Dgke cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dgke
Entrez Gene ID	497978
Full Name	diacylglycerol kinase epsilon
General protein information	Preferred Names diacylglycerol kinase epsilon Names diacylglycerol kinase epsilon diacylglycerol kinase, epsilon 64kDa
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q26

mRNA and Protein(s)

mRNA	Protein	Name
XM_006247070.3	XP_006247132.1	diacylglycerol kinase epsilon isoform X1
XM_017597469.1	XP_017452958.1	diacylglycerol kinase epsilon isoform X3
NM_001039341.1	NP_001034430.1	diacylglycerol kinase epsilon
XM_006247071.3	XP_006247133.1	diacylglycerol kinase epsilon isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Dgke	NP_001034430.1
	DGKE	XP_001101454.2

Gene Ontology
Bibliography

Related articles in PubMed

Diacylglycerol kinase ? localizes to subsurface cisterns of cerebellar Purkinje cells.
Hozumi Y, Fujiwara H, Kaneko K, Fujii S, Topham MK, Watanabe M, Goto K
Cell and tissue research368(3)441-458(2017 06)

Distinct expression and localization of diacylglycerol kinase isozymes in rat retina.
Hozumi Y, Matsui H, Sakane F, Watanabe M, Goto K
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society61(6)462-76(2013 Jun)

Recessive mutations in DGKE cause atypical hemolytic-uremic syndrome.
Lemaire M, Fr?meaux-Bacchi V, Schaefer F, Choi M, Tang WH, Le Quintrec M, Fakhouri F, Taque S, Nobili F, Martinez F, Ji W, Overton JD, Mane SM, N?rnberg G, Altm?ller J, Thiele H, Morin D, Deschenes G, Baudouin V, Llanas B, Collard L, Majid MA, Simkova E, N?rnberg P, Rioux-Leclerc N, Moeckel GW, Gubler MC, Hwa J, Loirat C, Lifton RP
Nature genetics45(5)531-6(2013 May)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Localization of diacylglycerol kinase epsilon on stress fibers in vascular smooth muscle cells.
Nakano T, Hozumi Y, Goto K, Wakabayashi I
Cell and tissue research337(1)167-75(2009 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The DGKepsilon is evident in the outer limiting membrane.
Title: Distinct expression and localization of diacylglycerol kinase isozymes in rat retina.

DGKepsilon interacts with actin stress fibers and is involved in their stability in vascular smooth muscle cells.
Title: Localization of diacylglycerol kinase epsilon on stress fibers in vascular smooth muscle cells.

Diacylglycerol kinase-epsilon restores cardiac dysfunction under chronic pressure overload: a new specific regulator of Galpha(q) signaling cascade.
Title: Diacylglycerol kinase-epsilon restores cardiac dysfunction under chronic pressure overload: a new specific regulator of Galpha(q) signaling cascade.

The following Dgke gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dgke cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa09728	NM_001039341.1 Latest version!	Rattus norvegicus diacylglycerol kinase epsilon (Dgke), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa33549	XM_006247070.3 Latest version!	Rattus norvegicus diacylglycerol kinase epsilon (Dgke), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa33550	XM_006247071.3 Latest version!	Rattus norvegicus diacylglycerol kinase epsilon (Dgke), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa57439	XM_017597469.1 Latest version!	Rattus norvegicus diacylglycerol kinase epsilon (Dgke), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dgke ( NM_001039341.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001034430.1 Rattus norvegicus diacylglycerol kinase epsilon (Dgke), mRNA.

Price & Availability↑

CloneID	ORa09728
Clone ID Related Accession (Same CDS sequence)	NM_001039341.1
Accession Version	NM_001039341.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase epsilon
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC110049.1. On Jan 31, 2006 this sequence version replaced XM_573174.1. ##Evidence-Data-START## Transcript exon combination :: BC110049.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGGAG ACCAGCGGTC CGGCCCATCG GCGCAGGGCC TGCTCCCCGA TGGCCACTTG 
ATCCTATGGA CGCTGTTCTC CGTGCTGTTG CCGGTGTTCA TCACCTTATG GTGTAGTCTG 
CAGCGTTCGC GCCGGCAGCT GCACCGCAGG GACATCTTCC GCAAGAGCAA GCACTGCTGG 
CGTGACACCG ACCTGTTCAG CCACCCCACC TACTGCTGCG TGTGCGCCCA GCACATCCTG 
CAAGGAGCCT TCTGCGACTG CTGCGGGCTC CGCGTGGACG AGGGCTGCCT CAAGAAGGTC 
GACAAGCGCT TCCCGTGCAA GGAGATCATG CTCAAGAACG ACAGCAGGGC CGCAGATGCG 
ATGCCCCACC ACTGGATCCG CGGCAACGTC CCCCTGTGCA CTTACTGTGT AATCTGCAGG 
CAGCAGTGTG GCAGCCAGCC TAAACTCTGC GACTACCGGT GTGTTTGGTG CCAGCAGACA 
GTCCACGATG AGTGCATGAG AGGCAGCTTA AAGAGTGAGA AGTGTGATTT TGGAGAATTC 
AAAAATCTCA TCATCCCTCC AAGTTATTTA ACGTCCATTA ACCAGATGCG TAAAGACAAA 
AACACAAATT ATGAAGTGCT TGCTTCTAAG TTTGGAAAGC AGTGGACCCC ATTAATAATC 
CTGGCCAACT CTCGTAGTGG AACTAACATG GGAGAAGGAC TGTTGGGAGA GTTTAAGATG 
CTGTTAAATC CCGTCCAGGT GTTCGATGTC ACTAAAACTC CTCCGATCAA AGCTCTGCAG 
CTGTGCACTC TTCTTCCTTA TTACTCCGTT CGAGTCCTTG TTTGTGGAGG GGATGGGACT 
GTGGGCTGGG TCCTCGATGC AATTGATGAA ATGAAGATTA AGGGACAAGA AAAATACATT 
CCAGAGGTTG CAGTCTTACC TCTGGGAACA GGCAACGACC TATCCAACAC CCTGGGCTGG 
GGTACTGGCT ATGCTGGAGA AATCCCGGTT GCACAGGTCT TAAGGAATGT AATGGAAGCA 
GATGGAATCA AACTAGACAG ATGGAAAGTG CAGGTAACAA ATAAAGGCTA CTACAGTTTA 
AGGAAACCCA AGGAGTTCAC AATGAACAAC TACTTCTCTA TTGGACCCGA TGCCCTCATG 
GCTCTCAATT TTCATGCTCA TCGTGAGAAG GCGCCGTCTC TGTTTTCCAG CCGAATCCTT 
AACAAGGTGT GTTGGATAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034430.1
CDS	170..1393
Translation	MEGDQRSGPSAQGLLPDGHLILWTLFSVLLPVFITLWCSLQRSRRQLHRRDIFRKSKHCWRDTDLFSHPTYCCVCAQHILQGAFCDCCGLRVDEGCLKKVDKRFPCKEIMLKNDSRAADAMPHHWIRGNVPLCTYCVICRQQCGSQPKLCDYRCVWCQQTVHDECMRGSLKSEKCDFGEFKNLIIPPSYLTSINQMRKDKNTNYEVLASKFGKQWTPLIILANSRSGTNMGEGLLGEFKMLLNPVQVFDVTKTPPIKALQLCTLLPYYSVRVLVCGGDGTVGWVLDAIDEMKIKGQEKYIPEVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGIKLDRWKVQVTNKGYYSLRKPKEFTMNNYFSIGPDALMALNFHAHREKAPSLFSSRILNKVCWIK

Target ORF information:

RefSeq Version	NM_001039341.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase epsilon (Dgke), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039341.1

ORF Insert Sequence:

ATGGAGGGAG ACCAGCGGTC CGGCCCATCG GCGCAGGGCC TGCTCCCCGA TGGCCACTTG 
ATCCTATGGA CGCTGTTCTC CGTGCTGTTG CCGGTGTTCA TCACCTTATG GTGTAGTCTG 
CAGCGTTCGC GCCGGCAGCT GCACCGCAGG GACATCTTCC GCAAGAGCAA GCACTGCTGG 
CGTGACACCG ACCTGTTCAG CCACCCCACC TACTGCTGCG TGTGCGCCCA GCACATCCTG 
CAAGGAGCCT TCTGCGACTG CTGCGGGCTC CGCGTGGACG AGGGCTGCCT CAAGAAGGTC 
GACAAGCGCT TCCCGTGCAA GGAGATCATG CTCAAGAACG ACAGCAGGGC CGCAGATGCG 
ATGCCCCACC ACTGGATCCG CGGCAACGTC CCCCTGTGCA CTTACTGTGT AATCTGCAGG 
CAGCAGTGTG GCAGCCAGCC TAAACTCTGC GACTACCGGT GTGTTTGGTG CCAGCAGACA 
GTCCACGATG AGTGCATGAG AGGCAGCTTA AAGAGTGAGA AGTGTGATTT TGGAGAATTC 
AAAAATCTCA TCATCCCTCC AAGTTATTTA ACGTCCATTA ACCAGATGCG TAAAGACAAA 
AACACAAATT ATGAAGTGCT TGCTTCTAAG TTTGGAAAGC AGTGGACCCC ATTAATAATC 
CTGGCCAACT CTCGTAGTGG AACTAACATG GGAGAAGGAC TGTTGGGAGA GTTTAAGATG 
CTGTTAAATC CCGTCCAGGT GTTCGATGTC ACTAAAACTC CTCCGATCAA AGCTCTGCAG 
CTGTGCACTC TTCTTCCTTA TTACTCCGTT CGAGTCCTTG TTTGTGGAGG GGATGGGACT 
GTGGGCTGGG TCCTCGATGC AATTGATGAA ATGAAGATTA AGGGACAAGA AAAATACATT 
CCAGAGGTTG CAGTCTTACC TCTGGGAACA GGCAACGACC TATCCAACAC CCTGGGCTGG 
GGTACTGGCT ATGCTGGAGA AATCCCGGTT GCACAGGTCT TAAGGAATGT AATGGAAGCA 
GATGGAATCA AACTAGACAG ATGGAAAGTG CAGGTAACAA ATAAAGGCTA CTACAGTTTA 
AGGAAACCCA AGGAGTTCAC AATGAACAAC TACTTCTCTA TTGGACCCGA TGCCCTCATG 
GCTCTCAATT TTCATGCTCA TCGTGAGAAG GCGCCGTCTC TGTTTTCCAG CCGAATCCTT 
AACAAGGTGT GTTGGATAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgke ( XM_006247070.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247132.1 Rattus norvegicus diacylglycerol kinase epsilon (Dgke), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa33549
Clone ID Related Accession (Same CDS sequence)	XM_006247070.3
Accession Version	XM_006247070.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase epsilon isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247070.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAG ACCAGCGGTC CGGCCCATCG GCGCAGGGCC TGCTCCCCGA TGGCCACTTG 
ATCCTATGGA CGCTGTTCTC CGTGCTGTTG CCGGTGTTCA TCACCTTATG GTGTAGTCTG 
CAGCGTTCGC GCCGGCAGCT GCACCGCAGG GACATCTTCC GCAAGAGCAA GCACTGCTGG 
CGTGACACCG ACCTGTTCAG CCACCCCACC TACTGCTGCG TGTGCGCCCA GCACATCCTG 
CAAGGAGCCT TCTGCGACTG CTGCGGGCTC CGCGTGGACG AGGGCTGCCT CAAGAAGGTC 
GACAAGCGCT TCCCGTGCAA GGAGATCATG CTCAAGAACG ACAGCAGGGC CGCAGATGCG 
ATGCCCCACC ACTGGATCCG CGGCAACGTC CCCCTGTGCA CTTACTGTGT AATCTGCAGG 
CAGCAGTGTG GCAGCCAGCC TAAACTCTGC GACTACCGGT GTGTTTGGTG CCAGCAGACA 
GTCCACGATG AGTGCATGAG AGGCAGCTTA AAGAGTGAGA AGTGTGATTT TGGAGAATTC 
AAAAATCTCA TCATCCCTCC AAGTTATTTA ACGTCCATTA ACCAGATGCG TAAAGACAAA 
AACACAAATT ATGAAGTGCT TGCTTCTAAG TTTGGAAAGC AGTGGACCCC ATTAATAATC 
CTGGCCAACT CTCGTAGTGG AACTAACATG GGAGAAGGAC TGTTGGGAGA GTTTAAGATG 
CTGTTAAATC CCGTCCAGGT GTTCGATGTC ACTAAAACTC CTCCGATCAA AGCTCTGCAG 
CTGTGCACTC TTCTTCCTTA TTACTCCGTT CGAGTCCTTG TTTGTGGAGG GGATGGGACT 
GTGGGCTGGG TCCTCGATGC AATTGATGAA ATGAAGATTA AGGGACAAGA AAAATACATT 
CCAGAGGTTG CAGTCTTACC TCTGGGAACA GGCAACGACC TATCCAACAC CCTGGGCTGG 
GGTACTGGCT ATGCTGGAGA AATCCCGGTT GCACAGGTCT TAAGGAATGT AATGGAAGCA 
GATGGAATCA AACTAGACAG ATGGAAAGTG CAGGTAACAA ATAAAGGCTA CTACAGTTTA 
AGGAAACCCA AGGAGTTCAC AATGAACAAC TACTTCTCTA TTGGACCCGA TGCCCTCATG 
GCTCTCAATT TTCATGCTCA TCGTGAGAAG GCGCCGTCTC TGTTTTCCAG CCGAATCCTT 
AACAAGGCTG TTTATTTATT CTATGGAACT AGAGATTGCT TGGTGCAAGA GTGTAAAGAT 
TTGAATAAAA AAATTGAGCT AGAGCTGGAT GGTGAGCGAG TTGAACTGCC TAACTTGGAG 
GGAGTTATTG TTCTGAACAT TGGCTACTGG GGCGGTGGCT GCCGACTGTG GGAAGGAGTG 
GGAGATGAGA CGTATCCTCT AGCCAGACAT GATGATGGCT TACTGGAAGT TGTTGGAGTA 
TATGGGTCTT TCCACTGTGC TCAAATCCAG GTGAAACTGG CAAATCCTTT TCGAATCGGA 
CAGGCCCATA CCGTGCGGCT GACTTTGAAG TGCTCCAGGA TGCCGATGCC GATGCAGGTG 
GATGGGGAGC CCTGGGCCCA AGGGCCCTGT ACTGTCACCA TAACGCACAA GACACATGCA 
CTGATGCTGT ACTTCTCTGG GGAGCAGTCG GATGATGACC TCTCAAGCCC TTCAGATCAC 
GAGGACTTAA AGGAGGCCGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247132.1
CDS	399..2102
Translation	MEGDQRSGPSAQGLLPDGHLILWTLFSVLLPVFITLWCSLQRSRRQLHRRDIFRKSKHCWRDTDLFSHPTYCCVCAQHILQGAFCDCCGLRVDEGCLKKVDKRFPCKEIMLKNDSRAADAMPHHWIRGNVPLCTYCVICRQQCGSQPKLCDYRCVWCQQTVHDECMRGSLKSEKCDFGEFKNLIIPPSYLTSINQMRKDKNTNYEVLASKFGKQWTPLIILANSRSGTNMGEGLLGEFKMLLNPVQVFDVTKTPPIKALQLCTLLPYYSVRVLVCGGDGTVGWVLDAIDEMKIKGQEKYIPEVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGIKLDRWKVQVTNKGYYSLRKPKEFTMNNYFSIGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTRDCLVQECKDLNKKIELELDGERVELPNLEGVIVLNIGYWGGGCRLWEGVGDETYPLARHDDGLLEVVGVYGSFHCAQIQVKLANPFRIGQAHTVRLTLKCSRMPMPMQVDGEPWAQGPCTVTITHKTHALMLYFSGEQSDDDLSSPSDHEDLKEAE

Target ORF information:

RefSeq Version	XM_006247070.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase epsilon (Dgke), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247070.3

ORF Insert Sequence:

ATGGAGGGAG ACCAGCGGTC CGGCCCATCG GCGCAGGGCC TGCTCCCCGA TGGCCACTTG 
ATCCTATGGA CGCTGTTCTC CGTGCTGTTG CCGGTGTTCA TCACCTTATG GTGTAGTCTG 
CAGCGTTCGC GCCGGCAGCT GCACCGCAGG GACATCTTCC GCAAGAGCAA GCACTGCTGG 
CGTGACACCG ACCTGTTCAG CCACCCCACC TACTGCTGCG TGTGCGCCCA GCACATCCTG 
CAAGGAGCCT TCTGCGACTG CTGCGGGCTC CGCGTGGACG AGGGCTGCCT CAAGAAGGTC 
GACAAGCGCT TCCCGTGCAA GGAGATCATG CTCAAGAACG ACAGCAGGGC CGCAGATGCG 
ATGCCCCACC ACTGGATCCG CGGCAACGTC CCCCTGTGCA CTTACTGTGT AATCTGCAGG 
CAGCAGTGTG GCAGCCAGCC TAAACTCTGC GACTACCGGT GTGTTTGGTG CCAGCAGACA 
GTCCACGATG AGTGCATGAG AGGCAGCTTA AAGAGTGAGA AGTGTGATTT TGGAGAATTC 
AAAAATCTCA TCATCCCTCC AAGTTATTTA ACGTCCATTA ACCAGATGCG TAAAGACAAA 
AACACAAATT ATGAAGTGCT TGCTTCTAAG TTTGGAAAGC AGTGGACCCC ATTAATAATC 
CTGGCCAACT CTCGTAGTGG AACTAACATG GGAGAAGGAC TGTTGGGAGA GTTTAAGATG 
CTGTTAAATC CCGTCCAGGT GTTCGATGTC ACTAAAACTC CTCCGATCAA AGCTCTGCAG 
CTGTGCACTC TTCTTCCTTA TTACTCCGTT CGAGTCCTTG TTTGTGGAGG GGATGGGACT 
GTGGGCTGGG TCCTCGATGC AATTGATGAA ATGAAGATTA AGGGACAAGA AAAATACATT 
CCAGAGGTTG CAGTCTTACC TCTGGGAACA GGCAACGACC TATCCAACAC CCTGGGCTGG 
GGTACTGGCT ATGCTGGAGA AATCCCGGTT GCACAGGTCT TAAGGAATGT AATGGAAGCA 
GATGGAATCA AACTAGACAG ATGGAAAGTG CAGGTAACAA ATAAAGGCTA CTACAGTTTA 
AGGAAACCCA AGGAGTTCAC AATGAACAAC TACTTCTCTA TTGGACCCGA TGCCCTCATG 
GCTCTCAATT TTCATGCTCA TCGTGAGAAG GCGCCGTCTC TGTTTTCCAG CCGAATCCTT 
AACAAGGCTG TTTATTTATT CTATGGAACT AGAGATTGCT TGGTGCAAGA GTGTAAAGAT 
TTGAATAAAA AAATTGAGCT AGAGCTGGAT GGTGAGCGAG TTGAACTGCC TAACTTGGAG 
GGAGTTATTG TTCTGAACAT TGGCTACTGG GGCGGTGGCT GCCGACTGTG GGAAGGAGTG 
GGAGATGAGA CGTATCCTCT AGCCAGACAT GATGATGGCT TACTGGAAGT TGTTGGAGTA 
TATGGGTCTT TCCACTGTGC TCAAATCCAG GTGAAACTGG CAAATCCTTT TCGAATCGGA 
CAGGCCCATA CCGTGCGGCT GACTTTGAAG TGCTCCAGGA TGCCGATGCC GATGCAGGTG 
GATGGGGAGC CCTGGGCCCA AGGGCCCTGT ACTGTCACCA TAACGCACAA GACACATGCA 
CTGATGCTGT ACTTCTCTGG GGAGCAGTCG GATGATGACC TCTCAAGCCC TTCAGATCAC 
GAGGACTTAA AGGAGGCCGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgke ( XM_006247071.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247133.1 Rattus norvegicus diacylglycerol kinase epsilon (Dgke), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa33550
Clone ID Related Accession (Same CDS sequence)	XM_006247071.3
Accession Version	XM_006247071.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase epsilon isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247071.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAG ACCAGCGGTC CGGCCCATCG GCGCAGGGCC TGCTCCCCGA TGGCCACTTG 
ATCCTATGGA CGCTGTTCTC CGTGCTGTTG CCGGTGTTCA TCACCTTATG GTGTAGTCTG 
CAGCGTTCGC GCCGGCAGCT GCACCGCAGG GACATCTTCC GCAAGAGCAA GCACTGCTGG 
CGTGACACCG ACCTGTTCAG CCACCCCACC TACTGCTGCG TGTGCGCCCA GCACATCCTG 
CAAGGAGCCT TCTGCGACTG CTGCGGGCTC CGCGTGGACG AGGGCTGCCT CAAGAAGGTC 
GACAAGCGCT TCCCGTGCAA GGAGATCATG CTCAAGAACG ACAGCAGGGC CGCAGATGCG 
ATGCCCCACC ACTGGATCCG CGGCAACGTC CCCCTGTGCA CTTACTGTGT AATCTGCAGG 
CAGCAGTGTG GCAGCCAGCC TAAACTCTGC GACTACCGGT GTGTTTGGTG CCAGCAGACA 
GTCCACGATG AGTGCATGAG AGGCAGCTTA AAGAGTGAGA AGTGTGATTT TGGAGAATTC 
AAAAATCTCA TCATCCCTCC AAGTTATTTA ACGTCCATTA ACCAGATGCG TAAAGACAAA 
AACACAAATT ATGAAGTGCT TGCTTCTAAG TTTGGAAAGC AGTGGACCCC ATTAATAATC 
CTGGCCAACT CTCGTAGTGG AACTAACATG GGAGAAGGAC TGTTGGGAGA GTTTAAGATG 
CTGTTAAATC CCGTCCAGGT GTTCGATGTC ACTAAAACTC CTCCGATCAA AGCTCTGCAG 
CTGTGCACTC TTCTTCCTTA TTACTCCGTT CGAGTCCTTG TTTGTGGAGG GGATGGGACT 
GTGGGCTGGG TCCTCGATGC AATTGATGAA ATGAAGATTA AGGGACAAGA AAAATACATT 
CCAGAGGTTG CAGTCTTACC TCTGGGAACA GGCAACGACC TATCCAACAC CCTGGGCTGG 
GGTACTGGCT ATGCTGGAGA AATCCCGGTT GCACAGGTCT TAAGGAATGT AATGGAAGCA 
GATGGAATCA AACTAGACAG ATGGAAAGTG CAGGTAACAA ATAAAGGCTA CTACAGTTTA 
AGGAAACCCA AGGAGTTCAC AATGAACAAC TACTTCTCTA TTGGACCCGA TGCCCTCATG 
GCTCTCAATT TTCATGCTCA TCGTGAGAAG GCGCCGTCTC TGTTTTCCAG CCGAATCCTT 
AACAAGCTAG AGCTGGATGG TGAGCGAGTT GAACTGCCTA ACTTGGAGGG AGTTATTGTT 
CTGAACATTG GCTACTGGGG CGGTGGCTGC CGACTGTGGG AAGGAGTGGG AGATGAGACG 
TATCCTCTAG CCAGACATGA TGATGGCTTA CTGGAAGTTG TTGGAGTATA TGGGTCTTTC 
CACTGTGCTC AAATCCAGGT GAAACTGGCA AATCCTTTTC GAATCGGACA GGCCCATACC 
GTGCGGCTGA CTTTGAAGTG CTCCAGGATG CCGATGCCGA TGCAGGTGGA TGGGGAGCCC 
TGGGCCCAAG GGCCCTGTAC TGTCACCATA ACGCACAAGA CACATGCACT GATGCTGTAC 
TTCTCTGGGG AGCAGTCGGA TGATGACCTC TCAAGCCCTT CAGATCACGA GGACTTAAAG 
GAGGCCGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247133.1
CDS	400..2031
Translation	MEGDQRSGPSAQGLLPDGHLILWTLFSVLLPVFITLWCSLQRSRRQLHRRDIFRKSKHCWRDTDLFSHPTYCCVCAQHILQGAFCDCCGLRVDEGCLKKVDKRFPCKEIMLKNDSRAADAMPHHWIRGNVPLCTYCVICRQQCGSQPKLCDYRCVWCQQTVHDECMRGSLKSEKCDFGEFKNLIIPPSYLTSINQMRKDKNTNYEVLASKFGKQWTPLIILANSRSGTNMGEGLLGEFKMLLNPVQVFDVTKTPPIKALQLCTLLPYYSVRVLVCGGDGTVGWVLDAIDEMKIKGQEKYIPEVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGIKLDRWKVQVTNKGYYSLRKPKEFTMNNYFSIGPDALMALNFHAHREKAPSLFSSRILNKLELDGERVELPNLEGVIVLNIGYWGGGCRLWEGVGDETYPLARHDDGLLEVVGVYGSFHCAQIQVKLANPFRIGQAHTVRLTLKCSRMPMPMQVDGEPWAQGPCTVTITHKTHALMLYFSGEQSDDDLSSPSDHEDLKEAE

Target ORF information:

RefSeq Version	XM_006247071.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase epsilon (Dgke), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247071.3

ORF Insert Sequence:

ATGGAGGGAG ACCAGCGGTC CGGCCCATCG GCGCAGGGCC TGCTCCCCGA TGGCCACTTG 
ATCCTATGGA CGCTGTTCTC CGTGCTGTTG CCGGTGTTCA TCACCTTATG GTGTAGTCTG 
CAGCGTTCGC GCCGGCAGCT GCACCGCAGG GACATCTTCC GCAAGAGCAA GCACTGCTGG 
CGTGACACCG ACCTGTTCAG CCACCCCACC TACTGCTGCG TGTGCGCCCA GCACATCCTG 
CAAGGAGCCT TCTGCGACTG CTGCGGGCTC CGCGTGGACG AGGGCTGCCT CAAGAAGGTC 
GACAAGCGCT TCCCGTGCAA GGAGATCATG CTCAAGAACG ACAGCAGGGC CGCAGATGCG 
ATGCCCCACC ACTGGATCCG CGGCAACGTC CCCCTGTGCA CTTACTGTGT AATCTGCAGG 
CAGCAGTGTG GCAGCCAGCC TAAACTCTGC GACTACCGGT GTGTTTGGTG CCAGCAGACA 
GTCCACGATG AGTGCATGAG AGGCAGCTTA AAGAGTGAGA AGTGTGATTT TGGAGAATTC 
AAAAATCTCA TCATCCCTCC AAGTTATTTA ACGTCCATTA ACCAGATGCG TAAAGACAAA 
AACACAAATT ATGAAGTGCT TGCTTCTAAG TTTGGAAAGC AGTGGACCCC ATTAATAATC 
CTGGCCAACT CTCGTAGTGG AACTAACATG GGAGAAGGAC TGTTGGGAGA GTTTAAGATG 
CTGTTAAATC CCGTCCAGGT GTTCGATGTC ACTAAAACTC CTCCGATCAA AGCTCTGCAG 
CTGTGCACTC TTCTTCCTTA TTACTCCGTT CGAGTCCTTG TTTGTGGAGG GGATGGGACT 
GTGGGCTGGG TCCTCGATGC AATTGATGAA ATGAAGATTA AGGGACAAGA AAAATACATT 
CCAGAGGTTG CAGTCTTACC TCTGGGAACA GGCAACGACC TATCCAACAC CCTGGGCTGG 
GGTACTGGCT ATGCTGGAGA AATCCCGGTT GCACAGGTCT TAAGGAATGT AATGGAAGCA 
GATGGAATCA AACTAGACAG ATGGAAAGTG CAGGTAACAA ATAAAGGCTA CTACAGTTTA 
AGGAAACCCA AGGAGTTCAC AATGAACAAC TACTTCTCTA TTGGACCCGA TGCCCTCATG 
GCTCTCAATT TTCATGCTCA TCGTGAGAAG GCGCCGTCTC TGTTTTCCAG CCGAATCCTT 
AACAAGCTAG AGCTGGATGG TGAGCGAGTT GAACTGCCTA ACTTGGAGGG AGTTATTGTT 
CTGAACATTG GCTACTGGGG CGGTGGCTGC CGACTGTGGG AAGGAGTGGG AGATGAGACG 
TATCCTCTAG CCAGACATGA TGATGGCTTA CTGGAAGTTG TTGGAGTATA TGGGTCTTTC 
CACTGTGCTC AAATCCAGGT GAAACTGGCA AATCCTTTTC GAATCGGACA GGCCCATACC 
GTGCGGCTGA CTTTGAAGTG CTCCAGGATG CCGATGCCGA TGCAGGTGGA TGGGGAGCCC 
TGGGCCCAAG GGCCCTGTAC TGTCACCATA ACGCACAAGA CACATGCACT GATGCTGTAC 
TTCTCTGGGG AGCAGTCGGA TGATGACCTC TCAAGCCCTT CAGATCACGA GGACTTAAAG 
GAGGCCGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgke ( XM_017597469.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452958.1 Rattus norvegicus diacylglycerol kinase epsilon (Dgke), transcript variant X3, mRNA.

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CloneID	ORa57439
Clone ID Related Accession (Same CDS sequence)	XM_017597469.1
Accession Version	XM_017597469.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase epsilon isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAG ACCAGCGGTC CGGCCCATCG GCGCAGGGCC TGCTCCCCGA TGGCCACTTG 
ATCCTATGGA CGCTGTTCTC CGTGCTGTTG CCGGTGTTCA TCACCTTATG GTGTAGTCTG 
CAGCGTTCGC GCCGGCAGCT GCACCGCAGG GACATCTTCC GCAAGAGCAA GCACTGCTGG 
CGTGACACCG ACCTGTTCAG CCACCCCACC TACTGCTGCG TGTGCGCCCA GCACATCCTG 
CAAGGAGCCT TCTGCGACTG CTGCGGGCTC CGCGTGGACG AGGGCTGCCT CAAGAAGGTC 
GACAAGCGCT TCCCGTGCAA GGAGATCATG CTCAAGAACG ACAGCAGGGC CGCAGATGCG 
ATGCCCCACC ACTGGATCCG CGGCAACGTC CCCCTGTGCA CTTACTGTGT AATCTGCAGG 
CAGCAGTGTG GCAGCCAGCC TAAACTCTGC GACTACCGGT GTGTTTGGTG CCAGCAGACA 
GTCCACGATG AGTGCATGAG AGGCAGCTTA AAGAGTGAGA AGTGTGATTT TGGAGAATTC 
AAAAATCTCA TCATCCCTCC AAGTTATTTA ACGTCCATTA ACCAGATGCG TAAAGACAAA 
AACACAAATT ATGAAGTGCT TGCTTCTAAG TTTGGAAAGC AGTGGACCCC ATTAATAATC 
CTGGCCAACT CTCGTAGTGG AACTAACATG GGAGAAGGAC TGTTGGGAGA GTTTAAGATG 
CTGTTAAATC CCGTCCAGGT GTTCGATGTC ACTAAAACTC CTCCGATCAA AGCTCTGCAG 
CTGTGCACTC TTCTTCCTTA TTACTCCGTT CGAGTCCTTG TTTGTGGAGG GGATGGGACT 
GTGGGCTGGG TCCTCGATGC AATTGATGAA ATGAAGATTA AGGGACAAGA AAAATACATT 
CCAGAGGTTG CAGTCTTACC TCTGGGAACA GGCAACGACC TATCCAACAC CCTGGGCTGG 
GGTACTGGCT ATGCTGGAGA AATCCCGGTT GCACAGGTCT TAAGGAATGT AATGGAAGCA 
GATGGAATCA AACTAGACAG ATGGAAAGTG CAGGTAACAA ATAAAGGCTA CTACAGTTTA 
AGGAAACCCA AGGAGTTCAC AATGAACAAC TACTTCTCTA TTGGACCCGA TGCCCTCATG 
GCTCTCAATT TTCATGCTCA TCGTGAGAAG GCGCCGTCTC TGTTTTCCAG CCGAATCCTT 
AACAAGGCTG TTTATTTATT CTATGGAACT AGAGATTGCT TGGTGCAAGA GTGTAAAGAT 
TTGAATAAAA AAATTGAGCT AGAGCTGGAT GGTGAGCGAG TTGAACTGCC TAACTTGGAG 
GGAGTTATTG TTCTGAACAT TGGCTACTGG GGCGGTGGCT GCCGACTGTG GGAAGGAGTG 
GGAGATGAGA CGTATCCTCT AGCCAGATGT GCCCCAGGGG TTCCTGCTTT GAATGCTTGC 
TCTCTGTCTG GTCTCACTAT TTTGGGAAGT TGTGAAACCA TTAGTTCATG TTTACTAGCA 
TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452958.1
CDS	405..1910
Translation	MEGDQRSGPSAQGLLPDGHLILWTLFSVLLPVFITLWCSLQRSRRQLHRRDIFRKSKHCWRDTDLFSHPTYCCVCAQHILQGAFCDCCGLRVDEGCLKKVDKRFPCKEIMLKNDSRAADAMPHHWIRGNVPLCTYCVICRQQCGSQPKLCDYRCVWCQQTVHDECMRGSLKSEKCDFGEFKNLIIPPSYLTSINQMRKDKNTNYEVLASKFGKQWTPLIILANSRSGTNMGEGLLGEFKMLLNPVQVFDVTKTPPIKALQLCTLLPYYSVRVLVCGGDGTVGWVLDAIDEMKIKGQEKYIPEVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGIKLDRWKVQVTNKGYYSLRKPKEFTMNNYFSIGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTRDCLVQECKDLNKKIELELDGERVELPNLEGVIVLNIGYWGGGCRLWEGVGDETYPLARCAPGVPALNACSLSGLTILGSCETISSCLLAC

Target ORF information:

RefSeq Version	XM_017597469.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase epsilon (Dgke), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597469.1

ORF Insert Sequence:

ATGGAGGGAG ACCAGCGGTC CGGCCCATCG GCGCAGGGCC TGCTCCCCGA TGGCCACTTG 
ATCCTATGGA CGCTGTTCTC CGTGCTGTTG CCGGTGTTCA TCACCTTATG GTGTAGTCTG 
CAGCGTTCGC GCCGGCAGCT GCACCGCAGG GACATCTTCC GCAAGAGCAA GCACTGCTGG 
CGTGACACCG ACCTGTTCAG CCACCCCACC TACTGCTGCG TGTGCGCCCA GCACATCCTG 
CAAGGAGCCT TCTGCGACTG CTGCGGGCTC CGCGTGGACG AGGGCTGCCT CAAGAAGGTC 
GACAAGCGCT TCCCGTGCAA GGAGATCATG CTCAAGAACG ACAGCAGGGC CGCAGATGCG 
ATGCCCCACC ACTGGATCCG CGGCAACGTC CCCCTGTGCA CTTACTGTGT AATCTGCAGG 
CAGCAGTGTG GCAGCCAGCC TAAACTCTGC GACTACCGGT GTGTTTGGTG CCAGCAGACA 
GTCCACGATG AGTGCATGAG AGGCAGCTTA AAGAGTGAGA AGTGTGATTT TGGAGAATTC 
AAAAATCTCA TCATCCCTCC AAGTTATTTA ACGTCCATTA ACCAGATGCG TAAAGACAAA 
AACACAAATT ATGAAGTGCT TGCTTCTAAG TTTGGAAAGC AGTGGACCCC ATTAATAATC 
CTGGCCAACT CTCGTAGTGG AACTAACATG GGAGAAGGAC TGTTGGGAGA GTTTAAGATG 
CTGTTAAATC CCGTCCAGGT GTTCGATGTC ACTAAAACTC CTCCGATCAA AGCTCTGCAG 
CTGTGCACTC TTCTTCCTTA TTACTCCGTT CGAGTCCTTG TTTGTGGAGG GGATGGGACT 
GTGGGCTGGG TCCTCGATGC AATTGATGAA ATGAAGATTA AGGGACAAGA AAAATACATT 
CCAGAGGTTG CAGTCTTACC TCTGGGAACA GGCAACGACC TATCCAACAC CCTGGGCTGG 
GGTACTGGCT ATGCTGGAGA AATCCCGGTT GCACAGGTCT TAAGGAATGT AATGGAAGCA 
GATGGAATCA AACTAGACAG ATGGAAAGTG CAGGTAACAA ATAAAGGCTA CTACAGTTTA 
AGGAAACCCA AGGAGTTCAC AATGAACAAC TACTTCTCTA TTGGACCCGA TGCCCTCATG 
GCTCTCAATT TTCATGCTCA TCGTGAGAAG GCGCCGTCTC TGTTTTCCAG CCGAATCCTT 
AACAAGGCTG TTTATTTATT CTATGGAACT AGAGATTGCT TGGTGCAAGA GTGTAAAGAT 
TTGAATAAAA AAATTGAGCT AGAGCTGGAT GGTGAGCGAG TTGAACTGCC TAACTTGGAG 
GGAGTTATTG TTCTGAACAT TGGCTACTGG GGCGGTGGCT GCCGACTGTG GGAAGGAGTG 
GGAGATGAGA CGTATCCTCT AGCCAGATGT GCCCCAGGGG TTCCTGCTTT GAATGCTTGC 
TCTCTGTCTG GTCTCACTAT TTTGGGAAGT TGTGAAACCA TTAGTTCATG TTTACTAGCA 
TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Diacylglycerol kinase ? localizes to subsurface cisterns of cerebellar Purkinje cells.
Cell and tissue research368(3)441-458(2017 06)
Hozumi Y,Fujiwara H,Kaneko K,Fujii S,Topham MK,Watanabe M,Goto K

Distinct expression and localization of diacylglycerol kinase isozymes in rat retina.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society61(6)462-76(2013 Jun)
Hozumi Y,Matsui H,Sakane F,Watanabe M,Goto K

Recessive mutations in DGKE cause atypical hemolytic-uremic syndrome.
Nature genetics45(5)531-6(2013 May)
Lemaire M,Fr?meaux-Bacchi V,Schaefer F,Choi M,Tang WH,Le Quintrec M,Fakhouri F,Taque S,Nobili F,Martinez F,Ji W,Overton JD,Mane SM,N?rnberg G,Altm?ller J,Thiele H,Morin D,Deschenes G,Baudouin V,Llanas B,Collard L,Majid MA,Simkova E,N?rnberg P,Rioux-Leclerc N,Moeckel GW,Gubler MC,Hwa J,Loirat C,Lifton RP

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Localization of diacylglycerol kinase epsilon on stress fibers in vascular smooth muscle cells.
Cell and tissue research337(1)167-75(2009 Jul)
Nakano T,Hozumi Y,Goto K,Wakabayashi I

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Dgke cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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