Dusp4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dusp4
Entrez Gene ID	60587
Full Name	dual specificity phosphatase 4
Synonyms	Mkp-2,Mkp2
General protein information	Preferred Names dual specificity phosphatase 4 Names dual specificity protein phosphatase 4 MAP kinase phosphatase 2 mitogen-activated protein kinase phosphatase 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16q12.2
Summary	exhibits vanadate-sensitive phosphatase activity for MAP kinase; inhibits transcription of MAP kinase-dependent genes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022199.1	NP_071535.1	dual specificity protein phosphatase 4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Dusp4	NP_071535.1
Canis lupus familiaris (dog)	LOC482880	XP_539995.3
Oryza sativa (rice)	Os02g0720300	NP_001047951.1
	DUSP4	XP_001110903.1
Arabidopsis thaliana (thale cress)	IBR5	NP_178534.2
Oryza sativa (rice)	Os06g0308100	NP_001057473.1
Xenopus tropicalis (tropical clawed frog)	dusp4	NP_001016109.1
Pan troglodytes (chimpanzee)	DUSP4	XP_520046.2
Bos taurus (cattle)	DUSP4	NP_001193882.1
Homo sapiens (human)	DUSP4	NP_001385.1
Mus musculus (house mouse)	Dusp4	NP_795907.1
Gallus gallus (chicken)	DUSP4	NP_990169.1
Danio rerio (zebrafish)	dusp4	NP_957465.1

Gene Ontology
Bibliography

Related articles in PubMed

Redox activation of DUSP4 by N-acetylcysteine protects endothelial cells from Cd??-induced apoptosis.
Barajas-Espinosa A, Basye A, Jesse E, Yan H, Quan D, Chen CA
Free radical biology & medicine74188-199(2014 Sep)

The family-wide structure and function of human dual-specificity protein phosphatases.
Jeong DG, Wei CH, Ku B, Jeon TJ, Chien PN, Kim JK, Park SY, Hwang HS, Ryu SY, Park H, Kim DS, Kim SJ, Ryu SE
Acta crystallographica. Section D, Biological crystallography70(Pt 2)421-35(2014 Feb)

Inhibition of gluconeogenesis through transcriptional activation of EGR1 and DUSP4 by AMP-activated kinase.
Berasi SP, Huard C, Li D, Shih HH, Sun Y, Zhong W, Paulsen JE, Brown EL, Gimeno RE, Martinez RV
The Journal of biological chemistry281(37)27167-77(2006 Sep)

The I(1)-imidazoline receptor in PC12 pheochromocytoma cells reverses NGF-induced ERK activation and induces MKP-2 phosphatase.
Edwards L, Ernsberger P
Brain research980(1)71-9(2003 Aug)

Inhibition of protein tyrosine/mitogen-activated protein kinase phosphatase activity is associated with D2 dopamine receptor supersensitivity in a rat model of Parkinson's disease.
Zhen X, Torres C, Cai G, Friedman E
Molecular pharmacology62(6)1356-63(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DUSP4 gene knockdown further supports the hypothesis that DUSP4 is an antioxidant gene, critical in the modulation of eNOS expression, and thus protects against Cd(2+)-induced stress.
Title: Redox activation of DUSP4 by N-acetylcysteine protects endothelial cells from Cd??-induced apoptosis.

the inhibition of hepatic gluconeogenesis by AMPK may, in part, be mediated by an immediate early gene response involving EGR1 and its target, DUSP4
Title: Inhibition of gluconeogenesis through transcriptional activation of EGR1 and DUSP4 by AMP-activated kinase.

The I(1)-imidazoline receptor in PC12 pheochromocytoma cells induces MKP-2 phosphatase.
Title: The I(1)-imidazoline receptor in PC12 pheochromocytoma cells reverses NGF-induced ERK activation and induces MKP-2 phosphatase.

The following Dusp4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dusp4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05421	NM_022199.1 Latest version!	Rattus norvegicus dual specificity phosphatase 4 (Dusp4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dusp4 ( NM_022199.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071535.1 Rattus norvegicus dual specificity phosphatase 4 (Dusp4), mRNA.

Price & Availability↑

CloneID	ORa05421
Clone ID Related Accession (Same CDS sequence)	NM_022199.1
Accession Version	NM_022199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	dual specificity protein phosphatase 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U23438.1. ##Evidence-Data-START## Transcript exon combination :: U23438.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTGACGA TGGAGGAACT GCGGGAGATG GACTGCAGCG TGCTCAAAAG GCTGATGAAC 
CGAGATGAGA ACGGCGGCAC GGCGGGCAGC AGCGGCGGCA GCCACGGCGC CCTGGGGCTG 
CTGAGCGGCG GCAAGTGCTT GCTGCTGGAC TGCAGGCCGT TTCTGGCTCA CAGCGCGGGC 
TACATCCGAG GCTCGGTGAA CGTGCGCTGC AATACCATCG TGCGGCGGAG GGCCAAGGGC 
TCCGTGAGCC TGGAGCAGAT TCTGCCCGCC GAGGAAGAGG TGCGCGCCCG CCTGCGCTCT 
GGCCTCTACT CGGCTGTCAT CGTCTACGAT GAGCGCAGCC CGCGCGCCGA GAGTCTCCGG 
GAGGACAGCA CAGTGTCGCT GGTCGTGCAG GCGTTGCGCC GGAACGCGGA GCGCACAGAC 
ATCTGCCTGC TTAAAGGTGG CTATGAGAGG TTTTCTTCTG AGTACCCAGA ATTCTGCTCT 
AAAACTAAGG CCCTGGCCGC CATCCCACCC CCCGTACCTC CCAGCACAAA TGAGTCCTTG 
GATCTGGGCT GCAGCTCCTG TGGGACCCCA CTGCACGACC AGGGGGGTCC TGTGGAGATC 
CTTCCTTTCC TCTACCTCGG CAGTGCCTAC CACGCTGCCC GCAGGGACAT GCTTGATGCC 
CTGGGGATCA CGGCTCTACT GAATGTCTCC TCAGACTGCC CCAATCACTT TGAGGGACAT 
TACCAGTACA AGTGCATCCC GGTAGAAGAT AACCACAAGG CTGACATCAG CTCCTGGTTC 
ATGGAAGCCA TCGAATACAT AGACGCAGTG AAGGACTGCC GAGGGCGAGT GCTGGTTCAC 
TGCCAGGCCG GCATCTCTAG ATCAGCCACC ATCTGCCTGG CCTACCTGAT GATGAAGAAA 
CGGGTGAGGC TGGAGGAGGC TTTCGAGTTC GTCAAGCAGC GCCGTAGCAT CATCTCGCCC 
AACTTCAGCT TCATGGGCCA GTTGCTGCAG TTCGAGTCTC AGGTGCTCAC CACGTCCTGC 
GCAGCGGAGG CCGCCAGCCC TTCCGGGCCC CTGCGGGAGA GGGGGAAGGC CACTCCCACC 
CCCACCTCGC AGTTCGTCTT CAGCTTCCCC GTGTCCGTGG GTGTGCACGC GGCTCCCAGT 
AACCTGCCGT ACCTGCACAG CCCCATCACC ACCTCCCCCA GCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071535.1
CDS	1..1188
Translation	MVTMEELREMDCSVLKRLMNRDENGGTAGSSGGSHGALGLLSGGKCLLLDCRPFLAHSAGYIRGSVNVRCNTIVRRRAKGSVSLEQILPAEEEVRARLRSGLYSAVIVYDERSPRAESLREDSTVSLVVQALRRNAERTDICLLKGGYERFSSEYPEFCSKTKALAAIPPPVPPSTNESLDLGCSSCGTPLHDQGGPVEILPFLYLGSAYHAARRDMLDALGITALLNVSSDCPNHFEGHYQYKCIPVEDNHKADISSWFMEAIEYIDAVKDCRGRVLVHCQAGISRSATICLAYLMMKKRVRLEEAFEFVKQRRSIISPNFSFMGQLLQFESQVLTTSCAAEAASPSGPLRERGKATPTPTSQFVFSFPVSVGVHAAPSNLPYLHSPITTSPSC

Target ORF information:

RefSeq Version	NM_022199.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dual specificity phosphatase 4 (Dusp4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022199.1

ORF Insert Sequence:

ATGGTGACGA TGGAGGAACT GCGGGAGATG GACTGCAGCG TGCTCAAAAG GCTGATGAAC 
CGAGATGAGA ACGGCGGCAC GGCGGGCAGC AGCGGCGGCA GCCACGGCGC CCTGGGGCTG 
CTGAGCGGCG GCAAGTGCTT GCTGCTGGAC TGCAGGCCGT TTCTGGCTCA CAGCGCGGGC 
TACATCCGAG GCTCGGTGAA CGTGCGCTGC AATACCATCG TGCGGCGGAG GGCCAAGGGC 
TCCGTGAGCC TGGAGCAGAT TCTGCCCGCC GAGGAAGAGG TGCGCGCCCG CCTGCGCTCT 
GGCCTCTACT CGGCTGTCAT CGTCTACGAT GAGCGCAGCC CGCGCGCCGA GAGTCTCCGG 
GAGGACAGCA CAGTGTCGCT GGTCGTGCAG GCGTTGCGCC GGAACGCGGA GCGCACAGAC 
ATCTGCCTGC TTAAAGGTGG CTATGAGAGG TTTTCTTCTG AGTACCCAGA ATTCTGCTCT 
AAAACTAAGG CCCTGGCCGC CATCCCACCC CCCGTACCTC CCAGCACAAA TGAGTCCTTG 
GATCTGGGCT GCAGCTCCTG TGGGACCCCA CTGCACGACC AGGGGGGTCC TGTGGAGATC 
CTTCCTTTCC TCTACCTCGG CAGTGCCTAC CACGCTGCCC GCAGGGACAT GCTTGATGCC 
CTGGGGATCA CGGCTCTACT GAATGTCTCC TCAGACTGCC CCAATCACTT TGAGGGACAT 
TACCAGTACA AGTGCATCCC GGTAGAAGAT AACCACAAGG CTGACATCAG CTCCTGGTTC 
ATGGAAGCCA TCGAATACAT AGACGCAGTG AAGGACTGCC GAGGGCGAGT GCTGGTTCAC 
TGCCAGGCCG GCATCTCTAG ATCAGCCACC ATCTGCCTGG CCTACCTGAT GATGAAGAAA 
CGGGTGAGGC TGGAGGAGGC TTTCGAGTTC GTCAAGCAGC GCCGTAGCAT CATCTCGCCC 
AACTTCAGCT TCATGGGCCA GTTGCTGCAG TTCGAGTCTC AGGTGCTCAC CACGTCCTGC 
GCAGCGGAGG CCGCCAGCCC TTCCGGGCCC CTGCGGGAGA GGGGGAAGGC CACTCCCACC 
CCCACCTCGC AGTTCGTCTT CAGCTTCCCC GTGTCCGTGG GTGTGCACGC GGCTCCCAGT 
AACCTGCCGT ACCTGCACAG CCCCATCACC ACCTCCCCCA GCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Redox activation of DUSP4 by N-acetylcysteine protects endothelial cells from Cd??-induced apoptosis.
Free radical biology & medicine74188-199(2014 Sep)
Barajas-Espinosa A,Basye A,Jesse E,Yan H,Quan D,Chen CA

The family-wide structure and function of human dual-specificity protein phosphatases.
Acta crystallographica. Section D, Biological crystallography70(Pt 2)421-35(2014 Feb)
Jeong DG,Wei CH,Ku B,Jeon TJ,Chien PN,Kim JK,Park SY,Hwang HS,Ryu SY,Park H,Kim DS,Kim SJ,Ryu SE

Inhibition of gluconeogenesis through transcriptional activation of EGR1 and DUSP4 by AMP-activated kinase.
The Journal of biological chemistry281(37)27167-77(2006 Sep)
Berasi SP,Huard C,Li D,Shih HH,Sun Y,Zhong W,Paulsen JE,Brown EL,Gimeno RE,Martinez RV

The I(1)-imidazoline receptor in PC12 pheochromocytoma cells reverses NGF-induced ERK activation and induces MKP-2 phosphatase.
Brain research980(1)71-9(2003 Aug)
Edwards L,Ernsberger P

Inhibition of protein tyrosine/mitogen-activated protein kinase phosphatase activity is associated with D2 dopamine receptor supersensitivity in a rat model of Parkinson's disease.
Molecular pharmacology62(6)1356-63(2002 Dec)
Zhen X,Torres C,Cai G,Friedman E

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Dusp4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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