Purb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Purb
Entrez Gene ID	498407
Full Name	purine rich element binding protein B
Synonyms	pur-beta
General protein information	Preferred Names purine rich element binding protein B Names transcriptional activator protein Pur-beta transcription factor Pur-beta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q21

mRNA and Protein(s)

mRNA	Protein	Name
NM_001017503.1	NP_001017503.1	transcriptional activator protein Pur-beta

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	H2AFV	XP_005642237.1
Danio rerio (zebrafish)	purbb	NP_956841.1
Pan troglodytes (chimpanzee)	PURB	XP_003951050.1
Xenopus tropicalis (tropical clawed frog)	LOC100495008	XP_004912630.1
Homo sapiens (human)	PURB	NP_150093.1
Bos taurus (cattle)	PURB	NP_001193561.1
Mus musculus (house mouse)	Purb	NP_035351.1
Rattus norvegicus (Norway rat)	Purb	NP_001017503.1
Danio rerio (zebrafish)	purba	NP_001153631.1

Gene Ontology
Bibliography

Related articles in PubMed

linc-HOXA1 is a noncoding RNA that represses Hoxa1 transcription in cis.
Maamar H, Cabili MN, Rinn J, Raj A
Genes & development27(11)1260-71(2013 Jun)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

The galectin-3 gene promoter binding proteins in the liver of rats 48-h post-treatment with CCl4.
Li F, Kato I, Kawaguchi H, Takasawa K, Hibino Y, Hiraga K
Gene36746-55(2006 Feb)

A binding site for Pur alpha and Pur beta is structurally unstable and is required for replication in vivo from the rat aldolase B origin.
Shimotai Y, Minami H, Saitoh Y, Onodera Y, Mishima Y, Kelm RJ Jr, Tsutsumi K
Biochemical and biophysical research communications340(2)517-25(2006 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Purb may have role in DNA replication initiation. [purine rich element binding protein B; Purb]
Title: A binding site for Pur alpha and Pur beta is structurally unstable and is required for replication in vivo from the rat aldolase B origin.

The following Purb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Purb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42420	NM_001017503.1 Latest version!	Rattus norvegicus purine rich element binding protein B (Purb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Purb ( NM_001017503.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001017503.1 Rattus norvegicus purine rich element binding protein B (Purb), mRNA.

Price & Availability↑

CloneID	ORa42420
Clone ID Related Accession (Same CDS sequence)	NM_001017503.1
Accession Version	NM_001017503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	transcriptional activator protein Pur-beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB182574.1. On Apr 29, 2005 this sequence version replaced XM_573651.1. ##Evidence-Data-START## Transcript exon combination :: AB182574.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCGGACG GCGACAGCGG CAGCGAGCGC GGTGGCGGCG GGCCCGGCAG CTTCCAGCCC 
GCGCCCCGCG GCGGCGGAGG GCCTGGCGGC GAGCAGGAGA CGCAGGAGCT GGCCTCGAAG 
CGGCTGGACA TCCAGAACAA GCGCTTCTAC CTGGACGTGA AGCAGAACGC CAAGGGCCGC 
TTCCTCAAGA TCGCCGAGGT GGGCGCGGGC GGCTCCAAGA GCCGCCTCAC GCTCTCGATG 
GCGGTGGCCG CCGAGTTCCG CGACTCGCTG GGCGACTTCA TCGAGCACTA CGCGCAGCTG 
GGTCCCAGCA GCCCGGAGCA GCTGGCGGCG GGCGCCGAGG AGGGCGGCGG GCCGCGCCGC 
GCGCTTAAGA GCGAATTCCT GGTGCGCGAG AACCGCAAGT ACTACCTGGA CCTCAAGGAG 
AACCAGCGCG GCCGCTTCCT GCGCATCCGT CAGACGGTAA ACCGCGGCGG TGGAGGCTTC 
GGCGGGGGAC CCGGGCCCGG CGGCCTGCAG AGCGGCCAGA CCATCGCGCT GCCCGCGCAG 
GGCCTCATCG AGTTCCGCGA CGCGCTCGCC AAGCTCATCG ACGACTACGG GGGCGAGGAT 
GACGAGCTGG CCGGTGGCCC GGGAGGCGGC GCCGGCGGCC CTGGGGGCGG CCTGTACGGA 
GAGCTCCCGG AGGGCACCTC CATCACGGTG GACTCCAAGC GCTTCTTCTT CGACGTCGGC 
TGCAACAAGT ACGGGGTGTT CCTGCGAGTG AGCGAGGTGA AGCCATCCTA CCGCAATGCC 
ATCACCGTGC CCTTCAAGGC CTGGGGCAAG TTTGGAGGCG CCTTCTGTCG GTATGCAGAT 
GAAATGAAAG AAATCCAGGA GCGACAGAGG GATAAGCTTT ACGAGCGACG CGGTGGGGGC 
AGCGGCGGTG GCGACGAGTC CGAGGGTGAG GAAGTGGATG AGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017503.1
CDS	28..975
Translation	MADGDSGSERGGGGPGSFQPAPRGGGGPGGEQETQELASKRLDIQNKRFYLDVKQNAKGRFLKIAEVGAGGSKSRLTLSMAVAAEFRDSLGDFIEHYAQLGPSSPEQLAAGAEEGGGPRRALKSEFLVRENRKYYLDLKENQRGRFLRIRQTVNRGGGGFGGGPGPGGLQSGQTIALPAQGLIEFRDALAKLIDDYGGEDDELAGGPGGGAGGPGGGLYGELPEGTSITVDSKRFFFDVGCNKYGVFLRVSEVKPSYRNAITVPFKAWGKFGGAFCRYADEMKEIQERQRDKLYERRGGGSGGGDESEGEEVDED

Target ORF information:

RefSeq Version	NM_001017503.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus purine rich element binding protein B (Purb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017503.1

ORF Insert Sequence:

ATGGCGGACG GCGACAGCGG CAGCGAGCGC GGTGGCGGCG GGCCCGGCAG CTTCCAGCCC 
GCGCCCCGCG GCGGCGGAGG GCCTGGCGGC GAGCAGGAGA CGCAGGAGCT GGCCTCGAAG 
CGGCTGGACA TCCAGAACAA GCGCTTCTAC CTGGACGTGA AGCAGAACGC CAAGGGCCGC 
TTCCTCAAGA TCGCCGAGGT GGGCGCGGGC GGCTCCAAGA GCCGCCTCAC GCTCTCGATG 
GCGGTGGCCG CCGAGTTCCG CGACTCGCTG GGCGACTTCA TCGAGCACTA CGCGCAGCTG 
GGTCCCAGCA GCCCGGAGCA GCTGGCGGCG GGCGCCGAGG AGGGCGGCGG GCCGCGCCGC 
GCGCTTAAGA GCGAATTCCT GGTGCGCGAG AACCGCAAGT ACTACCTGGA CCTCAAGGAG 
AACCAGCGCG GCCGCTTCCT GCGCATCCGT CAGACGGTAA ACCGCGGCGG TGGAGGCTTC 
GGCGGGGGAC CCGGGCCCGG CGGCCTGCAG AGCGGCCAGA CCATCGCGCT GCCCGCGCAG 
GGCCTCATCG AGTTCCGCGA CGCGCTCGCC AAGCTCATCG ACGACTACGG GGGCGAGGAT 
GACGAGCTGG CCGGTGGCCC GGGAGGCGGC GCCGGCGGCC CTGGGGGCGG CCTGTACGGA 
GAGCTCCCGG AGGGCACCTC CATCACGGTG GACTCCAAGC GCTTCTTCTT CGACGTCGGC 
TGCAACAAGT ACGGGGTGTT CCTGCGAGTG AGCGAGGTGA AGCCATCCTA CCGCAATGCC 
ATCACCGTGC CCTTCAAGGC CTGGGGCAAG TTTGGAGGCG CCTTCTGTCG GTATGCAGAT 
GAAATGAAAG AAATCCAGGA GCGACAGAGG GATAAGCTTT ACGAGCGACG CGGTGGGGGC 
AGCGGCGGTG GCGACGAGTC CGAGGGTGAG GAAGTGGATG AGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

linc-HOXA1 is a noncoding RNA that represses Hoxa1 transcription in cis.
Genes & development27(11)1260-71(2013 Jun)
Maamar H,Cabili MN,Rinn J,Raj A

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

The galectin-3 gene promoter binding proteins in the liver of rats 48-h post-treatment with CCl4.
Gene36746-55(2006 Feb)
Li F,Kato I,Kawaguchi H,Takasawa K,Hibino Y,Hiraga K

A binding site for Pur alpha and Pur beta is structurally unstable and is required for replication in vivo from the rat aldolase B origin.
Biochemical and biophysical research communications340(2)517-25(2006 Feb)
Shimotai Y,Minami H,Saitoh Y,Onodera Y,Mishima Y,Kelm RJ Jr,Tsutsumi K