PURB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PURB
Entrez Gene ID	5814
Full Name	purine rich element binding protein B
Synonyms	PURBETA
General protein information	Preferred Names purine rich element binding protein B Names transcriptional activator protein Pur-beta
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7p13
Summary	This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008].
Disorder MIM:	608887

mRNA and Protein(s)

mRNA	Protein	Name
NM_033224.4	NP_150093.1	transcriptional activator protein Pur-beta
NM_033224.5	NP_150093.1	transcriptional activator protein Pur-beta

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	H2AFV	XP_005642237.1
Danio rerio (zebrafish)	purbb	NP_956841.1
Pan troglodytes (chimpanzee)	PURB	XP_003951050.1
Xenopus tropicalis (tropical clawed frog)	LOC100495008	XP_004912630.1
Homo sapiens (human)	PURB	NP_150093.1
Bos taurus (cattle)	PURB	NP_001193561.1
Mus musculus (house mouse)	Purb	NP_035351.1
Rattus norvegicus (Norway rat)	Purb	NP_001017503.1
Danio rerio (zebrafish)	purba	NP_001153631.1

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of purine-rich element binding protein B as a novel biomarker in acute myelogenous leukemia prognostication.
Kelm RJ Jr, Lamba GS, Levis JE, Holmes CE
Journal of cellular biochemistry119(2)2073-2083(2018 Feb)

Electrostatic and Hydrophobic Interactions Mediate Single-Stranded DNA Recognition and Acta2 Repression by Purine-Rich Element-Binding Protein B.
Rumora AE, Ferris LA, Wheeler TR, Kelm RJ Jr
Biochemistry55(19)2794-805(2016 05)

The Pur?/Pur? single-strand DNA-binding proteins attenuate smooth-muscle actin gene transactivation in myofibroblasts.
Hariharan S, Kelm RJ Jr, Strauch AR
Journal of cellular physiology229(9)1256-71(2014 Sep)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

site-directed mutagenesis revealed several basic amino acid residues in the intermolecular (R267) and intramolecular (K82 and R159) subdomains that are essential for Purbeta transcriptional repressor function in Acta2 promoter-reporter assays. In keeping with their diminished Acta2 repressor activity in fibroblasts, purified Purbeta variants containing an R267A mutation exhibited reduced binding affinity for purine-ric...
Title: Electrostatic and Hydrophobic Interactions Mediate Single-Stranded DNA Recognition and Acta2 Repression by Purine-Rich Element-Binding Protein B.

Suggest dynamic interplay between transcriptional activators Pur-alpha/Pur-beta and repressors in regulating SMalphaA gene output during myofibroblast differentiation.
Title: The Pur?/Pur? single-strand DNA-binding proteins attenuate smooth-muscle actin gene transactivation in myofibroblasts.

Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
Title: Human variation in alcohol response is influenced by variation in neuronal signaling genes.

findings offer insights into mechanism, energetics, and sequence of Purbeta repressor binding to a biologically relevant, contractile phenotype-regulating cis-element while revealing the thermodynamic confines of Purbeta-mediated effects on DNA structure
Title: Mechanism of strand-specific smooth muscle alpha-actin enhancer interaction by purine-rich element binding protein B (Purbeta).

results show that PUR proteins are capable of binding to alpha-MHC mRNA and attenuate its translational efficiency; also show robust expression of PUR proteins in failing hearts where alpha-MHC mRNA levels are suppressed
Title: Single-stranded DNA-binding proteins PURalpha and PURbeta bind to a purine-rich negative regulatory element of the alpha-myosin heavy chain gene and control transcriptional and translational regulation of the gene expression. Implications in the repression of alpha-myosin heavy chain during heart failure.

The following PURB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PURB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12284	NM_033224.5 Latest version!	Homo sapiens purine rich element binding protein B (PURB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
View Old Accession Versions >>
OHu12284	NM_033224.4	Homo sapiens purine rich element binding protein B (PURB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PURB ( NM_033224.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_150093.1 Homo sapiens purine rich element binding protein B (PURB), mRNA.

Price & Availability↑

CloneID	OHu12284
Clone ID Related Accession (Same CDS sequence)	NM_033224.4 , NM_033224.5
Accession Version	NM_033224.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	transcriptional activator protein Pur-beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR005180.1, AY039216.1, AC004854.3, AK054977.1 and AI042138.1. On Sep 6, 2013 this sequence version replaced NM_033224.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGGCGGACG GCGACAGCGG CAGCGAGCGC GGCGGCGGCG GTGGGCCGTG CGGGTTCCAG 
CCCGCGTCCC GCGGCGGCGG CGAGCAAGAG ACGCAGGAGC TGGCCTCGAA GCGGCTGGAC 
ATCCAGAACA AGCGCTTCTA CTTAGATGTG AAGCAGAACG CCAAGGGCCG CTTCCTCAAG 
ATCGCCGAGG TGGGCGCGGG CGGTTCCAAG AGCCGCCTCA CGCTGTCCAT GGCGGTGGCC 
GCCGAGTTCC GCGACTCGCT GGGCGACTTC ATAGAACACT ACGCGCAGCT GGGCCCTAGC 
AGCCCCGAGC AGCTGGCGGC TGGCGCCGAG GAGGGCGGCG GGCCGCGGCG CGCGCTCAAG 
AGCGAATTCT TGGTGCGTGA GAACCGCAAG TACTACCTGG ACCTCAAGGA GAACCAGCGC 
GGCCGCTTCC TGCGCATCCG CCAAACGGTC AACCGCGGCG GTGGCGGCTT CGGCGCGGGC 
CCCGGGCCGG GCGGCTTGCA GAGCGGCCAG ACCATCGCGC TGCCTGCGCA GGGCCTCATC 
GAGTTCCGCG ACGCGCTGGC GAAGCTCATA GACGACTACG GAGGCGAGGA CGACGAGCTG 
GCAGGCGGCC CGGGAGGCGG CGCCGGGGGC CCAGGGGGCG GCCTGTATGG AGAGCTCCCG 
GAGGGCACCT CCATCACCGT GGACTCCAAG CGCTTCTTCT TCGATGTGGG CTGCAACAAA 
TACGGGGTGT TTCTGCGAGT GAGCGAGGTG AAGCCGTCCT ACCGCAATGC CATCACCGTA 
CCCTTCAAAG CCTGGGGCAA GTTCGGAGGC GCCTTTTGCC GGTATGCGGA TGAGATGAAA 
GAAATCCAGG AACGACAGAG GGATAAGCTT TATGAGCGAC GTGGTGGGGG CAGCGGCGGC 
GGCGAAGAGT CAGAGGGTGA GGAGGTGGAT GAGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_150093.1
CDS	38..976
Translation	MADGDSGSERGGGGGPCGFQPASRGGGEQETQELASKRLDIQNKRFYLDVKQNAKGRFLKIAEVGAGGSKSRLTLSMAVAAEFRDSLGDFIEHYAQLGPSSPEQLAAGAEEGGGPRRALKSEFLVRENRKYYLDLKENQRGRFLRIRQTVNRGGGGFGAGPGPGGLQSGQTIALPAQGLIEFRDALAKLIDDYGGEDDELAGGPGGGAGGPGGGLYGELPEGTSITVDSKRFFFDVGCNKYGVFLRVSEVKPSYRNAITVPFKAWGKFGGAFCRYADEMKEIQERQRDKLYERRGGGSGGGEESEGEEVDED

Target ORF information:

RefSeq Version	NM_033224.4
Organism	Homo sapiens(human)
Definition	Homo sapiens purine rich element binding protein B (PURB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033224.4

ORF Insert Sequence:

ATGGCGGACG GCGACAGCGG CAGCGAGCGC GGCGGCGGCG GTGGGCCGTG CGGGTTCCAG 
CCCGCGTCCC GCGGCGGCGG CGAGCAAGAG ACGCAGGAGC TGGCCTCGAA GCGGCTGGAC 
ATCCAGAACA AGCGCTTCTA CTTAGATGTG AAGCAGAACG CCAAGGGCCG CTTCCTCAAG 
ATCGCCGAGG TGGGCGCGGG CGGTTCCAAG AGCCGCCTCA CGCTGTCCAT GGCGGTGGCC 
GCCGAGTTCC GCGACTCGCT GGGCGACTTC ATAGAACACT ACGCGCAGCT GGGCCCTAGC 
AGCCCCGAGC AGCTGGCGGC TGGCGCCGAG GAGGGCGGCG GGCCGCGGCG CGCGCTCAAG 
AGCGAATTCT TGGTGCGTGA GAACCGCAAG TACTACCTGG ACCTCAAGGA GAACCAGCGC 
GGCCGCTTCC TGCGCATCCG CCAAACGGTC AACCGCGGCG GTGGCGGCTT CGGCGCGGGC 
CCCGGGCCGG GCGGCTTGCA GAGCGGCCAG ACCATCGCGC TGCCTGCGCA GGGCCTCATC 
GAGTTCCGCG ACGCGCTGGC GAAGCTCATA GACGACTACG GAGGCGAGGA CGACGAGCTG 
GCAGGCGGCC CGGGAGGCGG CGCCGGGGGC CCAGGGGGCG GCCTGTATGG AGAGCTCCCG 
GAGGGCACCT CCATCACCGT GGACTCCAAG CGCTTCTTCT TCGATGTGGG CTGCAACAAA 
TACGGGGTGT TTCTGCGAGT GAGCGAGGTG AAGCCGTCCT ACCGCAATGC CATCACCGTA 
CCCTTCAAAG CCTGGGGCAA GTTCGGAGGC GCCTTTTGCC GGTATGCGGA TGAGATGAAA 
GAAATCCAGG AACGACAGAG GGATAAGCTT TATGAGCGAC GTGGTGGGGG CAGCGGCGGC 
GGCGAAGAGT CAGAGGGTGA GGAGGTGGAT GAGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PURB ( NM_033224.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_150093.1 Homo sapiens purine rich element binding protein B (PURB), mRNA.

Price & Availability↑

CloneID	OHu12284
Clone ID Related Accession (Same CDS sequence)	NM_033224.4 , NM_033224.5
Accession Version	NM_033224.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	transcriptional activator protein Pur-beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004854.3. On May 17, 2019 this sequence version replaced NM_033224.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: SRR1803611.133393.1, SRR1803615.68634.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000395699.5/ ENSP00000379051.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG GCGACAGCGG CAGCGAGCGC GGCGGCGGCG GTGGGCCGTG CGGGTTCCAG 
CCCGCGTCCC GCGGCGGCGG CGAGCAAGAG ACGCAGGAGC TGGCCTCGAA GCGGCTGGAC 
ATCCAGAACA AGCGCTTCTA CTTAGATGTG AAGCAGAACG CCAAGGGCCG CTTCCTCAAG 
ATCGCCGAGG TGGGCGCGGG CGGTTCCAAG AGCCGCCTCA CGCTGTCCAT GGCGGTGGCC 
GCCGAGTTCC GCGACTCGCT GGGCGACTTC ATAGAACACT ACGCGCAGCT GGGCCCTAGC 
AGCCCCGAGC AGCTGGCGGC TGGCGCCGAG GAGGGCGGCG GGCCGCGGCG CGCGCTCAAG 
AGCGAATTCT TGGTGCGTGA GAACCGCAAG TACTACCTGG ACCTCAAGGA GAACCAGCGC 
GGCCGCTTCC TGCGCATCCG CCAAACGGTC AACCGCGGCG GTGGCGGCTT CGGCGCGGGC 
CCCGGGCCGG GCGGCTTGCA GAGCGGCCAG ACCATCGCGC TGCCTGCGCA GGGCCTCATC 
GAGTTCCGCG ACGCGCTGGC GAAGCTCATA GACGACTACG GAGGCGAGGA CGACGAGCTG 
GCAGGCGGCC CGGGAGGCGG CGCCGGGGGC CCAGGGGGCG GCCTGTATGG AGAGCTCCCG 
GAGGGCACCT CCATCACCGT GGACTCCAAG CGCTTCTTCT TCGATGTGGG CTGCAACAAA 
TACGGGGTGT TTCTGCGAGT GAGCGAGGTG AAGCCGTCCT ACCGCAATGC CATCACCGTA 
CCCTTCAAAG CCTGGGGCAA GTTCGGAGGC GCCTTTTGCC GGTATGCGGA TGAGATGAAA 
GAAATCCAGG AACGACAGAG GGATAAGCTT TATGAGCGAC GTGGTGGGGG CAGCGGCGGC 
GGCGAAGAGT CAGAGGGTGA GGAGGTGGAT GAGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_150093.1
CDS	183..1121
Translation	MADGDSGSERGGGGGPCGFQPASRGGGEQETQELASKRLDIQNKRFYLDVKQNAKGRFLKIAEVGAGGSKSRLTLSMAVAAEFRDSLGDFIEHYAQLGPSSPEQLAAGAEEGGGPRRALKSEFLVRENRKYYLDLKENQRGRFLRIRQTVNRGGGGFGAGPGPGGLQSGQTIALPAQGLIEFRDALAKLIDDYGGEDDELAGGPGGGAGGPGGGLYGELPEGTSITVDSKRFFFDVGCNKYGVFLRVSEVKPSYRNAITVPFKAWGKFGGAFCRYADEMKEIQERQRDKLYERRGGGSGGGEESEGEEVDED

Target ORF information:

RefSeq Version	NM_033224.5
Organism	Homo sapiens(human)
Definition	Homo sapiens purine rich element binding protein B (PURB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033224.5

ORF Insert Sequence:

ATGGCGGACG GCGACAGCGG CAGCGAGCGC GGCGGCGGCG GTGGGCCGTG CGGGTTCCAG 
CCCGCGTCCC GCGGCGGCGG CGAGCAAGAG ACGCAGGAGC TGGCCTCGAA GCGGCTGGAC 
ATCCAGAACA AGCGCTTCTA CTTAGATGTG AAGCAGAACG CCAAGGGCCG CTTCCTCAAG 
ATCGCCGAGG TGGGCGCGGG CGGTTCCAAG AGCCGCCTCA CGCTGTCCAT GGCGGTGGCC 
GCCGAGTTCC GCGACTCGCT GGGCGACTTC ATAGAACACT ACGCGCAGCT GGGCCCTAGC 
AGCCCCGAGC AGCTGGCGGC TGGCGCCGAG GAGGGCGGCG GGCCGCGGCG CGCGCTCAAG 
AGCGAATTCT TGGTGCGTGA GAACCGCAAG TACTACCTGG ACCTCAAGGA GAACCAGCGC 
GGCCGCTTCC TGCGCATCCG CCAAACGGTC AACCGCGGCG GTGGCGGCTT CGGCGCGGGC 
CCCGGGCCGG GCGGCTTGCA GAGCGGCCAG ACCATCGCGC TGCCTGCGCA GGGCCTCATC 
GAGTTCCGCG ACGCGCTGGC GAAGCTCATA GACGACTACG GAGGCGAGGA CGACGAGCTG 
GCAGGCGGCC CGGGAGGCGG CGCCGGGGGC CCAGGGGGCG GCCTGTATGG AGAGCTCCCG 
GAGGGCACCT CCATCACCGT GGACTCCAAG CGCTTCTTCT TCGATGTGGG CTGCAACAAA 
TACGGGGTGT TTCTGCGAGT GAGCGAGGTG AAGCCGTCCT ACCGCAATGC CATCACCGTA 
CCCTTCAAAG CCTGGGGCAA GTTCGGAGGC GCCTTTTGCC GGTATGCGGA TGAGATGAAA 
GAAATCCAGG AACGACAGAG GGATAAGCTT TATGAGCGAC GTGGTGGGGG CAGCGGCGGC 
GGCGAAGAGT CAGAGGGTGA GGAGGTGGAT GAGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of purine-rich element binding protein B as a novel biomarker in acute myelogenous leukemia prognostication.
Journal of cellular biochemistry119(2)2073-2083(2018 Feb)
Kelm RJ Jr,Lamba GS,Levis JE,Holmes CE

Electrostatic and Hydrophobic Interactions Mediate Single-Stranded DNA Recognition and Acta2 Repression by Purine-Rich Element-Binding Protein B.
Biochemistry55(19)2794-805(2016 05)
Rumora AE,Ferris LA,Wheeler TR,Kelm RJ Jr

The Pur?/Pur? single-strand DNA-binding proteins attenuate smooth-muscle actin gene transactivation in myofibroblasts.
Journal of cellular physiology229(9)1256-71(2014 Sep)
Hariharan S,Kelm RJ Jr,Strauch AR

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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PURB cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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