H1f7 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	H1f7
Entrez Gene ID	500928
Full Name	H1 histone family, member N, testis-specific
General protein information	Preferred Names H1 histone family, member N, testis-specific Names testis-specific H1 histone haploid germ cell-specific nuclear protein 1 histone H1t2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q36

mRNA and Protein(s)

mRNA	Protein	Name
NM_001024356.1	NP_001019527.1	testis-specific H1 histone

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Contribution of the two genes encoding histone variant h3.3 to viability and fertility in mice.
Tang MC, Jacobs SA, Mattiske DM, Soh YM, Graham AN, Tran A, Lim SL, Hudson DF, Kalitsis P, O'Bryan MK, Wong LH, Mann JR
PLoS genetics11(2)e1004964(2015 Feb)

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

Pericentric heterochromatin reprogramming by new histone variants during mouse spermiogenesis.
Govin J, Escoffier E, Rousseaux S, Kuhn L, Ferro M, Th?venon J, Catena R, Davidson I, Garin J, Khochbin S, Caron C
The Journal of cell biology176(3)283-94(2007 Jan)

Changes in intranuclear chromatin architecture induce bipolar nuclear localization of histone variant H1T2 in male haploid spermatids.
Catena R, Ronfani L, Sassone-Corsi P, Davidson I
Developmental biology296(1)231-238(2006 Aug)

Polar nuclear localization of H1T2, a histone H1 variant, required for spermatid elongation and DNA condensation during spermiogenesis.
Martianov I, Brancorsini S, Catena R, Gansmuller A, Kotaja N, Parvinen M, Sassone-Corsi P, Davidson I
Proceedings of the National Academy of Sciences of the United States of America102(8)2808-13(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following H1f7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H1f7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42601	NM_001024356.1 Latest version!	Rattus norvegicus H1.7 linker histone (H1f7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H1f7 ( NM_001024356.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001019527.1 Rattus norvegicus H1.7 linker histone (H1f7), mRNA.

Price & Availability↑

CloneID	ORa42601
Clone ID Related Accession (Same CDS sequence)	NM_001024356.1
Accession Version	NM_001024356.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Rattus norvegicus(Norway rat)
Product	testis-specific H1 histone
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085950.1. On May 27, 2005 this sequence version replaced XM_576337.1. ##Evidence-Data-START## Transcript exon combination :: BC085950.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCTGAGG CCGCCCAGCC CACTGGGGAA TCCCAAGGCG CAGAACTTAC TATCCAGATC 
CAGCAGCCTG TGGAGAGAGC CCTGAGGACG CCGGTCAGGC GGGGTGCACA GTCCGTGCTC 
AGAGTGTCCC AGCTGCTGCT CAGGGCCATT GCTGGCCACC AGCGCCTGAC TCTGGCTGCA 
CTCAAGAAGG AGCTGGGAAA CGCCGGCTAC GAAGTGCGCA GGAAGATCTC CTCTCACCAA 
GCGGGGGACT CCACCAGGTC GGAGAAATAC ACGCTCCTGA GGGTCAGTGG CAGCGACGCT 
GCAGGCTACT TCAGGGTCTG GAAAATTTCA AAGCCTAGGA GAAAGGCGCC ACGGTCCCGG 
TTGACACTGG GCAGCCACTC TCACGGGAAG ACTGTGCTCA AATCCCCAAG ACCACTCAGG 
CCCCGCTCGC GTCGCAAGGC GGCCAAGAAG GCCAGAGAAG TCTGGAGACG CAAGGCCAGG 
GCTTTGAAGG CAAGGTCCAG GAGGGCCAGA TCACTGGCCA GATCAATGGT CAGATCAAGG 
GCCAGCTCAA GGGCCAGCTC AAGGGCCAGG TCAAGGGCCA GGTCAAGAGC CCGGTCAAGG 
GCCAGGTCAA GGGCTAGGTC AAGAGCCAGC TCAAGGGCCC GGTCAAGCGC CCGGTCAAGC 
GCCCGGTCAA GTGCCCGGTC AAGCGTGCGG TCAAGCGTGC GGTCAAGCGC CCGGTCAAGC 
GCCCGGTCAA GCATCAGGTC AAGAGCCAGG TCAAGGGCAA GGACAAGGGC CAGGTCTAGA 
GCTAAGGACC TAGTGCGTTC CAAGGCCAGG GAACAGGCTC GTACCAGAGC CAGGGAACAA 
GCTCATGCCA GAGCCAGGAC ACACGACTGT GTCAGGGCCA AGGCACAGGA GTATGTCAGG 
GCCAAAGAGC AGCAGTACGT CAGTGCCAAG GAGCAGGAGT ATGTCAGGAC CAAGGAGCAG 
GAGTGTGCCA AGGCCAGGGA ACAGATGCGT ATCGGAGCCA TGGAGGAAGC CAGGATCAAG 
GCGATCGACA ACAGAGTACA GACTACCATG GAAGATACCA GTCCTTGGTC TACAGATGAG 
ATGAGGTCAA GAACCAAACC TAGGGAAGAG AAGAGACAGG AACCCGAGAG GCCAGTAAAA 
CAAACCAACC AGAAGCCAGC TGTGGTCAAA GTAGACAACG CTCCCAGCAG ACAAGGAAAA 
ACATGCACAA AGTCTTCTAC TAAAAGTGGT CATCCTGGGT GCTCAGGGAC TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019527.1
CDS	26..1282
Translation	MAEAAQPTGESQGAELTIQIQQPVERALRTPVRRGAQSVLRVSQLLLRAIAGHQRLTLAALKKELGNAGYEVRRKISSHQAGDSTRSEKYTLLRVSGSDAAGYFRVWKISKPRRKAPRSRLTLGSHSHGKTVLKSPRPLRPRSRRKAAKKAREVWRRKARALKARSRRARSLARSMVRSRASSRASSRARSRARSRARSRARSRARSRASSRARSSARSSARSSARSSVRSSVRSSARSSARSSIRSRARSRARTRARSRAKDLVRSKAREQARTRAREQAHARARTHDCVRAKAQEYVRAKEQQYVSAKEQEYVRTKEQECAKAREQMRIGAMEEARIKAIDNRVQTTMEDTSPWSTDEMRSRTKPREEKRQEPERPVKQTNQKPAVVKVDNAPSRQGKTCTKSSTKSGHPGCSGTS

Target ORF information:

RefSeq Version	NM_001024356.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus H1.7 linker histone (H1f7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024356.1

ORF Insert Sequence:

ATGGCTGAGG CCGCCCAGCC CACTGGGGAA TCCCAAGGCG CAGAACTTAC TATCCAGATC 
CAGCAGCCTG TGGAGAGAGC CCTGAGGACG CCGGTCAGGC GGGGTGCACA GTCCGTGCTC 
AGAGTGTCCC AGCTGCTGCT CAGGGCCATT GCTGGCCACC AGCGCCTGAC TCTGGCTGCA 
CTCAAGAAGG AGCTGGGAAA CGCCGGCTAC GAAGTGCGCA GGAAGATCTC CTCTCACCAA 
GCGGGGGACT CCACCAGGTC GGAGAAATAC ACGCTCCTGA GGGTCAGTGG CAGCGACGCT 
GCAGGCTACT TCAGGGTCTG GAAAATTTCA AAGCCTAGGA GAAAGGCGCC ACGGTCCCGG 
TTGACACTGG GCAGCCACTC TCACGGGAAG ACTGTGCTCA AATCCCCAAG ACCACTCAGG 
CCCCGCTCGC GTCGCAAGGC GGCCAAGAAG GCCAGAGAAG TCTGGAGACG CAAGGCCAGG 
GCTTTGAAGG CAAGGTCCAG GAGGGCCAGA TCACTGGCCA GATCAATGGT CAGATCAAGG 
GCCAGCTCAA GGGCCAGCTC AAGGGCCAGG TCAAGGGCCA GGTCAAGAGC CCGGTCAAGG 
GCCAGGTCAA GGGCTAGGTC AAGAGCCAGC TCAAGGGCCC GGTCAAGCGC CCGGTCAAGC 
GCCCGGTCAA GTGCCCGGTC AAGCGTGCGG TCAAGCGTGC GGTCAAGCGC CCGGTCAAGC 
GCCCGGTCAA GCATCAGGTC AAGAGCCAGG TCAAGGGCAA GGACAAGGGC CAGGTCTAGA 
GCTAAGGACC TAGTGCGTTC CAAGGCCAGG GAACAGGCTC GTACCAGAGC CAGGGAACAA 
GCTCATGCCA GAGCCAGGAC ACACGACTGT GTCAGGGCCA AGGCACAGGA GTATGTCAGG 
GCCAAAGAGC AGCAGTACGT CAGTGCCAAG GAGCAGGAGT ATGTCAGGAC CAAGGAGCAG 
GAGTGTGCCA AGGCCAGGGA ACAGATGCGT ATCGGAGCCA TGGAGGAAGC CAGGATCAAG 
GCGATCGACA ACAGAGTACA GACTACCATG GAAGATACCA GTCCTTGGTC TACAGATGAG 
ATGAGGTCAA GAACCAAACC TAGGGAAGAG AAGAGACAGG AACCCGAGAG GCCAGTAAAA 
CAAACCAACC AGAAGCCAGC TGTGGTCAAA GTAGACAACG CTCCCAGCAG ACAAGGAAAA 
ACATGCACAA AGTCTTCTAC TAAAAGTGGT CATCCTGGGT GCTCAGGGAC TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Contribution of the two genes encoding histone variant h3.3 to viability and fertility in mice.
PLoS genetics11(2)e1004964(2015 Feb)
Tang MC,Jacobs SA,Mattiske DM,Soh YM,Graham AN,Tran A,Lim SL,Hudson DF,Kalitsis P,O'Bryan MK,Wong LH,Mann JR

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballesc? JL,Oliva R

Pericentric heterochromatin reprogramming by new histone variants during mouse spermiogenesis.
The Journal of cell biology176(3)283-94(2007 Jan)
Govin J,Escoffier E,Rousseaux S,Kuhn L,Ferro M,Th?venon J,Catena R,Davidson I,Garin J,Khochbin S,Caron C

Changes in intranuclear chromatin architecture induce bipolar nuclear localization of histone variant H1T2 in male haploid spermatids.
Developmental biology296(1)231-238(2006 Aug)
Catena R,Ronfani L,Sassone-Corsi P,Davidson I

Polar nuclear localization of H1T2, a histone H1 variant, required for spermatid elongation and DNA condensation during spermiogenesis.
Proceedings of the National Academy of Sciences of the United States of America102(8)2808-13(2005 Feb)
Martianov I,Brancorsini S,Catena R,Gansmuller A,Kotaja N,Parvinen M,Sassone-Corsi P,Davidson I