Med1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Med1
Entrez Gene ID	497991
Full Name	mediator complex subunit 1
Synonyms	RGD1559552
General protein information	Preferred Names mediator complex subunit 1 Names mediator of RNA polymerase II transcription subunit 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q31

mRNA and Protein(s)

mRNA	Protein	Name
NM_001134361.1	NP_001127833.1	mediator of RNA polymerase II transcription subunit 1
XM_006247398.3	XP_006247460.1	mediator of RNA polymerase II transcription subunit 1 isoform X1
XM_006247399.3	XP_006247461.1	mediator of RNA polymerase II transcription subunit 1 isoform X2
XM_006247401.3	XP_006247463.1	mediator of RNA polymerase II transcription subunit 1 isoform X4
XM_017597471.1	XP_017452960.1	mediator of RNA polymerase II transcription subunit 1 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MED1	XP_001172406.1
Canis lupus familiaris (dog)	MED1	XP_864009.2
Rattus norvegicus (Norway rat)	Med1	NP_001127833.1
Gallus gallus (chicken)	MED1	XP_418125.3
Bos taurus (cattle)	MED1	NP_001192805.1
Mus musculus (house mouse)	Med1	NP_001073587.1
Homo sapiens (human)	MED1	NP_004765.2
Xenopus tropicalis (tropical clawed frog)	med1	NP_001090727.1

Gene Ontology
Bibliography

Related articles in PubMed

Cardiomyocyte-Specific Ablation of Med1 Subunit of the Mediator Complex Causes Lethal Dilated Cardiomyopathy in Mice.
Jia Y, Chang HC, Schipma MJ, Liu J, Shete V, Liu N, Sato T, Thorp EB, Barger PM, Zhu YJ, Viswakarma N, Kanwar YS, Ardehali H, Thimmapaya B, Reddy JK
PloS one11(8)e0160755(2016)

CCAR1/CoCoA pair-mediated recruitment of the Mediator defines a novel pathway for GATA1 function.
Mizuta S, Minami T, Fujita H, Kaminaga C, Matsui K, Ishino R, Fujita A, Oda K, Kawai A, Hasegawa N, Urahama N, Roeder RG, Ito M
Genes to cells : devoted to molecular & cellular mechanisms19(1)28-51(2014 Jan)

The Mediator complex and transcription regulation.
Poss ZC, Ebmeier CC, Taatjes DJ
Critical reviews in biochemistry and molecular biology48(6)575-608(2013 Nov-Dec)

Defective transcription initiation causes postnatal growth failure in a mouse model of nucleotide excision repair (NER) progeria.
Kamileri I, Karakasilioti I, Sideri A, Kosteas T, Tatarakis A, Talianidis I, Garinis GA
Proceedings of the National Academy of Sciences of the United States of America109(8)2995-3000(2012 Feb)

Specific erythroid-lineage defect in mice conditionally deficient for Mediator subunit Med1.
Stumpf M, Yue X, Schmitz S, Luche H, Reddy JK, Borggrefe T
Proceedings of the National Academy of Sciences of the United States of America107(50)21541-6(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Med1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Med1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa33749	XM_006247401.3 Latest version!	Rattus norvegicus mediator complex subunit 1 (Med1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa57441	XM_017597471.1 Latest version!	Rattus norvegicus mediator complex subunit 1 (Med1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa02628	NM_001134361.1 Latest version!	Rattus norvegicus mediator complex subunit 1 (Med1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa02628	XM_006247399.3 Latest version!	Rattus norvegicus mediator complex subunit 1 (Med1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa33747	XM_006247398.3 Latest version!	Rattus norvegicus mediator complex subunit 1 (Med1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Med1 ( XM_006247401.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247463.1 Rattus norvegicus mediator complex subunit 1 (Med1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa33749
Clone ID Related Accession (Same CDS sequence)	XM_006247401.3
Accession Version	XM_006247401.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mediator of RNA polymerase II transcription subunit 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247401.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCTC AGGGGGAAAC CGAGGAGTCA GAGAAGCTGA GTAAGATGAG TTCTCTCTTG 
GACCGGCTCC ATGCAAAATT TAACCAAAGT AGACCTTGGA GTGAGACCAT TAAGCTTGTG 
CGTCAAGTCA TGGAGAAGAG GGTCGTAATG AGTTCTGGAG GACATCAGCA TTTGGTCAGC 
TGTTTGGAGA CATTACAGAA GGCTCTCAAA GTAACATCTT TGCCAGCAAT GACTGATCGT 
TTGGAATCCA TAGCCAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA 
TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGACCCTGC AGGACAGCTT 
TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCAGA GCTTGTGCAG 
CAGTTAAGGG AAAAGAATTT TGATGAGTTT TCTAAGCATC TTAAGGGTCT TGTTAATCTG 
TATAACCTTC CAGGAGACAA CAAACTGAAG ACTAAGATGT ATCTGGCTCT CCAGTCCTTA 
GAACAGGACC TTTCTAAAAT GGCAATTATG TACTGGAAGG CAACTAATGC TACTCCCTTG 
GATAAGATTC TTCACGGAAG TGTTGGCTAT CTCACTCCAC GGAGCGGGGG TCATTTAATG 
AACATGAAAT ACTATGCCTC TCCGTCTGAC CTGCTGCATG ATACAACTGC CTCTCCTATC 
ACTTTGCATG AAAAGAATGT TCCTCGGTCT TTGGGAATGA ATGCCTCAGT GACAATTGAA 
GGAACCTCTG CTATGTACAA ACTCCCAATT GCCCCATTAA TTATGGGGTC ACACCCAGCT 
GACAACAAAT GGACCCCTTC TTTCTCTGCA GTCACTAGTG CCAACAGTGT TGATCTTCCT 
GCGTGTTTCT TCTTGAAATT TCCCCAGCCA ATCCCAGTAT CTAAAGCATT TGTTCAGAAA 
CTGCAAAATT GCACGGGAAT CCCGTTGTTT GAGACTCCGC CTACTTACCT GCCCCTCTAT 
GAACTCATCA CTCAGTTTGA GCTGTCAAAG GATCCTGACC CTTTACCTTT GAATCACAAC 
ATGCGATTTT ACGCTGCTCT TCCAGGTCAG CAGCACTGCT ATTTTCTCAA TAAAGATGCT 
CCTCTTCCTG ATGGCCAAAG CCTGCAGGGA ACACTGGTCA GCAAAATCAC CTTCCAGCAC 
CCTGGCCGAG TTCCTCTTAT CTTGAATATG ATCAGACACC AAGTGGCCTA TAACACCCTA 
ATTGGAAGCT GTGTTAAAAG AACTATTTTA AAAGAAGATT CTCCTGGGCT CCTCCAATTT 
GAAGTGTGTC CTCTTTCAGA ATCTCGATTC AGTGTATCTT TTCAGCACCC TGTGAATGAC 
TCCCTTGTGT GTGTGGTGAT GGACGTGCAA GACTCAACAC ATGTGAGCTG TAAACTCTAC 
AAGGGGCTAT CAGATGCACT GATCTGTACA GACGACTTCA TTGCCAAAGT TGTTCAAAGA 
TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AGGCTGAAAC CATACAGGCT 
GACACGCCAG CACTGTCCCT CATTGCAGAG ACAGTCGAAG ACATGGTGAA AAAGAACCTG 
CCCCCGGCTA GCAGCCCAGG ATCAAAGAAT CCCGAGTTAG GATCTGGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247463.1
CDS	218..1888
Translation	MKAQGETEESEKLSKMSSLLDRLHAKFNQSRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNATPLDKILHGSVGYLTPRSGGHLMNMKYYASPSDLLHDTTASPITLHEKNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPADNKWTPSFSAVTSANSVDLPACFFLKFPQPIPVSKAFVQKLQNCTGIPLFETPPTYLPLYELITQFELSKDPDPLPLNHNMRFYAALPGQQHCYFLNKDAPLPDGQSLQGTLVSKITFQHPGRVPLILNMIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGSKNPELGSG

Target ORF information:

RefSeq Version	XM_006247401.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mediator complex subunit 1 (Med1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247401.3

ORF Insert Sequence:

ATGAAGGCTC AGGGGGAAAC CGAGGAGTCA GAGAAGCTGA GTAAGATGAG TTCTCTCTTG 
GACCGGCTCC ATGCAAAATT TAACCAAAGT AGACCTTGGA GTGAGACCAT TAAGCTTGTG 
CGTCAAGTCA TGGAGAAGAG GGTCGTAATG AGTTCTGGAG GACATCAGCA TTTGGTCAGC 
TGTTTGGAGA CATTACAGAA GGCTCTCAAA GTAACATCTT TGCCAGCAAT GACTGATCGT 
TTGGAATCCA TAGCCAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA 
TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGACCCTGC AGGACAGCTT 
TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCAGA GCTTGTGCAG 
CAGTTAAGGG AAAAGAATTT TGATGAGTTT TCTAAGCATC TTAAGGGTCT TGTTAATCTG 
TATAACCTTC CAGGAGACAA CAAACTGAAG ACTAAGATGT ATCTGGCTCT CCAGTCCTTA 
GAACAGGACC TTTCTAAAAT GGCAATTATG TACTGGAAGG CAACTAATGC TACTCCCTTG 
GATAAGATTC TTCACGGAAG TGTTGGCTAT CTCACTCCAC GGAGCGGGGG TCATTTAATG 
AACATGAAAT ACTATGCCTC TCCGTCTGAC CTGCTGCATG ATACAACTGC CTCTCCTATC 
ACTTTGCATG AAAAGAATGT TCCTCGGTCT TTGGGAATGA ATGCCTCAGT GACAATTGAA 
GGAACCTCTG CTATGTACAA ACTCCCAATT GCCCCATTAA TTATGGGGTC ACACCCAGCT 
GACAACAAAT GGACCCCTTC TTTCTCTGCA GTCACTAGTG CCAACAGTGT TGATCTTCCT 
GCGTGTTTCT TCTTGAAATT TCCCCAGCCA ATCCCAGTAT CTAAAGCATT TGTTCAGAAA 
CTGCAAAATT GCACGGGAAT CCCGTTGTTT GAGACTCCGC CTACTTACCT GCCCCTCTAT 
GAACTCATCA CTCAGTTTGA GCTGTCAAAG GATCCTGACC CTTTACCTTT GAATCACAAC 
ATGCGATTTT ACGCTGCTCT TCCAGGTCAG CAGCACTGCT ATTTTCTCAA TAAAGATGCT 
CCTCTTCCTG ATGGCCAAAG CCTGCAGGGA ACACTGGTCA GCAAAATCAC CTTCCAGCAC 
CCTGGCCGAG TTCCTCTTAT CTTGAATATG ATCAGACACC AAGTGGCCTA TAACACCCTA 
ATTGGAAGCT GTGTTAAAAG AACTATTTTA AAAGAAGATT CTCCTGGGCT CCTCCAATTT 
GAAGTGTGTC CTCTTTCAGA ATCTCGATTC AGTGTATCTT TTCAGCACCC TGTGAATGAC 
TCCCTTGTGT GTGTGGTGAT GGACGTGCAA GACTCAACAC ATGTGAGCTG TAAACTCTAC 
AAGGGGCTAT CAGATGCACT GATCTGTACA GACGACTTCA TTGCCAAAGT TGTTCAAAGA 
TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AGGCTGAAAC CATACAGGCT 
GACACGCCAG CACTGTCCCT CATTGCAGAG ACAGTCGAAG ACATGGTGAA AAAGAACCTG 
CCCCCGGCTA GCAGCCCAGG ATCAAAGAAT CCCGAGTTAG GATCTGGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med1 ( XM_017597471.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452960.1 Rattus norvegicus mediator complex subunit 1 (Med1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa57441
Clone ID Related Accession (Same CDS sequence)	XM_017597471.1
Accession Version	XM_017597471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mediator of RNA polymerase II transcription subunit 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCTGG CTCTCCAGTC CTTAGAACAG GACCTTTCTA AAATGGCAAT TATGTACTGG 
AAGGCAACTA ATGCTACTCC CTTGGATAAG ATTCTTCACG GAAGTGTTGG CTATCTCACT 
CCACGGAGCG GGGGTCATTT AATGAACATG AAATACTATG CCTCTCCGTC TGACCTGCTG 
CATGATACAA CTGCCTCTCC TATCACTTTG CATGAAAAGA ATGTTCCTCG GTCTTTGGGA 
ATGAATGCCT CAGTGACAAT TGAAGGAACC TCTGCTATGT ACAAACTCCC AATTGCCCCA 
TTAATTATGG GGTCACACCC AGCTGACAAC AAATGGACCC CTTCTTTCTC TGCAGTCACT 
AGTGCCAACA GTGTTGATCT TCCTGCGTGT TTCTTCTTGA AATTTCCCCA GCCAATCCCA 
GTATCTAAAG CATTTGTTCA GAAACTGCAA AATTGCACGG GAATCCCGTT GTTTGAGACT 
CCGCCTACTT ACCTGCCCCT CTATGAACTC ATCACTCAGT TTGAGCTGTC AAAGGATCCT 
GACCCTTTAC CTTTGAATCA CAACATGCGA TTTTACGCTG CTCTTCCAGG TCAGCAGCAC 
TGCTATTTTC TCAATAAAGA TGCTCCTCTT CCTGATGGCC AAAGCCTGCA GGGAACACTG 
GTCAGCAAAA TCACCTTCCA GCACCCTGGC CGAGTTCCTC TTATCTTGAA TATGATCAGA 
CACCAAGTGG CCTATAACAC CCTAATTGGA AGCTGTGTTA AAAGAACTAT TTTAAAAGAA 
GATTCTCCTG GGCTCCTCCA ATTTGAAGTG TGTCCTCTTT CAGAATCTCG ATTCAGTGTA 
TCTTTTCAGC ACCCTGTGAA TGACTCCCTT GTGTGTGTGG TGATGGACGT GCAAGACTCA 
ACACATGTGA GCTGTAAACT CTACAAGGGG CTATCAGATG CACTGATCTG TACAGACGAC 
TTCATTGCCA AAGTTGTTCA AAGATGTATG TCCATCCCTG TGACGATGAG GGCTATTCGG 
AGGAAGGCTG AAACCATACA GGCTGACACG CCAGCACTGT CCCTCATTGC AGAGACAGTC 
GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA 
GGCAGCAACC CAATGAGCGG TACCACCACA CCAACCAACA CCTTTCCGGG GGGTCCCATT 
ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG 
GAGGACTTCA GCAAGGTGTC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG 
AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC 
TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAC 
CCTGCCCAAG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCCTCT 
TCCGGATCAC CCCGGATGGA AATGTGCTCG GGGAGCAACA AGACCAAGAA AAATAAGTCA 
TCAAGAGTAC CACCGGACAA ACCCAAGCAC CAGACCGAAG ATGATTTTCA GAGGGAGCTC 
TTTTCAATGG ATGTCGACTC ACAGAACCCT ATGTTCGATG TCAGCATGAC AGCTGACGCG 
CTGGATACAC CTCATATCAC TCCAGCTCCA AGCCAGTGTA GTACGCCCCC AACAACGTAC 
CCACAGCCAG TGTCTCACCC CCAGCCCAGT ATTCAAAGGA TGGGCCGACT GTCCAGTTCA 
GACAGCATTG GCCCAGATGT AACTGATATT CTTTCAGACA TTGCAGAAGA AGCTTCAAAG 
CTTCCCAGCA CTAGTGATGA TTGCCCACCC ATTGGTACCC CAGTTCGAGA TTCCTCGAGT 
TCTGGGCATT CTCAGAGTGC CCTCTTTGAC TCTGATGTCT TTCAGACTAA TAACAATGAA 
AATCCATACA CTGACCCGGC TGACCTTATT GCAGATGCTG CTGGGAGCCC CAGTAGTGAT 
TCTCCTACCA ATCATTTTTT CCCTGATGGA GTAGATTTCA ATCCTGATTT GTTGAACAGC 
CAAAGCCAAA GTGGTTTTGG AGAGGAGTAT TTTGATGAAA GTAGTCAGAG TGGGGATAAT 
GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGATGCC AATGCTCGGA 
GGTGACAATG GGGAGTCGAA ATTGAAGGGC AGCAGCCAAG CTGACACAGT TGACTTCAGT 
ATTCTGTCAG TAGCTGGTAA GGCTTTGGGT GCAGCAGACC TTCTGGAGCA CCACAGTGGT 
AGTCAGAGTC CTCTGCTGAC CACTGGAGAA TTAGGGAAAG AAAAAACTCA AAAGAGGGTG 
AAGGAAGGCA ACGGTATGGG CGCTAGCAGT GGGTCAGGCC CGGGGTCAGA CAGCAAGCCA 
GGCAAGCGCA GCCGCACACC TTCCAATGAT GGGAAGAGCA AAGATAAGCC TCCAAAGCGA 
AAGAAGGCAG ACACGGAGGG AAAATCCCCA TCTCACAGTT CTAATAGACC TTTCACCCCA 
CCCACCAGTA CAGGTGGGTC CAAATCCCCT GGCAGTTCAG GACGATGTCA GACGCCCCCT 
GGTGTCGCCA CACCGCCCAT TCCCAAGATC ACCATTCAGA TTCCTAAAGG AACAGTGATG 
GTGGGCAAGC CTTCCTCGCA CAGTCAGTAC TCTAGCAGTG GTTCTGTGTC TTCCTCTGGC 
AGCAAAAGCC ACCACAGTCA TTCTTCCTCC TCCTCTTCCT TATCTTCTGC TTCCACCTCA 
GGCAAGATGA AAAGCAGTAA ATCAGAAGGC TCCTCAAGTG CCAAGCTCAG TGGCAGTATG 
TATTCTAGCC AAGGGTCTTC TGGATCCAGC CAGTCCAAAA ATTCATCTCA GACTGGGGGG 
AAGCCAGGCT CCTCTCCCAT TACCAAACAT GGACTGAGTA GTGGGTCTAG CAGCACCAAG 
ATGAAACCTC AAGGCAAGCC ATCCTCACTT ATGAATCCTT CTATAAGTAA ACCAAACATA 
TCCCCTTCCC ATTCAAGGCC TCCTGGAGGC TCAGATAAGC TTGCCTCTCC AATGAAGCCT 
GTTCCCGGGA CCCCCCCATC CTCTAAAGCC AAGTCCCCTA TCAGTTCAGG TTCTGGTAGC 
TCTCATGTGT CAGGAACTAG TTCAAGCTCT GGTATGAAGT CTTCTTCAGG GTCAGGATCC 
TCAGGCTCAT TGTCTCAAAA AACTCCCCCA GCATCTAATT CTTGTACACC ATCTTCCTCC 
TCATTTTCCT CAAGTGGTTC TTCCATGTCA TCCTCTCAGA ATCAACATGG GAGTTCCAAA 
GGGAAATCTC CCAGTAGGAA TAAGAAGCCT TCTTTGACAG CTGTCATAGA TAAATTGAAG 
CATGGGGTGG TTACCAGTGG GCCTGGGGGT GAGGATCCAA TAGACAGTCA GATGGGTGCA 
AGCACAAATT CCTCTAACCA TCCCATGTCC TCCAAACATA ACATGTCAGG AGGAGAATTC 
CAGAGCAAAC GTGAGAAAAG CGATAAAGAC AAATCAAAGG TTTCTACTTC TGGAGGATCA 
GTTGATTCCT CTAAGAAGAC TTCAGAGTCA AAAAATGTGG GGAGCACGGG TGTGGCAAAA 
ATTATTATCA GCAAGCACGA TGGAGGCTCC CCGAGCATCA AAGCCAAAGT GACTCTACAG 
AAACCTGGAG ATAGTGGTGG AGAAGGGCTC AGGCCACAGA TCGCCTCTTC AAAAAACTAT 
GGCTCTCCAT TGATCAGTGG TTCTACTCCA AAGCATGAAC GGGGTTCTCC CAGCCACAGT 
AAGTCGCCAG CATATACACC TCAGAATGTG GACAGTGAAA GTGAGTCAGG CTCCTCCATA 
GCAGAGAGAT CCTACCAGAA CAGCCCCAGC TCAGATGATG GTGTCCGGCC ACTCCCAGAA 
TACAGCACAG AGAAGCATAA GAAGCACAAA AAGGAAAAGA AAAAAGTAAA AGATAAAGAT 
AGAGACCGGG ACAGAGACAA AGACAGAGAC AAGAAAAAAT CTCACAGCAT CAAGCCAGAG 
AATTGGTCTA AATCCCCCAT TTCTTCAGAT CAGACTTCAT CTATGACAAA TAACCCTATC 
TTATCTGCAG ACAGGCCTTC CAGGCTCAGC CCTGACTTTA TGATTGGGGA GGAAGATGAT 
GATCTTATGG ATGTGGCTCT GATTGGCAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452960.1
CDS	193..4425
Translation	MYLALQSLEQDLSKMAIMYWKATNATPLDKILHGSVGYLTPRSGGHLMNMKYYASPSDLLHDTTASPITLHEKNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPADNKWTPSFSAVTSANSVDLPACFFLKFPQPIPVSKAFVQKLQNCTGIPLFETPPTYLPLYELITQFELSKDPDPLPLNHNMRFYAALPGQQHCYFLNKDAPLPDGQSLQGTLVSKITFQHPGRVPLILNMIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGSNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMCSGSNKTKKNKSSRVPPDKPKHQTEDDFQRELFSMDVDSQNPMFDVSMTADALDTPHITPAPSQCSTPPTTYPQPVSHPQPSIQRMGRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPPIGTPVRDSSSSGHSQSALFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGMPMLGGDNGESKLKGSSQADTVDFSILSVAGKALGAADLLEHHSGSQSPLLTTGELGKEKTQKRVKEGNGMGASSGSGPGSDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSNRPFTPPTSTGGSKSPGSSGRCQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYSSSGSVSSSGSKSHHSHSSSSSSLSSASTSGKMKSSKSEGSSSAKLSGSMYSSQGSSGSSQSKNSSQTGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSISKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGSSHVSGTSSSSGMKSSSGSGSSGSLSQKTPPASNSCTPSSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPIDSQMGASTNSSNHPMSSKHNMSGGEFQSKREKSDKDKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGDSGGEGLRPQIASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNVDSESESGSSIAERSYQNSPSSDDGVRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPENWSKSPISSDQTSSMTNNPILSADRPSRLSPDFMIGEEDDDLMDVALIGN

Target ORF information:

RefSeq Version	XM_017597471.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mediator complex subunit 1 (Med1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597471.1

ORF Insert Sequence:

ATGTATCTGG CTCTCCAGTC CTTAGAACAG GACCTTTCTA AAATGGCAAT TATGTACTGG 
AAGGCAACTA ATGCTACTCC CTTGGATAAG ATTCTTCACG GAAGTGTTGG CTATCTCACT 
CCACGGAGCG GGGGTCATTT AATGAACATG AAATACTATG CCTCTCCGTC TGACCTGCTG 
CATGATACAA CTGCCTCTCC TATCACTTTG CATGAAAAGA ATGTTCCTCG GTCTTTGGGA 
ATGAATGCCT CAGTGACAAT TGAAGGAACC TCTGCTATGT ACAAACTCCC AATTGCCCCA 
TTAATTATGG GGTCACACCC AGCTGACAAC AAATGGACCC CTTCTTTCTC TGCAGTCACT 
AGTGCCAACA GTGTTGATCT TCCTGCGTGT TTCTTCTTGA AATTTCCCCA GCCAATCCCA 
GTATCTAAAG CATTTGTTCA GAAACTGCAA AATTGCACGG GAATCCCGTT GTTTGAGACT 
CCGCCTACTT ACCTGCCCCT CTATGAACTC ATCACTCAGT TTGAGCTGTC AAAGGATCCT 
GACCCTTTAC CTTTGAATCA CAACATGCGA TTTTACGCTG CTCTTCCAGG TCAGCAGCAC 
TGCTATTTTC TCAATAAAGA TGCTCCTCTT CCTGATGGCC AAAGCCTGCA GGGAACACTG 
GTCAGCAAAA TCACCTTCCA GCACCCTGGC CGAGTTCCTC TTATCTTGAA TATGATCAGA 
CACCAAGTGG CCTATAACAC CCTAATTGGA AGCTGTGTTA AAAGAACTAT TTTAAAAGAA 
GATTCTCCTG GGCTCCTCCA ATTTGAAGTG TGTCCTCTTT CAGAATCTCG ATTCAGTGTA 
TCTTTTCAGC ACCCTGTGAA TGACTCCCTT GTGTGTGTGG TGATGGACGT GCAAGACTCA 
ACACATGTGA GCTGTAAACT CTACAAGGGG CTATCAGATG CACTGATCTG TACAGACGAC 
TTCATTGCCA AAGTTGTTCA AAGATGTATG TCCATCCCTG TGACGATGAG GGCTATTCGG 
AGGAAGGCTG AAACCATACA GGCTGACACG CCAGCACTGT CCCTCATTGC AGAGACAGTC 
GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA 
GGCAGCAACC CAATGAGCGG TACCACCACA CCAACCAACA CCTTTCCGGG GGGTCCCATT 
ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG 
GAGGACTTCA GCAAGGTGTC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG 
AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC 
TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAC 
CCTGCCCAAG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCCTCT 
TCCGGATCAC CCCGGATGGA AATGTGCTCG GGGAGCAACA AGACCAAGAA AAATAAGTCA 
TCAAGAGTAC CACCGGACAA ACCCAAGCAC CAGACCGAAG ATGATTTTCA GAGGGAGCTC 
TTTTCAATGG ATGTCGACTC ACAGAACCCT ATGTTCGATG TCAGCATGAC AGCTGACGCG 
CTGGATACAC CTCATATCAC TCCAGCTCCA AGCCAGTGTA GTACGCCCCC AACAACGTAC 
CCACAGCCAG TGTCTCACCC CCAGCCCAGT ATTCAAAGGA TGGGCCGACT GTCCAGTTCA 
GACAGCATTG GCCCAGATGT AACTGATATT CTTTCAGACA TTGCAGAAGA AGCTTCAAAG 
CTTCCCAGCA CTAGTGATGA TTGCCCACCC ATTGGTACCC CAGTTCGAGA TTCCTCGAGT 
TCTGGGCATT CTCAGAGTGC CCTCTTTGAC TCTGATGTCT TTCAGACTAA TAACAATGAA 
AATCCATACA CTGACCCGGC TGACCTTATT GCAGATGCTG CTGGGAGCCC CAGTAGTGAT 
TCTCCTACCA ATCATTTTTT CCCTGATGGA GTAGATTTCA ATCCTGATTT GTTGAACAGC 
CAAAGCCAAA GTGGTTTTGG AGAGGAGTAT TTTGATGAAA GTAGTCAGAG TGGGGATAAT 
GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGATGCC AATGCTCGGA 
GGTGACAATG GGGAGTCGAA ATTGAAGGGC AGCAGCCAAG CTGACACAGT TGACTTCAGT 
ATTCTGTCAG TAGCTGGTAA GGCTTTGGGT GCAGCAGACC TTCTGGAGCA CCACAGTGGT 
AGTCAGAGTC CTCTGCTGAC CACTGGAGAA TTAGGGAAAG AAAAAACTCA AAAGAGGGTG 
AAGGAAGGCA ACGGTATGGG CGCTAGCAGT GGGTCAGGCC CGGGGTCAGA CAGCAAGCCA 
GGCAAGCGCA GCCGCACACC TTCCAATGAT GGGAAGAGCA AAGATAAGCC TCCAAAGCGA 
AAGAAGGCAG ACACGGAGGG AAAATCCCCA TCTCACAGTT CTAATAGACC TTTCACCCCA 
CCCACCAGTA CAGGTGGGTC CAAATCCCCT GGCAGTTCAG GACGATGTCA GACGCCCCCT 
GGTGTCGCCA CACCGCCCAT TCCCAAGATC ACCATTCAGA TTCCTAAAGG AACAGTGATG 
GTGGGCAAGC CTTCCTCGCA CAGTCAGTAC TCTAGCAGTG GTTCTGTGTC TTCCTCTGGC 
AGCAAAAGCC ACCACAGTCA TTCTTCCTCC TCCTCTTCCT TATCTTCTGC TTCCACCTCA 
GGCAAGATGA AAAGCAGTAA ATCAGAAGGC TCCTCAAGTG CCAAGCTCAG TGGCAGTATG 
TATTCTAGCC AAGGGTCTTC TGGATCCAGC CAGTCCAAAA ATTCATCTCA GACTGGGGGG 
AAGCCAGGCT CCTCTCCCAT TACCAAACAT GGACTGAGTA GTGGGTCTAG CAGCACCAAG 
ATGAAACCTC AAGGCAAGCC ATCCTCACTT ATGAATCCTT CTATAAGTAA ACCAAACATA 
TCCCCTTCCC ATTCAAGGCC TCCTGGAGGC TCAGATAAGC TTGCCTCTCC AATGAAGCCT 
GTTCCCGGGA CCCCCCCATC CTCTAAAGCC AAGTCCCCTA TCAGTTCAGG TTCTGGTAGC 
TCTCATGTGT CAGGAACTAG TTCAAGCTCT GGTATGAAGT CTTCTTCAGG GTCAGGATCC 
TCAGGCTCAT TGTCTCAAAA AACTCCCCCA GCATCTAATT CTTGTACACC ATCTTCCTCC 
TCATTTTCCT CAAGTGGTTC TTCCATGTCA TCCTCTCAGA ATCAACATGG GAGTTCCAAA 
GGGAAATCTC CCAGTAGGAA TAAGAAGCCT TCTTTGACAG CTGTCATAGA TAAATTGAAG 
CATGGGGTGG TTACCAGTGG GCCTGGGGGT GAGGATCCAA TAGACAGTCA GATGGGTGCA 
AGCACAAATT CCTCTAACCA TCCCATGTCC TCCAAACATA ACATGTCAGG AGGAGAATTC 
CAGAGCAAAC GTGAGAAAAG CGATAAAGAC AAATCAAAGG TTTCTACTTC TGGAGGATCA 
GTTGATTCCT CTAAGAAGAC TTCAGAGTCA AAAAATGTGG GGAGCACGGG TGTGGCAAAA 
ATTATTATCA GCAAGCACGA TGGAGGCTCC CCGAGCATCA AAGCCAAAGT GACTCTACAG 
AAACCTGGAG ATAGTGGTGG AGAAGGGCTC AGGCCACAGA TCGCCTCTTC AAAAAACTAT 
GGCTCTCCAT TGATCAGTGG TTCTACTCCA AAGCATGAAC GGGGTTCTCC CAGCCACAGT 
AAGTCGCCAG CATATACACC TCAGAATGTG GACAGTGAAA GTGAGTCAGG CTCCTCCATA 
GCAGAGAGAT CCTACCAGAA CAGCCCCAGC TCAGATGATG GTGTCCGGCC ACTCCCAGAA 
TACAGCACAG AGAAGCATAA GAAGCACAAA AAGGAAAAGA AAAAAGTAAA AGATAAAGAT 
AGAGACCGGG ACAGAGACAA AGACAGAGAC AAGAAAAAAT CTCACAGCAT CAAGCCAGAG 
AATTGGTCTA AATCCCCCAT TTCTTCAGAT CAGACTTCAT CTATGACAAA TAACCCTATC 
TTATCTGCAG ACAGGCCTTC CAGGCTCAGC CCTGACTTTA TGATTGGGGA GGAAGATGAT 
GATCTTATGG ATGTGGCTCT GATTGGCAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med1 ( NM_001134361.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001127833.1 Rattus norvegicus mediator complex subunit 1 (Med1), mRNA.

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CloneID	ORa02628
Clone ID Related Accession (Same CDS sequence)	NM_001134361.1 , XM_006247399.3
Accession Version	NM_001134361.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	mediator of RNA polymerase II transcription subunit 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473948.1. On or before Aug 26, 2008 this sequence version replaced XM_001081351.1, XM_573188.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAGTTCTC TCTTGGACCG GCTCCATGCA AAATTTAACC AAAGTAGACC TTGGAGTGAG 
ACCATTAAGC TTGTGCGTCA AGTCATGGAG AAGAGGGTCG TAATGAGTTC TGGAGGACAT 
CAGCATTTGG TCAGCTGTTT GGAGACATTA CAGAAGGCTC TCAAAGTAAC ATCTTTGCCA 
GCAATGACTG ATCGTTTGGA ATCCATAGCC AGACAGAATG GACTGGGCTC TCATCTCAGT 
GCCAGTGGCA CTGAATGTTA CATCACGTCA GATATGTTCT ATGTGGAAGT GCAGTTAGAC 
CCTGCAGGAC AGCTTTGTGA TGTAAAAGTG GCTCACCATG GGGAGAATCC TGTGAGCTGT 
CCAGAGCTTG TGCAGCAGTT AAGGGAAAAG AATTTTGATG AGTTTTCTAA GCATCTTAAG 
GGTCTTGTTA ATCTGTATAA CCTTCCAGGA GACAACAAAC TGAAGACTAA GATGTATCTG 
GCTCTCCAGT CCTTAGAACA GGACCTTTCT AAAATGGCAA TTATGTACTG GAAGGCAACT 
AATGCTACTC CCTTGGATAA GATTCTTCAC GGAAGTGTTG GCTATCTCAC TCCACGGAGC 
GGGGGTCATT TAATGAACAT GAAATACTAT GCCTCTCCGT CTGACCTGCT GCATGATACA 
ACTGCCTCTC CTATCACTTT GCATGAAAAG AATGTTCCTC GGTCTTTGGG AATGAATGCC 
TCAGTGACAA TTGAAGGAAC CTCTGCTATG TACAAACTCC CAATTGCCCC ATTAATTATG 
GGGTCACACC CAGCTGACAA CAAATGGACC CCTTCTTTCT CTGCAGTCAC TAGTGCCAAC 
AGTGTTGATC TTCCTGCGTG TTTCTTCTTG AAATTTCCCC AGCCAATCCC AGTATCTAAA 
GCATTTGTTC AGAAACTGCA AAATTGCACG GGAATCCCGT TGTTTGAGAC TCCGCCTACT 
TACCTGCCCC TCTATGAACT CATCACTCAG TTTGAGCTGT CAAAGGATCC TGACCCTTTA 
CCTTTGAATC ACAACATGCG ATTTTACGCT GCTCTTCCAG GTCAGCAGCA CTGCTATTTT 
CTCAATAAAG ATGCTCCTCT TCCTGATGGC CAAAGCCTGC AGGGAACACT GGTCAGCAAA 
ATCACCTTCC AGCACCCTGG CCGAGTTCCT CTTATCTTGA ATATGATCAG ACACCAAGTG 
GCCTATAACA CCCTAATTGG AAGCTGTGTT AAAAGAACTA TTTTAAAAGA AGATTCTCCT 
GGGCTCCTCC AATTTGAAGT GTGTCCTCTT TCAGAATCTC GATTCAGTGT ATCTTTTCAG 
CACCCTGTGA ATGACTCCCT TGTGTGTGTG GTGATGGACG TGCAAGACTC AACACATGTG 
AGCTGTAAAC TCTACAAGGG GCTATCAGAT GCACTGATCT GTACAGACGA CTTCATTGCC 
AAAGTTGTTC AAAGATGTAT GTCCATCCCT GTGACGATGA GGGCTATTCG GAGGAAGGCT 
GAAACCATAC AGGCTGACAC GCCAGCACTG TCCCTCATTG CAGAGACAGT CGAAGACATG 
GTGAAAAAGA ACCTGCCCCC GGCTAGCAGC CCAGGGTATG GCATGACCAC AGGCAGCAAC 
CCAATGAGCG GTACCACCAC ACCAACCAAC ACCTTTCCGG GGGGTCCCAT TACCACCTTG 
TTTAATATGA GCATGAGCAT CAAAGATCGG CATGAGTCGG TGGGCCATGG GGAGGACTTC 
AGCAAGGTGT CTCAGAACCC AATTCTTACC AGTTTGTTGC AAATCACAGG GAACGGGGGG 
TCTACCATTG GCTCGAGTCC GACCCCTCCT CATCACACGC CGCCACCTGT CTCTTCGATG 
GCCGGCAACA CCAAGAACCA CCCGATGCTC ATGAACCTTC TTAAAGATAA CCCTGCCCAA 
GATTTCTCAA CCCTTTATGG AAGCAGCCCT TTAGAAAGGC AGAACTCCTC TTCCGGATCA 
CCCCGGATGG AAATGTGCTC GGGGAGCAAC AAGACCAAGA AAAATAAGTC ATCAAGAGTA 
CCACCGGACA AACCCAAGCA CCAGACCGAA GATGATTTTC AGAGGGAGCT CTTTTCAATG 
GATGTCGACT CACAGAACCC TATGTTCGAT GTCAGCATGA CAGCTGACGC GCTGGATACA 
CCTCATATCA CTCCAGCTCC AAGCCAGTGT AGTACGCCCC CAACAACGTA CCCACAGCCA 
GTGTCTCACC CCCAGCCCAG TATTCAAAGG ATGGGCCGAC TGTCCAGTTC AGACAGCATT 
GGCCCAGATG TAACTGATAT TCTTTCAGAC ATTGCAGAAG AAGCTTCAAA GCTTCCCAGC 
ACTAGTGATG ATTGCCCACC CATTGGTACC CCAGTTCGAG ATTCCTCGAG TTCTGGGCAT 
TCTCAGAGTG CCCTCTTTGA CTCTGATGTC TTTCAGACTA ATAACAATGA AAATCCATAC 
ACTGACCCGG CTGACCTTAT TGCAGATGCT GCTGGGAGCC CCAGTAGTGA TTCTCCTACC 
AATCATTTTT TCCCTGATGG AGTAGATTTC AATCCTGATT TGTTGAACAG CCAAAGCCAA 
AGTGGTTTTG GAGAGGAGTA TTTTGATGAA AGTAGTCAGA GTGGGGATAA TGATGATTTC 
AAAGGATTTG CATCTCAGGC ACTAAATACT TTGGGGATGC CAATGCTCGG AGGTGACAAT 
GGGGAGTCGA AATTGAAGGG CAGCAGCCAA GCTGACACAG TTGACTTCAG TATTCTGTCA 
GTAGCTGGTA AGGCTTTGGG TGCAGCAGAC CTTCTGGAGC ACCACAGTGG TAGTCAGAGT 
CCTCTGCTGA CCACTGGAGA ATTAGGGAAA GAAAAAACTC AAAAGAGGGT GAAGGAAGGC 
AACGGTATGG GCGCTAGCAG TGGGTCAGGC CCGGGGTCAG ACAGCAAGCC AGGCAAGCGC 
AGCCGCACAC CTTCCAATGA TGGGAAGAGC AAAGATAAGC CTCCAAAGCG AAAGAAGGCA 
GACACGGAGG GAAAATCCCC ATCTCACAGT TCTAATAGAC CTTTCACCCC ACCCACCAGT 
ACAGGTGGGT CCAAATCCCC TGGCAGTTCA GGACGATGTC AGACGCCCCC TGGTGTCGCC 
ACACCGCCCA TTCCCAAGAT CACCATTCAG ATTCCTAAAG GAACAGTGAT GGTGGGCAAG 
CCTTCCTCGC ACAGTCAGTA CTCTAGCAGT GGTTCTGTGT CTTCCTCTGG CAGCAAAAGC 
CACCACAGTC ATTCTTCCTC CTCCTCTTCC TTATCTTCTG CTTCCACCTC AGGCAAGATG 
AAAAGCAGTA AATCAGAAGG CTCCTCAAGT GCCAAGCTCA GTGGCAGTAT GTATTCTAGC 
CAAGGGTCTT CTGGATCCAG CCAGTCCAAA AATTCATCTC AGACTGGGGG GAAGCCAGGC 
TCCTCTCCCA TTACCAAACA TGGACTGAGT AGTGGGTCTA GCAGCACCAA GATGAAACCT 
CAAGGCAAGC CATCCTCACT TATGAATCCT TCTATAAGTA AACCAAACAT ATCCCCTTCC 
CATTCAAGGC CTCCTGGAGG CTCAGATAAG CTTGCCTCTC CAATGAAGCC TGTTCCCGGG 
ACCCCCCCAT CCTCTAAAGC CAAGTCCCCT ATCAGTTCAG GTTCTGGTAG CTCTCATGTG 
TCAGGAACTA GTTCAAGCTC TGGTATGAAG TCTTCTTCAG GGTCAGGATC CTCAGGCTCA 
TTGTCTCAAA AAACTCCCCC AGCATCTAAT TCTTGTACAC CATCTTCCTC CTCATTTTCC 
TCAAGTGGTT CTTCCATGTC ATCCTCTCAG AATCAACATG GGAGTTCCAA AGGGAAATCT 
CCCAGTAGGA ATAAGAAGCC TTCTTTGACA GCTGTCATAG ATAAATTGAA GCATGGGGTG 
GTTACCAGTG GGCCTGGGGG TGAGGATCCA ATAGACAGTC AGATGGGTGC AAGCACAAAT 
TCCTCTAACC ATCCCATGTC CTCCAAACAT AACATGTCAG GAGGAGAATT CCAGAGCAAA 
CGTGAGAAAA GCGATAAAGA CAAATCAAAG GTTTCTACTT CTGGAGGATC AGTTGATTCC 
TCTAAGAAGA CTTCAGAGTC AAAAAATGTG GGGAGCACGG GTGTGGCAAA AATTATTATC 
AGCAAGCACG ATGGAGGCTC CCCGAGCATC AAAGCCAAAG TGACTCTACA GAAACCTGGA 
GATAGTGGTG GAGAAGGGCT CAGGCCACAG ATCGCCTCTT CAAAAAACTA TGGCTCTCCA 
TTGATCAGTG GTTCTACTCC AAAGCATGAA CGGGGTTCTC CCAGCCACAG TAAGTCGCCA 
GCATATACAC CTCAGAATGT GGACAGTGAA AGTGAGTCAG GCTCCTCCAT AGCAGAGAGA 
TCCTACCAGA ACAGCCCCAG CTCAGATGAT GGTGTCCGGC CACTCCCAGA ATACAGCACA 
GAGAAGCATA AGAAGCACAA AAAGGAAAAG AAAAAAGTAA AAGATAAAGA TAGAGACCGG 
GACAGAGACA AAGACAGAGA CAAGAAAAAA TCTCACAGCA TCAAGCCAGA GAATTGGTCT 
AAATCCCCCA TTTCTTCAGA TCAGACTTCA TCTATGACAA ATAACCCTAT CTTATCTGCA 
GACAGGCCTT CCAGGCTCAG CCCTGACTTT ATGATTGGGG AGGAAGATGA TGATCTTATG 
GATGTGGCTC TGATTGGCAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001127833.1
CDS	118..4821
Translation	MSSLLDRLHAKFNQSRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNATPLDKILHGSVGYLTPRSGGHLMNMKYYASPSDLLHDTTASPITLHEKNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPADNKWTPSFSAVTSANSVDLPACFFLKFPQPIPVSKAFVQKLQNCTGIPLFETPPTYLPLYELITQFELSKDPDPLPLNHNMRFYAALPGQQHCYFLNKDAPLPDGQSLQGTLVSKITFQHPGRVPLILNMIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGSNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMCSGSNKTKKNKSSRVPPDKPKHQTEDDFQRELFSMDVDSQNPMFDVSMTADALDTPHITPAPSQCSTPPTTYPQPVSHPQPSIQRMGRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPPIGTPVRDSSSSGHSQSALFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGMPMLGGDNGESKLKGSSQADTVDFSILSVAGKALGAADLLEHHSGSQSPLLTTGELGKEKTQKRVKEGNGMGASSGSGPGSDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSNRPFTPPTSTGGSKSPGSSGRCQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYSSSGSVSSSGSKSHHSHSSSSSSLSSASTSGKMKSSKSEGSSSAKLSGSMYSSQGSSGSSQSKNSSQTGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSISKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGSSHVSGTSSSSGMKSSSGSGSSGSLSQKTPPASNSCTPSSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPIDSQMGASTNSSNHPMSSKHNMSGGEFQSKREKSDKDKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGDSGGEGLRPQIASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNVDSESESGSSIAERSYQNSPSSDDGVRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPENWSKSPISSDQTSSMTNNPILSADRPSRLSPDFMIGEEDDDLMDVALIGN

Target ORF information:

RefSeq Version	NM_001134361.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mediator complex subunit 1 (Med1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134361.1

ORF Insert Sequence:

ATGAGTTCTC TCTTGGACCG GCTCCATGCA AAATTTAACC AAAGTAGACC TTGGAGTGAG 
ACCATTAAGC TTGTGCGTCA AGTCATGGAG AAGAGGGTCG TAATGAGTTC TGGAGGACAT 
CAGCATTTGG TCAGCTGTTT GGAGACATTA CAGAAGGCTC TCAAAGTAAC ATCTTTGCCA 
GCAATGACTG ATCGTTTGGA ATCCATAGCC AGACAGAATG GACTGGGCTC TCATCTCAGT 
GCCAGTGGCA CTGAATGTTA CATCACGTCA GATATGTTCT ATGTGGAAGT GCAGTTAGAC 
CCTGCAGGAC AGCTTTGTGA TGTAAAAGTG GCTCACCATG GGGAGAATCC TGTGAGCTGT 
CCAGAGCTTG TGCAGCAGTT AAGGGAAAAG AATTTTGATG AGTTTTCTAA GCATCTTAAG 
GGTCTTGTTA ATCTGTATAA CCTTCCAGGA GACAACAAAC TGAAGACTAA GATGTATCTG 
GCTCTCCAGT CCTTAGAACA GGACCTTTCT AAAATGGCAA TTATGTACTG GAAGGCAACT 
AATGCTACTC CCTTGGATAA GATTCTTCAC GGAAGTGTTG GCTATCTCAC TCCACGGAGC 
GGGGGTCATT TAATGAACAT GAAATACTAT GCCTCTCCGT CTGACCTGCT GCATGATACA 
ACTGCCTCTC CTATCACTTT GCATGAAAAG AATGTTCCTC GGTCTTTGGG AATGAATGCC 
TCAGTGACAA TTGAAGGAAC CTCTGCTATG TACAAACTCC CAATTGCCCC ATTAATTATG 
GGGTCACACC CAGCTGACAA CAAATGGACC CCTTCTTTCT CTGCAGTCAC TAGTGCCAAC 
AGTGTTGATC TTCCTGCGTG TTTCTTCTTG AAATTTCCCC AGCCAATCCC AGTATCTAAA 
GCATTTGTTC AGAAACTGCA AAATTGCACG GGAATCCCGT TGTTTGAGAC TCCGCCTACT 
TACCTGCCCC TCTATGAACT CATCACTCAG TTTGAGCTGT CAAAGGATCC TGACCCTTTA 
CCTTTGAATC ACAACATGCG ATTTTACGCT GCTCTTCCAG GTCAGCAGCA CTGCTATTTT 
CTCAATAAAG ATGCTCCTCT TCCTGATGGC CAAAGCCTGC AGGGAACACT GGTCAGCAAA 
ATCACCTTCC AGCACCCTGG CCGAGTTCCT CTTATCTTGA ATATGATCAG ACACCAAGTG 
GCCTATAACA CCCTAATTGG AAGCTGTGTT AAAAGAACTA TTTTAAAAGA AGATTCTCCT 
GGGCTCCTCC AATTTGAAGT GTGTCCTCTT TCAGAATCTC GATTCAGTGT ATCTTTTCAG 
CACCCTGTGA ATGACTCCCT TGTGTGTGTG GTGATGGACG TGCAAGACTC AACACATGTG 
AGCTGTAAAC TCTACAAGGG GCTATCAGAT GCACTGATCT GTACAGACGA CTTCATTGCC 
AAAGTTGTTC AAAGATGTAT GTCCATCCCT GTGACGATGA GGGCTATTCG GAGGAAGGCT 
GAAACCATAC AGGCTGACAC GCCAGCACTG TCCCTCATTG CAGAGACAGT CGAAGACATG 
GTGAAAAAGA ACCTGCCCCC GGCTAGCAGC CCAGGGTATG GCATGACCAC AGGCAGCAAC 
CCAATGAGCG GTACCACCAC ACCAACCAAC ACCTTTCCGG GGGGTCCCAT TACCACCTTG 
TTTAATATGA GCATGAGCAT CAAAGATCGG CATGAGTCGG TGGGCCATGG GGAGGACTTC 
AGCAAGGTGT CTCAGAACCC AATTCTTACC AGTTTGTTGC AAATCACAGG GAACGGGGGG 
TCTACCATTG GCTCGAGTCC GACCCCTCCT CATCACACGC CGCCACCTGT CTCTTCGATG 
GCCGGCAACA CCAAGAACCA CCCGATGCTC ATGAACCTTC TTAAAGATAA CCCTGCCCAA 
GATTTCTCAA CCCTTTATGG AAGCAGCCCT TTAGAAAGGC AGAACTCCTC TTCCGGATCA 
CCCCGGATGG AAATGTGCTC GGGGAGCAAC AAGACCAAGA AAAATAAGTC ATCAAGAGTA 
CCACCGGACA AACCCAAGCA CCAGACCGAA GATGATTTTC AGAGGGAGCT CTTTTCAATG 
GATGTCGACT CACAGAACCC TATGTTCGAT GTCAGCATGA CAGCTGACGC GCTGGATACA 
CCTCATATCA CTCCAGCTCC AAGCCAGTGT AGTACGCCCC CAACAACGTA CCCACAGCCA 
GTGTCTCACC CCCAGCCCAG TATTCAAAGG ATGGGCCGAC TGTCCAGTTC AGACAGCATT 
GGCCCAGATG TAACTGATAT TCTTTCAGAC ATTGCAGAAG AAGCTTCAAA GCTTCCCAGC 
ACTAGTGATG ATTGCCCACC CATTGGTACC CCAGTTCGAG ATTCCTCGAG TTCTGGGCAT 
TCTCAGAGTG CCCTCTTTGA CTCTGATGTC TTTCAGACTA ATAACAATGA AAATCCATAC 
ACTGACCCGG CTGACCTTAT TGCAGATGCT GCTGGGAGCC CCAGTAGTGA TTCTCCTACC 
AATCATTTTT TCCCTGATGG AGTAGATTTC AATCCTGATT TGTTGAACAG CCAAAGCCAA 
AGTGGTTTTG GAGAGGAGTA TTTTGATGAA AGTAGTCAGA GTGGGGATAA TGATGATTTC 
AAAGGATTTG CATCTCAGGC ACTAAATACT TTGGGGATGC CAATGCTCGG AGGTGACAAT 
GGGGAGTCGA AATTGAAGGG CAGCAGCCAA GCTGACACAG TTGACTTCAG TATTCTGTCA 
GTAGCTGGTA AGGCTTTGGG TGCAGCAGAC CTTCTGGAGC ACCACAGTGG TAGTCAGAGT 
CCTCTGCTGA CCACTGGAGA ATTAGGGAAA GAAAAAACTC AAAAGAGGGT GAAGGAAGGC 
AACGGTATGG GCGCTAGCAG TGGGTCAGGC CCGGGGTCAG ACAGCAAGCC AGGCAAGCGC 
AGCCGCACAC CTTCCAATGA TGGGAAGAGC AAAGATAAGC CTCCAAAGCG AAAGAAGGCA 
GACACGGAGG GAAAATCCCC ATCTCACAGT TCTAATAGAC CTTTCACCCC ACCCACCAGT 
ACAGGTGGGT CCAAATCCCC TGGCAGTTCA GGACGATGTC AGACGCCCCC TGGTGTCGCC 
ACACCGCCCA TTCCCAAGAT CACCATTCAG ATTCCTAAAG GAACAGTGAT GGTGGGCAAG 
CCTTCCTCGC ACAGTCAGTA CTCTAGCAGT GGTTCTGTGT CTTCCTCTGG CAGCAAAAGC 
CACCACAGTC ATTCTTCCTC CTCCTCTTCC TTATCTTCTG CTTCCACCTC AGGCAAGATG 
AAAAGCAGTA AATCAGAAGG CTCCTCAAGT GCCAAGCTCA GTGGCAGTAT GTATTCTAGC 
CAAGGGTCTT CTGGATCCAG CCAGTCCAAA AATTCATCTC AGACTGGGGG GAAGCCAGGC 
TCCTCTCCCA TTACCAAACA TGGACTGAGT AGTGGGTCTA GCAGCACCAA GATGAAACCT 
CAAGGCAAGC CATCCTCACT TATGAATCCT TCTATAAGTA AACCAAACAT ATCCCCTTCC 
CATTCAAGGC CTCCTGGAGG CTCAGATAAG CTTGCCTCTC CAATGAAGCC TGTTCCCGGG 
ACCCCCCCAT CCTCTAAAGC CAAGTCCCCT ATCAGTTCAG GTTCTGGTAG CTCTCATGTG 
TCAGGAACTA GTTCAAGCTC TGGTATGAAG TCTTCTTCAG GGTCAGGATC CTCAGGCTCA 
TTGTCTCAAA AAACTCCCCC AGCATCTAAT TCTTGTACAC CATCTTCCTC CTCATTTTCC 
TCAAGTGGTT CTTCCATGTC ATCCTCTCAG AATCAACATG GGAGTTCCAA AGGGAAATCT 
CCCAGTAGGA ATAAGAAGCC TTCTTTGACA GCTGTCATAG ATAAATTGAA GCATGGGGTG 
GTTACCAGTG GGCCTGGGGG TGAGGATCCA ATAGACAGTC AGATGGGTGC AAGCACAAAT 
TCCTCTAACC ATCCCATGTC CTCCAAACAT AACATGTCAG GAGGAGAATT CCAGAGCAAA 
CGTGAGAAAA GCGATAAAGA CAAATCAAAG GTTTCTACTT CTGGAGGATC AGTTGATTCC 
TCTAAGAAGA CTTCAGAGTC AAAAAATGTG GGGAGCACGG GTGTGGCAAA AATTATTATC 
AGCAAGCACG ATGGAGGCTC CCCGAGCATC AAAGCCAAAG TGACTCTACA GAAACCTGGA 
GATAGTGGTG GAGAAGGGCT CAGGCCACAG ATCGCCTCTT CAAAAAACTA TGGCTCTCCA 
TTGATCAGTG GTTCTACTCC AAAGCATGAA CGGGGTTCTC CCAGCCACAG TAAGTCGCCA 
GCATATACAC CTCAGAATGT GGACAGTGAA AGTGAGTCAG GCTCCTCCAT AGCAGAGAGA 
TCCTACCAGA ACAGCCCCAG CTCAGATGAT GGTGTCCGGC CACTCCCAGA ATACAGCACA 
GAGAAGCATA AGAAGCACAA AAAGGAAAAG AAAAAAGTAA AAGATAAAGA TAGAGACCGG 
GACAGAGACA AAGACAGAGA CAAGAAAAAA TCTCACAGCA TCAAGCCAGA GAATTGGTCT 
AAATCCCCCA TTTCTTCAGA TCAGACTTCA TCTATGACAA ATAACCCTAT CTTATCTGCA 
GACAGGCCTT CCAGGCTCAG CCCTGACTTT ATGATTGGGG AGGAAGATGA TGATCTTATG 
GATGTGGCTC TGATTGGCAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med1 ( XM_006247399.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247461.1 Rattus norvegicus mediator complex subunit 1 (Med1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa02628
Clone ID Related Accession (Same CDS sequence)	NM_001134361.1 , XM_006247399.3
Accession Version	XM_006247399.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mediator of RNA polymerase II transcription subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247399.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCTC TCTTGGACCG GCTCCATGCA AAATTTAACC AAAGTAGACC TTGGAGTGAG 
ACCATTAAGC TTGTGCGTCA AGTCATGGAG AAGAGGGTCG TAATGAGTTC TGGAGGACAT 
CAGCATTTGG TCAGCTGTTT GGAGACATTA CAGAAGGCTC TCAAAGTAAC ATCTTTGCCA 
GCAATGACTG ATCGTTTGGA ATCCATAGCC AGACAGAATG GACTGGGCTC TCATCTCAGT 
GCCAGTGGCA CTGAATGTTA CATCACGTCA GATATGTTCT ATGTGGAAGT GCAGTTAGAC 
CCTGCAGGAC AGCTTTGTGA TGTAAAAGTG GCTCACCATG GGGAGAATCC TGTGAGCTGT 
CCAGAGCTTG TGCAGCAGTT AAGGGAAAAG AATTTTGATG AGTTTTCTAA GCATCTTAAG 
GGTCTTGTTA ATCTGTATAA CCTTCCAGGA GACAACAAAC TGAAGACTAA GATGTATCTG 
GCTCTCCAGT CCTTAGAACA GGACCTTTCT AAAATGGCAA TTATGTACTG GAAGGCAACT 
AATGCTACTC CCTTGGATAA GATTCTTCAC GGAAGTGTTG GCTATCTCAC TCCACGGAGC 
GGGGGTCATT TAATGAACAT GAAATACTAT GCCTCTCCGT CTGACCTGCT GCATGATACA 
ACTGCCTCTC CTATCACTTT GCATGAAAAG AATGTTCCTC GGTCTTTGGG AATGAATGCC 
TCAGTGACAA TTGAAGGAAC CTCTGCTATG TACAAACTCC CAATTGCCCC ATTAATTATG 
GGGTCACACC CAGCTGACAA CAAATGGACC CCTTCTTTCT CTGCAGTCAC TAGTGCCAAC 
AGTGTTGATC TTCCTGCGTG TTTCTTCTTG AAATTTCCCC AGCCAATCCC AGTATCTAAA 
GCATTTGTTC AGAAACTGCA AAATTGCACG GGAATCCCGT TGTTTGAGAC TCCGCCTACT 
TACCTGCCCC TCTATGAACT CATCACTCAG TTTGAGCTGT CAAAGGATCC TGACCCTTTA 
CCTTTGAATC ACAACATGCG ATTTTACGCT GCTCTTCCAG GTCAGCAGCA CTGCTATTTT 
CTCAATAAAG ATGCTCCTCT TCCTGATGGC CAAAGCCTGC AGGGAACACT GGTCAGCAAA 
ATCACCTTCC AGCACCCTGG CCGAGTTCCT CTTATCTTGA ATATGATCAG ACACCAAGTG 
GCCTATAACA CCCTAATTGG AAGCTGTGTT AAAAGAACTA TTTTAAAAGA AGATTCTCCT 
GGGCTCCTCC AATTTGAAGT GTGTCCTCTT TCAGAATCTC GATTCAGTGT ATCTTTTCAG 
CACCCTGTGA ATGACTCCCT TGTGTGTGTG GTGATGGACG TGCAAGACTC AACACATGTG 
AGCTGTAAAC TCTACAAGGG GCTATCAGAT GCACTGATCT GTACAGACGA CTTCATTGCC 
AAAGTTGTTC AAAGATGTAT GTCCATCCCT GTGACGATGA GGGCTATTCG GAGGAAGGCT 
GAAACCATAC AGGCTGACAC GCCAGCACTG TCCCTCATTG CAGAGACAGT CGAAGACATG 
GTGAAAAAGA ACCTGCCCCC GGCTAGCAGC CCAGGGTATG GCATGACCAC AGGCAGCAAC 
CCAATGAGCG GTACCACCAC ACCAACCAAC ACCTTTCCGG GGGGTCCCAT TACCACCTTG 
TTTAATATGA GCATGAGCAT CAAAGATCGG CATGAGTCGG TGGGCCATGG GGAGGACTTC 
AGCAAGGTGT CTCAGAACCC AATTCTTACC AGTTTGTTGC AAATCACAGG GAACGGGGGG 
TCTACCATTG GCTCGAGTCC GACCCCTCCT CATCACACGC CGCCACCTGT CTCTTCGATG 
GCCGGCAACA CCAAGAACCA CCCGATGCTC ATGAACCTTC TTAAAGATAA CCCTGCCCAA 
GATTTCTCAA CCCTTTATGG AAGCAGCCCT TTAGAAAGGC AGAACTCCTC TTCCGGATCA 
CCCCGGATGG AAATGTGCTC GGGGAGCAAC AAGACCAAGA AAAATAAGTC ATCAAGAGTA 
CCACCGGACA AACCCAAGCA CCAGACCGAA GATGATTTTC AGAGGGAGCT CTTTTCAATG 
GATGTCGACT CACAGAACCC TATGTTCGAT GTCAGCATGA CAGCTGACGC GCTGGATACA 
CCTCATATCA CTCCAGCTCC AAGCCAGTGT AGTACGCCCC CAACAACGTA CCCACAGCCA 
GTGTCTCACC CCCAGCCCAG TATTCAAAGG ATGGGCCGAC TGTCCAGTTC AGACAGCATT 
GGCCCAGATG TAACTGATAT TCTTTCAGAC ATTGCAGAAG AAGCTTCAAA GCTTCCCAGC 
ACTAGTGATG ATTGCCCACC CATTGGTACC CCAGTTCGAG ATTCCTCGAG TTCTGGGCAT 
TCTCAGAGTG CCCTCTTTGA CTCTGATGTC TTTCAGACTA ATAACAATGA AAATCCATAC 
ACTGACCCGG CTGACCTTAT TGCAGATGCT GCTGGGAGCC CCAGTAGTGA TTCTCCTACC 
AATCATTTTT TCCCTGATGG AGTAGATTTC AATCCTGATT TGTTGAACAG CCAAAGCCAA 
AGTGGTTTTG GAGAGGAGTA TTTTGATGAA AGTAGTCAGA GTGGGGATAA TGATGATTTC 
AAAGGATTTG CATCTCAGGC ACTAAATACT TTGGGGATGC CAATGCTCGG AGGTGACAAT 
GGGGAGTCGA AATTGAAGGG CAGCAGCCAA GCTGACACAG TTGACTTCAG TATTCTGTCA 
GTAGCTGGTA AGGCTTTGGG TGCAGCAGAC CTTCTGGAGC ACCACAGTGG TAGTCAGAGT 
CCTCTGCTGA CCACTGGAGA ATTAGGGAAA GAAAAAACTC AAAAGAGGGT GAAGGAAGGC 
AACGGTATGG GCGCTAGCAG TGGGTCAGGC CCGGGGTCAG ACAGCAAGCC AGGCAAGCGC 
AGCCGCACAC CTTCCAATGA TGGGAAGAGC AAAGATAAGC CTCCAAAGCG AAAGAAGGCA 
GACACGGAGG GAAAATCCCC ATCTCACAGT TCTAATAGAC CTTTCACCCC ACCCACCAGT 
ACAGGTGGGT CCAAATCCCC TGGCAGTTCA GGACGATGTC AGACGCCCCC TGGTGTCGCC 
ACACCGCCCA TTCCCAAGAT CACCATTCAG ATTCCTAAAG GAACAGTGAT GGTGGGCAAG 
CCTTCCTCGC ACAGTCAGTA CTCTAGCAGT GGTTCTGTGT CTTCCTCTGG CAGCAAAAGC 
CACCACAGTC ATTCTTCCTC CTCCTCTTCC TTATCTTCTG CTTCCACCTC AGGCAAGATG 
AAAAGCAGTA AATCAGAAGG CTCCTCAAGT GCCAAGCTCA GTGGCAGTAT GTATTCTAGC 
CAAGGGTCTT CTGGATCCAG CCAGTCCAAA AATTCATCTC AGACTGGGGG GAAGCCAGGC 
TCCTCTCCCA TTACCAAACA TGGACTGAGT AGTGGGTCTA GCAGCACCAA GATGAAACCT 
CAAGGCAAGC CATCCTCACT TATGAATCCT TCTATAAGTA AACCAAACAT ATCCCCTTCC 
CATTCAAGGC CTCCTGGAGG CTCAGATAAG CTTGCCTCTC CAATGAAGCC TGTTCCCGGG 
ACCCCCCCAT CCTCTAAAGC CAAGTCCCCT ATCAGTTCAG GTTCTGGTAG CTCTCATGTG 
TCAGGAACTA GTTCAAGCTC TGGTATGAAG TCTTCTTCAG GGTCAGGATC CTCAGGCTCA 
TTGTCTCAAA AAACTCCCCC AGCATCTAAT TCTTGTACAC CATCTTCCTC CTCATTTTCC 
TCAAGTGGTT CTTCCATGTC ATCCTCTCAG AATCAACATG GGAGTTCCAA AGGGAAATCT 
CCCAGTAGGA ATAAGAAGCC TTCTTTGACA GCTGTCATAG ATAAATTGAA GCATGGGGTG 
GTTACCAGTG GGCCTGGGGG TGAGGATCCA ATAGACAGTC AGATGGGTGC AAGCACAAAT 
TCCTCTAACC ATCCCATGTC CTCCAAACAT AACATGTCAG GAGGAGAATT CCAGAGCAAA 
CGTGAGAAAA GCGATAAAGA CAAATCAAAG GTTTCTACTT CTGGAGGATC AGTTGATTCC 
TCTAAGAAGA CTTCAGAGTC AAAAAATGTG GGGAGCACGG GTGTGGCAAA AATTATTATC 
AGCAAGCACG ATGGAGGCTC CCCGAGCATC AAAGCCAAAG TGACTCTACA GAAACCTGGA 
GATAGTGGTG GAGAAGGGCT CAGGCCACAG ATCGCCTCTT CAAAAAACTA TGGCTCTCCA 
TTGATCAGTG GTTCTACTCC AAAGCATGAA CGGGGTTCTC CCAGCCACAG TAAGTCGCCA 
GCATATACAC CTCAGAATGT GGACAGTGAA AGTGAGTCAG GCTCCTCCAT AGCAGAGAGA 
TCCTACCAGA ACAGCCCCAG CTCAGATGAT GGTGTCCGGC CACTCCCAGA ATACAGCACA 
GAGAAGCATA AGAAGCACAA AAAGGAAAAG AAAAAAGTAA AAGATAAAGA TAGAGACCGG 
GACAGAGACA AAGACAGAGA CAAGAAAAAA TCTCACAGCA TCAAGCCAGA GAATTGGTCT 
AAATCCCCCA TTTCTTCAGA TCAGACTTCA TCTATGACAA ATAACCCTAT CTTATCTGCA 
GACAGGCCTT CCAGGCTCAG CCCTGACTTT ATGATTGGGG AGGAAGATGA TGATCTTATG 
GATGTGGCTC TGATTGGCAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247461.1
CDS	340..5043
Translation	MSSLLDRLHAKFNQSRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNATPLDKILHGSVGYLTPRSGGHLMNMKYYASPSDLLHDTTASPITLHEKNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPADNKWTPSFSAVTSANSVDLPACFFLKFPQPIPVSKAFVQKLQNCTGIPLFETPPTYLPLYELITQFELSKDPDPLPLNHNMRFYAALPGQQHCYFLNKDAPLPDGQSLQGTLVSKITFQHPGRVPLILNMIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGSNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMCSGSNKTKKNKSSRVPPDKPKHQTEDDFQRELFSMDVDSQNPMFDVSMTADALDTPHITPAPSQCSTPPTTYPQPVSHPQPSIQRMGRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPPIGTPVRDSSSSGHSQSALFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGMPMLGGDNGESKLKGSSQADTVDFSILSVAGKALGAADLLEHHSGSQSPLLTTGELGKEKTQKRVKEGNGMGASSGSGPGSDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSNRPFTPPTSTGGSKSPGSSGRCQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYSSSGSVSSSGSKSHHSHSSSSSSLSSASTSGKMKSSKSEGSSSAKLSGSMYSSQGSSGSSQSKNSSQTGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSISKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGSSHVSGTSSSSGMKSSSGSGSSGSLSQKTPPASNSCTPSSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPIDSQMGASTNSSNHPMSSKHNMSGGEFQSKREKSDKDKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGDSGGEGLRPQIASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNVDSESESGSSIAERSYQNSPSSDDGVRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPENWSKSPISSDQTSSMTNNPILSADRPSRLSPDFMIGEEDDDLMDVALIGN

Target ORF information:

RefSeq Version	XM_006247399.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mediator complex subunit 1 (Med1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247399.3

ORF Insert Sequence:

ATGAGTTCTC TCTTGGACCG GCTCCATGCA AAATTTAACC AAAGTAGACC TTGGAGTGAG 
ACCATTAAGC TTGTGCGTCA AGTCATGGAG AAGAGGGTCG TAATGAGTTC TGGAGGACAT 
CAGCATTTGG TCAGCTGTTT GGAGACATTA CAGAAGGCTC TCAAAGTAAC ATCTTTGCCA 
GCAATGACTG ATCGTTTGGA ATCCATAGCC AGACAGAATG GACTGGGCTC TCATCTCAGT 
GCCAGTGGCA CTGAATGTTA CATCACGTCA GATATGTTCT ATGTGGAAGT GCAGTTAGAC 
CCTGCAGGAC AGCTTTGTGA TGTAAAAGTG GCTCACCATG GGGAGAATCC TGTGAGCTGT 
CCAGAGCTTG TGCAGCAGTT AAGGGAAAAG AATTTTGATG AGTTTTCTAA GCATCTTAAG 
GGTCTTGTTA ATCTGTATAA CCTTCCAGGA GACAACAAAC TGAAGACTAA GATGTATCTG 
GCTCTCCAGT CCTTAGAACA GGACCTTTCT AAAATGGCAA TTATGTACTG GAAGGCAACT 
AATGCTACTC CCTTGGATAA GATTCTTCAC GGAAGTGTTG GCTATCTCAC TCCACGGAGC 
GGGGGTCATT TAATGAACAT GAAATACTAT GCCTCTCCGT CTGACCTGCT GCATGATACA 
ACTGCCTCTC CTATCACTTT GCATGAAAAG AATGTTCCTC GGTCTTTGGG AATGAATGCC 
TCAGTGACAA TTGAAGGAAC CTCTGCTATG TACAAACTCC CAATTGCCCC ATTAATTATG 
GGGTCACACC CAGCTGACAA CAAATGGACC CCTTCTTTCT CTGCAGTCAC TAGTGCCAAC 
AGTGTTGATC TTCCTGCGTG TTTCTTCTTG AAATTTCCCC AGCCAATCCC AGTATCTAAA 
GCATTTGTTC AGAAACTGCA AAATTGCACG GGAATCCCGT TGTTTGAGAC TCCGCCTACT 
TACCTGCCCC TCTATGAACT CATCACTCAG TTTGAGCTGT CAAAGGATCC TGACCCTTTA 
CCTTTGAATC ACAACATGCG ATTTTACGCT GCTCTTCCAG GTCAGCAGCA CTGCTATTTT 
CTCAATAAAG ATGCTCCTCT TCCTGATGGC CAAAGCCTGC AGGGAACACT GGTCAGCAAA 
ATCACCTTCC AGCACCCTGG CCGAGTTCCT CTTATCTTGA ATATGATCAG ACACCAAGTG 
GCCTATAACA CCCTAATTGG AAGCTGTGTT AAAAGAACTA TTTTAAAAGA AGATTCTCCT 
GGGCTCCTCC AATTTGAAGT GTGTCCTCTT TCAGAATCTC GATTCAGTGT ATCTTTTCAG 
CACCCTGTGA ATGACTCCCT TGTGTGTGTG GTGATGGACG TGCAAGACTC AACACATGTG 
AGCTGTAAAC TCTACAAGGG GCTATCAGAT GCACTGATCT GTACAGACGA CTTCATTGCC 
AAAGTTGTTC AAAGATGTAT GTCCATCCCT GTGACGATGA GGGCTATTCG GAGGAAGGCT 
GAAACCATAC AGGCTGACAC GCCAGCACTG TCCCTCATTG CAGAGACAGT CGAAGACATG 
GTGAAAAAGA ACCTGCCCCC GGCTAGCAGC CCAGGGTATG GCATGACCAC AGGCAGCAAC 
CCAATGAGCG GTACCACCAC ACCAACCAAC ACCTTTCCGG GGGGTCCCAT TACCACCTTG 
TTTAATATGA GCATGAGCAT CAAAGATCGG CATGAGTCGG TGGGCCATGG GGAGGACTTC 
AGCAAGGTGT CTCAGAACCC AATTCTTACC AGTTTGTTGC AAATCACAGG GAACGGGGGG 
TCTACCATTG GCTCGAGTCC GACCCCTCCT CATCACACGC CGCCACCTGT CTCTTCGATG 
GCCGGCAACA CCAAGAACCA CCCGATGCTC ATGAACCTTC TTAAAGATAA CCCTGCCCAA 
GATTTCTCAA CCCTTTATGG AAGCAGCCCT TTAGAAAGGC AGAACTCCTC TTCCGGATCA 
CCCCGGATGG AAATGTGCTC GGGGAGCAAC AAGACCAAGA AAAATAAGTC ATCAAGAGTA 
CCACCGGACA AACCCAAGCA CCAGACCGAA GATGATTTTC AGAGGGAGCT CTTTTCAATG 
GATGTCGACT CACAGAACCC TATGTTCGAT GTCAGCATGA CAGCTGACGC GCTGGATACA 
CCTCATATCA CTCCAGCTCC AAGCCAGTGT AGTACGCCCC CAACAACGTA CCCACAGCCA 
GTGTCTCACC CCCAGCCCAG TATTCAAAGG ATGGGCCGAC TGTCCAGTTC AGACAGCATT 
GGCCCAGATG TAACTGATAT TCTTTCAGAC ATTGCAGAAG AAGCTTCAAA GCTTCCCAGC 
ACTAGTGATG ATTGCCCACC CATTGGTACC CCAGTTCGAG ATTCCTCGAG TTCTGGGCAT 
TCTCAGAGTG CCCTCTTTGA CTCTGATGTC TTTCAGACTA ATAACAATGA AAATCCATAC 
ACTGACCCGG CTGACCTTAT TGCAGATGCT GCTGGGAGCC CCAGTAGTGA TTCTCCTACC 
AATCATTTTT TCCCTGATGG AGTAGATTTC AATCCTGATT TGTTGAACAG CCAAAGCCAA 
AGTGGTTTTG GAGAGGAGTA TTTTGATGAA AGTAGTCAGA GTGGGGATAA TGATGATTTC 
AAAGGATTTG CATCTCAGGC ACTAAATACT TTGGGGATGC CAATGCTCGG AGGTGACAAT 
GGGGAGTCGA AATTGAAGGG CAGCAGCCAA GCTGACACAG TTGACTTCAG TATTCTGTCA 
GTAGCTGGTA AGGCTTTGGG TGCAGCAGAC CTTCTGGAGC ACCACAGTGG TAGTCAGAGT 
CCTCTGCTGA CCACTGGAGA ATTAGGGAAA GAAAAAACTC AAAAGAGGGT GAAGGAAGGC 
AACGGTATGG GCGCTAGCAG TGGGTCAGGC CCGGGGTCAG ACAGCAAGCC AGGCAAGCGC 
AGCCGCACAC CTTCCAATGA TGGGAAGAGC AAAGATAAGC CTCCAAAGCG AAAGAAGGCA 
GACACGGAGG GAAAATCCCC ATCTCACAGT TCTAATAGAC CTTTCACCCC ACCCACCAGT 
ACAGGTGGGT CCAAATCCCC TGGCAGTTCA GGACGATGTC AGACGCCCCC TGGTGTCGCC 
ACACCGCCCA TTCCCAAGAT CACCATTCAG ATTCCTAAAG GAACAGTGAT GGTGGGCAAG 
CCTTCCTCGC ACAGTCAGTA CTCTAGCAGT GGTTCTGTGT CTTCCTCTGG CAGCAAAAGC 
CACCACAGTC ATTCTTCCTC CTCCTCTTCC TTATCTTCTG CTTCCACCTC AGGCAAGATG 
AAAAGCAGTA AATCAGAAGG CTCCTCAAGT GCCAAGCTCA GTGGCAGTAT GTATTCTAGC 
CAAGGGTCTT CTGGATCCAG CCAGTCCAAA AATTCATCTC AGACTGGGGG GAAGCCAGGC 
TCCTCTCCCA TTACCAAACA TGGACTGAGT AGTGGGTCTA GCAGCACCAA GATGAAACCT 
CAAGGCAAGC CATCCTCACT TATGAATCCT TCTATAAGTA AACCAAACAT ATCCCCTTCC 
CATTCAAGGC CTCCTGGAGG CTCAGATAAG CTTGCCTCTC CAATGAAGCC TGTTCCCGGG 
ACCCCCCCAT CCTCTAAAGC CAAGTCCCCT ATCAGTTCAG GTTCTGGTAG CTCTCATGTG 
TCAGGAACTA GTTCAAGCTC TGGTATGAAG TCTTCTTCAG GGTCAGGATC CTCAGGCTCA 
TTGTCTCAAA AAACTCCCCC AGCATCTAAT TCTTGTACAC CATCTTCCTC CTCATTTTCC 
TCAAGTGGTT CTTCCATGTC ATCCTCTCAG AATCAACATG GGAGTTCCAA AGGGAAATCT 
CCCAGTAGGA ATAAGAAGCC TTCTTTGACA GCTGTCATAG ATAAATTGAA GCATGGGGTG 
GTTACCAGTG GGCCTGGGGG TGAGGATCCA ATAGACAGTC AGATGGGTGC AAGCACAAAT 
TCCTCTAACC ATCCCATGTC CTCCAAACAT AACATGTCAG GAGGAGAATT CCAGAGCAAA 
CGTGAGAAAA GCGATAAAGA CAAATCAAAG GTTTCTACTT CTGGAGGATC AGTTGATTCC 
TCTAAGAAGA CTTCAGAGTC AAAAAATGTG GGGAGCACGG GTGTGGCAAA AATTATTATC 
AGCAAGCACG ATGGAGGCTC CCCGAGCATC AAAGCCAAAG TGACTCTACA GAAACCTGGA 
GATAGTGGTG GAGAAGGGCT CAGGCCACAG ATCGCCTCTT CAAAAAACTA TGGCTCTCCA 
TTGATCAGTG GTTCTACTCC AAAGCATGAA CGGGGTTCTC CCAGCCACAG TAAGTCGCCA 
GCATATACAC CTCAGAATGT GGACAGTGAA AGTGAGTCAG GCTCCTCCAT AGCAGAGAGA 
TCCTACCAGA ACAGCCCCAG CTCAGATGAT GGTGTCCGGC CACTCCCAGA ATACAGCACA 
GAGAAGCATA AGAAGCACAA AAAGGAAAAG AAAAAAGTAA AAGATAAAGA TAGAGACCGG 
GACAGAGACA AAGACAGAGA CAAGAAAAAA TCTCACAGCA TCAAGCCAGA GAATTGGTCT 
AAATCCCCCA TTTCTTCAGA TCAGACTTCA TCTATGACAA ATAACCCTAT CTTATCTGCA 
GACAGGCCTT CCAGGCTCAG CCCTGACTTT ATGATTGGGG AGGAAGATGA TGATCTTATG 
GATGTGGCTC TGATTGGCAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med1 ( XM_006247398.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247460.1 Rattus norvegicus mediator complex subunit 1 (Med1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa33747
Clone ID Related Accession (Same CDS sequence)	XM_006247398.3
Accession Version	XM_006247398.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mediator of RNA polymerase II transcription subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247398.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCTC AGGGGGAAAC CGAGGAGTCA GAGAAGCTGA GTAAGATGAG TTCTCTCTTG 
GACCGGCTCC ATGCAAAATT TAACCAAAGT AGACCTTGGA GTGAGACCAT TAAGCTTGTG 
CGTCAAGTCA TGGAGAAGAG GGTCGTAATG AGTTCTGGAG GACATCAGCA TTTGGTCAGC 
TGTTTGGAGA CATTACAGAA GGCTCTCAAA GTAACATCTT TGCCAGCAAT GACTGATCGT 
TTGGAATCCA TAGCCAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA 
TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGACCCTGC AGGACAGCTT 
TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCAGA GCTTGTGCAG 
CAGTTAAGGG AAAAGAATTT TGATGAGTTT TCTAAGCATC TTAAGGGTCT TGTTAATCTG 
TATAACCTTC CAGGAGACAA CAAACTGAAG ACTAAGATGT ATCTGGCTCT CCAGTCCTTA 
GAACAGGACC TTTCTAAAAT GGCAATTATG TACTGGAAGG CAACTAATGC TACTCCCTTG 
GATAAGATTC TTCACGGAAG TGTTGGCTAT CTCACTCCAC GGAGCGGGGG TCATTTAATG 
AACATGAAAT ACTATGCCTC TCCGTCTGAC CTGCTGCATG ATACAACTGC CTCTCCTATC 
ACTTTGCATG AAAAGAATGT TCCTCGGTCT TTGGGAATGA ATGCCTCAGT GACAATTGAA 
GGAACCTCTG CTATGTACAA ACTCCCAATT GCCCCATTAA TTATGGGGTC ACACCCAGCT 
GACAACAAAT GGACCCCTTC TTTCTCTGCA GTCACTAGTG CCAACAGTGT TGATCTTCCT 
GCGTGTTTCT TCTTGAAATT TCCCCAGCCA ATCCCAGTAT CTAAAGCATT TGTTCAGAAA 
CTGCAAAATT GCACGGGAAT CCCGTTGTTT GAGACTCCGC CTACTTACCT GCCCCTCTAT 
GAACTCATCA CTCAGTTTGA GCTGTCAAAG GATCCTGACC CTTTACCTTT GAATCACAAC 
ATGCGATTTT ACGCTGCTCT TCCAGGTCAG CAGCACTGCT ATTTTCTCAA TAAAGATGCT 
CCTCTTCCTG ATGGCCAAAG CCTGCAGGGA ACACTGGTCA GCAAAATCAC CTTCCAGCAC 
CCTGGCCGAG TTCCTCTTAT CTTGAATATG ATCAGACACC AAGTGGCCTA TAACACCCTA 
ATTGGAAGCT GTGTTAAAAG AACTATTTTA AAAGAAGATT CTCCTGGGCT CCTCCAATTT 
GAAGTGTGTC CTCTTTCAGA ATCTCGATTC AGTGTATCTT TTCAGCACCC TGTGAATGAC 
TCCCTTGTGT GTGTGGTGAT GGACGTGCAA GACTCAACAC ATGTGAGCTG TAAACTCTAC 
AAGGGGCTAT CAGATGCACT GATCTGTACA GACGACTTCA TTGCCAAAGT TGTTCAAAGA 
TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AGGCTGAAAC CATACAGGCT 
GACACGCCAG CACTGTCCCT CATTGCAGAG ACAGTCGAAG ACATGGTGAA AAAGAACCTG 
CCCCCGGCTA GCAGCCCAGG GTATGGCATG ACCACAGGCA GCAACCCAAT GAGCGGTACC 
ACCACACCAA CCAACACCTT TCCGGGGGGT CCCATTACCA CCTTGTTTAA TATGAGCATG 
AGCATCAAAG ATCGGCATGA GTCGGTGGGC CATGGGGAGG ACTTCAGCAA GGTGTCTCAG 
AACCCAATTC TTACCAGTTT GTTGCAAATC ACAGGGAACG GGGGGTCTAC CATTGGCTCG 
AGTCCGACCC CTCCTCATCA CACGCCGCCA CCTGTCTCTT CGATGGCCGG CAACACCAAG 
AACCACCCGA TGCTCATGAA CCTTCTTAAA GATAACCCTG CCCAAGATTT CTCAACCCTT 
TATGGAAGCA GCCCTTTAGA AAGGCAGAAC TCCTCTTCCG GATCACCCCG GATGGAAATG 
TGCTCGGGGA GCAACAAGAC CAAGAAAAAT AAGTCATCAA GAGTACCACC GGACAAACCC 
AAGCACCAGA CCGAAGATGA TTTTCAGAGG GAGCTCTTTT CAATGGATGT CGACTCACAG 
AACCCTATGT TCGATGTCAG CATGACAGCT GACGCGCTGG ATACACCTCA TATCACTCCA 
GCTCCAAGCC AGTGTAGTAC GCCCCCAACA ACGTACCCAC AGCCAGTGTC TCACCCCCAG 
CCCAGTATTC AAAGGATGGG CCGACTGTCC AGTTCAGACA GCATTGGCCC AGATGTAACT 
GATATTCTTT CAGACATTGC AGAAGAAGCT TCAAAGCTTC CCAGCACTAG TGATGATTGC 
CCACCCATTG GTACCCCAGT TCGAGATTCC TCGAGTTCTG GGCATTCTCA GAGTGCCCTC 
TTTGACTCTG ATGTCTTTCA GACTAATAAC AATGAAAATC CATACACTGA CCCGGCTGAC 
CTTATTGCAG ATGCTGCTGG GAGCCCCAGT AGTGATTCTC CTACCAATCA TTTTTTCCCT 
GATGGAGTAG ATTTCAATCC TGATTTGTTG AACAGCCAAA GCCAAAGTGG TTTTGGAGAG 
GAGTATTTTG ATGAAAGTAG TCAGAGTGGG GATAATGATG ATTTCAAAGG ATTTGCATCT 
CAGGCACTAA ATACTTTGGG GATGCCAATG CTCGGAGGTG ACAATGGGGA GTCGAAATTG 
AAGGGCAGCA GCCAAGCTGA CACAGTTGAC TTCAGTATTC TGTCAGTAGC TGGTAAGGCT 
TTGGGTGCAG CAGACCTTCT GGAGCACCAC AGTGGTAGTC AGAGTCCTCT GCTGACCACT 
GGAGAATTAG GGAAAGAAAA AACTCAAAAG AGGGTGAAGG AAGGCAACGG TATGGGCGCT 
AGCAGTGGGT CAGGCCCGGG GTCAGACAGC AAGCCAGGCA AGCGCAGCCG CACACCTTCC 
AATGATGGGA AGAGCAAAGA TAAGCCTCCA AAGCGAAAGA AGGCAGACAC GGAGGGAAAA 
TCCCCATCTC ACAGTTCTAA TAGACCTTTC ACCCCACCCA CCAGTACAGG TGGGTCCAAA 
TCCCCTGGCA GTTCAGGACG ATGTCAGACG CCCCCTGGTG TCGCCACACC GCCCATTCCC 
AAGATCACCA TTCAGATTCC TAAAGGAACA GTGATGGTGG GCAAGCCTTC CTCGCACAGT 
CAGTACTCTA GCAGTGGTTC TGTGTCTTCC TCTGGCAGCA AAAGCCACCA CAGTCATTCT 
TCCTCCTCCT CTTCCTTATC TTCTGCTTCC ACCTCAGGCA AGATGAAAAG CAGTAAATCA 
GAAGGCTCCT CAAGTGCCAA GCTCAGTGGC AGTATGTATT CTAGCCAAGG GTCTTCTGGA 
TCCAGCCAGT CCAAAAATTC ATCTCAGACT GGGGGGAAGC CAGGCTCCTC TCCCATTACC 
AAACATGGAC TGAGTAGTGG GTCTAGCAGC ACCAAGATGA AACCTCAAGG CAAGCCATCC 
TCACTTATGA ATCCTTCTAT AAGTAAACCA AACATATCCC CTTCCCATTC AAGGCCTCCT 
GGAGGCTCAG ATAAGCTTGC CTCTCCAATG AAGCCTGTTC CCGGGACCCC CCCATCCTCT 
AAAGCCAAGT CCCCTATCAG TTCAGGTTCT GGTAGCTCTC ATGTGTCAGG AACTAGTTCA 
AGCTCTGGTA TGAAGTCTTC TTCAGGGTCA GGATCCTCAG GCTCATTGTC TCAAAAAACT 
CCCCCAGCAT CTAATTCTTG TACACCATCT TCCTCCTCAT TTTCCTCAAG TGGTTCTTCC 
ATGTCATCCT CTCAGAATCA ACATGGGAGT TCCAAAGGGA AATCTCCCAG TAGGAATAAG 
AAGCCTTCTT TGACAGCTGT CATAGATAAA TTGAAGCATG GGGTGGTTAC CAGTGGGCCT 
GGGGGTGAGG ATCCAATAGA CAGTCAGATG GGTGCAAGCA CAAATTCCTC TAACCATCCC 
ATGTCCTCCA AACATAACAT GTCAGGAGGA GAATTCCAGA GCAAACGTGA GAAAAGCGAT 
AAAGACAAAT CAAAGGTTTC TACTTCTGGA GGATCAGTTG ATTCCTCTAA GAAGACTTCA 
GAGTCAAAAA ATGTGGGGAG CACGGGTGTG GCAAAAATTA TTATCAGCAA GCACGATGGA 
GGCTCCCCGA GCATCAAAGC CAAAGTGACT CTACAGAAAC CTGGAGATAG TGGTGGAGAA 
GGGCTCAGGC CACAGATCGC CTCTTCAAAA AACTATGGCT CTCCATTGAT CAGTGGTTCT 
ACTCCAAAGC ATGAACGGGG TTCTCCCAGC CACAGTAAGT CGCCAGCATA TACACCTCAG 
AATGTGGACA GTGAAAGTGA GTCAGGCTCC TCCATAGCAG AGAGATCCTA CCAGAACAGC 
CCCAGCTCAG ATGATGGTGT CCGGCCACTC CCAGAATACA GCACAGAGAA GCATAAGAAG 
CACAAAAAGG AAAAGAAAAA AGTAAAAGAT AAAGATAGAG ACCGGGACAG AGACAAAGAC 
AGAGACAAGA AAAAATCTCA CAGCATCAAG CCAGAGAATT GGTCTAAATC CCCCATTTCT 
TCAGATCAGA CTTCATCTAT GACAAATAAC CCTATCTTAT CTGCAGACAG GCCTTCCAGG 
CTCAGCCCTG ACTTTATGAT TGGGGAGGAA GATGATGATC TTATGGATGT GGCTCTGATT 
GGCAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247460.1
CDS	220..4968
Translation	MKAQGETEESEKLSKMSSLLDRLHAKFNQSRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNATPLDKILHGSVGYLTPRSGGHLMNMKYYASPSDLLHDTTASPITLHEKNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPADNKWTPSFSAVTSANSVDLPACFFLKFPQPIPVSKAFVQKLQNCTGIPLFETPPTYLPLYELITQFELSKDPDPLPLNHNMRFYAALPGQQHCYFLNKDAPLPDGQSLQGTLVSKITFQHPGRVPLILNMIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGSNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMCSGSNKTKKNKSSRVPPDKPKHQTEDDFQRELFSMDVDSQNPMFDVSMTADALDTPHITPAPSQCSTPPTTYPQPVSHPQPSIQRMGRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPPIGTPVRDSSSSGHSQSALFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGMPMLGGDNGESKLKGSSQADTVDFSILSVAGKALGAADLLEHHSGSQSPLLTTGELGKEKTQKRVKEGNGMGASSGSGPGSDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSNRPFTPPTSTGGSKSPGSSGRCQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYSSSGSVSSSGSKSHHSHSSSSSSLSSASTSGKMKSSKSEGSSSAKLSGSMYSSQGSSGSSQSKNSSQTGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSISKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGSSHVSGTSSSSGMKSSSGSGSSGSLSQKTPPASNSCTPSSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPIDSQMGASTNSSNHPMSSKHNMSGGEFQSKREKSDKDKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGDSGGEGLRPQIASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNVDSESESGSSIAERSYQNSPSSDDGVRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPENWSKSPISSDQTSSMTNNPILSADRPSRLSPDFMIGEEDDDLMDVALIGN

Target ORF information:

RefSeq Version	XM_006247398.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mediator complex subunit 1 (Med1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247398.3

ORF Insert Sequence:

ATGAAGGCTC AGGGGGAAAC CGAGGAGTCA GAGAAGCTGA GTAAGATGAG TTCTCTCTTG 
GACCGGCTCC ATGCAAAATT TAACCAAAGT AGACCTTGGA GTGAGACCAT TAAGCTTGTG 
CGTCAAGTCA TGGAGAAGAG GGTCGTAATG AGTTCTGGAG GACATCAGCA TTTGGTCAGC 
TGTTTGGAGA CATTACAGAA GGCTCTCAAA GTAACATCTT TGCCAGCAAT GACTGATCGT 
TTGGAATCCA TAGCCAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA 
TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGACCCTGC AGGACAGCTT 
TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCAGA GCTTGTGCAG 
CAGTTAAGGG AAAAGAATTT TGATGAGTTT TCTAAGCATC TTAAGGGTCT TGTTAATCTG 
TATAACCTTC CAGGAGACAA CAAACTGAAG ACTAAGATGT ATCTGGCTCT CCAGTCCTTA 
GAACAGGACC TTTCTAAAAT GGCAATTATG TACTGGAAGG CAACTAATGC TACTCCCTTG 
GATAAGATTC TTCACGGAAG TGTTGGCTAT CTCACTCCAC GGAGCGGGGG TCATTTAATG 
AACATGAAAT ACTATGCCTC TCCGTCTGAC CTGCTGCATG ATACAACTGC CTCTCCTATC 
ACTTTGCATG AAAAGAATGT TCCTCGGTCT TTGGGAATGA ATGCCTCAGT GACAATTGAA 
GGAACCTCTG CTATGTACAA ACTCCCAATT GCCCCATTAA TTATGGGGTC ACACCCAGCT 
GACAACAAAT GGACCCCTTC TTTCTCTGCA GTCACTAGTG CCAACAGTGT TGATCTTCCT 
GCGTGTTTCT TCTTGAAATT TCCCCAGCCA ATCCCAGTAT CTAAAGCATT TGTTCAGAAA 
CTGCAAAATT GCACGGGAAT CCCGTTGTTT GAGACTCCGC CTACTTACCT GCCCCTCTAT 
GAACTCATCA CTCAGTTTGA GCTGTCAAAG GATCCTGACC CTTTACCTTT GAATCACAAC 
ATGCGATTTT ACGCTGCTCT TCCAGGTCAG CAGCACTGCT ATTTTCTCAA TAAAGATGCT 
CCTCTTCCTG ATGGCCAAAG CCTGCAGGGA ACACTGGTCA GCAAAATCAC CTTCCAGCAC 
CCTGGCCGAG TTCCTCTTAT CTTGAATATG ATCAGACACC AAGTGGCCTA TAACACCCTA 
ATTGGAAGCT GTGTTAAAAG AACTATTTTA AAAGAAGATT CTCCTGGGCT CCTCCAATTT 
GAAGTGTGTC CTCTTTCAGA ATCTCGATTC AGTGTATCTT TTCAGCACCC TGTGAATGAC 
TCCCTTGTGT GTGTGGTGAT GGACGTGCAA GACTCAACAC ATGTGAGCTG TAAACTCTAC 
AAGGGGCTAT CAGATGCACT GATCTGTACA GACGACTTCA TTGCCAAAGT TGTTCAAAGA 
TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AGGCTGAAAC CATACAGGCT 
GACACGCCAG CACTGTCCCT CATTGCAGAG ACAGTCGAAG ACATGGTGAA AAAGAACCTG 
CCCCCGGCTA GCAGCCCAGG GTATGGCATG ACCACAGGCA GCAACCCAAT GAGCGGTACC 
ACCACACCAA CCAACACCTT TCCGGGGGGT CCCATTACCA CCTTGTTTAA TATGAGCATG 
AGCATCAAAG ATCGGCATGA GTCGGTGGGC CATGGGGAGG ACTTCAGCAA GGTGTCTCAG 
AACCCAATTC TTACCAGTTT GTTGCAAATC ACAGGGAACG GGGGGTCTAC CATTGGCTCG 
AGTCCGACCC CTCCTCATCA CACGCCGCCA CCTGTCTCTT CGATGGCCGG CAACACCAAG 
AACCACCCGA TGCTCATGAA CCTTCTTAAA GATAACCCTG CCCAAGATTT CTCAACCCTT 
TATGGAAGCA GCCCTTTAGA AAGGCAGAAC TCCTCTTCCG GATCACCCCG GATGGAAATG 
TGCTCGGGGA GCAACAAGAC CAAGAAAAAT AAGTCATCAA GAGTACCACC GGACAAACCC 
AAGCACCAGA CCGAAGATGA TTTTCAGAGG GAGCTCTTTT CAATGGATGT CGACTCACAG 
AACCCTATGT TCGATGTCAG CATGACAGCT GACGCGCTGG ATACACCTCA TATCACTCCA 
GCTCCAAGCC AGTGTAGTAC GCCCCCAACA ACGTACCCAC AGCCAGTGTC TCACCCCCAG 
CCCAGTATTC AAAGGATGGG CCGACTGTCC AGTTCAGACA GCATTGGCCC AGATGTAACT 
GATATTCTTT CAGACATTGC AGAAGAAGCT TCAAAGCTTC CCAGCACTAG TGATGATTGC 
CCACCCATTG GTACCCCAGT TCGAGATTCC TCGAGTTCTG GGCATTCTCA GAGTGCCCTC 
TTTGACTCTG ATGTCTTTCA GACTAATAAC AATGAAAATC CATACACTGA CCCGGCTGAC 
CTTATTGCAG ATGCTGCTGG GAGCCCCAGT AGTGATTCTC CTACCAATCA TTTTTTCCCT 
GATGGAGTAG ATTTCAATCC TGATTTGTTG AACAGCCAAA GCCAAAGTGG TTTTGGAGAG 
GAGTATTTTG ATGAAAGTAG TCAGAGTGGG GATAATGATG ATTTCAAAGG ATTTGCATCT 
CAGGCACTAA ATACTTTGGG GATGCCAATG CTCGGAGGTG ACAATGGGGA GTCGAAATTG 
AAGGGCAGCA GCCAAGCTGA CACAGTTGAC TTCAGTATTC TGTCAGTAGC TGGTAAGGCT 
TTGGGTGCAG CAGACCTTCT GGAGCACCAC AGTGGTAGTC AGAGTCCTCT GCTGACCACT 
GGAGAATTAG GGAAAGAAAA AACTCAAAAG AGGGTGAAGG AAGGCAACGG TATGGGCGCT 
AGCAGTGGGT CAGGCCCGGG GTCAGACAGC AAGCCAGGCA AGCGCAGCCG CACACCTTCC 
AATGATGGGA AGAGCAAAGA TAAGCCTCCA AAGCGAAAGA AGGCAGACAC GGAGGGAAAA 
TCCCCATCTC ACAGTTCTAA TAGACCTTTC ACCCCACCCA CCAGTACAGG TGGGTCCAAA 
TCCCCTGGCA GTTCAGGACG ATGTCAGACG CCCCCTGGTG TCGCCACACC GCCCATTCCC 
AAGATCACCA TTCAGATTCC TAAAGGAACA GTGATGGTGG GCAAGCCTTC CTCGCACAGT 
CAGTACTCTA GCAGTGGTTC TGTGTCTTCC TCTGGCAGCA AAAGCCACCA CAGTCATTCT 
TCCTCCTCCT CTTCCTTATC TTCTGCTTCC ACCTCAGGCA AGATGAAAAG CAGTAAATCA 
GAAGGCTCCT CAAGTGCCAA GCTCAGTGGC AGTATGTATT CTAGCCAAGG GTCTTCTGGA 
TCCAGCCAGT CCAAAAATTC ATCTCAGACT GGGGGGAAGC CAGGCTCCTC TCCCATTACC 
AAACATGGAC TGAGTAGTGG GTCTAGCAGC ACCAAGATGA AACCTCAAGG CAAGCCATCC 
TCACTTATGA ATCCTTCTAT AAGTAAACCA AACATATCCC CTTCCCATTC AAGGCCTCCT 
GGAGGCTCAG ATAAGCTTGC CTCTCCAATG AAGCCTGTTC CCGGGACCCC CCCATCCTCT 
AAAGCCAAGT CCCCTATCAG TTCAGGTTCT GGTAGCTCTC ATGTGTCAGG AACTAGTTCA 
AGCTCTGGTA TGAAGTCTTC TTCAGGGTCA GGATCCTCAG GCTCATTGTC TCAAAAAACT 
CCCCCAGCAT CTAATTCTTG TACACCATCT TCCTCCTCAT TTTCCTCAAG TGGTTCTTCC 
ATGTCATCCT CTCAGAATCA ACATGGGAGT TCCAAAGGGA AATCTCCCAG TAGGAATAAG 
AAGCCTTCTT TGACAGCTGT CATAGATAAA TTGAAGCATG GGGTGGTTAC CAGTGGGCCT 
GGGGGTGAGG ATCCAATAGA CAGTCAGATG GGTGCAAGCA CAAATTCCTC TAACCATCCC 
ATGTCCTCCA AACATAACAT GTCAGGAGGA GAATTCCAGA GCAAACGTGA GAAAAGCGAT 
AAAGACAAAT CAAAGGTTTC TACTTCTGGA GGATCAGTTG ATTCCTCTAA GAAGACTTCA 
GAGTCAAAAA ATGTGGGGAG CACGGGTGTG GCAAAAATTA TTATCAGCAA GCACGATGGA 
GGCTCCCCGA GCATCAAAGC CAAAGTGACT CTACAGAAAC CTGGAGATAG TGGTGGAGAA 
GGGCTCAGGC CACAGATCGC CTCTTCAAAA AACTATGGCT CTCCATTGAT CAGTGGTTCT 
ACTCCAAAGC ATGAACGGGG TTCTCCCAGC CACAGTAAGT CGCCAGCATA TACACCTCAG 
AATGTGGACA GTGAAAGTGA GTCAGGCTCC TCCATAGCAG AGAGATCCTA CCAGAACAGC 
CCCAGCTCAG ATGATGGTGT CCGGCCACTC CCAGAATACA GCACAGAGAA GCATAAGAAG 
CACAAAAAGG AAAAGAAAAA AGTAAAAGAT AAAGATAGAG ACCGGGACAG AGACAAAGAC 
AGAGACAAGA AAAAATCTCA CAGCATCAAG CCAGAGAATT GGTCTAAATC CCCCATTTCT 
TCAGATCAGA CTTCATCTAT GACAAATAAC CCTATCTTAT CTGCAGACAG GCCTTCCAGG 
CTCAGCCCTG ACTTTATGAT TGGGGAGGAA GATGATGATC TTATGGATGT GGCTCTGATT 
GGCAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cardiomyocyte-Specific Ablation of Med1 Subunit of the Mediator Complex Causes Lethal Dilated Cardiomyopathy in Mice.
PloS one11(8)e0160755(2016)
Jia Y,Chang HC,Schipma MJ,Liu J,Shete V,Liu N,Sato T,Thorp EB,Barger PM,Zhu YJ,Viswakarma N,Kanwar YS,Ardehali H,Thimmapaya B,Reddy JK

CCAR1/CoCoA pair-mediated recruitment of the Mediator defines a novel pathway for GATA1 function.
Genes to cells : devoted to molecular & cellular mechanisms19(1)28-51(2014 Jan)
Mizuta S,Minami T,Fujita H,Kaminaga C,Matsui K,Ishino R,Fujita A,Oda K,Kawai A,Hasegawa N,Urahama N,Roeder RG,Ito M

The Mediator complex and transcription regulation.
Critical reviews in biochemistry and molecular biology48(6)575-608(2013 Nov-Dec)
Poss ZC,Ebmeier CC,Taatjes DJ

Defective transcription initiation causes postnatal growth failure in a mouse model of nucleotide excision repair (NER) progeria.
Proceedings of the National Academy of Sciences of the United States of America109(8)2995-3000(2012 Feb)
Kamileri I,Karakasilioti I,Sideri A,Kosteas T,Tatarakis A,Talianidis I,Garinis GA

Specific erythroid-lineage defect in mice conditionally deficient for Mediator subunit Med1.
Proceedings of the National Academy of Sciences of the United States of America107(50)21541-6(2010 Dec)
Stumpf M,Yue X,Schmitz S,Luche H,Reddy JK,Borggrefe T

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Med1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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