Gpr20 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpr20
Entrez Gene ID	239530
Full Name	G protein-coupled receptor 20
Synonyms	A430106B11Rik
General protein information	Preferred Names G protein-coupled receptor 20 Names G-protein coupled receptor 20 G protein-coupled receptor GPR20
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_173365.2	NP_775541.1	G-protein coupled receptor 20
XM_006520927.1	XP_006520990.1	G-protein coupled receptor 20 isoform X1
XM_006520927.2	XP_006520990.1	G-protein coupled receptor 20 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	GPR20	NP_005284.2
Pan troglodytes (chimpanzee)	GPR20	XP_001148193.1
Bos taurus (cattle)	GPR20	XP_005215361.1
Danio rerio (zebrafish)	LOC794428	XP_001334394.3
	GPR20	XP_002805547.1
Gallus gallus (chicken)	GPR20	XP_425941.2
Canis lupus familiaris (dog)	GPR20	XP_539182.3
Mus musculus (house mouse)	Gpr20	NP_775541.1
Rattus norvegicus (Norway rat)	Gpr20	NP_071552.1
Xenopus tropicalis (tropical clawed frog)	gpr20	XP_002934513.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Transcriptome of the inner circular smooth muscle of the developing mouse intestine: Evidence for regulation of visceral smooth muscle genes by the hedgehog target gene, cJun.
Gurdziel K, Vogt KR, Walton KD, Schneider GK, Gumucio DL
Developmental dynamics : an official publication of the American Association of Anatomists245(5)614-26(2016 May)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Structural basis for endosomal trafficking of diverse transmembrane cargos by PX-FERM proteins.
Ghai R, Bugarcic A, Liu H, Norwood SJ, Skeldal S, Coulson EJ, Li SS, Teasdale RD, Collins BM
Proceedings of the National Academy of Sciences of the United States of America110(8)E643-52(2013 Feb)

The G protein-coupled receptor repertoires of human and mouse.
Vassilatis DK, Hohmann JG, Zeng H, Li F, Ranchalis JE, Mortrud MT, Brown A, Rodriguez SS, Weller JR, Wright AC, Bergmann JE, Gaitanaris GA
Proceedings of the National Academy of Sciences of the United States of America100(8)4903-8(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gpr20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpr20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu04105	NM_173365.2 Latest version!	Mus musculus G protein-coupled receptor 20 (Gpr20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu04105	XM_006520927.2 Latest version!	Mus musculus G protein-coupled receptor 20 (Gpr20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
View Old Accession Versions >>
OMu04105	XM_006520927.1	Mus musculus G protein-coupled receptor 20 (Gpr20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpr20 ( NM_173365.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_775541.1 Mus musculus G protein-coupled receptor 20 (Gpr20), mRNA.

Price & Availability↑

CloneID	OMu04105
Clone ID Related Accession (Same CDS sequence)	NM_173365.2 , XM_006520927.1 , XM_006520927.2
Accession Version	NM_173365.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	G-protein coupled receptor 20
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK040547.1. On Apr 6, 2007 this sequence version replaced NM_173365.1. ##Evidence-Data-START## Transcript exon combination :: AK040547.1, BC117783.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCCCTCTG CGTTGTCTAT GAGGCCCTGG GATGCTGCAC TCCCCAACAC CACTGCAGCA 
GCATGGACCA ATGGAAGTGT GCCGGAGATG CCCCTGTTCC ATCACTTTGC CCGGCTGGAT 
GAGGAACTGC AAGCCACCTT CCCCAGCCTG TGGCAAGCAC TAATGGTCGT GCACGGCACC 
ATCTTCCTTG CCGGGCTGGT GCTCAACGGA CTGGCACTGT ATGTCTTCTG CTGTCGCACG 
CGGGCCAAGA CCCCTTCAGT CACCTATACC ATCAACCTGG TGGTGACTGA CCTGCTAGTG 
GGCCTGTCCC TGCCCACACG CTTTGCTGTC TTCTACGGTG CCCGAGGCTG TCTGCGCTGT 
GCCTTCCCTC ATGTCCTGGG CTACTTCCTC AACATGCACT GTTCCATCCT CTTCCTCACC 
TGCATCTGCG TGGACCGCTA CCTGGCCATT GTGCAACCCG AGGGTTCCCG CCGCTGGCGC 
CAGCCGGCCT GCGCCAAGGC CGTGTGCATC TTTGTGTGGC TGGCAGCAGG AGTCGTGACC 
CTGTCTGTGC TGGGTGTGAA GTCGGGTGGA CGATCATGCT GCCGTGTCTT TGCTCTGACC 
GTTCTGGAGT TCCTGCTGCC TCTGCTGGTC ATCAGCGTGT TCACAGGCCG GATCATGTGT 
GCGCTGTCGA GGCCGGGTCT GCTACGCCAG GGCCGTCAGC GGCGCGTGAG GGCCATGCAG 
CTGCTGCTCA CGGTGCTGGT CATCTTCCTG GTCTGTTTCA CACCCTTCCA TGCCCGCCAG 
GTGGCTGTGG CACTGTGGCC TAACGTACCT AAGCATACCA GCCTTGTGGC TTACCACGTG 
GCTGTCACCC TCAGCAGCCT CAACAGCTGC ATGGACCCCA TTGTCTACTG TTTCATCACC 
AGTGGCTTCC AGGCCACTGT CCGAGGCCTC TTCTACCAGC GTGGAGAAGA ATTCAAGCCC 
AGCAGTATGG ATGTGGTCAG CATGCACAAG AGCACCAAGG CCTCAGCTCC CATTCACATC 
CTCAGCATCG GTTCCCACAC CCTTACCCAG CCTTTGACCA ACGGGCCTGA GCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775541.1
CDS	210..1286
Translation	MPSALSMRPWDAALPNTTAAAWTNGSVPEMPLFHHFARLDEELQATFPSLWQALMVVHGTIFLAGLVLNGLALYVFCCRTRAKTPSVTYTINLVVTDLLVGLSLPTRFAVFYGARGCLRCAFPHVLGYFLNMHCSILFLTCICVDRYLAIVQPEGSRRWRQPACAKAVCIFVWLAAGVVTLSVLGVKSGGRSCCRVFALTVLEFLLPLLVISVFTGRIMCALSRPGLLRQGRQRRVRAMQLLLTVLVIFLVCFTPFHARQVAVALWPNVPKHTSLVAYHVAVTLSSLNSCMDPIVYCFITSGFQATVRGLFYQRGEEFKPSSMDVVSMHKSTKASAPIHILSIGSHTLTQPLTNGPEP

Target ORF information:

RefSeq Version	NM_173365.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor 20 (Gpr20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173365.2

ORF Insert Sequence:

ATGCCCTCTG CGTTGTCTAT GAGGCCCTGG GATGCTGCAC TCCCCAACAC CACTGCAGCA 
GCATGGACCA ATGGAAGTGT GCCGGAGATG CCCCTGTTCC ATCACTTTGC CCGGCTGGAT 
GAGGAACTGC AAGCCACCTT CCCCAGCCTG TGGCAAGCAC TAATGGTCGT GCACGGCACC 
ATCTTCCTTG CCGGGCTGGT GCTCAACGGA CTGGCACTGT ATGTCTTCTG CTGTCGCACG 
CGGGCCAAGA CCCCTTCAGT CACCTATACC ATCAACCTGG TGGTGACTGA CCTGCTAGTG 
GGCCTGTCCC TGCCCACACG CTTTGCTGTC TTCTACGGTG CCCGAGGCTG TCTGCGCTGT 
GCCTTCCCTC ATGTCCTGGG CTACTTCCTC AACATGCACT GTTCCATCCT CTTCCTCACC 
TGCATCTGCG TGGACCGCTA CCTGGCCATT GTGCAACCCG AGGGTTCCCG CCGCTGGCGC 
CAGCCGGCCT GCGCCAAGGC CGTGTGCATC TTTGTGTGGC TGGCAGCAGG AGTCGTGACC 
CTGTCTGTGC TGGGTGTGAA GTCGGGTGGA CGATCATGCT GCCGTGTCTT TGCTCTGACC 
GTTCTGGAGT TCCTGCTGCC TCTGCTGGTC ATCAGCGTGT TCACAGGCCG GATCATGTGT 
GCGCTGTCGA GGCCGGGTCT GCTACGCCAG GGCCGTCAGC GGCGCGTGAG GGCCATGCAG 
CTGCTGCTCA CGGTGCTGGT CATCTTCCTG GTCTGTTTCA CACCCTTCCA TGCCCGCCAG 
GTGGCTGTGG CACTGTGGCC TAACGTACCT AAGCATACCA GCCTTGTGGC TTACCACGTG 
GCTGTCACCC TCAGCAGCCT CAACAGCTGC ATGGACCCCA TTGTCTACTG TTTCATCACC 
AGTGGCTTCC AGGCCACTGT CCGAGGCCTC TTCTACCAGC GTGGAGAAGA ATTCAAGCCC 
AGCAGTATGG ATGTGGTCAG CATGCACAAG AGCACCAAGG CCTCAGCTCC CATTCACATC 
CTCAGCATCG GTTCCCACAC CCTTACCCAG CCTTTGACCA ACGGGCCTGA GCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr20 ( XM_006520927.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520990.1 Mus musculus G protein-coupled receptor 20 (Gpr20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu04105
Clone ID Related Accession (Same CDS sequence)	NM_173365.2 , XM_006520927.1 , XM_006520927.2
Accession Version	XM_006520927.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	G-protein coupled receptor 20 isoform X1
Comment

ATGCCCTCTG CGTTGTCTAT GAGGCCCTGG GATGCTGCAC TCCCCAACAC CACTGCAGCA 
GCATGGACCA ATGGAAGTGT GCCGGAGATG CCCCTGTTCC ATCACTTTGC CCGGCTGGAT 
GAGGAACTGC AAGCCACCTT CCCCAGCCTG TGGCAAGCAC TAATGGTCGT GCACGGCACC 
ATCTTCCTTG CCGGGCTGGT GCTCAACGGA CTGGCACTGT ATGTCTTCTG CTGTCGCACG 
CGGGCCAAGA CCCCTTCAGT CACCTATACC ATCAACCTGG TGGTGACTGA CCTGCTAGTG 
GGCCTGTCCC TGCCCACACG CTTTGCTGTC TTCTACGGTG CCCGAGGCTG TCTGCGCTGT 
GCCTTCCCTC ATGTCCTGGG CTACTTCCTC AACATGCACT GTTCCATCCT CTTCCTCACC 
TGCATCTGCG TGGACCGCTA CCTGGCCATT GTGCAACCCG AGGGTTCCCG CCGCTGGCGC 
CAGCCGGCCT GCGCCAAGGC CGTGTGCATC TTTGTGTGGC TGGCAGCAGG AGTCGTGACC 
CTGTCTGTGC TGGGTGTGAA GTCGGGTGGA CGATCATGCT GCCGTGTCTT TGCTCTGACC 
GTTCTGGAGT TCCTGCTGCC TCTGCTGGTC ATCAGCGTGT TCACAGGCCG GATCATGTGT 
GCGCTGTCGA GGCCGGGTCT GCTACGCCAG GGCCGTCAGC GGCGCGTGAG GGCCATGCAG 
CTGCTGCTCA CGGTGCTGGT CATCTTCCTG GTCTGTTTCA CACCCTTCCA TGCCCGCCAG 
GTGGCTGTGG CACTGTGGCC TAACGTACCT AAGCATACCA GCCTTGTGGC TTACCACGTG 
GCTGTCACCC TCAGCAGCCT CAACAGCTGC ATGGACCCCA TTGTCTACTG TTTCATCACC 
AGTGGCTTCC AGGCCACTGT CCGAGGCCTC TTCTACCAGC GTGGAGAAGA ATTCAAGCCC 
AGCAGTATGG ATGTGGTCAG CATGCACAAG AGCACCAAGG CCTCAGCTCC CATTCACATC 
CTCAGCATCG GTTCCCACAC CCTTACCCAG CCTTTGACCA ACGGGCCTGA GCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520990.1
Translation	MPSALSMRPWDAALPNTTAAAWTNGSVPEMPLFHHFARLDEELQATFPSLWQALMVVHGTIFLAGLVLNGLALYVFCCRTRAKTPSVTYTINLVVTDLLVGLSLPTRFAVFYGARGCLRCAFPHVLGYFLNMHCSILFLTCICVDRYLAIVQPEGSRRWRQPACAKAVCIFVWLAAGVVTLSVLGVKSGGRSCCRVFALTVLEFLLPLLVISVFTGRIMCALSRPGLLRQGRQRRVRAMQLLLTVLVIFLVCFTPFHARQVAVALWPNVPKHTSLVAYHVAVTLSSLNSCMDPIVYCFITSGFQATVRGLFYQRGEEFKPSSMDVVSMHKSTKASAPIHILSIGSHTLTQPLTNGPEP

Target ORF information:

RefSeq Version	XM_006520927.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus G protein-coupled receptor 20 (Gpr20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520927.1

ORF Insert Sequence:

ATGCCCTCTG CGTTGTCTAT GAGGCCCTGG GATGCTGCAC TCCCCAACAC CACTGCAGCA 
GCATGGACCA ATGGAAGTGT GCCGGAGATG CCCCTGTTCC ATCACTTTGC CCGGCTGGAT 
GAGGAACTGC AAGCCACCTT CCCCAGCCTG TGGCAAGCAC TAATGGTCGT GCACGGCACC 
ATCTTCCTTG CCGGGCTGGT GCTCAACGGA CTGGCACTGT ATGTCTTCTG CTGTCGCACG 
CGGGCCAAGA CCCCTTCAGT CACCTATACC ATCAACCTGG TGGTGACTGA CCTGCTAGTG 
GGCCTGTCCC TGCCCACACG CTTTGCTGTC TTCTACGGTG CCCGAGGCTG TCTGCGCTGT 
GCCTTCCCTC ATGTCCTGGG CTACTTCCTC AACATGCACT GTTCCATCCT CTTCCTCACC 
TGCATCTGCG TGGACCGCTA CCTGGCCATT GTGCAACCCG AGGGTTCCCG CCGCTGGCGC 
CAGCCGGCCT GCGCCAAGGC CGTGTGCATC TTTGTGTGGC TGGCAGCAGG AGTCGTGACC 
CTGTCTGTGC TGGGTGTGAA GTCGGGTGGA CGATCATGCT GCCGTGTCTT TGCTCTGACC 
GTTCTGGAGT TCCTGCTGCC TCTGCTGGTC ATCAGCGTGT TCACAGGCCG GATCATGTGT 
GCGCTGTCGA GGCCGGGTCT GCTACGCCAG GGCCGTCAGC GGCGCGTGAG GGCCATGCAG 
CTGCTGCTCA CGGTGCTGGT CATCTTCCTG GTCTGTTTCA CACCCTTCCA TGCCCGCCAG 
GTGGCTGTGG CACTGTGGCC TAACGTACCT AAGCATACCA GCCTTGTGGC TTACCACGTG 
GCTGTCACCC TCAGCAGCCT CAACAGCTGC ATGGACCCCA TTGTCTACTG TTTCATCACC 
AGTGGCTTCC AGGCCACTGT CCGAGGCCTC TTCTACCAGC GTGGAGAAGA ATTCAAGCCC 
AGCAGTATGG ATGTGGTCAG CATGCACAAG AGCACCAAGG CCTCAGCTCC CATTCACATC 
CTCAGCATCG GTTCCCACAC CCTTACCCAG CCTTTGACCA ACGGGCCTGA GCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr20 ( XM_006520927.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520990.1 Mus musculus G protein-coupled receptor 20 (Gpr20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu04105
Clone ID Related Accession (Same CDS sequence)	NM_173365.2 , XM_006520927.1 , XM_006520927.2
Accession Version	XM_006520927.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	G-protein coupled receptor 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520927.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCTG CGTTGTCTAT GAGGCCCTGG GATGCTGCAC TCCCCAACAC CACTGCAGCA 
GCATGGACCA ATGGAAGTGT GCCGGAGATG CCCCTGTTCC ATCACTTTGC CCGGCTGGAT 
GAGGAACTGC AAGCCACCTT CCCCAGCCTG TGGCAAGCAC TAATGGTCGT GCACGGCACC 
ATCTTCCTTG CCGGGCTGGT GCTCAACGGA CTGGCACTGT ATGTCTTCTG CTGTCGCACG 
CGGGCCAAGA CCCCTTCAGT CACCTATACC ATCAACCTGG TGGTGACTGA CCTGCTAGTG 
GGCCTGTCCC TGCCCACACG CTTTGCTGTC TTCTACGGTG CCCGAGGCTG TCTGCGCTGT 
GCCTTCCCTC ATGTCCTGGG CTACTTCCTC AACATGCACT GTTCCATCCT CTTCCTCACC 
TGCATCTGCG TGGACCGCTA CCTGGCCATT GTGCAACCCG AGGGTTCCCG CCGCTGGCGC 
CAGCCGGCCT GCGCCAAGGC CGTGTGCATC TTTGTGTGGC TGGCAGCAGG AGTCGTGACC 
CTGTCTGTGC TGGGTGTGAA GTCGGGTGGA CGATCATGCT GCCGTGTCTT TGCTCTGACC 
GTTCTGGAGT TCCTGCTGCC TCTGCTGGTC ATCAGCGTGT TCACAGGCCG GATCATGTGT 
GCGCTGTCGA GGCCGGGTCT GCTACGCCAG GGCCGTCAGC GGCGCGTGAG GGCCATGCAG 
CTGCTGCTCA CGGTGCTGGT CATCTTCCTG GTCTGTTTCA CACCCTTCCA TGCCCGCCAG 
GTGGCTGTGG CACTGTGGCC TAACGTACCT AAGCATACCA GCCTTGTGGC TTACCACGTG 
GCTGTCACCC TCAGCAGCCT CAACAGCTGC ATGGACCCCA TTGTCTACTG TTTCATCACC 
AGTGGCTTCC AGGCCACTGT CCGAGGCCTC TTCTACCAGC GTGGAGAAGA ATTCAAGCCC 
AGCAGTATGG ATGTGGTCAG CATGCACAAG AGCACCAAGG CCTCAGCTCC CATTCACATC 
CTCAGCATCG GTTCCCACAC CCTTACCCAG CCTTTGACCA ACGGGCCTGA GCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520990.1
CDS	43..1119
Translation	MPSALSMRPWDAALPNTTAAAWTNGSVPEMPLFHHFARLDEELQATFPSLWQALMVVHGTIFLAGLVLNGLALYVFCCRTRAKTPSVTYTINLVVTDLLVGLSLPTRFAVFYGARGCLRCAFPHVLGYFLNMHCSILFLTCICVDRYLAIVQPEGSRRWRQPACAKAVCIFVWLAAGVVTLSVLGVKSGGRSCCRVFALTVLEFLLPLLVISVFTGRIMCALSRPGLLRQGRQRRVRAMQLLLTVLVIFLVCFTPFHARQVAVALWPNVPKHTSLVAYHVAVTLSSLNSCMDPIVYCFITSGFQATVRGLFYQRGEEFKPSSMDVVSMHKSTKASAPIHILSIGSHTLTQPLTNGPEP

Target ORF information:

RefSeq Version	XM_006520927.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor 20 (Gpr20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520927.2

ORF Insert Sequence:

ATGCCCTCTG CGTTGTCTAT GAGGCCCTGG GATGCTGCAC TCCCCAACAC CACTGCAGCA 
GCATGGACCA ATGGAAGTGT GCCGGAGATG CCCCTGTTCC ATCACTTTGC CCGGCTGGAT 
GAGGAACTGC AAGCCACCTT CCCCAGCCTG TGGCAAGCAC TAATGGTCGT GCACGGCACC 
ATCTTCCTTG CCGGGCTGGT GCTCAACGGA CTGGCACTGT ATGTCTTCTG CTGTCGCACG 
CGGGCCAAGA CCCCTTCAGT CACCTATACC ATCAACCTGG TGGTGACTGA CCTGCTAGTG 
GGCCTGTCCC TGCCCACACG CTTTGCTGTC TTCTACGGTG CCCGAGGCTG TCTGCGCTGT 
GCCTTCCCTC ATGTCCTGGG CTACTTCCTC AACATGCACT GTTCCATCCT CTTCCTCACC 
TGCATCTGCG TGGACCGCTA CCTGGCCATT GTGCAACCCG AGGGTTCCCG CCGCTGGCGC 
CAGCCGGCCT GCGCCAAGGC CGTGTGCATC TTTGTGTGGC TGGCAGCAGG AGTCGTGACC 
CTGTCTGTGC TGGGTGTGAA GTCGGGTGGA CGATCATGCT GCCGTGTCTT TGCTCTGACC 
GTTCTGGAGT TCCTGCTGCC TCTGCTGGTC ATCAGCGTGT TCACAGGCCG GATCATGTGT 
GCGCTGTCGA GGCCGGGTCT GCTACGCCAG GGCCGTCAGC GGCGCGTGAG GGCCATGCAG 
CTGCTGCTCA CGGTGCTGGT CATCTTCCTG GTCTGTTTCA CACCCTTCCA TGCCCGCCAG 
GTGGCTGTGG CACTGTGGCC TAACGTACCT AAGCATACCA GCCTTGTGGC TTACCACGTG 
GCTGTCACCC TCAGCAGCCT CAACAGCTGC ATGGACCCCA TTGTCTACTG TTTCATCACC 
AGTGGCTTCC AGGCCACTGT CCGAGGCCTC TTCTACCAGC GTGGAGAAGA ATTCAAGCCC 
AGCAGTATGG ATGTGGTCAG CATGCACAAG AGCACCAAGG CCTCAGCTCC CATTCACATC 
CTCAGCATCG GTTCCCACAC CCTTACCCAG CCTTTGACCA ACGGGCCTGA GCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Transcriptome of the inner circular smooth muscle of the developing mouse intestine: Evidence for regulation of visceral smooth muscle genes by the hedgehog target gene, cJun.
Developmental dynamics : an official publication of the American Association of Anatomists245(5)614-26(2016 May)
Gurdziel K,Vogt KR,Walton KD,Schneider GK,Gumucio DL

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Structural basis for endosomal trafficking of diverse transmembrane cargos by PX-FERM proteins.
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The G protein-coupled receptor repertoires of human and mouse.
Proceedings of the National Academy of Sciences of the United States of America100(8)4903-8(2003 Apr)
Vassilatis DK,Hohmann JG,Zeng H,Li F,Ranchalis JE,Mortrud MT,Brown A,Rodriguez SS,Weller JR,Wright AC,Bergmann JE,Gaitanaris GA

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Gpr20 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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