MED1 cDNA ORF clone, Homo sapiens(human)

The following MED1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu17285 NM_004774.4
Latest version!
Homo sapiens mediator complex subunit 1 (MED1), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$579.50-$811.30
$1159.00
OHu50485 XM_006721957.2
Latest version!
Homo sapiens mediator complex subunit 1 (MED1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu86938 XM_017024779.2
Latest version!
Homo sapiens mediator complex subunit 1 (MED1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $727.30
$1039.00
OHu50484 XM_005257465.3
Latest version!
Homo sapiens mediator complex subunit 1 (MED1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $727.30
$1039.00
View Old Accession Versions >>
OHu86938 XM_017024779.1 Homo sapiens mediator complex subunit 1 (MED1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $727.30
$1039.00
OHu17285 NM_004774.3 Homo sapiens mediator complex subunit 1 (MED1), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$579.50-$811.30
$1159.00
OHu50485 XM_006721957.1 Homo sapiens mediator complex subunit 1 (MED1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu17285
    Clone ID Related Accession (Same CDS sequence) NM_004774.4 , NM_004774.3
    Accession Version NM_004774.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4746bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Homo sapiens(human)
    Product mediator of RNA polymerase II transcription subunit 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP357374.1, BC131783.1 and AC005288.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_004774.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y13467.1, BC131783.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000300651.11/ ENSP00000300651.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAAAGCTC AGGGGGAAAC CGAGGAGTCA GAAAAGCTGA GTAAGATGAG TTCTCTCCTG 
    GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCCTGGA GTGAAACCAT TAAGCTTGTG
    CGTCAAGTCA TGGAGAAGAG GGTTGTGATG AGTTCTGGAG GGCATCAACA TTTGGTCAGC
    TGTTTGGAGA CATTGCAGAA GGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGATCGT
    TTGGAGTCCA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA
    TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT
    TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCGGA GCTTGTACAG
    CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGCCT TGTTAATCTG
    TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA
    GAACAAGATC TTTCTAAAAT GGCAATTATG TACTGGAAAG CAACTAATGC TGGTCCCTTG
    GATAAGATTC TTCATGGAAG TGTTGGCTAT CTCACACCAA GGAGTGGGGG TCATTTAATG
    AACCTGAAGT ACTATGTCTC TCCTTCTGAC CTACTGGATG ACAAGACTGC ATCTCCCATC
    ATTTTGCATG AGAATAATGT TTCTCGATCT TTGGGCATGA ATGCATCAGT GACAATTGAA
    GGAACATCTG CTGTGTACAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT
    GACAATAAAT GGACCCCTTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT
    GCCTGTTTCT TCTTGAAATT TCCCCAGCCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA
    CTGCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGC ACCCCTGTAT
    GAACTGATCA CTCAGTTTGA GCTATCAAAG GACCCTGACC CCATACCTTT GAATCACAAC
    ATGAGATTTT ATGCTGCTCT TCCTGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT
    CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTTGTTA GCAAAATCAC CTTTCAGCAC
    CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACACC AAGTGGCCTA TAACACCCTC
    ATTGGAAGCT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT TCTCCAATTT
    GAAGTGTGTC CTCTCTCAGA GTCTCGTTTC AGCGTATCTT TTCAGCACCC TGTGAATGAC
    TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTCTAC
    AAAGGGCTGT CGGATGCACT GATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA
    TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAAGCC
    GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG
    CCCCCGGCTA GCAGCCCAGG GTATGGCATG ACCACAGGCA ACAACCCAAT GAGTGGTACC
    ACTACACCAA CCAACACCTT TCCGGGGGGT CCCATTACCA CCTTGTTTAA TATGAGCATG
    AGCATCAAAG ATCGGCATGA GTCGGTGGGC CATGGGGAGG ACTTCAGCAA GGTGTCTCAG
    AACCCAATTC TTACCAGTTT GTTGCAAATC ACAGGGAACG GGGGGTCTAC CATTGGCTCG
    AGTCCGACCC CTCCTCATCA CACGCCGCCA CCTGTCTCTT CGATGGCCGG CAACACCAAG
    AACCACCCGA TGCTCATGAA CCTTCTTAAA GATAATCCTG CCCAGGATTT CTCAACCCTT
    TATGGAAGCA GCCCTTTAGA AAGGCAGAAC TCCTCTTCCG GCTCACCCCG CATGGAAATA
    TGCTCGGGGA GCAACAAGAC CAAGAAAAAG AAGTCATCAA GATTACCACC TGAGAAACCA
    AAGCACCAGA CTGAAGATGA CTTTCAGAGG GAGCTATTTT CAATGGATGT TGACTCACAG
    AACCCTATCT TTGATGTCAA CATGACAGCT GACACGCTGG ATACGCCACA CATCACTCCA
    GCTCCAAGCC AGTGTAGCAC TCCCCCAACA ACTTACCCAC AACCAGTACC TCACCCCCAA
    CCCAGTATTC AAAGGATGGT CCGACTATCC AGTTCAGACA GCATTGGCCC AGATGTAACT
    GACATCCTTT CAGACATTGC AGAAGAAGCT TCTAAACTTC CCAGCACTAG TGATGATTGC
    CCAGCCATTG GCACCCCTCT TCGAGATTCT TCAAGCTCTG GGCATTCTCA GAGTACCCTG
    TTTGACTCTG ATGTCTTTCA AACTAACAAT AATGAAAATC CATACACTGA TCCAGCTGAT
    CTTATTGCAG ATGCTGCTGG AAGCCCCAGT AGTGACTCTC CTACCAATCA TTTTTTTCAT
    GATGGAGTAG ATTTCAATCC TGATTTATTG AACAGCCAGA GCCAAAGTGG TTTTGGAGAA
    GAATATTTTG ATGAAAGCAG CCAAAGTGGG GATAATGATG ATTTCAAAGG ATTTGCATCT
    CAGGCACTAA ATACTTTGGG GGTGCCAATG CTTGGAGGTG ATAATGGGGA GACCAAGTTT
    AAGGGCAATA ACCAAGCCGA CACAGTTGAT TTCAGTATTA TTTCAGTAGC CGGCAAAGCT
    TTAGCTCCTG CAGATCTTAT GGAGCATCAC AGTGGTAGTC AGGGTCCTTT ACTGACCACT
    GGGGACTTAG GGAAAGAAAA GACTCAAAAG AGGGTAAAGG AAGGCAATGG CACCAGTAAT
    AGTACTCTCT CGGGGCCCGG ATTAGACAGC AAACCAGGGA AGCGCAGTCG GACCCCTTCT
    AATGATGGGA AAAGCAAAGA TAAGCCTCCA AAGCGGAAGA AGGCAGACAC TGAGGGAAAG
    TCTCCATCTC ATAGTTCTTC TAACAGACCT TTTACCCCAC CTACCAGTAC AGGTGGATCT
    AAATCGCCAG GCAGTGCAGG AAGATCTCAG ACTCCCCCAG GTGTTGCCAC ACCACCCATT
    CCCAAAATCA CTATTCAGAT TCCTAAGGGA ACAGTGATGG TGGGCAAGCC TTCCTCTCAC
    AGTCAGTATA CCAGCAGTGG TTCTGTGTCT TCCTCAGGCA GCAAAAGCCA CCATAGCCAT
    TCTTCCTCCT CTTCCTCATC TGCTTCCACC TCAGGGAAGA TGAAAAGCAG TAAATCAGAA
    GGTTCATCAA GTTCCAAGTT AAGTAGCAGT ATGTATTCTA GCCAGGGGTC TTCTGGATCT
    AGCCAGTCCA AAAATTCATC CCAGTCTGGG GGGAAGCCAG GCTCCTCTCC CATAACCAAG
    CATGGACTGA GCAGTGGCTC TAGCAGCACC AAGATGAAAC CTCAAGGAAA GCCATCATCA
    CTTATGAATC CTTCTTTAAG TAAACCAAAC ATATCCCCTT CTCATTCAAG GCCACCTGGA
    GGCTCTGACA AGCTTGCCTC TCCAATGAAG CCTGTTCCTG GAACTCCTCC ATCCTCTAAA
    GCCAAGTCCC CTATCAGTTC AGGTTCTGGT GGTTCTCATA TGTCTGGAAC TAGTTCAAGC
    TCTGGCATGA AGTCATCTTC AGGGTTAGGA TCCTCAGGCT CGTTGTCCCA GAAAACTCCC
    CCATCATCTA ATTCCTGTAC GGCATCTTCC TCCTCCTTTT CCTCAAGTGG CTCTTCCATG
    TCATCCTCTC AGAACCAGCA TGGGAGTTCT AAAGGAAAAT CTCCCAGCAG AAACAAGAAG
    CCGTCCTTGA CAGCTGTCAT AGATAAACTG AAGCATGGGG TTGTCACCAG TGGCCCTGGG
    GGTGAAGACC CACTGGACGG CCAGATGGGG GTGAGCACAA ATTCTTCCAG CCATCCTATG
    TCCTCCAAAC ATAACATGTC AGGAGGAGAG TTTCAGGGCA AGCGTGAGAA AAGTGATAAA
    GACAAATCAA AGGTTTCCAC CTCCGGGAGT TCAGTGGATT CTTCTAAGAA GACCTCAGAG
    TCAAAAAATG TGGGGAGCAC AGGTGTGGCA AAAATTATCA TCAGTAAGCA TGATGGAGGC
    TCCCCTAGCA TTAAAGCCAA AGTGACTTTG CAGAAACCTG GGGAAAGTAG TGGAGAAGGG
    CTTAGGCCTC AAATGGCTTC TTCTAAAAAC TATGGCTCTC CACTCATCAG TGGTTCCACT
    CCAAAGCATG AGCGTGGCTC TCCCAGCCAT AGTAAGTCAC CAGCATATAC CCCCCAGAAT
    CTGGACAGTG AAAGTGAGTC AGGCTCCTCC ATAGCAGAGA AATCTTATCA GAATAGTCCC
    AGCTCAGACG ATGGTATCCG ACCACTTCCA GAATACAGCA CAGAGAAACA TAAGAAGCAC
    AAAAAGGAAA AGAAGAAAGT AAAAGACAAA GATAGGGACC GAGACCGGGA CAAAGACCGA
    GACAAGAAAA AATCTCATAG CATCAAGCCA GAGAGTTGGT CCAAATCACC CATCTCTTCA
    GACCAGTCCT TGTCTATGAC AAGTAACACA ATCTTATCTG CAGACAGACC CTCAAGGCTC
    AGCCCAGACT TTATGATTGG GGAGGAAGAT GATGATCTTA TGGATGTGGC CCTGATTGGG
    AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004765.2
    CDS202..4947
    Translation

    Target ORF information:

    RefSeq Version NM_004774.4
    Organism Homo sapiens(human)
    Definition Homo sapiens mediator complex subunit 1 (MED1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004774.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAAAGCTC AGGGGGAAAC CGAGGAGTCA GAAAAGCTGA GTAAGATGAG TTCTCTCCTG 
    GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCCTGGA GTGAAACCAT TAAGCTTGTG
    CGTCAAGTCA TGGAGAAGAG GGTTGTGATG AGTTCTGGAG GGCATCAACA TTTGGTCAGC
    TGTTTGGAGA CATTGCAGAA GGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGATCGT
    TTGGAGTCCA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA
    TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT
    TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCGGA GCTTGTACAG
    CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGCCT TGTTAATCTG
    TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA
    GAACAAGATC TTTCTAAAAT GGCAATTATG TACTGGAAAG CAACTAATGC TGGTCCCTTG
    GATAAGATTC TTCATGGAAG TGTTGGCTAT CTCACACCAA GGAGTGGGGG TCATTTAATG
    AACCTGAAGT ACTATGTCTC TCCTTCTGAC CTACTGGATG ACAAGACTGC ATCTCCCATC
    ATTTTGCATG AGAATAATGT TTCTCGATCT TTGGGCATGA ATGCATCAGT GACAATTGAA
    GGAACATCTG CTGTGTACAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT
    GACAATAAAT GGACCCCTTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT
    GCCTGTTTCT TCTTGAAATT TCCCCAGCCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA
    CTGCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGC ACCCCTGTAT
    GAACTGATCA CTCAGTTTGA GCTATCAAAG GACCCTGACC CCATACCTTT GAATCACAAC
    ATGAGATTTT ATGCTGCTCT TCCTGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT
    CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTTGTTA GCAAAATCAC CTTTCAGCAC
    CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACACC AAGTGGCCTA TAACACCCTC
    ATTGGAAGCT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT TCTCCAATTT
    GAAGTGTGTC CTCTCTCAGA GTCTCGTTTC AGCGTATCTT TTCAGCACCC TGTGAATGAC
    TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTCTAC
    AAAGGGCTGT CGGATGCACT GATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA
    TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAAGCC
    GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG
    CCCCCGGCTA GCAGCCCAGG GTATGGCATG ACCACAGGCA ACAACCCAAT GAGTGGTACC
    ACTACACCAA CCAACACCTT TCCGGGGGGT CCCATTACCA CCTTGTTTAA TATGAGCATG
    AGCATCAAAG ATCGGCATGA GTCGGTGGGC CATGGGGAGG ACTTCAGCAA GGTGTCTCAG
    AACCCAATTC TTACCAGTTT GTTGCAAATC ACAGGGAACG GGGGGTCTAC CATTGGCTCG
    AGTCCGACCC CTCCTCATCA CACGCCGCCA CCTGTCTCTT CGATGGCCGG CAACACCAAG
    AACCACCCGA TGCTCATGAA CCTTCTTAAA GATAATCCTG CCCAGGATTT CTCAACCCTT
    TATGGAAGCA GCCCTTTAGA AAGGCAGAAC TCCTCTTCCG GCTCACCCCG CATGGAAATA
    TGCTCGGGGA GCAACAAGAC CAAGAAAAAG AAGTCATCAA GATTACCACC TGAGAAACCA
    AAGCACCAGA CTGAAGATGA CTTTCAGAGG GAGCTATTTT CAATGGATGT TGACTCACAG
    AACCCTATCT TTGATGTCAA CATGACAGCT GACACGCTGG ATACGCCACA CATCACTCCA
    GCTCCAAGCC AGTGTAGCAC TCCCCCAACA ACTTACCCAC AACCAGTACC TCACCCCCAA
    CCCAGTATTC AAAGGATGGT CCGACTATCC AGTTCAGACA GCATTGGCCC AGATGTAACT
    GACATCCTTT CAGACATTGC AGAAGAAGCT TCTAAACTTC CCAGCACTAG TGATGATTGC
    CCAGCCATTG GCACCCCTCT TCGAGATTCT TCAAGCTCTG GGCATTCTCA GAGTACCCTG
    TTTGACTCTG ATGTCTTTCA AACTAACAAT AATGAAAATC CATACACTGA TCCAGCTGAT
    CTTATTGCAG ATGCTGCTGG AAGCCCCAGT AGTGACTCTC CTACCAATCA TTTTTTTCAT
    GATGGAGTAG ATTTCAATCC TGATTTATTG AACAGCCAGA GCCAAAGTGG TTTTGGAGAA
    GAATATTTTG ATGAAAGCAG CCAAAGTGGG GATAATGATG ATTTCAAAGG ATTTGCATCT
    CAGGCACTAA ATACTTTGGG GGTGCCAATG CTTGGAGGTG ATAATGGGGA GACCAAGTTT
    AAGGGCAATA ACCAAGCCGA CACAGTTGAT TTCAGTATTA TTTCAGTAGC CGGCAAAGCT
    TTAGCTCCTG CAGATCTTAT GGAGCATCAC AGTGGTAGTC AGGGTCCTTT ACTGACCACT
    GGGGACTTAG GGAAAGAAAA GACTCAAAAG AGGGTAAAGG AAGGCAATGG CACCAGTAAT
    AGTACTCTCT CGGGGCCCGG ATTAGACAGC AAACCAGGGA AGCGCAGTCG GACCCCTTCT
    AATGATGGGA AAAGCAAAGA TAAGCCTCCA AAGCGGAAGA AGGCAGACAC TGAGGGAAAG
    TCTCCATCTC ATAGTTCTTC TAACAGACCT TTTACCCCAC CTACCAGTAC AGGTGGATCT
    AAATCGCCAG GCAGTGCAGG AAGATCTCAG ACTCCCCCAG GTGTTGCCAC ACCACCCATT
    CCCAAAATCA CTATTCAGAT TCCTAAGGGA ACAGTGATGG TGGGCAAGCC TTCCTCTCAC
    AGTCAGTATA CCAGCAGTGG TTCTGTGTCT TCCTCAGGCA GCAAAAGCCA CCATAGCCAT
    TCTTCCTCCT CTTCCTCATC TGCTTCCACC TCAGGGAAGA TGAAAAGCAG TAAATCAGAA
    GGTTCATCAA GTTCCAAGTT AAGTAGCAGT ATGTATTCTA GCCAGGGGTC TTCTGGATCT
    AGCCAGTCCA AAAATTCATC CCAGTCTGGG GGGAAGCCAG GCTCCTCTCC CATAACCAAG
    CATGGACTGA GCAGTGGCTC TAGCAGCACC AAGATGAAAC CTCAAGGAAA GCCATCATCA
    CTTATGAATC CTTCTTTAAG TAAACCAAAC ATATCCCCTT CTCATTCAAG GCCACCTGGA
    GGCTCTGACA AGCTTGCCTC TCCAATGAAG CCTGTTCCTG GAACTCCTCC ATCCTCTAAA
    GCCAAGTCCC CTATCAGTTC AGGTTCTGGT GGTTCTCATA TGTCTGGAAC TAGTTCAAGC
    TCTGGCATGA AGTCATCTTC AGGGTTAGGA TCCTCAGGCT CGTTGTCCCA GAAAACTCCC
    CCATCATCTA ATTCCTGTAC GGCATCTTCC TCCTCCTTTT CCTCAAGTGG CTCTTCCATG
    TCATCCTCTC AGAACCAGCA TGGGAGTTCT AAAGGAAAAT CTCCCAGCAG AAACAAGAAG
    CCGTCCTTGA CAGCTGTCAT AGATAAACTG AAGCATGGGG TTGTCACCAG TGGCCCTGGG
    GGTGAAGACC CACTGGACGG CCAGATGGGG GTGAGCACAA ATTCTTCCAG CCATCCTATG
    TCCTCCAAAC ATAACATGTC AGGAGGAGAG TTTCAGGGCA AGCGTGAGAA AAGTGATAAA
    GACAAATCAA AGGTTTCCAC CTCCGGGAGT TCAGTGGATT CTTCTAAGAA GACCTCAGAG
    TCAAAAAATG TGGGGAGCAC AGGTGTGGCA AAAATTATCA TCAGTAAGCA TGATGGAGGC
    TCCCCTAGCA TTAAAGCCAA AGTGACTTTG CAGAAACCTG GGGAAAGTAG TGGAGAAGGG
    CTTAGGCCTC AAATGGCTTC TTCTAAAAAC TATGGCTCTC CACTCATCAG TGGTTCCACT
    CCAAAGCATG AGCGTGGCTC TCCCAGCCAT AGTAAGTCAC CAGCATATAC CCCCCAGAAT
    CTGGACAGTG AAAGTGAGTC AGGCTCCTCC ATAGCAGAGA AATCTTATCA GAATAGTCCC
    AGCTCAGACG ATGGTATCCG ACCACTTCCA GAATACAGCA CAGAGAAACA TAAGAAGCAC
    AAAAAGGAAA AGAAGAAAGT AAAAGACAAA GATAGGGACC GAGACCGGGA CAAAGACCGA
    GACAAGAAAA AATCTCATAG CATCAAGCCA GAGAGTTGGT CCAAATCACC CATCTCTTCA
    GACCAGTCCT TGTCTATGAC AAGTAACACA ATCTTATCTG CAGACAGACC CTCAAGGCTC
    AGCCCAGACT TTATGATTGG GGAGGAAGAT GATGATCTTA TGGATGTGGC CCTGATTGGG
    AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17285
    Clone ID Related Accession (Same CDS sequence) NM_004774.4 , NM_004774.3
    Accession Version NM_004774.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4746bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-05
    Organism Homo sapiens(human)
    Product mediator of RNA polymerase II transcription subunit 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP357374.1, BC131783.1 and AC005288.1. This sequence is a reference standard in the RefSeqGene project. On Aug 7, 2007 this sequence version replaced NM_004774.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y13467.1, BC131783.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAAAGCTC AGGGGGAAAC CGAGGAGTCA GAAAAGCTGA GTAAGATGAG TTCTCTCCTG 
    GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCCTGGA GTGAAACCAT TAAGCTTGTG
    CGTCAAGTCA TGGAGAAGAG GGTTGTGATG AGTTCTGGAG GGCATCAACA TTTGGTCAGC
    TGTTTGGAGA CATTGCAGAA GGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGATCGT
    TTGGAGTCCA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA
    TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT
    TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCGGA GCTTGTACAG
    CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGCCT TGTTAATCTG
    TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA
    GAACAAGATC TTTCTAAAAT GGCAATTATG TACTGGAAAG CAACTAATGC TGGTCCCTTG
    GATAAGATTC TTCATGGAAG TGTTGGCTAT CTCACACCAA GGAGTGGGGG TCATTTAATG
    AACCTGAAGT ACTATGTCTC TCCTTCTGAC CTACTGGATG ACAAGACTGC ATCTCCCATC
    ATTTTGCATG AGAATAATGT TTCTCGATCT TTGGGCATGA ATGCATCAGT GACAATTGAA
    GGAACATCTG CTGTGTACAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT
    GACAATAAAT GGACCCCTTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT
    GCCTGTTTCT TCTTGAAATT TCCCCAGCCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA
    CTGCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGC ACCCCTGTAT
    GAACTGATCA CTCAGTTTGA GCTATCAAAG GACCCTGACC CCATACCTTT GAATCACAAC
    ATGAGATTTT ATGCTGCTCT TCCTGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT
    CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTTGTTA GCAAAATCAC CTTTCAGCAC
    CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACACC AAGTGGCCTA TAACACCCTC
    ATTGGAAGCT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT TCTCCAATTT
    GAAGTGTGTC CTCTCTCAGA GTCTCGTTTC AGCGTATCTT TTCAGCACCC TGTGAATGAC
    TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTCTAC
    AAAGGGCTGT CGGATGCACT GATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA
    TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAAGCC
    GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG
    CCCCCGGCTA GCAGCCCAGG GTATGGCATG ACCACAGGCA ACAACCCAAT GAGTGGTACC
    ACTACACCAA CCAACACCTT TCCGGGGGGT CCCATTACCA CCTTGTTTAA TATGAGCATG
    AGCATCAAAG ATCGGCATGA GTCGGTGGGC CATGGGGAGG ACTTCAGCAA GGTGTCTCAG
    AACCCAATTC TTACCAGTTT GTTGCAAATC ACAGGGAACG GGGGGTCTAC CATTGGCTCG
    AGTCCGACCC CTCCTCATCA CACGCCGCCA CCTGTCTCTT CGATGGCCGG CAACACCAAG
    AACCACCCGA TGCTCATGAA CCTTCTTAAA GATAATCCTG CCCAGGATTT CTCAACCCTT
    TATGGAAGCA GCCCTTTAGA AAGGCAGAAC TCCTCTTCCG GCTCACCCCG CATGGAAATA
    TGCTCGGGGA GCAACAAGAC CAAGAAAAAG AAGTCATCAA GATTACCACC TGAGAAACCA
    AAGCACCAGA CTGAAGATGA CTTTCAGAGG GAGCTATTTT CAATGGATGT TGACTCACAG
    AACCCTATCT TTGATGTCAA CATGACAGCT GACACGCTGG ATACGCCACA CATCACTCCA
    GCTCCAAGCC AGTGTAGCAC TCCCCCAACA ACTTACCCAC AACCAGTACC TCACCCCCAA
    CCCAGTATTC AAAGGATGGT CCGACTATCC AGTTCAGACA GCATTGGCCC AGATGTAACT
    GACATCCTTT CAGACATTGC AGAAGAAGCT TCTAAACTTC CCAGCACTAG TGATGATTGC
    CCAGCCATTG GCACCCCTCT TCGAGATTCT TCAAGCTCTG GGCATTCTCA GAGTACCCTG
    TTTGACTCTG ATGTCTTTCA AACTAACAAT AATGAAAATC CATACACTGA TCCAGCTGAT
    CTTATTGCAG ATGCTGCTGG AAGCCCCAGT AGTGACTCTC CTACCAATCA TTTTTTTCAT
    GATGGAGTAG ATTTCAATCC TGATTTATTG AACAGCCAGA GCCAAAGTGG TTTTGGAGAA
    GAATATTTTG ATGAAAGCAG CCAAAGTGGG GATAATGATG ATTTCAAAGG ATTTGCATCT
    CAGGCACTAA ATACTTTGGG GGTGCCAATG CTTGGAGGTG ATAATGGGGA GACCAAGTTT
    AAGGGCAATA ACCAAGCCGA CACAGTTGAT TTCAGTATTA TTTCAGTAGC CGGCAAAGCT
    TTAGCTCCTG CAGATCTTAT GGAGCATCAC AGTGGTAGTC AGGGTCCTTT ACTGACCACT
    GGGGACTTAG GGAAAGAAAA GACTCAAAAG AGGGTAAAGG AAGGCAATGG CACCAGTAAT
    AGTACTCTCT CGGGGCCCGG ATTAGACAGC AAACCAGGGA AGCGCAGTCG GACCCCTTCT
    AATGATGGGA AAAGCAAAGA TAAGCCTCCA AAGCGGAAGA AGGCAGACAC TGAGGGAAAG
    TCTCCATCTC ATAGTTCTTC TAACAGACCT TTTACCCCAC CTACCAGTAC AGGTGGATCT
    AAATCGCCAG GCAGTGCAGG AAGATCTCAG ACTCCCCCAG GTGTTGCCAC ACCACCCATT
    CCCAAAATCA CTATTCAGAT TCCTAAGGGA ACAGTGATGG TGGGCAAGCC TTCCTCTCAC
    AGTCAGTATA CCAGCAGTGG TTCTGTGTCT TCCTCAGGCA GCAAAAGCCA CCATAGCCAT
    TCTTCCTCCT CTTCCTCATC TGCTTCCACC TCAGGGAAGA TGAAAAGCAG TAAATCAGAA
    GGTTCATCAA GTTCCAAGTT AAGTAGCAGT ATGTATTCTA GCCAGGGGTC TTCTGGATCT
    AGCCAGTCCA AAAATTCATC CCAGTCTGGG GGGAAGCCAG GCTCCTCTCC CATAACCAAG
    CATGGACTGA GCAGTGGCTC TAGCAGCACC AAGATGAAAC CTCAAGGAAA GCCATCATCA
    CTTATGAATC CTTCTTTAAG TAAACCAAAC ATATCCCCTT CTCATTCAAG GCCACCTGGA
    GGCTCTGACA AGCTTGCCTC TCCAATGAAG CCTGTTCCTG GAACTCCTCC ATCCTCTAAA
    GCCAAGTCCC CTATCAGTTC AGGTTCTGGT GGTTCTCATA TGTCTGGAAC TAGTTCAAGC
    TCTGGCATGA AGTCATCTTC AGGGTTAGGA TCCTCAGGCT CGTTGTCCCA GAAAACTCCC
    CCATCATCTA ATTCCTGTAC GGCATCTTCC TCCTCCTTTT CCTCAAGTGG CTCTTCCATG
    TCATCCTCTC AGAACCAGCA TGGGAGTTCT AAAGGAAAAT CTCCCAGCAG AAACAAGAAG
    CCGTCCTTGA CAGCTGTCAT AGATAAACTG AAGCATGGGG TTGTCACCAG TGGCCCTGGG
    GGTGAAGACC CACTGGACGG CCAGATGGGG GTGAGCACAA ATTCTTCCAG CCATCCTATG
    TCCTCCAAAC ATAACATGTC AGGAGGAGAG TTTCAGGGCA AGCGTGAGAA AAGTGATAAA
    GACAAATCAA AGGTTTCCAC CTCCGGGAGT TCAGTGGATT CTTCTAAGAA GACCTCAGAG
    TCAAAAAATG TGGGGAGCAC AGGTGTGGCA AAAATTATCA TCAGTAAGCA TGATGGAGGC
    TCCCCTAGCA TTAAAGCCAA AGTGACTTTG CAGAAACCTG GGGAAAGTAG TGGAGAAGGG
    CTTAGGCCTC AAATGGCTTC TTCTAAAAAC TATGGCTCTC CACTCATCAG TGGTTCCACT
    CCAAAGCATG AGCGTGGCTC TCCCAGCCAT AGTAAGTCAC CAGCATATAC CCCCCAGAAT
    CTGGACAGTG AAAGTGAGTC AGGCTCCTCC ATAGCAGAGA AATCTTATCA GAATAGTCCC
    AGCTCAGACG ATGGTATCCG ACCACTTCCA GAATACAGCA CAGAGAAACA TAAGAAGCAC
    AAAAAGGAAA AGAAGAAAGT AAAAGACAAA GATAGGGACC GAGACCGGGA CAAAGACCGA
    GACAAGAAAA AATCTCATAG CATCAAGCCA GAGAGTTGGT CCAAATCACC CATCTCTTCA
    GACCAGTCCT TGTCTATGAC AAGTAACACA ATCTTATCTG CAGACAGACC CTCAAGGCTC
    AGCCCAGACT TTATGATTGG GGAGGAAGAT GATGATCTTA TGGATGTGGC CCTGATTGGG
    AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004765.2
    CDS213..4958
    Translation

    Target ORF information:

    RefSeq Version NM_004774.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mediator complex subunit 1 (MED1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004774.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAAAGCTC AGGGGGAAAC CGAGGAGTCA GAAAAGCTGA GTAAGATGAG TTCTCTCCTG 
    GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCCTGGA GTGAAACCAT TAAGCTTGTG
    CGTCAAGTCA TGGAGAAGAG GGTTGTGATG AGTTCTGGAG GGCATCAACA TTTGGTCAGC
    TGTTTGGAGA CATTGCAGAA GGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGATCGT
    TTGGAGTCCA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA
    TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT
    TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCGGA GCTTGTACAG
    CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGCCT TGTTAATCTG
    TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA
    GAACAAGATC TTTCTAAAAT GGCAATTATG TACTGGAAAG CAACTAATGC TGGTCCCTTG
    GATAAGATTC TTCATGGAAG TGTTGGCTAT CTCACACCAA GGAGTGGGGG TCATTTAATG
    AACCTGAAGT ACTATGTCTC TCCTTCTGAC CTACTGGATG ACAAGACTGC ATCTCCCATC
    ATTTTGCATG AGAATAATGT TTCTCGATCT TTGGGCATGA ATGCATCAGT GACAATTGAA
    GGAACATCTG CTGTGTACAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT
    GACAATAAAT GGACCCCTTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT
    GCCTGTTTCT TCTTGAAATT TCCCCAGCCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA
    CTGCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGC ACCCCTGTAT
    GAACTGATCA CTCAGTTTGA GCTATCAAAG GACCCTGACC CCATACCTTT GAATCACAAC
    ATGAGATTTT ATGCTGCTCT TCCTGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT
    CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTTGTTA GCAAAATCAC CTTTCAGCAC
    CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACACC AAGTGGCCTA TAACACCCTC
    ATTGGAAGCT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT TCTCCAATTT
    GAAGTGTGTC CTCTCTCAGA GTCTCGTTTC AGCGTATCTT TTCAGCACCC TGTGAATGAC
    TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTCTAC
    AAAGGGCTGT CGGATGCACT GATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA
    TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAAGCC
    GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG
    CCCCCGGCTA GCAGCCCAGG GTATGGCATG ACCACAGGCA ACAACCCAAT GAGTGGTACC
    ACTACACCAA CCAACACCTT TCCGGGGGGT CCCATTACCA CCTTGTTTAA TATGAGCATG
    AGCATCAAAG ATCGGCATGA GTCGGTGGGC CATGGGGAGG ACTTCAGCAA GGTGTCTCAG
    AACCCAATTC TTACCAGTTT GTTGCAAATC ACAGGGAACG GGGGGTCTAC CATTGGCTCG
    AGTCCGACCC CTCCTCATCA CACGCCGCCA CCTGTCTCTT CGATGGCCGG CAACACCAAG
    AACCACCCGA TGCTCATGAA CCTTCTTAAA GATAATCCTG CCCAGGATTT CTCAACCCTT
    TATGGAAGCA GCCCTTTAGA AAGGCAGAAC TCCTCTTCCG GCTCACCCCG CATGGAAATA
    TGCTCGGGGA GCAACAAGAC CAAGAAAAAG AAGTCATCAA GATTACCACC TGAGAAACCA
    AAGCACCAGA CTGAAGATGA CTTTCAGAGG GAGCTATTTT CAATGGATGT TGACTCACAG
    AACCCTATCT TTGATGTCAA CATGACAGCT GACACGCTGG ATACGCCACA CATCACTCCA
    GCTCCAAGCC AGTGTAGCAC TCCCCCAACA ACTTACCCAC AACCAGTACC TCACCCCCAA
    CCCAGTATTC AAAGGATGGT CCGACTATCC AGTTCAGACA GCATTGGCCC AGATGTAACT
    GACATCCTTT CAGACATTGC AGAAGAAGCT TCTAAACTTC CCAGCACTAG TGATGATTGC
    CCAGCCATTG GCACCCCTCT TCGAGATTCT TCAAGCTCTG GGCATTCTCA GAGTACCCTG
    TTTGACTCTG ATGTCTTTCA AACTAACAAT AATGAAAATC CATACACTGA TCCAGCTGAT
    CTTATTGCAG ATGCTGCTGG AAGCCCCAGT AGTGACTCTC CTACCAATCA TTTTTTTCAT
    GATGGAGTAG ATTTCAATCC TGATTTATTG AACAGCCAGA GCCAAAGTGG TTTTGGAGAA
    GAATATTTTG ATGAAAGCAG CCAAAGTGGG GATAATGATG ATTTCAAAGG ATTTGCATCT
    CAGGCACTAA ATACTTTGGG GGTGCCAATG CTTGGAGGTG ATAATGGGGA GACCAAGTTT
    AAGGGCAATA ACCAAGCCGA CACAGTTGAT TTCAGTATTA TTTCAGTAGC CGGCAAAGCT
    TTAGCTCCTG CAGATCTTAT GGAGCATCAC AGTGGTAGTC AGGGTCCTTT ACTGACCACT
    GGGGACTTAG GGAAAGAAAA GACTCAAAAG AGGGTAAAGG AAGGCAATGG CACCAGTAAT
    AGTACTCTCT CGGGGCCCGG ATTAGACAGC AAACCAGGGA AGCGCAGTCG GACCCCTTCT
    AATGATGGGA AAAGCAAAGA TAAGCCTCCA AAGCGGAAGA AGGCAGACAC TGAGGGAAAG
    TCTCCATCTC ATAGTTCTTC TAACAGACCT TTTACCCCAC CTACCAGTAC AGGTGGATCT
    AAATCGCCAG GCAGTGCAGG AAGATCTCAG ACTCCCCCAG GTGTTGCCAC ACCACCCATT
    CCCAAAATCA CTATTCAGAT TCCTAAGGGA ACAGTGATGG TGGGCAAGCC TTCCTCTCAC
    AGTCAGTATA CCAGCAGTGG TTCTGTGTCT TCCTCAGGCA GCAAAAGCCA CCATAGCCAT
    TCTTCCTCCT CTTCCTCATC TGCTTCCACC TCAGGGAAGA TGAAAAGCAG TAAATCAGAA
    GGTTCATCAA GTTCCAAGTT AAGTAGCAGT ATGTATTCTA GCCAGGGGTC TTCTGGATCT
    AGCCAGTCCA AAAATTCATC CCAGTCTGGG GGGAAGCCAG GCTCCTCTCC CATAACCAAG
    CATGGACTGA GCAGTGGCTC TAGCAGCACC AAGATGAAAC CTCAAGGAAA GCCATCATCA
    CTTATGAATC CTTCTTTAAG TAAACCAAAC ATATCCCCTT CTCATTCAAG GCCACCTGGA
    GGCTCTGACA AGCTTGCCTC TCCAATGAAG CCTGTTCCTG GAACTCCTCC ATCCTCTAAA
    GCCAAGTCCC CTATCAGTTC AGGTTCTGGT GGTTCTCATA TGTCTGGAAC TAGTTCAAGC
    TCTGGCATGA AGTCATCTTC AGGGTTAGGA TCCTCAGGCT CGTTGTCCCA GAAAACTCCC
    CCATCATCTA ATTCCTGTAC GGCATCTTCC TCCTCCTTTT CCTCAAGTGG CTCTTCCATG
    TCATCCTCTC AGAACCAGCA TGGGAGTTCT AAAGGAAAAT CTCCCAGCAG AAACAAGAAG
    CCGTCCTTGA CAGCTGTCAT AGATAAACTG AAGCATGGGG TTGTCACCAG TGGCCCTGGG
    GGTGAAGACC CACTGGACGG CCAGATGGGG GTGAGCACAA ATTCTTCCAG CCATCCTATG
    TCCTCCAAAC ATAACATGTC AGGAGGAGAG TTTCAGGGCA AGCGTGAGAA AAGTGATAAA
    GACAAATCAA AGGTTTCCAC CTCCGGGAGT TCAGTGGATT CTTCTAAGAA GACCTCAGAG
    TCAAAAAATG TGGGGAGCAC AGGTGTGGCA AAAATTATCA TCAGTAAGCA TGATGGAGGC
    TCCCCTAGCA TTAAAGCCAA AGTGACTTTG CAGAAACCTG GGGAAAGTAG TGGAGAAGGG
    CTTAGGCCTC AAATGGCTTC TTCTAAAAAC TATGGCTCTC CACTCATCAG TGGTTCCACT
    CCAAAGCATG AGCGTGGCTC TCCCAGCCAT AGTAAGTCAC CAGCATATAC CCCCCAGAAT
    CTGGACAGTG AAAGTGAGTC AGGCTCCTCC ATAGCAGAGA AATCTTATCA GAATAGTCCC
    AGCTCAGACG ATGGTATCCG ACCACTTCCA GAATACAGCA CAGAGAAACA TAAGAAGCAC
    AAAAAGGAAA AGAAGAAAGT AAAAGACAAA GATAGGGACC GAGACCGGGA CAAAGACCGA
    GACAAGAAAA AATCTCATAG CATCAAGCCA GAGAGTTGGT CCAAATCACC CATCTCTTCA
    GACCAGTCCT TGTCTATGAC AAGTAACACA ATCTTATCTG CAGACAGACC CTCAAGGCTC
    AGCCCAGACT TTATGATTGG GGAGGAAGAT GATGATCTTA TGGATGTGGC CCTGATTGGG
    AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu50485
    Clone ID Related Accession (Same CDS sequence) XM_006721957.1 , XM_006721957.2
    Accession Version XM_006721957.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1851bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product mediator of RNA polymerase II transcription subunit 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAAAGCTC AGGGGGAAAC CGAGGAGTCA GAAAAGCTGA GTAAGATGAG TTCTCTCCTG 
    GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCCTGGA GTGAAACCAT TAAGCTTGTG
    CGTCAAGTCA TGGAGAAGAG GGTTGTGATG AGTTCTGGAG GGCATCAACA TTTGGTCAGC
    TGTTTGGAGA CATTGCAGAA GGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGATCGT
    TTGGAGTCCA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA
    TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT
    TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCGGA GCTTGTACAG
    CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGCCT TGTTAATCTG
    TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA
    GAACAAGATC TTTCTAAAAT GGCAATTATG TACTGGAAAG CAACTAATGC TGGTCCCTTG
    GATAAGATTC TTCATGGAAG TGTTGGCTAT CTCACACCAA GGAGTGGGGG TCATTTAATG
    AACCTGAAGT ACTATGTCTC TCCTTCTGAC CTACTGGATG ACAAGACTGC ATCTCCCATC
    ATTTTGCATG AGAATAATGT TTCTCGATCT TTGGGCATGA ATGCATCAGT GACAATTGAA
    GGAACATCTG CTGTGTACAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT
    GACAATAAAT GGACCCCTTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT
    GCCTGTTTCT TCTTGAAATT TCCCCAGCCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA
    CTGCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGC ACCCCTGTAT
    GAACTGATCA CTCAGTTTGA GCTATCAAAG GACCCTGACC CCATACCTTT GAATCACAAC
    ATGAGATTTT ATGCTGCTCT TCCTGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT
    CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTTGTTA GCAAAATCAC CTTTCAGCAC
    CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACACC AAGTGGCCTA TAACACCCTC
    ATTGGAAGCT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT TCTCCAATTT
    GAAGTGTGTC CTCTCTCAGA GTCTCGTTTC AGCGTATCTT TTCAGCACCC TGTGAATGAC
    TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTCTAC
    AAAGGGCTGT CGGATGCACT GATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA
    TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAAGCC
    GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG
    CCCCCGGCTA GCAGCCCAGG GGAGCCGGGG CTAAACTGCT TCACCCTGCC TGAGAACCAG
    GGAGCACTGC ATTTCTCCAC AGGGTGGAGG AGAAGAGGCA GAATAAACCA AGCCTGGGAC
    ACCTCCCTCC TGTCTAGGTG TACTCATTCT TCTGTTTCAA AAGAAGGCAA GGACATGAAG
    TCAACTTCTA CCTATCTTCT GCTGCTGGTG TCTTATGTAT TCTCAGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006722020.1
    CDS210..2060
    Translation

    Target ORF information:

    RefSeq Version XM_006721957.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mediator complex subunit 1 (MED1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006721957.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAAAGCTC AGGGGGAAAC CGAGGAGTCA GAAAAGCTGA GTAAGATGAG TTCTCTCCTG 
    GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCCTGGA GTGAAACCAT TAAGCTTGTG
    CGTCAAGTCA TGGAGAAGAG GGTTGTGATG AGTTCTGGAG GGCATCAACA TTTGGTCAGC
    TGTTTGGAGA CATTGCAGAA GGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGATCGT
    TTGGAGTCCA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA
    TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT
    TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCGGA GCTTGTACAG
    CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGCCT TGTTAATCTG
    TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA
    GAACAAGATC TTTCTAAAAT GGCAATTATG TACTGGAAAG CAACTAATGC TGGTCCCTTG
    GATAAGATTC TTCATGGAAG TGTTGGCTAT CTCACACCAA GGAGTGGGGG TCATTTAATG
    AACCTGAAGT ACTATGTCTC TCCTTCTGAC CTACTGGATG ACAAGACTGC ATCTCCCATC
    ATTTTGCATG AGAATAATGT TTCTCGATCT TTGGGCATGA ATGCATCAGT GACAATTGAA
    GGAACATCTG CTGTGTACAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT
    GACAATAAAT GGACCCCTTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT
    GCCTGTTTCT TCTTGAAATT TCCCCAGCCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA
    CTGCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGC ACCCCTGTAT
    GAACTGATCA CTCAGTTTGA GCTATCAAAG GACCCTGACC CCATACCTTT GAATCACAAC
    ATGAGATTTT ATGCTGCTCT TCCTGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT
    CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTTGTTA GCAAAATCAC CTTTCAGCAC
    CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACACC AAGTGGCCTA TAACACCCTC
    ATTGGAAGCT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT TCTCCAATTT
    GAAGTGTGTC CTCTCTCAGA GTCTCGTTTC AGCGTATCTT TTCAGCACCC TGTGAATGAC
    TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTCTAC
    AAAGGGCTGT CGGATGCACT GATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA
    TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAAGCC
    GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG
    CCCCCGGCTA GCAGCCCAGG GGAGCCGGGG CTAAACTGCT TCACCCTGCC TGAGAACCAG
    GGAGCACTGC ATTTCTCCAC AGGGTGGAGG AGAAGAGGCA GAATAAACCA AGCCTGGGAC
    ACCTCCCTCC TGTCTAGGTG TACTCATTCT TCTGTTTCAA AAGAAGGCAA GGACATGAAG
    TCAACTTCTA CCTATCTTCT GCTGCTGGTG TCTTATGTAT TCTCAGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu50485
    Clone ID Related Accession (Same CDS sequence) XM_006721957.1 , XM_006721957.2
    Accession Version XM_006721957.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1851bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mediator of RNA polymerase II transcription subunit 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006721957.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAAAGCTC AGGGGGAAAC CGAGGAGTCA GAAAAGCTGA GTAAGATGAG TTCTCTCCTG 
    GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCCTGGA GTGAAACCAT TAAGCTTGTG
    CGTCAAGTCA TGGAGAAGAG GGTTGTGATG AGTTCTGGAG GGCATCAACA TTTGGTCAGC
    TGTTTGGAGA CATTGCAGAA GGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGATCGT
    TTGGAGTCCA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA
    TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT
    TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCGGA GCTTGTACAG
    CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGCCT TGTTAATCTG
    TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA
    GAACAAGATC TTTCTAAAAT GGCAATTATG TACTGGAAAG CAACTAATGC TGGTCCCTTG
    GATAAGATTC TTCATGGAAG TGTTGGCTAT CTCACACCAA GGAGTGGGGG TCATTTAATG
    AACCTGAAGT ACTATGTCTC TCCTTCTGAC CTACTGGATG ACAAGACTGC ATCTCCCATC
    ATTTTGCATG AGAATAATGT TTCTCGATCT TTGGGCATGA ATGCATCAGT GACAATTGAA
    GGAACATCTG CTGTGTACAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT
    GACAATAAAT GGACCCCTTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT
    GCCTGTTTCT TCTTGAAATT TCCCCAGCCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA
    CTGCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGC ACCCCTGTAT
    GAACTGATCA CTCAGTTTGA GCTATCAAAG GACCCTGACC CCATACCTTT GAATCACAAC
    ATGAGATTTT ATGCTGCTCT TCCTGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT
    CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTTGTTA GCAAAATCAC CTTTCAGCAC
    CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACACC AAGTGGCCTA TAACACCCTC
    ATTGGAAGCT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT TCTCCAATTT
    GAAGTGTGTC CTCTCTCAGA GTCTCGTTTC AGCGTATCTT TTCAGCACCC TGTGAATGAC
    TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTCTAC
    AAAGGGCTGT CGGATGCACT GATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA
    TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAAGCC
    GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG
    CCCCCGGCTA GCAGCCCAGG GGAGCCGGGG CTAAACTGCT TCACCCTGCC TGAGAACCAG
    GGAGCACTGC ATTTCTCCAC AGGGTGGAGG AGAAGAGGCA GAATAAACCA AGCCTGGGAC
    ACCTCCCTCC TGTCTAGGTG TACTCATTCT TCTGTTTCAA AAGAAGGCAA GGACATGAAG
    TCAACTTCTA CCTATCTTCT GCTGCTGGTG TCTTATGTAT TCTCAGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006722020.1
    CDS213..2063
    Translation

    Target ORF information:

    RefSeq Version XM_006721957.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mediator complex subunit 1 (MED1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006721957.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAAAGCTC AGGGGGAAAC CGAGGAGTCA GAAAAGCTGA GTAAGATGAG TTCTCTCCTG 
    GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCCTGGA GTGAAACCAT TAAGCTTGTG
    CGTCAAGTCA TGGAGAAGAG GGTTGTGATG AGTTCTGGAG GGCATCAACA TTTGGTCAGC
    TGTTTGGAGA CATTGCAGAA GGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGATCGT
    TTGGAGTCCA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA
    TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT
    TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCGGA GCTTGTACAG
    CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGCCT TGTTAATCTG
    TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA
    GAACAAGATC TTTCTAAAAT GGCAATTATG TACTGGAAAG CAACTAATGC TGGTCCCTTG
    GATAAGATTC TTCATGGAAG TGTTGGCTAT CTCACACCAA GGAGTGGGGG TCATTTAATG
    AACCTGAAGT ACTATGTCTC TCCTTCTGAC CTACTGGATG ACAAGACTGC ATCTCCCATC
    ATTTTGCATG AGAATAATGT TTCTCGATCT TTGGGCATGA ATGCATCAGT GACAATTGAA
    GGAACATCTG CTGTGTACAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT
    GACAATAAAT GGACCCCTTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT
    GCCTGTTTCT TCTTGAAATT TCCCCAGCCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA
    CTGCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGC ACCCCTGTAT
    GAACTGATCA CTCAGTTTGA GCTATCAAAG GACCCTGACC CCATACCTTT GAATCACAAC
    ATGAGATTTT ATGCTGCTCT TCCTGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT
    CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTTGTTA GCAAAATCAC CTTTCAGCAC
    CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACACC AAGTGGCCTA TAACACCCTC
    ATTGGAAGCT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT TCTCCAATTT
    GAAGTGTGTC CTCTCTCAGA GTCTCGTTTC AGCGTATCTT TTCAGCACCC TGTGAATGAC
    TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTCTAC
    AAAGGGCTGT CGGATGCACT GATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA
    TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAAGCC
    GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG
    CCCCCGGCTA GCAGCCCAGG GGAGCCGGGG CTAAACTGCT TCACCCTGCC TGAGAACCAG
    GGAGCACTGC ATTTCTCCAC AGGGTGGAGG AGAAGAGGCA GAATAAACCA AGCCTGGGAC
    ACCTCCCTCC TGTCTAGGTG TACTCATTCT TCTGTTTCAA AAGAAGGCAA GGACATGAAG
    TCAACTTCTA CCTATCTTCT GCTGCTGGTG TCTTATGTAT TCTCAGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu86938
    Clone ID Related Accession (Same CDS sequence) XM_017024779.1 , XM_017024779.2
    Accession Version XM_017024779.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product mediator of RNA polymerase II transcription subunit 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAACCTGA AGTACTATGT CTCTCCTTCT GACCTACTGG ATGACAAGAC TGCATCTCCC 
    ATCATTTTGC ATGAGAATAA TGTTTCTCGA TCTTTGGGCA TGAATGCATC AGTGACAATT
    GAAGGAACAT CTGCTGTGTA CAAACTCCCA ATTGCACCAT TAATTATGGG GTCACATCCA
    GTTGACAATA AATGGACCCC TTCCTTCTCC TCAATCACCA GTGCCAACAG TGTTGATCTT
    CCTGCCTGTT TCTTCTTGAA ATTTCCCCAG CCAATCCCAG TATCTAGAGC ATTTGTTCAG
    AAACTGCAGA ACTGCACAGG AATTCCATTG TTTGAAACTC AACCAACTTA TGCACCCCTG
    TATGAACTGA TCACTCAGTT TGAGCTATCA AAGGACCCTG ACCCCATACC TTTGAATCAC
    AACATGAGAT TTTATGCTGC TCTTCCTGGT CAGCAGCACT GCTATTTCCT CAACAAGGAT
    GCTCCTCTTC CAGATGGCCG AAGTCTACAG GGAACCCTTG TTAGCAAAAT CACCTTTCAG
    CACCCTGGCC GAGTTCCTCT TATCCTAAAT CTGATCAGAC ACCAAGTGGC CTATAACACC
    CTCATTGGAA GCTGTGTCAA AAGAACTATT CTGAAAGAAG ATTCTCCTGG GCTTCTCCAA
    TTTGAAGTGT GTCCTCTCTC AGAGTCTCGT TTCAGCGTAT CTTTTCAGCA CCCTGTGAAT
    GACTCCCTGG TGTGTGTGGT AATGGATGTG CAGGACTCAA CACATGTGAG CTGTAAACTC
    TACAAAGGGC TGTCGGATGC ACTGATCTGC ACAGATGACT TCATTGCCAA AGTTGTTCAA
    AGATGTATGT CCATCCCTGT GACGATGAGG GCTATTCGGA GGAAAGCTGA AACCATTCAA
    GCCGACACCC CAGCACTGTC CCTCATTGCA GAGACAGTTG AAGACATGGT GAAAAAGAAC
    CTGCCCCCGG CTAGCAGCCC AGGGTATGGC ATGACCACAG GCAACAACCC AATGAGTGGT
    ACCACTACAC CAACCAACAC CTTTCCGGGG GGTCCCATTA CCACCTTGTT TAATATGAGC
    ATGAGCATCA AAGATCGGCA TGAGTCGGTG GGCCATGGGG AGGACTTCAG CAAGGTGTCT
    CAGAACCCAA TTCTTACCAG TTTGTTGCAA ATCACAGGGA ACGGGGGGTC TACCATTGGC
    TCGAGTCCGA CCCCTCCTCA TCACACGCCG CCACCTGTCT CTTCGATGGC CGGCAACACC
    AAGAACCACC CGATGCTCAT GAACCTTCTT AAAGATAATC CTGCCCAGGA TTTCTCAACC
    CTTTATGGAA GCAGCCCTTT AGAAAGGCAG AACTCCTCTT CCGGCTCACC CCGCATGGAA
    ATATGCTCGG GGAGCAACAA GACCAAGAAA AAGAAGTCAT CAAGATTACC ACCTGAGAAA
    CCAAAGCACC AGACTGAAGA TGACTTTCAG AGGGAGCTAT TTTCAATGGA TGTTGACTCA
    CAGAACCCTA TCTTTGATGT CAACATGACA GCTGACACGC TGGATACGCC ACACATCACT
    CCAGCTCCAA GCCAGTGTAG CACTCCCCCA ACAACTTACC CACAACCAGT ACCTCACCCC
    CAACCCAGTA TTCAAAGGAT GGTCCGACTA TCCAGTTCAG ACAGCATTGG CCCAGATGTA
    ACTGACATCC TTTCAGACAT TGCAGAAGAA GCTTCTAAAC TTCCCAGCAC TAGTGATGAT
    TGCCCAGCCA TTGGCACCCC TCTTCGAGAT TCTTCAAGCT CTGGGCATTC TCAGAGTACC
    CTGTTTGACT CTGATGTCTT TCAAACTAAC AATAATGAAA ATCCATACAC TGATCCAGCT
    GATCTTATTG CAGATGCTGC TGGAAGCCCC AGTAGTGACT CTCCTACCAA TCATTTTTTT
    CATGATGGAG TAGATTTCAA TCCTGATTTA TTGAACAGCC AGAGCCAAAG TGGTTTTGGA
    GAAGAATATT TTGATGAAAG CAGCCAAAGT GGGGATAATG ATGATTTCAA AGGATTTGCA
    TCTCAGGCAC TAAATACTTT GGGGGTGCCA ATGCTTGGAG GTGATAATGG GGAGACCAAG
    TTTAAGGGCA ATAACCAAGC CGACACAGTT GATTTCAGTA TTATTTCAGT AGCCGGCAAA
    GCTTTAGCTC CTGCAGATCT TATGGAGCAT CACAGTGGTA GTCAGGGTCC TTTACTGACC
    ACTGGGGACT TAGGGAAAGA AAAGACTCAA AAGAGGGTAA AGGAAGGCAA TGGCACCAGT
    AATAGTACTC TCTCGGGGCC CGGATTAGAC AGCAAACCAG GGAAGCGCAG TCGGACCCCT
    TCTAATGATG GGAAAAGCAA AGATAAGCCT CCAAAGCGGA AGAAGGCAGA CACTGAGGGA
    AAGTCTCCAT CTCATAGTTC TTCTAACAGA CCTTTTACCC CACCTACCAG TACAGGTGGA
    TCTAAATCGC CAGGCAGTGC AGGAAGATCT CAGACTCCCC CAGGTGTTGC CACACCACCC
    ATTCCCAAAA TCACTATTCA GATTCCTAAG GGAACAGTGA TGGTGGGCAA GCCTTCCTCT
    CACAGTCAGT ATACCAGCAG TGGTTCTGTG TCTTCCTCAG GCAGCAAAAG CCACCATAGC
    CATTCTTCCT CCTCTTCCTC ATCTGCTTCC ACCTCAGGGA AGATGAAAAG CAGTAAATCA
    GAAGGTTCAT CAAGTTCCAA GTTAAGTAGC AGTATGTATT CTAGCCAGGG GTCTTCTGGA
    TCTAGCCAGT CCAAAAATTC ATCCCAGTCT GGGGGGAAGC CAGGCTCCTC TCCCATAACC
    AAGCATGGAC TGAGCAGTGG CTCTAGCAGC ACCAAGATGA AACCTCAAGG AAAGCCATCA
    TCACTTATGA ATCCTTCTTT AAGTAAACCA AACATATCCC CTTCTCATTC AAGGCCACCT
    GGAGGCTCTG ACAAGCTTGC CTCTCCAATG AAGCCTGTTC CTGGAACTCC TCCATCCTCT
    AAAGCCAAGT CCCCTATCAG TTCAGGTTCT GGTGGTTCTC ATATGTCTGG AACTAGTTCA
    AGCTCTGGCA TGAAGTCATC TTCAGGGTTA GGATCCTCAG GCTCGTTGTC CCAGAAAACT
    CCCCCATCAT CTAATTCCTG TACGGCATCT TCCTCCTCCT TTTCCTCAAG TGGCTCTTCC
    ATGTCATCCT CTCAGAACCA GCATGGGAGT TCTAAAGGAA AATCTCCCAG CAGAAACAAG
    AAGCCGTCCT TGACAGCTGT CATAGATAAA CTGAAGCATG GGGTTGTCAC CAGTGGCCCT
    GGGGGTGAAG ACCCACTGGA CGGCCAGATG GGGGTGAGCA CAAATTCTTC CAGCCATCCT
    ATGTCCTCCA AACATAACAT GTCAGGAGGA GAGTTTCAGG GCAAGCGTGA GAAAAGTGAT
    AAAGACAAAT CAAAGGTTTC CACCTCCGGG AGTTCAGTGG ATTCTTCTAA GAAGACCTCA
    GAGTCAAAAA ATGTGGGGAG CACAGGTGTG GCAAAAATTA TCATCAGTAA GCATGATGGA
    GGCTCCCCTA GCATTAAAGC CAAAGTGACT TTGCAGAAAC CTGGGGAAAG TAGTGGAGAA
    GGGCTTAGGC CTCAAATGGC TTCTTCTAAA AACTATGGCT CTCCACTCAT CAGTGGTTCC
    ACTCCAAAGC ATGAGCGTGG CTCTCCCAGC CATAGTAAGT CACCAGCATA TACCCCCCAG
    AATCTGGACA GTGAAAGTGA GTCAGGCTCC TCCATAGCAG AGAAATCTTA TCAGAATAGT
    CCCAGCTCAG ACGATGGTAT CCGACCACTT CCAGAATACA GCACAGAGAA ACATAAGAAG
    CACAAAAAGG AAAAGAAGAA AGTAAAAGAC AAAGATAGGG ACCGAGACCG GGACAAAGAC
    CGAGACAAGA AAAAATCTCA TAGCATCAAG CCAGAGAGTT GGTCCAAATC ACCCATCTCT
    TCAGACCAGT CCTTGTCTAT GACAAGTAAC ACAATCTTAT CTGCAGACAG ACCCTCAAGG
    CTCAGCCCAG ACTTTATGAT TGGGGAGGAA GATGATGATC TTATGGATGT GGCCCTGATT
    GGGAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016880268.1
    CDS803..4891
    Translation

    Target ORF information:

    RefSeq Version XM_017024779.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mediator complex subunit 1 (MED1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017024779.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAACCTGA AGTACTATGT CTCTCCTTCT GACCTACTGG ATGACAAGAC TGCATCTCCC 
    ATCATTTTGC ATGAGAATAA TGTTTCTCGA TCTTTGGGCA TGAATGCATC AGTGACAATT
    GAAGGAACAT CTGCTGTGTA CAAACTCCCA ATTGCACCAT TAATTATGGG GTCACATCCA
    GTTGACAATA AATGGACCCC TTCCTTCTCC TCAATCACCA GTGCCAACAG TGTTGATCTT
    CCTGCCTGTT TCTTCTTGAA ATTTCCCCAG CCAATCCCAG TATCTAGAGC ATTTGTTCAG
    AAACTGCAGA ACTGCACAGG AATTCCATTG TTTGAAACTC AACCAACTTA TGCACCCCTG
    TATGAACTGA TCACTCAGTT TGAGCTATCA AAGGACCCTG ACCCCATACC TTTGAATCAC
    AACATGAGAT TTTATGCTGC TCTTCCTGGT CAGCAGCACT GCTATTTCCT CAACAAGGAT
    GCTCCTCTTC CAGATGGCCG AAGTCTACAG GGAACCCTTG TTAGCAAAAT CACCTTTCAG
    CACCCTGGCC GAGTTCCTCT TATCCTAAAT CTGATCAGAC ACCAAGTGGC CTATAACACC
    CTCATTGGAA GCTGTGTCAA AAGAACTATT CTGAAAGAAG ATTCTCCTGG GCTTCTCCAA
    TTTGAAGTGT GTCCTCTCTC AGAGTCTCGT TTCAGCGTAT CTTTTCAGCA CCCTGTGAAT
    GACTCCCTGG TGTGTGTGGT AATGGATGTG CAGGACTCAA CACATGTGAG CTGTAAACTC
    TACAAAGGGC TGTCGGATGC ACTGATCTGC ACAGATGACT TCATTGCCAA AGTTGTTCAA
    AGATGTATGT CCATCCCTGT GACGATGAGG GCTATTCGGA GGAAAGCTGA AACCATTCAA
    GCCGACACCC CAGCACTGTC CCTCATTGCA GAGACAGTTG AAGACATGGT GAAAAAGAAC
    CTGCCCCCGG CTAGCAGCCC AGGGTATGGC ATGACCACAG GCAACAACCC AATGAGTGGT
    ACCACTACAC CAACCAACAC CTTTCCGGGG GGTCCCATTA CCACCTTGTT TAATATGAGC
    ATGAGCATCA AAGATCGGCA TGAGTCGGTG GGCCATGGGG AGGACTTCAG CAAGGTGTCT
    CAGAACCCAA TTCTTACCAG TTTGTTGCAA ATCACAGGGA ACGGGGGGTC TACCATTGGC
    TCGAGTCCGA CCCCTCCTCA TCACACGCCG CCACCTGTCT CTTCGATGGC CGGCAACACC
    AAGAACCACC CGATGCTCAT GAACCTTCTT AAAGATAATC CTGCCCAGGA TTTCTCAACC
    CTTTATGGAA GCAGCCCTTT AGAAAGGCAG AACTCCTCTT CCGGCTCACC CCGCATGGAA
    ATATGCTCGG GGAGCAACAA GACCAAGAAA AAGAAGTCAT CAAGATTACC ACCTGAGAAA
    CCAAAGCACC AGACTGAAGA TGACTTTCAG AGGGAGCTAT TTTCAATGGA TGTTGACTCA
    CAGAACCCTA TCTTTGATGT CAACATGACA GCTGACACGC TGGATACGCC ACACATCACT
    CCAGCTCCAA GCCAGTGTAG CACTCCCCCA ACAACTTACC CACAACCAGT ACCTCACCCC
    CAACCCAGTA TTCAAAGGAT GGTCCGACTA TCCAGTTCAG ACAGCATTGG CCCAGATGTA
    ACTGACATCC TTTCAGACAT TGCAGAAGAA GCTTCTAAAC TTCCCAGCAC TAGTGATGAT
    TGCCCAGCCA TTGGCACCCC TCTTCGAGAT TCTTCAAGCT CTGGGCATTC TCAGAGTACC
    CTGTTTGACT CTGATGTCTT TCAAACTAAC AATAATGAAA ATCCATACAC TGATCCAGCT
    GATCTTATTG CAGATGCTGC TGGAAGCCCC AGTAGTGACT CTCCTACCAA TCATTTTTTT
    CATGATGGAG TAGATTTCAA TCCTGATTTA TTGAACAGCC AGAGCCAAAG TGGTTTTGGA
    GAAGAATATT TTGATGAAAG CAGCCAAAGT GGGGATAATG ATGATTTCAA AGGATTTGCA
    TCTCAGGCAC TAAATACTTT GGGGGTGCCA ATGCTTGGAG GTGATAATGG GGAGACCAAG
    TTTAAGGGCA ATAACCAAGC CGACACAGTT GATTTCAGTA TTATTTCAGT AGCCGGCAAA
    GCTTTAGCTC CTGCAGATCT TATGGAGCAT CACAGTGGTA GTCAGGGTCC TTTACTGACC
    ACTGGGGACT TAGGGAAAGA AAAGACTCAA AAGAGGGTAA AGGAAGGCAA TGGCACCAGT
    AATAGTACTC TCTCGGGGCC CGGATTAGAC AGCAAACCAG GGAAGCGCAG TCGGACCCCT
    TCTAATGATG GGAAAAGCAA AGATAAGCCT CCAAAGCGGA AGAAGGCAGA CACTGAGGGA
    AAGTCTCCAT CTCATAGTTC TTCTAACAGA CCTTTTACCC CACCTACCAG TACAGGTGGA
    TCTAAATCGC CAGGCAGTGC AGGAAGATCT CAGACTCCCC CAGGTGTTGC CACACCACCC
    ATTCCCAAAA TCACTATTCA GATTCCTAAG GGAACAGTGA TGGTGGGCAA GCCTTCCTCT
    CACAGTCAGT ATACCAGCAG TGGTTCTGTG TCTTCCTCAG GCAGCAAAAG CCACCATAGC
    CATTCTTCCT CCTCTTCCTC ATCTGCTTCC ACCTCAGGGA AGATGAAAAG CAGTAAATCA
    GAAGGTTCAT CAAGTTCCAA GTTAAGTAGC AGTATGTATT CTAGCCAGGG GTCTTCTGGA
    TCTAGCCAGT CCAAAAATTC ATCCCAGTCT GGGGGGAAGC CAGGCTCCTC TCCCATAACC
    AAGCATGGAC TGAGCAGTGG CTCTAGCAGC ACCAAGATGA AACCTCAAGG AAAGCCATCA
    TCACTTATGA ATCCTTCTTT AAGTAAACCA AACATATCCC CTTCTCATTC AAGGCCACCT
    GGAGGCTCTG ACAAGCTTGC CTCTCCAATG AAGCCTGTTC CTGGAACTCC TCCATCCTCT
    AAAGCCAAGT CCCCTATCAG TTCAGGTTCT GGTGGTTCTC ATATGTCTGG AACTAGTTCA
    AGCTCTGGCA TGAAGTCATC TTCAGGGTTA GGATCCTCAG GCTCGTTGTC CCAGAAAACT
    CCCCCATCAT CTAATTCCTG TACGGCATCT TCCTCCTCCT TTTCCTCAAG TGGCTCTTCC
    ATGTCATCCT CTCAGAACCA GCATGGGAGT TCTAAAGGAA AATCTCCCAG CAGAAACAAG
    AAGCCGTCCT TGACAGCTGT CATAGATAAA CTGAAGCATG GGGTTGTCAC CAGTGGCCCT
    GGGGGTGAAG ACCCACTGGA CGGCCAGATG GGGGTGAGCA CAAATTCTTC CAGCCATCCT
    ATGTCCTCCA AACATAACAT GTCAGGAGGA GAGTTTCAGG GCAAGCGTGA GAAAAGTGAT
    AAAGACAAAT CAAAGGTTTC CACCTCCGGG AGTTCAGTGG ATTCTTCTAA GAAGACCTCA
    GAGTCAAAAA ATGTGGGGAG CACAGGTGTG GCAAAAATTA TCATCAGTAA GCATGATGGA
    GGCTCCCCTA GCATTAAAGC CAAAGTGACT TTGCAGAAAC CTGGGGAAAG TAGTGGAGAA
    GGGCTTAGGC CTCAAATGGC TTCTTCTAAA AACTATGGCT CTCCACTCAT CAGTGGTTCC
    ACTCCAAAGC ATGAGCGTGG CTCTCCCAGC CATAGTAAGT CACCAGCATA TACCCCCCAG
    AATCTGGACA GTGAAAGTGA GTCAGGCTCC TCCATAGCAG AGAAATCTTA TCAGAATAGT
    CCCAGCTCAG ACGATGGTAT CCGACCACTT CCAGAATACA GCACAGAGAA ACATAAGAAG
    CACAAAAAGG AAAAGAAGAA AGTAAAAGAC AAAGATAGGG ACCGAGACCG GGACAAAGAC
    CGAGACAAGA AAAAATCTCA TAGCATCAAG CCAGAGAGTT GGTCCAAATC ACCCATCTCT
    TCAGACCAGT CCTTGTCTAT GACAAGTAAC ACAATCTTAT CTGCAGACAG ACCCTCAAGG
    CTCAGCCCAG ACTTTATGAT TGGGGAGGAA GATGATGATC TTATGGATGT GGCCCTGATT
    GGGAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu86938
    Clone ID Related Accession (Same CDS sequence) XM_017024779.1 , XM_017024779.2
    Accession Version XM_017024779.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mediator of RNA polymerase II transcription subunit 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017024779.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAACCTGA AGTACTATGT CTCTCCTTCT GACCTACTGG ATGACAAGAC TGCATCTCCC 
    ATCATTTTGC ATGAGAATAA TGTTTCTCGA TCTTTGGGCA TGAATGCATC AGTGACAATT
    GAAGGAACAT CTGCTGTGTA CAAACTCCCA ATTGCACCAT TAATTATGGG GTCACATCCA
    GTTGACAATA AATGGACCCC TTCCTTCTCC TCAATCACCA GTGCCAACAG TGTTGATCTT
    CCTGCCTGTT TCTTCTTGAA ATTTCCCCAG CCAATCCCAG TATCTAGAGC ATTTGTTCAG
    AAACTGCAGA ACTGCACAGG AATTCCATTG TTTGAAACTC AACCAACTTA TGCACCCCTG
    TATGAACTGA TCACTCAGTT TGAGCTATCA AAGGACCCTG ACCCCATACC TTTGAATCAC
    AACATGAGAT TTTATGCTGC TCTTCCTGGT CAGCAGCACT GCTATTTCCT CAACAAGGAT
    GCTCCTCTTC CAGATGGCCG AAGTCTACAG GGAACCCTTG TTAGCAAAAT CACCTTTCAG
    CACCCTGGCC GAGTTCCTCT TATCCTAAAT CTGATCAGAC ACCAAGTGGC CTATAACACC
    CTCATTGGAA GCTGTGTCAA AAGAACTATT CTGAAAGAAG ATTCTCCTGG GCTTCTCCAA
    TTTGAAGTGT GTCCTCTCTC AGAGTCTCGT TTCAGCGTAT CTTTTCAGCA CCCTGTGAAT
    GACTCCCTGG TGTGTGTGGT AATGGATGTG CAGGACTCAA CACATGTGAG CTGTAAACTC
    TACAAAGGGC TGTCGGATGC ACTGATCTGC ACAGATGACT TCATTGCCAA AGTTGTTCAA
    AGATGTATGT CCATCCCTGT GACGATGAGG GCTATTCGGA GGAAAGCTGA AACCATTCAA
    GCCGACACCC CAGCACTGTC CCTCATTGCA GAGACAGTTG AAGACATGGT GAAAAAGAAC
    CTGCCCCCGG CTAGCAGCCC AGGGTATGGC ATGACCACAG GCAACAACCC AATGAGTGGT
    ACCACTACAC CAACCAACAC CTTTCCGGGG GGTCCCATTA CCACCTTGTT TAATATGAGC
    ATGAGCATCA AAGATCGGCA TGAGTCGGTG GGCCATGGGG AGGACTTCAG CAAGGTGTCT
    CAGAACCCAA TTCTTACCAG TTTGTTGCAA ATCACAGGGA ACGGGGGGTC TACCATTGGC
    TCGAGTCCGA CCCCTCCTCA TCACACGCCG CCACCTGTCT CTTCGATGGC CGGCAACACC
    AAGAACCACC CGATGCTCAT GAACCTTCTT AAAGATAATC CTGCCCAGGA TTTCTCAACC
    CTTTATGGAA GCAGCCCTTT AGAAAGGCAG AACTCCTCTT CCGGCTCACC CCGCATGGAA
    ATATGCTCGG GGAGCAACAA GACCAAGAAA AAGAAGTCAT CAAGATTACC ACCTGAGAAA
    CCAAAGCACC AGACTGAAGA TGACTTTCAG AGGGAGCTAT TTTCAATGGA TGTTGACTCA
    CAGAACCCTA TCTTTGATGT CAACATGACA GCTGACACGC TGGATACGCC ACACATCACT
    CCAGCTCCAA GCCAGTGTAG CACTCCCCCA ACAACTTACC CACAACCAGT ACCTCACCCC
    CAACCCAGTA TTCAAAGGAT GGTCCGACTA TCCAGTTCAG ACAGCATTGG CCCAGATGTA
    ACTGACATCC TTTCAGACAT TGCAGAAGAA GCTTCTAAAC TTCCCAGCAC TAGTGATGAT
    TGCCCAGCCA TTGGCACCCC TCTTCGAGAT TCTTCAAGCT CTGGGCATTC TCAGAGTACC
    CTGTTTGACT CTGATGTCTT TCAAACTAAC AATAATGAAA ATCCATACAC TGATCCAGCT
    GATCTTATTG CAGATGCTGC TGGAAGCCCC AGTAGTGACT CTCCTACCAA TCATTTTTTT
    CATGATGGAG TAGATTTCAA TCCTGATTTA TTGAACAGCC AGAGCCAAAG TGGTTTTGGA
    GAAGAATATT TTGATGAAAG CAGCCAAAGT GGGGATAATG ATGATTTCAA AGGATTTGCA
    TCTCAGGCAC TAAATACTTT GGGGGTGCCA ATGCTTGGAG GTGATAATGG GGAGACCAAG
    TTTAAGGGCA ATAACCAAGC CGACACAGTT GATTTCAGTA TTATTTCAGT AGCCGGCAAA
    GCTTTAGCTC CTGCAGATCT TATGGAGCAT CACAGTGGTA GTCAGGGTCC TTTACTGACC
    ACTGGGGACT TAGGGAAAGA AAAGACTCAA AAGAGGGTAA AGGAAGGCAA TGGCACCAGT
    AATAGTACTC TCTCGGGGCC CGGATTAGAC AGCAAACCAG GGAAGCGCAG TCGGACCCCT
    TCTAATGATG GGAAAAGCAA AGATAAGCCT CCAAAGCGGA AGAAGGCAGA CACTGAGGGA
    AAGTCTCCAT CTCATAGTTC TTCTAACAGA CCTTTTACCC CACCTACCAG TACAGGTGGA
    TCTAAATCGC CAGGCAGTGC AGGAAGATCT CAGACTCCCC CAGGTGTTGC CACACCACCC
    ATTCCCAAAA TCACTATTCA GATTCCTAAG GGAACAGTGA TGGTGGGCAA GCCTTCCTCT
    CACAGTCAGT ATACCAGCAG TGGTTCTGTG TCTTCCTCAG GCAGCAAAAG CCACCATAGC
    CATTCTTCCT CCTCTTCCTC ATCTGCTTCC ACCTCAGGGA AGATGAAAAG CAGTAAATCA
    GAAGGTTCAT CAAGTTCCAA GTTAAGTAGC AGTATGTATT CTAGCCAGGG GTCTTCTGGA
    TCTAGCCAGT CCAAAAATTC ATCCCAGTCT GGGGGGAAGC CAGGCTCCTC TCCCATAACC
    AAGCATGGAC TGAGCAGTGG CTCTAGCAGC ACCAAGATGA AACCTCAAGG AAAGCCATCA
    TCACTTATGA ATCCTTCTTT AAGTAAACCA AACATATCCC CTTCTCATTC AAGGCCACCT
    GGAGGCTCTG ACAAGCTTGC CTCTCCAATG AAGCCTGTTC CTGGAACTCC TCCATCCTCT
    AAAGCCAAGT CCCCTATCAG TTCAGGTTCT GGTGGTTCTC ATATGTCTGG AACTAGTTCA
    AGCTCTGGCA TGAAGTCATC TTCAGGGTTA GGATCCTCAG GCTCGTTGTC CCAGAAAACT
    CCCCCATCAT CTAATTCCTG TACGGCATCT TCCTCCTCCT TTTCCTCAAG TGGCTCTTCC
    ATGTCATCCT CTCAGAACCA GCATGGGAGT TCTAAAGGAA AATCTCCCAG CAGAAACAAG
    AAGCCGTCCT TGACAGCTGT CATAGATAAA CTGAAGCATG GGGTTGTCAC CAGTGGCCCT
    GGGGGTGAAG ACCCACTGGA CGGCCAGATG GGGGTGAGCA CAAATTCTTC CAGCCATCCT
    ATGTCCTCCA AACATAACAT GTCAGGAGGA GAGTTTCAGG GCAAGCGTGA GAAAAGTGAT
    AAAGACAAAT CAAAGGTTTC CACCTCCGGG AGTTCAGTGG ATTCTTCTAA GAAGACCTCA
    GAGTCAAAAA ATGTGGGGAG CACAGGTGTG GCAAAAATTA TCATCAGTAA GCATGATGGA
    GGCTCCCCTA GCATTAAAGC CAAAGTGACT TTGCAGAAAC CTGGGGAAAG TAGTGGAGAA
    GGGCTTAGGC CTCAAATGGC TTCTTCTAAA AACTATGGCT CTCCACTCAT CAGTGGTTCC
    ACTCCAAAGC ATGAGCGTGG CTCTCCCAGC CATAGTAAGT CACCAGCATA TACCCCCCAG
    AATCTGGACA GTGAAAGTGA GTCAGGCTCC TCCATAGCAG AGAAATCTTA TCAGAATAGT
    CCCAGCTCAG ACGATGGTAT CCGACCACTT CCAGAATACA GCACAGAGAA ACATAAGAAG
    CACAAAAAGG AAAAGAAGAA AGTAAAAGAC AAAGATAGGG ACCGAGACCG GGACAAAGAC
    CGAGACAAGA AAAAATCTCA TAGCATCAAG CCAGAGAGTT GGTCCAAATC ACCCATCTCT
    TCAGACCAGT CCTTGTCTAT GACAAGTAAC ACAATCTTAT CTGCAGACAG ACCCTCAAGG
    CTCAGCCCAG ACTTTATGAT TGGGGAGGAA GATGATGATC TTATGGATGT GGCCCTGATT
    GGGAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016880268.1
    CDS787..4875
    Translation

    Target ORF information:

    RefSeq Version XM_017024779.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mediator complex subunit 1 (MED1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017024779.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAACCTGA AGTACTATGT CTCTCCTTCT GACCTACTGG ATGACAAGAC TGCATCTCCC 
    ATCATTTTGC ATGAGAATAA TGTTTCTCGA TCTTTGGGCA TGAATGCATC AGTGACAATT
    GAAGGAACAT CTGCTGTGTA CAAACTCCCA ATTGCACCAT TAATTATGGG GTCACATCCA
    GTTGACAATA AATGGACCCC TTCCTTCTCC TCAATCACCA GTGCCAACAG TGTTGATCTT
    CCTGCCTGTT TCTTCTTGAA ATTTCCCCAG CCAATCCCAG TATCTAGAGC ATTTGTTCAG
    AAACTGCAGA ACTGCACAGG AATTCCATTG TTTGAAACTC AACCAACTTA TGCACCCCTG
    TATGAACTGA TCACTCAGTT TGAGCTATCA AAGGACCCTG ACCCCATACC TTTGAATCAC
    AACATGAGAT TTTATGCTGC TCTTCCTGGT CAGCAGCACT GCTATTTCCT CAACAAGGAT
    GCTCCTCTTC CAGATGGCCG AAGTCTACAG GGAACCCTTG TTAGCAAAAT CACCTTTCAG
    CACCCTGGCC GAGTTCCTCT TATCCTAAAT CTGATCAGAC ACCAAGTGGC CTATAACACC
    CTCATTGGAA GCTGTGTCAA AAGAACTATT CTGAAAGAAG ATTCTCCTGG GCTTCTCCAA
    TTTGAAGTGT GTCCTCTCTC AGAGTCTCGT TTCAGCGTAT CTTTTCAGCA CCCTGTGAAT
    GACTCCCTGG TGTGTGTGGT AATGGATGTG CAGGACTCAA CACATGTGAG CTGTAAACTC
    TACAAAGGGC TGTCGGATGC ACTGATCTGC ACAGATGACT TCATTGCCAA AGTTGTTCAA
    AGATGTATGT CCATCCCTGT GACGATGAGG GCTATTCGGA GGAAAGCTGA AACCATTCAA
    GCCGACACCC CAGCACTGTC CCTCATTGCA GAGACAGTTG AAGACATGGT GAAAAAGAAC
    CTGCCCCCGG CTAGCAGCCC AGGGTATGGC ATGACCACAG GCAACAACCC AATGAGTGGT
    ACCACTACAC CAACCAACAC CTTTCCGGGG GGTCCCATTA CCACCTTGTT TAATATGAGC
    ATGAGCATCA AAGATCGGCA TGAGTCGGTG GGCCATGGGG AGGACTTCAG CAAGGTGTCT
    CAGAACCCAA TTCTTACCAG TTTGTTGCAA ATCACAGGGA ACGGGGGGTC TACCATTGGC
    TCGAGTCCGA CCCCTCCTCA TCACACGCCG CCACCTGTCT CTTCGATGGC CGGCAACACC
    AAGAACCACC CGATGCTCAT GAACCTTCTT AAAGATAATC CTGCCCAGGA TTTCTCAACC
    CTTTATGGAA GCAGCCCTTT AGAAAGGCAG AACTCCTCTT CCGGCTCACC CCGCATGGAA
    ATATGCTCGG GGAGCAACAA GACCAAGAAA AAGAAGTCAT CAAGATTACC ACCTGAGAAA
    CCAAAGCACC AGACTGAAGA TGACTTTCAG AGGGAGCTAT TTTCAATGGA TGTTGACTCA
    CAGAACCCTA TCTTTGATGT CAACATGACA GCTGACACGC TGGATACGCC ACACATCACT
    CCAGCTCCAA GCCAGTGTAG CACTCCCCCA ACAACTTACC CACAACCAGT ACCTCACCCC
    CAACCCAGTA TTCAAAGGAT GGTCCGACTA TCCAGTTCAG ACAGCATTGG CCCAGATGTA
    ACTGACATCC TTTCAGACAT TGCAGAAGAA GCTTCTAAAC TTCCCAGCAC TAGTGATGAT
    TGCCCAGCCA TTGGCACCCC TCTTCGAGAT TCTTCAAGCT CTGGGCATTC TCAGAGTACC
    CTGTTTGACT CTGATGTCTT TCAAACTAAC AATAATGAAA ATCCATACAC TGATCCAGCT
    GATCTTATTG CAGATGCTGC TGGAAGCCCC AGTAGTGACT CTCCTACCAA TCATTTTTTT
    CATGATGGAG TAGATTTCAA TCCTGATTTA TTGAACAGCC AGAGCCAAAG TGGTTTTGGA
    GAAGAATATT TTGATGAAAG CAGCCAAAGT GGGGATAATG ATGATTTCAA AGGATTTGCA
    TCTCAGGCAC TAAATACTTT GGGGGTGCCA ATGCTTGGAG GTGATAATGG GGAGACCAAG
    TTTAAGGGCA ATAACCAAGC CGACACAGTT GATTTCAGTA TTATTTCAGT AGCCGGCAAA
    GCTTTAGCTC CTGCAGATCT TATGGAGCAT CACAGTGGTA GTCAGGGTCC TTTACTGACC
    ACTGGGGACT TAGGGAAAGA AAAGACTCAA AAGAGGGTAA AGGAAGGCAA TGGCACCAGT
    AATAGTACTC TCTCGGGGCC CGGATTAGAC AGCAAACCAG GGAAGCGCAG TCGGACCCCT
    TCTAATGATG GGAAAAGCAA AGATAAGCCT CCAAAGCGGA AGAAGGCAGA CACTGAGGGA
    AAGTCTCCAT CTCATAGTTC TTCTAACAGA CCTTTTACCC CACCTACCAG TACAGGTGGA
    TCTAAATCGC CAGGCAGTGC AGGAAGATCT CAGACTCCCC CAGGTGTTGC CACACCACCC
    ATTCCCAAAA TCACTATTCA GATTCCTAAG GGAACAGTGA TGGTGGGCAA GCCTTCCTCT
    CACAGTCAGT ATACCAGCAG TGGTTCTGTG TCTTCCTCAG GCAGCAAAAG CCACCATAGC
    CATTCTTCCT CCTCTTCCTC ATCTGCTTCC ACCTCAGGGA AGATGAAAAG CAGTAAATCA
    GAAGGTTCAT CAAGTTCCAA GTTAAGTAGC AGTATGTATT CTAGCCAGGG GTCTTCTGGA
    TCTAGCCAGT CCAAAAATTC ATCCCAGTCT GGGGGGAAGC CAGGCTCCTC TCCCATAACC
    AAGCATGGAC TGAGCAGTGG CTCTAGCAGC ACCAAGATGA AACCTCAAGG AAAGCCATCA
    TCACTTATGA ATCCTTCTTT AAGTAAACCA AACATATCCC CTTCTCATTC AAGGCCACCT
    GGAGGCTCTG ACAAGCTTGC CTCTCCAATG AAGCCTGTTC CTGGAACTCC TCCATCCTCT
    AAAGCCAAGT CCCCTATCAG TTCAGGTTCT GGTGGTTCTC ATATGTCTGG AACTAGTTCA
    AGCTCTGGCA TGAAGTCATC TTCAGGGTTA GGATCCTCAG GCTCGTTGTC CCAGAAAACT
    CCCCCATCAT CTAATTCCTG TACGGCATCT TCCTCCTCCT TTTCCTCAAG TGGCTCTTCC
    ATGTCATCCT CTCAGAACCA GCATGGGAGT TCTAAAGGAA AATCTCCCAG CAGAAACAAG
    AAGCCGTCCT TGACAGCTGT CATAGATAAA CTGAAGCATG GGGTTGTCAC CAGTGGCCCT
    GGGGGTGAAG ACCCACTGGA CGGCCAGATG GGGGTGAGCA CAAATTCTTC CAGCCATCCT
    ATGTCCTCCA AACATAACAT GTCAGGAGGA GAGTTTCAGG GCAAGCGTGA GAAAAGTGAT
    AAAGACAAAT CAAAGGTTTC CACCTCCGGG AGTTCAGTGG ATTCTTCTAA GAAGACCTCA
    GAGTCAAAAA ATGTGGGGAG CACAGGTGTG GCAAAAATTA TCATCAGTAA GCATGATGGA
    GGCTCCCCTA GCATTAAAGC CAAAGTGACT TTGCAGAAAC CTGGGGAAAG TAGTGGAGAA
    GGGCTTAGGC CTCAAATGGC TTCTTCTAAA AACTATGGCT CTCCACTCAT CAGTGGTTCC
    ACTCCAAAGC ATGAGCGTGG CTCTCCCAGC CATAGTAAGT CACCAGCATA TACCCCCCAG
    AATCTGGACA GTGAAAGTGA GTCAGGCTCC TCCATAGCAG AGAAATCTTA TCAGAATAGT
    CCCAGCTCAG ACGATGGTAT CCGACCACTT CCAGAATACA GCACAGAGAA ACATAAGAAG
    CACAAAAAGG AAAAGAAGAA AGTAAAAGAC AAAGATAGGG ACCGAGACCG GGACAAAGAC
    CGAGACAAGA AAAAATCTCA TAGCATCAAG CCAGAGAGTT GGTCCAAATC ACCCATCTCT
    TCAGACCAGT CCTTGTCTAT GACAAGTAAC ACAATCTTAT CTGCAGACAG ACCCTCAAGG
    CTCAGCCCAG ACTTTATGAT TGGGGAGGAA GATGATGATC TTATGGATGT GGCCCTGATT
    GGGAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu50484
    Clone ID Related Accession (Same CDS sequence) XM_005257465.3
    Accession Version XM_005257465.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4230bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mediator of RNA polymerase II transcription subunit 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257465.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTACTTGG CTCTCCAATC CTTAGAACAA GATCTTTCTA AAATGGCAAT TATGTACTGG 
    AAAGCAACTA ATGCTGGTCC CTTGGATAAG ATTCTTCATG GAAGTGTTGG CTATCTCACA
    CCAAGGAGTG GGGGTCATTT AATGAACCTG AAGTACTATG TCTCTCCTTC TGACCTACTG
    GATGACAAGA CTGCATCTCC CATCATTTTG CATGAGAATA ATGTTTCTCG ATCTTTGGGC
    ATGAATGCAT CAGTGACAAT TGAAGGAACA TCTGCTGTGT ACAAACTCCC AATTGCACCA
    TTAATTATGG GGTCACATCC AGTTGACAAT AAATGGACCC CTTCCTTCTC CTCAATCACC
    AGTGCCAACA GTGTTGATCT TCCTGCCTGT TTCTTCTTGA AATTTCCCCA GCCAATCCCA
    GTATCTAGAG CATTTGTTCA GAAACTGCAG AACTGCACAG GAATTCCATT GTTTGAAACT
    CAACCAACTT ATGCACCCCT GTATGAACTG ATCACTCAGT TTGAGCTATC AAAGGACCCT
    GACCCCATAC CTTTGAATCA CAACATGAGA TTTTATGCTG CTCTTCCTGG TCAGCAGCAC
    TGCTATTTCC TCAACAAGGA TGCTCCTCTT CCAGATGGCC GAAGTCTACA GGGAACCCTT
    GTTAGCAAAA TCACCTTTCA GCACCCTGGC CGAGTTCCTC TTATCCTAAA TCTGATCAGA
    CACCAAGTGG CCTATAACAC CCTCATTGGA AGCTGTGTCA AAAGAACTAT TCTGAAAGAA
    GATTCTCCTG GGCTTCTCCA ATTTGAAGTG TGTCCTCTCT CAGAGTCTCG TTTCAGCGTA
    TCTTTTCAGC ACCCTGTGAA TGACTCCCTG GTGTGTGTGG TAATGGATGT GCAGGACTCA
    ACACATGTGA GCTGTAAACT CTACAAAGGG CTGTCGGATG CACTGATCTG CACAGATGAC
    TTCATTGCCA AAGTTGTTCA AAGATGTATG TCCATCCCTG TGACGATGAG GGCTATTCGG
    AGGAAAGCTG AAACCATTCA AGCCGACACC CCAGCACTGT CCCTCATTGC AGAGACAGTT
    GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA
    GGCAACAACC CAATGAGTGG TACCACTACA CCAACCAACA CCTTTCCGGG GGGTCCCATT
    ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG
    GAGGACTTCA GCAAGGTGTC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG
    AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC
    TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAT
    CCTGCCCAGG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCCTCT
    TCCGGCTCAC CCCGCATGGA AATATGCTCG GGGAGCAACA AGACCAAGAA AAAGAAGTCA
    TCAAGATTAC CACCTGAGAA ACCAAAGCAC CAGACTGAAG ATGACTTTCA GAGGGAGCTA
    TTTTCAATGG ATGTTGACTC ACAGAACCCT ATCTTTGATG TCAACATGAC AGCTGACACG
    CTGGATACGC CACACATCAC TCCAGCTCCA AGCCAGTGTA GCACTCCCCC AACAACTTAC
    CCACAACCAG TACCTCACCC CCAACCCAGT ATTCAAAGGA TGGTCCGACT ATCCAGTTCA
    GACAGCATTG GCCCAGATGT AACTGACATC CTTTCAGACA TTGCAGAAGA AGCTTCTAAA
    CTTCCCAGCA CTAGTGATGA TTGCCCAGCC ATTGGCACCC CTCTTCGAGA TTCTTCAAGC
    TCTGGGCATT CTCAGAGTAC CCTGTTTGAC TCTGATGTCT TTCAAACTAA CAATAATGAA
    AATCCATACA CTGATCCAGC TGATCTTATT GCAGATGCTG CTGGAAGCCC CAGTAGTGAC
    TCTCCTACCA ATCATTTTTT TCATGATGGA GTAGATTTCA ATCCTGATTT ATTGAACAGC
    CAGAGCCAAA GTGGTTTTGG AGAAGAATAT TTTGATGAAA GCAGCCAAAG TGGGGATAAT
    GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGGTGCC AATGCTTGGA
    GGTGATAATG GGGAGACCAA GTTTAAGGGC AATAACCAAG CCGACACAGT TGATTTCAGT
    ATTATTTCAG TAGCCGGCAA AGCTTTAGCT CCTGCAGATC TTATGGAGCA TCACAGTGGT
    AGTCAGGGTC CTTTACTGAC CACTGGGGAC TTAGGGAAAG AAAAGACTCA AAAGAGGGTA
    AAGGAAGGCA ATGGCACCAG TAATAGTACT CTCTCGGGGC CCGGATTAGA CAGCAAACCA
    GGGAAGCGCA GTCGGACCCC TTCTAATGAT GGGAAAAGCA AAGATAAGCC TCCAAAGCGG
    AAGAAGGCAG ACACTGAGGG AAAGTCTCCA TCTCATAGTT CTTCTAACAG ACCTTTTACC
    CCACCTACCA GTACAGGTGG ATCTAAATCG CCAGGCAGTG CAGGAAGATC TCAGACTCCC
    CCAGGTGTTG CCACACCACC CATTCCCAAA ATCACTATTC AGATTCCTAA GGGAACAGTG
    ATGGTGGGCA AGCCTTCCTC TCACAGTCAG TATACCAGCA GTGGTTCTGT GTCTTCCTCA
    GGCAGCAAAA GCCACCATAG CCATTCTTCC TCCTCTTCCT CATCTGCTTC CACCTCAGGG
    AAGATGAAAA GCAGTAAATC AGAAGGTTCA TCAAGTTCCA AGTTAAGTAG CAGTATGTAT
    TCTAGCCAGG GGTCTTCTGG ATCTAGCCAG TCCAAAAATT CATCCCAGTC TGGGGGGAAG
    CCAGGCTCCT CTCCCATAAC CAAGCATGGA CTGAGCAGTG GCTCTAGCAG CACCAAGATG
    AAACCTCAAG GAAAGCCATC ATCACTTATG AATCCTTCTT TAAGTAAACC AAACATATCC
    CCTTCTCATT CAAGGCCACC TGGAGGCTCT GACAAGCTTG CCTCTCCAAT GAAGCCTGTT
    CCTGGAACTC CTCCATCCTC TAAAGCCAAG TCCCCTATCA GTTCAGGTTC TGGTGGTTCT
    CATATGTCTG GAACTAGTTC AAGCTCTGGC ATGAAGTCAT CTTCAGGGTT AGGATCCTCA
    GGCTCGTTGT CCCAGAAAAC TCCCCCATCA TCTAATTCCT GTACGGCATC TTCCTCCTCC
    TTTTCCTCAA GTGGCTCTTC CATGTCATCC TCTCAGAACC AGCATGGGAG TTCTAAAGGA
    AAATCTCCCA GCAGAAACAA GAAGCCGTCC TTGACAGCTG TCATAGATAA ACTGAAGCAT
    GGGGTTGTCA CCAGTGGCCC TGGGGGTGAA GACCCACTGG ACGGCCAGAT GGGGGTGAGC
    ACAAATTCTT CCAGCCATCC TATGTCCTCC AAACATAACA TGTCAGGAGG AGAGTTTCAG
    GGCAAGCGTG AGAAAAGTGA TAAAGACAAA TCAAAGGTTT CCACCTCCGG GAGTTCAGTG
    GATTCTTCTA AGAAGACCTC AGAGTCAAAA AATGTGGGGA GCACAGGTGT GGCAAAAATT
    ATCATCAGTA AGCATGATGG AGGCTCCCCT AGCATTAAAG CCAAAGTGAC TTTGCAGAAA
    CCTGGGGAAA GTAGTGGAGA AGGGCTTAGG CCTCAAATGG CTTCTTCTAA AAACTATGGC
    TCTCCACTCA TCAGTGGTTC CACTCCAAAG CATGAGCGTG GCTCTCCCAG CCATAGTAAG
    TCACCAGCAT ATACCCCCCA GAATCTGGAC AGTGAAAGTG AGTCAGGCTC CTCCATAGCA
    GAGAAATCTT ATCAGAATAG TCCCAGCTCA GACGATGGTA TCCGACCACT TCCAGAATAC
    AGCACAGAGA AACATAAGAA GCACAAAAAG GAAAAGAAGA AAGTAAAAGA CAAAGATAGG
    GACCGAGACC GGGACAAAGA CCGAGACAAG AAAAAATCTC ATAGCATCAA GCCAGAGAGT
    TGGTCCAAAT CACCCATCTC TTCAGACCAG TCCTTGTCTA TGACAAGTAA CACAATCTTA
    TCTGCAGACA GACCCTCAAG GCTCAGCCCA GACTTTATGA TTGGGGAGGA AGATGATGAT
    CTTATGGATG TGGCCCTGAT TGGGAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005257522.1
    CDS603..4832
    Translation

    Target ORF information:

    RefSeq Version XM_005257465.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mediator complex subunit 1 (MED1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005257465.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTACTTGG CTCTCCAATC CTTAGAACAA GATCTTTCTA AAATGGCAAT TATGTACTGG 
    AAAGCAACTA ATGCTGGTCC CTTGGATAAG ATTCTTCATG GAAGTGTTGG CTATCTCACA
    CCAAGGAGTG GGGGTCATTT AATGAACCTG AAGTACTATG TCTCTCCTTC TGACCTACTG
    GATGACAAGA CTGCATCTCC CATCATTTTG CATGAGAATA ATGTTTCTCG ATCTTTGGGC
    ATGAATGCAT CAGTGACAAT TGAAGGAACA TCTGCTGTGT ACAAACTCCC AATTGCACCA
    TTAATTATGG GGTCACATCC AGTTGACAAT AAATGGACCC CTTCCTTCTC CTCAATCACC
    AGTGCCAACA GTGTTGATCT TCCTGCCTGT TTCTTCTTGA AATTTCCCCA GCCAATCCCA
    GTATCTAGAG CATTTGTTCA GAAACTGCAG AACTGCACAG GAATTCCATT GTTTGAAACT
    CAACCAACTT ATGCACCCCT GTATGAACTG ATCACTCAGT TTGAGCTATC AAAGGACCCT
    GACCCCATAC CTTTGAATCA CAACATGAGA TTTTATGCTG CTCTTCCTGG TCAGCAGCAC
    TGCTATTTCC TCAACAAGGA TGCTCCTCTT CCAGATGGCC GAAGTCTACA GGGAACCCTT
    GTTAGCAAAA TCACCTTTCA GCACCCTGGC CGAGTTCCTC TTATCCTAAA TCTGATCAGA
    CACCAAGTGG CCTATAACAC CCTCATTGGA AGCTGTGTCA AAAGAACTAT TCTGAAAGAA
    GATTCTCCTG GGCTTCTCCA ATTTGAAGTG TGTCCTCTCT CAGAGTCTCG TTTCAGCGTA
    TCTTTTCAGC ACCCTGTGAA TGACTCCCTG GTGTGTGTGG TAATGGATGT GCAGGACTCA
    ACACATGTGA GCTGTAAACT CTACAAAGGG CTGTCGGATG CACTGATCTG CACAGATGAC
    TTCATTGCCA AAGTTGTTCA AAGATGTATG TCCATCCCTG TGACGATGAG GGCTATTCGG
    AGGAAAGCTG AAACCATTCA AGCCGACACC CCAGCACTGT CCCTCATTGC AGAGACAGTT
    GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA
    GGCAACAACC CAATGAGTGG TACCACTACA CCAACCAACA CCTTTCCGGG GGGTCCCATT
    ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG
    GAGGACTTCA GCAAGGTGTC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG
    AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC
    TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAT
    CCTGCCCAGG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCCTCT
    TCCGGCTCAC CCCGCATGGA AATATGCTCG GGGAGCAACA AGACCAAGAA AAAGAAGTCA
    TCAAGATTAC CACCTGAGAA ACCAAAGCAC CAGACTGAAG ATGACTTTCA GAGGGAGCTA
    TTTTCAATGG ATGTTGACTC ACAGAACCCT ATCTTTGATG TCAACATGAC AGCTGACACG
    CTGGATACGC CACACATCAC TCCAGCTCCA AGCCAGTGTA GCACTCCCCC AACAACTTAC
    CCACAACCAG TACCTCACCC CCAACCCAGT ATTCAAAGGA TGGTCCGACT ATCCAGTTCA
    GACAGCATTG GCCCAGATGT AACTGACATC CTTTCAGACA TTGCAGAAGA AGCTTCTAAA
    CTTCCCAGCA CTAGTGATGA TTGCCCAGCC ATTGGCACCC CTCTTCGAGA TTCTTCAAGC
    TCTGGGCATT CTCAGAGTAC CCTGTTTGAC TCTGATGTCT TTCAAACTAA CAATAATGAA
    AATCCATACA CTGATCCAGC TGATCTTATT GCAGATGCTG CTGGAAGCCC CAGTAGTGAC
    TCTCCTACCA ATCATTTTTT TCATGATGGA GTAGATTTCA ATCCTGATTT ATTGAACAGC
    CAGAGCCAAA GTGGTTTTGG AGAAGAATAT TTTGATGAAA GCAGCCAAAG TGGGGATAAT
    GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGGTGCC AATGCTTGGA
    GGTGATAATG GGGAGACCAA GTTTAAGGGC AATAACCAAG CCGACACAGT TGATTTCAGT
    ATTATTTCAG TAGCCGGCAA AGCTTTAGCT CCTGCAGATC TTATGGAGCA TCACAGTGGT
    AGTCAGGGTC CTTTACTGAC CACTGGGGAC TTAGGGAAAG AAAAGACTCA AAAGAGGGTA
    AAGGAAGGCA ATGGCACCAG TAATAGTACT CTCTCGGGGC CCGGATTAGA CAGCAAACCA
    GGGAAGCGCA GTCGGACCCC TTCTAATGAT GGGAAAAGCA AAGATAAGCC TCCAAAGCGG
    AAGAAGGCAG ACACTGAGGG AAAGTCTCCA TCTCATAGTT CTTCTAACAG ACCTTTTACC
    CCACCTACCA GTACAGGTGG ATCTAAATCG CCAGGCAGTG CAGGAAGATC TCAGACTCCC
    CCAGGTGTTG CCACACCACC CATTCCCAAA ATCACTATTC AGATTCCTAA GGGAACAGTG
    ATGGTGGGCA AGCCTTCCTC TCACAGTCAG TATACCAGCA GTGGTTCTGT GTCTTCCTCA
    GGCAGCAAAA GCCACCATAG CCATTCTTCC TCCTCTTCCT CATCTGCTTC CACCTCAGGG
    AAGATGAAAA GCAGTAAATC AGAAGGTTCA TCAAGTTCCA AGTTAAGTAG CAGTATGTAT
    TCTAGCCAGG GGTCTTCTGG ATCTAGCCAG TCCAAAAATT CATCCCAGTC TGGGGGGAAG
    CCAGGCTCCT CTCCCATAAC CAAGCATGGA CTGAGCAGTG GCTCTAGCAG CACCAAGATG
    AAACCTCAAG GAAAGCCATC ATCACTTATG AATCCTTCTT TAAGTAAACC AAACATATCC
    CCTTCTCATT CAAGGCCACC TGGAGGCTCT GACAAGCTTG CCTCTCCAAT GAAGCCTGTT
    CCTGGAACTC CTCCATCCTC TAAAGCCAAG TCCCCTATCA GTTCAGGTTC TGGTGGTTCT
    CATATGTCTG GAACTAGTTC AAGCTCTGGC ATGAAGTCAT CTTCAGGGTT AGGATCCTCA
    GGCTCGTTGT CCCAGAAAAC TCCCCCATCA TCTAATTCCT GTACGGCATC TTCCTCCTCC
    TTTTCCTCAA GTGGCTCTTC CATGTCATCC TCTCAGAACC AGCATGGGAG TTCTAAAGGA
    AAATCTCCCA GCAGAAACAA GAAGCCGTCC TTGACAGCTG TCATAGATAA ACTGAAGCAT
    GGGGTTGTCA CCAGTGGCCC TGGGGGTGAA GACCCACTGG ACGGCCAGAT GGGGGTGAGC
    ACAAATTCTT CCAGCCATCC TATGTCCTCC AAACATAACA TGTCAGGAGG AGAGTTTCAG
    GGCAAGCGTG AGAAAAGTGA TAAAGACAAA TCAAAGGTTT CCACCTCCGG GAGTTCAGTG
    GATTCTTCTA AGAAGACCTC AGAGTCAAAA AATGTGGGGA GCACAGGTGT GGCAAAAATT
    ATCATCAGTA AGCATGATGG AGGCTCCCCT AGCATTAAAG CCAAAGTGAC TTTGCAGAAA
    CCTGGGGAAA GTAGTGGAGA AGGGCTTAGG CCTCAAATGG CTTCTTCTAA AAACTATGGC
    TCTCCACTCA TCAGTGGTTC CACTCCAAAG CATGAGCGTG GCTCTCCCAG CCATAGTAAG
    TCACCAGCAT ATACCCCCCA GAATCTGGAC AGTGAAAGTG AGTCAGGCTC CTCCATAGCA
    GAGAAATCTT ATCAGAATAG TCCCAGCTCA GACGATGGTA TCCGACCACT TCCAGAATAC
    AGCACAGAGA AACATAAGAA GCACAAAAAG GAAAAGAAGA AAGTAAAAGA CAAAGATAGG
    GACCGAGACC GGGACAAAGA CCGAGACAAG AAAAAATCTC ATAGCATCAA GCCAGAGAGT
    TGGTCCAAAT CACCCATCTC TTCAGACCAG TCCTTGTCTA TGACAAGTAA CACAATCTTA
    TCTGCAGACA GACCCTCAAG GCTCAGCCCA GACTTTATGA TTGGGGAGGA AGATGATGAT
    CTTATGGATG TGGCCCTGAT TGGGAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.