Scfd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Scfd1
Entrez Gene ID	54350
Full Name	sec1 family domain containing 1
Synonyms	RA410,Sly1,rSly1
General protein information	Preferred Names sec1 family domain containing 1 Names sec1 family domain-containing protein 1 SLY1 homolog sly1p syntaxin-binding protein 1-like 2 vesicle transport-related protein Ra410
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q22
Summary	putative syntaxin-binding protein that regulates ER to Golgi transport [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019364.1	NP_062237.1	sec1 family domain-containing protein 1
XM_006240085.3	XP_006240147.1	sec1 family domain-containing protein 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SCFD1	XP_003952508.1
Caenorhabditis elegans (roundworm)	F43D9.3	NP_001122697.2
Homo sapiens (human)	SCFD1	NP_057190.2
	SCFD1	XP_001107671.2
Canis lupus familiaris (dog)	SCFD1	XP_537403.4
Drosophila melanogaster (fruit fly)	Slh	NP_995614.1
Kluyveromyces lactis	KLLA0D18480g	XP_453879.1
Magnaporthe oryzae (rice blast fungus)	MGG_09552	XP_003712189.1
Bos taurus (cattle)	SCFD1	NP_001178294.1
Danio rerio (zebrafish)	sly1	NP_878281.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011358	XP_309291.4
Eremothecium gossypii	AGOS_AGL120W	NP_986547.1
Arabidopsis thaliana (thale cress)	ATSLY1	NP_179389.1
Oryza sativa (rice)	Os03g0620800	NP_001050677.1
Mus musculus (house mouse)	Scfd1	NP_084101.1
Schizosaccharomyces pombe (fission yeast)	sly1	NP_588374.1
Rattus norvegicus (Norway rat)	Scfd1	NP_062237.1
Gallus gallus (chicken)	SCFD1	NP_001186643.1
Saccharomyces cerevisiae (baker's yeast)	SLY1	NP_010475.1
Neurospora crassa	NCU04252	XP_961243.2
Xenopus tropicalis (tropical clawed frog)	scfd1	NP_001135508.1

Gene Ontology
Bibliography

Related articles in PubMed

CCT chaperonin complex is required for efficient delivery of anthrax toxin into the cytosol of host cells.
Slater LH, Hett EC, Clatworthy AE, Mark KG, Hung DT
Proceedings of the National Academy of Sciences of the United States of America110(24)9932-7(2013 Jun)

Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex.
Renna M, Schaffner C, Winslow AR, Menzies FM, Peden AA, Floto RA, Rubinsztein DC
Journal of cell science124(Pt 3)469-82(2011 Feb)

Direct interaction between the COG complex and the SM protein, Sly1, is required for Golgi SNARE pairing.
Laufman O, Kedan A, Hong W, Lev S
The EMBO journal28(14)2006-17(2009 Jul)

RA410/Sly1 suppresses MPP+ and 6-hydroxydopamine-induced cell death in SH-SY5Y cells.
Bando Y, Katayama T, Taniguchi M, Ishibashi T, Matsuo N, Ogawa S, Tohyama M
Neurobiology of disease18(1)143-51(2005 Feb)

Three-dimensional structure of the rSly1 N-terminal domain reveals a conformational change induced by binding to syntaxin 5.
Ara? D, Dulubova I, Pei J, Huryeva I, Grishin NV, Rizo J
Journal of molecular biology346(2)589-601(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that RA410/Sly1 plays an important cytoprotective role in MPP+ and 6-OHDA-induced cellular perturbation.
Title: RA410/Sly1 suppresses MPP+ and 6-hydroxydopamine-induced cell death in SH-SY5Y cells.

elucidated the structure in solution of the isolated N-terminal domain of rat Sly1 and analyzed its complex with an N-terminal peptide of rat syntaxin 5 by NMR spectroscopy
Title: Three-dimensional structure of the rSly1 N-terminal domain reveals a conformational change induced by binding to syntaxin 5.

results argue that although rsly1 does not seem to regulate the availability of syntaxin 5, its function is intimately associated with syntaxin binding, perhaps promoting a later step in SNARE complex formation or function
Title: rsly1 binding to syntaxin 5 is required for endoplasmic reticulum-to-Golgi transport but does not promote SNARE motif accessibility.

The following Scfd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Scfd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04562	NM_019364.1 Latest version!	Rattus norvegicus sec1 family domain containing 1 (Scfd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa29335	XM_006240085.3 Latest version!	Rattus norvegicus sec1 family domain containing 1 (Scfd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Scfd1 ( NM_019364.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062237.1 Rattus norvegicus sec1 family domain containing 1 (Scfd1), mRNA.

Price & Availability↑

CloneID	ORa04562
Clone ID Related Accession (Same CDS sequence)	NM_019364.1
Accession Version	NM_019364.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	sec1 family domain-containing protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D79221.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D79221.1, U57687.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTGGGAA GCAAGATGGC GGCCAGCATT CGGGAAAGGC AAACAGTGGC TCTGAAGCGG 
ATGTTGAATT TCAATGTGCC TCATGTTAAA AACAGTCCTG GAGAACCCGT ATGGAAGGTA 
CTCATCTATG ACAGATTTGG CCAAGATATC ATCTCTCCTC TGCTGTCTGT GAAGGAGCTG 
AGAGACATGG GCATCACCCT GCATCTCCTT TTGCACTCAG ACCGAGATCC AATTCGAGAT 
GTTCCTGCGG TGTACTTTGT GATGCCAACC GAAGAAAATA TTGACAGACT GTGCCAGGAT 
CTTCGAAATC AGCTCTATGA ATCCTATTAT TTAAATTTTA TTTCTGCGAT TTCAAGAAGT 
AAACTGGAAG ACATTGCAAA TGCAGCATTG GCCGCTAATG CAGTCACACA GGTTGCCAAG 
GTTTTTGACC AGTATCTCAA TTTTATTACT TTGGAAGAGG ACATGTTTGT ATTATGTAAT 
CAAAATAAGG AACTTGTTTC ATATCGGGCC ATTAATAGGC CAGATATCAC AGACACAGAG 
ATGGAGACTG TTATGGACAC TATTGTTGAC AGCCTCTTCT GCTTTTTTGT TACATTAGGT 
GCTGTTCCCA TCATCCGATG CTCAAGAGGA ACGGCAGCAG AAATGGTGGC AGTGAAACTA 
GATAAAAAAC TGCGGGAGAA TCTAAGAGAT GCAAGAAACA GCCTTTTTAC TGGTGATCCA 
CTTGGGACTG GCCAGTTCAG CTTCCAAAGG CCCTTATTAG TCCTTGTGGA CAGAAACATT 
GACTTGGCAA CGCCTCTGCA CCATACGTGG ACATACCAAG CGCTGGTACA CGATGTCCTG 
GATTTCCACT TAAACAGAGT AAATTTGGAA GAATCTACAG GAGTGGAAAA TTCTCCAACT 
GGTGCTAGAC CAAAGAGGAA AAACAAGAAG TCTTACGATT TAACTCCAGT TGATAAATTT 
TGGCAGAAAC ATAAAGGAAG TCCATTCCCA GAAGTCGCAG AATCAGTCCA ACAAGAACTA 
GAATCTTACA GAGCACAAGA AGATGAGGTC AAACGACTGA AGAGCATTAT GGGCCTAGAA 
GGAGAGGACG AAGGAGCCAT CAGCATGCTT TCTGATAACA CTGCTAAGCT CACATCAGCT 
GTCAGTTCTT TGCCAGAACT CCTTGAAAAA AAAAGACTTA TCGATCTCCA TACAAATGTC 
GCCACTGCTG TTTTAGAACA CATAAAGGCA AGAAAACTGG ATGTATATTT TGAATATGAA 
GAAAAAATAA TGAGCAAGAC TACTCTGGAT AAGTCCCTTC TCGACGTCAT ATCTGACCCT 
GACGCAGGGA CTCCGGAAGA CAAAATGAGG CTGTTTCTTA TCTACTACAT AAGCGCTCAG 
CAGGCACCAT CTGAGGTTGA TTTGGAGCAG TATAAAAAGG CTTTAACAGA TGCAGGATGC 
AACCTTAGCC CTTTACAGTA TATCAAACAG TGGAAGGCTT TTGCCAAGAT GGCCTCAACT 
CCTGCCAGCT ACGGAAACAC TACCACTAAA CCAATGGGTC TCTTGTCCCG AGTCATGAAT 
ACAGGATCCC AGTTTGTGAT GGAAGGCGTC AAGAACCTGG TATTGAAGCA GCAGAATCTA 
CCTGTTACTC GGATTTTAGA CAATCTCATG GAGATGAAGT CAAACCCCGA GACTGATGAT 
TACAGATATT TTGATCCCAA AATGCTGCGG AGCAATGACA GCTCAGTTCC TAGGAACAAA 
AGTCCATTCC AAGAGGCCAT TGTCTTTGTG GTAGGAGGAG GCAACTATAT TGAGTATCAG 
AATCTTGTTG ACTACATAAA GGGAAAGCAA GGCAAGCATA TTTTGTATGG CTGCAGTGAG 
ATTTTTAATG CTACACAGTT CATAAAACAG CTGTCACAGC TTGGACAAAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062237.1
CDS	14..1927
Translation	MVGSKMAASIRERQTVALKRMLNFNVPHVKNSPGEPVWKVLIYDRFGQDIISPLLSVKELRDMGITLHLLLHSDRDPIRDVPAVYFVMPTEENIDRLCQDLRNQLYESYYLNFISAISRSKLEDIANAALAANAVTQVAKVFDQYLNFITLEEDMFVLCNQNKELVSYRAINRPDITDTEMETVMDTIVDSLFCFFVTLGAVPIIRCSRGTAAEMVAVKLDKKLRENLRDARNSLFTGDPLGTGQFSFQRPLLVLVDRNIDLATPLHHTWTYQALVHDVLDFHLNRVNLEESTGVENSPTGARPKRKNKKSYDLTPVDKFWQKHKGSPFPEVAESVQQELESYRAQEDEVKRLKSIMGLEGEDEGAISMLSDNTAKLTSAVSSLPELLEKKRLIDLHTNVATAVLEHIKARKLDVYFEYEEKIMSKTTLDKSLLDVISDPDAGTPEDKMRLFLIYYISAQQAPSEVDLEQYKKALTDAGCNLSPLQYIKQWKAFAKMASTPASYGNTTTKPMGLLSRVMNTGSQFVMEGVKNLVLKQQNLPVTRILDNLMEMKSNPETDDYRYFDPKMLRSNDSSVPRNKSPFQEAIVFVVGGGNYIEYQNLVDYIKGKQGKHILYGCSEIFNATQFIKQLSQLGQK

Target ORF information:

RefSeq Version	NM_019364.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sec1 family domain containing 1 (Scfd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019364.1

ORF Insert Sequence:

ATGGTGGGAA GCAAGATGGC GGCCAGCATT CGGGAAAGGC AAACAGTGGC TCTGAAGCGG 
ATGTTGAATT TCAATGTGCC TCATGTTAAA AACAGTCCTG GAGAACCCGT ATGGAAGGTA 
CTCATCTATG ACAGATTTGG CCAAGATATC ATCTCTCCTC TGCTGTCTGT GAAGGAGCTG 
AGAGACATGG GCATCACCCT GCATCTCCTT TTGCACTCAG ACCGAGATCC AATTCGAGAT 
GTTCCTGCGG TGTACTTTGT GATGCCAACC GAAGAAAATA TTGACAGACT GTGCCAGGAT 
CTTCGAAATC AGCTCTATGA ATCCTATTAT TTAAATTTTA TTTCTGCGAT TTCAAGAAGT 
AAACTGGAAG ACATTGCAAA TGCAGCATTG GCCGCTAATG CAGTCACACA GGTTGCCAAG 
GTTTTTGACC AGTATCTCAA TTTTATTACT TTGGAAGAGG ACATGTTTGT ATTATGTAAT 
CAAAATAAGG AACTTGTTTC ATATCGGGCC ATTAATAGGC CAGATATCAC AGACACAGAG 
ATGGAGACTG TTATGGACAC TATTGTTGAC AGCCTCTTCT GCTTTTTTGT TACATTAGGT 
GCTGTTCCCA TCATCCGATG CTCAAGAGGA ACGGCAGCAG AAATGGTGGC AGTGAAACTA 
GATAAAAAAC TGCGGGAGAA TCTAAGAGAT GCAAGAAACA GCCTTTTTAC TGGTGATCCA 
CTTGGGACTG GCCAGTTCAG CTTCCAAAGG CCCTTATTAG TCCTTGTGGA CAGAAACATT 
GACTTGGCAA CGCCTCTGCA CCATACGTGG ACATACCAAG CGCTGGTACA CGATGTCCTG 
GATTTCCACT TAAACAGAGT AAATTTGGAA GAATCTACAG GAGTGGAAAA TTCTCCAACT 
GGTGCTAGAC CAAAGAGGAA AAACAAGAAG TCTTACGATT TAACTCCAGT TGATAAATTT 
TGGCAGAAAC ATAAAGGAAG TCCATTCCCA GAAGTCGCAG AATCAGTCCA ACAAGAACTA 
GAATCTTACA GAGCACAAGA AGATGAGGTC AAACGACTGA AGAGCATTAT GGGCCTAGAA 
GGAGAGGACG AAGGAGCCAT CAGCATGCTT TCTGATAACA CTGCTAAGCT CACATCAGCT 
GTCAGTTCTT TGCCAGAACT CCTTGAAAAA AAAAGACTTA TCGATCTCCA TACAAATGTC 
GCCACTGCTG TTTTAGAACA CATAAAGGCA AGAAAACTGG ATGTATATTT TGAATATGAA 
GAAAAAATAA TGAGCAAGAC TACTCTGGAT AAGTCCCTTC TCGACGTCAT ATCTGACCCT 
GACGCAGGGA CTCCGGAAGA CAAAATGAGG CTGTTTCTTA TCTACTACAT AAGCGCTCAG 
CAGGCACCAT CTGAGGTTGA TTTGGAGCAG TATAAAAAGG CTTTAACAGA TGCAGGATGC 
AACCTTAGCC CTTTACAGTA TATCAAACAG TGGAAGGCTT TTGCCAAGAT GGCCTCAACT 
CCTGCCAGCT ACGGAAACAC TACCACTAAA CCAATGGGTC TCTTGTCCCG AGTCATGAAT 
ACAGGATCCC AGTTTGTGAT GGAAGGCGTC AAGAACCTGG TATTGAAGCA GCAGAATCTA 
CCTGTTACTC GGATTTTAGA CAATCTCATG GAGATGAAGT CAAACCCCGA GACTGATGAT 
TACAGATATT TTGATCCCAA AATGCTGCGG AGCAATGACA GCTCAGTTCC TAGGAACAAA 
AGTCCATTCC AAGAGGCCAT TGTCTTTGTG GTAGGAGGAG GCAACTATAT TGAGTATCAG 
AATCTTGTTG ACTACATAAA GGGAAAGCAA GGCAAGCATA TTTTGTATGG CTGCAGTGAG 
ATTTTTAATG CTACACAGTT CATAAAACAG CTGTCACAGC TTGGACAAAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scfd1 ( XM_006240085.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240147.1 Rattus norvegicus sec1 family domain containing 1 (Scfd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa29335
Clone ID Related Accession (Same CDS sequence)	XM_006240085.3
Accession Version	XM_006240085.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sec1 family domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240085.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGAA GCAAGATGGC GGCCAGCATT CGGGAAAGGC AAACAGTGGC TCTGAAGCGG 
ATGTTGAATT TCAATGTGCC TCATGTTAAA AACAGTCCTG GAGAACCCGT ATGGAAGGTA 
CTCATCTATG ACAGATTTGG CCAAGATATC ATCTCTCCTC TGCTGTCTGT GAAGGAGCTG 
AGAGACATGG GCATCACCCT GCATCTCCTT TTGCACTCAG ACCGAGATCC AATTCGAGAT 
GTTCCTGCGG TGTACTTTGT GATGCCAACC GAAGAAAATA TTGACAGACT GTGCCAGGAT 
CTTCGAAATC AGCTCTATGA ATCCTATTAT TTAAATTTTA TTTCTGCGAT TTCAAGAAGT 
AAACTGGAAG ACATTGCAAA TGCAGCATTG GCCGCTAATG CAGTCACACA GGTTGCCAAG 
GTTTTTGACC AGTATCTCAA TTTTATTACT TTGGAAGAGG ACATGTTTGT ATTATGTAAT 
CAAAATAAGG AACTTGTTTC ATATCGGGCC ATTAATAGGC CAGATATCAC AGACACAGAG 
ATGGAGACTG TTATGGACAC TATTGTTGAC AGCCTCTTCT GCTTTTTTGT TACATTAGGT 
GCTGTTCCCA TCATCCGATG CTCAAGAGGA ACGGCAGCAG AAATGGTGGC AGTGAAACTA 
GATAAAAAAC TGCGGGAGAA TCTAAGAGAT GCAAGAAACA GCCTTTTTAC TGGTGATCCA 
CTTGGGACTG GCCAGTTCAG CTTCCAAAGG CCCTTATTAG TCCTTGTGGA CAGAAACATT 
GACTTGGCAA CGCCTCTGCA CCATACGTGG ACATACCAAG CGCTGGTACA CGATGTCCTG 
GATTTCCACT TAAACAGAGT AAATTTGGAA GAATCTACAG GAGTGGAAAA TTCTCCAACT 
GGTGCTAGAC CAAAGAGGAA AAACAAGAAG TCTTACGATT TAACTCCAGT TGATAAATTT 
TGGCAGAAAC ATAAAGGAAG TCCATTCCCA GAAGTCGCAG AATCAGTCCA ACAAGAACTA 
GAATCTTACA GAGCACAAGA AGATGAGGTC AAACGACTGA AGAGCATTAT GGGCCTAGAA 
GGAGAGGACG AAGGAGCCAT CAGCATGCTT TCTGATAACA CTGCTAAGCT CACATCAGCT 
GTCAGTTCTT TGCCAGAACT CCTTGAAAAA AAAAGACTTA TCGATCTCCA TACAAATGTC 
GCCACTGCTG TTTTAGAACA CATAAAGGCA AGAAAACTGG ATGTATATTT TGAATATGAA 
GAAAAAATAA TGAGCAAGAC TACTCTGGAT AAGTCCCTTC TCGACGTCAT ATCTGACCCT 
GACGCAGGGA CTCCGGAAGA CAAAATGAGG CTGTTTCTTA TCTACTACAT AAGCGCTCAG 
CAGGCACCAT CTGAGGTTGA TTTGGAGCAG TATAAAAAGG CTTTAACAGA TGCAGGATGC 
AACCTTAGCC CTTTACAGTA TATCAAACAG TGGAAGGCTT TTGCCAAGAT GGCCTCAACT 
CCTGCCAGCT ACGGAAACAC TACCACTAAA CCAATGGGTC TCTTGTCCCG AGTCATGAAT 
ACAGGATCCC AGTTTGTGAT GGAAGGCGTC AAGAACCTGG TATTGAAGCA GCAGAATCTA 
CCTGTTACTC GGATTTTAGA CAATCTCATG GAGATGAAGT CAAACCCCGA GACTGATGAT 
TACAGATATT TTGATCCCAA AATGCTGCGG AGCAATGACA GCTCCTTCCT CTACAAAACT 
GAAGACCCCC CTGCTTCCCA AGAAGGTGGA CCTGGAATCC CTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240147.1
CDS	64..1851
Translation	MVGSKMAASIRERQTVALKRMLNFNVPHVKNSPGEPVWKVLIYDRFGQDIISPLLSVKELRDMGITLHLLLHSDRDPIRDVPAVYFVMPTEENIDRLCQDLRNQLYESYYLNFISAISRSKLEDIANAALAANAVTQVAKVFDQYLNFITLEEDMFVLCNQNKELVSYRAINRPDITDTEMETVMDTIVDSLFCFFVTLGAVPIIRCSRGTAAEMVAVKLDKKLRENLRDARNSLFTGDPLGTGQFSFQRPLLVLVDRNIDLATPLHHTWTYQALVHDVLDFHLNRVNLEESTGVENSPTGARPKRKNKKSYDLTPVDKFWQKHKGSPFPEVAESVQQELESYRAQEDEVKRLKSIMGLEGEDEGAISMLSDNTAKLTSAVSSLPELLEKKRLIDLHTNVATAVLEHIKARKLDVYFEYEEKIMSKTTLDKSLLDVISDPDAGTPEDKMRLFLIYYISAQQAPSEVDLEQYKKALTDAGCNLSPLQYIKQWKAFAKMASTPASYGNTTTKPMGLLSRVMNTGSQFVMEGVKNLVLKQQNLPVTRILDNLMEMKSNPETDDYRYFDPKMLRSNDSSFLYKTEDPPASQEGGPGIPS

Target ORF information:

RefSeq Version	XM_006240085.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sec1 family domain containing 1 (Scfd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240085.3

ORF Insert Sequence:

ATGGTGGGAA GCAAGATGGC GGCCAGCATT CGGGAAAGGC AAACAGTGGC TCTGAAGCGG 
ATGTTGAATT TCAATGTGCC TCATGTTAAA AACAGTCCTG GAGAACCCGT ATGGAAGGTA 
CTCATCTATG ACAGATTTGG CCAAGATATC ATCTCTCCTC TGCTGTCTGT GAAGGAGCTG 
AGAGACATGG GCATCACCCT GCATCTCCTT TTGCACTCAG ACCGAGATCC AATTCGAGAT 
GTTCCTGCGG TGTACTTTGT GATGCCAACC GAAGAAAATA TTGACAGACT GTGCCAGGAT 
CTTCGAAATC AGCTCTATGA ATCCTATTAT TTAAATTTTA TTTCTGCGAT TTCAAGAAGT 
AAACTGGAAG ACATTGCAAA TGCAGCATTG GCCGCTAATG CAGTCACACA GGTTGCCAAG 
GTTTTTGACC AGTATCTCAA TTTTATTACT TTGGAAGAGG ACATGTTTGT ATTATGTAAT 
CAAAATAAGG AACTTGTTTC ATATCGGGCC ATTAATAGGC CAGATATCAC AGACACAGAG 
ATGGAGACTG TTATGGACAC TATTGTTGAC AGCCTCTTCT GCTTTTTTGT TACATTAGGT 
GCTGTTCCCA TCATCCGATG CTCAAGAGGA ACGGCAGCAG AAATGGTGGC AGTGAAACTA 
GATAAAAAAC TGCGGGAGAA TCTAAGAGAT GCAAGAAACA GCCTTTTTAC TGGTGATCCA 
CTTGGGACTG GCCAGTTCAG CTTCCAAAGG CCCTTATTAG TCCTTGTGGA CAGAAACATT 
GACTTGGCAA CGCCTCTGCA CCATACGTGG ACATACCAAG CGCTGGTACA CGATGTCCTG 
GATTTCCACT TAAACAGAGT AAATTTGGAA GAATCTACAG GAGTGGAAAA TTCTCCAACT 
GGTGCTAGAC CAAAGAGGAA AAACAAGAAG TCTTACGATT TAACTCCAGT TGATAAATTT 
TGGCAGAAAC ATAAAGGAAG TCCATTCCCA GAAGTCGCAG AATCAGTCCA ACAAGAACTA 
GAATCTTACA GAGCACAAGA AGATGAGGTC AAACGACTGA AGAGCATTAT GGGCCTAGAA 
GGAGAGGACG AAGGAGCCAT CAGCATGCTT TCTGATAACA CTGCTAAGCT CACATCAGCT 
GTCAGTTCTT TGCCAGAACT CCTTGAAAAA AAAAGACTTA TCGATCTCCA TACAAATGTC 
GCCACTGCTG TTTTAGAACA CATAAAGGCA AGAAAACTGG ATGTATATTT TGAATATGAA 
GAAAAAATAA TGAGCAAGAC TACTCTGGAT AAGTCCCTTC TCGACGTCAT ATCTGACCCT 
GACGCAGGGA CTCCGGAAGA CAAAATGAGG CTGTTTCTTA TCTACTACAT AAGCGCTCAG 
CAGGCACCAT CTGAGGTTGA TTTGGAGCAG TATAAAAAGG CTTTAACAGA TGCAGGATGC 
AACCTTAGCC CTTTACAGTA TATCAAACAG TGGAAGGCTT TTGCCAAGAT GGCCTCAACT 
CCTGCCAGCT ACGGAAACAC TACCACTAAA CCAATGGGTC TCTTGTCCCG AGTCATGAAT 
ACAGGATCCC AGTTTGTGAT GGAAGGCGTC AAGAACCTGG TATTGAAGCA GCAGAATCTA 
CCTGTTACTC GGATTTTAGA CAATCTCATG GAGATGAAGT CAAACCCCGA GACTGATGAT 
TACAGATATT TTGATCCCAA AATGCTGCGG AGCAATGACA GCTCCTTCCT CTACAAAACT 
GAAGACCCCC CTGCTTCCCA AGAAGGTGGA CCTGGAATCC CTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CCT chaperonin complex is required for efficient delivery of anthrax toxin into the cytosol of host cells.
Proceedings of the National Academy of Sciences of the United States of America110(24)9932-7(2013 Jun)
Slater LH,Hett EC,Clatworthy AE,Mark KG,Hung DT

Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex.
Journal of cell science124(Pt 3)469-82(2011 Feb)
Renna M,Schaffner C,Winslow AR,Menzies FM,Peden AA,Floto RA,Rubinsztein DC

Direct interaction between the COG complex and the SM protein, Sly1, is required for Golgi SNARE pairing.
The EMBO journal28(14)2006-17(2009 Jul)
Laufman O,Kedan A,Hong W,Lev S

RA410/Sly1 suppresses MPP+ and 6-hydroxydopamine-induced cell death in SH-SY5Y cells.
Neurobiology of disease18(1)143-51(2005 Feb)
Bando Y,Katayama T,Taniguchi M,Ishibashi T,Matsuo N,Ogawa S,Tohyama M

Three-dimensional structure of the rSly1 N-terminal domain reveals a conformational change induced by binding to syntaxin 5.
Journal of molecular biology346(2)589-601(2005 Feb)
Ara? D,Dulubova I,Pei J,Huryeva I,Grishin NV,Rizo J

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Scfd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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