MED1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED1
Entrez Gene ID	5469
Full Name	mediator complex subunit 1
Synonyms	CRSP1,CRSP200,DRIP205,DRIP230,PBP,PPARBP,PPARGBP,RB18A,TRAP220,TRIP2
General protein information	Preferred Names mediator complex subunit 1 Names mediator of RNA polymerase II transcription subunit 1 ARC205 PPAR-binding protein PPARG binding protein TR-interacting protein 2 TRIP-2 activator-recruited cofactor 205 kDa component p53 regulatory protein RB18A peroxisome proliferator-activated receptor-binding protein thyroid hormone receptor-associated protein complex 220 kDa component thyroid hormone receptor-associated protein complex component TRAP220 thyroid receptor interacting protein 2 vitamin D receptor-interacting protein 230 kD vitamin D receptor-interacting protein complex component DRIP205
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q12
Summary	The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq, Jul 2008].
Disorder MIM:	604311

mRNA and Protein(s)

mRNA	Protein	Name
XM_006721957.1	XP_006722020.1	mediator of RNA polymerase II transcription subunit 1 isoform X3
XM_017024779.1	XP_016880268.1	mediator of RNA polymerase II transcription subunit 1 isoform X2
XM_017024779.2	XP_016880268.1	mediator of RNA polymerase II transcription subunit 1 isoform X2
NM_004774.4	NP_004765.2	mediator of RNA polymerase II transcription subunit 1
NM_004774.3	NP_004765.2	mediator of RNA polymerase II transcription subunit 1
XM_005257465.3	XP_005257522.1	mediator of RNA polymerase II transcription subunit 1 isoform X1
XM_006721957.2	XP_006722020.1	mediator of RNA polymerase II transcription subunit 1 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MED1	XP_001172406.1
Canis lupus familiaris (dog)	MED1	XP_864009.2
Rattus norvegicus (Norway rat)	Med1	NP_001127833.1
Gallus gallus (chicken)	MED1	XP_418125.3
Bos taurus (cattle)	MED1	NP_001192805.1
Mus musculus (house mouse)	Med1	NP_001073587.1
Homo sapiens (human)	MED1	NP_004765.2
Xenopus tropicalis (tropical clawed frog)	med1	NP_001090727.1

Gene Ontology
Bibliography

Related articles in PubMed

HER2-Driven Breast Tumorigenesis Relies upon Interactions of the Estrogen Receptor with Coactivator MED1.
Yang Y, Leonard M, Zhang Y, Zhao D, Mahmoud C, Khan S, Wang J, Lower EE, Zhang X
Cancer research78(2)422-435(2018 Jan)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In a cohort of youth at risk for bipolar disorder, pathway analysis showed an enrichment of the glucocorticoid receptor (GR) pathway with the genes MED1, HSPA1L, GTF2A1 and TAF15, which might underlie the previously reported role of stress response in the risk for bipolar disorder in vulnerable populations.
Title: Integrated transcriptome and methylome analysis in youth at high risk for bipolar disorder: a preliminary analysis.

modulation of ESR1-MED1 interactions by cAMP signaling plays a critical role in human decidualization.
Title: Roles of Estrogen Receptor-? and the Coactivator MED1 During Human Endometrial Decidualization.

MED1 is required for optimal PRDM16-induced Ucp1 expression
Title: PRDM16 enhances nuclear receptor-dependent transcription of the brown fat-specific Ucp1 gene through interactions with Mediator subunit MED1.

Our data indicate that MED1 serves as a key mediator in ARv567es induced gene expression
Title: MED1 mediates androgen receptor splice variant induced gene expression in the absence of ligand.

Results show that miR-1 is downregulated in osteosarcoma cells but both of its targets Med1 and Med31 were overexpressed suggesting that MiR-1 plays an important role on the proliferation of osteosarcoma cells through regulation of Med1 and Med31.
Title: MicroRNA-1 functions as a potential tumor suppressor in osteosarcoma by targeting Med1 and Med31.

The following MED1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17285	NM_004774.4 Latest version!	Homo sapiens mediator complex subunit 1 (MED1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu50485	XM_006721957.2 Latest version!	Homo sapiens mediator complex subunit 1 (MED1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu86938	XM_017024779.2 Latest version!	Homo sapiens mediator complex subunit 1 (MED1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
OHu50484	XM_005257465.3 Latest version!	Homo sapiens mediator complex subunit 1 (MED1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$727.30 $1039.00
View Old Accession Versions >>
OHu86938	XM_017024779.1	Homo sapiens mediator complex subunit 1 (MED1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
OHu17285	NM_004774.3	Homo sapiens mediator complex subunit 1 (MED1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu50485	XM_006721957.1	Homo sapiens mediator complex subunit 1 (MED1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED1 ( NM_004774.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004765.2 Homo sapiens mediator complex subunit 1 (MED1), mRNA.

Price & Availability↑

CloneID	OHu17285
Clone ID Related Accession (Same CDS sequence)	NM_004774.4 , NM_004774.3
Accession Version	NM_004774.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP357374.1, BC131783.1 and AC005288.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_004774.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y13467.1, BC131783.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000300651.11/ ENSP00000300651.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAAGCTC AGGGGGAAAC CGAGGAGTCA GAAAAGCTGA GTAAGATGAG TTCTCTCCTG 
GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCCTGGA GTGAAACCAT TAAGCTTGTG 
CGTCAAGTCA TGGAGAAGAG GGTTGTGATG AGTTCTGGAG GGCATCAACA TTTGGTCAGC 
TGTTTGGAGA CATTGCAGAA GGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGATCGT 
TTGGAGTCCA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA 
TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT 
TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCGGA GCTTGTACAG 
CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGCCT TGTTAATCTG 
TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA 
GAACAAGATC TTTCTAAAAT GGCAATTATG TACTGGAAAG CAACTAATGC TGGTCCCTTG 
GATAAGATTC TTCATGGAAG TGTTGGCTAT CTCACACCAA GGAGTGGGGG TCATTTAATG 
AACCTGAAGT ACTATGTCTC TCCTTCTGAC CTACTGGATG ACAAGACTGC ATCTCCCATC 
ATTTTGCATG AGAATAATGT TTCTCGATCT TTGGGCATGA ATGCATCAGT GACAATTGAA 
GGAACATCTG CTGTGTACAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT 
GACAATAAAT GGACCCCTTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT 
GCCTGTTTCT TCTTGAAATT TCCCCAGCCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA 
CTGCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGC ACCCCTGTAT 
GAACTGATCA CTCAGTTTGA GCTATCAAAG GACCCTGACC CCATACCTTT GAATCACAAC 
ATGAGATTTT ATGCTGCTCT TCCTGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT 
CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTTGTTA GCAAAATCAC CTTTCAGCAC 
CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACACC AAGTGGCCTA TAACACCCTC 
ATTGGAAGCT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT TCTCCAATTT 
GAAGTGTGTC CTCTCTCAGA GTCTCGTTTC AGCGTATCTT TTCAGCACCC TGTGAATGAC 
TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTCTAC 
AAAGGGCTGT CGGATGCACT GATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA 
TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAAGCC 
GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG 
CCCCCGGCTA GCAGCCCAGG GTATGGCATG ACCACAGGCA ACAACCCAAT GAGTGGTACC 
ACTACACCAA CCAACACCTT TCCGGGGGGT CCCATTACCA CCTTGTTTAA TATGAGCATG 
AGCATCAAAG ATCGGCATGA GTCGGTGGGC CATGGGGAGG ACTTCAGCAA GGTGTCTCAG 
AACCCAATTC TTACCAGTTT GTTGCAAATC ACAGGGAACG GGGGGTCTAC CATTGGCTCG 
AGTCCGACCC CTCCTCATCA CACGCCGCCA CCTGTCTCTT CGATGGCCGG CAACACCAAG 
AACCACCCGA TGCTCATGAA CCTTCTTAAA GATAATCCTG CCCAGGATTT CTCAACCCTT 
TATGGAAGCA GCCCTTTAGA AAGGCAGAAC TCCTCTTCCG GCTCACCCCG CATGGAAATA 
TGCTCGGGGA GCAACAAGAC CAAGAAAAAG AAGTCATCAA GATTACCACC TGAGAAACCA 
AAGCACCAGA CTGAAGATGA CTTTCAGAGG GAGCTATTTT CAATGGATGT TGACTCACAG 
AACCCTATCT TTGATGTCAA CATGACAGCT GACACGCTGG ATACGCCACA CATCACTCCA 
GCTCCAAGCC AGTGTAGCAC TCCCCCAACA ACTTACCCAC AACCAGTACC TCACCCCCAA 
CCCAGTATTC AAAGGATGGT CCGACTATCC AGTTCAGACA GCATTGGCCC AGATGTAACT 
GACATCCTTT CAGACATTGC AGAAGAAGCT TCTAAACTTC CCAGCACTAG TGATGATTGC 
CCAGCCATTG GCACCCCTCT TCGAGATTCT TCAAGCTCTG GGCATTCTCA GAGTACCCTG 
TTTGACTCTG ATGTCTTTCA AACTAACAAT AATGAAAATC CATACACTGA TCCAGCTGAT 
CTTATTGCAG ATGCTGCTGG AAGCCCCAGT AGTGACTCTC CTACCAATCA TTTTTTTCAT 
GATGGAGTAG ATTTCAATCC TGATTTATTG AACAGCCAGA GCCAAAGTGG TTTTGGAGAA 
GAATATTTTG ATGAAAGCAG CCAAAGTGGG GATAATGATG ATTTCAAAGG ATTTGCATCT 
CAGGCACTAA ATACTTTGGG GGTGCCAATG CTTGGAGGTG ATAATGGGGA GACCAAGTTT 
AAGGGCAATA ACCAAGCCGA CACAGTTGAT TTCAGTATTA TTTCAGTAGC CGGCAAAGCT 
TTAGCTCCTG CAGATCTTAT GGAGCATCAC AGTGGTAGTC AGGGTCCTTT ACTGACCACT 
GGGGACTTAG GGAAAGAAAA GACTCAAAAG AGGGTAAAGG AAGGCAATGG CACCAGTAAT 
AGTACTCTCT CGGGGCCCGG ATTAGACAGC AAACCAGGGA AGCGCAGTCG GACCCCTTCT 
AATGATGGGA AAAGCAAAGA TAAGCCTCCA AAGCGGAAGA AGGCAGACAC TGAGGGAAAG 
TCTCCATCTC ATAGTTCTTC TAACAGACCT TTTACCCCAC CTACCAGTAC AGGTGGATCT 
AAATCGCCAG GCAGTGCAGG AAGATCTCAG ACTCCCCCAG GTGTTGCCAC ACCACCCATT 
CCCAAAATCA CTATTCAGAT TCCTAAGGGA ACAGTGATGG TGGGCAAGCC TTCCTCTCAC 
AGTCAGTATA CCAGCAGTGG TTCTGTGTCT TCCTCAGGCA GCAAAAGCCA CCATAGCCAT 
TCTTCCTCCT CTTCCTCATC TGCTTCCACC TCAGGGAAGA TGAAAAGCAG TAAATCAGAA 
GGTTCATCAA GTTCCAAGTT AAGTAGCAGT ATGTATTCTA GCCAGGGGTC TTCTGGATCT 
AGCCAGTCCA AAAATTCATC CCAGTCTGGG GGGAAGCCAG GCTCCTCTCC CATAACCAAG 
CATGGACTGA GCAGTGGCTC TAGCAGCACC AAGATGAAAC CTCAAGGAAA GCCATCATCA 
CTTATGAATC CTTCTTTAAG TAAACCAAAC ATATCCCCTT CTCATTCAAG GCCACCTGGA 
GGCTCTGACA AGCTTGCCTC TCCAATGAAG CCTGTTCCTG GAACTCCTCC ATCCTCTAAA 
GCCAAGTCCC CTATCAGTTC AGGTTCTGGT GGTTCTCATA TGTCTGGAAC TAGTTCAAGC 
TCTGGCATGA AGTCATCTTC AGGGTTAGGA TCCTCAGGCT CGTTGTCCCA GAAAACTCCC 
CCATCATCTA ATTCCTGTAC GGCATCTTCC TCCTCCTTTT CCTCAAGTGG CTCTTCCATG 
TCATCCTCTC AGAACCAGCA TGGGAGTTCT AAAGGAAAAT CTCCCAGCAG AAACAAGAAG 
CCGTCCTTGA CAGCTGTCAT AGATAAACTG AAGCATGGGG TTGTCACCAG TGGCCCTGGG 
GGTGAAGACC CACTGGACGG CCAGATGGGG GTGAGCACAA ATTCTTCCAG CCATCCTATG 
TCCTCCAAAC ATAACATGTC AGGAGGAGAG TTTCAGGGCA AGCGTGAGAA AAGTGATAAA 
GACAAATCAA AGGTTTCCAC CTCCGGGAGT TCAGTGGATT CTTCTAAGAA GACCTCAGAG 
TCAAAAAATG TGGGGAGCAC AGGTGTGGCA AAAATTATCA TCAGTAAGCA TGATGGAGGC 
TCCCCTAGCA TTAAAGCCAA AGTGACTTTG CAGAAACCTG GGGAAAGTAG TGGAGAAGGG 
CTTAGGCCTC AAATGGCTTC TTCTAAAAAC TATGGCTCTC CACTCATCAG TGGTTCCACT 
CCAAAGCATG AGCGTGGCTC TCCCAGCCAT AGTAAGTCAC CAGCATATAC CCCCCAGAAT 
CTGGACAGTG AAAGTGAGTC AGGCTCCTCC ATAGCAGAGA AATCTTATCA GAATAGTCCC 
AGCTCAGACG ATGGTATCCG ACCACTTCCA GAATACAGCA CAGAGAAACA TAAGAAGCAC 
AAAAAGGAAA AGAAGAAAGT AAAAGACAAA GATAGGGACC GAGACCGGGA CAAAGACCGA 
GACAAGAAAA AATCTCATAG CATCAAGCCA GAGAGTTGGT CCAAATCACC CATCTCTTCA 
GACCAGTCCT TGTCTATGAC AAGTAACACA ATCTTATCTG CAGACAGACC CTCAAGGCTC 
AGCCCAGACT TTATGATTGG GGAGGAAGAT GATGATCTTA TGGATGTGGC CCTGATTGGG 
AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004765.2
CDS	202..4947
Translation	MKAQGETEESEKLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNAGPLDKILHGSVGYLTPRSGGHLMNLKYYVSPSDLLDDKTASPIILHENNVSRSLGMNASVTIEGTSAVYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYAPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEICSGSNKTKKKKSSRLPPEKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPAIGTPLRDSSSSGHSQSTLFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFHDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNNQADTVDFSIISVAGKALAPADLMEHHSGSQGPLLTTGDLGKEKTQKRVKEGNGTSNSTLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSAGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSASTSGKMKSSKSEGSSSSKLSSSMYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTSSSSGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPLDGQMGVSTNSSSHPMSSKHNMSGGEFQGKREKSDKDKSKVSTSGSSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSADRPSRLSPDFMIGEEDDDLMDVALIGN

Target ORF information:

RefSeq Version	NM_004774.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 1 (MED1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004774.4

ORF Insert Sequence:

ATGAAAGCTC AGGGGGAAAC CGAGGAGTCA GAAAAGCTGA GTAAGATGAG TTCTCTCCTG 
GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCCTGGA GTGAAACCAT TAAGCTTGTG 
CGTCAAGTCA TGGAGAAGAG GGTTGTGATG AGTTCTGGAG GGCATCAACA TTTGGTCAGC 
TGTTTGGAGA CATTGCAGAA GGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGATCGT 
TTGGAGTCCA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA 
TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT 
TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCGGA GCTTGTACAG 
CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGCCT TGTTAATCTG 
TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA 
GAACAAGATC TTTCTAAAAT GGCAATTATG TACTGGAAAG CAACTAATGC TGGTCCCTTG 
GATAAGATTC TTCATGGAAG TGTTGGCTAT CTCACACCAA GGAGTGGGGG TCATTTAATG 
AACCTGAAGT ACTATGTCTC TCCTTCTGAC CTACTGGATG ACAAGACTGC ATCTCCCATC 
ATTTTGCATG AGAATAATGT TTCTCGATCT TTGGGCATGA ATGCATCAGT GACAATTGAA 
GGAACATCTG CTGTGTACAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT 
GACAATAAAT GGACCCCTTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT 
GCCTGTTTCT TCTTGAAATT TCCCCAGCCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA 
CTGCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGC ACCCCTGTAT 
GAACTGATCA CTCAGTTTGA GCTATCAAAG GACCCTGACC CCATACCTTT GAATCACAAC 
ATGAGATTTT ATGCTGCTCT TCCTGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT 
CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTTGTTA GCAAAATCAC CTTTCAGCAC 
CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACACC AAGTGGCCTA TAACACCCTC 
ATTGGAAGCT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT TCTCCAATTT 
GAAGTGTGTC CTCTCTCAGA GTCTCGTTTC AGCGTATCTT TTCAGCACCC TGTGAATGAC 
TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTCTAC 
AAAGGGCTGT CGGATGCACT GATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA 
TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAAGCC 
GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG 
CCCCCGGCTA GCAGCCCAGG GTATGGCATG ACCACAGGCA ACAACCCAAT GAGTGGTACC 
ACTACACCAA CCAACACCTT TCCGGGGGGT CCCATTACCA CCTTGTTTAA TATGAGCATG 
AGCATCAAAG ATCGGCATGA GTCGGTGGGC CATGGGGAGG ACTTCAGCAA GGTGTCTCAG 
AACCCAATTC TTACCAGTTT GTTGCAAATC ACAGGGAACG GGGGGTCTAC CATTGGCTCG 
AGTCCGACCC CTCCTCATCA CACGCCGCCA CCTGTCTCTT CGATGGCCGG CAACACCAAG 
AACCACCCGA TGCTCATGAA CCTTCTTAAA GATAATCCTG CCCAGGATTT CTCAACCCTT 
TATGGAAGCA GCCCTTTAGA AAGGCAGAAC TCCTCTTCCG GCTCACCCCG CATGGAAATA 
TGCTCGGGGA GCAACAAGAC CAAGAAAAAG AAGTCATCAA GATTACCACC TGAGAAACCA 
AAGCACCAGA CTGAAGATGA CTTTCAGAGG GAGCTATTTT CAATGGATGT TGACTCACAG 
AACCCTATCT TTGATGTCAA CATGACAGCT GACACGCTGG ATACGCCACA CATCACTCCA 
GCTCCAAGCC AGTGTAGCAC TCCCCCAACA ACTTACCCAC AACCAGTACC TCACCCCCAA 
CCCAGTATTC AAAGGATGGT CCGACTATCC AGTTCAGACA GCATTGGCCC AGATGTAACT 
GACATCCTTT CAGACATTGC AGAAGAAGCT TCTAAACTTC CCAGCACTAG TGATGATTGC 
CCAGCCATTG GCACCCCTCT TCGAGATTCT TCAAGCTCTG GGCATTCTCA GAGTACCCTG 
TTTGACTCTG ATGTCTTTCA AACTAACAAT AATGAAAATC CATACACTGA TCCAGCTGAT 
CTTATTGCAG ATGCTGCTGG AAGCCCCAGT AGTGACTCTC CTACCAATCA TTTTTTTCAT 
GATGGAGTAG ATTTCAATCC TGATTTATTG AACAGCCAGA GCCAAAGTGG TTTTGGAGAA 
GAATATTTTG ATGAAAGCAG CCAAAGTGGG GATAATGATG ATTTCAAAGG ATTTGCATCT 
CAGGCACTAA ATACTTTGGG GGTGCCAATG CTTGGAGGTG ATAATGGGGA GACCAAGTTT 
AAGGGCAATA ACCAAGCCGA CACAGTTGAT TTCAGTATTA TTTCAGTAGC CGGCAAAGCT 
TTAGCTCCTG CAGATCTTAT GGAGCATCAC AGTGGTAGTC AGGGTCCTTT ACTGACCACT 
GGGGACTTAG GGAAAGAAAA GACTCAAAAG AGGGTAAAGG AAGGCAATGG CACCAGTAAT 
AGTACTCTCT CGGGGCCCGG ATTAGACAGC AAACCAGGGA AGCGCAGTCG GACCCCTTCT 
AATGATGGGA AAAGCAAAGA TAAGCCTCCA AAGCGGAAGA AGGCAGACAC TGAGGGAAAG 
TCTCCATCTC ATAGTTCTTC TAACAGACCT TTTACCCCAC CTACCAGTAC AGGTGGATCT 
AAATCGCCAG GCAGTGCAGG AAGATCTCAG ACTCCCCCAG GTGTTGCCAC ACCACCCATT 
CCCAAAATCA CTATTCAGAT TCCTAAGGGA ACAGTGATGG TGGGCAAGCC TTCCTCTCAC 
AGTCAGTATA CCAGCAGTGG TTCTGTGTCT TCCTCAGGCA GCAAAAGCCA CCATAGCCAT 
TCTTCCTCCT CTTCCTCATC TGCTTCCACC TCAGGGAAGA TGAAAAGCAG TAAATCAGAA 
GGTTCATCAA GTTCCAAGTT AAGTAGCAGT ATGTATTCTA GCCAGGGGTC TTCTGGATCT 
AGCCAGTCCA AAAATTCATC CCAGTCTGGG GGGAAGCCAG GCTCCTCTCC CATAACCAAG 
CATGGACTGA GCAGTGGCTC TAGCAGCACC AAGATGAAAC CTCAAGGAAA GCCATCATCA 
CTTATGAATC CTTCTTTAAG TAAACCAAAC ATATCCCCTT CTCATTCAAG GCCACCTGGA 
GGCTCTGACA AGCTTGCCTC TCCAATGAAG CCTGTTCCTG GAACTCCTCC ATCCTCTAAA 
GCCAAGTCCC CTATCAGTTC AGGTTCTGGT GGTTCTCATA TGTCTGGAAC TAGTTCAAGC 
TCTGGCATGA AGTCATCTTC AGGGTTAGGA TCCTCAGGCT CGTTGTCCCA GAAAACTCCC 
CCATCATCTA ATTCCTGTAC GGCATCTTCC TCCTCCTTTT CCTCAAGTGG CTCTTCCATG 
TCATCCTCTC AGAACCAGCA TGGGAGTTCT AAAGGAAAAT CTCCCAGCAG AAACAAGAAG 
CCGTCCTTGA CAGCTGTCAT AGATAAACTG AAGCATGGGG TTGTCACCAG TGGCCCTGGG 
GGTGAAGACC CACTGGACGG CCAGATGGGG GTGAGCACAA ATTCTTCCAG CCATCCTATG 
TCCTCCAAAC ATAACATGTC AGGAGGAGAG TTTCAGGGCA AGCGTGAGAA AAGTGATAAA 
GACAAATCAA AGGTTTCCAC CTCCGGGAGT TCAGTGGATT CTTCTAAGAA GACCTCAGAG 
TCAAAAAATG TGGGGAGCAC AGGTGTGGCA AAAATTATCA TCAGTAAGCA TGATGGAGGC 
TCCCCTAGCA TTAAAGCCAA AGTGACTTTG CAGAAACCTG GGGAAAGTAG TGGAGAAGGG 
CTTAGGCCTC AAATGGCTTC TTCTAAAAAC TATGGCTCTC CACTCATCAG TGGTTCCACT 
CCAAAGCATG AGCGTGGCTC TCCCAGCCAT AGTAAGTCAC CAGCATATAC CCCCCAGAAT 
CTGGACAGTG AAAGTGAGTC AGGCTCCTCC ATAGCAGAGA AATCTTATCA GAATAGTCCC 
AGCTCAGACG ATGGTATCCG ACCACTTCCA GAATACAGCA CAGAGAAACA TAAGAAGCAC 
AAAAAGGAAA AGAAGAAAGT AAAAGACAAA GATAGGGACC GAGACCGGGA CAAAGACCGA 
GACAAGAAAA AATCTCATAG CATCAAGCCA GAGAGTTGGT CCAAATCACC CATCTCTTCA 
GACCAGTCCT TGTCTATGAC AAGTAACACA ATCTTATCTG CAGACAGACC CTCAAGGCTC 
AGCCCAGACT TTATGATTGG GGAGGAAGAT GATGATCTTA TGGATGTGGC CCTGATTGGG 
AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED1 ( NM_004774.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004765.2 Homo sapiens mediator complex subunit 1 (MED1), mRNA.

Price & Availability↑

CloneID	OHu17285
Clone ID Related Accession (Same CDS sequence)	NM_004774.4 , NM_004774.3
Accession Version	NM_004774.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP357374.1, BC131783.1 and AC005288.1. This sequence is a reference standard in the RefSeqGene project. On Aug 7, 2007 this sequence version replaced NM_004774.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y13467.1, BC131783.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAGCTC AGGGGGAAAC CGAGGAGTCA GAAAAGCTGA GTAAGATGAG TTCTCTCCTG 
GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCCTGGA GTGAAACCAT TAAGCTTGTG 
CGTCAAGTCA TGGAGAAGAG GGTTGTGATG AGTTCTGGAG GGCATCAACA TTTGGTCAGC 
TGTTTGGAGA CATTGCAGAA GGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGATCGT 
TTGGAGTCCA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA 
TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT 
TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCGGA GCTTGTACAG 
CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGCCT TGTTAATCTG 
TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA 
GAACAAGATC TTTCTAAAAT GGCAATTATG TACTGGAAAG CAACTAATGC TGGTCCCTTG 
GATAAGATTC TTCATGGAAG TGTTGGCTAT CTCACACCAA GGAGTGGGGG TCATTTAATG 
AACCTGAAGT ACTATGTCTC TCCTTCTGAC CTACTGGATG ACAAGACTGC ATCTCCCATC 
ATTTTGCATG AGAATAATGT TTCTCGATCT TTGGGCATGA ATGCATCAGT GACAATTGAA 
GGAACATCTG CTGTGTACAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT 
GACAATAAAT GGACCCCTTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT 
GCCTGTTTCT TCTTGAAATT TCCCCAGCCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA 
CTGCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGC ACCCCTGTAT 
GAACTGATCA CTCAGTTTGA GCTATCAAAG GACCCTGACC CCATACCTTT GAATCACAAC 
ATGAGATTTT ATGCTGCTCT TCCTGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT 
CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTTGTTA GCAAAATCAC CTTTCAGCAC 
CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACACC AAGTGGCCTA TAACACCCTC 
ATTGGAAGCT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT TCTCCAATTT 
GAAGTGTGTC CTCTCTCAGA GTCTCGTTTC AGCGTATCTT TTCAGCACCC TGTGAATGAC 
TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTCTAC 
AAAGGGCTGT CGGATGCACT GATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA 
TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAAGCC 
GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG 
CCCCCGGCTA GCAGCCCAGG GTATGGCATG ACCACAGGCA ACAACCCAAT GAGTGGTACC 
ACTACACCAA CCAACACCTT TCCGGGGGGT CCCATTACCA CCTTGTTTAA TATGAGCATG 
AGCATCAAAG ATCGGCATGA GTCGGTGGGC CATGGGGAGG ACTTCAGCAA GGTGTCTCAG 
AACCCAATTC TTACCAGTTT GTTGCAAATC ACAGGGAACG GGGGGTCTAC CATTGGCTCG 
AGTCCGACCC CTCCTCATCA CACGCCGCCA CCTGTCTCTT CGATGGCCGG CAACACCAAG 
AACCACCCGA TGCTCATGAA CCTTCTTAAA GATAATCCTG CCCAGGATTT CTCAACCCTT 
TATGGAAGCA GCCCTTTAGA AAGGCAGAAC TCCTCTTCCG GCTCACCCCG CATGGAAATA 
TGCTCGGGGA GCAACAAGAC CAAGAAAAAG AAGTCATCAA GATTACCACC TGAGAAACCA 
AAGCACCAGA CTGAAGATGA CTTTCAGAGG GAGCTATTTT CAATGGATGT TGACTCACAG 
AACCCTATCT TTGATGTCAA CATGACAGCT GACACGCTGG ATACGCCACA CATCACTCCA 
GCTCCAAGCC AGTGTAGCAC TCCCCCAACA ACTTACCCAC AACCAGTACC TCACCCCCAA 
CCCAGTATTC AAAGGATGGT CCGACTATCC AGTTCAGACA GCATTGGCCC AGATGTAACT 
GACATCCTTT CAGACATTGC AGAAGAAGCT TCTAAACTTC CCAGCACTAG TGATGATTGC 
CCAGCCATTG GCACCCCTCT TCGAGATTCT TCAAGCTCTG GGCATTCTCA GAGTACCCTG 
TTTGACTCTG ATGTCTTTCA AACTAACAAT AATGAAAATC CATACACTGA TCCAGCTGAT 
CTTATTGCAG ATGCTGCTGG AAGCCCCAGT AGTGACTCTC CTACCAATCA TTTTTTTCAT 
GATGGAGTAG ATTTCAATCC TGATTTATTG AACAGCCAGA GCCAAAGTGG TTTTGGAGAA 
GAATATTTTG ATGAAAGCAG CCAAAGTGGG GATAATGATG ATTTCAAAGG ATTTGCATCT 
CAGGCACTAA ATACTTTGGG GGTGCCAATG CTTGGAGGTG ATAATGGGGA GACCAAGTTT 
AAGGGCAATA ACCAAGCCGA CACAGTTGAT TTCAGTATTA TTTCAGTAGC CGGCAAAGCT 
TTAGCTCCTG CAGATCTTAT GGAGCATCAC AGTGGTAGTC AGGGTCCTTT ACTGACCACT 
GGGGACTTAG GGAAAGAAAA GACTCAAAAG AGGGTAAAGG AAGGCAATGG CACCAGTAAT 
AGTACTCTCT CGGGGCCCGG ATTAGACAGC AAACCAGGGA AGCGCAGTCG GACCCCTTCT 
AATGATGGGA AAAGCAAAGA TAAGCCTCCA AAGCGGAAGA AGGCAGACAC TGAGGGAAAG 
TCTCCATCTC ATAGTTCTTC TAACAGACCT TTTACCCCAC CTACCAGTAC AGGTGGATCT 
AAATCGCCAG GCAGTGCAGG AAGATCTCAG ACTCCCCCAG GTGTTGCCAC ACCACCCATT 
CCCAAAATCA CTATTCAGAT TCCTAAGGGA ACAGTGATGG TGGGCAAGCC TTCCTCTCAC 
AGTCAGTATA CCAGCAGTGG TTCTGTGTCT TCCTCAGGCA GCAAAAGCCA CCATAGCCAT 
TCTTCCTCCT CTTCCTCATC TGCTTCCACC TCAGGGAAGA TGAAAAGCAG TAAATCAGAA 
GGTTCATCAA GTTCCAAGTT AAGTAGCAGT ATGTATTCTA GCCAGGGGTC TTCTGGATCT 
AGCCAGTCCA AAAATTCATC CCAGTCTGGG GGGAAGCCAG GCTCCTCTCC CATAACCAAG 
CATGGACTGA GCAGTGGCTC TAGCAGCACC AAGATGAAAC CTCAAGGAAA GCCATCATCA 
CTTATGAATC CTTCTTTAAG TAAACCAAAC ATATCCCCTT CTCATTCAAG GCCACCTGGA 
GGCTCTGACA AGCTTGCCTC TCCAATGAAG CCTGTTCCTG GAACTCCTCC ATCCTCTAAA 
GCCAAGTCCC CTATCAGTTC AGGTTCTGGT GGTTCTCATA TGTCTGGAAC TAGTTCAAGC 
TCTGGCATGA AGTCATCTTC AGGGTTAGGA TCCTCAGGCT CGTTGTCCCA GAAAACTCCC 
CCATCATCTA ATTCCTGTAC GGCATCTTCC TCCTCCTTTT CCTCAAGTGG CTCTTCCATG 
TCATCCTCTC AGAACCAGCA TGGGAGTTCT AAAGGAAAAT CTCCCAGCAG AAACAAGAAG 
CCGTCCTTGA CAGCTGTCAT AGATAAACTG AAGCATGGGG TTGTCACCAG TGGCCCTGGG 
GGTGAAGACC CACTGGACGG CCAGATGGGG GTGAGCACAA ATTCTTCCAG CCATCCTATG 
TCCTCCAAAC ATAACATGTC AGGAGGAGAG TTTCAGGGCA AGCGTGAGAA AAGTGATAAA 
GACAAATCAA AGGTTTCCAC CTCCGGGAGT TCAGTGGATT CTTCTAAGAA GACCTCAGAG 
TCAAAAAATG TGGGGAGCAC AGGTGTGGCA AAAATTATCA TCAGTAAGCA TGATGGAGGC 
TCCCCTAGCA TTAAAGCCAA AGTGACTTTG CAGAAACCTG GGGAAAGTAG TGGAGAAGGG 
CTTAGGCCTC AAATGGCTTC TTCTAAAAAC TATGGCTCTC CACTCATCAG TGGTTCCACT 
CCAAAGCATG AGCGTGGCTC TCCCAGCCAT AGTAAGTCAC CAGCATATAC CCCCCAGAAT 
CTGGACAGTG AAAGTGAGTC AGGCTCCTCC ATAGCAGAGA AATCTTATCA GAATAGTCCC 
AGCTCAGACG ATGGTATCCG ACCACTTCCA GAATACAGCA CAGAGAAACA TAAGAAGCAC 
AAAAAGGAAA AGAAGAAAGT AAAAGACAAA GATAGGGACC GAGACCGGGA CAAAGACCGA 
GACAAGAAAA AATCTCATAG CATCAAGCCA GAGAGTTGGT CCAAATCACC CATCTCTTCA 
GACCAGTCCT TGTCTATGAC AAGTAACACA ATCTTATCTG CAGACAGACC CTCAAGGCTC 
AGCCCAGACT TTATGATTGG GGAGGAAGAT GATGATCTTA TGGATGTGGC CCTGATTGGG 
AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004765.2
CDS	213..4958
Translation	MKAQGETEESEKLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNAGPLDKILHGSVGYLTPRSGGHLMNLKYYVSPSDLLDDKTASPIILHENNVSRSLGMNASVTIEGTSAVYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYAPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEICSGSNKTKKKKSSRLPPEKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPAIGTPLRDSSSSGHSQSTLFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFHDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNNQADTVDFSIISVAGKALAPADLMEHHSGSQGPLLTTGDLGKEKTQKRVKEGNGTSNSTLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSAGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSASTSGKMKSSKSEGSSSSKLSSSMYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTSSSSGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPLDGQMGVSTNSSSHPMSSKHNMSGGEFQGKREKSDKDKSKVSTSGSSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSADRPSRLSPDFMIGEEDDDLMDVALIGN

Target ORF information:

RefSeq Version	NM_004774.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 1 (MED1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004774.3

ORF Insert Sequence:

ATGAAAGCTC AGGGGGAAAC CGAGGAGTCA GAAAAGCTGA GTAAGATGAG TTCTCTCCTG 
GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCCTGGA GTGAAACCAT TAAGCTTGTG 
CGTCAAGTCA TGGAGAAGAG GGTTGTGATG AGTTCTGGAG GGCATCAACA TTTGGTCAGC 
TGTTTGGAGA CATTGCAGAA GGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGATCGT 
TTGGAGTCCA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA 
TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT 
TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCGGA GCTTGTACAG 
CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGCCT TGTTAATCTG 
TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA 
GAACAAGATC TTTCTAAAAT GGCAATTATG TACTGGAAAG CAACTAATGC TGGTCCCTTG 
GATAAGATTC TTCATGGAAG TGTTGGCTAT CTCACACCAA GGAGTGGGGG TCATTTAATG 
AACCTGAAGT ACTATGTCTC TCCTTCTGAC CTACTGGATG ACAAGACTGC ATCTCCCATC 
ATTTTGCATG AGAATAATGT TTCTCGATCT TTGGGCATGA ATGCATCAGT GACAATTGAA 
GGAACATCTG CTGTGTACAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT 
GACAATAAAT GGACCCCTTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT 
GCCTGTTTCT TCTTGAAATT TCCCCAGCCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA 
CTGCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGC ACCCCTGTAT 
GAACTGATCA CTCAGTTTGA GCTATCAAAG GACCCTGACC CCATACCTTT GAATCACAAC 
ATGAGATTTT ATGCTGCTCT TCCTGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT 
CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTTGTTA GCAAAATCAC CTTTCAGCAC 
CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACACC AAGTGGCCTA TAACACCCTC 
ATTGGAAGCT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT TCTCCAATTT 
GAAGTGTGTC CTCTCTCAGA GTCTCGTTTC AGCGTATCTT TTCAGCACCC TGTGAATGAC 
TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTCTAC 
AAAGGGCTGT CGGATGCACT GATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA 
TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAAGCC 
GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG 
CCCCCGGCTA GCAGCCCAGG GTATGGCATG ACCACAGGCA ACAACCCAAT GAGTGGTACC 
ACTACACCAA CCAACACCTT TCCGGGGGGT CCCATTACCA CCTTGTTTAA TATGAGCATG 
AGCATCAAAG ATCGGCATGA GTCGGTGGGC CATGGGGAGG ACTTCAGCAA GGTGTCTCAG 
AACCCAATTC TTACCAGTTT GTTGCAAATC ACAGGGAACG GGGGGTCTAC CATTGGCTCG 
AGTCCGACCC CTCCTCATCA CACGCCGCCA CCTGTCTCTT CGATGGCCGG CAACACCAAG 
AACCACCCGA TGCTCATGAA CCTTCTTAAA GATAATCCTG CCCAGGATTT CTCAACCCTT 
TATGGAAGCA GCCCTTTAGA AAGGCAGAAC TCCTCTTCCG GCTCACCCCG CATGGAAATA 
TGCTCGGGGA GCAACAAGAC CAAGAAAAAG AAGTCATCAA GATTACCACC TGAGAAACCA 
AAGCACCAGA CTGAAGATGA CTTTCAGAGG GAGCTATTTT CAATGGATGT TGACTCACAG 
AACCCTATCT TTGATGTCAA CATGACAGCT GACACGCTGG ATACGCCACA CATCACTCCA 
GCTCCAAGCC AGTGTAGCAC TCCCCCAACA ACTTACCCAC AACCAGTACC TCACCCCCAA 
CCCAGTATTC AAAGGATGGT CCGACTATCC AGTTCAGACA GCATTGGCCC AGATGTAACT 
GACATCCTTT CAGACATTGC AGAAGAAGCT TCTAAACTTC CCAGCACTAG TGATGATTGC 
CCAGCCATTG GCACCCCTCT TCGAGATTCT TCAAGCTCTG GGCATTCTCA GAGTACCCTG 
TTTGACTCTG ATGTCTTTCA AACTAACAAT AATGAAAATC CATACACTGA TCCAGCTGAT 
CTTATTGCAG ATGCTGCTGG AAGCCCCAGT AGTGACTCTC CTACCAATCA TTTTTTTCAT 
GATGGAGTAG ATTTCAATCC TGATTTATTG AACAGCCAGA GCCAAAGTGG TTTTGGAGAA 
GAATATTTTG ATGAAAGCAG CCAAAGTGGG GATAATGATG ATTTCAAAGG ATTTGCATCT 
CAGGCACTAA ATACTTTGGG GGTGCCAATG CTTGGAGGTG ATAATGGGGA GACCAAGTTT 
AAGGGCAATA ACCAAGCCGA CACAGTTGAT TTCAGTATTA TTTCAGTAGC CGGCAAAGCT 
TTAGCTCCTG CAGATCTTAT GGAGCATCAC AGTGGTAGTC AGGGTCCTTT ACTGACCACT 
GGGGACTTAG GGAAAGAAAA GACTCAAAAG AGGGTAAAGG AAGGCAATGG CACCAGTAAT 
AGTACTCTCT CGGGGCCCGG ATTAGACAGC AAACCAGGGA AGCGCAGTCG GACCCCTTCT 
AATGATGGGA AAAGCAAAGA TAAGCCTCCA AAGCGGAAGA AGGCAGACAC TGAGGGAAAG 
TCTCCATCTC ATAGTTCTTC TAACAGACCT TTTACCCCAC CTACCAGTAC AGGTGGATCT 
AAATCGCCAG GCAGTGCAGG AAGATCTCAG ACTCCCCCAG GTGTTGCCAC ACCACCCATT 
CCCAAAATCA CTATTCAGAT TCCTAAGGGA ACAGTGATGG TGGGCAAGCC TTCCTCTCAC 
AGTCAGTATA CCAGCAGTGG TTCTGTGTCT TCCTCAGGCA GCAAAAGCCA CCATAGCCAT 
TCTTCCTCCT CTTCCTCATC TGCTTCCACC TCAGGGAAGA TGAAAAGCAG TAAATCAGAA 
GGTTCATCAA GTTCCAAGTT AAGTAGCAGT ATGTATTCTA GCCAGGGGTC TTCTGGATCT 
AGCCAGTCCA AAAATTCATC CCAGTCTGGG GGGAAGCCAG GCTCCTCTCC CATAACCAAG 
CATGGACTGA GCAGTGGCTC TAGCAGCACC AAGATGAAAC CTCAAGGAAA GCCATCATCA 
CTTATGAATC CTTCTTTAAG TAAACCAAAC ATATCCCCTT CTCATTCAAG GCCACCTGGA 
GGCTCTGACA AGCTTGCCTC TCCAATGAAG CCTGTTCCTG GAACTCCTCC ATCCTCTAAA 
GCCAAGTCCC CTATCAGTTC AGGTTCTGGT GGTTCTCATA TGTCTGGAAC TAGTTCAAGC 
TCTGGCATGA AGTCATCTTC AGGGTTAGGA TCCTCAGGCT CGTTGTCCCA GAAAACTCCC 
CCATCATCTA ATTCCTGTAC GGCATCTTCC TCCTCCTTTT CCTCAAGTGG CTCTTCCATG 
TCATCCTCTC AGAACCAGCA TGGGAGTTCT AAAGGAAAAT CTCCCAGCAG AAACAAGAAG 
CCGTCCTTGA CAGCTGTCAT AGATAAACTG AAGCATGGGG TTGTCACCAG TGGCCCTGGG 
GGTGAAGACC CACTGGACGG CCAGATGGGG GTGAGCACAA ATTCTTCCAG CCATCCTATG 
TCCTCCAAAC ATAACATGTC AGGAGGAGAG TTTCAGGGCA AGCGTGAGAA AAGTGATAAA 
GACAAATCAA AGGTTTCCAC CTCCGGGAGT TCAGTGGATT CTTCTAAGAA GACCTCAGAG 
TCAAAAAATG TGGGGAGCAC AGGTGTGGCA AAAATTATCA TCAGTAAGCA TGATGGAGGC 
TCCCCTAGCA TTAAAGCCAA AGTGACTTTG CAGAAACCTG GGGAAAGTAG TGGAGAAGGG 
CTTAGGCCTC AAATGGCTTC TTCTAAAAAC TATGGCTCTC CACTCATCAG TGGTTCCACT 
CCAAAGCATG AGCGTGGCTC TCCCAGCCAT AGTAAGTCAC CAGCATATAC CCCCCAGAAT 
CTGGACAGTG AAAGTGAGTC AGGCTCCTCC ATAGCAGAGA AATCTTATCA GAATAGTCCC 
AGCTCAGACG ATGGTATCCG ACCACTTCCA GAATACAGCA CAGAGAAACA TAAGAAGCAC 
AAAAAGGAAA AGAAGAAAGT AAAAGACAAA GATAGGGACC GAGACCGGGA CAAAGACCGA 
GACAAGAAAA AATCTCATAG CATCAAGCCA GAGAGTTGGT CCAAATCACC CATCTCTTCA 
GACCAGTCCT TGTCTATGAC AAGTAACACA ATCTTATCTG CAGACAGACC CTCAAGGCTC 
AGCCCAGACT TTATGATTGG GGAGGAAGAT GATGATCTTA TGGATGTGGC CCTGATTGGG 
AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED1 ( XM_006721957.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006722020.1 Homo sapiens mediator complex subunit 1 (MED1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu50485
Clone ID Related Accession (Same CDS sequence)	XM_006721957.1 , XM_006721957.2
Accession Version	XM_006721957.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCTC AGGGGGAAAC CGAGGAGTCA GAAAAGCTGA GTAAGATGAG TTCTCTCCTG 
GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCCTGGA GTGAAACCAT TAAGCTTGTG 
CGTCAAGTCA TGGAGAAGAG GGTTGTGATG AGTTCTGGAG GGCATCAACA TTTGGTCAGC 
TGTTTGGAGA CATTGCAGAA GGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGATCGT 
TTGGAGTCCA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA 
TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT 
TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCGGA GCTTGTACAG 
CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGCCT TGTTAATCTG 
TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA 
GAACAAGATC TTTCTAAAAT GGCAATTATG TACTGGAAAG CAACTAATGC TGGTCCCTTG 
GATAAGATTC TTCATGGAAG TGTTGGCTAT CTCACACCAA GGAGTGGGGG TCATTTAATG 
AACCTGAAGT ACTATGTCTC TCCTTCTGAC CTACTGGATG ACAAGACTGC ATCTCCCATC 
ATTTTGCATG AGAATAATGT TTCTCGATCT TTGGGCATGA ATGCATCAGT GACAATTGAA 
GGAACATCTG CTGTGTACAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT 
GACAATAAAT GGACCCCTTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT 
GCCTGTTTCT TCTTGAAATT TCCCCAGCCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA 
CTGCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGC ACCCCTGTAT 
GAACTGATCA CTCAGTTTGA GCTATCAAAG GACCCTGACC CCATACCTTT GAATCACAAC 
ATGAGATTTT ATGCTGCTCT TCCTGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT 
CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTTGTTA GCAAAATCAC CTTTCAGCAC 
CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACACC AAGTGGCCTA TAACACCCTC 
ATTGGAAGCT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT TCTCCAATTT 
GAAGTGTGTC CTCTCTCAGA GTCTCGTTTC AGCGTATCTT TTCAGCACCC TGTGAATGAC 
TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTCTAC 
AAAGGGCTGT CGGATGCACT GATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA 
TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAAGCC 
GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG 
CCCCCGGCTA GCAGCCCAGG GGAGCCGGGG CTAAACTGCT TCACCCTGCC TGAGAACCAG 
GGAGCACTGC ATTTCTCCAC AGGGTGGAGG AGAAGAGGCA GAATAAACCA AGCCTGGGAC 
ACCTCCCTCC TGTCTAGGTG TACTCATTCT TCTGTTTCAA AAGAAGGCAA GGACATGAAG 
TCAACTTCTA CCTATCTTCT GCTGCTGGTG TCTTATGTAT TCTCAGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006722020.1
CDS	210..2060
Translation	MKAQGETEESEKLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNAGPLDKILHGSVGYLTPRSGGHLMNLKYYVSPSDLLDDKTASPIILHENNVSRSLGMNASVTIEGTSAVYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYAPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGEPGLNCFTLPENQGALHFSTGWRRRGRINQAWDTSLLSRCTHSSVSKEGKDMKSTSTYLLLLVSYVFSV

Target ORF information:

RefSeq Version	XM_006721957.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 1 (MED1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006721957.1

ORF Insert Sequence:

ATGAAAGCTC AGGGGGAAAC CGAGGAGTCA GAAAAGCTGA GTAAGATGAG TTCTCTCCTG 
GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCCTGGA GTGAAACCAT TAAGCTTGTG 
CGTCAAGTCA TGGAGAAGAG GGTTGTGATG AGTTCTGGAG GGCATCAACA TTTGGTCAGC 
TGTTTGGAGA CATTGCAGAA GGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGATCGT 
TTGGAGTCCA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA 
TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT 
TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCGGA GCTTGTACAG 
CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGCCT TGTTAATCTG 
TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA 
GAACAAGATC TTTCTAAAAT GGCAATTATG TACTGGAAAG CAACTAATGC TGGTCCCTTG 
GATAAGATTC TTCATGGAAG TGTTGGCTAT CTCACACCAA GGAGTGGGGG TCATTTAATG 
AACCTGAAGT ACTATGTCTC TCCTTCTGAC CTACTGGATG ACAAGACTGC ATCTCCCATC 
ATTTTGCATG AGAATAATGT TTCTCGATCT TTGGGCATGA ATGCATCAGT GACAATTGAA 
GGAACATCTG CTGTGTACAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT 
GACAATAAAT GGACCCCTTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT 
GCCTGTTTCT TCTTGAAATT TCCCCAGCCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA 
CTGCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGC ACCCCTGTAT 
GAACTGATCA CTCAGTTTGA GCTATCAAAG GACCCTGACC CCATACCTTT GAATCACAAC 
ATGAGATTTT ATGCTGCTCT TCCTGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT 
CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTTGTTA GCAAAATCAC CTTTCAGCAC 
CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACACC AAGTGGCCTA TAACACCCTC 
ATTGGAAGCT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT TCTCCAATTT 
GAAGTGTGTC CTCTCTCAGA GTCTCGTTTC AGCGTATCTT TTCAGCACCC TGTGAATGAC 
TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTCTAC 
AAAGGGCTGT CGGATGCACT GATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA 
TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAAGCC 
GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG 
CCCCCGGCTA GCAGCCCAGG GGAGCCGGGG CTAAACTGCT TCACCCTGCC TGAGAACCAG 
GGAGCACTGC ATTTCTCCAC AGGGTGGAGG AGAAGAGGCA GAATAAACCA AGCCTGGGAC 
ACCTCCCTCC TGTCTAGGTG TACTCATTCT TCTGTTTCAA AAGAAGGCAA GGACATGAAG 
TCAACTTCTA CCTATCTTCT GCTGCTGGTG TCTTATGTAT TCTCAGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED1 ( XM_006721957.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006722020.1 Homo sapiens mediator complex subunit 1 (MED1), transcript variant X3, mRNA.

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CloneID	OHu50485
Clone ID Related Accession (Same CDS sequence)	XM_006721957.1 , XM_006721957.2
Accession Version	XM_006721957.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006721957.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCTC AGGGGGAAAC CGAGGAGTCA GAAAAGCTGA GTAAGATGAG TTCTCTCCTG 
GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCCTGGA GTGAAACCAT TAAGCTTGTG 
CGTCAAGTCA TGGAGAAGAG GGTTGTGATG AGTTCTGGAG GGCATCAACA TTTGGTCAGC 
TGTTTGGAGA CATTGCAGAA GGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGATCGT 
TTGGAGTCCA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA 
TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT 
TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCGGA GCTTGTACAG 
CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGCCT TGTTAATCTG 
TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA 
GAACAAGATC TTTCTAAAAT GGCAATTATG TACTGGAAAG CAACTAATGC TGGTCCCTTG 
GATAAGATTC TTCATGGAAG TGTTGGCTAT CTCACACCAA GGAGTGGGGG TCATTTAATG 
AACCTGAAGT ACTATGTCTC TCCTTCTGAC CTACTGGATG ACAAGACTGC ATCTCCCATC 
ATTTTGCATG AGAATAATGT TTCTCGATCT TTGGGCATGA ATGCATCAGT GACAATTGAA 
GGAACATCTG CTGTGTACAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT 
GACAATAAAT GGACCCCTTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT 
GCCTGTTTCT TCTTGAAATT TCCCCAGCCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA 
CTGCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGC ACCCCTGTAT 
GAACTGATCA CTCAGTTTGA GCTATCAAAG GACCCTGACC CCATACCTTT GAATCACAAC 
ATGAGATTTT ATGCTGCTCT TCCTGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT 
CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTTGTTA GCAAAATCAC CTTTCAGCAC 
CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACACC AAGTGGCCTA TAACACCCTC 
ATTGGAAGCT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT TCTCCAATTT 
GAAGTGTGTC CTCTCTCAGA GTCTCGTTTC AGCGTATCTT TTCAGCACCC TGTGAATGAC 
TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTCTAC 
AAAGGGCTGT CGGATGCACT GATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA 
TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAAGCC 
GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG 
CCCCCGGCTA GCAGCCCAGG GGAGCCGGGG CTAAACTGCT TCACCCTGCC TGAGAACCAG 
GGAGCACTGC ATTTCTCCAC AGGGTGGAGG AGAAGAGGCA GAATAAACCA AGCCTGGGAC 
ACCTCCCTCC TGTCTAGGTG TACTCATTCT TCTGTTTCAA AAGAAGGCAA GGACATGAAG 
TCAACTTCTA CCTATCTTCT GCTGCTGGTG TCTTATGTAT TCTCAGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006722020.1
CDS	213..2063
Translation	MKAQGETEESEKLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNAGPLDKILHGSVGYLTPRSGGHLMNLKYYVSPSDLLDDKTASPIILHENNVSRSLGMNASVTIEGTSAVYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYAPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGEPGLNCFTLPENQGALHFSTGWRRRGRINQAWDTSLLSRCTHSSVSKEGKDMKSTSTYLLLLVSYVFSV

Target ORF information:

RefSeq Version	XM_006721957.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 1 (MED1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006721957.2

ORF Insert Sequence:

ATGAAAGCTC AGGGGGAAAC CGAGGAGTCA GAAAAGCTGA GTAAGATGAG TTCTCTCCTG 
GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCCTGGA GTGAAACCAT TAAGCTTGTG 
CGTCAAGTCA TGGAGAAGAG GGTTGTGATG AGTTCTGGAG GGCATCAACA TTTGGTCAGC 
TGTTTGGAGA CATTGCAGAA GGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGATCGT 
TTGGAGTCCA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA 
TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT 
TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCGGA GCTTGTACAG 
CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGCCT TGTTAATCTG 
TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA 
GAACAAGATC TTTCTAAAAT GGCAATTATG TACTGGAAAG CAACTAATGC TGGTCCCTTG 
GATAAGATTC TTCATGGAAG TGTTGGCTAT CTCACACCAA GGAGTGGGGG TCATTTAATG 
AACCTGAAGT ACTATGTCTC TCCTTCTGAC CTACTGGATG ACAAGACTGC ATCTCCCATC 
ATTTTGCATG AGAATAATGT TTCTCGATCT TTGGGCATGA ATGCATCAGT GACAATTGAA 
GGAACATCTG CTGTGTACAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT 
GACAATAAAT GGACCCCTTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT 
GCCTGTTTCT TCTTGAAATT TCCCCAGCCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA 
CTGCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGC ACCCCTGTAT 
GAACTGATCA CTCAGTTTGA GCTATCAAAG GACCCTGACC CCATACCTTT GAATCACAAC 
ATGAGATTTT ATGCTGCTCT TCCTGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT 
CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTTGTTA GCAAAATCAC CTTTCAGCAC 
CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACACC AAGTGGCCTA TAACACCCTC 
ATTGGAAGCT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT TCTCCAATTT 
GAAGTGTGTC CTCTCTCAGA GTCTCGTTTC AGCGTATCTT TTCAGCACCC TGTGAATGAC 
TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTCTAC 
AAAGGGCTGT CGGATGCACT GATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA 
TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAAGCC 
GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG 
CCCCCGGCTA GCAGCCCAGG GGAGCCGGGG CTAAACTGCT TCACCCTGCC TGAGAACCAG 
GGAGCACTGC ATTTCTCCAC AGGGTGGAGG AGAAGAGGCA GAATAAACCA AGCCTGGGAC 
ACCTCCCTCC TGTCTAGGTG TACTCATTCT TCTGTTTCAA AAGAAGGCAA GGACATGAAG 
TCAACTTCTA CCTATCTTCT GCTGCTGGTG TCTTATGTAT TCTCAGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED1 ( XM_017024779.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880268.1 Homo sapiens mediator complex subunit 1 (MED1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu86938
Clone ID Related Accession (Same CDS sequence)	XM_017024779.1 , XM_017024779.2
Accession Version	XM_017024779.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTGA AGTACTATGT CTCTCCTTCT GACCTACTGG ATGACAAGAC TGCATCTCCC 
ATCATTTTGC ATGAGAATAA TGTTTCTCGA TCTTTGGGCA TGAATGCATC AGTGACAATT 
GAAGGAACAT CTGCTGTGTA CAAACTCCCA ATTGCACCAT TAATTATGGG GTCACATCCA 
GTTGACAATA AATGGACCCC TTCCTTCTCC TCAATCACCA GTGCCAACAG TGTTGATCTT 
CCTGCCTGTT TCTTCTTGAA ATTTCCCCAG CCAATCCCAG TATCTAGAGC ATTTGTTCAG 
AAACTGCAGA ACTGCACAGG AATTCCATTG TTTGAAACTC AACCAACTTA TGCACCCCTG 
TATGAACTGA TCACTCAGTT TGAGCTATCA AAGGACCCTG ACCCCATACC TTTGAATCAC 
AACATGAGAT TTTATGCTGC TCTTCCTGGT CAGCAGCACT GCTATTTCCT CAACAAGGAT 
GCTCCTCTTC CAGATGGCCG AAGTCTACAG GGAACCCTTG TTAGCAAAAT CACCTTTCAG 
CACCCTGGCC GAGTTCCTCT TATCCTAAAT CTGATCAGAC ACCAAGTGGC CTATAACACC 
CTCATTGGAA GCTGTGTCAA AAGAACTATT CTGAAAGAAG ATTCTCCTGG GCTTCTCCAA 
TTTGAAGTGT GTCCTCTCTC AGAGTCTCGT TTCAGCGTAT CTTTTCAGCA CCCTGTGAAT 
GACTCCCTGG TGTGTGTGGT AATGGATGTG CAGGACTCAA CACATGTGAG CTGTAAACTC 
TACAAAGGGC TGTCGGATGC ACTGATCTGC ACAGATGACT TCATTGCCAA AGTTGTTCAA 
AGATGTATGT CCATCCCTGT GACGATGAGG GCTATTCGGA GGAAAGCTGA AACCATTCAA 
GCCGACACCC CAGCACTGTC CCTCATTGCA GAGACAGTTG AAGACATGGT GAAAAAGAAC 
CTGCCCCCGG CTAGCAGCCC AGGGTATGGC ATGACCACAG GCAACAACCC AATGAGTGGT 
ACCACTACAC CAACCAACAC CTTTCCGGGG GGTCCCATTA CCACCTTGTT TAATATGAGC 
ATGAGCATCA AAGATCGGCA TGAGTCGGTG GGCCATGGGG AGGACTTCAG CAAGGTGTCT 
CAGAACCCAA TTCTTACCAG TTTGTTGCAA ATCACAGGGA ACGGGGGGTC TACCATTGGC 
TCGAGTCCGA CCCCTCCTCA TCACACGCCG CCACCTGTCT CTTCGATGGC CGGCAACACC 
AAGAACCACC CGATGCTCAT GAACCTTCTT AAAGATAATC CTGCCCAGGA TTTCTCAACC 
CTTTATGGAA GCAGCCCTTT AGAAAGGCAG AACTCCTCTT CCGGCTCACC CCGCATGGAA 
ATATGCTCGG GGAGCAACAA GACCAAGAAA AAGAAGTCAT CAAGATTACC ACCTGAGAAA 
CCAAAGCACC AGACTGAAGA TGACTTTCAG AGGGAGCTAT TTTCAATGGA TGTTGACTCA 
CAGAACCCTA TCTTTGATGT CAACATGACA GCTGACACGC TGGATACGCC ACACATCACT 
CCAGCTCCAA GCCAGTGTAG CACTCCCCCA ACAACTTACC CACAACCAGT ACCTCACCCC 
CAACCCAGTA TTCAAAGGAT GGTCCGACTA TCCAGTTCAG ACAGCATTGG CCCAGATGTA 
ACTGACATCC TTTCAGACAT TGCAGAAGAA GCTTCTAAAC TTCCCAGCAC TAGTGATGAT 
TGCCCAGCCA TTGGCACCCC TCTTCGAGAT TCTTCAAGCT CTGGGCATTC TCAGAGTACC 
CTGTTTGACT CTGATGTCTT TCAAACTAAC AATAATGAAA ATCCATACAC TGATCCAGCT 
GATCTTATTG CAGATGCTGC TGGAAGCCCC AGTAGTGACT CTCCTACCAA TCATTTTTTT 
CATGATGGAG TAGATTTCAA TCCTGATTTA TTGAACAGCC AGAGCCAAAG TGGTTTTGGA 
GAAGAATATT TTGATGAAAG CAGCCAAAGT GGGGATAATG ATGATTTCAA AGGATTTGCA 
TCTCAGGCAC TAAATACTTT GGGGGTGCCA ATGCTTGGAG GTGATAATGG GGAGACCAAG 
TTTAAGGGCA ATAACCAAGC CGACACAGTT GATTTCAGTA TTATTTCAGT AGCCGGCAAA 
GCTTTAGCTC CTGCAGATCT TATGGAGCAT CACAGTGGTA GTCAGGGTCC TTTACTGACC 
ACTGGGGACT TAGGGAAAGA AAAGACTCAA AAGAGGGTAA AGGAAGGCAA TGGCACCAGT 
AATAGTACTC TCTCGGGGCC CGGATTAGAC AGCAAACCAG GGAAGCGCAG TCGGACCCCT 
TCTAATGATG GGAAAAGCAA AGATAAGCCT CCAAAGCGGA AGAAGGCAGA CACTGAGGGA 
AAGTCTCCAT CTCATAGTTC TTCTAACAGA CCTTTTACCC CACCTACCAG TACAGGTGGA 
TCTAAATCGC CAGGCAGTGC AGGAAGATCT CAGACTCCCC CAGGTGTTGC CACACCACCC 
ATTCCCAAAA TCACTATTCA GATTCCTAAG GGAACAGTGA TGGTGGGCAA GCCTTCCTCT 
CACAGTCAGT ATACCAGCAG TGGTTCTGTG TCTTCCTCAG GCAGCAAAAG CCACCATAGC 
CATTCTTCCT CCTCTTCCTC ATCTGCTTCC ACCTCAGGGA AGATGAAAAG CAGTAAATCA 
GAAGGTTCAT CAAGTTCCAA GTTAAGTAGC AGTATGTATT CTAGCCAGGG GTCTTCTGGA 
TCTAGCCAGT CCAAAAATTC ATCCCAGTCT GGGGGGAAGC CAGGCTCCTC TCCCATAACC 
AAGCATGGAC TGAGCAGTGG CTCTAGCAGC ACCAAGATGA AACCTCAAGG AAAGCCATCA 
TCACTTATGA ATCCTTCTTT AAGTAAACCA AACATATCCC CTTCTCATTC AAGGCCACCT 
GGAGGCTCTG ACAAGCTTGC CTCTCCAATG AAGCCTGTTC CTGGAACTCC TCCATCCTCT 
AAAGCCAAGT CCCCTATCAG TTCAGGTTCT GGTGGTTCTC ATATGTCTGG AACTAGTTCA 
AGCTCTGGCA TGAAGTCATC TTCAGGGTTA GGATCCTCAG GCTCGTTGTC CCAGAAAACT 
CCCCCATCAT CTAATTCCTG TACGGCATCT TCCTCCTCCT TTTCCTCAAG TGGCTCTTCC 
ATGTCATCCT CTCAGAACCA GCATGGGAGT TCTAAAGGAA AATCTCCCAG CAGAAACAAG 
AAGCCGTCCT TGACAGCTGT CATAGATAAA CTGAAGCATG GGGTTGTCAC CAGTGGCCCT 
GGGGGTGAAG ACCCACTGGA CGGCCAGATG GGGGTGAGCA CAAATTCTTC CAGCCATCCT 
ATGTCCTCCA AACATAACAT GTCAGGAGGA GAGTTTCAGG GCAAGCGTGA GAAAAGTGAT 
AAAGACAAAT CAAAGGTTTC CACCTCCGGG AGTTCAGTGG ATTCTTCTAA GAAGACCTCA 
GAGTCAAAAA ATGTGGGGAG CACAGGTGTG GCAAAAATTA TCATCAGTAA GCATGATGGA 
GGCTCCCCTA GCATTAAAGC CAAAGTGACT TTGCAGAAAC CTGGGGAAAG TAGTGGAGAA 
GGGCTTAGGC CTCAAATGGC TTCTTCTAAA AACTATGGCT CTCCACTCAT CAGTGGTTCC 
ACTCCAAAGC ATGAGCGTGG CTCTCCCAGC CATAGTAAGT CACCAGCATA TACCCCCCAG 
AATCTGGACA GTGAAAGTGA GTCAGGCTCC TCCATAGCAG AGAAATCTTA TCAGAATAGT 
CCCAGCTCAG ACGATGGTAT CCGACCACTT CCAGAATACA GCACAGAGAA ACATAAGAAG 
CACAAAAAGG AAAAGAAGAA AGTAAAAGAC AAAGATAGGG ACCGAGACCG GGACAAAGAC 
CGAGACAAGA AAAAATCTCA TAGCATCAAG CCAGAGAGTT GGTCCAAATC ACCCATCTCT 
TCAGACCAGT CCTTGTCTAT GACAAGTAAC ACAATCTTAT CTGCAGACAG ACCCTCAAGG 
CTCAGCCCAG ACTTTATGAT TGGGGAGGAA GATGATGATC TTATGGATGT GGCCCTGATT 
GGGAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880268.1
CDS	803..4891
Translation	MNLKYYVSPSDLLDDKTASPIILHENNVSRSLGMNASVTIEGTSAVYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYAPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEICSGSNKTKKKKSSRLPPEKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPAIGTPLRDSSSSGHSQSTLFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFHDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNNQADTVDFSIISVAGKALAPADLMEHHSGSQGPLLTTGDLGKEKTQKRVKEGNGTSNSTLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSAGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSASTSGKMKSSKSEGSSSSKLSSSMYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTSSSSGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPLDGQMGVSTNSSSHPMSSKHNMSGGEFQGKREKSDKDKSKVSTSGSSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSADRPSRLSPDFMIGEEDDDLMDVALIGN

Target ORF information:

RefSeq Version	XM_017024779.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 1 (MED1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024779.1

ORF Insert Sequence:

ATGAACCTGA AGTACTATGT CTCTCCTTCT GACCTACTGG ATGACAAGAC TGCATCTCCC 
ATCATTTTGC ATGAGAATAA TGTTTCTCGA TCTTTGGGCA TGAATGCATC AGTGACAATT 
GAAGGAACAT CTGCTGTGTA CAAACTCCCA ATTGCACCAT TAATTATGGG GTCACATCCA 
GTTGACAATA AATGGACCCC TTCCTTCTCC TCAATCACCA GTGCCAACAG TGTTGATCTT 
CCTGCCTGTT TCTTCTTGAA ATTTCCCCAG CCAATCCCAG TATCTAGAGC ATTTGTTCAG 
AAACTGCAGA ACTGCACAGG AATTCCATTG TTTGAAACTC AACCAACTTA TGCACCCCTG 
TATGAACTGA TCACTCAGTT TGAGCTATCA AAGGACCCTG ACCCCATACC TTTGAATCAC 
AACATGAGAT TTTATGCTGC TCTTCCTGGT CAGCAGCACT GCTATTTCCT CAACAAGGAT 
GCTCCTCTTC CAGATGGCCG AAGTCTACAG GGAACCCTTG TTAGCAAAAT CACCTTTCAG 
CACCCTGGCC GAGTTCCTCT TATCCTAAAT CTGATCAGAC ACCAAGTGGC CTATAACACC 
CTCATTGGAA GCTGTGTCAA AAGAACTATT CTGAAAGAAG ATTCTCCTGG GCTTCTCCAA 
TTTGAAGTGT GTCCTCTCTC AGAGTCTCGT TTCAGCGTAT CTTTTCAGCA CCCTGTGAAT 
GACTCCCTGG TGTGTGTGGT AATGGATGTG CAGGACTCAA CACATGTGAG CTGTAAACTC 
TACAAAGGGC TGTCGGATGC ACTGATCTGC ACAGATGACT TCATTGCCAA AGTTGTTCAA 
AGATGTATGT CCATCCCTGT GACGATGAGG GCTATTCGGA GGAAAGCTGA AACCATTCAA 
GCCGACACCC CAGCACTGTC CCTCATTGCA GAGACAGTTG AAGACATGGT GAAAAAGAAC 
CTGCCCCCGG CTAGCAGCCC AGGGTATGGC ATGACCACAG GCAACAACCC AATGAGTGGT 
ACCACTACAC CAACCAACAC CTTTCCGGGG GGTCCCATTA CCACCTTGTT TAATATGAGC 
ATGAGCATCA AAGATCGGCA TGAGTCGGTG GGCCATGGGG AGGACTTCAG CAAGGTGTCT 
CAGAACCCAA TTCTTACCAG TTTGTTGCAA ATCACAGGGA ACGGGGGGTC TACCATTGGC 
TCGAGTCCGA CCCCTCCTCA TCACACGCCG CCACCTGTCT CTTCGATGGC CGGCAACACC 
AAGAACCACC CGATGCTCAT GAACCTTCTT AAAGATAATC CTGCCCAGGA TTTCTCAACC 
CTTTATGGAA GCAGCCCTTT AGAAAGGCAG AACTCCTCTT CCGGCTCACC CCGCATGGAA 
ATATGCTCGG GGAGCAACAA GACCAAGAAA AAGAAGTCAT CAAGATTACC ACCTGAGAAA 
CCAAAGCACC AGACTGAAGA TGACTTTCAG AGGGAGCTAT TTTCAATGGA TGTTGACTCA 
CAGAACCCTA TCTTTGATGT CAACATGACA GCTGACACGC TGGATACGCC ACACATCACT 
CCAGCTCCAA GCCAGTGTAG CACTCCCCCA ACAACTTACC CACAACCAGT ACCTCACCCC 
CAACCCAGTA TTCAAAGGAT GGTCCGACTA TCCAGTTCAG ACAGCATTGG CCCAGATGTA 
ACTGACATCC TTTCAGACAT TGCAGAAGAA GCTTCTAAAC TTCCCAGCAC TAGTGATGAT 
TGCCCAGCCA TTGGCACCCC TCTTCGAGAT TCTTCAAGCT CTGGGCATTC TCAGAGTACC 
CTGTTTGACT CTGATGTCTT TCAAACTAAC AATAATGAAA ATCCATACAC TGATCCAGCT 
GATCTTATTG CAGATGCTGC TGGAAGCCCC AGTAGTGACT CTCCTACCAA TCATTTTTTT 
CATGATGGAG TAGATTTCAA TCCTGATTTA TTGAACAGCC AGAGCCAAAG TGGTTTTGGA 
GAAGAATATT TTGATGAAAG CAGCCAAAGT GGGGATAATG ATGATTTCAA AGGATTTGCA 
TCTCAGGCAC TAAATACTTT GGGGGTGCCA ATGCTTGGAG GTGATAATGG GGAGACCAAG 
TTTAAGGGCA ATAACCAAGC CGACACAGTT GATTTCAGTA TTATTTCAGT AGCCGGCAAA 
GCTTTAGCTC CTGCAGATCT TATGGAGCAT CACAGTGGTA GTCAGGGTCC TTTACTGACC 
ACTGGGGACT TAGGGAAAGA AAAGACTCAA AAGAGGGTAA AGGAAGGCAA TGGCACCAGT 
AATAGTACTC TCTCGGGGCC CGGATTAGAC AGCAAACCAG GGAAGCGCAG TCGGACCCCT 
TCTAATGATG GGAAAAGCAA AGATAAGCCT CCAAAGCGGA AGAAGGCAGA CACTGAGGGA 
AAGTCTCCAT CTCATAGTTC TTCTAACAGA CCTTTTACCC CACCTACCAG TACAGGTGGA 
TCTAAATCGC CAGGCAGTGC AGGAAGATCT CAGACTCCCC CAGGTGTTGC CACACCACCC 
ATTCCCAAAA TCACTATTCA GATTCCTAAG GGAACAGTGA TGGTGGGCAA GCCTTCCTCT 
CACAGTCAGT ATACCAGCAG TGGTTCTGTG TCTTCCTCAG GCAGCAAAAG CCACCATAGC 
CATTCTTCCT CCTCTTCCTC ATCTGCTTCC ACCTCAGGGA AGATGAAAAG CAGTAAATCA 
GAAGGTTCAT CAAGTTCCAA GTTAAGTAGC AGTATGTATT CTAGCCAGGG GTCTTCTGGA 
TCTAGCCAGT CCAAAAATTC ATCCCAGTCT GGGGGGAAGC CAGGCTCCTC TCCCATAACC 
AAGCATGGAC TGAGCAGTGG CTCTAGCAGC ACCAAGATGA AACCTCAAGG AAAGCCATCA 
TCACTTATGA ATCCTTCTTT AAGTAAACCA AACATATCCC CTTCTCATTC AAGGCCACCT 
GGAGGCTCTG ACAAGCTTGC CTCTCCAATG AAGCCTGTTC CTGGAACTCC TCCATCCTCT 
AAAGCCAAGT CCCCTATCAG TTCAGGTTCT GGTGGTTCTC ATATGTCTGG AACTAGTTCA 
AGCTCTGGCA TGAAGTCATC TTCAGGGTTA GGATCCTCAG GCTCGTTGTC CCAGAAAACT 
CCCCCATCAT CTAATTCCTG TACGGCATCT TCCTCCTCCT TTTCCTCAAG TGGCTCTTCC 
ATGTCATCCT CTCAGAACCA GCATGGGAGT TCTAAAGGAA AATCTCCCAG CAGAAACAAG 
AAGCCGTCCT TGACAGCTGT CATAGATAAA CTGAAGCATG GGGTTGTCAC CAGTGGCCCT 
GGGGGTGAAG ACCCACTGGA CGGCCAGATG GGGGTGAGCA CAAATTCTTC CAGCCATCCT 
ATGTCCTCCA AACATAACAT GTCAGGAGGA GAGTTTCAGG GCAAGCGTGA GAAAAGTGAT 
AAAGACAAAT CAAAGGTTTC CACCTCCGGG AGTTCAGTGG ATTCTTCTAA GAAGACCTCA 
GAGTCAAAAA ATGTGGGGAG CACAGGTGTG GCAAAAATTA TCATCAGTAA GCATGATGGA 
GGCTCCCCTA GCATTAAAGC CAAAGTGACT TTGCAGAAAC CTGGGGAAAG TAGTGGAGAA 
GGGCTTAGGC CTCAAATGGC TTCTTCTAAA AACTATGGCT CTCCACTCAT CAGTGGTTCC 
ACTCCAAAGC ATGAGCGTGG CTCTCCCAGC CATAGTAAGT CACCAGCATA TACCCCCCAG 
AATCTGGACA GTGAAAGTGA GTCAGGCTCC TCCATAGCAG AGAAATCTTA TCAGAATAGT 
CCCAGCTCAG ACGATGGTAT CCGACCACTT CCAGAATACA GCACAGAGAA ACATAAGAAG 
CACAAAAAGG AAAAGAAGAA AGTAAAAGAC AAAGATAGGG ACCGAGACCG GGACAAAGAC 
CGAGACAAGA AAAAATCTCA TAGCATCAAG CCAGAGAGTT GGTCCAAATC ACCCATCTCT 
TCAGACCAGT CCTTGTCTAT GACAAGTAAC ACAATCTTAT CTGCAGACAG ACCCTCAAGG 
CTCAGCCCAG ACTTTATGAT TGGGGAGGAA GATGATGATC TTATGGATGT GGCCCTGATT 
GGGAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED1 ( XM_017024779.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880268.1 Homo sapiens mediator complex subunit 1 (MED1), transcript variant X2, mRNA.

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CloneID	OHu86938
Clone ID Related Accession (Same CDS sequence)	XM_017024779.1 , XM_017024779.2
Accession Version	XM_017024779.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017024779.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTGA AGTACTATGT CTCTCCTTCT GACCTACTGG ATGACAAGAC TGCATCTCCC 
ATCATTTTGC ATGAGAATAA TGTTTCTCGA TCTTTGGGCA TGAATGCATC AGTGACAATT 
GAAGGAACAT CTGCTGTGTA CAAACTCCCA ATTGCACCAT TAATTATGGG GTCACATCCA 
GTTGACAATA AATGGACCCC TTCCTTCTCC TCAATCACCA GTGCCAACAG TGTTGATCTT 
CCTGCCTGTT TCTTCTTGAA ATTTCCCCAG CCAATCCCAG TATCTAGAGC ATTTGTTCAG 
AAACTGCAGA ACTGCACAGG AATTCCATTG TTTGAAACTC AACCAACTTA TGCACCCCTG 
TATGAACTGA TCACTCAGTT TGAGCTATCA AAGGACCCTG ACCCCATACC TTTGAATCAC 
AACATGAGAT TTTATGCTGC TCTTCCTGGT CAGCAGCACT GCTATTTCCT CAACAAGGAT 
GCTCCTCTTC CAGATGGCCG AAGTCTACAG GGAACCCTTG TTAGCAAAAT CACCTTTCAG 
CACCCTGGCC GAGTTCCTCT TATCCTAAAT CTGATCAGAC ACCAAGTGGC CTATAACACC 
CTCATTGGAA GCTGTGTCAA AAGAACTATT CTGAAAGAAG ATTCTCCTGG GCTTCTCCAA 
TTTGAAGTGT GTCCTCTCTC AGAGTCTCGT TTCAGCGTAT CTTTTCAGCA CCCTGTGAAT 
GACTCCCTGG TGTGTGTGGT AATGGATGTG CAGGACTCAA CACATGTGAG CTGTAAACTC 
TACAAAGGGC TGTCGGATGC ACTGATCTGC ACAGATGACT TCATTGCCAA AGTTGTTCAA 
AGATGTATGT CCATCCCTGT GACGATGAGG GCTATTCGGA GGAAAGCTGA AACCATTCAA 
GCCGACACCC CAGCACTGTC CCTCATTGCA GAGACAGTTG AAGACATGGT GAAAAAGAAC 
CTGCCCCCGG CTAGCAGCCC AGGGTATGGC ATGACCACAG GCAACAACCC AATGAGTGGT 
ACCACTACAC CAACCAACAC CTTTCCGGGG GGTCCCATTA CCACCTTGTT TAATATGAGC 
ATGAGCATCA AAGATCGGCA TGAGTCGGTG GGCCATGGGG AGGACTTCAG CAAGGTGTCT 
CAGAACCCAA TTCTTACCAG TTTGTTGCAA ATCACAGGGA ACGGGGGGTC TACCATTGGC 
TCGAGTCCGA CCCCTCCTCA TCACACGCCG CCACCTGTCT CTTCGATGGC CGGCAACACC 
AAGAACCACC CGATGCTCAT GAACCTTCTT AAAGATAATC CTGCCCAGGA TTTCTCAACC 
CTTTATGGAA GCAGCCCTTT AGAAAGGCAG AACTCCTCTT CCGGCTCACC CCGCATGGAA 
ATATGCTCGG GGAGCAACAA GACCAAGAAA AAGAAGTCAT CAAGATTACC ACCTGAGAAA 
CCAAAGCACC AGACTGAAGA TGACTTTCAG AGGGAGCTAT TTTCAATGGA TGTTGACTCA 
CAGAACCCTA TCTTTGATGT CAACATGACA GCTGACACGC TGGATACGCC ACACATCACT 
CCAGCTCCAA GCCAGTGTAG CACTCCCCCA ACAACTTACC CACAACCAGT ACCTCACCCC 
CAACCCAGTA TTCAAAGGAT GGTCCGACTA TCCAGTTCAG ACAGCATTGG CCCAGATGTA 
ACTGACATCC TTTCAGACAT TGCAGAAGAA GCTTCTAAAC TTCCCAGCAC TAGTGATGAT 
TGCCCAGCCA TTGGCACCCC TCTTCGAGAT TCTTCAAGCT CTGGGCATTC TCAGAGTACC 
CTGTTTGACT CTGATGTCTT TCAAACTAAC AATAATGAAA ATCCATACAC TGATCCAGCT 
GATCTTATTG CAGATGCTGC TGGAAGCCCC AGTAGTGACT CTCCTACCAA TCATTTTTTT 
CATGATGGAG TAGATTTCAA TCCTGATTTA TTGAACAGCC AGAGCCAAAG TGGTTTTGGA 
GAAGAATATT TTGATGAAAG CAGCCAAAGT GGGGATAATG ATGATTTCAA AGGATTTGCA 
TCTCAGGCAC TAAATACTTT GGGGGTGCCA ATGCTTGGAG GTGATAATGG GGAGACCAAG 
TTTAAGGGCA ATAACCAAGC CGACACAGTT GATTTCAGTA TTATTTCAGT AGCCGGCAAA 
GCTTTAGCTC CTGCAGATCT TATGGAGCAT CACAGTGGTA GTCAGGGTCC TTTACTGACC 
ACTGGGGACT TAGGGAAAGA AAAGACTCAA AAGAGGGTAA AGGAAGGCAA TGGCACCAGT 
AATAGTACTC TCTCGGGGCC CGGATTAGAC AGCAAACCAG GGAAGCGCAG TCGGACCCCT 
TCTAATGATG GGAAAAGCAA AGATAAGCCT CCAAAGCGGA AGAAGGCAGA CACTGAGGGA 
AAGTCTCCAT CTCATAGTTC TTCTAACAGA CCTTTTACCC CACCTACCAG TACAGGTGGA 
TCTAAATCGC CAGGCAGTGC AGGAAGATCT CAGACTCCCC CAGGTGTTGC CACACCACCC 
ATTCCCAAAA TCACTATTCA GATTCCTAAG GGAACAGTGA TGGTGGGCAA GCCTTCCTCT 
CACAGTCAGT ATACCAGCAG TGGTTCTGTG TCTTCCTCAG GCAGCAAAAG CCACCATAGC 
CATTCTTCCT CCTCTTCCTC ATCTGCTTCC ACCTCAGGGA AGATGAAAAG CAGTAAATCA 
GAAGGTTCAT CAAGTTCCAA GTTAAGTAGC AGTATGTATT CTAGCCAGGG GTCTTCTGGA 
TCTAGCCAGT CCAAAAATTC ATCCCAGTCT GGGGGGAAGC CAGGCTCCTC TCCCATAACC 
AAGCATGGAC TGAGCAGTGG CTCTAGCAGC ACCAAGATGA AACCTCAAGG AAAGCCATCA 
TCACTTATGA ATCCTTCTTT AAGTAAACCA AACATATCCC CTTCTCATTC AAGGCCACCT 
GGAGGCTCTG ACAAGCTTGC CTCTCCAATG AAGCCTGTTC CTGGAACTCC TCCATCCTCT 
AAAGCCAAGT CCCCTATCAG TTCAGGTTCT GGTGGTTCTC ATATGTCTGG AACTAGTTCA 
AGCTCTGGCA TGAAGTCATC TTCAGGGTTA GGATCCTCAG GCTCGTTGTC CCAGAAAACT 
CCCCCATCAT CTAATTCCTG TACGGCATCT TCCTCCTCCT TTTCCTCAAG TGGCTCTTCC 
ATGTCATCCT CTCAGAACCA GCATGGGAGT TCTAAAGGAA AATCTCCCAG CAGAAACAAG 
AAGCCGTCCT TGACAGCTGT CATAGATAAA CTGAAGCATG GGGTTGTCAC CAGTGGCCCT 
GGGGGTGAAG ACCCACTGGA CGGCCAGATG GGGGTGAGCA CAAATTCTTC CAGCCATCCT 
ATGTCCTCCA AACATAACAT GTCAGGAGGA GAGTTTCAGG GCAAGCGTGA GAAAAGTGAT 
AAAGACAAAT CAAAGGTTTC CACCTCCGGG AGTTCAGTGG ATTCTTCTAA GAAGACCTCA 
GAGTCAAAAA ATGTGGGGAG CACAGGTGTG GCAAAAATTA TCATCAGTAA GCATGATGGA 
GGCTCCCCTA GCATTAAAGC CAAAGTGACT TTGCAGAAAC CTGGGGAAAG TAGTGGAGAA 
GGGCTTAGGC CTCAAATGGC TTCTTCTAAA AACTATGGCT CTCCACTCAT CAGTGGTTCC 
ACTCCAAAGC ATGAGCGTGG CTCTCCCAGC CATAGTAAGT CACCAGCATA TACCCCCCAG 
AATCTGGACA GTGAAAGTGA GTCAGGCTCC TCCATAGCAG AGAAATCTTA TCAGAATAGT 
CCCAGCTCAG ACGATGGTAT CCGACCACTT CCAGAATACA GCACAGAGAA ACATAAGAAG 
CACAAAAAGG AAAAGAAGAA AGTAAAAGAC AAAGATAGGG ACCGAGACCG GGACAAAGAC 
CGAGACAAGA AAAAATCTCA TAGCATCAAG CCAGAGAGTT GGTCCAAATC ACCCATCTCT 
TCAGACCAGT CCTTGTCTAT GACAAGTAAC ACAATCTTAT CTGCAGACAG ACCCTCAAGG 
CTCAGCCCAG ACTTTATGAT TGGGGAGGAA GATGATGATC TTATGGATGT GGCCCTGATT 
GGGAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880268.1
CDS	787..4875
Translation	MNLKYYVSPSDLLDDKTASPIILHENNVSRSLGMNASVTIEGTSAVYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYAPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEICSGSNKTKKKKSSRLPPEKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPAIGTPLRDSSSSGHSQSTLFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFHDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNNQADTVDFSIISVAGKALAPADLMEHHSGSQGPLLTTGDLGKEKTQKRVKEGNGTSNSTLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSAGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSASTSGKMKSSKSEGSSSSKLSSSMYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTSSSSGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPLDGQMGVSTNSSSHPMSSKHNMSGGEFQGKREKSDKDKSKVSTSGSSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSADRPSRLSPDFMIGEEDDDLMDVALIGN

Target ORF information:

RefSeq Version	XM_017024779.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 1 (MED1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024779.2

ORF Insert Sequence:

ATGAACCTGA AGTACTATGT CTCTCCTTCT GACCTACTGG ATGACAAGAC TGCATCTCCC 
ATCATTTTGC ATGAGAATAA TGTTTCTCGA TCTTTGGGCA TGAATGCATC AGTGACAATT 
GAAGGAACAT CTGCTGTGTA CAAACTCCCA ATTGCACCAT TAATTATGGG GTCACATCCA 
GTTGACAATA AATGGACCCC TTCCTTCTCC TCAATCACCA GTGCCAACAG TGTTGATCTT 
CCTGCCTGTT TCTTCTTGAA ATTTCCCCAG CCAATCCCAG TATCTAGAGC ATTTGTTCAG 
AAACTGCAGA ACTGCACAGG AATTCCATTG TTTGAAACTC AACCAACTTA TGCACCCCTG 
TATGAACTGA TCACTCAGTT TGAGCTATCA AAGGACCCTG ACCCCATACC TTTGAATCAC 
AACATGAGAT TTTATGCTGC TCTTCCTGGT CAGCAGCACT GCTATTTCCT CAACAAGGAT 
GCTCCTCTTC CAGATGGCCG AAGTCTACAG GGAACCCTTG TTAGCAAAAT CACCTTTCAG 
CACCCTGGCC GAGTTCCTCT TATCCTAAAT CTGATCAGAC ACCAAGTGGC CTATAACACC 
CTCATTGGAA GCTGTGTCAA AAGAACTATT CTGAAAGAAG ATTCTCCTGG GCTTCTCCAA 
TTTGAAGTGT GTCCTCTCTC AGAGTCTCGT TTCAGCGTAT CTTTTCAGCA CCCTGTGAAT 
GACTCCCTGG TGTGTGTGGT AATGGATGTG CAGGACTCAA CACATGTGAG CTGTAAACTC 
TACAAAGGGC TGTCGGATGC ACTGATCTGC ACAGATGACT TCATTGCCAA AGTTGTTCAA 
AGATGTATGT CCATCCCTGT GACGATGAGG GCTATTCGGA GGAAAGCTGA AACCATTCAA 
GCCGACACCC CAGCACTGTC CCTCATTGCA GAGACAGTTG AAGACATGGT GAAAAAGAAC 
CTGCCCCCGG CTAGCAGCCC AGGGTATGGC ATGACCACAG GCAACAACCC AATGAGTGGT 
ACCACTACAC CAACCAACAC CTTTCCGGGG GGTCCCATTA CCACCTTGTT TAATATGAGC 
ATGAGCATCA AAGATCGGCA TGAGTCGGTG GGCCATGGGG AGGACTTCAG CAAGGTGTCT 
CAGAACCCAA TTCTTACCAG TTTGTTGCAA ATCACAGGGA ACGGGGGGTC TACCATTGGC 
TCGAGTCCGA CCCCTCCTCA TCACACGCCG CCACCTGTCT CTTCGATGGC CGGCAACACC 
AAGAACCACC CGATGCTCAT GAACCTTCTT AAAGATAATC CTGCCCAGGA TTTCTCAACC 
CTTTATGGAA GCAGCCCTTT AGAAAGGCAG AACTCCTCTT CCGGCTCACC CCGCATGGAA 
ATATGCTCGG GGAGCAACAA GACCAAGAAA AAGAAGTCAT CAAGATTACC ACCTGAGAAA 
CCAAAGCACC AGACTGAAGA TGACTTTCAG AGGGAGCTAT TTTCAATGGA TGTTGACTCA 
CAGAACCCTA TCTTTGATGT CAACATGACA GCTGACACGC TGGATACGCC ACACATCACT 
CCAGCTCCAA GCCAGTGTAG CACTCCCCCA ACAACTTACC CACAACCAGT ACCTCACCCC 
CAACCCAGTA TTCAAAGGAT GGTCCGACTA TCCAGTTCAG ACAGCATTGG CCCAGATGTA 
ACTGACATCC TTTCAGACAT TGCAGAAGAA GCTTCTAAAC TTCCCAGCAC TAGTGATGAT 
TGCCCAGCCA TTGGCACCCC TCTTCGAGAT TCTTCAAGCT CTGGGCATTC TCAGAGTACC 
CTGTTTGACT CTGATGTCTT TCAAACTAAC AATAATGAAA ATCCATACAC TGATCCAGCT 
GATCTTATTG CAGATGCTGC TGGAAGCCCC AGTAGTGACT CTCCTACCAA TCATTTTTTT 
CATGATGGAG TAGATTTCAA TCCTGATTTA TTGAACAGCC AGAGCCAAAG TGGTTTTGGA 
GAAGAATATT TTGATGAAAG CAGCCAAAGT GGGGATAATG ATGATTTCAA AGGATTTGCA 
TCTCAGGCAC TAAATACTTT GGGGGTGCCA ATGCTTGGAG GTGATAATGG GGAGACCAAG 
TTTAAGGGCA ATAACCAAGC CGACACAGTT GATTTCAGTA TTATTTCAGT AGCCGGCAAA 
GCTTTAGCTC CTGCAGATCT TATGGAGCAT CACAGTGGTA GTCAGGGTCC TTTACTGACC 
ACTGGGGACT TAGGGAAAGA AAAGACTCAA AAGAGGGTAA AGGAAGGCAA TGGCACCAGT 
AATAGTACTC TCTCGGGGCC CGGATTAGAC AGCAAACCAG GGAAGCGCAG TCGGACCCCT 
TCTAATGATG GGAAAAGCAA AGATAAGCCT CCAAAGCGGA AGAAGGCAGA CACTGAGGGA 
AAGTCTCCAT CTCATAGTTC TTCTAACAGA CCTTTTACCC CACCTACCAG TACAGGTGGA 
TCTAAATCGC CAGGCAGTGC AGGAAGATCT CAGACTCCCC CAGGTGTTGC CACACCACCC 
ATTCCCAAAA TCACTATTCA GATTCCTAAG GGAACAGTGA TGGTGGGCAA GCCTTCCTCT 
CACAGTCAGT ATACCAGCAG TGGTTCTGTG TCTTCCTCAG GCAGCAAAAG CCACCATAGC 
CATTCTTCCT CCTCTTCCTC ATCTGCTTCC ACCTCAGGGA AGATGAAAAG CAGTAAATCA 
GAAGGTTCAT CAAGTTCCAA GTTAAGTAGC AGTATGTATT CTAGCCAGGG GTCTTCTGGA 
TCTAGCCAGT CCAAAAATTC ATCCCAGTCT GGGGGGAAGC CAGGCTCCTC TCCCATAACC 
AAGCATGGAC TGAGCAGTGG CTCTAGCAGC ACCAAGATGA AACCTCAAGG AAAGCCATCA 
TCACTTATGA ATCCTTCTTT AAGTAAACCA AACATATCCC CTTCTCATTC AAGGCCACCT 
GGAGGCTCTG ACAAGCTTGC CTCTCCAATG AAGCCTGTTC CTGGAACTCC TCCATCCTCT 
AAAGCCAAGT CCCCTATCAG TTCAGGTTCT GGTGGTTCTC ATATGTCTGG AACTAGTTCA 
AGCTCTGGCA TGAAGTCATC TTCAGGGTTA GGATCCTCAG GCTCGTTGTC CCAGAAAACT 
CCCCCATCAT CTAATTCCTG TACGGCATCT TCCTCCTCCT TTTCCTCAAG TGGCTCTTCC 
ATGTCATCCT CTCAGAACCA GCATGGGAGT TCTAAAGGAA AATCTCCCAG CAGAAACAAG 
AAGCCGTCCT TGACAGCTGT CATAGATAAA CTGAAGCATG GGGTTGTCAC CAGTGGCCCT 
GGGGGTGAAG ACCCACTGGA CGGCCAGATG GGGGTGAGCA CAAATTCTTC CAGCCATCCT 
ATGTCCTCCA AACATAACAT GTCAGGAGGA GAGTTTCAGG GCAAGCGTGA GAAAAGTGAT 
AAAGACAAAT CAAAGGTTTC CACCTCCGGG AGTTCAGTGG ATTCTTCTAA GAAGACCTCA 
GAGTCAAAAA ATGTGGGGAG CACAGGTGTG GCAAAAATTA TCATCAGTAA GCATGATGGA 
GGCTCCCCTA GCATTAAAGC CAAAGTGACT TTGCAGAAAC CTGGGGAAAG TAGTGGAGAA 
GGGCTTAGGC CTCAAATGGC TTCTTCTAAA AACTATGGCT CTCCACTCAT CAGTGGTTCC 
ACTCCAAAGC ATGAGCGTGG CTCTCCCAGC CATAGTAAGT CACCAGCATA TACCCCCCAG 
AATCTGGACA GTGAAAGTGA GTCAGGCTCC TCCATAGCAG AGAAATCTTA TCAGAATAGT 
CCCAGCTCAG ACGATGGTAT CCGACCACTT CCAGAATACA GCACAGAGAA ACATAAGAAG 
CACAAAAAGG AAAAGAAGAA AGTAAAAGAC AAAGATAGGG ACCGAGACCG GGACAAAGAC 
CGAGACAAGA AAAAATCTCA TAGCATCAAG CCAGAGAGTT GGTCCAAATC ACCCATCTCT 
TCAGACCAGT CCTTGTCTAT GACAAGTAAC ACAATCTTAT CTGCAGACAG ACCCTCAAGG 
CTCAGCCCAG ACTTTATGAT TGGGGAGGAA GATGATGATC TTATGGATGT GGCCCTGATT 
GGGAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED1 ( XM_005257465.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005257522.1 Homo sapiens mediator complex subunit 1 (MED1), transcript variant X1, mRNA.

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CloneID	OHu50484
Clone ID Related Accession (Same CDS sequence)	XM_005257465.3
Accession Version	XM_005257465.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257465.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTGG CTCTCCAATC CTTAGAACAA GATCTTTCTA AAATGGCAAT TATGTACTGG 
AAAGCAACTA ATGCTGGTCC CTTGGATAAG ATTCTTCATG GAAGTGTTGG CTATCTCACA 
CCAAGGAGTG GGGGTCATTT AATGAACCTG AAGTACTATG TCTCTCCTTC TGACCTACTG 
GATGACAAGA CTGCATCTCC CATCATTTTG CATGAGAATA ATGTTTCTCG ATCTTTGGGC 
ATGAATGCAT CAGTGACAAT TGAAGGAACA TCTGCTGTGT ACAAACTCCC AATTGCACCA 
TTAATTATGG GGTCACATCC AGTTGACAAT AAATGGACCC CTTCCTTCTC CTCAATCACC 
AGTGCCAACA GTGTTGATCT TCCTGCCTGT TTCTTCTTGA AATTTCCCCA GCCAATCCCA 
GTATCTAGAG CATTTGTTCA GAAACTGCAG AACTGCACAG GAATTCCATT GTTTGAAACT 
CAACCAACTT ATGCACCCCT GTATGAACTG ATCACTCAGT TTGAGCTATC AAAGGACCCT 
GACCCCATAC CTTTGAATCA CAACATGAGA TTTTATGCTG CTCTTCCTGG TCAGCAGCAC 
TGCTATTTCC TCAACAAGGA TGCTCCTCTT CCAGATGGCC GAAGTCTACA GGGAACCCTT 
GTTAGCAAAA TCACCTTTCA GCACCCTGGC CGAGTTCCTC TTATCCTAAA TCTGATCAGA 
CACCAAGTGG CCTATAACAC CCTCATTGGA AGCTGTGTCA AAAGAACTAT TCTGAAAGAA 
GATTCTCCTG GGCTTCTCCA ATTTGAAGTG TGTCCTCTCT CAGAGTCTCG TTTCAGCGTA 
TCTTTTCAGC ACCCTGTGAA TGACTCCCTG GTGTGTGTGG TAATGGATGT GCAGGACTCA 
ACACATGTGA GCTGTAAACT CTACAAAGGG CTGTCGGATG CACTGATCTG CACAGATGAC 
TTCATTGCCA AAGTTGTTCA AAGATGTATG TCCATCCCTG TGACGATGAG GGCTATTCGG 
AGGAAAGCTG AAACCATTCA AGCCGACACC CCAGCACTGT CCCTCATTGC AGAGACAGTT 
GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA 
GGCAACAACC CAATGAGTGG TACCACTACA CCAACCAACA CCTTTCCGGG GGGTCCCATT 
ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG 
GAGGACTTCA GCAAGGTGTC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG 
AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC 
TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAT 
CCTGCCCAGG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCCTCT 
TCCGGCTCAC CCCGCATGGA AATATGCTCG GGGAGCAACA AGACCAAGAA AAAGAAGTCA 
TCAAGATTAC CACCTGAGAA ACCAAAGCAC CAGACTGAAG ATGACTTTCA GAGGGAGCTA 
TTTTCAATGG ATGTTGACTC ACAGAACCCT ATCTTTGATG TCAACATGAC AGCTGACACG 
CTGGATACGC CACACATCAC TCCAGCTCCA AGCCAGTGTA GCACTCCCCC AACAACTTAC 
CCACAACCAG TACCTCACCC CCAACCCAGT ATTCAAAGGA TGGTCCGACT ATCCAGTTCA 
GACAGCATTG GCCCAGATGT AACTGACATC CTTTCAGACA TTGCAGAAGA AGCTTCTAAA 
CTTCCCAGCA CTAGTGATGA TTGCCCAGCC ATTGGCACCC CTCTTCGAGA TTCTTCAAGC 
TCTGGGCATT CTCAGAGTAC CCTGTTTGAC TCTGATGTCT TTCAAACTAA CAATAATGAA 
AATCCATACA CTGATCCAGC TGATCTTATT GCAGATGCTG CTGGAAGCCC CAGTAGTGAC 
TCTCCTACCA ATCATTTTTT TCATGATGGA GTAGATTTCA ATCCTGATTT ATTGAACAGC 
CAGAGCCAAA GTGGTTTTGG AGAAGAATAT TTTGATGAAA GCAGCCAAAG TGGGGATAAT 
GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGGTGCC AATGCTTGGA 
GGTGATAATG GGGAGACCAA GTTTAAGGGC AATAACCAAG CCGACACAGT TGATTTCAGT 
ATTATTTCAG TAGCCGGCAA AGCTTTAGCT CCTGCAGATC TTATGGAGCA TCACAGTGGT 
AGTCAGGGTC CTTTACTGAC CACTGGGGAC TTAGGGAAAG AAAAGACTCA AAAGAGGGTA 
AAGGAAGGCA ATGGCACCAG TAATAGTACT CTCTCGGGGC CCGGATTAGA CAGCAAACCA 
GGGAAGCGCA GTCGGACCCC TTCTAATGAT GGGAAAAGCA AAGATAAGCC TCCAAAGCGG 
AAGAAGGCAG ACACTGAGGG AAAGTCTCCA TCTCATAGTT CTTCTAACAG ACCTTTTACC 
CCACCTACCA GTACAGGTGG ATCTAAATCG CCAGGCAGTG CAGGAAGATC TCAGACTCCC 
CCAGGTGTTG CCACACCACC CATTCCCAAA ATCACTATTC AGATTCCTAA GGGAACAGTG 
ATGGTGGGCA AGCCTTCCTC TCACAGTCAG TATACCAGCA GTGGTTCTGT GTCTTCCTCA 
GGCAGCAAAA GCCACCATAG CCATTCTTCC TCCTCTTCCT CATCTGCTTC CACCTCAGGG 
AAGATGAAAA GCAGTAAATC AGAAGGTTCA TCAAGTTCCA AGTTAAGTAG CAGTATGTAT 
TCTAGCCAGG GGTCTTCTGG ATCTAGCCAG TCCAAAAATT CATCCCAGTC TGGGGGGAAG 
CCAGGCTCCT CTCCCATAAC CAAGCATGGA CTGAGCAGTG GCTCTAGCAG CACCAAGATG 
AAACCTCAAG GAAAGCCATC ATCACTTATG AATCCTTCTT TAAGTAAACC AAACATATCC 
CCTTCTCATT CAAGGCCACC TGGAGGCTCT GACAAGCTTG CCTCTCCAAT GAAGCCTGTT 
CCTGGAACTC CTCCATCCTC TAAAGCCAAG TCCCCTATCA GTTCAGGTTC TGGTGGTTCT 
CATATGTCTG GAACTAGTTC AAGCTCTGGC ATGAAGTCAT CTTCAGGGTT AGGATCCTCA 
GGCTCGTTGT CCCAGAAAAC TCCCCCATCA TCTAATTCCT GTACGGCATC TTCCTCCTCC 
TTTTCCTCAA GTGGCTCTTC CATGTCATCC TCTCAGAACC AGCATGGGAG TTCTAAAGGA 
AAATCTCCCA GCAGAAACAA GAAGCCGTCC TTGACAGCTG TCATAGATAA ACTGAAGCAT 
GGGGTTGTCA CCAGTGGCCC TGGGGGTGAA GACCCACTGG ACGGCCAGAT GGGGGTGAGC 
ACAAATTCTT CCAGCCATCC TATGTCCTCC AAACATAACA TGTCAGGAGG AGAGTTTCAG 
GGCAAGCGTG AGAAAAGTGA TAAAGACAAA TCAAAGGTTT CCACCTCCGG GAGTTCAGTG 
GATTCTTCTA AGAAGACCTC AGAGTCAAAA AATGTGGGGA GCACAGGTGT GGCAAAAATT 
ATCATCAGTA AGCATGATGG AGGCTCCCCT AGCATTAAAG CCAAAGTGAC TTTGCAGAAA 
CCTGGGGAAA GTAGTGGAGA AGGGCTTAGG CCTCAAATGG CTTCTTCTAA AAACTATGGC 
TCTCCACTCA TCAGTGGTTC CACTCCAAAG CATGAGCGTG GCTCTCCCAG CCATAGTAAG 
TCACCAGCAT ATACCCCCCA GAATCTGGAC AGTGAAAGTG AGTCAGGCTC CTCCATAGCA 
GAGAAATCTT ATCAGAATAG TCCCAGCTCA GACGATGGTA TCCGACCACT TCCAGAATAC 
AGCACAGAGA AACATAAGAA GCACAAAAAG GAAAAGAAGA AAGTAAAAGA CAAAGATAGG 
GACCGAGACC GGGACAAAGA CCGAGACAAG AAAAAATCTC ATAGCATCAA GCCAGAGAGT 
TGGTCCAAAT CACCCATCTC TTCAGACCAG TCCTTGTCTA TGACAAGTAA CACAATCTTA 
TCTGCAGACA GACCCTCAAG GCTCAGCCCA GACTTTATGA TTGGGGAGGA AGATGATGAT 
CTTATGGATG TGGCCCTGAT TGGGAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005257522.1
CDS	603..4832
Translation	MYLALQSLEQDLSKMAIMYWKATNAGPLDKILHGSVGYLTPRSGGHLMNLKYYVSPSDLLDDKTASPIILHENNVSRSLGMNASVTIEGTSAVYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYAPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEICSGSNKTKKKKSSRLPPEKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPAIGTPLRDSSSSGHSQSTLFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFHDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNNQADTVDFSIISVAGKALAPADLMEHHSGSQGPLLTTGDLGKEKTQKRVKEGNGTSNSTLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSAGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSASTSGKMKSSKSEGSSSSKLSSSMYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTSSSSGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPLDGQMGVSTNSSSHPMSSKHNMSGGEFQGKREKSDKDKSKVSTSGSSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSADRPSRLSPDFMIGEEDDDLMDVALIGN

Target ORF information:

RefSeq Version	XM_005257465.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 1 (MED1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005257465.3

ORF Insert Sequence:

ATGTACTTGG CTCTCCAATC CTTAGAACAA GATCTTTCTA AAATGGCAAT TATGTACTGG 
AAAGCAACTA ATGCTGGTCC CTTGGATAAG ATTCTTCATG GAAGTGTTGG CTATCTCACA 
CCAAGGAGTG GGGGTCATTT AATGAACCTG AAGTACTATG TCTCTCCTTC TGACCTACTG 
GATGACAAGA CTGCATCTCC CATCATTTTG CATGAGAATA ATGTTTCTCG ATCTTTGGGC 
ATGAATGCAT CAGTGACAAT TGAAGGAACA TCTGCTGTGT ACAAACTCCC AATTGCACCA 
TTAATTATGG GGTCACATCC AGTTGACAAT AAATGGACCC CTTCCTTCTC CTCAATCACC 
AGTGCCAACA GTGTTGATCT TCCTGCCTGT TTCTTCTTGA AATTTCCCCA GCCAATCCCA 
GTATCTAGAG CATTTGTTCA GAAACTGCAG AACTGCACAG GAATTCCATT GTTTGAAACT 
CAACCAACTT ATGCACCCCT GTATGAACTG ATCACTCAGT TTGAGCTATC AAAGGACCCT 
GACCCCATAC CTTTGAATCA CAACATGAGA TTTTATGCTG CTCTTCCTGG TCAGCAGCAC 
TGCTATTTCC TCAACAAGGA TGCTCCTCTT CCAGATGGCC GAAGTCTACA GGGAACCCTT 
GTTAGCAAAA TCACCTTTCA GCACCCTGGC CGAGTTCCTC TTATCCTAAA TCTGATCAGA 
CACCAAGTGG CCTATAACAC CCTCATTGGA AGCTGTGTCA AAAGAACTAT TCTGAAAGAA 
GATTCTCCTG GGCTTCTCCA ATTTGAAGTG TGTCCTCTCT CAGAGTCTCG TTTCAGCGTA 
TCTTTTCAGC ACCCTGTGAA TGACTCCCTG GTGTGTGTGG TAATGGATGT GCAGGACTCA 
ACACATGTGA GCTGTAAACT CTACAAAGGG CTGTCGGATG CACTGATCTG CACAGATGAC 
TTCATTGCCA AAGTTGTTCA AAGATGTATG TCCATCCCTG TGACGATGAG GGCTATTCGG 
AGGAAAGCTG AAACCATTCA AGCCGACACC CCAGCACTGT CCCTCATTGC AGAGACAGTT 
GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA 
GGCAACAACC CAATGAGTGG TACCACTACA CCAACCAACA CCTTTCCGGG GGGTCCCATT 
ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG 
GAGGACTTCA GCAAGGTGTC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG 
AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC 
TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAT 
CCTGCCCAGG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCCTCT 
TCCGGCTCAC CCCGCATGGA AATATGCTCG GGGAGCAACA AGACCAAGAA AAAGAAGTCA 
TCAAGATTAC CACCTGAGAA ACCAAAGCAC CAGACTGAAG ATGACTTTCA GAGGGAGCTA 
TTTTCAATGG ATGTTGACTC ACAGAACCCT ATCTTTGATG TCAACATGAC AGCTGACACG 
CTGGATACGC CACACATCAC TCCAGCTCCA AGCCAGTGTA GCACTCCCCC AACAACTTAC 
CCACAACCAG TACCTCACCC CCAACCCAGT ATTCAAAGGA TGGTCCGACT ATCCAGTTCA 
GACAGCATTG GCCCAGATGT AACTGACATC CTTTCAGACA TTGCAGAAGA AGCTTCTAAA 
CTTCCCAGCA CTAGTGATGA TTGCCCAGCC ATTGGCACCC CTCTTCGAGA TTCTTCAAGC 
TCTGGGCATT CTCAGAGTAC CCTGTTTGAC TCTGATGTCT TTCAAACTAA CAATAATGAA 
AATCCATACA CTGATCCAGC TGATCTTATT GCAGATGCTG CTGGAAGCCC CAGTAGTGAC 
TCTCCTACCA ATCATTTTTT TCATGATGGA GTAGATTTCA ATCCTGATTT ATTGAACAGC 
CAGAGCCAAA GTGGTTTTGG AGAAGAATAT TTTGATGAAA GCAGCCAAAG TGGGGATAAT 
GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGGTGCC AATGCTTGGA 
GGTGATAATG GGGAGACCAA GTTTAAGGGC AATAACCAAG CCGACACAGT TGATTTCAGT 
ATTATTTCAG TAGCCGGCAA AGCTTTAGCT CCTGCAGATC TTATGGAGCA TCACAGTGGT 
AGTCAGGGTC CTTTACTGAC CACTGGGGAC TTAGGGAAAG AAAAGACTCA AAAGAGGGTA 
AAGGAAGGCA ATGGCACCAG TAATAGTACT CTCTCGGGGC CCGGATTAGA CAGCAAACCA 
GGGAAGCGCA GTCGGACCCC TTCTAATGAT GGGAAAAGCA AAGATAAGCC TCCAAAGCGG 
AAGAAGGCAG ACACTGAGGG AAAGTCTCCA TCTCATAGTT CTTCTAACAG ACCTTTTACC 
CCACCTACCA GTACAGGTGG ATCTAAATCG CCAGGCAGTG CAGGAAGATC TCAGACTCCC 
CCAGGTGTTG CCACACCACC CATTCCCAAA ATCACTATTC AGATTCCTAA GGGAACAGTG 
ATGGTGGGCA AGCCTTCCTC TCACAGTCAG TATACCAGCA GTGGTTCTGT GTCTTCCTCA 
GGCAGCAAAA GCCACCATAG CCATTCTTCC TCCTCTTCCT CATCTGCTTC CACCTCAGGG 
AAGATGAAAA GCAGTAAATC AGAAGGTTCA TCAAGTTCCA AGTTAAGTAG CAGTATGTAT 
TCTAGCCAGG GGTCTTCTGG ATCTAGCCAG TCCAAAAATT CATCCCAGTC TGGGGGGAAG 
CCAGGCTCCT CTCCCATAAC CAAGCATGGA CTGAGCAGTG GCTCTAGCAG CACCAAGATG 
AAACCTCAAG GAAAGCCATC ATCACTTATG AATCCTTCTT TAAGTAAACC AAACATATCC 
CCTTCTCATT CAAGGCCACC TGGAGGCTCT GACAAGCTTG CCTCTCCAAT GAAGCCTGTT 
CCTGGAACTC CTCCATCCTC TAAAGCCAAG TCCCCTATCA GTTCAGGTTC TGGTGGTTCT 
CATATGTCTG GAACTAGTTC AAGCTCTGGC ATGAAGTCAT CTTCAGGGTT AGGATCCTCA 
GGCTCGTTGT CCCAGAAAAC TCCCCCATCA TCTAATTCCT GTACGGCATC TTCCTCCTCC 
TTTTCCTCAA GTGGCTCTTC CATGTCATCC TCTCAGAACC AGCATGGGAG TTCTAAAGGA 
AAATCTCCCA GCAGAAACAA GAAGCCGTCC TTGACAGCTG TCATAGATAA ACTGAAGCAT 
GGGGTTGTCA CCAGTGGCCC TGGGGGTGAA GACCCACTGG ACGGCCAGAT GGGGGTGAGC 
ACAAATTCTT CCAGCCATCC TATGTCCTCC AAACATAACA TGTCAGGAGG AGAGTTTCAG 
GGCAAGCGTG AGAAAAGTGA TAAAGACAAA TCAAAGGTTT CCACCTCCGG GAGTTCAGTG 
GATTCTTCTA AGAAGACCTC AGAGTCAAAA AATGTGGGGA GCACAGGTGT GGCAAAAATT 
ATCATCAGTA AGCATGATGG AGGCTCCCCT AGCATTAAAG CCAAAGTGAC TTTGCAGAAA 
CCTGGGGAAA GTAGTGGAGA AGGGCTTAGG CCTCAAATGG CTTCTTCTAA AAACTATGGC 
TCTCCACTCA TCAGTGGTTC CACTCCAAAG CATGAGCGTG GCTCTCCCAG CCATAGTAAG 
TCACCAGCAT ATACCCCCCA GAATCTGGAC AGTGAAAGTG AGTCAGGCTC CTCCATAGCA 
GAGAAATCTT ATCAGAATAG TCCCAGCTCA GACGATGGTA TCCGACCACT TCCAGAATAC 
AGCACAGAGA AACATAAGAA GCACAAAAAG GAAAAGAAGA AAGTAAAAGA CAAAGATAGG 
GACCGAGACC GGGACAAAGA CCGAGACAAG AAAAAATCTC ATAGCATCAA GCCAGAGAGT 
TGGTCCAAAT CACCCATCTC TTCAGACCAG TCCTTGTCTA TGACAAGTAA CACAATCTTA 
TCTGCAGACA GACCCTCAAG GCTCAGCCCA GACTTTATGA TTGGGGAGGA AGATGATGAT 
CTTATGGATG TGGCCCTGAT TGGGAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HER2-Driven Breast Tumorigenesis Relies upon Interactions of the Estrogen Receptor with Coactivator MED1.
Cancer research78(2)422-435(2018 Jan)
Yang Y,Leonard M,Zhang Y,Zhao D,Mahmoud C,Khan S,Wang J,Lower EE,Zhang X

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

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MED1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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