Gpr20 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpr20
Entrez Gene ID	60667
Full Name	G protein-coupled receptor 20
Synonyms	Gpcr5-1,P2Y4
General protein information	Preferred Names G protein-coupled receptor 20 Names G-protein coupled receptor 20 G protein-coupled receptor 5-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q34
Summary	a nucleotide receptor and may be a homolog of the human pyrimidinoceptor [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006241737.3	XP_006241799.1	G-protein coupled receptor 20 isoform X2
NM_022216.1	NP_071552.1	G-protein coupled receptor 20
XM_006241735.3	XP_006241797.1	G-protein coupled receptor 20 isoform X1
XM_017595077.1	XP_017450566.1	G-protein coupled receptor 20 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	GPR20	NP_005284.2
Pan troglodytes (chimpanzee)	GPR20	XP_001148193.1
Bos taurus (cattle)	GPR20	XP_005215361.1
Danio rerio (zebrafish)	LOC794428	XP_001334394.3
	GPR20	XP_002805547.1
Gallus gallus (chicken)	GPR20	XP_425941.2
Canis lupus familiaris (dog)	GPR20	XP_539182.3
Mus musculus (house mouse)	Gpr20	NP_775541.1
Rattus norvegicus (Norway rat)	Gpr20	NP_071552.1
Xenopus tropicalis (tropical clawed frog)	gpr20	XP_002934513.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural basis for endosomal trafficking of diverse transmembrane cargos by PX-FERM proteins.
Ghai R, Bugarcic A, Liu H, Norwood SJ, Skeldal S, Coulson EJ, Li SS, Teasdale RD, Collins BM
Proceedings of the National Academy of Sciences of the United States of America110(8)E643-52(2013 Feb)

Characterization of an orphan G protein-coupled receptor, GPR20, that constitutively activates Gi proteins.
Hase M, Yokomizo T, Shimizu T, Nakamura M
The Journal of biological chemistry283(19)12747-55(2008 May)

Agonist versus antagonist action of ATP at the P2Y4 receptor is determined by the second extracellular loop.
Herold CL, Qi AD, Harden TK, Nicholas RA
The Journal of biological chemistry279(12)11456-64(2004 Mar)

Molecular cloning and characterization of rat P2Y4 nucleotide receptor.
Bogdanov YD, Wildman SS, Clements MP, King BF, Burnstock G
British journal of pharmacology124(3)428-30(1998 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GPR20 constitutively activates G(i) proteins without ligand stimulation
Title: Characterization of an orphan G protein-coupled receptor, GPR20, that constitutively activates Gi proteins.

second extracellular loop and the NH(2) terminus form a functional motif that plays a key role in determining whether ATP functions as an agonist or antagonist at mammalian P2Y(4) receptors
Title: Agonist versus antagonist action of ATP at the P2Y4 receptor is determined by the second extracellular loop.

The following Gpr20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpr20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa41402	NM_022216.1 Latest version!	Rattus norvegicus G protein-coupled receptor 20 (Gpr20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa30425	XM_006241735.3 Latest version!	Rattus norvegicus G protein-coupled receptor 20 (Gpr20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa30426	XM_017595077.1 Latest version!	Rattus norvegicus G protein-coupled receptor 20 (Gpr20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa30426	XM_006241737.3 Latest version!	Rattus norvegicus G protein-coupled receptor 20 (Gpr20), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpr20 ( NM_022216.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071552.1 Rattus norvegicus G protein-coupled receptor 20 (Gpr20), mRNA.

Price & Availability↑

CloneID	ORa41402
Clone ID Related Accession (Same CDS sequence)	NM_022216.1
Accession Version	NM_022216.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Rattus norvegicus(Norway rat)
Product	G-protein coupled receptor 20
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from Y14706.1.

ATGGCCATGC CCTCTGCGTT GTCTTCGAGG CCCTGGGATG CTGTGTTCCC CAATACCACT 
ACAGCAGCAT GGACCAATGG AAGTGTGCCA GAGACTCCCC TGTTCTACCT ATTCGCCCGG 
CTGGATGAGG AACTTCATGC CACCTTCCCC AGCCTGTGGC TGGCACTAAT GGTCGTGCAT 
GGCACCATCT TCCTTGCCGG GCTGTTCCTC AATGGACTGG CGCTGTACGT CTTCTGCTGC 
CGCACGCGGG CCAAGACACC TTCAGTCACC ATATCCATCA ACCTGGTGGT GACTGACCTG 
CTGGTGGGCC TGTCCCTGCC CACACGCTTT GCAGTCTTCT ACGGTGCCCG CGGCTGCCTA 
CACTGTGCCT TCCCTCACGT CCTGGGCTAC TTCCTCAACA TGCACTGTTC TATCCTCTTC 
CTCACCTGCA TCTGTGTGGA CCGCTACCTG GCCATTGTGC AACCTGAGGG CTCCCATCGC 
TGGCGCCAGC CGGCCTGCGC CAAGGCTGTG TGCGTCTTTG TGTGGCTGGC AGCGGGAGTC 
GTGACCCTGT CTGTATTGGG TGTGAAGACT GGTGGACGGT CATGCTGCCG GGTCTTTGCT 
CTGACCGTTC TGGAGTTCCT GCTGCCGCTA CTGGTCATCA GCGTGTTCAC AGGCCGTATC 
ATGTGTGCAC TGTCGAGGCC AGGCCTCGTA CGCCAGGGTC GTCAGCGGCG CATGAGGGCC 
ATGCAGCTGC TGCTCACGGT GCTGGTCATC TTCCTGGTCT GTTTCACACC CTTCCATGCC 
CGCCAGGTGA CTGTGGCACT GTGGCCCAAT GTGCCTTACC ATACCAGCCT TGTGGCTTAC 
CATGTGGCCG TCACCCTCAG CAGCCTCAAC AGCTGCATGG ACCCCATCGT CTACTGTTTT 
ATCACCAGTG GTTTCCAGGC CACTGTCCAA GGCCTCTTCT ACCAGCGTGG AGAAGAATTC 
AAGCCCAGCA CTATGGATAT GGTCAGCATG CACAAGAGCA CCAAGGGCTC AGCCCCATTC 
ACATCCTCAG CACCGGTTCT CACACCCTCA CCCAGCCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071552.1
CDS	1..1062
Translation	MAMPSALSSRPWDAVFPNTTTAAWTNGSVPETPLFYLFARLDEELHATFPSLWLALMVVHGTIFLAGLFLNGLALYVFCCRTRAKTPSVTISINLVVTDLLVGLSLPTRFAVFYGARGCLHCAFPHVLGYFLNMHCSILFLTCICVDRYLAIVQPEGSHRWRQPACAKAVCVFVWLAAGVVTLSVLGVKTGGRSCCRVFALTVLEFLLPLLVISVFTGRIMCALSRPGLVRQGRQRRMRAMQLLLTVLVIFLVCFTPFHARQVTVALWPNVPYHTSLVAYHVAVTLSSLNSCMDPIVYCFITSGFQATVQGLFYQRGEEFKPSTMDMVSMHKSTKGSAPFTSSAPVLTPSPSL

Target ORF information:

RefSeq Version	NM_022216.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein-coupled receptor 20 (Gpr20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022216.1

ORF Insert Sequence:

ATGGCCATGC CCTCTGCGTT GTCTTCGAGG CCCTGGGATG CTGTGTTCCC CAATACCACT 
ACAGCAGCAT GGACCAATGG AAGTGTGCCA GAGACTCCCC TGTTCTACCT ATTCGCCCGG 
CTGGATGAGG AACTTCATGC CACCTTCCCC AGCCTGTGGC TGGCACTAAT GGTCGTGCAT 
GGCACCATCT TCCTTGCCGG GCTGTTCCTC AATGGACTGG CGCTGTACGT CTTCTGCTGC 
CGCACGCGGG CCAAGACACC TTCAGTCACC ATATCCATCA ACCTGGTGGT GACTGACCTG 
CTGGTGGGCC TGTCCCTGCC CACACGCTTT GCAGTCTTCT ACGGTGCCCG CGGCTGCCTA 
CACTGTGCCT TCCCTCACGT CCTGGGCTAC TTCCTCAACA TGCACTGTTC TATCCTCTTC 
CTCACCTGCA TCTGTGTGGA CCGCTACCTG GCCATTGTGC AACCTGAGGG CTCCCATCGC 
TGGCGCCAGC CGGCCTGCGC CAAGGCTGTG TGCGTCTTTG TGTGGCTGGC AGCGGGAGTC 
GTGACCCTGT CTGTATTGGG TGTGAAGACT GGTGGACGGT CATGCTGCCG GGTCTTTGCT 
CTGACCGTTC TGGAGTTCCT GCTGCCGCTA CTGGTCATCA GCGTGTTCAC AGGCCGTATC 
ATGTGTGCAC TGTCGAGGCC AGGCCTCGTA CGCCAGGGTC GTCAGCGGCG CATGAGGGCC 
ATGCAGCTGC TGCTCACGGT GCTGGTCATC TTCCTGGTCT GTTTCACACC CTTCCATGCC 
CGCCAGGTGA CTGTGGCACT GTGGCCCAAT GTGCCTTACC ATACCAGCCT TGTGGCTTAC 
CATGTGGCCG TCACCCTCAG CAGCCTCAAC AGCTGCATGG ACCCCATCGT CTACTGTTTT 
ATCACCAGTG GTTTCCAGGC CACTGTCCAA GGCCTCTTCT ACCAGCGTGG AGAAGAATTC 
AAGCCCAGCA CTATGGATAT GGTCAGCATG CACAAGAGCA CCAAGGGCTC AGCCCCATTC 
ACATCCTCAG CACCGGTTCT CACACCCTCA CCCAGCCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr20 ( XM_006241735.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241797.1 Rattus norvegicus G protein-coupled receptor 20 (Gpr20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa30425
Clone ID Related Accession (Same CDS sequence)	XM_006241735.3
Accession Version	XM_006241735.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	G-protein coupled receptor 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241735.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCAAG ACCCGGCTGG GGGTTCACTG ACCATGGCCA TGCCCTCTGC GTTGTCTTCG 
AGGCCCTGGG ATGCTGTGTT CCCCAATACC ACTACAGCAG CATGGACCAA TGGAAGTGTG 
CCAGAGACTC CCCTGTTCTA CCTATTCGCC CGGCTGGATG AGGAACTTCA TGCCACCTTC 
CCCAGCCTGT GGCTGGCACT AATGGTCGTG CATGGCACCA TCTTCCTTGC CGGGCTGTTC 
CTCAATGGAC TGGCGCTGTA CGTCTTCTGC TGCCGCACGC GGGCCAAGAC ACCTTCAGTC 
ACCTATACCA TCAACCTGGT GGTGACTGAC CTGCTGGTGG GCCTGTCCCT GCCCACACGC 
TTTGCAGTCT TCTACGGTGC CCGCGGCTGC CTACACTGTG CCTTCCCTCA CGTCCTGGGC 
TACTTCCTCA ACATGCACTG TTCTATCCTC TTCCTCACCT GCATCTGTGT GGACCGCTAC 
CTGGCCATTG TGCAACCTGA GGGCTCCCAT CGCTGGCGCC AGCCGGCCTG CGCCAAGGCT 
GTGTGCGTCT TTGTGTGGCT GGCAGCGGGA GTCGTGACCC TGTCTGTATT GGGTGTGAAG 
ACTGGTGGAC GGTCATGCTG CCGGGTCTTT GCTCTGACCG TTCTGGAGTT CCTGCTGCCG 
CTACTGGTCA TCAGCGTGTT CACAGGCCGT ATCATGTGTG CACTGTCGAG GCCAGGCCTG 
CTACGCCAGG GTCGTCAGCG GCGCATGAGG GCCATGCAGC TGCTGCTCAC GGTGCTGGTC 
ATCTTCCTGG TCTGTTTCAC ACCCTTCCAT GCCCGCCAGG TGACTGTGGC ACTGTGGCCC 
AATGTGCCTT ACCATACCAG CCTTGTGGCT TACCATGTGG CCGTCACCCT CAGCAGCCTC 
AACAGCTGCA TGGACCCCAT CGTCTACTGT TTTATCACCA GTGGTTTCCA GGCCACTGTC 
CAAGGCCTCT TCTACCAGCG TGGAGAAGAA TTCAAGCCCA GCACTATGGA TATGGTCAGC 
ATGCACAAGA GCACCAAGGG CTCAGCCCCC ATTCACATCC TCAGCACCGG TTCTCACACC 
CTCACCCAGC CTTTGACCAA CGGGCCTGAG CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241797.1
CDS	70..1185
Translation	MWQDPAGGSLTMAMPSALSSRPWDAVFPNTTTAAWTNGSVPETPLFYLFARLDEELHATFPSLWLALMVVHGTIFLAGLFLNGLALYVFCCRTRAKTPSVTYTINLVVTDLLVGLSLPTRFAVFYGARGCLHCAFPHVLGYFLNMHCSILFLTCICVDRYLAIVQPEGSHRWRQPACAKAVCVFVWLAAGVVTLSVLGVKTGGRSCCRVFALTVLEFLLPLLVISVFTGRIMCALSRPGLLRQGRQRRMRAMQLLLTVLVIFLVCFTPFHARQVTVALWPNVPYHTSLVAYHVAVTLSSLNSCMDPIVYCFITSGFQATVQGLFYQRGEEFKPSTMDMVSMHKSTKGSAPIHILSTGSHTLTQPLTNGPEP

Target ORF information:

RefSeq Version	XM_006241735.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein-coupled receptor 20 (Gpr20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241735.3

ORF Insert Sequence:

ATGTGGCAAG ACCCGGCTGG GGGTTCACTG ACCATGGCCA TGCCCTCTGC GTTGTCTTCG 
AGGCCCTGGG ATGCTGTGTT CCCCAATACC ACTACAGCAG CATGGACCAA TGGAAGTGTG 
CCAGAGACTC CCCTGTTCTA CCTATTCGCC CGGCTGGATG AGGAACTTCA TGCCACCTTC 
CCCAGCCTGT GGCTGGCACT AATGGTCGTG CATGGCACCA TCTTCCTTGC CGGGCTGTTC 
CTCAATGGAC TGGCGCTGTA CGTCTTCTGC TGCCGCACGC GGGCCAAGAC ACCTTCAGTC 
ACCTATACCA TCAACCTGGT GGTGACTGAC CTGCTGGTGG GCCTGTCCCT GCCCACACGC 
TTTGCAGTCT TCTACGGTGC CCGCGGCTGC CTACACTGTG CCTTCCCTCA CGTCCTGGGC 
TACTTCCTCA ACATGCACTG TTCTATCCTC TTCCTCACCT GCATCTGTGT GGACCGCTAC 
CTGGCCATTG TGCAACCTGA GGGCTCCCAT CGCTGGCGCC AGCCGGCCTG CGCCAAGGCT 
GTGTGCGTCT TTGTGTGGCT GGCAGCGGGA GTCGTGACCC TGTCTGTATT GGGTGTGAAG 
ACTGGTGGAC GGTCATGCTG CCGGGTCTTT GCTCTGACCG TTCTGGAGTT CCTGCTGCCG 
CTACTGGTCA TCAGCGTGTT CACAGGCCGT ATCATGTGTG CACTGTCGAG GCCAGGCCTG 
CTACGCCAGG GTCGTCAGCG GCGCATGAGG GCCATGCAGC TGCTGCTCAC GGTGCTGGTC 
ATCTTCCTGG TCTGTTTCAC ACCCTTCCAT GCCCGCCAGG TGACTGTGGC ACTGTGGCCC 
AATGTGCCTT ACCATACCAG CCTTGTGGCT TACCATGTGG CCGTCACCCT CAGCAGCCTC 
AACAGCTGCA TGGACCCCAT CGTCTACTGT TTTATCACCA GTGGTTTCCA GGCCACTGTC 
CAAGGCCTCT TCTACCAGCG TGGAGAAGAA TTCAAGCCCA GCACTATGGA TATGGTCAGC 
ATGCACAAGA GCACCAAGGG CTCAGCCCCC ATTCACATCC TCAGCACCGG TTCTCACACC 
CTCACCCAGC CTTTGACCAA CGGGCCTGAG CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr20 ( XM_006241737.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241799.1 Rattus norvegicus G protein-coupled receptor 20 (Gpr20), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa30426
Clone ID Related Accession (Same CDS sequence)	XM_006241737.3 , XM_017595077.1
Accession Version	XM_006241737.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	G-protein coupled receptor 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241737.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATGC CCTCTGCGTT GTCTTCGAGG CCCTGGGATG CTGTGTTCCC CAATACCACT 
ACAGCAGCAT GGACCAATGG AAGTGTGCCA GAGACTCCCC TGTTCTACCT ATTCGCCCGG 
CTGGATGAGG AACTTCATGC CACCTTCCCC AGCCTGTGGC TGGCACTAAT GGTCGTGCAT 
GGCACCATCT TCCTTGCCGG GCTGTTCCTC AATGGACTGG CGCTGTACGT CTTCTGCTGC 
CGCACGCGGG CCAAGACACC TTCAGTCACC TATACCATCA ACCTGGTGGT GACTGACCTG 
CTGGTGGGCC TGTCCCTGCC CACACGCTTT GCAGTCTTCT ACGGTGCCCG CGGCTGCCTA 
CACTGTGCCT TCCCTCACGT CCTGGGCTAC TTCCTCAACA TGCACTGTTC TATCCTCTTC 
CTCACCTGCA TCTGTGTGGA CCGCTACCTG GCCATTGTGC AACCTGAGGG CTCCCATCGC 
TGGCGCCAGC CGGCCTGCGC CAAGGCTGTG TGCGTCTTTG TGTGGCTGGC AGCGGGAGTC 
GTGACCCTGT CTGTATTGGG TGTGAAGACT GGTGGACGGT CATGCTGCCG GGTCTTTGCT 
CTGACCGTTC TGGAGTTCCT GCTGCCGCTA CTGGTCATCA GCGTGTTCAC AGGCCGTATC 
ATGTGTGCAC TGTCGAGGCC AGGCCTGCTA CGCCAGGGTC GTCAGCGGCG CATGAGGGCC 
ATGCAGCTGC TGCTCACGGT GCTGGTCATC TTCCTGGTCT GTTTCACACC CTTCCATGCC 
CGCCAGGTGA CTGTGGCACT GTGGCCCAAT GTGCCTTACC ATACCAGCCT TGTGGCTTAC 
CATGTGGCCG TCACCCTCAG CAGCCTCAAC AGCTGCATGG ACCCCATCGT CTACTGTTTT 
ATCACCAGTG GTTTCCAGGC CACTGTCCAA GGCCTCTTCT ACCAGCGTGG AGAAGAATTC 
AAGCCCAGCA CTATGGATAT GGTCAGCATG CACAAGAGCA CCAAGGGCTC AGCCCCCATT 
CACATCCTCA GCACCGGTTC TCACACCCTC ACCCAGCCTT TGACCAACGG GCCTGAGCCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241799.1
CDS	284..1366
Translation	MAMPSALSSRPWDAVFPNTTTAAWTNGSVPETPLFYLFARLDEELHATFPSLWLALMVVHGTIFLAGLFLNGLALYVFCCRTRAKTPSVTYTINLVVTDLLVGLSLPTRFAVFYGARGCLHCAFPHVLGYFLNMHCSILFLTCICVDRYLAIVQPEGSHRWRQPACAKAVCVFVWLAAGVVTLSVLGVKTGGRSCCRVFALTVLEFLLPLLVISVFTGRIMCALSRPGLLRQGRQRRMRAMQLLLTVLVIFLVCFTPFHARQVTVALWPNVPYHTSLVAYHVAVTLSSLNSCMDPIVYCFITSGFQATVQGLFYQRGEEFKPSTMDMVSMHKSTKGSAPIHILSTGSHTLTQPLTNGPEP

Target ORF information:

RefSeq Version	XM_006241737.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein-coupled receptor 20 (Gpr20), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241737.3

ORF Insert Sequence:

ATGGCCATGC CCTCTGCGTT GTCTTCGAGG CCCTGGGATG CTGTGTTCCC CAATACCACT 
ACAGCAGCAT GGACCAATGG AAGTGTGCCA GAGACTCCCC TGTTCTACCT ATTCGCCCGG 
CTGGATGAGG AACTTCATGC CACCTTCCCC AGCCTGTGGC TGGCACTAAT GGTCGTGCAT 
GGCACCATCT TCCTTGCCGG GCTGTTCCTC AATGGACTGG CGCTGTACGT CTTCTGCTGC 
CGCACGCGGG CCAAGACACC TTCAGTCACC TATACCATCA ACCTGGTGGT GACTGACCTG 
CTGGTGGGCC TGTCCCTGCC CACACGCTTT GCAGTCTTCT ACGGTGCCCG CGGCTGCCTA 
CACTGTGCCT TCCCTCACGT CCTGGGCTAC TTCCTCAACA TGCACTGTTC TATCCTCTTC 
CTCACCTGCA TCTGTGTGGA CCGCTACCTG GCCATTGTGC AACCTGAGGG CTCCCATCGC 
TGGCGCCAGC CGGCCTGCGC CAAGGCTGTG TGCGTCTTTG TGTGGCTGGC AGCGGGAGTC 
GTGACCCTGT CTGTATTGGG TGTGAAGACT GGTGGACGGT CATGCTGCCG GGTCTTTGCT 
CTGACCGTTC TGGAGTTCCT GCTGCCGCTA CTGGTCATCA GCGTGTTCAC AGGCCGTATC 
ATGTGTGCAC TGTCGAGGCC AGGCCTGCTA CGCCAGGGTC GTCAGCGGCG CATGAGGGCC 
ATGCAGCTGC TGCTCACGGT GCTGGTCATC TTCCTGGTCT GTTTCACACC CTTCCATGCC 
CGCCAGGTGA CTGTGGCACT GTGGCCCAAT GTGCCTTACC ATACCAGCCT TGTGGCTTAC 
CATGTGGCCG TCACCCTCAG CAGCCTCAAC AGCTGCATGG ACCCCATCGT CTACTGTTTT 
ATCACCAGTG GTTTCCAGGC CACTGTCCAA GGCCTCTTCT ACCAGCGTGG AGAAGAATTC 
AAGCCCAGCA CTATGGATAT GGTCAGCATG CACAAGAGCA CCAAGGGCTC AGCCCCCATT 
CACATCCTCA GCACCGGTTC TCACACCCTC ACCCAGCCTT TGACCAACGG GCCTGAGCCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr20 ( XM_017595077.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450566.1 Rattus norvegicus G protein-coupled receptor 20 (Gpr20), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa30426
Clone ID Related Accession (Same CDS sequence)	XM_006241737.3 , XM_017595077.1
Accession Version	XM_017595077.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	G-protein coupled receptor 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATGC CCTCTGCGTT GTCTTCGAGG CCCTGGGATG CTGTGTTCCC CAATACCACT 
ACAGCAGCAT GGACCAATGG AAGTGTGCCA GAGACTCCCC TGTTCTACCT ATTCGCCCGG 
CTGGATGAGG AACTTCATGC CACCTTCCCC AGCCTGTGGC TGGCACTAAT GGTCGTGCAT 
GGCACCATCT TCCTTGCCGG GCTGTTCCTC AATGGACTGG CGCTGTACGT CTTCTGCTGC 
CGCACGCGGG CCAAGACACC TTCAGTCACC TATACCATCA ACCTGGTGGT GACTGACCTG 
CTGGTGGGCC TGTCCCTGCC CACACGCTTT GCAGTCTTCT ACGGTGCCCG CGGCTGCCTA 
CACTGTGCCT TCCCTCACGT CCTGGGCTAC TTCCTCAACA TGCACTGTTC TATCCTCTTC 
CTCACCTGCA TCTGTGTGGA CCGCTACCTG GCCATTGTGC AACCTGAGGG CTCCCATCGC 
TGGCGCCAGC CGGCCTGCGC CAAGGCTGTG TGCGTCTTTG TGTGGCTGGC AGCGGGAGTC 
GTGACCCTGT CTGTATTGGG TGTGAAGACT GGTGGACGGT CATGCTGCCG GGTCTTTGCT 
CTGACCGTTC TGGAGTTCCT GCTGCCGCTA CTGGTCATCA GCGTGTTCAC AGGCCGTATC 
ATGTGTGCAC TGTCGAGGCC AGGCCTGCTA CGCCAGGGTC GTCAGCGGCG CATGAGGGCC 
ATGCAGCTGC TGCTCACGGT GCTGGTCATC TTCCTGGTCT GTTTCACACC CTTCCATGCC 
CGCCAGGTGA CTGTGGCACT GTGGCCCAAT GTGCCTTACC ATACCAGCCT TGTGGCTTAC 
CATGTGGCCG TCACCCTCAG CAGCCTCAAC AGCTGCATGG ACCCCATCGT CTACTGTTTT 
ATCACCAGTG GTTTCCAGGC CACTGTCCAA GGCCTCTTCT ACCAGCGTGG AGAAGAATTC 
AAGCCCAGCA CTATGGATAT GGTCAGCATG CACAAGAGCA CCAAGGGCTC AGCCCCCATT 
CACATCCTCA GCACCGGTTC TCACACCCTC ACCCAGCCTT TGACCAACGG GCCTGAGCCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450566.1
CDS	235..1317
Translation	MAMPSALSSRPWDAVFPNTTTAAWTNGSVPETPLFYLFARLDEELHATFPSLWLALMVVHGTIFLAGLFLNGLALYVFCCRTRAKTPSVTYTINLVVTDLLVGLSLPTRFAVFYGARGCLHCAFPHVLGYFLNMHCSILFLTCICVDRYLAIVQPEGSHRWRQPACAKAVCVFVWLAAGVVTLSVLGVKTGGRSCCRVFALTVLEFLLPLLVISVFTGRIMCALSRPGLLRQGRQRRMRAMQLLLTVLVIFLVCFTPFHARQVTVALWPNVPYHTSLVAYHVAVTLSSLNSCMDPIVYCFITSGFQATVQGLFYQRGEEFKPSTMDMVSMHKSTKGSAPIHILSTGSHTLTQPLTNGPEP

Target ORF information:

RefSeq Version	XM_017595077.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein-coupled receptor 20 (Gpr20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595077.1

ORF Insert Sequence:

ATGGCCATGC CCTCTGCGTT GTCTTCGAGG CCCTGGGATG CTGTGTTCCC CAATACCACT 
ACAGCAGCAT GGACCAATGG AAGTGTGCCA GAGACTCCCC TGTTCTACCT ATTCGCCCGG 
CTGGATGAGG AACTTCATGC CACCTTCCCC AGCCTGTGGC TGGCACTAAT GGTCGTGCAT 
GGCACCATCT TCCTTGCCGG GCTGTTCCTC AATGGACTGG CGCTGTACGT CTTCTGCTGC 
CGCACGCGGG CCAAGACACC TTCAGTCACC TATACCATCA ACCTGGTGGT GACTGACCTG 
CTGGTGGGCC TGTCCCTGCC CACACGCTTT GCAGTCTTCT ACGGTGCCCG CGGCTGCCTA 
CACTGTGCCT TCCCTCACGT CCTGGGCTAC TTCCTCAACA TGCACTGTTC TATCCTCTTC 
CTCACCTGCA TCTGTGTGGA CCGCTACCTG GCCATTGTGC AACCTGAGGG CTCCCATCGC 
TGGCGCCAGC CGGCCTGCGC CAAGGCTGTG TGCGTCTTTG TGTGGCTGGC AGCGGGAGTC 
GTGACCCTGT CTGTATTGGG TGTGAAGACT GGTGGACGGT CATGCTGCCG GGTCTTTGCT 
CTGACCGTTC TGGAGTTCCT GCTGCCGCTA CTGGTCATCA GCGTGTTCAC AGGCCGTATC 
ATGTGTGCAC TGTCGAGGCC AGGCCTGCTA CGCCAGGGTC GTCAGCGGCG CATGAGGGCC 
ATGCAGCTGC TGCTCACGGT GCTGGTCATC TTCCTGGTCT GTTTCACACC CTTCCATGCC 
CGCCAGGTGA CTGTGGCACT GTGGCCCAAT GTGCCTTACC ATACCAGCCT TGTGGCTTAC 
CATGTGGCCG TCACCCTCAG CAGCCTCAAC AGCTGCATGG ACCCCATCGT CTACTGTTTT 
ATCACCAGTG GTTTCCAGGC CACTGTCCAA GGCCTCTTCT ACCAGCGTGG AGAAGAATTC 
AAGCCCAGCA CTATGGATAT GGTCAGCATG CACAAGAGCA CCAAGGGCTC AGCCCCCATT 
CACATCCTCA GCACCGGTTC TCACACCCTC ACCCAGCCTT TGACCAACGG GCCTGAGCCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural basis for endosomal trafficking of diverse transmembrane cargos by PX-FERM proteins.
Proceedings of the National Academy of Sciences of the United States of America110(8)E643-52(2013 Feb)
Ghai R,Bugarcic A,Liu H,Norwood SJ,Skeldal S,Coulson EJ,Li SS,Teasdale RD,Collins BM

Characterization of an orphan G protein-coupled receptor, GPR20, that constitutively activates Gi proteins.
The Journal of biological chemistry283(19)12747-55(2008 May)
Hase M,Yokomizo T,Shimizu T,Nakamura M

Agonist versus antagonist action of ATP at the P2Y4 receptor is determined by the second extracellular loop.
The Journal of biological chemistry279(12)11456-64(2004 Mar)
Herold CL,Qi AD,Harden TK,Nicholas RA

Molecular cloning and characterization of rat P2Y4 nucleotide receptor.
British journal of pharmacology124(3)428-30(1998 Jun)
Bogdanov YD,Wildman SS,Clements MP,King BF,Burnstock G

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Gpr20 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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