Mthfd1l cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mthfd1l
Entrez Gene ID	361472
Full Name	methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
Synonyms	Fthfsdc1
General protein information	Preferred Names methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like Names monofunctional C1-tetrahydrofolate synthase, mitochondrial formyltetrahydrofolate synthetase domain containing 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1p11-q11

mRNA and Protein(s)

mRNA	Protein	Name
NM_001108462.2	NP_001101932.2	monofunctional C1-tetrahydrofolate synthase, mitochondrial

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MTHFD1L	XP_518807.3
	MTHFD1L	XP_001098522.2
Danio rerio (zebrafish)	mthfd1l	NP_001229925.1
Homo sapiens (human)	MTHFD1L	NP_001229696.1
Rattus norvegicus (Norway rat)	Mthfd1l	NP_001101932.2
Canis lupus familiaris (dog)	MTHFD1L	XP_533450.2
Bos taurus (cattle)	MTHFD1L	NP_001069486.1
Gallus gallus (chicken)	MTHFD1L	XP_004935675.1
Mus musculus (house mouse)	Mthfd1l	NP_758512.3
Xenopus tropicalis (tropical clawed frog)	mthfd1l	NP_001120171.1

Gene Ontology
Bibliography

Related articles in PubMed

Deletion of Mthfd1l causes embryonic lethality and neural tube and craniofacial defects in mice.
Momb J, Lewandowski JP, Bryant JD, Fitch R, Surman DR, Vokes SA, Appling DR
Proceedings of the National Academy of Sciences of the United States of America110(2)549-54(2013 Jan)

Update and new concepts in vitamin responsive disorders of folate transport and metabolism.
Watkins D, Rosenblatt DS
Journal of inherited metabolic disease35(4)665-70(2012 Jul)

Mitochondrial C1-tetrahydrofolate synthase (MTHFD1L) supports the flow of mitochondrial one-carbon units into the methyl cycle in embryos.
Pike ST, Rajendra R, Artzt K, Appling DR
The Journal of biological chemistry285(7)4612-20(2010 Feb)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

A common variant in MTHFD1L is associated with neural tube defects and mRNA splicing efficiency.
Parle-McDermott A, Pangilinan F, O'Brien KK, Mills JL, Magee AM, Troendle J, Sutton M, Scott JM, Kirke PN, Molloy AM, Brody LC
Human mutation30(12)1650-6(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mthfd1l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mthfd1l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00354	NM_001108462.2 Latest version!	Rattus norvegicus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$188.30 $269.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mthfd1l ( NM_001108462.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101932.2 Rattus norvegicus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), mRNA.

Price & Availability↑

CloneID	ORa00354
Clone ID Related Accession (Same CDS sequence)	NM_001108462.2
Accession Version	NM_001108462.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Rattus norvegicus(Norway rat)
Product	monofunctional C1-tetrahydrofolate synthase, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC166462.1. On Dec 2, 2010 this sequence version replaced NM_001108462.1. ##Evidence-Data-START## Transcript exon combination :: BC166462.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGAGTGCCC GCCTTCCTCT CCTGCTACGC CAACTCGGCC ACCAGCAGCT GCCCCCGGGC 
CCGGCGTGTC GCCTCCGTGA GCTGTGTCGA TCCGGCAGCA GAAGCAGCAG CAGCGGAGGT 
GGTGACCCCG AGGGGTTGCG CGGGCGGCGG CTGCAGGATG GCCAAGCCTT CATCAGCCAT 
AGCCCAGGCA GTCCGGAAGA CCCCGGGATG GACTCAATCG TTAGAGACGT CATTCACAAT 
TCCAAAGAAG TGCTGAACTT GTTGCAAGAA AAAAACCCTG CCTTTAAGCC AGTTCTTGCT 
GTGATTCAGG CAGGAGATGA CCACCTGATG CAGAATGTGA ACCAGAATTT GGCCAAGGAG 
GCTGGCCTGG ACATCACTCA CATCTGCCTA CCTCCAGACA GTGGAGAGGA TGAGATCATA 
GATGAAATCC TGAAGATTAA TGAAGACCCT AGAGTGCATG GCCTTGCCCT TCAGATCTCT 
GAAGATTCAT TGTGCAACAA AGTTCTCAAT GCTTTGAAAC CGGAAAAAGA TGTGGACGGG 
GTGACCGATG TCAATCTAGG GAAGCTGGTG CGAGGTGACG CTCCTGAATG TTTTGTTTCG 
CCCTTGGCCA GAGCTGCAGT AGAACTCATT GAGAAATCAG GGATCACCTT GGATGGAAAG 
AAGGTCTTGG TCATAGGGGC TCACGGGCCT CTGGAAGCTG CCCTGCAATG TCTTTTCCAG 
AGAAAAGGAT CCATGACAAT GAGCTCCCCG TGGATAACAC CTCAGCTTCA AGACAAGCTT 
CGAGAGGCAG ACATTGTGGT CTTGGGCTCA CCTAAGCCCG ACGAAATTCC TGCTGCTTGG 
ATCCCGTCAG GAGCCACCAT GCTCAATTGC TTCCATGACT TCTTGTCAGG GAAGCTGAGA 
GGAGGTTCTC CTGGGGTCCC CATGAACAGA CTCATTGCCG ACGAGAGCGT GAGTCTCCTT 
GCTGCAGCTC TGCGGGTTCA GAACATGGTG AACAGTGGCA GGAGATGGCT CCGAGAGCAG 
CAGCATAGAA GATGGCGACT CCACTGCTTG AAACTGCAGC CTCTCTCCCC TGTGCCCAGT 
GATATTGAGA TCTCACGAGG ACAGACTCCA AAAGCGGTGG ATGTCCTTGC CAAGGAGATA 
GGATTGCTTG CAGATGAAAT TGAAATCTAC GGCAAGAGCA AGGCCAAAGT CCGCTTGTCC 
CTGCTGGAGA GGTTAAAGGA TCAAACAGAT GGAAAATACG TCTTGGTTGC TGGGATCACA 
CCCACCCCAC TTGGAGAAGG GAAGAGCACA GTCACCATCG GACTAGTGCA GGCACTAACT 
GCTCACCTGA ACGTCAACTC CTTCGCCTGT CTGAGACAGC CTTCCCAGGG ACCCACTTTT 
GGAGTGAAAG GAGGAGCTGC AGGTGGTGGA TATGCTCAGG TCATTCCCAT GGAGGAGTTC 
AACCTTCACC TGACTGGGGA CATCCACGCC ATCACTGCCG CTAATAACTT ACTGGCTGCT 
GCCATCGACA CGAGAATTTT CCATGAGAGC ACTCAGACAG ACAAGGCTCT ATACAATCGA 
CTGGTTCCCT TAGTGAATGG TGTCAGAAAA TTTTCAGAAA TTCAACTTTC TCGGCTGAAG 
AAACTGGGGA TACATAAGAC TGATCCCACC GCTCTGACAG AAGAGGAAGT GAGGAAGTTC 
ACCCGCCTCA ACATTGACCC CTCTACCATC ACATGGCAGA GAGTATTGGA CACGAATGAC 
CGATTTCTGA GAAAAATAAC TATTGGGCAG GGAAGCACGG AGAAAGGATA TTCCCGGCAG 
GCACAGTTTG ACATTGCAGT GGCCAGCGAG ATCATGGCCG TGCTGGCCTT GACTGATAGC 
CTCGCAGACA TGAAAGAGCG GCTAGGAAGA ATGGTGGTGG CCAGTGACAA AGATGGGCAG 
CCTGTGACAG CTGAGGACTT GGGGGTGACA GGTGCTCTGA CAGTTTTAAT GAAAGACGCC 
ATCAAGCCAA ATCTAATGCA GACCCTGGAA GGAACACCTG TGTTTGTGCA CGCTGGTCCC 
TTTGCTAATA TCGCTCATGG CAACTCGTCG GTGTTAGCTG ATAAAATCGC ACTAAAACTG 
GTCGGCGAAG AAGGGTTTGT AGTGACTGAA GCTGGCTTTG GGGCTGATAT TGGGATGGAG 
AAATTCTTCA ACATCAAATG CCGAGCTTCT GGCCTGGTGC CCAATGTTGT TGTCCTGGTG 
GCCACCGTTC GAGCTCTGAA GATGCATGGA GGTGGGCCAA GTGTAACCGC TGGGGTTCCT 
CTCAAGAAAG AATATACAGA AGAGAACATT CAGCTGGTAG CTGATGGCTG CTGCAACCTA 
CAGAAACAAA TTCAGATCGC TCAGCTCTTC GGGGTGCCCG TGGTGGTGGC ACTGAATGTC 
TTCAAGACGG ACACCCGTGC AGAGATTGAC CTGGTGTGTG AGCTCGCTAA ACGGGCCGGT 
GCCTTTGATG CGGTCCCCTG CTATCACTGG TCAGCTGGAG GAAAAGGGTC CGTGGACTTG 
GCTCGGGCTG TGAGAGAAGC TGCGAATAAG AGAAGCCGCT TCCGGTTCCT GTACGATGTT 
CAGCTTCCCA TCGTGGCAAA GATAAGAGTA ATTGCCCAGA CCGTCTATGG AGCCAAGGAT 
ATTGAGCTGT CTCCTGAGGC ACAGGCCAAA ATCGACCGCT ATACGCAACA GGGTTTTGGA 
AATTTGCCCA TCTGCATGGC AAAGACCCAC CTTTCTCTGT CTCATGAACC TGACAAAAAG 
GGGGTGCCGA GGGACTTCAT TCTGCCCATC AGTGATGTCC GAGCCAGCAT CGGGGCTGGG 
TTCATTTACC CTTTGGTGGG AATGATGAGC ACAATGCCAG GTCTGCCCAC CCGACCCTGC 
TTTTACGATA TAGACCTTGA CACAGAAACA GAGCAAGTTA AAGGCTTGTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101932.2
CDS	106..3039
Translation	MSARLPLLLRQLGHQQLPPGPACRLRELCRSGSRSSSSGGGDPEGLRGRRLQDGQAFISHSPGSPEDPGMDSIVRDVIHNSKEVLNLLQEKNPAFKPVLAVIQAGDDHLMQNVNQNLAKEAGLDITHICLPPDSGEDEIIDEILKINEDPRVHGLALQISEDSLCNKVLNALKPEKDVDGVTDVNLGKLVRGDAPECFVSPLARAAVELIEKSGITLDGKKVLVIGAHGPLEAALQCLFQRKGSMTMSSPWITPQLQDKLREADIVVLGSPKPDEIPAAWIPSGATMLNCFHDFLSGKLRGGSPGVPMNRLIADESVSLLAAALRVQNMVNSGRRWLREQQHRRWRLHCLKLQPLSPVPSDIEISRGQTPKAVDVLAKEIGLLADEIEIYGKSKAKVRLSLLERLKDQTDGKYVLVAGITPTPLGEGKSTVTIGLVQALTAHLNVNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLLAAAIDTRIFHESTQTDKALYNRLVPLVNGVRKFSEIQLSRLKKLGIHKTDPTALTEEEVRKFTRLNIDPSTITWQRVLDTNDRFLRKITIGQGSTEKGYSRQAQFDIAVASEIMAVLALTDSLADMKERLGRMVVASDKDGQPVTAEDLGVTGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSVLADKIALKLVGEEGFVVTEAGFGADIGMEKFFNIKCRASGLVPNVVVLVATVRALKMHGGGPSVTAGVPLKKEYTEENIQLVADGCCNLQKQIQIAQLFGVPVVVALNVFKTDTRAEIDLVCELAKRAGAFDAVPCYHWSAGGKGSVDLARAVREAANKRSRFRFLYDVQLPIVAKIRVIAQTVYGAKDIELSPEAQAKIDRYTQQGFGNLPICMAKTHLSLSHEPDKKGVPRDFILPISDVRASIGAGFIYPLVGMMSTMPGLPTRPCFYDIDLDTETEQVKGLF

Target ORF information:

RefSeq Version	NM_001108462.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108462.2

ORF Insert Sequence:

ATGAGTGCCC GCCTTCCTCT CCTGCTACGC CAACTCGGCC ACCAGCAGCT GCCCCCGGGC 
CCGGCGTGTC GCCTCCGTGA GCTGTGTCGA TCCGGCAGCA GAAGCAGCAG CAGCGGAGGT 
GGTGACCCCG AGGGGTTGCG CGGGCGGCGG CTGCAGGATG GCCAAGCCTT CATCAGCCAT 
AGCCCAGGCA GTCCGGAAGA CCCCGGGATG GACTCAATCG TTAGAGACGT CATTCACAAT 
TCCAAAGAAG TGCTGAACTT GTTGCAAGAA AAAAACCCTG CCTTTAAGCC AGTTCTTGCT 
GTGATTCAGG CAGGAGATGA CCACCTGATG CAGAATGTGA ACCAGAATTT GGCCAAGGAG 
GCTGGCCTGG ACATCACTCA CATCTGCCTA CCTCCAGACA GTGGAGAGGA TGAGATCATA 
GATGAAATCC TGAAGATTAA TGAAGACCCT AGAGTGCATG GCCTTGCCCT TCAGATCTCT 
GAAGATTCAT TGTGCAACAA AGTTCTCAAT GCTTTGAAAC CGGAAAAAGA TGTGGACGGG 
GTGACCGATG TCAATCTAGG GAAGCTGGTG CGAGGTGACG CTCCTGAATG TTTTGTTTCG 
CCCTTGGCCA GAGCTGCAGT AGAACTCATT GAGAAATCAG GGATCACCTT GGATGGAAAG 
AAGGTCTTGG TCATAGGGGC TCACGGGCCT CTGGAAGCTG CCCTGCAATG TCTTTTCCAG 
AGAAAAGGAT CCATGACAAT GAGCTCCCCG TGGATAACAC CTCAGCTTCA AGACAAGCTT 
CGAGAGGCAG ACATTGTGGT CTTGGGCTCA CCTAAGCCCG ACGAAATTCC TGCTGCTTGG 
ATCCCGTCAG GAGCCACCAT GCTCAATTGC TTCCATGACT TCTTGTCAGG GAAGCTGAGA 
GGAGGTTCTC CTGGGGTCCC CATGAACAGA CTCATTGCCG ACGAGAGCGT GAGTCTCCTT 
GCTGCAGCTC TGCGGGTTCA GAACATGGTG AACAGTGGCA GGAGATGGCT CCGAGAGCAG 
CAGCATAGAA GATGGCGACT CCACTGCTTG AAACTGCAGC CTCTCTCCCC TGTGCCCAGT 
GATATTGAGA TCTCACGAGG ACAGACTCCA AAAGCGGTGG ATGTCCTTGC CAAGGAGATA 
GGATTGCTTG CAGATGAAAT TGAAATCTAC GGCAAGAGCA AGGCCAAAGT CCGCTTGTCC 
CTGCTGGAGA GGTTAAAGGA TCAAACAGAT GGAAAATACG TCTTGGTTGC TGGGATCACA 
CCCACCCCAC TTGGAGAAGG GAAGAGCACA GTCACCATCG GACTAGTGCA GGCACTAACT 
GCTCACCTGA ACGTCAACTC CTTCGCCTGT CTGAGACAGC CTTCCCAGGG ACCCACTTTT 
GGAGTGAAAG GAGGAGCTGC AGGTGGTGGA TATGCTCAGG TCATTCCCAT GGAGGAGTTC 
AACCTTCACC TGACTGGGGA CATCCACGCC ATCACTGCCG CTAATAACTT ACTGGCTGCT 
GCCATCGACA CGAGAATTTT CCATGAGAGC ACTCAGACAG ACAAGGCTCT ATACAATCGA 
CTGGTTCCCT TAGTGAATGG TGTCAGAAAA TTTTCAGAAA TTCAACTTTC TCGGCTGAAG 
AAACTGGGGA TACATAAGAC TGATCCCACC GCTCTGACAG AAGAGGAAGT GAGGAAGTTC 
ACCCGCCTCA ACATTGACCC CTCTACCATC ACATGGCAGA GAGTATTGGA CACGAATGAC 
CGATTTCTGA GAAAAATAAC TATTGGGCAG GGAAGCACGG AGAAAGGATA TTCCCGGCAG 
GCACAGTTTG ACATTGCAGT GGCCAGCGAG ATCATGGCCG TGCTGGCCTT GACTGATAGC 
CTCGCAGACA TGAAAGAGCG GCTAGGAAGA ATGGTGGTGG CCAGTGACAA AGATGGGCAG 
CCTGTGACAG CTGAGGACTT GGGGGTGACA GGTGCTCTGA CAGTTTTAAT GAAAGACGCC 
ATCAAGCCAA ATCTAATGCA GACCCTGGAA GGAACACCTG TGTTTGTGCA CGCTGGTCCC 
TTTGCTAATA TCGCTCATGG CAACTCGTCG GTGTTAGCTG ATAAAATCGC ACTAAAACTG 
GTCGGCGAAG AAGGGTTTGT AGTGACTGAA GCTGGCTTTG GGGCTGATAT TGGGATGGAG 
AAATTCTTCA ACATCAAATG CCGAGCTTCT GGCCTGGTGC CCAATGTTGT TGTCCTGGTG 
GCCACCGTTC GAGCTCTGAA GATGCATGGA GGTGGGCCAA GTGTAACCGC TGGGGTTCCT 
CTCAAGAAAG AATATACAGA AGAGAACATT CAGCTGGTAG CTGATGGCTG CTGCAACCTA 
CAGAAACAAA TTCAGATCGC TCAGCTCTTC GGGGTGCCCG TGGTGGTGGC ACTGAATGTC 
TTCAAGACGG ACACCCGTGC AGAGATTGAC CTGGTGTGTG AGCTCGCTAA ACGGGCCGGT 
GCCTTTGATG CGGTCCCCTG CTATCACTGG TCAGCTGGAG GAAAAGGGTC CGTGGACTTG 
GCTCGGGCTG TGAGAGAAGC TGCGAATAAG AGAAGCCGCT TCCGGTTCCT GTACGATGTT 
CAGCTTCCCA TCGTGGCAAA GATAAGAGTA ATTGCCCAGA CCGTCTATGG AGCCAAGGAT 
ATTGAGCTGT CTCCTGAGGC ACAGGCCAAA ATCGACCGCT ATACGCAACA GGGTTTTGGA 
AATTTGCCCA TCTGCATGGC AAAGACCCAC CTTTCTCTGT CTCATGAACC TGACAAAAAG 
GGGGTGCCGA GGGACTTCAT TCTGCCCATC AGTGATGTCC GAGCCAGCAT CGGGGCTGGG 
TTCATTTACC CTTTGGTGGG AATGATGAGC ACAATGCCAG GTCTGCCCAC CCGACCCTGC 
TTTTACGATA TAGACCTTGA CACAGAAACA GAGCAAGTTA AAGGCTTGTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deletion of Mthfd1l causes embryonic lethality and neural tube and craniofacial defects in mice.
Proceedings of the National Academy of Sciences of the United States of America110(2)549-54(2013 Jan)
Momb J,Lewandowski JP,Bryant JD,Fitch R,Surman DR,Vokes SA,Appling DR

Update and new concepts in vitamin responsive disorders of folate transport and metabolism.
Journal of inherited metabolic disease35(4)665-70(2012 Jul)
Watkins D,Rosenblatt DS

Mitochondrial C1-tetrahydrofolate synthase (MTHFD1L) supports the flow of mitochondrial one-carbon units into the methyl cycle in embryos.
The Journal of biological chemistry285(7)4612-20(2010 Feb)
Pike ST,Rajendra R,Artzt K,Appling DR

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

A common variant in MTHFD1L is associated with neural tube defects and mRNA splicing efficiency.
Human mutation30(12)1650-6(2009 Dec)
Parle-McDermott A,Pangilinan F,O'Brien KK,Mills JL,Magee AM,Troendle J,Sutton M,Scott JM,Kirke PN,Molloy AM,Brody LC

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Mthfd1l cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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