Mthfd1l cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mthfd1l
Entrez Gene ID	270685
Full Name	methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
Synonyms	2410004L15Rik,AI647056,Fthfsdc1
General protein information	Preferred Names methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like Names monofunctional C1-tetrahydrofolate synthase, mitochondrial formyltetrahydrofolate synthetase domain containing 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 A1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006512449.3	XP_006512512.1	monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X1
NM_001170785.1	NP_001164256.1	monofunctional C1-tetrahydrofolate synthase, mitochondrial precursor
NM_001170786.1	NP_001164257.1	monofunctional C1-tetrahydrofolate synthase, mitochondrial precursor
NM_172308.4	NP_758512.3	monofunctional C1-tetrahydrofolate synthase, mitochondrial precursor
XM_006512449.4	XP_006512512.1	monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MTHFD1L	XP_518807.3
	MTHFD1L	XP_001098522.2
Danio rerio (zebrafish)	mthfd1l	NP_001229925.1
Homo sapiens (human)	MTHFD1L	NP_001229696.1
Rattus norvegicus (Norway rat)	Mthfd1l	NP_001101932.2
Canis lupus familiaris (dog)	MTHFD1L	XP_533450.2
Bos taurus (cattle)	MTHFD1L	NP_001069486.1
Gallus gallus (chicken)	MTHFD1L	XP_004935675.1
Mus musculus (house mouse)	Mthfd1l	NP_758512.3
Xenopus tropicalis (tropical clawed frog)	mthfd1l	NP_001120171.1

Gene Ontology
Bibliography

Related articles in PubMed

Mitochondrial MTHFD2L is a dual redox cofactor-specific methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase expressed in both adult and embryonic tissues.
Shin M, Bryant JD, Momb J, Appling DR
The Journal of biological chemistry289(22)15507-17(2014 May)

Deletion of Mthfd1l causes embryonic lethality and neural tube and craniofacial defects in mice.
Momb J, Lewandowski JP, Bryant JD, Fitch R, Surman DR, Vokes SA, Appling DR
Proceedings of the National Academy of Sciences of the United States of America110(2)549-54(2013 Jan)

Mitochondrial C1-tetrahydrofolate synthase (MTHFD1L) supports the flow of mitochondrial one-carbon units into the methyl cycle in embryos.
Pike ST, Rajendra R, Artzt K, Appling DR
The Journal of biological chemistry285(7)4612-20(2010 Feb)

Agouti C57BL/6N embryonic stem cells for mouse genetic resources.
Pettitt SJ, Liang Q, Rairdan XY, Moran JL, Prosser HM, Beier DR, Lloyd KC, Bradley A, Skarnes WC
Nature methods6(7)493-5(2009 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Deletion of Mthfd1l causes embryonic lethality and neural tube and craniofacial defects in mice.
Title: Deletion of Mthfd1l causes embryonic lethality and neural tube and craniofacial defects in mice.

Data show that the MTHFD1L enzyme is present in mitochondria from normal embryonic tissues and embryonic fibroblast cell lines, and embryonic mitochondria possess the ability to synthesize formate from glycine.
Title: Mitochondrial C1-tetrahydrofolate synthase (MTHFD1L) supports the flow of mitochondrial one-carbon units into the methyl cycle in embryos.

The following Mthfd1l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mthfd1l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15583	NM_172308.4 Latest version!	Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu15583	NM_001170785.1 Latest version!	Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu15583	NM_001170786.1 Latest version!	Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu43654	XM_006512449.4 Latest version!	Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu43654	XM_006512449.3	Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mthfd1l ( NM_001170785.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001164256.1 Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15583
Clone ID Related Accession (Same CDS sequence)	NM_001170785.1 , NM_001170786.1 , NM_172308.4
Accession Version	NM_001170785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	monofunctional C1-tetrahydrofolate synthase, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC153570.9, AK036284.1 and AC159148.10. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK036284.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849388, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGAGCGTCC GCCTCCCTCT CCTGCTTCGC CAGCTCGGCC GCCAGCAGCT GCCCTCGGGC 
CCGGCGTGTC GCCTCCGTGA GCTGTGTCGC TCAGGCAGCA GAAGCAGCAG CAGCGGAGGT 
GGTGACCCCG AGGGGTTACG CGGGCGGCGG CTGCAGGATG GCCAAACCTT CAGCAGCCAT 
GGCCCAGGCA ATCCGGAAGC CCCCGGGATG GACTCCATCG TCAGAGACGT CATTCACAAT 
TCCAAGGAAG TGCTGAGCTT GTTGCAAGAA AAGAACCCTG CCTTTAAGCC AGTGCTTGTG 
GTTATTCAGG CCGGTGATGA CAATTTGATG AAGGATATGA ACCAGAATTT GGCCAAGGAG 
GCTGGTCTGG ACATCACTCA CATCTGCTTG CCTCCAGACA GCGGAGAGGA TGAGATCATA 
GATGAAATCC TGAAGATTAA TGAAGACCCC AGAGTGCATG GCCTTACCCT TCAGATCTCT 
GAAGATTCAT TGAGCAACAA AGTTCTCAAT GCTTTGAAAC CAGAAAAAGA TGTGGACGGG 
GTGACCGACA TCAATCTGGG GAAGCTCGTA CGAGGTGACG CTCCGGAGTG TTTTGTTTCA 
CCCTTGGCCA AAGCTGCTGT AGAACTTGTT GAAAAGTCAG GGATCACTTT GGATGGAAAG 
AAGGTCTTGG TCGTAGGGGC GCACGGGCCC CTGGAAGCTG CCCTGCAGTG GCTTTTCCAG 
AGAAAAGGAT CTATGACAAT GAGCTGCCCC TGGGCAACAC CTCAGCTTCC AGACAAGCTT 
CGAGAGGCAG ACATTGTGGT CTTAGGCTCA CCTAAGCCAG AAGAGATTCC TGCTGTGTGG 
ATTCCATCAG GAACCACCAT TCTCAATTGC TTCCATGACT TCTTGTCAGG GAAGCTGAGC 
GGAGGTTCTC CTGGGGTCCC CGTGGACAAA CTCATTGCAG AAGAGAGCGT GAGTCTCCTT 
GCTGCGGCTC TGCGCATTCA GAACATGGTG AGCAGCGGCA GGAGGTGGCT CAGAGAGCAG 
CAACATCGAA GATGGCGACT GCACTGCTTG AAACTGCAGC CTCTCTCACC TGTGCCCAGT 
GATATTGAGA TTTCTCGAGG ACAGACTCCA AAAGCTGTGG ATGTCCTTGC CAAGGAGATA 
GGATTGCTTG CAGATGAAAT TGAAATCTAT GGCAAGAGCA AAGCCAAAGT CCAATTGTCC 
CTGCTGGAGA GGTTAAAGGA TCAAACAGAT GGAAAATACG TCTTGGTTGC TGGGATCACG 
CCCACCCCGC TCGGAGAAGG GAAGAGTACA GTCACCATCG GACTAGTGCA GGCGCTGACC 
GCTCACCTGA AAGTCAACTC CTTCGCCTGT CTGAGGCAGC CTTCTCAGGG ACCCACTTTT 
GGAGTGAAAG GAGGAGCTGC AGGAGGTGGA TATGCTCAGG TCATCCCCAT GGAGGAGTTC 
AACCTTCACC TGACTGGGGA CATCCATGCC ATCACTGCCG CTAATAACTT GCTGGCCGCA 
GCCATCGACA CGAGAATTTT ACATGAGAGC ACTCAAACAG ACAAGGCTCT CTACAATCGA 
CTGGTTCCCT TAGTGAATGG TGTCAGAGAA TTTTCAGAAA TTCAACTTTC TCGGCTGAAG 
AAACTGGGGA TACATAAGAC TGATCCAAGC ACACTGACCG AGGAGGAAGT GAGGAAGTTC 
GCCCGCCTCA ACATTGACCC CGCAACCATC ACGTGGCAGA GAGTATTGGA CACGAACGAC 
CGATTTCTGA GAAAAATAAC CATTGGACAG GGAAGTACAG AGAAAGGATA TTCCCGGCAG 
GCACAGTTTG ACATTGCAGT GGCCAGCGAG ATCATGGCCG TGCTGGCCTT GACTGATAGC 
CTCACAGACA TGAAAGAGCG GCTAGGAAGA ATGGTGGTGG CCAGTGACAA AGACGGGCAG 
CCTGTGACAG CTGAGGACTT GGGGGTGACC GGCGCTCTGA CAGTTTTGAT GAAAGACGCC 
ATCAAGCCAA ATCTGATGCA GACCCTTGAA GGAACACCTG TGTTTGTGCA CGCTGGTCCC 
TTTGCCAACA TCGCTCACGG CAACTCATCA GTATTAGCTG ATAAAATCGC GCTGAAATTG 
GTCGGCGAAG AAGGGTTTGT GGTGACTGAA GCTGGCTTTG GGGCTGATAT TGGGATGGAG 
AAATTCTTCA ATATCAAATG CCGAGCTTCT GGCCTGGTGC CCAACGTTGT CGTGCTGGTA 
GCCACCGTTC GAGCTCTGAA GATGCATGGA GGCGGGCCGA GTGTAACTGC TGGGGTTCCT 
CTCAAGAAAG AATATACAGA GGAGAACATT CAGCTGGTAG CTGATGGCTG CTGCAACCTA 
CAGAAGCAAA TTCAGATCGC TCAGCTCTTC GGGGTGCCGG TGGTGGTGGC ACTGAATGTC 
TTCAAGACGG ACACCCGTGC AGAGATTGAC TTGGTGTGTG AGCTCGCTAA ACGGGCCGGT 
GCCTTTGATG CGGTCCCCTG CTATCACTGG TCGGCTGGAG GGAAAGGATC TGTGGACTTG 
GCCCGGGCTG TGAGAGAAGC TGCGAATAAA AGAAGCCGCT TCCAGTTCCT GTATGATGTT 
CAGCTTCCCA TCGTGGAAAA GATAAGAGTC ATTGCCCAGA CTGTCTATGG AGCCAAGGAC 
ATTGAGCTGT CTCCTGAGGC GCAGTCCAAA ATCGATCGCT ATACGCAACA GGGTTTTGGA 
AATTTGCCCA TTTGCATGGC GAAGACCCAC CTCTCTCTCT CTCATGAACC TGACAAAAAG 
GGAGTGCCGA GGGATTTCAC TCTGCCCATC AGTGATGTCC GGGCCAGCAT CGGGGCTGGG 
TTCATTTATC CTTTGGTGGG AACGATGAGC ACAATGCCAG GACTGCCCAC CCGACCCTGC 
TTTTATGATA TAGACCTTGA CACAGAAACG GAGCAAGTTA AAGGCTTGTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164256.1
CDS	181..3114
Translation	MSVRLPLLLRQLGRQQLPSGPACRLRELCRSGSRSSSSGGGDPEGLRGRRLQDGQTFSSHGPGNPEAPGMDSIVRDVIHNSKEVLSLLQEKNPAFKPVLVVIQAGDDNLMKDMNQNLAKEAGLDITHICLPPDSGEDEIIDEILKINEDPRVHGLTLQISEDSLSNKVLNALKPEKDVDGVTDINLGKLVRGDAPECFVSPLAKAAVELVEKSGITLDGKKVLVVGAHGPLEAALQWLFQRKGSMTMSCPWATPQLPDKLREADIVVLGSPKPEEIPAVWIPSGTTILNCFHDFLSGKLSGGSPGVPVDKLIAEESVSLLAAALRIQNMVSSGRRWLREQQHRRWRLHCLKLQPLSPVPSDIEISRGQTPKAVDVLAKEIGLLADEIEIYGKSKAKVQLSLLERLKDQTDGKYVLVAGITPTPLGEGKSTVTIGLVQALTAHLKVNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLLAAAIDTRILHESTQTDKALYNRLVPLVNGVREFSEIQLSRLKKLGIHKTDPSTLTEEEVRKFARLNIDPATITWQRVLDTNDRFLRKITIGQGSTEKGYSRQAQFDIAVASEIMAVLALTDSLTDMKERLGRMVVASDKDGQPVTAEDLGVTGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSVLADKIALKLVGEEGFVVTEAGFGADIGMEKFFNIKCRASGLVPNVVVLVATVRALKMHGGGPSVTAGVPLKKEYTEENIQLVADGCCNLQKQIQIAQLFGVPVVVALNVFKTDTRAEIDLVCELAKRAGAFDAVPCYHWSAGGKGSVDLARAVREAANKRSRFQFLYDVQLPIVEKIRVIAQTVYGAKDIELSPEAQSKIDRYTQQGFGNLPICMAKTHLSLSHEPDKKGVPRDFTLPISDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDTETEQVKGLF

Target ORF information:

RefSeq Version	NM_001170785.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170785.1

ORF Insert Sequence:

ATGAGCGTCC GCCTCCCTCT CCTGCTTCGC CAGCTCGGCC GCCAGCAGCT GCCCTCGGGC 
CCGGCGTGTC GCCTCCGTGA GCTGTGTCGC TCAGGCAGCA GAAGCAGCAG CAGCGGAGGT 
GGTGACCCCG AGGGGTTACG CGGGCGGCGG CTGCAGGATG GCCAAACCTT CAGCAGCCAT 
GGCCCAGGCA ATCCGGAAGC CCCCGGGATG GACTCCATCG TCAGAGACGT CATTCACAAT 
TCCAAGGAAG TGCTGAGCTT GTTGCAAGAA AAGAACCCTG CCTTTAAGCC AGTGCTTGTG 
GTTATTCAGG CCGGTGATGA CAATTTGATG AAGGATATGA ACCAGAATTT GGCCAAGGAG 
GCTGGTCTGG ACATCACTCA CATCTGCTTG CCTCCAGACA GCGGAGAGGA TGAGATCATA 
GATGAAATCC TGAAGATTAA TGAAGACCCC AGAGTGCATG GCCTTACCCT TCAGATCTCT 
GAAGATTCAT TGAGCAACAA AGTTCTCAAT GCTTTGAAAC CAGAAAAAGA TGTGGACGGG 
GTGACCGACA TCAATCTGGG GAAGCTCGTA CGAGGTGACG CTCCGGAGTG TTTTGTTTCA 
CCCTTGGCCA AAGCTGCTGT AGAACTTGTT GAAAAGTCAG GGATCACTTT GGATGGAAAG 
AAGGTCTTGG TCGTAGGGGC GCACGGGCCC CTGGAAGCTG CCCTGCAGTG GCTTTTCCAG 
AGAAAAGGAT CTATGACAAT GAGCTGCCCC TGGGCAACAC CTCAGCTTCC AGACAAGCTT 
CGAGAGGCAG ACATTGTGGT CTTAGGCTCA CCTAAGCCAG AAGAGATTCC TGCTGTGTGG 
ATTCCATCAG GAACCACCAT TCTCAATTGC TTCCATGACT TCTTGTCAGG GAAGCTGAGC 
GGAGGTTCTC CTGGGGTCCC CGTGGACAAA CTCATTGCAG AAGAGAGCGT GAGTCTCCTT 
GCTGCGGCTC TGCGCATTCA GAACATGGTG AGCAGCGGCA GGAGGTGGCT CAGAGAGCAG 
CAACATCGAA GATGGCGACT GCACTGCTTG AAACTGCAGC CTCTCTCACC TGTGCCCAGT 
GATATTGAGA TTTCTCGAGG ACAGACTCCA AAAGCTGTGG ATGTCCTTGC CAAGGAGATA 
GGATTGCTTG CAGATGAAAT TGAAATCTAT GGCAAGAGCA AAGCCAAAGT CCAATTGTCC 
CTGCTGGAGA GGTTAAAGGA TCAAACAGAT GGAAAATACG TCTTGGTTGC TGGGATCACG 
CCCACCCCGC TCGGAGAAGG GAAGAGTACA GTCACCATCG GACTAGTGCA GGCGCTGACC 
GCTCACCTGA AAGTCAACTC CTTCGCCTGT CTGAGGCAGC CTTCTCAGGG ACCCACTTTT 
GGAGTGAAAG GAGGAGCTGC AGGAGGTGGA TATGCTCAGG TCATCCCCAT GGAGGAGTTC 
AACCTTCACC TGACTGGGGA CATCCATGCC ATCACTGCCG CTAATAACTT GCTGGCCGCA 
GCCATCGACA CGAGAATTTT ACATGAGAGC ACTCAAACAG ACAAGGCTCT CTACAATCGA 
CTGGTTCCCT TAGTGAATGG TGTCAGAGAA TTTTCAGAAA TTCAACTTTC TCGGCTGAAG 
AAACTGGGGA TACATAAGAC TGATCCAAGC ACACTGACCG AGGAGGAAGT GAGGAAGTTC 
GCCCGCCTCA ACATTGACCC CGCAACCATC ACGTGGCAGA GAGTATTGGA CACGAACGAC 
CGATTTCTGA GAAAAATAAC CATTGGACAG GGAAGTACAG AGAAAGGATA TTCCCGGCAG 
GCACAGTTTG ACATTGCAGT GGCCAGCGAG ATCATGGCCG TGCTGGCCTT GACTGATAGC 
CTCACAGACA TGAAAGAGCG GCTAGGAAGA ATGGTGGTGG CCAGTGACAA AGACGGGCAG 
CCTGTGACAG CTGAGGACTT GGGGGTGACC GGCGCTCTGA CAGTTTTGAT GAAAGACGCC 
ATCAAGCCAA ATCTGATGCA GACCCTTGAA GGAACACCTG TGTTTGTGCA CGCTGGTCCC 
TTTGCCAACA TCGCTCACGG CAACTCATCA GTATTAGCTG ATAAAATCGC GCTGAAATTG 
GTCGGCGAAG AAGGGTTTGT GGTGACTGAA GCTGGCTTTG GGGCTGATAT TGGGATGGAG 
AAATTCTTCA ATATCAAATG CCGAGCTTCT GGCCTGGTGC CCAACGTTGT CGTGCTGGTA 
GCCACCGTTC GAGCTCTGAA GATGCATGGA GGCGGGCCGA GTGTAACTGC TGGGGTTCCT 
CTCAAGAAAG AATATACAGA GGAGAACATT CAGCTGGTAG CTGATGGCTG CTGCAACCTA 
CAGAAGCAAA TTCAGATCGC TCAGCTCTTC GGGGTGCCGG TGGTGGTGGC ACTGAATGTC 
TTCAAGACGG ACACCCGTGC AGAGATTGAC TTGGTGTGTG AGCTCGCTAA ACGGGCCGGT 
GCCTTTGATG CGGTCCCCTG CTATCACTGG TCGGCTGGAG GGAAAGGATC TGTGGACTTG 
GCCCGGGCTG TGAGAGAAGC TGCGAATAAA AGAAGCCGCT TCCAGTTCCT GTATGATGTT 
CAGCTTCCCA TCGTGGAAAA GATAAGAGTC ATTGCCCAGA CTGTCTATGG AGCCAAGGAC 
ATTGAGCTGT CTCCTGAGGC GCAGTCCAAA ATCGATCGCT ATACGCAACA GGGTTTTGGA 
AATTTGCCCA TTTGCATGGC GAAGACCCAC CTCTCTCTCT CTCATGAACC TGACAAAAAG 
GGAGTGCCGA GGGATTTCAC TCTGCCCATC AGTGATGTCC GGGCCAGCAT CGGGGCTGGG 
TTCATTTATC CTTTGGTGGG AACGATGAGC ACAATGCCAG GACTGCCCAC CCGACCCTGC 
TTTTATGATA TAGACCTTGA CACAGAAACG GAGCAAGTTA AAGGCTTGTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mthfd1l ( NM_001170786.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001164257.1 Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu15583
Clone ID Related Accession (Same CDS sequence)	NM_001170785.1 , NM_001170786.1 , NM_172308.4
Accession Version	NM_001170786.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	monofunctional C1-tetrahydrofolate synthase, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC153570.9, AK160025.1 and AC159148.10. Transcript Variant: This variant (3) differs in the 3' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK160025.1, AK075931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCGTCC GCCTCCCTCT CCTGCTTCGC CAGCTCGGCC GCCAGCAGCT GCCCTCGGGC 
CCGGCGTGTC GCCTCCGTGA GCTGTGTCGC TCAGGCAGCA GAAGCAGCAG CAGCGGAGGT 
GGTGACCCCG AGGGGTTACG CGGGCGGCGG CTGCAGGATG GCCAAACCTT CAGCAGCCAT 
GGCCCAGGCA ATCCGGAAGC CCCCGGGATG GACTCCATCG TCAGAGACGT CATTCACAAT 
TCCAAGGAAG TGCTGAGCTT GTTGCAAGAA AAGAACCCTG CCTTTAAGCC AGTGCTTGTG 
GTTATTCAGG CCGGTGATGA CAATTTGATG AAGGATATGA ACCAGAATTT GGCCAAGGAG 
GCTGGTCTGG ACATCACTCA CATCTGCTTG CCTCCAGACA GCGGAGAGGA TGAGATCATA 
GATGAAATCC TGAAGATTAA TGAAGACCCC AGAGTGCATG GCCTTACCCT TCAGATCTCT 
GAAGATTCAT TGAGCAACAA AGTTCTCAAT GCTTTGAAAC CAGAAAAAGA TGTGGACGGG 
GTGACCGACA TCAATCTGGG GAAGCTCGTA CGAGGTGACG CTCCGGAGTG TTTTGTTTCA 
CCCTTGGCCA AAGCTGCTGT AGAACTTGTT GAAAAGTCAG GGATCACTTT GGATGGAAAG 
AAGGTCTTGG TCGTAGGGGC GCACGGGCCC CTGGAAGCTG CCCTGCAGTG GCTTTTCCAG 
AGAAAAGGAT CTATGACAAT GAGCTGCCCC TGGGCAACAC CTCAGCTTCC AGACAAGCTT 
CGAGAGGCAG ACATTGTGGT CTTAGGCTCA CCTAAGCCAG AAGAGATTCC TGCTGTGTGG 
ATTCCATCAG GAACCACCAT TCTCAATTGC TTCCATGACT TCTTGTCAGG GAAGCTGAGC 
GGAGGTTCTC CTGGGGTCCC CGTGGACAAA CTCATTGCAG AAGAGAGCGT GAGTCTCCTT 
GCTGCGGCTC TGCGCATTCA GAACATGGTG AGCAGCGGCA GGAGGTGGCT CAGAGAGCAG 
CAACATCGAA GATGGCGACT GCACTGCTTG AAACTGCAGC CTCTCTCACC TGTGCCCAGT 
GATATTGAGA TTTCTCGAGG ACAGACTCCA AAAGCTGTGG ATGTCCTTGC CAAGGAGATA 
GGATTGCTTG CAGATGAAAT TGAAATCTAT GGCAAGAGCA AAGCCAAAGT CCAATTGTCC 
CTGCTGGAGA GGTTAAAGGA TCAAACAGAT GGAAAATACG TCTTGGTTGC TGGGATCACG 
CCCACCCCGC TCGGAGAAGG GAAGAGTACA GTCACCATCG GACTAGTGCA GGCGCTGACC 
GCTCACCTGA AAGTCAACTC CTTCGCCTGT CTGAGGCAGC CTTCTCAGGG ACCCACTTTT 
GGAGTGAAAG GAGGAGCTGC AGGAGGTGGA TATGCTCAGG TCATCCCCAT GGAGGAGTTC 
AACCTTCACC TGACTGGGGA CATCCATGCC ATCACTGCCG CTAATAACTT GCTGGCCGCA 
GCCATCGACA CGAGAATTTT ACATGAGAGC ACTCAAACAG ACAAGGCTCT CTACAATCGA 
CTGGTTCCCT TAGTGAATGG TGTCAGAGAA TTTTCAGAAA TTCAACTTTC TCGGCTGAAG 
AAACTGGGGA TACATAAGAC TGATCCAAGC ACACTGACCG AGGAGGAAGT GAGGAAGTTC 
GCCCGCCTCA ACATTGACCC CGCAACCATC ACGTGGCAGA GAGTATTGGA CACGAACGAC 
CGATTTCTGA GAAAAATAAC CATTGGACAG GGAAGTACAG AGAAAGGATA TTCCCGGCAG 
GCACAGTTTG ACATTGCAGT GGCCAGCGAG ATCATGGCCG TGCTGGCCTT GACTGATAGC 
CTCACAGACA TGAAAGAGCG GCTAGGAAGA ATGGTGGTGG CCAGTGACAA AGACGGGCAG 
CCTGTGACAG CTGAGGACTT GGGGGTGACC GGCGCTCTGA CAGTTTTGAT GAAAGACGCC 
ATCAAGCCAA ATCTGATGCA GACCCTTGAA GGAACACCTG TGTTTGTGCA CGCTGGTCCC 
TTTGCCAACA TCGCTCACGG CAACTCATCA GTATTAGCTG ATAAAATCGC GCTGAAATTG 
GTCGGCGAAG AAGGGTTTGT GGTGACTGAA GCTGGCTTTG GGGCTGATAT TGGGATGGAG 
AAATTCTTCA ATATCAAATG CCGAGCTTCT GGCCTGGTGC CCAACGTTGT CGTGCTGGTA 
GCCACCGTTC GAGCTCTGAA GATGCATGGA GGCGGGCCGA GTGTAACTGC TGGGGTTCCT 
CTCAAGAAAG AATATACAGA GGAGAACATT CAGCTGGTAG CTGATGGCTG CTGCAACCTA 
CAGAAGCAAA TTCAGATCGC TCAGCTCTTC GGGGTGCCGG TGGTGGTGGC ACTGAATGTC 
TTCAAGACGG ACACCCGTGC AGAGATTGAC TTGGTGTGTG AGCTCGCTAA ACGGGCCGGT 
GCCTTTGATG CGGTCCCCTG CTATCACTGG TCGGCTGGAG GGAAAGGATC TGTGGACTTG 
GCCCGGGCTG TGAGAGAAGC TGCGAATAAA AGAAGCCGCT TCCAGTTCCT GTATGATGTT 
CAGCTTCCCA TCGTGGAAAA GATAAGAGTC ATTGCCCAGA CTGTCTATGG AGCCAAGGAC 
ATTGAGCTGT CTCCTGAGGC GCAGTCCAAA ATCGATCGCT ATACGCAACA GGGTTTTGGA 
AATTTGCCCA TTTGCATGGC GAAGACCCAC CTCTCTCTCT CTCATGAACC TGACAAAAAG 
GGAGTGCCGA GGGATTTCAC TCTGCCCATC AGTGATGTCC GGGCCAGCAT CGGGGCTGGG 
TTCATTTATC CTTTGGTGGG AACGATGAGC ACAATGCCAG GACTGCCCAC CCGACCCTGC 
TTTTATGATA TAGACCTTGA CACAGAAACG GAGCAAGTTA AAGGCTTGTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164257.1
CDS	181..3114
Translation	MSVRLPLLLRQLGRQQLPSGPACRLRELCRSGSRSSSSGGGDPEGLRGRRLQDGQTFSSHGPGNPEAPGMDSIVRDVIHNSKEVLSLLQEKNPAFKPVLVVIQAGDDNLMKDMNQNLAKEAGLDITHICLPPDSGEDEIIDEILKINEDPRVHGLTLQISEDSLSNKVLNALKPEKDVDGVTDINLGKLVRGDAPECFVSPLAKAAVELVEKSGITLDGKKVLVVGAHGPLEAALQWLFQRKGSMTMSCPWATPQLPDKLREADIVVLGSPKPEEIPAVWIPSGTTILNCFHDFLSGKLSGGSPGVPVDKLIAEESVSLLAAALRIQNMVSSGRRWLREQQHRRWRLHCLKLQPLSPVPSDIEISRGQTPKAVDVLAKEIGLLADEIEIYGKSKAKVQLSLLERLKDQTDGKYVLVAGITPTPLGEGKSTVTIGLVQALTAHLKVNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLLAAAIDTRILHESTQTDKALYNRLVPLVNGVREFSEIQLSRLKKLGIHKTDPSTLTEEEVRKFARLNIDPATITWQRVLDTNDRFLRKITIGQGSTEKGYSRQAQFDIAVASEIMAVLALTDSLTDMKERLGRMVVASDKDGQPVTAEDLGVTGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSVLADKIALKLVGEEGFVVTEAGFGADIGMEKFFNIKCRASGLVPNVVVLVATVRALKMHGGGPSVTAGVPLKKEYTEENIQLVADGCCNLQKQIQIAQLFGVPVVVALNVFKTDTRAEIDLVCELAKRAGAFDAVPCYHWSAGGKGSVDLARAVREAANKRSRFQFLYDVQLPIVEKIRVIAQTVYGAKDIELSPEAQSKIDRYTQQGFGNLPICMAKTHLSLSHEPDKKGVPRDFTLPISDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDTETEQVKGLF

Target ORF information:

RefSeq Version	NM_001170786.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170786.1

ORF Insert Sequence:

ATGAGCGTCC GCCTCCCTCT CCTGCTTCGC CAGCTCGGCC GCCAGCAGCT GCCCTCGGGC 
CCGGCGTGTC GCCTCCGTGA GCTGTGTCGC TCAGGCAGCA GAAGCAGCAG CAGCGGAGGT 
GGTGACCCCG AGGGGTTACG CGGGCGGCGG CTGCAGGATG GCCAAACCTT CAGCAGCCAT 
GGCCCAGGCA ATCCGGAAGC CCCCGGGATG GACTCCATCG TCAGAGACGT CATTCACAAT 
TCCAAGGAAG TGCTGAGCTT GTTGCAAGAA AAGAACCCTG CCTTTAAGCC AGTGCTTGTG 
GTTATTCAGG CCGGTGATGA CAATTTGATG AAGGATATGA ACCAGAATTT GGCCAAGGAG 
GCTGGTCTGG ACATCACTCA CATCTGCTTG CCTCCAGACA GCGGAGAGGA TGAGATCATA 
GATGAAATCC TGAAGATTAA TGAAGACCCC AGAGTGCATG GCCTTACCCT TCAGATCTCT 
GAAGATTCAT TGAGCAACAA AGTTCTCAAT GCTTTGAAAC CAGAAAAAGA TGTGGACGGG 
GTGACCGACA TCAATCTGGG GAAGCTCGTA CGAGGTGACG CTCCGGAGTG TTTTGTTTCA 
CCCTTGGCCA AAGCTGCTGT AGAACTTGTT GAAAAGTCAG GGATCACTTT GGATGGAAAG 
AAGGTCTTGG TCGTAGGGGC GCACGGGCCC CTGGAAGCTG CCCTGCAGTG GCTTTTCCAG 
AGAAAAGGAT CTATGACAAT GAGCTGCCCC TGGGCAACAC CTCAGCTTCC AGACAAGCTT 
CGAGAGGCAG ACATTGTGGT CTTAGGCTCA CCTAAGCCAG AAGAGATTCC TGCTGTGTGG 
ATTCCATCAG GAACCACCAT TCTCAATTGC TTCCATGACT TCTTGTCAGG GAAGCTGAGC 
GGAGGTTCTC CTGGGGTCCC CGTGGACAAA CTCATTGCAG AAGAGAGCGT GAGTCTCCTT 
GCTGCGGCTC TGCGCATTCA GAACATGGTG AGCAGCGGCA GGAGGTGGCT CAGAGAGCAG 
CAACATCGAA GATGGCGACT GCACTGCTTG AAACTGCAGC CTCTCTCACC TGTGCCCAGT 
GATATTGAGA TTTCTCGAGG ACAGACTCCA AAAGCTGTGG ATGTCCTTGC CAAGGAGATA 
GGATTGCTTG CAGATGAAAT TGAAATCTAT GGCAAGAGCA AAGCCAAAGT CCAATTGTCC 
CTGCTGGAGA GGTTAAAGGA TCAAACAGAT GGAAAATACG TCTTGGTTGC TGGGATCACG 
CCCACCCCGC TCGGAGAAGG GAAGAGTACA GTCACCATCG GACTAGTGCA GGCGCTGACC 
GCTCACCTGA AAGTCAACTC CTTCGCCTGT CTGAGGCAGC CTTCTCAGGG ACCCACTTTT 
GGAGTGAAAG GAGGAGCTGC AGGAGGTGGA TATGCTCAGG TCATCCCCAT GGAGGAGTTC 
AACCTTCACC TGACTGGGGA CATCCATGCC ATCACTGCCG CTAATAACTT GCTGGCCGCA 
GCCATCGACA CGAGAATTTT ACATGAGAGC ACTCAAACAG ACAAGGCTCT CTACAATCGA 
CTGGTTCCCT TAGTGAATGG TGTCAGAGAA TTTTCAGAAA TTCAACTTTC TCGGCTGAAG 
AAACTGGGGA TACATAAGAC TGATCCAAGC ACACTGACCG AGGAGGAAGT GAGGAAGTTC 
GCCCGCCTCA ACATTGACCC CGCAACCATC ACGTGGCAGA GAGTATTGGA CACGAACGAC 
CGATTTCTGA GAAAAATAAC CATTGGACAG GGAAGTACAG AGAAAGGATA TTCCCGGCAG 
GCACAGTTTG ACATTGCAGT GGCCAGCGAG ATCATGGCCG TGCTGGCCTT GACTGATAGC 
CTCACAGACA TGAAAGAGCG GCTAGGAAGA ATGGTGGTGG CCAGTGACAA AGACGGGCAG 
CCTGTGACAG CTGAGGACTT GGGGGTGACC GGCGCTCTGA CAGTTTTGAT GAAAGACGCC 
ATCAAGCCAA ATCTGATGCA GACCCTTGAA GGAACACCTG TGTTTGTGCA CGCTGGTCCC 
TTTGCCAACA TCGCTCACGG CAACTCATCA GTATTAGCTG ATAAAATCGC GCTGAAATTG 
GTCGGCGAAG AAGGGTTTGT GGTGACTGAA GCTGGCTTTG GGGCTGATAT TGGGATGGAG 
AAATTCTTCA ATATCAAATG CCGAGCTTCT GGCCTGGTGC CCAACGTTGT CGTGCTGGTA 
GCCACCGTTC GAGCTCTGAA GATGCATGGA GGCGGGCCGA GTGTAACTGC TGGGGTTCCT 
CTCAAGAAAG AATATACAGA GGAGAACATT CAGCTGGTAG CTGATGGCTG CTGCAACCTA 
CAGAAGCAAA TTCAGATCGC TCAGCTCTTC GGGGTGCCGG TGGTGGTGGC ACTGAATGTC 
TTCAAGACGG ACACCCGTGC AGAGATTGAC TTGGTGTGTG AGCTCGCTAA ACGGGCCGGT 
GCCTTTGATG CGGTCCCCTG CTATCACTGG TCGGCTGGAG GGAAAGGATC TGTGGACTTG 
GCCCGGGCTG TGAGAGAAGC TGCGAATAAA AGAAGCCGCT TCCAGTTCCT GTATGATGTT 
CAGCTTCCCA TCGTGGAAAA GATAAGAGTC ATTGCCCAGA CTGTCTATGG AGCCAAGGAC 
ATTGAGCTGT CTCCTGAGGC GCAGTCCAAA ATCGATCGCT ATACGCAACA GGGTTTTGGA 
AATTTGCCCA TTTGCATGGC GAAGACCCAC CTCTCTCTCT CTCATGAACC TGACAAAAAG 
GGAGTGCCGA GGGATTTCAC TCTGCCCATC AGTGATGTCC GGGCCAGCAT CGGGGCTGGG 
TTCATTTATC CTTTGGTGGG AACGATGAGC ACAATGCCAG GACTGCCCAC CCGACCCTGC 
TTTTATGATA TAGACCTTGA CACAGAAACG GAGCAAGTTA AAGGCTTGTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mthfd1l ( NM_172308.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_758512.3 Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), transcript variant 2, mRNA.

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CloneID	OMu15583
Clone ID Related Accession (Same CDS sequence)	NM_001170785.1 , NM_001170786.1 , NM_172308.4
Accession Version	NM_172308.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	monofunctional C1-tetrahydrofolate synthase, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC153570.9, AK028211.1 and AC159148.10. On Jan 6, 2010 this sequence version replaced NM_172308.3. Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK038579.1, SRR1660813.4710.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCGTCC GCCTCCCTCT CCTGCTTCGC CAGCTCGGCC GCCAGCAGCT GCCCTCGGGC 
CCGGCGTGTC GCCTCCGTGA GCTGTGTCGC TCAGGCAGCA GAAGCAGCAG CAGCGGAGGT 
GGTGACCCCG AGGGGTTACG CGGGCGGCGG CTGCAGGATG GCCAAACCTT CAGCAGCCAT 
GGCCCAGGCA ATCCGGAAGC CCCCGGGATG GACTCCATCG TCAGAGACGT CATTCACAAT 
TCCAAGGAAG TGCTGAGCTT GTTGCAAGAA AAGAACCCTG CCTTTAAGCC AGTGCTTGTG 
GTTATTCAGG CCGGTGATGA CAATTTGATG AAGGATATGA ACCAGAATTT GGCCAAGGAG 
GCTGGTCTGG ACATCACTCA CATCTGCTTG CCTCCAGACA GCGGAGAGGA TGAGATCATA 
GATGAAATCC TGAAGATTAA TGAAGACCCC AGAGTGCATG GCCTTACCCT TCAGATCTCT 
GAAGATTCAT TGAGCAACAA AGTTCTCAAT GCTTTGAAAC CAGAAAAAGA TGTGGACGGG 
GTGACCGACA TCAATCTGGG GAAGCTCGTA CGAGGTGACG CTCCGGAGTG TTTTGTTTCA 
CCCTTGGCCA AAGCTGCTGT AGAACTTGTT GAAAAGTCAG GGATCACTTT GGATGGAAAG 
AAGGTCTTGG TCGTAGGGGC GCACGGGCCC CTGGAAGCTG CCCTGCAGTG GCTTTTCCAG 
AGAAAAGGAT CTATGACAAT GAGCTGCCCC TGGGCAACAC CTCAGCTTCC AGACAAGCTT 
CGAGAGGCAG ACATTGTGGT CTTAGGCTCA CCTAAGCCAG AAGAGATTCC TGCTGTGTGG 
ATTCCATCAG GAACCACCAT TCTCAATTGC TTCCATGACT TCTTGTCAGG GAAGCTGAGC 
GGAGGTTCTC CTGGGGTCCC CGTGGACAAA CTCATTGCAG AAGAGAGCGT GAGTCTCCTT 
GCTGCGGCTC TGCGCATTCA GAACATGGTG AGCAGCGGCA GGAGGTGGCT CAGAGAGCAG 
CAACATCGAA GATGGCGACT GCACTGCTTG AAACTGCAGC CTCTCTCACC TGTGCCCAGT 
GATATTGAGA TTTCTCGAGG ACAGACTCCA AAAGCTGTGG ATGTCCTTGC CAAGGAGATA 
GGATTGCTTG CAGATGAAAT TGAAATCTAT GGCAAGAGCA AAGCCAAAGT CCAATTGTCC 
CTGCTGGAGA GGTTAAAGGA TCAAACAGAT GGAAAATACG TCTTGGTTGC TGGGATCACG 
CCCACCCCGC TCGGAGAAGG GAAGAGTACA GTCACCATCG GACTAGTGCA GGCGCTGACC 
GCTCACCTGA AAGTCAACTC CTTCGCCTGT CTGAGGCAGC CTTCTCAGGG ACCCACTTTT 
GGAGTGAAAG GAGGAGCTGC AGGAGGTGGA TATGCTCAGG TCATCCCCAT GGAGGAGTTC 
AACCTTCACC TGACTGGGGA CATCCATGCC ATCACTGCCG CTAATAACTT GCTGGCCGCA 
GCCATCGACA CGAGAATTTT ACATGAGAGC ACTCAAACAG ACAAGGCTCT CTACAATCGA 
CTGGTTCCCT TAGTGAATGG TGTCAGAGAA TTTTCAGAAA TTCAACTTTC TCGGCTGAAG 
AAACTGGGGA TACATAAGAC TGATCCAAGC ACACTGACCG AGGAGGAAGT GAGGAAGTTC 
GCCCGCCTCA ACATTGACCC CGCAACCATC ACGTGGCAGA GAGTATTGGA CACGAACGAC 
CGATTTCTGA GAAAAATAAC CATTGGACAG GGAAGTACAG AGAAAGGATA TTCCCGGCAG 
GCACAGTTTG ACATTGCAGT GGCCAGCGAG ATCATGGCCG TGCTGGCCTT GACTGATAGC 
CTCACAGACA TGAAAGAGCG GCTAGGAAGA ATGGTGGTGG CCAGTGACAA AGACGGGCAG 
CCTGTGACAG CTGAGGACTT GGGGGTGACC GGCGCTCTGA CAGTTTTGAT GAAAGACGCC 
ATCAAGCCAA ATCTGATGCA GACCCTTGAA GGAACACCTG TGTTTGTGCA CGCTGGTCCC 
TTTGCCAACA TCGCTCACGG CAACTCATCA GTATTAGCTG ATAAAATCGC GCTGAAATTG 
GTCGGCGAAG AAGGGTTTGT GGTGACTGAA GCTGGCTTTG GGGCTGATAT TGGGATGGAG 
AAATTCTTCA ATATCAAATG CCGAGCTTCT GGCCTGGTGC CCAACGTTGT CGTGCTGGTA 
GCCACCGTTC GAGCTCTGAA GATGCATGGA GGCGGGCCGA GTGTAACTGC TGGGGTTCCT 
CTCAAGAAAG AATATACAGA GGAGAACATT CAGCTGGTAG CTGATGGCTG CTGCAACCTA 
CAGAAGCAAA TTCAGATCGC TCAGCTCTTC GGGGTGCCGG TGGTGGTGGC ACTGAATGTC 
TTCAAGACGG ACACCCGTGC AGAGATTGAC TTGGTGTGTG AGCTCGCTAA ACGGGCCGGT 
GCCTTTGATG CGGTCCCCTG CTATCACTGG TCGGCTGGAG GGAAAGGATC TGTGGACTTG 
GCCCGGGCTG TGAGAGAAGC TGCGAATAAA AGAAGCCGCT TCCAGTTCCT GTATGATGTT 
CAGCTTCCCA TCGTGGAAAA GATAAGAGTC ATTGCCCAGA CTGTCTATGG AGCCAAGGAC 
ATTGAGCTGT CTCCTGAGGC GCAGTCCAAA ATCGATCGCT ATACGCAACA GGGTTTTGGA 
AATTTGCCCA TTTGCATGGC GAAGACCCAC CTCTCTCTCT CTCATGAACC TGACAAAAAG 
GGAGTGCCGA GGGATTTCAC TCTGCCCATC AGTGATGTCC GGGCCAGCAT CGGGGCTGGG 
TTCATTTATC CTTTGGTGGG AACGATGAGC ACAATGCCAG GACTGCCCAC CCGACCCTGC 
TTTTATGATA TAGACCTTGA CACAGAAACG GAGCAAGTTA AAGGCTTGTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_758512.3
CDS	181..3114
Translation	MSVRLPLLLRQLGRQQLPSGPACRLRELCRSGSRSSSSGGGDPEGLRGRRLQDGQTFSSHGPGNPEAPGMDSIVRDVIHNSKEVLSLLQEKNPAFKPVLVVIQAGDDNLMKDMNQNLAKEAGLDITHICLPPDSGEDEIIDEILKINEDPRVHGLTLQISEDSLSNKVLNALKPEKDVDGVTDINLGKLVRGDAPECFVSPLAKAAVELVEKSGITLDGKKVLVVGAHGPLEAALQWLFQRKGSMTMSCPWATPQLPDKLREADIVVLGSPKPEEIPAVWIPSGTTILNCFHDFLSGKLSGGSPGVPVDKLIAEESVSLLAAALRIQNMVSSGRRWLREQQHRRWRLHCLKLQPLSPVPSDIEISRGQTPKAVDVLAKEIGLLADEIEIYGKSKAKVQLSLLERLKDQTDGKYVLVAGITPTPLGEGKSTVTIGLVQALTAHLKVNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLLAAAIDTRILHESTQTDKALYNRLVPLVNGVREFSEIQLSRLKKLGIHKTDPSTLTEEEVRKFARLNIDPATITWQRVLDTNDRFLRKITIGQGSTEKGYSRQAQFDIAVASEIMAVLALTDSLTDMKERLGRMVVASDKDGQPVTAEDLGVTGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSVLADKIALKLVGEEGFVVTEAGFGADIGMEKFFNIKCRASGLVPNVVVLVATVRALKMHGGGPSVTAGVPLKKEYTEENIQLVADGCCNLQKQIQIAQLFGVPVVVALNVFKTDTRAEIDLVCELAKRAGAFDAVPCYHWSAGGKGSVDLARAVREAANKRSRFQFLYDVQLPIVEKIRVIAQTVYGAKDIELSPEAQSKIDRYTQQGFGNLPICMAKTHLSLSHEPDKKGVPRDFTLPISDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDTETEQVKGLF

Target ORF information:

RefSeq Version	NM_172308.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172308.4

ORF Insert Sequence:

ATGAGCGTCC GCCTCCCTCT CCTGCTTCGC CAGCTCGGCC GCCAGCAGCT GCCCTCGGGC 
CCGGCGTGTC GCCTCCGTGA GCTGTGTCGC TCAGGCAGCA GAAGCAGCAG CAGCGGAGGT 
GGTGACCCCG AGGGGTTACG CGGGCGGCGG CTGCAGGATG GCCAAACCTT CAGCAGCCAT 
GGCCCAGGCA ATCCGGAAGC CCCCGGGATG GACTCCATCG TCAGAGACGT CATTCACAAT 
TCCAAGGAAG TGCTGAGCTT GTTGCAAGAA AAGAACCCTG CCTTTAAGCC AGTGCTTGTG 
GTTATTCAGG CCGGTGATGA CAATTTGATG AAGGATATGA ACCAGAATTT GGCCAAGGAG 
GCTGGTCTGG ACATCACTCA CATCTGCTTG CCTCCAGACA GCGGAGAGGA TGAGATCATA 
GATGAAATCC TGAAGATTAA TGAAGACCCC AGAGTGCATG GCCTTACCCT TCAGATCTCT 
GAAGATTCAT TGAGCAACAA AGTTCTCAAT GCTTTGAAAC CAGAAAAAGA TGTGGACGGG 
GTGACCGACA TCAATCTGGG GAAGCTCGTA CGAGGTGACG CTCCGGAGTG TTTTGTTTCA 
CCCTTGGCCA AAGCTGCTGT AGAACTTGTT GAAAAGTCAG GGATCACTTT GGATGGAAAG 
AAGGTCTTGG TCGTAGGGGC GCACGGGCCC CTGGAAGCTG CCCTGCAGTG GCTTTTCCAG 
AGAAAAGGAT CTATGACAAT GAGCTGCCCC TGGGCAACAC CTCAGCTTCC AGACAAGCTT 
CGAGAGGCAG ACATTGTGGT CTTAGGCTCA CCTAAGCCAG AAGAGATTCC TGCTGTGTGG 
ATTCCATCAG GAACCACCAT TCTCAATTGC TTCCATGACT TCTTGTCAGG GAAGCTGAGC 
GGAGGTTCTC CTGGGGTCCC CGTGGACAAA CTCATTGCAG AAGAGAGCGT GAGTCTCCTT 
GCTGCGGCTC TGCGCATTCA GAACATGGTG AGCAGCGGCA GGAGGTGGCT CAGAGAGCAG 
CAACATCGAA GATGGCGACT GCACTGCTTG AAACTGCAGC CTCTCTCACC TGTGCCCAGT 
GATATTGAGA TTTCTCGAGG ACAGACTCCA AAAGCTGTGG ATGTCCTTGC CAAGGAGATA 
GGATTGCTTG CAGATGAAAT TGAAATCTAT GGCAAGAGCA AAGCCAAAGT CCAATTGTCC 
CTGCTGGAGA GGTTAAAGGA TCAAACAGAT GGAAAATACG TCTTGGTTGC TGGGATCACG 
CCCACCCCGC TCGGAGAAGG GAAGAGTACA GTCACCATCG GACTAGTGCA GGCGCTGACC 
GCTCACCTGA AAGTCAACTC CTTCGCCTGT CTGAGGCAGC CTTCTCAGGG ACCCACTTTT 
GGAGTGAAAG GAGGAGCTGC AGGAGGTGGA TATGCTCAGG TCATCCCCAT GGAGGAGTTC 
AACCTTCACC TGACTGGGGA CATCCATGCC ATCACTGCCG CTAATAACTT GCTGGCCGCA 
GCCATCGACA CGAGAATTTT ACATGAGAGC ACTCAAACAG ACAAGGCTCT CTACAATCGA 
CTGGTTCCCT TAGTGAATGG TGTCAGAGAA TTTTCAGAAA TTCAACTTTC TCGGCTGAAG 
AAACTGGGGA TACATAAGAC TGATCCAAGC ACACTGACCG AGGAGGAAGT GAGGAAGTTC 
GCCCGCCTCA ACATTGACCC CGCAACCATC ACGTGGCAGA GAGTATTGGA CACGAACGAC 
CGATTTCTGA GAAAAATAAC CATTGGACAG GGAAGTACAG AGAAAGGATA TTCCCGGCAG 
GCACAGTTTG ACATTGCAGT GGCCAGCGAG ATCATGGCCG TGCTGGCCTT GACTGATAGC 
CTCACAGACA TGAAAGAGCG GCTAGGAAGA ATGGTGGTGG CCAGTGACAA AGACGGGCAG 
CCTGTGACAG CTGAGGACTT GGGGGTGACC GGCGCTCTGA CAGTTTTGAT GAAAGACGCC 
ATCAAGCCAA ATCTGATGCA GACCCTTGAA GGAACACCTG TGTTTGTGCA CGCTGGTCCC 
TTTGCCAACA TCGCTCACGG CAACTCATCA GTATTAGCTG ATAAAATCGC GCTGAAATTG 
GTCGGCGAAG AAGGGTTTGT GGTGACTGAA GCTGGCTTTG GGGCTGATAT TGGGATGGAG 
AAATTCTTCA ATATCAAATG CCGAGCTTCT GGCCTGGTGC CCAACGTTGT CGTGCTGGTA 
GCCACCGTTC GAGCTCTGAA GATGCATGGA GGCGGGCCGA GTGTAACTGC TGGGGTTCCT 
CTCAAGAAAG AATATACAGA GGAGAACATT CAGCTGGTAG CTGATGGCTG CTGCAACCTA 
CAGAAGCAAA TTCAGATCGC TCAGCTCTTC GGGGTGCCGG TGGTGGTGGC ACTGAATGTC 
TTCAAGACGG ACACCCGTGC AGAGATTGAC TTGGTGTGTG AGCTCGCTAA ACGGGCCGGT 
GCCTTTGATG CGGTCCCCTG CTATCACTGG TCGGCTGGAG GGAAAGGATC TGTGGACTTG 
GCCCGGGCTG TGAGAGAAGC TGCGAATAAA AGAAGCCGCT TCCAGTTCCT GTATGATGTT 
CAGCTTCCCA TCGTGGAAAA GATAAGAGTC ATTGCCCAGA CTGTCTATGG AGCCAAGGAC 
ATTGAGCTGT CTCCTGAGGC GCAGTCCAAA ATCGATCGCT ATACGCAACA GGGTTTTGGA 
AATTTGCCCA TTTGCATGGC GAAGACCCAC CTCTCTCTCT CTCATGAACC TGACAAAAAG 
GGAGTGCCGA GGGATTTCAC TCTGCCCATC AGTGATGTCC GGGCCAGCAT CGGGGCTGGG 
TTCATTTATC CTTTGGTGGG AACGATGAGC ACAATGCCAG GACTGCCCAC CCGACCCTGC 
TTTTATGATA TAGACCTTGA CACAGAAACG GAGCAAGTTA AAGGCTTGTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mthfd1l ( XM_006512449.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512512.1 Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), transcript variant X1, mRNA.

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CloneID	OMu43654
Clone ID Related Accession (Same CDS sequence)	XM_006512449.3 , XM_006512449.4
Accession Version	XM_006512449.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512449.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTCC GCCTCCCTCT CCTGCTTCGC CAGCTCGGCC GCCAGCAGCT GCCCTCGGGC 
CCGGCGTGTC GCCTCCGTGA GCTGTGTCGC TCAGGCAGCA GAAGCAGCAG CAGCGGAGGT 
GGTGACCCCG AGGGGTTACG CGGGCGGCGG CTGCAGGATG GCCAAACCTT CAGCAGCCAT 
GGCCCAGGCA ATCCGGAAGC CCCCGGGATG GACTCCATCG TCAGAGACGT CATTCACAAT 
TCCAAGGAAG TGCTGAGCTT GTTGCAAGAA AAGAACCCTG CCTTTAAGCC AGTGCTTGTG 
GTTATTCAGG CCGGTGATGA CAATTTGATG AAGGATATGA ACCAGAATTT GGCCAAGGAG 
GCTGGTCTGG ACATCACTCA CATCTGCTTG CCTCCAGACA GCGGAGAGGA TGAGATCATA 
GATGAAATCC TGAAGATTAA TGAAGACCCC AGAGTGCATG GCCTTACCCT TCAGATCTCT 
GAAGATTCAT TGAGCAACAA AGTTCTCAAT GCTTTGAAAC CAGAAAAAGA TGTGGACGGG 
GTGACCGACA TCAATCTGGG GAAGCTCGTA CGAGGTGACG CTCCGGAGTG TTTTGTTTCA 
CCCTTGGCCA AAGCTGCTGT AGAACTTGTT GAAAAGTCAG GGATCACTTT GGATGGAAAG 
AAGGTCTTGG TCGTAGGGGC GCACGGGCCC CTGGAAGCTG CCCTGCAGTG GCTTTTCCAG 
AGAAAAGGAT CTATGACAAT GAGCTGCCCC TGGGCAACAC CTCAGCTTCC AGACAAGCTT 
CGAGAGGCAG ACATTGTGGT CTTAGGCTCA CCTAAGCCAG AAGAGATTCC TGCTGTGTGG 
ATTCCATCAG GAACCACCAT TCTCAATTGC TTCCATGACT TCTTGTCAGG GAAGCTGAGC 
GGAGGTTCTC CTGGGGTCCC CGTGGACAAA CTCATTGCAG AAGAGAGCGT GAGTCTCCTT 
GCTGCGGCTC TGCGCATTCA GAACATGGTG AGCAGCGGCA GGAGGTGGCT CAGAGAGCAG 
CAACATCGAA GATGGCGACT GCACTGCTTG AAACTGCAGC CTCTCTCACC TGTGCCCAGT 
GATATTGAGA TTTCTCGAGG ACAGACTCCA AAAGCTGTGG ATGTCCTTGC CAAGGAGATA 
GGATTGCTTG CAGATGAAAT TGAAATCTAT GGCAAGAGCA AAGCCAAAGT CCAATTGTCC 
CTGCTGGAGA GGTTAAAGGA TCAAACAGAT GGAAAATACG TCTTGGTTGC TGGGATCACG 
CCCACCCCGC TCGGAGAAGG GAAGAGTACA GTCACCATCG GACTAGTGCA GGCGCTGACC 
GCTCACCTGA AAGTCAACTC CTTCGCCTGT CTGAGGCAGC CTTCTCAGGG ACCCACTTTT 
GGAGTGAAAG GAGGAGCTGC AGGAGGTGGA TATGCTCAGG TCATCCCCAT GGAGGAGTTC 
AACCTTCACC TGACTGGGGA CATCCATGCC ATCACTGCCG CTAATAACTT GCTGGCCGCA 
GCCATCGACA CGAGAATTTT ACATGAGAGC ACTCAAACAG ACAAGGCTCT CTACAATCGA 
CTGGTTCCCT TAGTGAATGG TGTCAGAGAA TTTTCAGAAA TTCAACTTTC TCGGCTGAAG 
AAACTGGGGA TACATAAGAC TGATCCAAGC ACACTGACCG AGGAGGAAGT GAGGAAGTTC 
GCCCGCCTCA ACATTGACCC CGCAACCATC ACGTGGCAGA GAGTATTGGA CACGAACGAC 
CGATTTCTGA GAAAAATAAC CATTGGACAG GGAAGTACAG AGAAAGGATA TTCCCGGCAG 
GCACAGTTTG ACATTGCAGT GGCCAGCGAG ATCATGGCCG TGCTGGCCTT GACTGATAGC 
CTCACAGACA TGAAAGAGCG GCTAGGAAGA ATGGTGGTGG CCAGTGACAA AGACGGGCAG 
CCTGTGACAG CTGAGGACTT GGGGGTGACC GGCGCTCTGA CAGTTTTGAT GAAAGACGCC 
ATCAAGCCAA ATCTGATGCA GACCCTTGAA GGAACACCTG TGTTTGTGCA CGCTGGTCCC 
TTTGCCAACA TCGCTCACGG CAACTCATCA GTATTAGCTG ATAAAATCGC GCTGAAATTG 
GTCGGCGAAG AAGGGTTTGT GGAGTACACC CAGAATAGTT TCTCCATGCT CTCTGCATCA 
ACCCCAACCT TCCCCAGTGT GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512512.1
CDS	474..2660
Translation	MSVRLPLLLRQLGRQQLPSGPACRLRELCRSGSRSSSSGGGDPEGLRGRRLQDGQTFSSHGPGNPEAPGMDSIVRDVIHNSKEVLSLLQEKNPAFKPVLVVIQAGDDNLMKDMNQNLAKEAGLDITHICLPPDSGEDEIIDEILKINEDPRVHGLTLQISEDSLSNKVLNALKPEKDVDGVTDINLGKLVRGDAPECFVSPLAKAAVELVEKSGITLDGKKVLVVGAHGPLEAALQWLFQRKGSMTMSCPWATPQLPDKLREADIVVLGSPKPEEIPAVWIPSGTTILNCFHDFLSGKLSGGSPGVPVDKLIAEESVSLLAAALRIQNMVSSGRRWLREQQHRRWRLHCLKLQPLSPVPSDIEISRGQTPKAVDVLAKEIGLLADEIEIYGKSKAKVQLSLLERLKDQTDGKYVLVAGITPTPLGEGKSTVTIGLVQALTAHLKVNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLLAAAIDTRILHESTQTDKALYNRLVPLVNGVREFSEIQLSRLKKLGIHKTDPSTLTEEEVRKFARLNIDPATITWQRVLDTNDRFLRKITIGQGSTEKGYSRQAQFDIAVASEIMAVLALTDSLTDMKERLGRMVVASDKDGQPVTAEDLGVTGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSVLADKIALKLVGEEGFVEYTQNSFSMLSASTPTFPSVW

Target ORF information:

RefSeq Version	XM_006512449.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512449.3

ORF Insert Sequence:

ATGAGCGTCC GCCTCCCTCT CCTGCTTCGC CAGCTCGGCC GCCAGCAGCT GCCCTCGGGC 
CCGGCGTGTC GCCTCCGTGA GCTGTGTCGC TCAGGCAGCA GAAGCAGCAG CAGCGGAGGT 
GGTGACCCCG AGGGGTTACG CGGGCGGCGG CTGCAGGATG GCCAAACCTT CAGCAGCCAT 
GGCCCAGGCA ATCCGGAAGC CCCCGGGATG GACTCCATCG TCAGAGACGT CATTCACAAT 
TCCAAGGAAG TGCTGAGCTT GTTGCAAGAA AAGAACCCTG CCTTTAAGCC AGTGCTTGTG 
GTTATTCAGG CCGGTGATGA CAATTTGATG AAGGATATGA ACCAGAATTT GGCCAAGGAG 
GCTGGTCTGG ACATCACTCA CATCTGCTTG CCTCCAGACA GCGGAGAGGA TGAGATCATA 
GATGAAATCC TGAAGATTAA TGAAGACCCC AGAGTGCATG GCCTTACCCT TCAGATCTCT 
GAAGATTCAT TGAGCAACAA AGTTCTCAAT GCTTTGAAAC CAGAAAAAGA TGTGGACGGG 
GTGACCGACA TCAATCTGGG GAAGCTCGTA CGAGGTGACG CTCCGGAGTG TTTTGTTTCA 
CCCTTGGCCA AAGCTGCTGT AGAACTTGTT GAAAAGTCAG GGATCACTTT GGATGGAAAG 
AAGGTCTTGG TCGTAGGGGC GCACGGGCCC CTGGAAGCTG CCCTGCAGTG GCTTTTCCAG 
AGAAAAGGAT CTATGACAAT GAGCTGCCCC TGGGCAACAC CTCAGCTTCC AGACAAGCTT 
CGAGAGGCAG ACATTGTGGT CTTAGGCTCA CCTAAGCCAG AAGAGATTCC TGCTGTGTGG 
ATTCCATCAG GAACCACCAT TCTCAATTGC TTCCATGACT TCTTGTCAGG GAAGCTGAGC 
GGAGGTTCTC CTGGGGTCCC CGTGGACAAA CTCATTGCAG AAGAGAGCGT GAGTCTCCTT 
GCTGCGGCTC TGCGCATTCA GAACATGGTG AGCAGCGGCA GGAGGTGGCT CAGAGAGCAG 
CAACATCGAA GATGGCGACT GCACTGCTTG AAACTGCAGC CTCTCTCACC TGTGCCCAGT 
GATATTGAGA TTTCTCGAGG ACAGACTCCA AAAGCTGTGG ATGTCCTTGC CAAGGAGATA 
GGATTGCTTG CAGATGAAAT TGAAATCTAT GGCAAGAGCA AAGCCAAAGT CCAATTGTCC 
CTGCTGGAGA GGTTAAAGGA TCAAACAGAT GGAAAATACG TCTTGGTTGC TGGGATCACG 
CCCACCCCGC TCGGAGAAGG GAAGAGTACA GTCACCATCG GACTAGTGCA GGCGCTGACC 
GCTCACCTGA AAGTCAACTC CTTCGCCTGT CTGAGGCAGC CTTCTCAGGG ACCCACTTTT 
GGAGTGAAAG GAGGAGCTGC AGGAGGTGGA TATGCTCAGG TCATCCCCAT GGAGGAGTTC 
AACCTTCACC TGACTGGGGA CATCCATGCC ATCACTGCCG CTAATAACTT GCTGGCCGCA 
GCCATCGACA CGAGAATTTT ACATGAGAGC ACTCAAACAG ACAAGGCTCT CTACAATCGA 
CTGGTTCCCT TAGTGAATGG TGTCAGAGAA TTTTCAGAAA TTCAACTTTC TCGGCTGAAG 
AAACTGGGGA TACATAAGAC TGATCCAAGC ACACTGACCG AGGAGGAAGT GAGGAAGTTC 
GCCCGCCTCA ACATTGACCC CGCAACCATC ACGTGGCAGA GAGTATTGGA CACGAACGAC 
CGATTTCTGA GAAAAATAAC CATTGGACAG GGAAGTACAG AGAAAGGATA TTCCCGGCAG 
GCACAGTTTG ACATTGCAGT GGCCAGCGAG ATCATGGCCG TGCTGGCCTT GACTGATAGC 
CTCACAGACA TGAAAGAGCG GCTAGGAAGA ATGGTGGTGG CCAGTGACAA AGACGGGCAG 
CCTGTGACAG CTGAGGACTT GGGGGTGACC GGCGCTCTGA CAGTTTTGAT GAAAGACGCC 
ATCAAGCCAA ATCTGATGCA GACCCTTGAA GGAACACCTG TGTTTGTGCA CGCTGGTCCC 
TTTGCCAACA TCGCTCACGG CAACTCATCA GTATTAGCTG ATAAAATCGC GCTGAAATTG 
GTCGGCGAAG AAGGGTTTGT GGAGTACACC CAGAATAGTT TCTCCATGCT CTCTGCATCA 
ACCCCAACCT TCCCCAGTGT GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mthfd1l ( XM_006512449.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512512.1 Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), transcript variant X1, mRNA.

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CloneID	OMu43654
Clone ID Related Accession (Same CDS sequence)	XM_006512449.3 , XM_006512449.4
Accession Version	XM_006512449.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512449.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTCC GCCTCCCTCT CCTGCTTCGC CAGCTCGGCC GCCAGCAGCT GCCCTCGGGC 
CCGGCGTGTC GCCTCCGTGA GCTGTGTCGC TCAGGCAGCA GAAGCAGCAG CAGCGGAGGT 
GGTGACCCCG AGGGGTTACG CGGGCGGCGG CTGCAGGATG GCCAAACCTT CAGCAGCCAT 
GGCCCAGGCA ATCCGGAAGC CCCCGGGATG GACTCCATCG TCAGAGACGT CATTCACAAT 
TCCAAGGAAG TGCTGAGCTT GTTGCAAGAA AAGAACCCTG CCTTTAAGCC AGTGCTTGTG 
GTTATTCAGG CCGGTGATGA CAATTTGATG AAGGATATGA ACCAGAATTT GGCCAAGGAG 
GCTGGTCTGG ACATCACTCA CATCTGCTTG CCTCCAGACA GCGGAGAGGA TGAGATCATA 
GATGAAATCC TGAAGATTAA TGAAGACCCC AGAGTGCATG GCCTTACCCT TCAGATCTCT 
GAAGATTCAT TGAGCAACAA AGTTCTCAAT GCTTTGAAAC CAGAAAAAGA TGTGGACGGG 
GTGACCGACA TCAATCTGGG GAAGCTCGTA CGAGGTGACG CTCCGGAGTG TTTTGTTTCA 
CCCTTGGCCA AAGCTGCTGT AGAACTTGTT GAAAAGTCAG GGATCACTTT GGATGGAAAG 
AAGGTCTTGG TCGTAGGGGC GCACGGGCCC CTGGAAGCTG CCCTGCAGTG GCTTTTCCAG 
AGAAAAGGAT CTATGACAAT GAGCTGCCCC TGGGCAACAC CTCAGCTTCC AGACAAGCTT 
CGAGAGGCAG ACATTGTGGT CTTAGGCTCA CCTAAGCCAG AAGAGATTCC TGCTGTGTGG 
ATTCCATCAG GAACCACCAT TCTCAATTGC TTCCATGACT TCTTGTCAGG GAAGCTGAGC 
GGAGGTTCTC CTGGGGTCCC CGTGGACAAA CTCATTGCAG AAGAGAGCGT GAGTCTCCTT 
GCTGCGGCTC TGCGCATTCA GAACATGGTG AGCAGCGGCA GGAGGTGGCT CAGAGAGCAG 
CAACATCGAA GATGGCGACT GCACTGCTTG AAACTGCAGC CTCTCTCACC TGTGCCCAGT 
GATATTGAGA TTTCTCGAGG ACAGACTCCA AAAGCTGTGG ATGTCCTTGC CAAGGAGATA 
GGATTGCTTG CAGATGAAAT TGAAATCTAT GGCAAGAGCA AAGCCAAAGT CCAATTGTCC 
CTGCTGGAGA GGTTAAAGGA TCAAACAGAT GGAAAATACG TCTTGGTTGC TGGGATCACG 
CCCACCCCGC TCGGAGAAGG GAAGAGTACA GTCACCATCG GACTAGTGCA GGCGCTGACC 
GCTCACCTGA AAGTCAACTC CTTCGCCTGT CTGAGGCAGC CTTCTCAGGG ACCCACTTTT 
GGAGTGAAAG GAGGAGCTGC AGGAGGTGGA TATGCTCAGG TCATCCCCAT GGAGGAGTTC 
AACCTTCACC TGACTGGGGA CATCCATGCC ATCACTGCCG CTAATAACTT GCTGGCCGCA 
GCCATCGACA CGAGAATTTT ACATGAGAGC ACTCAAACAG ACAAGGCTCT CTACAATCGA 
CTGGTTCCCT TAGTGAATGG TGTCAGAGAA TTTTCAGAAA TTCAACTTTC TCGGCTGAAG 
AAACTGGGGA TACATAAGAC TGATCCAAGC ACACTGACCG AGGAGGAAGT GAGGAAGTTC 
GCCCGCCTCA ACATTGACCC CGCAACCATC ACGTGGCAGA GAGTATTGGA CACGAACGAC 
CGATTTCTGA GAAAAATAAC CATTGGACAG GGAAGTACAG AGAAAGGATA TTCCCGGCAG 
GCACAGTTTG ACATTGCAGT GGCCAGCGAG ATCATGGCCG TGCTGGCCTT GACTGATAGC 
CTCACAGACA TGAAAGAGCG GCTAGGAAGA ATGGTGGTGG CCAGTGACAA AGACGGGCAG 
CCTGTGACAG CTGAGGACTT GGGGGTGACC GGCGCTCTGA CAGTTTTGAT GAAAGACGCC 
ATCAAGCCAA ATCTGATGCA GACCCTTGAA GGAACACCTG TGTTTGTGCA CGCTGGTCCC 
TTTGCCAACA TCGCTCACGG CAACTCATCA GTATTAGCTG ATAAAATCGC GCTGAAATTG 
GTCGGCGAAG AAGGGTTTGT GGAGTACACC CAGAATAGTT TCTCCATGCT CTCTGCATCA 
ACCCCAACCT TCCCCAGTGT GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512512.1
CDS	364..2550
Translation	MSVRLPLLLRQLGRQQLPSGPACRLRELCRSGSRSSSSGGGDPEGLRGRRLQDGQTFSSHGPGNPEAPGMDSIVRDVIHNSKEVLSLLQEKNPAFKPVLVVIQAGDDNLMKDMNQNLAKEAGLDITHICLPPDSGEDEIIDEILKINEDPRVHGLTLQISEDSLSNKVLNALKPEKDVDGVTDINLGKLVRGDAPECFVSPLAKAAVELVEKSGITLDGKKVLVVGAHGPLEAALQWLFQRKGSMTMSCPWATPQLPDKLREADIVVLGSPKPEEIPAVWIPSGTTILNCFHDFLSGKLSGGSPGVPVDKLIAEESVSLLAAALRIQNMVSSGRRWLREQQHRRWRLHCLKLQPLSPVPSDIEISRGQTPKAVDVLAKEIGLLADEIEIYGKSKAKVQLSLLERLKDQTDGKYVLVAGITPTPLGEGKSTVTIGLVQALTAHLKVNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLLAAAIDTRILHESTQTDKALYNRLVPLVNGVREFSEIQLSRLKKLGIHKTDPSTLTEEEVRKFARLNIDPATITWQRVLDTNDRFLRKITIGQGSTEKGYSRQAQFDIAVASEIMAVLALTDSLTDMKERLGRMVVASDKDGQPVTAEDLGVTGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSVLADKIALKLVGEEGFVEYTQNSFSMLSASTPTFPSVW

Target ORF information:

RefSeq Version	XM_006512449.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512449.4

ORF Insert Sequence:

ATGAGCGTCC GCCTCCCTCT CCTGCTTCGC CAGCTCGGCC GCCAGCAGCT GCCCTCGGGC 
CCGGCGTGTC GCCTCCGTGA GCTGTGTCGC TCAGGCAGCA GAAGCAGCAG CAGCGGAGGT 
GGTGACCCCG AGGGGTTACG CGGGCGGCGG CTGCAGGATG GCCAAACCTT CAGCAGCCAT 
GGCCCAGGCA ATCCGGAAGC CCCCGGGATG GACTCCATCG TCAGAGACGT CATTCACAAT 
TCCAAGGAAG TGCTGAGCTT GTTGCAAGAA AAGAACCCTG CCTTTAAGCC AGTGCTTGTG 
GTTATTCAGG CCGGTGATGA CAATTTGATG AAGGATATGA ACCAGAATTT GGCCAAGGAG 
GCTGGTCTGG ACATCACTCA CATCTGCTTG CCTCCAGACA GCGGAGAGGA TGAGATCATA 
GATGAAATCC TGAAGATTAA TGAAGACCCC AGAGTGCATG GCCTTACCCT TCAGATCTCT 
GAAGATTCAT TGAGCAACAA AGTTCTCAAT GCTTTGAAAC CAGAAAAAGA TGTGGACGGG 
GTGACCGACA TCAATCTGGG GAAGCTCGTA CGAGGTGACG CTCCGGAGTG TTTTGTTTCA 
CCCTTGGCCA AAGCTGCTGT AGAACTTGTT GAAAAGTCAG GGATCACTTT GGATGGAAAG 
AAGGTCTTGG TCGTAGGGGC GCACGGGCCC CTGGAAGCTG CCCTGCAGTG GCTTTTCCAG 
AGAAAAGGAT CTATGACAAT GAGCTGCCCC TGGGCAACAC CTCAGCTTCC AGACAAGCTT 
CGAGAGGCAG ACATTGTGGT CTTAGGCTCA CCTAAGCCAG AAGAGATTCC TGCTGTGTGG 
ATTCCATCAG GAACCACCAT TCTCAATTGC TTCCATGACT TCTTGTCAGG GAAGCTGAGC 
GGAGGTTCTC CTGGGGTCCC CGTGGACAAA CTCATTGCAG AAGAGAGCGT GAGTCTCCTT 
GCTGCGGCTC TGCGCATTCA GAACATGGTG AGCAGCGGCA GGAGGTGGCT CAGAGAGCAG 
CAACATCGAA GATGGCGACT GCACTGCTTG AAACTGCAGC CTCTCTCACC TGTGCCCAGT 
GATATTGAGA TTTCTCGAGG ACAGACTCCA AAAGCTGTGG ATGTCCTTGC CAAGGAGATA 
GGATTGCTTG CAGATGAAAT TGAAATCTAT GGCAAGAGCA AAGCCAAAGT CCAATTGTCC 
CTGCTGGAGA GGTTAAAGGA TCAAACAGAT GGAAAATACG TCTTGGTTGC TGGGATCACG 
CCCACCCCGC TCGGAGAAGG GAAGAGTACA GTCACCATCG GACTAGTGCA GGCGCTGACC 
GCTCACCTGA AAGTCAACTC CTTCGCCTGT CTGAGGCAGC CTTCTCAGGG ACCCACTTTT 
GGAGTGAAAG GAGGAGCTGC AGGAGGTGGA TATGCTCAGG TCATCCCCAT GGAGGAGTTC 
AACCTTCACC TGACTGGGGA CATCCATGCC ATCACTGCCG CTAATAACTT GCTGGCCGCA 
GCCATCGACA CGAGAATTTT ACATGAGAGC ACTCAAACAG ACAAGGCTCT CTACAATCGA 
CTGGTTCCCT TAGTGAATGG TGTCAGAGAA TTTTCAGAAA TTCAACTTTC TCGGCTGAAG 
AAACTGGGGA TACATAAGAC TGATCCAAGC ACACTGACCG AGGAGGAAGT GAGGAAGTTC 
GCCCGCCTCA ACATTGACCC CGCAACCATC ACGTGGCAGA GAGTATTGGA CACGAACGAC 
CGATTTCTGA GAAAAATAAC CATTGGACAG GGAAGTACAG AGAAAGGATA TTCCCGGCAG 
GCACAGTTTG ACATTGCAGT GGCCAGCGAG ATCATGGCCG TGCTGGCCTT GACTGATAGC 
CTCACAGACA TGAAAGAGCG GCTAGGAAGA ATGGTGGTGG CCAGTGACAA AGACGGGCAG 
CCTGTGACAG CTGAGGACTT GGGGGTGACC GGCGCTCTGA CAGTTTTGAT GAAAGACGCC 
ATCAAGCCAA ATCTGATGCA GACCCTTGAA GGAACACCTG TGTTTGTGCA CGCTGGTCCC 
TTTGCCAACA TCGCTCACGG CAACTCATCA GTATTAGCTG ATAAAATCGC GCTGAAATTG 
GTCGGCGAAG AAGGGTTTGT GGAGTACACC CAGAATAGTT TCTCCATGCT CTCTGCATCA 
ACCCCAACCT TCCCCAGTGT GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mitochondrial MTHFD2L is a dual redox cofactor-specific methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase expressed in both adult and embryonic tissues.
The Journal of biological chemistry289(22)15507-17(2014 May)
Shin M,Bryant JD,Momb J,Appling DR

Deletion of Mthfd1l causes embryonic lethality and neural tube and craniofacial defects in mice.
Proceedings of the National Academy of Sciences of the United States of America110(2)549-54(2013 Jan)
Momb J,Lewandowski JP,Bryant JD,Fitch R,Surman DR,Vokes SA,Appling DR

Mitochondrial C1-tetrahydrofolate synthase (MTHFD1L) supports the flow of mitochondrial one-carbon units into the methyl cycle in embryos.
The Journal of biological chemistry285(7)4612-20(2010 Feb)
Pike ST,Rajendra R,Artzt K,Appling DR

Agouti C57BL/6N embryonic stem cells for mouse genetic resources.
Nature methods6(7)493-5(2009 Jul)
Pettitt SJ,Liang Q,Rairdan XY,Moran JL,Prosser HM,Beier DR,Lloyd KC,Bradley A,Skarnes WC

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Mthfd1l cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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