MED1 cDNA ORF clone, Bos taurus(cattle)

The following MED1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa36555 NM_001205876.1
Latest version!
Bos taurus mediator complex subunit 1 (MED1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa50301 XM_024980169.1
Latest version!
Bos taurus mediator complex subunit 1 (MED1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa50301 XM_010816249.2
Latest version!
Bos taurus mediator complex subunit 1 (MED1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa36555
    Clone ID Related Accession (Same CDS sequence) NM_001205876.1
    Accession Version NM_001205876.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4728bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000019.1. On Apr 20, 2011 this sequence version replaced XM_598984.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571260.45192.1, SRR5721698.4055.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAGGCTC AGGGGGAGAC CGAGGAGTCG GAAAAGCTGA GTAAGATGAG TTCTCTCTTG 
    GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCTTGGA GTGAAACCAT TAAGCTTGTG
    CGTCAAGTCA TGGAGAAGAG GGTTGTAATG AGTTCTGGAG GGCACCAACA TTTGGTCAGC
    TGTTTGGAGA CATTGCAGAA AGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGACCGT
    TTGGAGTCTA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA
    TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT
    TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCAGA GCTTGTACAG
    CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGTCT TGTTAATCTT
    TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA
    GAACAGGATC TATCTAAAAT GGCAGTTATG TATTGGAAAG CAACCAATGC TGGTCCCTTG
    GACAAGATTC TTCATGGAAG TGTTGGCTAT CTCACTCCAA GAAGTGGGGG TCATTTAATG
    AACTTGAAGT ATTATGCCTC TCCTTCTGAC CTGCTGGATG ATAAGACAAC ATCCCCTATC
    ATTTTGCATG AGAATAATGT TCCTCGATCT TTGGGCATGA ATGCATCAGT GACAATCGAA
    GGAACATCTG CTATGTATAA ACTCCCAATT GCACCATTAA TTATGGGATC ACATCCAGTT
    GACAACAAAT GGACTCCCTC CTTCTCCTTA GTTACCAGTG CCAACAGTGT TGATCTTCCT
    GCTTGTTTCT TCTTGAAATT TCCCCAGCCA CTTCCAGTAT CTAGAGCATT TGTTCAGAAA
    ATTCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAGC CAACTTATGT ACCCCTCTAT
    GAACTGATCA CTCAATTTGA ACTGTCAAAG GATCCTGACC CCTTACCTTT GAATCACAAC
    ATGCGATTTT ATGCTGCTCT TCCAGGTCAA CAGCACTGCT ATTTCCTCAA CAAGGATGCT
    CCTCTTCCAG ATGGTAGAAG TCTACAGGGA ACCCTTGTTA GCAAAATCAC CTTTCAGCAC
    CCTGGCCGGG TTCCTCTTAT CCTGAATCTG ATCAGACACC AAGTGGCCTA TAACACCCTA
    ATAGGAAGCT GTGTCAAAAG AACTATTCTG AAGGAAGATT CTCCAGGGCT TCTCCAATTT
    GAAGTGTGTC CCCTCTCAGA GTCTCGTTTC AGCGTGTCTT TTCAGCACCC TGTGAATGAC
    TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTCTAC
    AAGGGGCTGT CAGATGCACT CATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA
    TGTATGTCGA TTCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAGGCC
    GACACTCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG
    CCCCCGGCTA GCAGCCCAGG GTATGGCATG ACCACAGGCA ACAACCCAAT GAGTGGTACC
    ACTACACCAA CCAACACCTT TCCGGGGGGT CCCATTACCA CCTTGTTTAA TATGAGCATG
    AGCATCAAAG ATCGGCATGA GTCGGTGGGC CATGGGGAGG ACTTCAGCAA GGTATCTCAG
    AACCCAATTC TTACCAGTTT GTTGCAAATC ACAGGGAACG GGGGGTCTAC CATTGGCTCG
    AGTCCGACCC CTCCTCATCA CACGCCGCCA CCTGTCTCTT CGATGGCCGG CAACACCAAG
    AACCACCCGA TGCTCATGAA CCTTCTTAAA GATAATCCTG CCCAGGATTT CTCAACCCTT
    TATGGAAGCA GCCCTTTAGA AAGGCAGAAC TCCTCTTCCG GCTCACCCCG GATGGAAATG
    TGCTCGGGAA GCAACAAGAC AAAGAAGAAG AAGTCATCAA GATTACCACC TGACAAACCA
    AAGCACCAGA CTGAAGATGA CTTTCAGAGG GAGCTATTTT CAATGGATGT TGACTCACAA
    AACCCTATCT TTGATGTCAA CATGACAGCT GACACGCTGG ATACGCCACA CATCACTCCA
    GCTCCAAGCC AGTGTAGTAC TCCCCCAACA ACTTACCCGC AACCAGTACC TCACCCCCAA
    CCCAGTATTC AAAGGATGGT CCGACTATCC AGTTCAGACA GCATTGGCCC AGATGTAACT
    GATATCCTTT CAGACATTGC AGAAGAAGCT TCTAAGCTTC CCAGCACTAG TGACGATTGT
    CCACCCATTG GCACCCCTGT TCGAGATTCT TCAAGCTCTG GGCATTCTCA GAGTGCCCTC
    TTTGACCCTG ATGTCTTTCA AGCCAATAAT AATGAAAATC CATACACTGA TCCAGCTGAC
    CTTATTGCAG ATGCTGCTGG CAGCCCCAGT AGTGACTCAC CTACCAATCA TTTTTTTCCT
    GATGGAGTAG ATTTCAATCC TGATTTGTTG AACAGCCAGA GCCAAAGTGG TTTTGGAGAA
    GAATATTTTG ATGAAAGCAG CCAAAGTGGA GATAATGATG ATTTCAAAGG ATTTGCATCT
    CAGGCACTAA ATACTTTGGG GGTGCCAATG CTTGGAGGTG ATAATGGGGA GACTAAGTTT
    AAAGGCAATA GCCAAGCTGA CACGGTTGAT TTCAGTATTA TAGCAGTGGC TGGTAAGGCT
    TTGGGTCCTA CAGATCTTAT GGAGCATCAC AGTGGTAGCC AGAGTCCTTT ATTGACCACT
    GGGGACTTAG GGAAAGAAAA GACTCAAAAG AGAGTAAAGG AAGGCAATGG TGCCAGTAAT
    AGTAGTCTGT CAGGGACAGG ATTAGACAGC AAACCAGGGA AGCGCAGTCG AACCCCTTCT
    AATGATGGCA AAAGCAAAGA TAAGCCTCCA AAGCGGAAGA AGGCAGACAC TGAGGGAAAG
    TCTCCATCTC ACAGTTCTAA CCGACCTTTC ACCCCACCTA CTAGTACAGG TGGATCCAAA
    TCTCCAGGCA GTTCAGGAAG ATCTCAGACT CCCCCAGGTG TTGCCACACC ACCCATTCCC
    AAAATTACTA TTCAGATTCC TAAGGGAACT GTGATGGTGG GCAAGCCCTC TTCTCACAGT
    CAGTATACCA GCAGTGGTTC TGTGTCTTCC TCAGGAAGTA AAAGCCACCA TAGCCATTCT
    TCCTTATCTT CTGCTTCCAA CTCAGGCAAG ATGAAAAGCA GTAAATCAGA AGGTTCATCA
    AGTTCTAAGT TAAGTAGCAG TATATATTCT AGCCAGGGGT CTTCTGGATC TAGCCAATCC
    AAAAATTCAT CCCAGTCTGG GGGAAAGCCA GGCTCCTCTC CTATTACCAA GCATGGACTG
    AGCAGTGGCT CCAGCAGCAC CAAGATGAAA CCTCAAGGGA AGCCATCATC ACTTATGAAT
    CCTTCTTTAA GTAAACCAAA CATATCCCCT TCCCATTCGA GGCCACCTGG AGGCTCTGAT
    AAGCTTGCCT CTCCAATGAA GCCTGTTCCT GGGACTCCCC CATCCTCTAA AGCCAAGTCC
    CCTATCAGTT CAGGTTCTGG TGGTTCTCAC ATGTCTGGAA CTAGTTCAAG CACCGGCATG
    AAGTCATCTT CAGGGTTAGG ATCCTCAGGC TCATTGTCTC AGAAAACTCC CCCATCATCT
    AACTCTTGTA CAGCATCTTC CTCTTCCTTT TCCTCAAGTG GCTCTTCCAT GTCATCCTCT
    CAGAACCAGC ATGGGAGTTC CAAAGGGAAA TCTCCCAGCA GAAATAAGAA GCCGTCTTTA
    ACAGCTGTCA TAGATAAACT GAAGCATGGG GTTGTCACCA GTGGCCCTGG GGGTGAAGAC
    CCAATGGACG GCCAAATGGG GGTGAGCACA AATTCTAGCC ATCCTGTGTC CTCCAAACAT
    AACATGTCTG GAGGAGAGTT CCAGGGCAAG CGTGAGAAAA GTGATAAAGA CAAATCAAAG
    GTTTCCACCT CGGGGGGCTC AGTGGATTCA TCTAAGAAGA CCTCAGAGTC TAAAAATGTG
    GGGAGCACGG GTGTGGCAAA AATTATCATC AGCAAGCATG ATGGGGGATC CCCCAGCATT
    AAAGCCAAAG TGACTTTGCA GAAACCTGGG GAAGGTGGTG GAGAAGGGCT CAGGCCTCAG
    ATGGCCTCTT CCAAAAACTA TGGCTCTCCA CTCATCAGTG GTTCCACTCC AAAGCATGAG
    CGTGGCTCTC CCAGCCATAG TAAGTCACCA GCATATACCC CCCAGAATCT GGACAGTGAA
    AGTGAGTCAG GCTCCTCCAT AGCAGAGAAA TCTTATCAGA ACAGTCCTAG CTCAGATGAT
    GGCATCCGAC CTCTTCCAGA ATACAGCACA GAGAAGCATA AGAAGCACAA AAAAGAAAAG
    AAGAAAGTAA AGGACAAAGA TAGGGACCGG GACAAAGACC GAGACAAGAA AAAATCTCAT
    AGCATCAAGC CAGAGAGTTG GTCTAAATCA CCCATCTCTT CAGACCAGTC CTTGTCTATG
    ACAAGTAACA CAATCTTATC TACAGACAGG CCCTCAAGGC TCAGCCCTGA CTTTATGATT
    GGGGAGGAAG ATGACGATCT TATGGATGTG GCCCTGATTG GCAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192805.1
    CDS185..4912
    Translation

    Target ORF information:

    RefSeq Version NM_001205876.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 1 (MED1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205876.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAGGCTC AGGGGGAGAC CGAGGAGTCG GAAAAGCTGA GTAAGATGAG TTCTCTCTTG 
    GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCTTGGA GTGAAACCAT TAAGCTTGTG
    CGTCAAGTCA TGGAGAAGAG GGTTGTAATG AGTTCTGGAG GGCACCAACA TTTGGTCAGC
    TGTTTGGAGA CATTGCAGAA AGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGACCGT
    TTGGAGTCTA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA
    TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT
    TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCAGA GCTTGTACAG
    CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGTCT TGTTAATCTT
    TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA
    GAACAGGATC TATCTAAAAT GGCAGTTATG TATTGGAAAG CAACCAATGC TGGTCCCTTG
    GACAAGATTC TTCATGGAAG TGTTGGCTAT CTCACTCCAA GAAGTGGGGG TCATTTAATG
    AACTTGAAGT ATTATGCCTC TCCTTCTGAC CTGCTGGATG ATAAGACAAC ATCCCCTATC
    ATTTTGCATG AGAATAATGT TCCTCGATCT TTGGGCATGA ATGCATCAGT GACAATCGAA
    GGAACATCTG CTATGTATAA ACTCCCAATT GCACCATTAA TTATGGGATC ACATCCAGTT
    GACAACAAAT GGACTCCCTC CTTCTCCTTA GTTACCAGTG CCAACAGTGT TGATCTTCCT
    GCTTGTTTCT TCTTGAAATT TCCCCAGCCA CTTCCAGTAT CTAGAGCATT TGTTCAGAAA
    ATTCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAGC CAACTTATGT ACCCCTCTAT
    GAACTGATCA CTCAATTTGA ACTGTCAAAG GATCCTGACC CCTTACCTTT GAATCACAAC
    ATGCGATTTT ATGCTGCTCT TCCAGGTCAA CAGCACTGCT ATTTCCTCAA CAAGGATGCT
    CCTCTTCCAG ATGGTAGAAG TCTACAGGGA ACCCTTGTTA GCAAAATCAC CTTTCAGCAC
    CCTGGCCGGG TTCCTCTTAT CCTGAATCTG ATCAGACACC AAGTGGCCTA TAACACCCTA
    ATAGGAAGCT GTGTCAAAAG AACTATTCTG AAGGAAGATT CTCCAGGGCT TCTCCAATTT
    GAAGTGTGTC CCCTCTCAGA GTCTCGTTTC AGCGTGTCTT TTCAGCACCC TGTGAATGAC
    TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTCTAC
    AAGGGGCTGT CAGATGCACT CATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA
    TGTATGTCGA TTCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAGGCC
    GACACTCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG
    CCCCCGGCTA GCAGCCCAGG GTATGGCATG ACCACAGGCA ACAACCCAAT GAGTGGTACC
    ACTACACCAA CCAACACCTT TCCGGGGGGT CCCATTACCA CCTTGTTTAA TATGAGCATG
    AGCATCAAAG ATCGGCATGA GTCGGTGGGC CATGGGGAGG ACTTCAGCAA GGTATCTCAG
    AACCCAATTC TTACCAGTTT GTTGCAAATC ACAGGGAACG GGGGGTCTAC CATTGGCTCG
    AGTCCGACCC CTCCTCATCA CACGCCGCCA CCTGTCTCTT CGATGGCCGG CAACACCAAG
    AACCACCCGA TGCTCATGAA CCTTCTTAAA GATAATCCTG CCCAGGATTT CTCAACCCTT
    TATGGAAGCA GCCCTTTAGA AAGGCAGAAC TCCTCTTCCG GCTCACCCCG GATGGAAATG
    TGCTCGGGAA GCAACAAGAC AAAGAAGAAG AAGTCATCAA GATTACCACC TGACAAACCA
    AAGCACCAGA CTGAAGATGA CTTTCAGAGG GAGCTATTTT CAATGGATGT TGACTCACAA
    AACCCTATCT TTGATGTCAA CATGACAGCT GACACGCTGG ATACGCCACA CATCACTCCA
    GCTCCAAGCC AGTGTAGTAC TCCCCCAACA ACTTACCCGC AACCAGTACC TCACCCCCAA
    CCCAGTATTC AAAGGATGGT CCGACTATCC AGTTCAGACA GCATTGGCCC AGATGTAACT
    GATATCCTTT CAGACATTGC AGAAGAAGCT TCTAAGCTTC CCAGCACTAG TGACGATTGT
    CCACCCATTG GCACCCCTGT TCGAGATTCT TCAAGCTCTG GGCATTCTCA GAGTGCCCTC
    TTTGACCCTG ATGTCTTTCA AGCCAATAAT AATGAAAATC CATACACTGA TCCAGCTGAC
    CTTATTGCAG ATGCTGCTGG CAGCCCCAGT AGTGACTCAC CTACCAATCA TTTTTTTCCT
    GATGGAGTAG ATTTCAATCC TGATTTGTTG AACAGCCAGA GCCAAAGTGG TTTTGGAGAA
    GAATATTTTG ATGAAAGCAG CCAAAGTGGA GATAATGATG ATTTCAAAGG ATTTGCATCT
    CAGGCACTAA ATACTTTGGG GGTGCCAATG CTTGGAGGTG ATAATGGGGA GACTAAGTTT
    AAAGGCAATA GCCAAGCTGA CACGGTTGAT TTCAGTATTA TAGCAGTGGC TGGTAAGGCT
    TTGGGTCCTA CAGATCTTAT GGAGCATCAC AGTGGTAGCC AGAGTCCTTT ATTGACCACT
    GGGGACTTAG GGAAAGAAAA GACTCAAAAG AGAGTAAAGG AAGGCAATGG TGCCAGTAAT
    AGTAGTCTGT CAGGGACAGG ATTAGACAGC AAACCAGGGA AGCGCAGTCG AACCCCTTCT
    AATGATGGCA AAAGCAAAGA TAAGCCTCCA AAGCGGAAGA AGGCAGACAC TGAGGGAAAG
    TCTCCATCTC ACAGTTCTAA CCGACCTTTC ACCCCACCTA CTAGTACAGG TGGATCCAAA
    TCTCCAGGCA GTTCAGGAAG ATCTCAGACT CCCCCAGGTG TTGCCACACC ACCCATTCCC
    AAAATTACTA TTCAGATTCC TAAGGGAACT GTGATGGTGG GCAAGCCCTC TTCTCACAGT
    CAGTATACCA GCAGTGGTTC TGTGTCTTCC TCAGGAAGTA AAAGCCACCA TAGCCATTCT
    TCCTTATCTT CTGCTTCCAA CTCAGGCAAG ATGAAAAGCA GTAAATCAGA AGGTTCATCA
    AGTTCTAAGT TAAGTAGCAG TATATATTCT AGCCAGGGGT CTTCTGGATC TAGCCAATCC
    AAAAATTCAT CCCAGTCTGG GGGAAAGCCA GGCTCCTCTC CTATTACCAA GCATGGACTG
    AGCAGTGGCT CCAGCAGCAC CAAGATGAAA CCTCAAGGGA AGCCATCATC ACTTATGAAT
    CCTTCTTTAA GTAAACCAAA CATATCCCCT TCCCATTCGA GGCCACCTGG AGGCTCTGAT
    AAGCTTGCCT CTCCAATGAA GCCTGTTCCT GGGACTCCCC CATCCTCTAA AGCCAAGTCC
    CCTATCAGTT CAGGTTCTGG TGGTTCTCAC ATGTCTGGAA CTAGTTCAAG CACCGGCATG
    AAGTCATCTT CAGGGTTAGG ATCCTCAGGC TCATTGTCTC AGAAAACTCC CCCATCATCT
    AACTCTTGTA CAGCATCTTC CTCTTCCTTT TCCTCAAGTG GCTCTTCCAT GTCATCCTCT
    CAGAACCAGC ATGGGAGTTC CAAAGGGAAA TCTCCCAGCA GAAATAAGAA GCCGTCTTTA
    ACAGCTGTCA TAGATAAACT GAAGCATGGG GTTGTCACCA GTGGCCCTGG GGGTGAAGAC
    CCAATGGACG GCCAAATGGG GGTGAGCACA AATTCTAGCC ATCCTGTGTC CTCCAAACAT
    AACATGTCTG GAGGAGAGTT CCAGGGCAAG CGTGAGAAAA GTGATAAAGA CAAATCAAAG
    GTTTCCACCT CGGGGGGCTC AGTGGATTCA TCTAAGAAGA CCTCAGAGTC TAAAAATGTG
    GGGAGCACGG GTGTGGCAAA AATTATCATC AGCAAGCATG ATGGGGGATC CCCCAGCATT
    AAAGCCAAAG TGACTTTGCA GAAACCTGGG GAAGGTGGTG GAGAAGGGCT CAGGCCTCAG
    ATGGCCTCTT CCAAAAACTA TGGCTCTCCA CTCATCAGTG GTTCCACTCC AAAGCATGAG
    CGTGGCTCTC CCAGCCATAG TAAGTCACCA GCATATACCC CCCAGAATCT GGACAGTGAA
    AGTGAGTCAG GCTCCTCCAT AGCAGAGAAA TCTTATCAGA ACAGTCCTAG CTCAGATGAT
    GGCATCCGAC CTCTTCCAGA ATACAGCACA GAGAAGCATA AGAAGCACAA AAAAGAAAAG
    AAGAAAGTAA AGGACAAAGA TAGGGACCGG GACAAAGACC GAGACAAGAA AAAATCTCAT
    AGCATCAAGC CAGAGAGTTG GTCTAAATCA CCCATCTCTT CAGACCAGTC CTTGTCTATG
    ACAAGTAACA CAATCTTATC TACAGACAGG CCCTCAAGGC TCAGCCCTGA CTTTATGATT
    GGGGAGGAAG ATGACGATCT TATGGATGTG GCCCTGATTG GCAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50301
    Clone ID Related Accession (Same CDS sequence) XM_010816249.2 , XM_024980169.1
    Accession Version XM_010816249.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4212bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104496.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816249.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTACTTGG CTCTCCAATC CTTAGAACAG GATCTATCTA AAATGGCAGT TATGTATTGG 
    AAAGCAACCA ATGCTGGTCC CTTGGACAAG ATTCTTCATG GAAGTGTTGG CTATCTCACT
    CCAAGAAGTG GGGGTCATTT AATGAACTTG AAGTATTATG CCTCTCCTTC TGACCTGCTG
    GATGATAAGA CAACATCCCC TATCATTTTG CATGAGAATA ATGTTCCTCG ATCTTTGGGC
    ATGAATGCAT CAGTGACAAT CGAAGGAACA TCTGCTATGT ATAAACTCCC AATTGCACCA
    TTAATTATGG GATCACATCC AGTTGACAAC AAATGGACTC CCTCCTTCTC CTTAGTTACC
    AGTGCCAACA GTGTTGATCT TCCTGCTTGT TTCTTCTTGA AATTTCCCCA GCCACTTCCA
    GTATCTAGAG CATTTGTTCA GAAAATTCAG AACTGCACAG GAATTCCATT GTTTGAAACT
    CAGCCAACTT ATGTACCCCT CTATGAACTG ATCACTCAAT TTGAACTGTC AAAGGATCCT
    GACCCCTTAC CTTTGAATCA CAACATGCGA TTTTATGCTG CTCTTCCAGG TCAACAGCAC
    TGCTATTTCC TCAACAAGGA TGCTCCTCTT CCAGATGGTA GAAGTCTACA GGGAACCCTT
    GTTAGCAAAA TCACCTTTCA GCACCCTGGC CGGGTTCCTC TTATCCTGAA TCTGATCAGA
    CACCAAGTGG CCTATAACAC CCTAATAGGA AGCTGTGTCA AAAGAACTAT TCTGAAGGAA
    GATTCTCCAG GGCTTCTCCA ATTTGAAGTG TGTCCCCTCT CAGAGTCTCG TTTCAGCGTG
    TCTTTTCAGC ACCCTGTGAA TGACTCCCTG GTGTGTGTGG TAATGGATGT GCAGGACTCA
    ACACATGTGA GCTGTAAACT CTACAAGGGG CTGTCAGATG CACTCATCTG CACAGATGAC
    TTCATTGCCA AAGTTGTTCA AAGATGTATG TCGATTCCTG TGACGATGAG GGCTATTCGG
    AGGAAAGCTG AAACCATTCA GGCCGACACT CCAGCACTGT CCCTCATTGC AGAGACAGTT
    GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA
    GGCAACAACC CAATGAGTGG TACCACTACA CCAACCAACA CCTTTCCGGG GGGTCCCATT
    ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG
    GAGGACTTCA GCAAGGTATC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG
    AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC
    TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAT
    CCTGCCCAGG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCCTCT
    TCCGGCTCAC CCCGGATGGA AATGTGCTCG GGAAGCAACA AGACAAAGAA GAAGAAGTCA
    TCAAGATTAC CACCTGACAA ACCAAAGCAC CAGACTGAAG ATGACTTTCA GAGGGAGCTA
    TTTTCAATGG ATGTTGACTC ACAAAACCCT ATCTTTGATG TCAACATGAC AGCTGACACG
    CTGGATACGC CACACATCAC TCCAGCTCCA AGCCAGTGTA GTACTCCCCC AACAACTTAC
    CCGCAACCAG TACCTCACCC CCAACCCAGT ATTCAAAGGA TGGTCCGACT ATCCAGTTCA
    GACAGCATTG GCCCAGATGT AACTGATATC CTTTCAGACA TTGCAGAAGA AGCTTCTAAG
    CTTCCCAGCA CTAGTGACGA TTGTCCACCC ATTGGCACCC CTGTTCGAGA TTCTTCAAGC
    TCTGGGCATT CTCAGAGTGC CCTCTTTGAC CCTGATGTCT TTCAAGCCAA TAATAATGAA
    AATCCATACA CTGATCCAGC TGACCTTATT GCAGATGCTG CTGGCAGCCC CAGTAGTGAC
    TCACCTACCA ATCATTTTTT TCCTGATGGA GTAGATTTCA ATCCTGATTT GTTGAACAGC
    CAGAGCCAAA GTGGTTTTGG AGAAGAATAT TTTGATGAAA GCAGCCAAAG TGGAGATAAT
    GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGGTGCC AATGCTTGGA
    GGTGATAATG GGGAGACTAA GTTTAAAGGC AATAGCCAAG CTGACACGGT TGATTTCAGT
    ATTATAGCAG TGGCTGGTAA GGCTTTGGGT CCTACAGATC TTATGGAGCA TCACAGTGGT
    AGCCAGAGTC CTTTATTGAC CACTGGGGAC TTAGGGAAAG AAAAGACTCA AAAGAGAGTA
    AAGGAAGGCA ATGGTGCCAG TAATAGTAGT CTGTCAGGGA CAGGATTAGA CAGCAAACCA
    GGGAAGCGCA GTCGAACCCC TTCTAATGAT GGCAAAAGCA AAGATAAGCC TCCAAAGCGG
    AAGAAGGCAG ACACTGAGGG AAAGTCTCCA TCTCACAGTT CTAACCGACC TTTCACCCCA
    CCTACTAGTA CAGGTGGATC CAAATCTCCA GGCAGTTCAG GAAGATCTCA GACTCCCCCA
    GGTGTTGCCA CACCACCCAT TCCCAAAATT ACTATTCAGA TTCCTAAGGG AACTGTGATG
    GTGGGCAAGC CCTCTTCTCA CAGTCAGTAT ACCAGCAGTG GTTCTGTGTC TTCCTCAGGA
    AGTAAAAGCC ACCATAGCCA TTCTTCCTTA TCTTCTGCTT CCAACTCAGG CAAGATGAAA
    AGCAGTAAAT CAGAAGGTTC ATCAAGTTCT AAGTTAAGTA GCAGTATATA TTCTAGCCAG
    GGGTCTTCTG GATCTAGCCA ATCCAAAAAT TCATCCCAGT CTGGGGGAAA GCCAGGCTCC
    TCTCCTATTA CCAAGCATGG ACTGAGCAGT GGCTCCAGCA GCACCAAGAT GAAACCTCAA
    GGGAAGCCAT CATCACTTAT GAATCCTTCT TTAAGTAAAC CAAACATATC CCCTTCCCAT
    TCGAGGCCAC CTGGAGGCTC TGATAAGCTT GCCTCTCCAA TGAAGCCTGT TCCTGGGACT
    CCCCCATCCT CTAAAGCCAA GTCCCCTATC AGTTCAGGTT CTGGTGGTTC TCACATGTCT
    GGAACTAGTT CAAGCACCGG CATGAAGTCA TCTTCAGGGT TAGGATCCTC AGGCTCATTG
    TCTCAGAAAA CTCCCCCATC ATCTAACTCT TGTACAGCAT CTTCCTCTTC CTTTTCCTCA
    AGTGGCTCTT CCATGTCATC CTCTCAGAAC CAGCATGGGA GTTCCAAAGG GAAATCTCCC
    AGCAGAAATA AGAAGCCGTC TTTAACAGCT GTCATAGATA AACTGAAGCA TGGGGTTGTC
    ACCAGTGGCC CTGGGGGTGA AGACCCAATG GACGGCCAAA TGGGGGTGAG CACAAATTCT
    AGCCATCCTG TGTCCTCCAA ACATAACATG TCTGGAGGAG AGTTCCAGGG CAAGCGTGAG
    AAAAGTGATA AAGACAAATC AAAGGTTTCC ACCTCGGGGG GCTCAGTGGA TTCATCTAAG
    AAGACCTCAG AGTCTAAAAA TGTGGGGAGC ACGGGTGTGG CAAAAATTAT CATCAGCAAG
    CATGATGGGG GATCCCCCAG CATTAAAGCC AAAGTGACTT TGCAGAAACC TGGGGAAGGT
    GGTGGAGAAG GGCTCAGGCC TCAGATGGCC TCTTCCAAAA ACTATGGCTC TCCACTCATC
    AGTGGTTCCA CTCCAAAGCA TGAGCGTGGC TCTCCCAGCC ATAGTAAGTC ACCAGCATAT
    ACCCCCCAGA ATCTGGACAG TGAAAGTGAG TCAGGCTCCT CCATAGCAGA GAAATCTTAT
    CAGAACAGTC CTAGCTCAGA TGATGGCATC CGACCTCTTC CAGAATACAG CACAGAGAAG
    CATAAGAAGC ACAAAAAAGA AAAGAAGAAA GTAAAGGACA AAGATAGGGA CCGGGACAAA
    GACCGAGACA AGAAAAAATC TCATAGCATC AAGCCAGAGA GTTGGTCTAA ATCACCCATC
    TCTTCAGACC AGTCCTTGTC TATGACAAGT AACACAATCT TATCTACAGA CAGGCCCTCA
    AGGCTCAGCC CTGACTTTAT GATTGGGGAG GAAGATGACG ATCTTATGGA TGTGGCCCTG
    ATTGGCAACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814551.1
    CDS207..4418
    Translation

    Target ORF information:

    RefSeq Version XM_010816249.2
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 1 (MED1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816249.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTACTTGG CTCTCCAATC CTTAGAACAG GATCTATCTA AAATGGCAGT TATGTATTGG 
    AAAGCAACCA ATGCTGGTCC CTTGGACAAG ATTCTTCATG GAAGTGTTGG CTATCTCACT
    CCAAGAAGTG GGGGTCATTT AATGAACTTG AAGTATTATG CCTCTCCTTC TGACCTGCTG
    GATGATAAGA CAACATCCCC TATCATTTTG CATGAGAATA ATGTTCCTCG ATCTTTGGGC
    ATGAATGCAT CAGTGACAAT CGAAGGAACA TCTGCTATGT ATAAACTCCC AATTGCACCA
    TTAATTATGG GATCACATCC AGTTGACAAC AAATGGACTC CCTCCTTCTC CTTAGTTACC
    AGTGCCAACA GTGTTGATCT TCCTGCTTGT TTCTTCTTGA AATTTCCCCA GCCACTTCCA
    GTATCTAGAG CATTTGTTCA GAAAATTCAG AACTGCACAG GAATTCCATT GTTTGAAACT
    CAGCCAACTT ATGTACCCCT CTATGAACTG ATCACTCAAT TTGAACTGTC AAAGGATCCT
    GACCCCTTAC CTTTGAATCA CAACATGCGA TTTTATGCTG CTCTTCCAGG TCAACAGCAC
    TGCTATTTCC TCAACAAGGA TGCTCCTCTT CCAGATGGTA GAAGTCTACA GGGAACCCTT
    GTTAGCAAAA TCACCTTTCA GCACCCTGGC CGGGTTCCTC TTATCCTGAA TCTGATCAGA
    CACCAAGTGG CCTATAACAC CCTAATAGGA AGCTGTGTCA AAAGAACTAT TCTGAAGGAA
    GATTCTCCAG GGCTTCTCCA ATTTGAAGTG TGTCCCCTCT CAGAGTCTCG TTTCAGCGTG
    TCTTTTCAGC ACCCTGTGAA TGACTCCCTG GTGTGTGTGG TAATGGATGT GCAGGACTCA
    ACACATGTGA GCTGTAAACT CTACAAGGGG CTGTCAGATG CACTCATCTG CACAGATGAC
    TTCATTGCCA AAGTTGTTCA AAGATGTATG TCGATTCCTG TGACGATGAG GGCTATTCGG
    AGGAAAGCTG AAACCATTCA GGCCGACACT CCAGCACTGT CCCTCATTGC AGAGACAGTT
    GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA
    GGCAACAACC CAATGAGTGG TACCACTACA CCAACCAACA CCTTTCCGGG GGGTCCCATT
    ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG
    GAGGACTTCA GCAAGGTATC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG
    AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC
    TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAT
    CCTGCCCAGG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCCTCT
    TCCGGCTCAC CCCGGATGGA AATGTGCTCG GGAAGCAACA AGACAAAGAA GAAGAAGTCA
    TCAAGATTAC CACCTGACAA ACCAAAGCAC CAGACTGAAG ATGACTTTCA GAGGGAGCTA
    TTTTCAATGG ATGTTGACTC ACAAAACCCT ATCTTTGATG TCAACATGAC AGCTGACACG
    CTGGATACGC CACACATCAC TCCAGCTCCA AGCCAGTGTA GTACTCCCCC AACAACTTAC
    CCGCAACCAG TACCTCACCC CCAACCCAGT ATTCAAAGGA TGGTCCGACT ATCCAGTTCA
    GACAGCATTG GCCCAGATGT AACTGATATC CTTTCAGACA TTGCAGAAGA AGCTTCTAAG
    CTTCCCAGCA CTAGTGACGA TTGTCCACCC ATTGGCACCC CTGTTCGAGA TTCTTCAAGC
    TCTGGGCATT CTCAGAGTGC CCTCTTTGAC CCTGATGTCT TTCAAGCCAA TAATAATGAA
    AATCCATACA CTGATCCAGC TGACCTTATT GCAGATGCTG CTGGCAGCCC CAGTAGTGAC
    TCACCTACCA ATCATTTTTT TCCTGATGGA GTAGATTTCA ATCCTGATTT GTTGAACAGC
    CAGAGCCAAA GTGGTTTTGG AGAAGAATAT TTTGATGAAA GCAGCCAAAG TGGAGATAAT
    GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGGTGCC AATGCTTGGA
    GGTGATAATG GGGAGACTAA GTTTAAAGGC AATAGCCAAG CTGACACGGT TGATTTCAGT
    ATTATAGCAG TGGCTGGTAA GGCTTTGGGT CCTACAGATC TTATGGAGCA TCACAGTGGT
    AGCCAGAGTC CTTTATTGAC CACTGGGGAC TTAGGGAAAG AAAAGACTCA AAAGAGAGTA
    AAGGAAGGCA ATGGTGCCAG TAATAGTAGT CTGTCAGGGA CAGGATTAGA CAGCAAACCA
    GGGAAGCGCA GTCGAACCCC TTCTAATGAT GGCAAAAGCA AAGATAAGCC TCCAAAGCGG
    AAGAAGGCAG ACACTGAGGG AAAGTCTCCA TCTCACAGTT CTAACCGACC TTTCACCCCA
    CCTACTAGTA CAGGTGGATC CAAATCTCCA GGCAGTTCAG GAAGATCTCA GACTCCCCCA
    GGTGTTGCCA CACCACCCAT TCCCAAAATT ACTATTCAGA TTCCTAAGGG AACTGTGATG
    GTGGGCAAGC CCTCTTCTCA CAGTCAGTAT ACCAGCAGTG GTTCTGTGTC TTCCTCAGGA
    AGTAAAAGCC ACCATAGCCA TTCTTCCTTA TCTTCTGCTT CCAACTCAGG CAAGATGAAA
    AGCAGTAAAT CAGAAGGTTC ATCAAGTTCT AAGTTAAGTA GCAGTATATA TTCTAGCCAG
    GGGTCTTCTG GATCTAGCCA ATCCAAAAAT TCATCCCAGT CTGGGGGAAA GCCAGGCTCC
    TCTCCTATTA CCAAGCATGG ACTGAGCAGT GGCTCCAGCA GCACCAAGAT GAAACCTCAA
    GGGAAGCCAT CATCACTTAT GAATCCTTCT TTAAGTAAAC CAAACATATC CCCTTCCCAT
    TCGAGGCCAC CTGGAGGCTC TGATAAGCTT GCCTCTCCAA TGAAGCCTGT TCCTGGGACT
    CCCCCATCCT CTAAAGCCAA GTCCCCTATC AGTTCAGGTT CTGGTGGTTC TCACATGTCT
    GGAACTAGTT CAAGCACCGG CATGAAGTCA TCTTCAGGGT TAGGATCCTC AGGCTCATTG
    TCTCAGAAAA CTCCCCCATC ATCTAACTCT TGTACAGCAT CTTCCTCTTC CTTTTCCTCA
    AGTGGCTCTT CCATGTCATC CTCTCAGAAC CAGCATGGGA GTTCCAAAGG GAAATCTCCC
    AGCAGAAATA AGAAGCCGTC TTTAACAGCT GTCATAGATA AACTGAAGCA TGGGGTTGTC
    ACCAGTGGCC CTGGGGGTGA AGACCCAATG GACGGCCAAA TGGGGGTGAG CACAAATTCT
    AGCCATCCTG TGTCCTCCAA ACATAACATG TCTGGAGGAG AGTTCCAGGG CAAGCGTGAG
    AAAAGTGATA AAGACAAATC AAAGGTTTCC ACCTCGGGGG GCTCAGTGGA TTCATCTAAG
    AAGACCTCAG AGTCTAAAAA TGTGGGGAGC ACGGGTGTGG CAAAAATTAT CATCAGCAAG
    CATGATGGGG GATCCCCCAG CATTAAAGCC AAAGTGACTT TGCAGAAACC TGGGGAAGGT
    GGTGGAGAAG GGCTCAGGCC TCAGATGGCC TCTTCCAAAA ACTATGGCTC TCCACTCATC
    AGTGGTTCCA CTCCAAAGCA TGAGCGTGGC TCTCCCAGCC ATAGTAAGTC ACCAGCATAT
    ACCCCCCAGA ATCTGGACAG TGAAAGTGAG TCAGGCTCCT CCATAGCAGA GAAATCTTAT
    CAGAACAGTC CTAGCTCAGA TGATGGCATC CGACCTCTTC CAGAATACAG CACAGAGAAG
    CATAAGAAGC ACAAAAAAGA AAAGAAGAAA GTAAAGGACA AAGATAGGGA CCGGGACAAA
    GACCGAGACA AGAAAAAATC TCATAGCATC AAGCCAGAGA GTTGGTCTAA ATCACCCATC
    TCTTCAGACC AGTCCTTGTC TATGACAAGT AACACAATCT TATCTACAGA CAGGCCCTCA
    AGGCTCAGCC CTGACTTTAT GATTGGGGAG GAAGATGACG ATCTTATGGA TGTGGCCCTG
    ATTGGCAACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50301
    Clone ID Related Accession (Same CDS sequence) XM_010816249.2 , XM_024980169.1
    Accession Version XM_024980169.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4212bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTACTTGG CTCTCCAATC CTTAGAACAG GATCTATCTA AAATGGCAGT TATGTATTGG 
    AAAGCAACCA ATGCTGGTCC CTTGGACAAG ATTCTTCATG GAAGTGTTGG CTATCTCACT
    CCAAGAAGTG GGGGTCATTT AATGAACTTG AAGTATTATG CCTCTCCTTC TGACCTGCTG
    GATGATAAGA CAACATCCCC TATCATTTTG CATGAGAATA ATGTTCCTCG ATCTTTGGGC
    ATGAATGCAT CAGTGACAAT CGAAGGAACA TCTGCTATGT ATAAACTCCC AATTGCACCA
    TTAATTATGG GATCACATCC AGTTGACAAC AAATGGACTC CCTCCTTCTC CTTAGTTACC
    AGTGCCAACA GTGTTGATCT TCCTGCTTGT TTCTTCTTGA AATTTCCCCA GCCACTTCCA
    GTATCTAGAG CATTTGTTCA GAAAATTCAG AACTGCACAG GAATTCCATT GTTTGAAACT
    CAGCCAACTT ATGTACCCCT CTATGAACTG ATCACTCAAT TTGAACTGTC AAAGGATCCT
    GACCCCTTAC CTTTGAATCA CAACATGCGA TTTTATGCTG CTCTTCCAGG TCAACAGCAC
    TGCTATTTCC TCAACAAGGA TGCTCCTCTT CCAGATGGTA GAAGTCTACA GGGAACCCTT
    GTTAGCAAAA TCACCTTTCA GCACCCTGGC CGGGTTCCTC TTATCCTGAA TCTGATCAGA
    CACCAAGTGG CCTATAACAC CCTAATAGGA AGCTGTGTCA AAAGAACTAT TCTGAAGGAA
    GATTCTCCAG GGCTTCTCCA ATTTGAAGTG TGTCCCCTCT CAGAGTCTCG TTTCAGCGTG
    TCTTTTCAGC ACCCTGTGAA TGACTCCCTG GTGTGTGTGG TAATGGATGT GCAGGACTCA
    ACACATGTGA GCTGTAAACT CTACAAGGGG CTGTCAGATG CACTCATCTG CACAGATGAC
    TTCATTGCCA AAGTTGTTCA AAGATGTATG TCGATTCCTG TGACGATGAG GGCTATTCGG
    AGGAAAGCTG AAACCATTCA GGCCGACACT CCAGCACTGT CCCTCATTGC AGAGACAGTT
    GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA
    GGCAACAACC CAATGAGTGG TACCACTACA CCAACCAACA CCTTTCCGGG GGGTCCCATT
    ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG
    GAGGACTTCA GCAAGGTATC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG
    AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC
    TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAT
    CCTGCCCAGG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCCTCT
    TCCGGCTCAC CCCGGATGGA AATGTGCTCG GGAAGCAACA AGACAAAGAA GAAGAAGTCA
    TCAAGATTAC CACCTGACAA ACCAAAGCAC CAGACTGAAG ATGACTTTCA GAGGGAGCTA
    TTTTCAATGG ATGTTGACTC ACAAAACCCT ATCTTTGATG TCAACATGAC AGCTGACACG
    CTGGATACGC CACACATCAC TCCAGCTCCA AGCCAGTGTA GTACTCCCCC AACAACTTAC
    CCGCAACCAG TACCTCACCC CCAACCCAGT ATTCAAAGGA TGGTCCGACT ATCCAGTTCA
    GACAGCATTG GCCCAGATGT AACTGATATC CTTTCAGACA TTGCAGAAGA AGCTTCTAAG
    CTTCCCAGCA CTAGTGACGA TTGTCCACCC ATTGGCACCC CTGTTCGAGA TTCTTCAAGC
    TCTGGGCATT CTCAGAGTGC CCTCTTTGAC CCTGATGTCT TTCAAGCCAA TAATAATGAA
    AATCCATACA CTGATCCAGC TGACCTTATT GCAGATGCTG CTGGCAGCCC CAGTAGTGAC
    TCACCTACCA ATCATTTTTT TCCTGATGGA GTAGATTTCA ATCCTGATTT GTTGAACAGC
    CAGAGCCAAA GTGGTTTTGG AGAAGAATAT TTTGATGAAA GCAGCCAAAG TGGAGATAAT
    GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGGTGCC AATGCTTGGA
    GGTGATAATG GGGAGACTAA GTTTAAAGGC AATAGCCAAG CTGACACGGT TGATTTCAGT
    ATTATAGCAG TGGCTGGTAA GGCTTTGGGT CCTACAGATC TTATGGAGCA TCACAGTGGT
    AGCCAGAGTC CTTTATTGAC CACTGGGGAC TTAGGGAAAG AAAAGACTCA AAAGAGAGTA
    AAGGAAGGCA ATGGTGCCAG TAATAGTAGT CTGTCAGGGA CAGGATTAGA CAGCAAACCA
    GGGAAGCGCA GTCGAACCCC TTCTAATGAT GGCAAAAGCA AAGATAAGCC TCCAAAGCGG
    AAGAAGGCAG ACACTGAGGG AAAGTCTCCA TCTCACAGTT CTAACCGACC TTTCACCCCA
    CCTACTAGTA CAGGTGGATC CAAATCTCCA GGCAGTTCAG GAAGATCTCA GACTCCCCCA
    GGTGTTGCCA CACCACCCAT TCCCAAAATT ACTATTCAGA TTCCTAAGGG AACTGTGATG
    GTGGGCAAGC CCTCTTCTCA CAGTCAGTAT ACCAGCAGTG GTTCTGTGTC TTCCTCAGGA
    AGTAAAAGCC ACCATAGCCA TTCTTCCTTA TCTTCTGCTT CCAACTCAGG CAAGATGAAA
    AGCAGTAAAT CAGAAGGTTC ATCAAGTTCT AAGTTAAGTA GCAGTATATA TTCTAGCCAG
    GGGTCTTCTG GATCTAGCCA ATCCAAAAAT TCATCCCAGT CTGGGGGAAA GCCAGGCTCC
    TCTCCTATTA CCAAGCATGG ACTGAGCAGT GGCTCCAGCA GCACCAAGAT GAAACCTCAA
    GGGAAGCCAT CATCACTTAT GAATCCTTCT TTAAGTAAAC CAAACATATC CCCTTCCCAT
    TCGAGGCCAC CTGGAGGCTC TGATAAGCTT GCCTCTCCAA TGAAGCCTGT TCCTGGGACT
    CCCCCATCCT CTAAAGCCAA GTCCCCTATC AGTTCAGGTT CTGGTGGTTC TCACATGTCT
    GGAACTAGTT CAAGCACCGG CATGAAGTCA TCTTCAGGGT TAGGATCCTC AGGCTCATTG
    TCTCAGAAAA CTCCCCCATC ATCTAACTCT TGTACAGCAT CTTCCTCTTC CTTTTCCTCA
    AGTGGCTCTT CCATGTCATC CTCTCAGAAC CAGCATGGGA GTTCCAAAGG GAAATCTCCC
    AGCAGAAATA AGAAGCCGTC TTTAACAGCT GTCATAGATA AACTGAAGCA TGGGGTTGTC
    ACCAGTGGCC CTGGGGGTGA AGACCCAATG GACGGCCAAA TGGGGGTGAG CACAAATTCT
    AGCCATCCTG TGTCCTCCAA ACATAACATG TCTGGAGGAG AGTTCCAGGG CAAGCGTGAG
    AAAAGTGATA AAGACAAATC AAAGGTTTCC ACCTCGGGGG GCTCAGTGGA TTCATCTAAG
    AAGACCTCAG AGTCTAAAAA TGTGGGGAGC ACGGGTGTGG CAAAAATTAT CATCAGCAAG
    CATGATGGGG GATCCCCCAG CATTAAAGCC AAAGTGACTT TGCAGAAACC TGGGGAAGGT
    GGTGGAGAAG GGCTCAGGCC TCAGATGGCC TCTTCCAAAA ACTATGGCTC TCCACTCATC
    AGTGGTTCCA CTCCAAAGCA TGAGCGTGGC TCTCCCAGCC ATAGTAAGTC ACCAGCATAT
    ACCCCCCAGA ATCTGGACAG TGAAAGTGAG TCAGGCTCCT CCATAGCAGA GAAATCTTAT
    CAGAACAGTC CTAGCTCAGA TGATGGCATC CGACCTCTTC CAGAATACAG CACAGAGAAG
    CATAAGAAGC ACAAAAAAGA AAAGAAGAAA GTAAAGGACA AAGATAGGGA CCGGGACAAA
    GACCGAGACA AGAAAAAATC TCATAGCATC AAGCCAGAGA GTTGGTCTAA ATCACCCATC
    TCTTCAGACC AGTCCTTGTC TATGACAAGT AACACAATCT TATCTACAGA CAGGCCCTCA
    AGGCTCAGCC CTGACTTTAT GATTGGGGAG GAAGATGACG ATCTTATGGA TGTGGCCCTG
    ATTGGCAACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835937.1
    CDS598..4809
    Translation

    Target ORF information:

    RefSeq Version XM_024980169.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 1 (MED1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980169.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTACTTGG CTCTCCAATC CTTAGAACAG GATCTATCTA AAATGGCAGT TATGTATTGG 
    AAAGCAACCA ATGCTGGTCC CTTGGACAAG ATTCTTCATG GAAGTGTTGG CTATCTCACT
    CCAAGAAGTG GGGGTCATTT AATGAACTTG AAGTATTATG CCTCTCCTTC TGACCTGCTG
    GATGATAAGA CAACATCCCC TATCATTTTG CATGAGAATA ATGTTCCTCG ATCTTTGGGC
    ATGAATGCAT CAGTGACAAT CGAAGGAACA TCTGCTATGT ATAAACTCCC AATTGCACCA
    TTAATTATGG GATCACATCC AGTTGACAAC AAATGGACTC CCTCCTTCTC CTTAGTTACC
    AGTGCCAACA GTGTTGATCT TCCTGCTTGT TTCTTCTTGA AATTTCCCCA GCCACTTCCA
    GTATCTAGAG CATTTGTTCA GAAAATTCAG AACTGCACAG GAATTCCATT GTTTGAAACT
    CAGCCAACTT ATGTACCCCT CTATGAACTG ATCACTCAAT TTGAACTGTC AAAGGATCCT
    GACCCCTTAC CTTTGAATCA CAACATGCGA TTTTATGCTG CTCTTCCAGG TCAACAGCAC
    TGCTATTTCC TCAACAAGGA TGCTCCTCTT CCAGATGGTA GAAGTCTACA GGGAACCCTT
    GTTAGCAAAA TCACCTTTCA GCACCCTGGC CGGGTTCCTC TTATCCTGAA TCTGATCAGA
    CACCAAGTGG CCTATAACAC CCTAATAGGA AGCTGTGTCA AAAGAACTAT TCTGAAGGAA
    GATTCTCCAG GGCTTCTCCA ATTTGAAGTG TGTCCCCTCT CAGAGTCTCG TTTCAGCGTG
    TCTTTTCAGC ACCCTGTGAA TGACTCCCTG GTGTGTGTGG TAATGGATGT GCAGGACTCA
    ACACATGTGA GCTGTAAACT CTACAAGGGG CTGTCAGATG CACTCATCTG CACAGATGAC
    TTCATTGCCA AAGTTGTTCA AAGATGTATG TCGATTCCTG TGACGATGAG GGCTATTCGG
    AGGAAAGCTG AAACCATTCA GGCCGACACT CCAGCACTGT CCCTCATTGC AGAGACAGTT
    GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA
    GGCAACAACC CAATGAGTGG TACCACTACA CCAACCAACA CCTTTCCGGG GGGTCCCATT
    ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG
    GAGGACTTCA GCAAGGTATC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG
    AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC
    TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAT
    CCTGCCCAGG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCCTCT
    TCCGGCTCAC CCCGGATGGA AATGTGCTCG GGAAGCAACA AGACAAAGAA GAAGAAGTCA
    TCAAGATTAC CACCTGACAA ACCAAAGCAC CAGACTGAAG ATGACTTTCA GAGGGAGCTA
    TTTTCAATGG ATGTTGACTC ACAAAACCCT ATCTTTGATG TCAACATGAC AGCTGACACG
    CTGGATACGC CACACATCAC TCCAGCTCCA AGCCAGTGTA GTACTCCCCC AACAACTTAC
    CCGCAACCAG TACCTCACCC CCAACCCAGT ATTCAAAGGA TGGTCCGACT ATCCAGTTCA
    GACAGCATTG GCCCAGATGT AACTGATATC CTTTCAGACA TTGCAGAAGA AGCTTCTAAG
    CTTCCCAGCA CTAGTGACGA TTGTCCACCC ATTGGCACCC CTGTTCGAGA TTCTTCAAGC
    TCTGGGCATT CTCAGAGTGC CCTCTTTGAC CCTGATGTCT TTCAAGCCAA TAATAATGAA
    AATCCATACA CTGATCCAGC TGACCTTATT GCAGATGCTG CTGGCAGCCC CAGTAGTGAC
    TCACCTACCA ATCATTTTTT TCCTGATGGA GTAGATTTCA ATCCTGATTT GTTGAACAGC
    CAGAGCCAAA GTGGTTTTGG AGAAGAATAT TTTGATGAAA GCAGCCAAAG TGGAGATAAT
    GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGGTGCC AATGCTTGGA
    GGTGATAATG GGGAGACTAA GTTTAAAGGC AATAGCCAAG CTGACACGGT TGATTTCAGT
    ATTATAGCAG TGGCTGGTAA GGCTTTGGGT CCTACAGATC TTATGGAGCA TCACAGTGGT
    AGCCAGAGTC CTTTATTGAC CACTGGGGAC TTAGGGAAAG AAAAGACTCA AAAGAGAGTA
    AAGGAAGGCA ATGGTGCCAG TAATAGTAGT CTGTCAGGGA CAGGATTAGA CAGCAAACCA
    GGGAAGCGCA GTCGAACCCC TTCTAATGAT GGCAAAAGCA AAGATAAGCC TCCAAAGCGG
    AAGAAGGCAG ACACTGAGGG AAAGTCTCCA TCTCACAGTT CTAACCGACC TTTCACCCCA
    CCTACTAGTA CAGGTGGATC CAAATCTCCA GGCAGTTCAG GAAGATCTCA GACTCCCCCA
    GGTGTTGCCA CACCACCCAT TCCCAAAATT ACTATTCAGA TTCCTAAGGG AACTGTGATG
    GTGGGCAAGC CCTCTTCTCA CAGTCAGTAT ACCAGCAGTG GTTCTGTGTC TTCCTCAGGA
    AGTAAAAGCC ACCATAGCCA TTCTTCCTTA TCTTCTGCTT CCAACTCAGG CAAGATGAAA
    AGCAGTAAAT CAGAAGGTTC ATCAAGTTCT AAGTTAAGTA GCAGTATATA TTCTAGCCAG
    GGGTCTTCTG GATCTAGCCA ATCCAAAAAT TCATCCCAGT CTGGGGGAAA GCCAGGCTCC
    TCTCCTATTA CCAAGCATGG ACTGAGCAGT GGCTCCAGCA GCACCAAGAT GAAACCTCAA
    GGGAAGCCAT CATCACTTAT GAATCCTTCT TTAAGTAAAC CAAACATATC CCCTTCCCAT
    TCGAGGCCAC CTGGAGGCTC TGATAAGCTT GCCTCTCCAA TGAAGCCTGT TCCTGGGACT
    CCCCCATCCT CTAAAGCCAA GTCCCCTATC AGTTCAGGTT CTGGTGGTTC TCACATGTCT
    GGAACTAGTT CAAGCACCGG CATGAAGTCA TCTTCAGGGT TAGGATCCTC AGGCTCATTG
    TCTCAGAAAA CTCCCCCATC ATCTAACTCT TGTACAGCAT CTTCCTCTTC CTTTTCCTCA
    AGTGGCTCTT CCATGTCATC CTCTCAGAAC CAGCATGGGA GTTCCAAAGG GAAATCTCCC
    AGCAGAAATA AGAAGCCGTC TTTAACAGCT GTCATAGATA AACTGAAGCA TGGGGTTGTC
    ACCAGTGGCC CTGGGGGTGA AGACCCAATG GACGGCCAAA TGGGGGTGAG CACAAATTCT
    AGCCATCCTG TGTCCTCCAA ACATAACATG TCTGGAGGAG AGTTCCAGGG CAAGCGTGAG
    AAAAGTGATA AAGACAAATC AAAGGTTTCC ACCTCGGGGG GCTCAGTGGA TTCATCTAAG
    AAGACCTCAG AGTCTAAAAA TGTGGGGAGC ACGGGTGTGG CAAAAATTAT CATCAGCAAG
    CATGATGGGG GATCCCCCAG CATTAAAGCC AAAGTGACTT TGCAGAAACC TGGGGAAGGT
    GGTGGAGAAG GGCTCAGGCC TCAGATGGCC TCTTCCAAAA ACTATGGCTC TCCACTCATC
    AGTGGTTCCA CTCCAAAGCA TGAGCGTGGC TCTCCCAGCC ATAGTAAGTC ACCAGCATAT
    ACCCCCCAGA ATCTGGACAG TGAAAGTGAG TCAGGCTCCT CCATAGCAGA GAAATCTTAT
    CAGAACAGTC CTAGCTCAGA TGATGGCATC CGACCTCTTC CAGAATACAG CACAGAGAAG
    CATAAGAAGC ACAAAAAAGA AAAGAAGAAA GTAAAGGACA AAGATAGGGA CCGGGACAAA
    GACCGAGACA AGAAAAAATC TCATAGCATC AAGCCAGAGA GTTGGTCTAA ATCACCCATC
    TCTTCAGACC AGTCCTTGTC TATGACAAGT AACACAATCT TATCTACAGA CAGGCCCTCA
    AGGCTCAGCC CTGACTTTAT GATTGGGGAG GAAGATGACG ATCTTATGGA TGTGGCCCTG
    ATTGGCAACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.