
FEBS letters559(1-3)159-64(2004 Feb)
Ike A,Ohta H,Onishi M,Iguchi N,Nishimune Y,Nozaki M

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Oaz3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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