MED1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED1
Entrez Gene ID	480533
Full Name	mediator complex subunit 1
Synonyms	PPARBP
General protein information	Preferred Names mediator complex subunit 1 Names mediator of RNA polymerase II transcription subunit 1 PPAR binding protein
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_858916.4	XP_864009.2	mediator of RNA polymerase II transcription subunit 1 isoform X1
XM_858916.5	XP_864009.2	mediator of RNA polymerase II transcription subunit 1 isoform X1
XM_014116603.2	XP_013972078.1	mediator of RNA polymerase II transcription subunit 1 isoform X2
XM_022423023.1	XP_022278731.1	mediator of RNA polymerase II transcription subunit 1 isoform X2
XM_014116603.1	XP_013972078.1	mediator of RNA polymerase II transcription subunit 1 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MED1	XP_001172406.1
Canis lupus familiaris (dog)	MED1	XP_864009.2
Rattus norvegicus (Norway rat)	Med1	NP_001127833.1
Gallus gallus (chicken)	MED1	XP_418125.3
Bos taurus (cattle)	MED1	NP_001192805.1
Mus musculus (house mouse)	Med1	NP_001073587.1
Homo sapiens (human)	MED1	NP_004765.2
Xenopus tropicalis (tropical clawed frog)	med1	NP_001090727.1

Gene Ontology

The following MED1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe38367	XM_022423023.1 Latest version!	Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe38367	XM_014116603.2 Latest version!	Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe11780	XM_858916.5 Latest version!	Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OCe11780	XM_858916.4	Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe38367	XM_014116603.1	Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED1 ( XM_014116603.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013972078.1 Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe38367
Clone ID Related Accession (Same CDS sequence)	XM_014116603.2 , XM_022423023.1 , XM_014116603.1
Accession Version	XM_014116603.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mediator of RNA polymerase II transcription subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014116603.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTGG CTCTCCAATC CTTAGAACAG GATCTATCTA AAATGGCAGT TATGTATTGG 
AAAGCAACCA ATGCTGGTCC CTTGGATAAG ATTCTTCATG GAAGTGTTGG CTATCTTACT 
CCAAGAAGTG GGGGTCATTT AATGAACTTG AAGTACTATG CCTCTCCTTC TGACCTGCTG 
GATGATAAGA CTACATCTCC CATCATTTTG CATGAGAATA ATGTTCCTCG ATCATTGGGC 
ATGAACGCAT CAGTGACAAT TGAAGGAACA TCTGCTATGT ATAAACTCCC AATTGCACCA 
TTAATTATGG GGTCACATCC AGTTGACAAC AAATGGACCC CCTCCTTCTC CTCAATCACC 
AGTGCCAACA GTGTTGATCT TCCTGCCTGT TTCTTCTTGA AATTTCCCCA ACCAATCCCA 
GTATCTAGAG CATTTGTTCA GAAACTTCAG AACTGCACAG GAATTCCATT GTTTGAAACT 
CAACCAACTT ATGTACCCCT CTATGAACTG ATCACTCAGT TTGAGCTGTC AAAGGACCCT 
GACCCCATAC CTTTGAATCA CAACATGAGA TTTTATGCTG CTCTTCCAGG TCAGCAGCAC 
TGCTATTTCC TCAACAAGGA TGCTCCTCTT CCAGATGGCC GAAGTCTACA GGGAACCCTA 
GTTAGCAAAA TCACCTTTCA GCACCCTGGC CGAGTTCCTC TTATCCTAAA TCTGATCAGA 
CATCAAGTGG CCTATAACAC CCTAATAGGA AGTTGTGTCA AAAGAACTAT TCTGAAAGAA 
GATTCTCCTG GGCTCCTCCA ATTTGAAGTG TGTCCTCTCT CAGAGTCCCG TTTTAGCGTA 
TCTTTTCAGC ACCCTGTGAA TGACTCCCTG GTGTGTGTGG TAATGGATGT GCAGGACTCA 
ACACATGTGA GCTGTAAACT GTACAAGGGG CTGTCAGATG CACTCATCTG CACAGATGAC 
TTCATTGCCA AAGTTGTTCA AAGATGTATG TCCATCCCTG TGACGATGAG GGCTATTCGG 
AGGAAAGCTG AAACCATTCA GGCCGACACC CCAGCACTGT CCCTCATTGC AGAGACAGTT 
GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA 
GGCAACAACC CAATGAGTGG TACCACTACA CCAACCAACA CCTTTCCGGG GGGTCCCATT 
ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG 
GAGGACTTCA GCAAGGTGTC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG 
AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC 
TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAC 
CCTGCCCAGG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCTTCT 
TCCGGCTCAC CCCGGATGGA AATATGCCCG GGAAGCAACA AGACAAAGAA GAAGAAGTCA 
TCAAGATTAC CACCTGACAA ACCCAAGCAC CAGACTGAAG ATGACTTTCA GAGGGAGCTA 
TTTTCAATGG ATGTTGACTC ACAGAACCCT ATCTTTGATG TCAACATGAC AGCAGACACG 
CTGGATACGC CACACATCAC TCCAGCTCCA AGCCAGTGTA GTACTCCCCC AACAACTTAC 
CCGCAACCAG TACCTCACCC CCAACCCAGT ATTCAAAGGA TGGTCCGACT ATCCAGTTCA 
GACAGCATTG GCCCAGATGT AACTGATATC CTTTCAGACA TTGCAGAAGA AGCCTCTAAG 
CTTCCCAGCA CTAATGACGA TTGTCCACCC ATTGGCACCC CTGTTCGAGA TTCTTCAAGC 
TCTGGGCATT CTCAGAGTGC TCTCTTTGAC CCTGATGTCT TTCAAACCAA TAATAATGAA 
AATCCATACA CAGATCCAGC TGACCTAATT GCAGATGCTG CTGGTAGCCC TAGTAGTGAC 
TCTCCTACAA ATCATTTTTT TCCTGATGGA GTAGATTTTA ATCCTGATTT GTTGAACAGC 
CAGAGCCAAA GTGGTTTTGG AGAAGAATAT TTTGATGAAA GCAGCCAAAG CGGAGATAAT 
GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGGTGCC AATGCTTGGA 
GGTGATAATG GGGAGACTAA GTTTAAAGGC AATAGCCAAG CTGACACAGT TGATTTCAGT 
ATTATAGCAG TGGCTGGTAA GGCTTTAGGT CCTGCAGATC TTATGGAGCA TCACAGTGGT 
AGCCAGAGTC CTTTATTGAC TACTGGGGAC TTAGGGAAAG AAAAGACTCA AAAACGAGTA 
AAGGAAGGCA ATGGCACCAG TAACAGTAGT CTGTCAGGGC CAGGATTAGA TAGCAAACCA 
GGGAAGCGTA GTCGGACCCC TTCTAATGAT GGCAAAAGCA AAGATAAGCC TCCAAAGCGG 
AAGAAGGCAG ACACTGAAGG AAAGTCTCCA TCTCACAGTT CTTCTAACCG ACCTTTCACT 
CCACCTACCA GTACGGGTGG ATCCAAATCA CCAGGCAGTT CAGGAAGATC ACAGACTCCC 
CCAGGTGTTG CCACACCACC TATTCCCAAA ATCACTATTC AGATTCCTAA AGGTACCGTG 
ATGGTGGGCA AGCCCTCATC CCACAGTCAG TATACCAGCA GTGGTTCTGT GTCTTCCTCA 
GGCAGTAAAA GCCACCATAG CCATTCTTCC TCCTCTTCTT CCTCCTCTTC TGCTTCCAAC 
TCAGGCAAGA TGAAAAGCAG TAAATCAGAA GGTTCATCAA GTTCCAAGTT AAGTAGCAGT 
GTATATTCTA GCCAAGGGTC TTCTGGATCT AGTCAGTCCA AAAATTCATC CCAGTCTGGG 
GGGAAGCCAG GCTCCTCTCC CATTACCAAG CATGGACTGA GCAGTGGCTC CGGCAGCACC 
AAGATGAAAC CTCAAGGGAA GCCATCATCA CTTATGAATC CTTCTTTAAG TAAACCAAAC 
ATATCCCCTT CTCATTCAAG GCCACCTGGA GGCTCTGATA AGCTTGCCTC TCCAATGAAG 
CCTGTTCCTG GGACTCCCCC ATCTTCTAAA GCCAAGTCCC CCATCAGTTC AGGTTCTGGT 
GGTTCTCATA TGTCTGGAAG TAGTTCAAGC ACTGGCATGA AGTCATCTTC AGGGTTAGGA 
TCTTCAGGCT CATTGTCTCA GAAAACTCCC CCATCATCTA ATTCTTGTAC AGCATCTTCC 
TCTTCCTTTT CCTCAAGTGG CTCTTCCATG TCATCCTCTC AGAACCAGCA CGGGAGTTCC 
AAAGGGAAAT CCCCCAGCAG AAATAAGAAG CCGTCCTTAA CAGCCGTCAT AGATAAACTG 
AAGCATGGGG TTGTCACCAG TGGCCCTGGA GGTGAAGACC CGATGGATGG CCAGATGGGA 
GTGAGCACAA ATTCTTCTAG CCATCCTATG TCCTCCAAAC ATAACATGTC AGGAGGAGAG 
TTCCAGGGTA AGCGTGAGAA AAGTGATAAA GAAAAATCCA AGGTTTCCAC CTCGGGGGGT 
TCAGTGGATT CGTCTAAGAA GACCTCAGAG TCAAAAAATG TGGGGAGCAC AGGTGTGGCA 
AAAATTATCA TCAGCAAGCA TGATGGGGGT TCCCCCAGCA TTAAAGCCAA AGTGACTTTG 
CAGAAACCTG GGGAAAGTAG TGGAGAAGGG CTCAGACCTC AGATGGCCTC TTCCAAAAAC 
TATGGCTCTC CACTCATCAG TGGTTCCACT CCAAAGCATG AGCGTGGCTC TCCTAGCCAT 
AGTAAGTCAC CAGCATATAC CCCCCAGAAT CTGGACAGTG AAAGTGAGTC AGGCTCCTCC 
ATAGCAGAGA AATCTTATCA GAACAGTCCG AGCTCAGATG ATGGTATCCG ACCTCTTCCA 
GAATATAGCA CAGAAAAGCA TAAGAAGCAC AAAAAAGAAA AGAAGAAAGT AAAAGACAAA 
GATAGAGACC GGGACCGGGA CAAAGACCGG GACAAGAAAA AATCTCATAG CATCAAGCCA 
GAGAGTTGGT CTAAATCACC CATCTCTTCA GACCAGTCCT TGTCTATGAC AAGCAACACA 
ATTTTATCTA CAGACAGGCC CTCAAGGCTC AGCCCTGACT TTATGATTGG GGAGGAAGAT 
GATGATCTTA TGGATGTGGC CCTGATTGGC AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972078.1
CDS	234..4469
Translation	MYLALQSLEQDLSKMAVMYWKATNAGPLDKILHGSVGYLTPRSGGHLMNLKYYASPSDLLDDKTTSPIILHENNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYVPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEICPGSNKTKKKKSSRLPPDKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTNDDCPPIGTPVRDSSSSGHSQSALFDPDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNSQADTVDFSIIAVAGKALGPADLMEHHSGSQSPLLTTGDLGKEKTQKRVKEGNGTSNSSLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSSSASNSGKMKSSKSEGSSSSKLSSSVYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSGSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGSSSSTGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPMDGQMGVSTNSSSHPMSSKHNMSGGEFQGKREKSDKEKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSTDRPSRLSPDFMIGEEDDDLMDVALIGN

Target ORF information:

RefSeq Version	XM_014116603.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116603.2

ORF Insert Sequence:

ATGTACTTGG CTCTCCAATC CTTAGAACAG GATCTATCTA AAATGGCAGT TATGTATTGG 
AAAGCAACCA ATGCTGGTCC CTTGGATAAG ATTCTTCATG GAAGTGTTGG CTATCTTACT 
CCAAGAAGTG GGGGTCATTT AATGAACTTG AAGTACTATG CCTCTCCTTC TGACCTGCTG 
GATGATAAGA CTACATCTCC CATCATTTTG CATGAGAATA ATGTTCCTCG ATCATTGGGC 
ATGAACGCAT CAGTGACAAT TGAAGGAACA TCTGCTATGT ATAAACTCCC AATTGCACCA 
TTAATTATGG GGTCACATCC AGTTGACAAC AAATGGACCC CCTCCTTCTC CTCAATCACC 
AGTGCCAACA GTGTTGATCT TCCTGCCTGT TTCTTCTTGA AATTTCCCCA ACCAATCCCA 
GTATCTAGAG CATTTGTTCA GAAACTTCAG AACTGCACAG GAATTCCATT GTTTGAAACT 
CAACCAACTT ATGTACCCCT CTATGAACTG ATCACTCAGT TTGAGCTGTC AAAGGACCCT 
GACCCCATAC CTTTGAATCA CAACATGAGA TTTTATGCTG CTCTTCCAGG TCAGCAGCAC 
TGCTATTTCC TCAACAAGGA TGCTCCTCTT CCAGATGGCC GAAGTCTACA GGGAACCCTA 
GTTAGCAAAA TCACCTTTCA GCACCCTGGC CGAGTTCCTC TTATCCTAAA TCTGATCAGA 
CATCAAGTGG CCTATAACAC CCTAATAGGA AGTTGTGTCA AAAGAACTAT TCTGAAAGAA 
GATTCTCCTG GGCTCCTCCA ATTTGAAGTG TGTCCTCTCT CAGAGTCCCG TTTTAGCGTA 
TCTTTTCAGC ACCCTGTGAA TGACTCCCTG GTGTGTGTGG TAATGGATGT GCAGGACTCA 
ACACATGTGA GCTGTAAACT GTACAAGGGG CTGTCAGATG CACTCATCTG CACAGATGAC 
TTCATTGCCA AAGTTGTTCA AAGATGTATG TCCATCCCTG TGACGATGAG GGCTATTCGG 
AGGAAAGCTG AAACCATTCA GGCCGACACC CCAGCACTGT CCCTCATTGC AGAGACAGTT 
GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA 
GGCAACAACC CAATGAGTGG TACCACTACA CCAACCAACA CCTTTCCGGG GGGTCCCATT 
ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG 
GAGGACTTCA GCAAGGTGTC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG 
AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC 
TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAC 
CCTGCCCAGG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCTTCT 
TCCGGCTCAC CCCGGATGGA AATATGCCCG GGAAGCAACA AGACAAAGAA GAAGAAGTCA 
TCAAGATTAC CACCTGACAA ACCCAAGCAC CAGACTGAAG ATGACTTTCA GAGGGAGCTA 
TTTTCAATGG ATGTTGACTC ACAGAACCCT ATCTTTGATG TCAACATGAC AGCAGACACG 
CTGGATACGC CACACATCAC TCCAGCTCCA AGCCAGTGTA GTACTCCCCC AACAACTTAC 
CCGCAACCAG TACCTCACCC CCAACCCAGT ATTCAAAGGA TGGTCCGACT ATCCAGTTCA 
GACAGCATTG GCCCAGATGT AACTGATATC CTTTCAGACA TTGCAGAAGA AGCCTCTAAG 
CTTCCCAGCA CTAATGACGA TTGTCCACCC ATTGGCACCC CTGTTCGAGA TTCTTCAAGC 
TCTGGGCATT CTCAGAGTGC TCTCTTTGAC CCTGATGTCT TTCAAACCAA TAATAATGAA 
AATCCATACA CAGATCCAGC TGACCTAATT GCAGATGCTG CTGGTAGCCC TAGTAGTGAC 
TCTCCTACAA ATCATTTTTT TCCTGATGGA GTAGATTTTA ATCCTGATTT GTTGAACAGC 
CAGAGCCAAA GTGGTTTTGG AGAAGAATAT TTTGATGAAA GCAGCCAAAG CGGAGATAAT 
GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGGTGCC AATGCTTGGA 
GGTGATAATG GGGAGACTAA GTTTAAAGGC AATAGCCAAG CTGACACAGT TGATTTCAGT 
ATTATAGCAG TGGCTGGTAA GGCTTTAGGT CCTGCAGATC TTATGGAGCA TCACAGTGGT 
AGCCAGAGTC CTTTATTGAC TACTGGGGAC TTAGGGAAAG AAAAGACTCA AAAACGAGTA 
AAGGAAGGCA ATGGCACCAG TAACAGTAGT CTGTCAGGGC CAGGATTAGA TAGCAAACCA 
GGGAAGCGTA GTCGGACCCC TTCTAATGAT GGCAAAAGCA AAGATAAGCC TCCAAAGCGG 
AAGAAGGCAG ACACTGAAGG AAAGTCTCCA TCTCACAGTT CTTCTAACCG ACCTTTCACT 
CCACCTACCA GTACGGGTGG ATCCAAATCA CCAGGCAGTT CAGGAAGATC ACAGACTCCC 
CCAGGTGTTG CCACACCACC TATTCCCAAA ATCACTATTC AGATTCCTAA AGGTACCGTG 
ATGGTGGGCA AGCCCTCATC CCACAGTCAG TATACCAGCA GTGGTTCTGT GTCTTCCTCA 
GGCAGTAAAA GCCACCATAG CCATTCTTCC TCCTCTTCTT CCTCCTCTTC TGCTTCCAAC 
TCAGGCAAGA TGAAAAGCAG TAAATCAGAA GGTTCATCAA GTTCCAAGTT AAGTAGCAGT 
GTATATTCTA GCCAAGGGTC TTCTGGATCT AGTCAGTCCA AAAATTCATC CCAGTCTGGG 
GGGAAGCCAG GCTCCTCTCC CATTACCAAG CATGGACTGA GCAGTGGCTC CGGCAGCACC 
AAGATGAAAC CTCAAGGGAA GCCATCATCA CTTATGAATC CTTCTTTAAG TAAACCAAAC 
ATATCCCCTT CTCATTCAAG GCCACCTGGA GGCTCTGATA AGCTTGCCTC TCCAATGAAG 
CCTGTTCCTG GGACTCCCCC ATCTTCTAAA GCCAAGTCCC CCATCAGTTC AGGTTCTGGT 
GGTTCTCATA TGTCTGGAAG TAGTTCAAGC ACTGGCATGA AGTCATCTTC AGGGTTAGGA 
TCTTCAGGCT CATTGTCTCA GAAAACTCCC CCATCATCTA ATTCTTGTAC AGCATCTTCC 
TCTTCCTTTT CCTCAAGTGG CTCTTCCATG TCATCCTCTC AGAACCAGCA CGGGAGTTCC 
AAAGGGAAAT CCCCCAGCAG AAATAAGAAG CCGTCCTTAA CAGCCGTCAT AGATAAACTG 
AAGCATGGGG TTGTCACCAG TGGCCCTGGA GGTGAAGACC CGATGGATGG CCAGATGGGA 
GTGAGCACAA ATTCTTCTAG CCATCCTATG TCCTCCAAAC ATAACATGTC AGGAGGAGAG 
TTCCAGGGTA AGCGTGAGAA AAGTGATAAA GAAAAATCCA AGGTTTCCAC CTCGGGGGGT 
TCAGTGGATT CGTCTAAGAA GACCTCAGAG TCAAAAAATG TGGGGAGCAC AGGTGTGGCA 
AAAATTATCA TCAGCAAGCA TGATGGGGGT TCCCCCAGCA TTAAAGCCAA AGTGACTTTG 
CAGAAACCTG GGGAAAGTAG TGGAGAAGGG CTCAGACCTC AGATGGCCTC TTCCAAAAAC 
TATGGCTCTC CACTCATCAG TGGTTCCACT CCAAAGCATG AGCGTGGCTC TCCTAGCCAT 
AGTAAGTCAC CAGCATATAC CCCCCAGAAT CTGGACAGTG AAAGTGAGTC AGGCTCCTCC 
ATAGCAGAGA AATCTTATCA GAACAGTCCG AGCTCAGATG ATGGTATCCG ACCTCTTCCA 
GAATATAGCA CAGAAAAGCA TAAGAAGCAC AAAAAAGAAA AGAAGAAAGT AAAAGACAAA 
GATAGAGACC GGGACCGGGA CAAAGACCGG GACAAGAAAA AATCTCATAG CATCAAGCCA 
GAGAGTTGGT CTAAATCACC CATCTCTTCA GACCAGTCCT TGTCTATGAC AAGCAACACA 
ATTTTATCTA CAGACAGGCC CTCAAGGCTC AGCCCTGACT TTATGATTGG GGAGGAAGAT 
GATGATCTTA TGGATGTGGC CCTGATTGGC AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED1 ( XM_022423023.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022278731.1 Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe38367
Clone ID Related Accession (Same CDS sequence)	XM_014116603.2 , XM_022423023.1 , XM_014116603.1
Accession Version	XM_022423023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mediator of RNA polymerase II transcription subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTGG CTCTCCAATC CTTAGAACAG GATCTATCTA AAATGGCAGT TATGTATTGG 
AAAGCAACCA ATGCTGGTCC CTTGGATAAG ATTCTTCATG GAAGTGTTGG CTATCTTACT 
CCAAGAAGTG GGGGTCATTT AATGAACTTG AAGTACTATG CCTCTCCTTC TGACCTGCTG 
GATGATAAGA CTACATCTCC CATCATTTTG CATGAGAATA ATGTTCCTCG ATCATTGGGC 
ATGAACGCAT CAGTGACAAT TGAAGGAACA TCTGCTATGT ATAAACTCCC AATTGCACCA 
TTAATTATGG GGTCACATCC AGTTGACAAC AAATGGACCC CCTCCTTCTC CTCAATCACC 
AGTGCCAACA GTGTTGATCT TCCTGCCTGT TTCTTCTTGA AATTTCCCCA ACCAATCCCA 
GTATCTAGAG CATTTGTTCA GAAACTTCAG AACTGCACAG GAATTCCATT GTTTGAAACT 
CAACCAACTT ATGTACCCCT CTATGAACTG ATCACTCAGT TTGAGCTGTC AAAGGACCCT 
GACCCCATAC CTTTGAATCA CAACATGAGA TTTTATGCTG CTCTTCCAGG TCAGCAGCAC 
TGCTATTTCC TCAACAAGGA TGCTCCTCTT CCAGATGGCC GAAGTCTACA GGGAACCCTA 
GTTAGCAAAA TCACCTTTCA GCACCCTGGC CGAGTTCCTC TTATCCTAAA TCTGATCAGA 
CATCAAGTGG CCTATAACAC CCTAATAGGA AGTTGTGTCA AAAGAACTAT TCTGAAAGAA 
GATTCTCCTG GGCTCCTCCA ATTTGAAGTG TGTCCTCTCT CAGAGTCCCG TTTTAGCGTA 
TCTTTTCAGC ACCCTGTGAA TGACTCCCTG GTGTGTGTGG TAATGGATGT GCAGGACTCA 
ACACATGTGA GCTGTAAACT GTACAAGGGG CTGTCAGATG CACTCATCTG CACAGATGAC 
TTCATTGCCA AAGTTGTTCA AAGATGTATG TCCATCCCTG TGACGATGAG GGCTATTCGG 
AGGAAAGCTG AAACCATTCA GGCCGACACC CCAGCACTGT CCCTCATTGC AGAGACAGTT 
GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA 
GGCAACAACC CAATGAGTGG TACCACTACA CCAACCAACA CCTTTCCGGG GGGTCCCATT 
ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG 
GAGGACTTCA GCAAGGTGTC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG 
AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC 
TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAC 
CCTGCCCAGG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCTTCT 
TCCGGCTCAC CCCGGATGGA AATATGCCCG GGAAGCAACA AGACAAAGAA GAAGAAGTCA 
TCAAGATTAC CACCTGACAA ACCCAAGCAC CAGACTGAAG ATGACTTTCA GAGGGAGCTA 
TTTTCAATGG ATGTTGACTC ACAGAACCCT ATCTTTGATG TCAACATGAC AGCAGACACG 
CTGGATACGC CACACATCAC TCCAGCTCCA AGCCAGTGTA GTACTCCCCC AACAACTTAC 
CCGCAACCAG TACCTCACCC CCAACCCAGT ATTCAAAGGA TGGTCCGACT ATCCAGTTCA 
GACAGCATTG GCCCAGATGT AACTGATATC CTTTCAGACA TTGCAGAAGA AGCCTCTAAG 
CTTCCCAGCA CTAATGACGA TTGTCCACCC ATTGGCACCC CTGTTCGAGA TTCTTCAAGC 
TCTGGGCATT CTCAGAGTGC TCTCTTTGAC CCTGATGTCT TTCAAACCAA TAATAATGAA 
AATCCATACA CAGATCCAGC TGACCTAATT GCAGATGCTG CTGGTAGCCC TAGTAGTGAC 
TCTCCTACAA ATCATTTTTT TCCTGATGGA GTAGATTTTA ATCCTGATTT GTTGAACAGC 
CAGAGCCAAA GTGGTTTTGG AGAAGAATAT TTTGATGAAA GCAGCCAAAG CGGAGATAAT 
GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGGTGCC AATGCTTGGA 
GGTGATAATG GGGAGACTAA GTTTAAAGGC AATAGCCAAG CTGACACAGT TGATTTCAGT 
ATTATAGCAG TGGCTGGTAA GGCTTTAGGT CCTGCAGATC TTATGGAGCA TCACAGTGGT 
AGCCAGAGTC CTTTATTGAC TACTGGGGAC TTAGGGAAAG AAAAGACTCA AAAACGAGTA 
AAGGAAGGCA ATGGCACCAG TAACAGTAGT CTGTCAGGGC CAGGATTAGA TAGCAAACCA 
GGGAAGCGTA GTCGGACCCC TTCTAATGAT GGCAAAAGCA AAGATAAGCC TCCAAAGCGG 
AAGAAGGCAG ACACTGAAGG AAAGTCTCCA TCTCACAGTT CTTCTAACCG ACCTTTCACT 
CCACCTACCA GTACGGGTGG ATCCAAATCA CCAGGCAGTT CAGGAAGATC ACAGACTCCC 
CCAGGTGTTG CCACACCACC TATTCCCAAA ATCACTATTC AGATTCCTAA AGGTACCGTG 
ATGGTGGGCA AGCCCTCATC CCACAGTCAG TATACCAGCA GTGGTTCTGT GTCTTCCTCA 
GGCAGTAAAA GCCACCATAG CCATTCTTCC TCCTCTTCTT CCTCCTCTTC TGCTTCCAAC 
TCAGGCAAGA TGAAAAGCAG TAAATCAGAA GGTTCATCAA GTTCCAAGTT AAGTAGCAGT 
GTATATTCTA GCCAAGGGTC TTCTGGATCT AGTCAGTCCA AAAATTCATC CCAGTCTGGG 
GGGAAGCCAG GCTCCTCTCC CATTACCAAG CATGGACTGA GCAGTGGCTC CGGCAGCACC 
AAGATGAAAC CTCAAGGGAA GCCATCATCA CTTATGAATC CTTCTTTAAG TAAACCAAAC 
ATATCCCCTT CTCATTCAAG GCCACCTGGA GGCTCTGATA AGCTTGCCTC TCCAATGAAG 
CCTGTTCCTG GGACTCCCCC ATCTTCTAAA GCCAAGTCCC CCATCAGTTC AGGTTCTGGT 
GGTTCTCATA TGTCTGGAAG TAGTTCAAGC ACTGGCATGA AGTCATCTTC AGGGTTAGGA 
TCTTCAGGCT CATTGTCTCA GAAAACTCCC CCATCATCTA ATTCTTGTAC AGCATCTTCC 
TCTTCCTTTT CCTCAAGTGG CTCTTCCATG TCATCCTCTC AGAACCAGCA CGGGAGTTCC 
AAAGGGAAAT CCCCCAGCAG AAATAAGAAG CCGTCCTTAA CAGCCGTCAT AGATAAACTG 
AAGCATGGGG TTGTCACCAG TGGCCCTGGA GGTGAAGACC CGATGGATGG CCAGATGGGA 
GTGAGCACAA ATTCTTCTAG CCATCCTATG TCCTCCAAAC ATAACATGTC AGGAGGAGAG 
TTCCAGGGTA AGCGTGAGAA AAGTGATAAA GAAAAATCCA AGGTTTCCAC CTCGGGGGGT 
TCAGTGGATT CGTCTAAGAA GACCTCAGAG TCAAAAAATG TGGGGAGCAC AGGTGTGGCA 
AAAATTATCA TCAGCAAGCA TGATGGGGGT TCCCCCAGCA TTAAAGCCAA AGTGACTTTG 
CAGAAACCTG GGGAAAGTAG TGGAGAAGGG CTCAGACCTC AGATGGCCTC TTCCAAAAAC 
TATGGCTCTC CACTCATCAG TGGTTCCACT CCAAAGCATG AGCGTGGCTC TCCTAGCCAT 
AGTAAGTCAC CAGCATATAC CCCCCAGAAT CTGGACAGTG AAAGTGAGTC AGGCTCCTCC 
ATAGCAGAGA AATCTTATCA GAACAGTCCG AGCTCAGATG ATGGTATCCG ACCTCTTCCA 
GAATATAGCA CAGAAAAGCA TAAGAAGCAC AAAAAAGAAA AGAAGAAAGT AAAAGACAAA 
GATAGAGACC GGGACCGGGA CAAAGACCGG GACAAGAAAA AATCTCATAG CATCAAGCCA 
GAGAGTTGGT CTAAATCACC CATCTCTTCA GACCAGTCCT TGTCTATGAC AAGCAACACA 
ATTTTATCTA CAGACAGGCC CTCAAGGCTC AGCCCTGACT TTATGATTGG GGAGGAAGAT 
GATGATCTTA TGGATGTGGC CCTGATTGGC AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022278731.1
CDS	634..4869
Translation	MYLALQSLEQDLSKMAVMYWKATNAGPLDKILHGSVGYLTPRSGGHLMNLKYYASPSDLLDDKTTSPIILHENNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYVPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEICPGSNKTKKKKSSRLPPDKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTNDDCPPIGTPVRDSSSSGHSQSALFDPDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNSQADTVDFSIIAVAGKALGPADLMEHHSGSQSPLLTTGDLGKEKTQKRVKEGNGTSNSSLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSSSASNSGKMKSSKSEGSSSSKLSSSVYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSGSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGSSSSTGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPMDGQMGVSTNSSSHPMSSKHNMSGGEFQGKREKSDKEKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSTDRPSRLSPDFMIGEEDDDLMDVALIGN

Target ORF information:

RefSeq Version	XM_022423023.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022423023.1

ORF Insert Sequence:

ATGTACTTGG CTCTCCAATC CTTAGAACAG GATCTATCTA AAATGGCAGT TATGTATTGG 
AAAGCAACCA ATGCTGGTCC CTTGGATAAG ATTCTTCATG GAAGTGTTGG CTATCTTACT 
CCAAGAAGTG GGGGTCATTT AATGAACTTG AAGTACTATG CCTCTCCTTC TGACCTGCTG 
GATGATAAGA CTACATCTCC CATCATTTTG CATGAGAATA ATGTTCCTCG ATCATTGGGC 
ATGAACGCAT CAGTGACAAT TGAAGGAACA TCTGCTATGT ATAAACTCCC AATTGCACCA 
TTAATTATGG GGTCACATCC AGTTGACAAC AAATGGACCC CCTCCTTCTC CTCAATCACC 
AGTGCCAACA GTGTTGATCT TCCTGCCTGT TTCTTCTTGA AATTTCCCCA ACCAATCCCA 
GTATCTAGAG CATTTGTTCA GAAACTTCAG AACTGCACAG GAATTCCATT GTTTGAAACT 
CAACCAACTT ATGTACCCCT CTATGAACTG ATCACTCAGT TTGAGCTGTC AAAGGACCCT 
GACCCCATAC CTTTGAATCA CAACATGAGA TTTTATGCTG CTCTTCCAGG TCAGCAGCAC 
TGCTATTTCC TCAACAAGGA TGCTCCTCTT CCAGATGGCC GAAGTCTACA GGGAACCCTA 
GTTAGCAAAA TCACCTTTCA GCACCCTGGC CGAGTTCCTC TTATCCTAAA TCTGATCAGA 
CATCAAGTGG CCTATAACAC CCTAATAGGA AGTTGTGTCA AAAGAACTAT TCTGAAAGAA 
GATTCTCCTG GGCTCCTCCA ATTTGAAGTG TGTCCTCTCT CAGAGTCCCG TTTTAGCGTA 
TCTTTTCAGC ACCCTGTGAA TGACTCCCTG GTGTGTGTGG TAATGGATGT GCAGGACTCA 
ACACATGTGA GCTGTAAACT GTACAAGGGG CTGTCAGATG CACTCATCTG CACAGATGAC 
TTCATTGCCA AAGTTGTTCA AAGATGTATG TCCATCCCTG TGACGATGAG GGCTATTCGG 
AGGAAAGCTG AAACCATTCA GGCCGACACC CCAGCACTGT CCCTCATTGC AGAGACAGTT 
GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA 
GGCAACAACC CAATGAGTGG TACCACTACA CCAACCAACA CCTTTCCGGG GGGTCCCATT 
ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG 
GAGGACTTCA GCAAGGTGTC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG 
AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC 
TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAC 
CCTGCCCAGG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCTTCT 
TCCGGCTCAC CCCGGATGGA AATATGCCCG GGAAGCAACA AGACAAAGAA GAAGAAGTCA 
TCAAGATTAC CACCTGACAA ACCCAAGCAC CAGACTGAAG ATGACTTTCA GAGGGAGCTA 
TTTTCAATGG ATGTTGACTC ACAGAACCCT ATCTTTGATG TCAACATGAC AGCAGACACG 
CTGGATACGC CACACATCAC TCCAGCTCCA AGCCAGTGTA GTACTCCCCC AACAACTTAC 
CCGCAACCAG TACCTCACCC CCAACCCAGT ATTCAAAGGA TGGTCCGACT ATCCAGTTCA 
GACAGCATTG GCCCAGATGT AACTGATATC CTTTCAGACA TTGCAGAAGA AGCCTCTAAG 
CTTCCCAGCA CTAATGACGA TTGTCCACCC ATTGGCACCC CTGTTCGAGA TTCTTCAAGC 
TCTGGGCATT CTCAGAGTGC TCTCTTTGAC CCTGATGTCT TTCAAACCAA TAATAATGAA 
AATCCATACA CAGATCCAGC TGACCTAATT GCAGATGCTG CTGGTAGCCC TAGTAGTGAC 
TCTCCTACAA ATCATTTTTT TCCTGATGGA GTAGATTTTA ATCCTGATTT GTTGAACAGC 
CAGAGCCAAA GTGGTTTTGG AGAAGAATAT TTTGATGAAA GCAGCCAAAG CGGAGATAAT 
GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGGTGCC AATGCTTGGA 
GGTGATAATG GGGAGACTAA GTTTAAAGGC AATAGCCAAG CTGACACAGT TGATTTCAGT 
ATTATAGCAG TGGCTGGTAA GGCTTTAGGT CCTGCAGATC TTATGGAGCA TCACAGTGGT 
AGCCAGAGTC CTTTATTGAC TACTGGGGAC TTAGGGAAAG AAAAGACTCA AAAACGAGTA 
AAGGAAGGCA ATGGCACCAG TAACAGTAGT CTGTCAGGGC CAGGATTAGA TAGCAAACCA 
GGGAAGCGTA GTCGGACCCC TTCTAATGAT GGCAAAAGCA AAGATAAGCC TCCAAAGCGG 
AAGAAGGCAG ACACTGAAGG AAAGTCTCCA TCTCACAGTT CTTCTAACCG ACCTTTCACT 
CCACCTACCA GTACGGGTGG ATCCAAATCA CCAGGCAGTT CAGGAAGATC ACAGACTCCC 
CCAGGTGTTG CCACACCACC TATTCCCAAA ATCACTATTC AGATTCCTAA AGGTACCGTG 
ATGGTGGGCA AGCCCTCATC CCACAGTCAG TATACCAGCA GTGGTTCTGT GTCTTCCTCA 
GGCAGTAAAA GCCACCATAG CCATTCTTCC TCCTCTTCTT CCTCCTCTTC TGCTTCCAAC 
TCAGGCAAGA TGAAAAGCAG TAAATCAGAA GGTTCATCAA GTTCCAAGTT AAGTAGCAGT 
GTATATTCTA GCCAAGGGTC TTCTGGATCT AGTCAGTCCA AAAATTCATC CCAGTCTGGG 
GGGAAGCCAG GCTCCTCTCC CATTACCAAG CATGGACTGA GCAGTGGCTC CGGCAGCACC 
AAGATGAAAC CTCAAGGGAA GCCATCATCA CTTATGAATC CTTCTTTAAG TAAACCAAAC 
ATATCCCCTT CTCATTCAAG GCCACCTGGA GGCTCTGATA AGCTTGCCTC TCCAATGAAG 
CCTGTTCCTG GGACTCCCCC ATCTTCTAAA GCCAAGTCCC CCATCAGTTC AGGTTCTGGT 
GGTTCTCATA TGTCTGGAAG TAGTTCAAGC ACTGGCATGA AGTCATCTTC AGGGTTAGGA 
TCTTCAGGCT CATTGTCTCA GAAAACTCCC CCATCATCTA ATTCTTGTAC AGCATCTTCC 
TCTTCCTTTT CCTCAAGTGG CTCTTCCATG TCATCCTCTC AGAACCAGCA CGGGAGTTCC 
AAAGGGAAAT CCCCCAGCAG AAATAAGAAG CCGTCCTTAA CAGCCGTCAT AGATAAACTG 
AAGCATGGGG TTGTCACCAG TGGCCCTGGA GGTGAAGACC CGATGGATGG CCAGATGGGA 
GTGAGCACAA ATTCTTCTAG CCATCCTATG TCCTCCAAAC ATAACATGTC AGGAGGAGAG 
TTCCAGGGTA AGCGTGAGAA AAGTGATAAA GAAAAATCCA AGGTTTCCAC CTCGGGGGGT 
TCAGTGGATT CGTCTAAGAA GACCTCAGAG TCAAAAAATG TGGGGAGCAC AGGTGTGGCA 
AAAATTATCA TCAGCAAGCA TGATGGGGGT TCCCCCAGCA TTAAAGCCAA AGTGACTTTG 
CAGAAACCTG GGGAAAGTAG TGGAGAAGGG CTCAGACCTC AGATGGCCTC TTCCAAAAAC 
TATGGCTCTC CACTCATCAG TGGTTCCACT CCAAAGCATG AGCGTGGCTC TCCTAGCCAT 
AGTAAGTCAC CAGCATATAC CCCCCAGAAT CTGGACAGTG AAAGTGAGTC AGGCTCCTCC 
ATAGCAGAGA AATCTTATCA GAACAGTCCG AGCTCAGATG ATGGTATCCG ACCTCTTCCA 
GAATATAGCA CAGAAAAGCA TAAGAAGCAC AAAAAAGAAA AGAAGAAAGT AAAAGACAAA 
GATAGAGACC GGGACCGGGA CAAAGACCGG GACAAGAAAA AATCTCATAG CATCAAGCCA 
GAGAGTTGGT CTAAATCACC CATCTCTTCA GACCAGTCCT TGTCTATGAC AAGCAACACA 
ATTTTATCTA CAGACAGGCC CTCAAGGCTC AGCCCTGACT TTATGATTGG GGAGGAAGAT 
GATGATCTTA TGGATGTGGC CCTGATTGGC AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED1 ( XM_014116603.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013972078.1 Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X2, mRNA.

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CloneID	OCe38367
Clone ID Related Accession (Same CDS sequence)	XM_014116603.2 , XM_022423023.1 , XM_014116603.1
Accession Version	XM_014116603.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	mediator of RNA polymerase II transcription subunit 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTGG CTCTCCAATC CTTAGAACAG GATCTATCTA AAATGGCAGT TATGTATTGG 
AAAGCAACCA ATGCTGGTCC CTTGGATAAG ATTCTTCATG GAAGTGTTGG CTATCTTACT 
CCAAGAAGTG GGGGTCATTT AATGAACTTG AAGTACTATG CCTCTCCTTC TGACCTGCTG 
GATGATAAGA CTACATCTCC CATCATTTTG CATGAGAATA ATGTTCCTCG ATCATTGGGC 
ATGAACGCAT CAGTGACAAT TGAAGGAACA TCTGCTATGT ATAAACTCCC AATTGCACCA 
TTAATTATGG GGTCACATCC AGTTGACAAC AAATGGACCC CCTCCTTCTC CTCAATCACC 
AGTGCCAACA GTGTTGATCT TCCTGCCTGT TTCTTCTTGA AATTTCCCCA ACCAATCCCA 
GTATCTAGAG CATTTGTTCA GAAACTTCAG AACTGCACAG GAATTCCATT GTTTGAAACT 
CAACCAACTT ATGTACCCCT CTATGAACTG ATCACTCAGT TTGAGCTGTC AAAGGACCCT 
GACCCCATAC CTTTGAATCA CAACATGAGA TTTTATGCTG CTCTTCCAGG TCAGCAGCAC 
TGCTATTTCC TCAACAAGGA TGCTCCTCTT CCAGATGGCC GAAGTCTACA GGGAACCCTA 
GTTAGCAAAA TCACCTTTCA GCACCCTGGC CGAGTTCCTC TTATCCTAAA TCTGATCAGA 
CATCAAGTGG CCTATAACAC CCTAATAGGA AGTTGTGTCA AAAGAACTAT TCTGAAAGAA 
GATTCTCCTG GGCTCCTCCA ATTTGAAGTG TGTCCTCTCT CAGAGTCCCG TTTTAGCGTA 
TCTTTTCAGC ACCCTGTGAA TGACTCCCTG GTGTGTGTGG TAATGGATGT GCAGGACTCA 
ACACATGTGA GCTGTAAACT GTACAAGGGG CTGTCAGATG CACTCATCTG CACAGATGAC 
TTCATTGCCA AAGTTGTTCA AAGATGTATG TCCATCCCTG TGACGATGAG GGCTATTCGG 
AGGAAAGCTG AAACCATTCA GGCCGACACC CCAGCACTGT CCCTCATTGC AGAGACAGTT 
GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA 
GGCAACAACC CAATGAGTGG TACCACTACA CCAACCAACA CCTTTCCGGG GGGTCCCATT 
ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG 
GAGGACTTCA GCAAGGTGTC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG 
AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC 
TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAC 
CCTGCCCAGG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCTTCT 
TCCGGCTCAC CCCGGATGGA AATATGCCCG GGAAGCAACA AGACAAAGAA GAAGAAGTCA 
TCAAGATTAC CACCTGACAA ACCCAAGCAC CAGACTGAAG ATGACTTTCA GAGGGAGCTA 
TTTTCAATGG ATGTTGACTC ACAGAACCCT ATCTTTGATG TCAACATGAC AGCAGACACG 
CTGGATACGC CACACATCAC TCCAGCTCCA AGCCAGTGTA GTACTCCCCC AACAACTTAC 
CCGCAACCAG TACCTCACCC CCAACCCAGT ATTCAAAGGA TGGTCCGACT ATCCAGTTCA 
GACAGCATTG GCCCAGATGT AACTGATATC CTTTCAGACA TTGCAGAAGA AGCCTCTAAG 
CTTCCCAGCA CTAATGACGA TTGTCCACCC ATTGGCACCC CTGTTCGAGA TTCTTCAAGC 
TCTGGGCATT CTCAGAGTGC TCTCTTTGAC CCTGATGTCT TTCAAACCAA TAATAATGAA 
AATCCATACA CAGATCCAGC TGACCTAATT GCAGATGCTG CTGGTAGCCC TAGTAGTGAC 
TCTCCTACAA ATCATTTTTT TCCTGATGGA GTAGATTTTA ATCCTGATTT GTTGAACAGC 
CAGAGCCAAA GTGGTTTTGG AGAAGAATAT TTTGATGAAA GCAGCCAAAG CGGAGATAAT 
GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGGTGCC AATGCTTGGA 
GGTGATAATG GGGAGACTAA GTTTAAAGGC AATAGCCAAG CTGACACAGT TGATTTCAGT 
ATTATAGCAG TGGCTGGTAA GGCTTTAGGT CCTGCAGATC TTATGGAGCA TCACAGTGGT 
AGCCAGAGTC CTTTATTGAC TACTGGGGAC TTAGGGAAAG AAAAGACTCA AAAACGAGTA 
AAGGAAGGCA ATGGCACCAG TAACAGTAGT CTGTCAGGGC CAGGATTAGA TAGCAAACCA 
GGGAAGCGTA GTCGGACCCC TTCTAATGAT GGCAAAAGCA AAGATAAGCC TCCAAAGCGG 
AAGAAGGCAG ACACTGAAGG AAAGTCTCCA TCTCACAGTT CTTCTAACCG ACCTTTCACT 
CCACCTACCA GTACGGGTGG ATCCAAATCA CCAGGCAGTT CAGGAAGATC ACAGACTCCC 
CCAGGTGTTG CCACACCACC TATTCCCAAA ATCACTATTC AGATTCCTAA AGGTACCGTG 
ATGGTGGGCA AGCCCTCATC CCACAGTCAG TATACCAGCA GTGGTTCTGT GTCTTCCTCA 
GGCAGTAAAA GCCACCATAG CCATTCTTCC TCCTCTTCTT CCTCCTCTTC TGCTTCCAAC 
TCAGGCAAGA TGAAAAGCAG TAAATCAGAA GGTTCATCAA GTTCCAAGTT AAGTAGCAGT 
GTATATTCTA GCCAAGGGTC TTCTGGATCT AGTCAGTCCA AAAATTCATC CCAGTCTGGG 
GGGAAGCCAG GCTCCTCTCC CATTACCAAG CATGGACTGA GCAGTGGCTC CGGCAGCACC 
AAGATGAAAC CTCAAGGGAA GCCATCATCA CTTATGAATC CTTCTTTAAG TAAACCAAAC 
ATATCCCCTT CTCATTCAAG GCCACCTGGA GGCTCTGATA AGCTTGCCTC TCCAATGAAG 
CCTGTTCCTG GGACTCCCCC ATCTTCTAAA GCCAAGTCCC CCATCAGTTC AGGTTCTGGT 
GGTTCTCATA TGTCTGGAAG TAGTTCAAGC ACTGGCATGA AGTCATCTTC AGGGTTAGGA 
TCTTCAGGCT CATTGTCTCA GAAAACTCCC CCATCATCTA ATTCTTGTAC AGCATCTTCC 
TCTTCCTTTT CCTCAAGTGG CTCTTCCATG TCATCCTCTC AGAACCAGCA CGGGAGTTCC 
AAAGGGAAAT CCCCCAGCAG AAATAAGAAG CCGTCCTTAA CAGCCGTCAT AGATAAACTG 
AAGCATGGGG TTGTCACCAG TGGCCCTGGA GGTGAAGACC CGATGGATGG CCAGATGGGA 
GTGAGCACAA ATTCTTCTAG CCATCCTATG TCCTCCAAAC ATAACATGTC AGGAGGAGAG 
TTCCAGGGTA AGCGTGAGAA AAGTGATAAA GAAAAATCCA AGGTTTCCAC CTCGGGGGGT 
TCAGTGGATT CGTCTAAGAA GACCTCAGAG TCAAAAAATG TGGGGAGCAC AGGTGTGGCA 
AAAATTATCA TCAGCAAGCA TGATGGGGGT TCCCCCAGCA TTAAAGCCAA AGTGACTTTG 
CAGAAACCTG GGGAAAGTAG TGGAGAAGGG CTCAGACCTC AGATGGCCTC TTCCAAAAAC 
TATGGCTCTC CACTCATCAG TGGTTCCACT CCAAAGCATG AGCGTGGCTC TCCTAGCCAT 
AGTAAGTCAC CAGCATATAC CCCCCAGAAT CTGGACAGTG AAAGTGAGTC AGGCTCCTCC 
ATAGCAGAGA AATCTTATCA GAACAGTCCG AGCTCAGATG ATGGTATCCG ACCTCTTCCA 
GAATATAGCA CAGAAAAGCA TAAGAAGCAC AAAAAAGAAA AGAAGAAAGT AAAAGACAAA 
GATAGAGACC GGGACCGGGA CAAAGACCGG GACAAGAAAA AATCTCATAG CATCAAGCCA 
GAGAGTTGGT CTAAATCACC CATCTCTTCA GACCAGTCCT TGTCTATGAC AAGCAACACA 
ATTTTATCTA CAGACAGGCC CTCAAGGCTC AGCCCTGACT TTATGATTGG GGAGGAAGAT 
GATGATCTTA TGGATGTGGC CCTGATTGGC AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972078.1
CDS	188..4423
Translation	MYLALQSLEQDLSKMAVMYWKATNAGPLDKILHGSVGYLTPRSGGHLMNLKYYASPSDLLDDKTTSPIILHENNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYVPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEICPGSNKTKKKKSSRLPPDKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTNDDCPPIGTPVRDSSSSGHSQSALFDPDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNSQADTVDFSIIAVAGKALGPADLMEHHSGSQSPLLTTGDLGKEKTQKRVKEGNGTSNSSLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSSSASNSGKMKSSKSEGSSSSKLSSSVYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSGSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGSSSSTGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPMDGQMGVSTNSSSHPMSSKHNMSGGEFQGKREKSDKEKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSTDRPSRLSPDFMIGEEDDDLMDVALIGN

Target ORF information:

RefSeq Version	XM_014116603.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116603.1

ORF Insert Sequence:

ATGTACTTGG CTCTCCAATC CTTAGAACAG GATCTATCTA AAATGGCAGT TATGTATTGG 
AAAGCAACCA ATGCTGGTCC CTTGGATAAG ATTCTTCATG GAAGTGTTGG CTATCTTACT 
CCAAGAAGTG GGGGTCATTT AATGAACTTG AAGTACTATG CCTCTCCTTC TGACCTGCTG 
GATGATAAGA CTACATCTCC CATCATTTTG CATGAGAATA ATGTTCCTCG ATCATTGGGC 
ATGAACGCAT CAGTGACAAT TGAAGGAACA TCTGCTATGT ATAAACTCCC AATTGCACCA 
TTAATTATGG GGTCACATCC AGTTGACAAC AAATGGACCC CCTCCTTCTC CTCAATCACC 
AGTGCCAACA GTGTTGATCT TCCTGCCTGT TTCTTCTTGA AATTTCCCCA ACCAATCCCA 
GTATCTAGAG CATTTGTTCA GAAACTTCAG AACTGCACAG GAATTCCATT GTTTGAAACT 
CAACCAACTT ATGTACCCCT CTATGAACTG ATCACTCAGT TTGAGCTGTC AAAGGACCCT 
GACCCCATAC CTTTGAATCA CAACATGAGA TTTTATGCTG CTCTTCCAGG TCAGCAGCAC 
TGCTATTTCC TCAACAAGGA TGCTCCTCTT CCAGATGGCC GAAGTCTACA GGGAACCCTA 
GTTAGCAAAA TCACCTTTCA GCACCCTGGC CGAGTTCCTC TTATCCTAAA TCTGATCAGA 
CATCAAGTGG CCTATAACAC CCTAATAGGA AGTTGTGTCA AAAGAACTAT TCTGAAAGAA 
GATTCTCCTG GGCTCCTCCA ATTTGAAGTG TGTCCTCTCT CAGAGTCCCG TTTTAGCGTA 
TCTTTTCAGC ACCCTGTGAA TGACTCCCTG GTGTGTGTGG TAATGGATGT GCAGGACTCA 
ACACATGTGA GCTGTAAACT GTACAAGGGG CTGTCAGATG CACTCATCTG CACAGATGAC 
TTCATTGCCA AAGTTGTTCA AAGATGTATG TCCATCCCTG TGACGATGAG GGCTATTCGG 
AGGAAAGCTG AAACCATTCA GGCCGACACC CCAGCACTGT CCCTCATTGC AGAGACAGTT 
GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA 
GGCAACAACC CAATGAGTGG TACCACTACA CCAACCAACA CCTTTCCGGG GGGTCCCATT 
ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG 
GAGGACTTCA GCAAGGTGTC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG 
AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC 
TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAC 
CCTGCCCAGG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCTTCT 
TCCGGCTCAC CCCGGATGGA AATATGCCCG GGAAGCAACA AGACAAAGAA GAAGAAGTCA 
TCAAGATTAC CACCTGACAA ACCCAAGCAC CAGACTGAAG ATGACTTTCA GAGGGAGCTA 
TTTTCAATGG ATGTTGACTC ACAGAACCCT ATCTTTGATG TCAACATGAC AGCAGACACG 
CTGGATACGC CACACATCAC TCCAGCTCCA AGCCAGTGTA GTACTCCCCC AACAACTTAC 
CCGCAACCAG TACCTCACCC CCAACCCAGT ATTCAAAGGA TGGTCCGACT ATCCAGTTCA 
GACAGCATTG GCCCAGATGT AACTGATATC CTTTCAGACA TTGCAGAAGA AGCCTCTAAG 
CTTCCCAGCA CTAATGACGA TTGTCCACCC ATTGGCACCC CTGTTCGAGA TTCTTCAAGC 
TCTGGGCATT CTCAGAGTGC TCTCTTTGAC CCTGATGTCT TTCAAACCAA TAATAATGAA 
AATCCATACA CAGATCCAGC TGACCTAATT GCAGATGCTG CTGGTAGCCC TAGTAGTGAC 
TCTCCTACAA ATCATTTTTT TCCTGATGGA GTAGATTTTA ATCCTGATTT GTTGAACAGC 
CAGAGCCAAA GTGGTTTTGG AGAAGAATAT TTTGATGAAA GCAGCCAAAG CGGAGATAAT 
GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGGTGCC AATGCTTGGA 
GGTGATAATG GGGAGACTAA GTTTAAAGGC AATAGCCAAG CTGACACAGT TGATTTCAGT 
ATTATAGCAG TGGCTGGTAA GGCTTTAGGT CCTGCAGATC TTATGGAGCA TCACAGTGGT 
AGCCAGAGTC CTTTATTGAC TACTGGGGAC TTAGGGAAAG AAAAGACTCA AAAACGAGTA 
AAGGAAGGCA ATGGCACCAG TAACAGTAGT CTGTCAGGGC CAGGATTAGA TAGCAAACCA 
GGGAAGCGTA GTCGGACCCC TTCTAATGAT GGCAAAAGCA AAGATAAGCC TCCAAAGCGG 
AAGAAGGCAG ACACTGAAGG AAAGTCTCCA TCTCACAGTT CTTCTAACCG ACCTTTCACT 
CCACCTACCA GTACGGGTGG ATCCAAATCA CCAGGCAGTT CAGGAAGATC ACAGACTCCC 
CCAGGTGTTG CCACACCACC TATTCCCAAA ATCACTATTC AGATTCCTAA AGGTACCGTG 
ATGGTGGGCA AGCCCTCATC CCACAGTCAG TATACCAGCA GTGGTTCTGT GTCTTCCTCA 
GGCAGTAAAA GCCACCATAG CCATTCTTCC TCCTCTTCTT CCTCCTCTTC TGCTTCCAAC 
TCAGGCAAGA TGAAAAGCAG TAAATCAGAA GGTTCATCAA GTTCCAAGTT AAGTAGCAGT 
GTATATTCTA GCCAAGGGTC TTCTGGATCT AGTCAGTCCA AAAATTCATC CCAGTCTGGG 
GGGAAGCCAG GCTCCTCTCC CATTACCAAG CATGGACTGA GCAGTGGCTC CGGCAGCACC 
AAGATGAAAC CTCAAGGGAA GCCATCATCA CTTATGAATC CTTCTTTAAG TAAACCAAAC 
ATATCCCCTT CTCATTCAAG GCCACCTGGA GGCTCTGATA AGCTTGCCTC TCCAATGAAG 
CCTGTTCCTG GGACTCCCCC ATCTTCTAAA GCCAAGTCCC CCATCAGTTC AGGTTCTGGT 
GGTTCTCATA TGTCTGGAAG TAGTTCAAGC ACTGGCATGA AGTCATCTTC AGGGTTAGGA 
TCTTCAGGCT CATTGTCTCA GAAAACTCCC CCATCATCTA ATTCTTGTAC AGCATCTTCC 
TCTTCCTTTT CCTCAAGTGG CTCTTCCATG TCATCCTCTC AGAACCAGCA CGGGAGTTCC 
AAAGGGAAAT CCCCCAGCAG AAATAAGAAG CCGTCCTTAA CAGCCGTCAT AGATAAACTG 
AAGCATGGGG TTGTCACCAG TGGCCCTGGA GGTGAAGACC CGATGGATGG CCAGATGGGA 
GTGAGCACAA ATTCTTCTAG CCATCCTATG TCCTCCAAAC ATAACATGTC AGGAGGAGAG 
TTCCAGGGTA AGCGTGAGAA AAGTGATAAA GAAAAATCCA AGGTTTCCAC CTCGGGGGGT 
TCAGTGGATT CGTCTAAGAA GACCTCAGAG TCAAAAAATG TGGGGAGCAC AGGTGTGGCA 
AAAATTATCA TCAGCAAGCA TGATGGGGGT TCCCCCAGCA TTAAAGCCAA AGTGACTTTG 
CAGAAACCTG GGGAAAGTAG TGGAGAAGGG CTCAGACCTC AGATGGCCTC TTCCAAAAAC 
TATGGCTCTC CACTCATCAG TGGTTCCACT CCAAAGCATG AGCGTGGCTC TCCTAGCCAT 
AGTAAGTCAC CAGCATATAC CCCCCAGAAT CTGGACAGTG AAAGTGAGTC AGGCTCCTCC 
ATAGCAGAGA AATCTTATCA GAACAGTCCG AGCTCAGATG ATGGTATCCG ACCTCTTCCA 
GAATATAGCA CAGAAAAGCA TAAGAAGCAC AAAAAAGAAA AGAAGAAAGT AAAAGACAAA 
GATAGAGACC GGGACCGGGA CAAAGACCGG GACAAGAAAA AATCTCATAG CATCAAGCCA 
GAGAGTTGGT CTAAATCACC CATCTCTTCA GACCAGTCCT TGTCTATGAC AAGCAACACA 
ATTTTATCTA CAGACAGGCC CTCAAGGCTC AGCCCTGACT TTATGATTGG GGAGGAAGAT 
GATGATCTTA TGGATGTGGC CCTGATTGGC AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED1 ( XM_858916.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_864009.2 Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X1, mRNA.

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CloneID	OCe11780
Clone ID Related Accession (Same CDS sequence)	XM_858916.4 , XM_858916.5
Accession Version	XM_858916.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	mediator of RNA polymerase II transcription subunit 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_858916.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCTC AGGGGGAAAC CGAGGAATCG GAGAAGCTGA GTAAGATGAG TTCTCTCTTG 
GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCTTGGA GTGAAACCAT TAAGCTTGTG 
CGTCAAGTCA TGGAGAAGAG AGTTGTGATG AGTTCTGGAG GGCACCAACA TTTGGTCAGC 
TGTTTGGAGA CTTTGCAGAA AGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGACCGC 
TTGGAGTCTA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA 
TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT 
TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCAGA ACTTGTACAG 
CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGTCT TGTTAATCTT 
TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA 
GAACAGGATC TATCTAAAAT GGCAGTTATG TATTGGAAAG CAACCAATGC TGGTCCCTTG 
GATAAGATTC TTCATGGAAG TGTTGGCTAT CTTACTCCAA GAAGTGGGGG TCATTTAATG 
AACTTGAAGT ACTATGCCTC TCCTTCTGAC CTGCTGGATG ATAAGACTAC ATCTCCCATC 
ATTTTGCATG AGAATAATGT TCCTCGATCA TTGGGCATGA ACGCATCAGT GACAATTGAA 
GGAACATCTG CTATGTATAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT 
GACAACAAAT GGACCCCCTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT 
GCCTGTTTCT TCTTGAAATT TCCCCAACCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA 
CTTCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGT ACCCCTCTAT 
GAACTGATCA CTCAGTTTGA GCTGTCAAAG GACCCTGACC CCATACCTTT GAATCACAAC 
ATGAGATTTT ATGCTGCTCT TCCAGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT 
CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTAGTTA GCAAAATCAC CTTTCAGCAC 
CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACATC AAGTGGCCTA TAACACCCTA 
ATAGGAAGTT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT CCTCCAATTT 
GAAGTGTGTC CTCTCTCAGA GTCCCGTTTT AGCGTATCTT TTCAGCACCC TGTGAATGAC 
TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTGTAC 
AAGGGGCTGT CAGATGCACT CATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA 
TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAGGCC 
GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG 
CCCCCGGCTA GCAGCCCAGG GTATGGCATG ACCACAGGCA ACAACCCAAT GAGTGGTACC 
ACTACACCAA CCAACACCTT TCCGGGGGGT CCCATTACCA CCTTGTTTAA TATGAGCATG 
AGCATCAAAG ATCGGCATGA GTCGGTGGGC CATGGGGAGG ACTTCAGCAA GGTGTCTCAG 
AACCCAATTC TTACCAGTTT GTTGCAAATC ACAGGGAACG GGGGGTCTAC CATTGGCTCG 
AGTCCGACCC CTCCTCATCA CACGCCGCCA CCTGTCTCTT CGATGGCCGG CAACACCAAG 
AACCACCCGA TGCTCATGAA CCTTCTTAAA GATAACCCTG CCCAGGATTT CTCAACCCTT 
TATGGAAGCA GCCCTTTAGA AAGGCAGAAC TCTTCTTCCG GCTCACCCCG GATGGAAATA 
TGCCCGGGAA GCAACAAGAC AAAGAAGAAG AAGTCATCAA GATTACCACC TGACAAACCC 
AAGCACCAGA CTGAAGATGA CTTTCAGAGG GAGCTATTTT CAATGGATGT TGACTCACAG 
AACCCTATCT TTGATGTCAA CATGACAGCA GACACGCTGG ATACGCCACA CATCACTCCA 
GCTCCAAGCC AGTGTAGTAC TCCCCCAACA ACTTACCCGC AACCAGTACC TCACCCCCAA 
CCCAGTATTC AAAGGATGGT CCGACTATCC AGTTCAGACA GCATTGGCCC AGATGTAACT 
GATATCCTTT CAGACATTGC AGAAGAAGCC TCTAAGCTTC CCAGCACTAA TGACGATTGT 
CCACCCATTG GCACCCCTGT TCGAGATTCT TCAAGCTCTG GGCATTCTCA GAGTGCTCTC 
TTTGACCCTG ATGTCTTTCA AACCAATAAT AATGAAAATC CATACACAGA TCCAGCTGAC 
CTAATTGCAG ATGCTGCTGG TAGCCCTAGT AGTGACTCTC CTACAAATCA TTTTTTTCCT 
GATGGAGTAG ATTTTAATCC TGATTTGTTG AACAGCCAGA GCCAAAGTGG TTTTGGAGAA 
GAATATTTTG ATGAAAGCAG CCAAAGCGGA GATAATGATG ATTTCAAAGG ATTTGCATCT 
CAGGCACTAA ATACTTTGGG GGTGCCAATG CTTGGAGGTG ATAATGGGGA GACTAAGTTT 
AAAGGCAATA GCCAAGCTGA CACAGTTGAT TTCAGTATTA TAGCAGTGGC TGGTAAGGCT 
TTAGGTCCTG CAGATCTTAT GGAGCATCAC AGTGGTAGCC AGAGTCCTTT ATTGACTACT 
GGGGACTTAG GGAAAGAAAA GACTCAAAAA CGAGTAAAGG AAGGCAATGG CACCAGTAAC 
AGTAGTCTGT CAGGGCCAGG ATTAGATAGC AAACCAGGGA AGCGTAGTCG GACCCCTTCT 
AATGATGGCA AAAGCAAAGA TAAGCCTCCA AAGCGGAAGA AGGCAGACAC TGAAGGAAAG 
TCTCCATCTC ACAGTTCTTC TAACCGACCT TTCACTCCAC CTACCAGTAC GGGTGGATCC 
AAATCACCAG GCAGTTCAGG AAGATCACAG ACTCCCCCAG GTGTTGCCAC ACCACCTATT 
CCCAAAATCA CTATTCAGAT TCCTAAAGGT ACCGTGATGG TGGGCAAGCC CTCATCCCAC 
AGTCAGTATA CCAGCAGTGG TTCTGTGTCT TCCTCAGGCA GTAAAAGCCA CCATAGCCAT 
TCTTCCTCCT CTTCTTCCTC CTCTTCTGCT TCCAACTCAG GCAAGATGAA AAGCAGTAAA 
TCAGAAGGTT CATCAAGTTC CAAGTTAAGT AGCAGTGTAT ATTCTAGCCA AGGGTCTTCT 
GGATCTAGTC AGTCCAAAAA TTCATCCCAG TCTGGGGGGA AGCCAGGCTC CTCTCCCATT 
ACCAAGCATG GACTGAGCAG TGGCTCCGGC AGCACCAAGA TGAAACCTCA AGGGAAGCCA 
TCATCACTTA TGAATCCTTC TTTAAGTAAA CCAAACATAT CCCCTTCTCA TTCAAGGCCA 
CCTGGAGGCT CTGATAAGCT TGCCTCTCCA ATGAAGCCTG TTCCTGGGAC TCCCCCATCT 
TCTAAAGCCA AGTCCCCCAT CAGTTCAGGT TCTGGTGGTT CTCATATGTC TGGAAGTAGT 
TCAAGCACTG GCATGAAGTC ATCTTCAGGG TTAGGATCTT CAGGCTCATT GTCTCAGAAA 
ACTCCCCCAT CATCTAATTC TTGTACAGCA TCTTCCTCTT CCTTTTCCTC AAGTGGCTCT 
TCCATGTCAT CCTCTCAGAA CCAGCACGGG AGTTCCAAAG GGAAATCCCC CAGCAGAAAT 
AAGAAGCCGT CCTTAACAGC CGTCATAGAT AAACTGAAGC ATGGGGTTGT CACCAGTGGC 
CCTGGAGGTG AAGACCCGAT GGATGGCCAG ATGGGAGTGA GCACAAATTC TTCTAGCCAT 
CCTATGTCCT CCAAACATAA CATGTCAGGA GGAGAGTTCC AGGGTAAGCG TGAGAAAAGT 
GATAAAGAAA AATCCAAGGT TTCCACCTCG GGGGGTTCAG TGGATTCGTC TAAGAAGACC 
TCAGAGTCAA AAAATGTGGG GAGCACAGGT GTGGCAAAAA TTATCATCAG CAAGCATGAT 
GGGGGTTCCC CCAGCATTAA AGCCAAAGTG ACTTTGCAGA AACCTGGGGA AAGTAGTGGA 
GAAGGGCTCA GACCTCAGAT GGCCTCTTCC AAAAACTATG GCTCTCCACT CATCAGTGGT 
TCCACTCCAA AGCATGAGCG TGGCTCTCCT AGCCATAGTA AGTCACCAGC ATATACCCCC 
CAGAATCTGG ACAGTGAAAG TGAGTCAGGC TCCTCCATAG CAGAGAAATC TTATCAGAAC 
AGTCCGAGCT CAGATGATGG TATCCGACCT CTTCCAGAAT ATAGCACAGA AAAGCATAAG 
AAGCACAAAA AAGAAAAGAA GAAAGTAAAA GACAAAGATA GAGACCGGGA CCGGGACAAA 
GACCGGGACA AGAAAAAATC TCATAGCATC AAGCCAGAGA GTTGGTCTAA ATCACCCATC 
TCTTCAGACC AGTCCTTGTC TATGACAAGC AACACAATTT TATCTACAGA CAGGCCCTCA 
AGGCTCAGCC CTGACTTTAT GATTGGGGAG GAAGATGATG ATCTTATGGA TGTGGCCCTG 
ATTGGCAATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_864009.2
CDS	215..4966
Translation	MKAQGETEESEKLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAVMYWKATNAGPLDKILHGSVGYLTPRSGGHLMNLKYYASPSDLLDDKTTSPIILHENNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYVPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEICPGSNKTKKKKSSRLPPDKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTNDDCPPIGTPVRDSSSSGHSQSALFDPDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNSQADTVDFSIIAVAGKALGPADLMEHHSGSQSPLLTTGDLGKEKTQKRVKEGNGTSNSSLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSSSASNSGKMKSSKSEGSSSSKLSSSVYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSGSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGSSSSTGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPMDGQMGVSTNSSSHPMSSKHNMSGGEFQGKREKSDKEKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSTDRPSRLSPDFMIGEEDDDLMDVALIGN

Target ORF information:

RefSeq Version	XM_858916.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_858916.4

ORF Insert Sequence:

ATGAAGGCTC AGGGGGAAAC CGAGGAATCG GAGAAGCTGA GTAAGATGAG TTCTCTCTTG 
GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCTTGGA GTGAAACCAT TAAGCTTGTG 
CGTCAAGTCA TGGAGAAGAG AGTTGTGATG AGTTCTGGAG GGCACCAACA TTTGGTCAGC 
TGTTTGGAGA CTTTGCAGAA AGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGACCGC 
TTGGAGTCTA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA 
TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT 
TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCAGA ACTTGTACAG 
CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGTCT TGTTAATCTT 
TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA 
GAACAGGATC TATCTAAAAT GGCAGTTATG TATTGGAAAG CAACCAATGC TGGTCCCTTG 
GATAAGATTC TTCATGGAAG TGTTGGCTAT CTTACTCCAA GAAGTGGGGG TCATTTAATG 
AACTTGAAGT ACTATGCCTC TCCTTCTGAC CTGCTGGATG ATAAGACTAC ATCTCCCATC 
ATTTTGCATG AGAATAATGT TCCTCGATCA TTGGGCATGA ACGCATCAGT GACAATTGAA 
GGAACATCTG CTATGTATAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT 
GACAACAAAT GGACCCCCTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT 
GCCTGTTTCT TCTTGAAATT TCCCCAACCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA 
CTTCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGT ACCCCTCTAT 
GAACTGATCA CTCAGTTTGA GCTGTCAAAG GACCCTGACC CCATACCTTT GAATCACAAC 
ATGAGATTTT ATGCTGCTCT TCCAGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT 
CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTAGTTA GCAAAATCAC CTTTCAGCAC 
CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACATC AAGTGGCCTA TAACACCCTA 
ATAGGAAGTT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT CCTCCAATTT 
GAAGTGTGTC CTCTCTCAGA GTCCCGTTTT AGCGTATCTT TTCAGCACCC TGTGAATGAC 
TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTGTAC 
AAGGGGCTGT CAGATGCACT CATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA 
TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAGGCC 
GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG 
CCCCCGGCTA GCAGCCCAGG GTATGGCATG ACCACAGGCA ACAACCCAAT GAGTGGTACC 
ACTACACCAA CCAACACCTT TCCGGGGGGT CCCATTACCA CCTTGTTTAA TATGAGCATG 
AGCATCAAAG ATCGGCATGA GTCGGTGGGC CATGGGGAGG ACTTCAGCAA GGTGTCTCAG 
AACCCAATTC TTACCAGTTT GTTGCAAATC ACAGGGAACG GGGGGTCTAC CATTGGCTCG 
AGTCCGACCC CTCCTCATCA CACGCCGCCA CCTGTCTCTT CGATGGCCGG CAACACCAAG 
AACCACCCGA TGCTCATGAA CCTTCTTAAA GATAACCCTG CCCAGGATTT CTCAACCCTT 
TATGGAAGCA GCCCTTTAGA AAGGCAGAAC TCTTCTTCCG GCTCACCCCG GATGGAAATA 
TGCCCGGGAA GCAACAAGAC AAAGAAGAAG AAGTCATCAA GATTACCACC TGACAAACCC 
AAGCACCAGA CTGAAGATGA CTTTCAGAGG GAGCTATTTT CAATGGATGT TGACTCACAG 
AACCCTATCT TTGATGTCAA CATGACAGCA GACACGCTGG ATACGCCACA CATCACTCCA 
GCTCCAAGCC AGTGTAGTAC TCCCCCAACA ACTTACCCGC AACCAGTACC TCACCCCCAA 
CCCAGTATTC AAAGGATGGT CCGACTATCC AGTTCAGACA GCATTGGCCC AGATGTAACT 
GATATCCTTT CAGACATTGC AGAAGAAGCC TCTAAGCTTC CCAGCACTAA TGACGATTGT 
CCACCCATTG GCACCCCTGT TCGAGATTCT TCAAGCTCTG GGCATTCTCA GAGTGCTCTC 
TTTGACCCTG ATGTCTTTCA AACCAATAAT AATGAAAATC CATACACAGA TCCAGCTGAC 
CTAATTGCAG ATGCTGCTGG TAGCCCTAGT AGTGACTCTC CTACAAATCA TTTTTTTCCT 
GATGGAGTAG ATTTTAATCC TGATTTGTTG AACAGCCAGA GCCAAAGTGG TTTTGGAGAA 
GAATATTTTG ATGAAAGCAG CCAAAGCGGA GATAATGATG ATTTCAAAGG ATTTGCATCT 
CAGGCACTAA ATACTTTGGG GGTGCCAATG CTTGGAGGTG ATAATGGGGA GACTAAGTTT 
AAAGGCAATA GCCAAGCTGA CACAGTTGAT TTCAGTATTA TAGCAGTGGC TGGTAAGGCT 
TTAGGTCCTG CAGATCTTAT GGAGCATCAC AGTGGTAGCC AGAGTCCTTT ATTGACTACT 
GGGGACTTAG GGAAAGAAAA GACTCAAAAA CGAGTAAAGG AAGGCAATGG CACCAGTAAC 
AGTAGTCTGT CAGGGCCAGG ATTAGATAGC AAACCAGGGA AGCGTAGTCG GACCCCTTCT 
AATGATGGCA AAAGCAAAGA TAAGCCTCCA AAGCGGAAGA AGGCAGACAC TGAAGGAAAG 
TCTCCATCTC ACAGTTCTTC TAACCGACCT TTCACTCCAC CTACCAGTAC GGGTGGATCC 
AAATCACCAG GCAGTTCAGG AAGATCACAG ACTCCCCCAG GTGTTGCCAC ACCACCTATT 
CCCAAAATCA CTATTCAGAT TCCTAAAGGT ACCGTGATGG TGGGCAAGCC CTCATCCCAC 
AGTCAGTATA CCAGCAGTGG TTCTGTGTCT TCCTCAGGCA GTAAAAGCCA CCATAGCCAT 
TCTTCCTCCT CTTCTTCCTC CTCTTCTGCT TCCAACTCAG GCAAGATGAA AAGCAGTAAA 
TCAGAAGGTT CATCAAGTTC CAAGTTAAGT AGCAGTGTAT ATTCTAGCCA AGGGTCTTCT 
GGATCTAGTC AGTCCAAAAA TTCATCCCAG TCTGGGGGGA AGCCAGGCTC CTCTCCCATT 
ACCAAGCATG GACTGAGCAG TGGCTCCGGC AGCACCAAGA TGAAACCTCA AGGGAAGCCA 
TCATCACTTA TGAATCCTTC TTTAAGTAAA CCAAACATAT CCCCTTCTCA TTCAAGGCCA 
CCTGGAGGCT CTGATAAGCT TGCCTCTCCA ATGAAGCCTG TTCCTGGGAC TCCCCCATCT 
TCTAAAGCCA AGTCCCCCAT CAGTTCAGGT TCTGGTGGTT CTCATATGTC TGGAAGTAGT 
TCAAGCACTG GCATGAAGTC ATCTTCAGGG TTAGGATCTT CAGGCTCATT GTCTCAGAAA 
ACTCCCCCAT CATCTAATTC TTGTACAGCA TCTTCCTCTT CCTTTTCCTC AAGTGGCTCT 
TCCATGTCAT CCTCTCAGAA CCAGCACGGG AGTTCCAAAG GGAAATCCCC CAGCAGAAAT 
AAGAAGCCGT CCTTAACAGC CGTCATAGAT AAACTGAAGC ATGGGGTTGT CACCAGTGGC 
CCTGGAGGTG AAGACCCGAT GGATGGCCAG ATGGGAGTGA GCACAAATTC TTCTAGCCAT 
CCTATGTCCT CCAAACATAA CATGTCAGGA GGAGAGTTCC AGGGTAAGCG TGAGAAAAGT 
GATAAAGAAA AATCCAAGGT TTCCACCTCG GGGGGTTCAG TGGATTCGTC TAAGAAGACC 
TCAGAGTCAA AAAATGTGGG GAGCACAGGT GTGGCAAAAA TTATCATCAG CAAGCATGAT 
GGGGGTTCCC CCAGCATTAA AGCCAAAGTG ACTTTGCAGA AACCTGGGGA AAGTAGTGGA 
GAAGGGCTCA GACCTCAGAT GGCCTCTTCC AAAAACTATG GCTCTCCACT CATCAGTGGT 
TCCACTCCAA AGCATGAGCG TGGCTCTCCT AGCCATAGTA AGTCACCAGC ATATACCCCC 
CAGAATCTGG ACAGTGAAAG TGAGTCAGGC TCCTCCATAG CAGAGAAATC TTATCAGAAC 
AGTCCGAGCT CAGATGATGG TATCCGACCT CTTCCAGAAT ATAGCACAGA AAAGCATAAG 
AAGCACAAAA AAGAAAAGAA GAAAGTAAAA GACAAAGATA GAGACCGGGA CCGGGACAAA 
GACCGGGACA AGAAAAAATC TCATAGCATC AAGCCAGAGA GTTGGTCTAA ATCACCCATC 
TCTTCAGACC AGTCCTTGTC TATGACAAGC AACACAATTT TATCTACAGA CAGGCCCTCA 
AGGCTCAGCC CTGACTTTAT GATTGGGGAG GAAGATGATG ATCTTATGGA TGTGGCCCTG 
ATTGGCAATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED1 ( XM_858916.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_864009.2 Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X1, mRNA.

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CloneID	OCe11780
Clone ID Related Accession (Same CDS sequence)	XM_858916.4 , XM_858916.5
Accession Version	XM_858916.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mediator of RNA polymerase II transcription subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_858916.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCTC AGGGGGAAAC CGAGGAATCG GAGAAGCTGA GTAAGATGAG TTCTCTCTTG 
GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCTTGGA GTGAAACCAT TAAGCTTGTG 
CGTCAAGTCA TGGAGAAGAG AGTTGTGATG AGTTCTGGAG GGCACCAACA TTTGGTCAGC 
TGTTTGGAGA CTTTGCAGAA AGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGACCGC 
TTGGAGTCTA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA 
TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT 
TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCAGA ACTTGTACAG 
CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGTCT TGTTAATCTT 
TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA 
GAACAGGATC TATCTAAAAT GGCAGTTATG TATTGGAAAG CAACCAATGC TGGTCCCTTG 
GATAAGATTC TTCATGGAAG TGTTGGCTAT CTTACTCCAA GAAGTGGGGG TCATTTAATG 
AACTTGAAGT ACTATGCCTC TCCTTCTGAC CTGCTGGATG ATAAGACTAC ATCTCCCATC 
ATTTTGCATG AGAATAATGT TCCTCGATCA TTGGGCATGA ACGCATCAGT GACAATTGAA 
GGAACATCTG CTATGTATAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT 
GACAACAAAT GGACCCCCTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT 
GCCTGTTTCT TCTTGAAATT TCCCCAACCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA 
CTTCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGT ACCCCTCTAT 
GAACTGATCA CTCAGTTTGA GCTGTCAAAG GACCCTGACC CCATACCTTT GAATCACAAC 
ATGAGATTTT ATGCTGCTCT TCCAGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT 
CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTAGTTA GCAAAATCAC CTTTCAGCAC 
CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACATC AAGTGGCCTA TAACACCCTA 
ATAGGAAGTT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT CCTCCAATTT 
GAAGTGTGTC CTCTCTCAGA GTCCCGTTTT AGCGTATCTT TTCAGCACCC TGTGAATGAC 
TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTGTAC 
AAGGGGCTGT CAGATGCACT CATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA 
TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAGGCC 
GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG 
CCCCCGGCTA GCAGCCCAGG GTATGGCATG ACCACAGGCA ACAACCCAAT GAGTGGTACC 
ACTACACCAA CCAACACCTT TCCGGGGGGT CCCATTACCA CCTTGTTTAA TATGAGCATG 
AGCATCAAAG ATCGGCATGA GTCGGTGGGC CATGGGGAGG ACTTCAGCAA GGTGTCTCAG 
AACCCAATTC TTACCAGTTT GTTGCAAATC ACAGGGAACG GGGGGTCTAC CATTGGCTCG 
AGTCCGACCC CTCCTCATCA CACGCCGCCA CCTGTCTCTT CGATGGCCGG CAACACCAAG 
AACCACCCGA TGCTCATGAA CCTTCTTAAA GATAACCCTG CCCAGGATTT CTCAACCCTT 
TATGGAAGCA GCCCTTTAGA AAGGCAGAAC TCTTCTTCCG GCTCACCCCG GATGGAAATA 
TGCCCGGGAA GCAACAAGAC AAAGAAGAAG AAGTCATCAA GATTACCACC TGACAAACCC 
AAGCACCAGA CTGAAGATGA CTTTCAGAGG GAGCTATTTT CAATGGATGT TGACTCACAG 
AACCCTATCT TTGATGTCAA CATGACAGCA GACACGCTGG ATACGCCACA CATCACTCCA 
GCTCCAAGCC AGTGTAGTAC TCCCCCAACA ACTTACCCGC AACCAGTACC TCACCCCCAA 
CCCAGTATTC AAAGGATGGT CCGACTATCC AGTTCAGACA GCATTGGCCC AGATGTAACT 
GATATCCTTT CAGACATTGC AGAAGAAGCC TCTAAGCTTC CCAGCACTAA TGACGATTGT 
CCACCCATTG GCACCCCTGT TCGAGATTCT TCAAGCTCTG GGCATTCTCA GAGTGCTCTC 
TTTGACCCTG ATGTCTTTCA AACCAATAAT AATGAAAATC CATACACAGA TCCAGCTGAC 
CTAATTGCAG ATGCTGCTGG TAGCCCTAGT AGTGACTCTC CTACAAATCA TTTTTTTCCT 
GATGGAGTAG ATTTTAATCC TGATTTGTTG AACAGCCAGA GCCAAAGTGG TTTTGGAGAA 
GAATATTTTG ATGAAAGCAG CCAAAGCGGA GATAATGATG ATTTCAAAGG ATTTGCATCT 
CAGGCACTAA ATACTTTGGG GGTGCCAATG CTTGGAGGTG ATAATGGGGA GACTAAGTTT 
AAAGGCAATA GCCAAGCTGA CACAGTTGAT TTCAGTATTA TAGCAGTGGC TGGTAAGGCT 
TTAGGTCCTG CAGATCTTAT GGAGCATCAC AGTGGTAGCC AGAGTCCTTT ATTGACTACT 
GGGGACTTAG GGAAAGAAAA GACTCAAAAA CGAGTAAAGG AAGGCAATGG CACCAGTAAC 
AGTAGTCTGT CAGGGCCAGG ATTAGATAGC AAACCAGGGA AGCGTAGTCG GACCCCTTCT 
AATGATGGCA AAAGCAAAGA TAAGCCTCCA AAGCGGAAGA AGGCAGACAC TGAAGGAAAG 
TCTCCATCTC ACAGTTCTTC TAACCGACCT TTCACTCCAC CTACCAGTAC GGGTGGATCC 
AAATCACCAG GCAGTTCAGG AAGATCACAG ACTCCCCCAG GTGTTGCCAC ACCACCTATT 
CCCAAAATCA CTATTCAGAT TCCTAAAGGT ACCGTGATGG TGGGCAAGCC CTCATCCCAC 
AGTCAGTATA CCAGCAGTGG TTCTGTGTCT TCCTCAGGCA GTAAAAGCCA CCATAGCCAT 
TCTTCCTCCT CTTCTTCCTC CTCTTCTGCT TCCAACTCAG GCAAGATGAA AAGCAGTAAA 
TCAGAAGGTT CATCAAGTTC CAAGTTAAGT AGCAGTGTAT ATTCTAGCCA AGGGTCTTCT 
GGATCTAGTC AGTCCAAAAA TTCATCCCAG TCTGGGGGGA AGCCAGGCTC CTCTCCCATT 
ACCAAGCATG GACTGAGCAG TGGCTCCGGC AGCACCAAGA TGAAACCTCA AGGGAAGCCA 
TCATCACTTA TGAATCCTTC TTTAAGTAAA CCAAACATAT CCCCTTCTCA TTCAAGGCCA 
CCTGGAGGCT CTGATAAGCT TGCCTCTCCA ATGAAGCCTG TTCCTGGGAC TCCCCCATCT 
TCTAAAGCCA AGTCCCCCAT CAGTTCAGGT TCTGGTGGTT CTCATATGTC TGGAAGTAGT 
TCAAGCACTG GCATGAAGTC ATCTTCAGGG TTAGGATCTT CAGGCTCATT GTCTCAGAAA 
ACTCCCCCAT CATCTAATTC TTGTACAGCA TCTTCCTCTT CCTTTTCCTC AAGTGGCTCT 
TCCATGTCAT CCTCTCAGAA CCAGCACGGG AGTTCCAAAG GGAAATCCCC CAGCAGAAAT 
AAGAAGCCGT CCTTAACAGC CGTCATAGAT AAACTGAAGC ATGGGGTTGT CACCAGTGGC 
CCTGGAGGTG AAGACCCGAT GGATGGCCAG ATGGGAGTGA GCACAAATTC TTCTAGCCAT 
CCTATGTCCT CCAAACATAA CATGTCAGGA GGAGAGTTCC AGGGTAAGCG TGAGAAAAGT 
GATAAAGAAA AATCCAAGGT TTCCACCTCG GGGGGTTCAG TGGATTCGTC TAAGAAGACC 
TCAGAGTCAA AAAATGTGGG GAGCACAGGT GTGGCAAAAA TTATCATCAG CAAGCATGAT 
GGGGGTTCCC CCAGCATTAA AGCCAAAGTG ACTTTGCAGA AACCTGGGGA AAGTAGTGGA 
GAAGGGCTCA GACCTCAGAT GGCCTCTTCC AAAAACTATG GCTCTCCACT CATCAGTGGT 
TCCACTCCAA AGCATGAGCG TGGCTCTCCT AGCCATAGTA AGTCACCAGC ATATACCCCC 
CAGAATCTGG ACAGTGAAAG TGAGTCAGGC TCCTCCATAG CAGAGAAATC TTATCAGAAC 
AGTCCGAGCT CAGATGATGG TATCCGACCT CTTCCAGAAT ATAGCACAGA AAAGCATAAG 
AAGCACAAAA AAGAAAAGAA GAAAGTAAAA GACAAAGATA GAGACCGGGA CCGGGACAAA 
GACCGGGACA AGAAAAAATC TCATAGCATC AAGCCAGAGA GTTGGTCTAA ATCACCCATC 
TCTTCAGACC AGTCCTTGTC TATGACAAGC AACACAATTT TATCTACAGA CAGGCCCTCA 
AGGCTCAGCC CTGACTTTAT GATTGGGGAG GAAGATGATG ATCTTATGGA TGTGGCCCTG 
ATTGGCAATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_864009.2
CDS	223..4974
Translation	MKAQGETEESEKLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAVMYWKATNAGPLDKILHGSVGYLTPRSGGHLMNLKYYASPSDLLDDKTTSPIILHENNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYVPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEICPGSNKTKKKKSSRLPPDKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTNDDCPPIGTPVRDSSSSGHSQSALFDPDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNSQADTVDFSIIAVAGKALGPADLMEHHSGSQSPLLTTGDLGKEKTQKRVKEGNGTSNSSLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSSSASNSGKMKSSKSEGSSSSKLSSSVYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSGSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGSSSSTGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPMDGQMGVSTNSSSHPMSSKHNMSGGEFQGKREKSDKEKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSTDRPSRLSPDFMIGEEDDDLMDVALIGN

Target ORF information:

RefSeq Version	XM_858916.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_858916.5

ORF Insert Sequence:

ATGAAGGCTC AGGGGGAAAC CGAGGAATCG GAGAAGCTGA GTAAGATGAG TTCTCTCTTG 
GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCTTGGA GTGAAACCAT TAAGCTTGTG 
CGTCAAGTCA TGGAGAAGAG AGTTGTGATG AGTTCTGGAG GGCACCAACA TTTGGTCAGC 
TGTTTGGAGA CTTTGCAGAA AGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGACCGC 
TTGGAGTCTA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA 
TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT 
TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCAGA ACTTGTACAG 
CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGTCT TGTTAATCTT 
TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA 
GAACAGGATC TATCTAAAAT GGCAGTTATG TATTGGAAAG CAACCAATGC TGGTCCCTTG 
GATAAGATTC TTCATGGAAG TGTTGGCTAT CTTACTCCAA GAAGTGGGGG TCATTTAATG 
AACTTGAAGT ACTATGCCTC TCCTTCTGAC CTGCTGGATG ATAAGACTAC ATCTCCCATC 
ATTTTGCATG AGAATAATGT TCCTCGATCA TTGGGCATGA ACGCATCAGT GACAATTGAA 
GGAACATCTG CTATGTATAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT 
GACAACAAAT GGACCCCCTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT 
GCCTGTTTCT TCTTGAAATT TCCCCAACCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA 
CTTCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGT ACCCCTCTAT 
GAACTGATCA CTCAGTTTGA GCTGTCAAAG GACCCTGACC CCATACCTTT GAATCACAAC 
ATGAGATTTT ATGCTGCTCT TCCAGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT 
CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTAGTTA GCAAAATCAC CTTTCAGCAC 
CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACATC AAGTGGCCTA TAACACCCTA 
ATAGGAAGTT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT CCTCCAATTT 
GAAGTGTGTC CTCTCTCAGA GTCCCGTTTT AGCGTATCTT TTCAGCACCC TGTGAATGAC 
TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTGTAC 
AAGGGGCTGT CAGATGCACT CATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA 
TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAGGCC 
GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG 
CCCCCGGCTA GCAGCCCAGG GTATGGCATG ACCACAGGCA ACAACCCAAT GAGTGGTACC 
ACTACACCAA CCAACACCTT TCCGGGGGGT CCCATTACCA CCTTGTTTAA TATGAGCATG 
AGCATCAAAG ATCGGCATGA GTCGGTGGGC CATGGGGAGG ACTTCAGCAA GGTGTCTCAG 
AACCCAATTC TTACCAGTTT GTTGCAAATC ACAGGGAACG GGGGGTCTAC CATTGGCTCG 
AGTCCGACCC CTCCTCATCA CACGCCGCCA CCTGTCTCTT CGATGGCCGG CAACACCAAG 
AACCACCCGA TGCTCATGAA CCTTCTTAAA GATAACCCTG CCCAGGATTT CTCAACCCTT 
TATGGAAGCA GCCCTTTAGA AAGGCAGAAC TCTTCTTCCG GCTCACCCCG GATGGAAATA 
TGCCCGGGAA GCAACAAGAC AAAGAAGAAG AAGTCATCAA GATTACCACC TGACAAACCC 
AAGCACCAGA CTGAAGATGA CTTTCAGAGG GAGCTATTTT CAATGGATGT TGACTCACAG 
AACCCTATCT TTGATGTCAA CATGACAGCA GACACGCTGG ATACGCCACA CATCACTCCA 
GCTCCAAGCC AGTGTAGTAC TCCCCCAACA ACTTACCCGC AACCAGTACC TCACCCCCAA 
CCCAGTATTC AAAGGATGGT CCGACTATCC AGTTCAGACA GCATTGGCCC AGATGTAACT 
GATATCCTTT CAGACATTGC AGAAGAAGCC TCTAAGCTTC CCAGCACTAA TGACGATTGT 
CCACCCATTG GCACCCCTGT TCGAGATTCT TCAAGCTCTG GGCATTCTCA GAGTGCTCTC 
TTTGACCCTG ATGTCTTTCA AACCAATAAT AATGAAAATC CATACACAGA TCCAGCTGAC 
CTAATTGCAG ATGCTGCTGG TAGCCCTAGT AGTGACTCTC CTACAAATCA TTTTTTTCCT 
GATGGAGTAG ATTTTAATCC TGATTTGTTG AACAGCCAGA GCCAAAGTGG TTTTGGAGAA 
GAATATTTTG ATGAAAGCAG CCAAAGCGGA GATAATGATG ATTTCAAAGG ATTTGCATCT 
CAGGCACTAA ATACTTTGGG GGTGCCAATG CTTGGAGGTG ATAATGGGGA GACTAAGTTT 
AAAGGCAATA GCCAAGCTGA CACAGTTGAT TTCAGTATTA TAGCAGTGGC TGGTAAGGCT 
TTAGGTCCTG CAGATCTTAT GGAGCATCAC AGTGGTAGCC AGAGTCCTTT ATTGACTACT 
GGGGACTTAG GGAAAGAAAA GACTCAAAAA CGAGTAAAGG AAGGCAATGG CACCAGTAAC 
AGTAGTCTGT CAGGGCCAGG ATTAGATAGC AAACCAGGGA AGCGTAGTCG GACCCCTTCT 
AATGATGGCA AAAGCAAAGA TAAGCCTCCA AAGCGGAAGA AGGCAGACAC TGAAGGAAAG 
TCTCCATCTC ACAGTTCTTC TAACCGACCT TTCACTCCAC CTACCAGTAC GGGTGGATCC 
AAATCACCAG GCAGTTCAGG AAGATCACAG ACTCCCCCAG GTGTTGCCAC ACCACCTATT 
CCCAAAATCA CTATTCAGAT TCCTAAAGGT ACCGTGATGG TGGGCAAGCC CTCATCCCAC 
AGTCAGTATA CCAGCAGTGG TTCTGTGTCT TCCTCAGGCA GTAAAAGCCA CCATAGCCAT 
TCTTCCTCCT CTTCTTCCTC CTCTTCTGCT TCCAACTCAG GCAAGATGAA AAGCAGTAAA 
TCAGAAGGTT CATCAAGTTC CAAGTTAAGT AGCAGTGTAT ATTCTAGCCA AGGGTCTTCT 
GGATCTAGTC AGTCCAAAAA TTCATCCCAG TCTGGGGGGA AGCCAGGCTC CTCTCCCATT 
ACCAAGCATG GACTGAGCAG TGGCTCCGGC AGCACCAAGA TGAAACCTCA AGGGAAGCCA 
TCATCACTTA TGAATCCTTC TTTAAGTAAA CCAAACATAT CCCCTTCTCA TTCAAGGCCA 
CCTGGAGGCT CTGATAAGCT TGCCTCTCCA ATGAAGCCTG TTCCTGGGAC TCCCCCATCT 
TCTAAAGCCA AGTCCCCCAT CAGTTCAGGT TCTGGTGGTT CTCATATGTC TGGAAGTAGT 
TCAAGCACTG GCATGAAGTC ATCTTCAGGG TTAGGATCTT CAGGCTCATT GTCTCAGAAA 
ACTCCCCCAT CATCTAATTC TTGTACAGCA TCTTCCTCTT CCTTTTCCTC AAGTGGCTCT 
TCCATGTCAT CCTCTCAGAA CCAGCACGGG AGTTCCAAAG GGAAATCCCC CAGCAGAAAT 
AAGAAGCCGT CCTTAACAGC CGTCATAGAT AAACTGAAGC ATGGGGTTGT CACCAGTGGC 
CCTGGAGGTG AAGACCCGAT GGATGGCCAG ATGGGAGTGA GCACAAATTC TTCTAGCCAT 
CCTATGTCCT CCAAACATAA CATGTCAGGA GGAGAGTTCC AGGGTAAGCG TGAGAAAAGT 
GATAAAGAAA AATCCAAGGT TTCCACCTCG GGGGGTTCAG TGGATTCGTC TAAGAAGACC 
TCAGAGTCAA AAAATGTGGG GAGCACAGGT GTGGCAAAAA TTATCATCAG CAAGCATGAT 
GGGGGTTCCC CCAGCATTAA AGCCAAAGTG ACTTTGCAGA AACCTGGGGA AAGTAGTGGA 
GAAGGGCTCA GACCTCAGAT GGCCTCTTCC AAAAACTATG GCTCTCCACT CATCAGTGGT 
TCCACTCCAA AGCATGAGCG TGGCTCTCCT AGCCATAGTA AGTCACCAGC ATATACCCCC 
CAGAATCTGG ACAGTGAAAG TGAGTCAGGC TCCTCCATAG CAGAGAAATC TTATCAGAAC 
AGTCCGAGCT CAGATGATGG TATCCGACCT CTTCCAGAAT ATAGCACAGA AAAGCATAAG 
AAGCACAAAA AAGAAAAGAA GAAAGTAAAA GACAAAGATA GAGACCGGGA CCGGGACAAA 
GACCGGGACA AGAAAAAATC TCATAGCATC AAGCCAGAGA GTTGGTCTAA ATCACCCATC 
TCTTCAGACC AGTCCTTGTC TATGACAAGC AACACAATTT TATCTACAGA CAGGCCCTCA 
AGGCTCAGCC CTGACTTTAT GATTGGGGAG GAAGATGATG ATCTTATGGA TGTGGCCCTG 
ATTGGCAATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

MED1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

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