Purb cDNA ORF clone, Mus musculus(house mouse)

Electrostatic and Hydrophobic Interactions Mediate Single-Stranded DNA Recognition and Acta2 Repression by Purine-Rich Element-Binding Protein B.
Rumora AE, Ferris LA, Wheeler TR, Kelm RJ Jr
Biochemistry55(19)2794-805(2016 05)

Structural basis of multisite single-stranded DNA recognition and ACTA2 repression by purine-rich element binding protein B (Pur?).
Rumora AE, Wang SX, Ferris LA, Everse SJ, Kelm RJ Jr
Biochemistry52(26)4439-50(2013 Jul)

linc-HOXA1 is a noncoding RNA that represses Hoxa1 transcription in cis.
Maamar H, Cabili MN, Rinn J, Raj A
Genes & development27(11)1260-71(2013 Jun)

Isolation and characterization of the core single-stranded DNA-binding domain of purine-rich element binding protein B (Pur?).
Rumora AE, Steere AN, Ramsey JE, Knapp AM, Ballif BA, Kelm RJ Jr
Biochemical and biophysical research communications400(3)340-5(2010 Sep)

TEAD-1 overexpression in the mouse heart promotes an age-dependent heart dysfunction.
Tsika RW, Ma L, Kehat I, Schramm C, Simmer G, Morgan B, Fine DM, Hanft LM, McDonald KS, Molkentin JD, Krenz M, Yang S, Ji J
The Journal of biological chemistry285(18)13721-35(2010 Apr)

site-directed mutagenesis revealed several basic amino acid residues in the intermolecular (R267) and intramolecular (K82 and R159) subdomains that are essential for Purbeta transcriptional repressor function in Acta2 promoter-reporter assays. In keeping with their diminished Acta2 repressor activity in fibroblasts, purified Purbeta variants containing an R267A mutation exhibited reduced binding affinity for purine-ric...
Title: Electrostatic and Hydrophobic Interactions Mediate Single-Stranded DNA Recognition and Acta2 Repression by Purine-Rich Element-Binding Protein B.

This structural arrangement suggests that the cooperative assembly of the dimeric Purbeta repressor on the sense strand of the ACTA2 enhancer is dictated by the association of each subdomain with distinct purine-rich binding sites within the enhancer.
Title: Structural basis of multisite single-stranded DNA recognition and ACTA2 repression by purine-rich element binding protein B (Pur?).

These findings suggest that the highly conserved modular repeats of Purbeta fold to form a core functional domain, which mediates the specific and high affinity binding of the protein to single-stranded DNA.
Title: Isolation and characterization of the core single-stranded DNA-binding domain of purine-rich element binding protein B (Pur?).

Overexpression of Purbeta, but not Puralpha, significantly downregulated GnRH1 mRNA levels in transiently transfected GT1-7 cells, suggesting that Purbeta acts as a repressor of GnRH1 gene transcription.
Title: Regulation of gonadotropin-releasing hormone-1 gene transcription by members of the purine-rich element-binding protein family.

Activation of the normally silent SM alpha A gene in reprogrammed adult cardiomyocytes is linked to elevated interaction of serum response factor with Pur-alpha/Pur-beta.
Title: Serum response factor neutralizes Pur alpha- and Pur beta-mediated repression of the fetal vascular smooth muscle alpha-actin gene in stressed adult cardiomyocytes.