MTHFD1L cDNA ORF clone, Homo sapiens(human)

The following MTHFD1L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTHFD1L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu18141 NM_001242767.1
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu18141 XM_017010702.2
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu18141 XM_017010703.2
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu101387 NM_001350490.1
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu101378 NM_001350491.2
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00
OHu105790 XM_024446396.1
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu101389 NM_001350493.1
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $475.30
$679.00
OHu101389 NM_001350492.1
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $475.30
$679.00
OHu07102 NM_001242768.1
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu66609 XM_011535730.2
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu66610 XM_011535731.2
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu66611 XM_011535732.2
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu66611 XM_011535733.2
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu66612 XM_017010705.1
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu66612 XM_011535734.2
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu101351 NM_001350487.1
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu101372 NM_001350486.1
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu105789 XM_024446395.1
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu06995 NM_015440.4
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $265.30
$379.00
OHu07104 NM_001242769.2
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $139.30
$199.00
OHu101348 NM_001350489.2
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $139.30
$199.00
OHu101364 NM_001350488.2
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $139.30
$199.00
OHu40761 XM_005266907.5
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
OHu40762 XM_005266910.4
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
OHu40763 XM_005266911.5
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu66608 XM_011535729.3
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
OHu66615 XM_011535737.3
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu66616 XM_011535738.3
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu96523 XM_017010708.1
Latest version!
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
View Old Accession Versions >>
OHu40763 XM_005266911.4 Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu07104 NM_001242769.1 Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $139.30
$199.00
OHu101348 NM_001350489.1 Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $139.30
$199.00
OHu101378 NM_001350491.1 Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00
OHu40761 XM_005266907.4 Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
OHu66615 XM_011535737.2 Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu66616 XM_011535738.2 Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu101364 NM_001350488.1 Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $139.30
$199.00
OHu18141 XM_017010703.1 Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu18141 XM_017010702.1 Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu66608 XM_011535729.2 Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu18141
    Clone ID Related Accession (Same CDS sequence) XM_017010702.1 , NM_001242767.1 , XM_017010703.1 , XM_017010703.2 , XM_017010702.2
    Accession Version NM_001242767.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2940bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB727926.1, BC144368.1 and BC110319.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.211485.1, SRR1660803.6852.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGTG ACCGAAGCTG GCTTTGGTGC TGACATCGGA
    ATGGAGAAAT TCTTCAACAT CAAGTGCCGA GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG
    TTAGTGGCAA CGGTGCGAGC TCTGAAGATG CATGGAGGCG GGCCAAGTGT AACGGCTGGT
    GTTCCTCTTA AGAAAGAATA TACAGAGGAG AACATCCAGC TGGTGGCAGA CGGCTGCTGT
    AACCTCCAGA AGCAAATTCA GATCACTCAG CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG
    AATGTCTTCA AGACCGACAC CCGCGCTGAG ATTGACTTGG TGTGTGAGCT TGCAAAGCGG
    GCTGGTGCCT TTGATGCAGT CCCCTGCTAT CACTGGTCCG TTGGTGGAAA AGGATCGGTG
    GACTTGGCTC GGGCTGTGAG AGAGGCTGCG AGTAAAAGAA GCCGATTCCA GTTCCTGTAT
    GATGTTCAGG TTCCAATTGT GGACAAGATA AGGACCATTG CTCAGGCTGT CTATGGAGCC
    AAAGATATTG AACTCTCTCC TGAGGCACAA GCCAAAATAG ATCGTTACAC TCAACAGGGT
    TTTGGAAATT TGCCCATCTG CATGGCAAAG ACCCACCTTT CTCTATCTCA CCAACCTGAC
    AAAAAAGGTG TGCCAAGGGA CTTCATCTTA CCTATCAGTG ACGTCCGGGC CAGCATAGGC
    GCTGGGTTCA TTTACCCTTT GGTCGGAACG ATGAGCACCA TGCCAGGACT GCCCACCCGG
    CCCTGCTTTT ATGACATAGA TCTTGATACC GAAACAGAAC AAGTTAAAGG CTTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001229696.1
    CDS145..3084
    Translation

    Target ORF information:

    RefSeq Version NM_001242767.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001242767.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGTG ACCGAAGCTG GCTTTGGTGC TGACATCGGA
    ATGGAGAAAT TCTTCAACAT CAAGTGCCGA GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG
    TTAGTGGCAA CGGTGCGAGC TCTGAAGATG CATGGAGGCG GGCCAAGTGT AACGGCTGGT
    GTTCCTCTTA AGAAAGAATA TACAGAGGAG AACATCCAGC TGGTGGCAGA CGGCTGCTGT
    AACCTCCAGA AGCAAATTCA GATCACTCAG CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG
    AATGTCTTCA AGACCGACAC CCGCGCTGAG ATTGACTTGG TGTGTGAGCT TGCAAAGCGG
    GCTGGTGCCT TTGATGCAGT CCCCTGCTAT CACTGGTCCG TTGGTGGAAA AGGATCGGTG
    GACTTGGCTC GGGCTGTGAG AGAGGCTGCG AGTAAAAGAA GCCGATTCCA GTTCCTGTAT
    GATGTTCAGG TTCCAATTGT GGACAAGATA AGGACCATTG CTCAGGCTGT CTATGGAGCC
    AAAGATATTG AACTCTCTCC TGAGGCACAA GCCAAAATAG ATCGTTACAC TCAACAGGGT
    TTTGGAAATT TGCCCATCTG CATGGCAAAG ACCCACCTTT CTCTATCTCA CCAACCTGAC
    AAAAAAGGTG TGCCAAGGGA CTTCATCTTA CCTATCAGTG ACGTCCGGGC CAGCATAGGC
    GCTGGGTTCA TTTACCCTTT GGTCGGAACG ATGAGCACCA TGCCAGGACT GCCCACCCGG
    CCCTGCTTTT ATGACATAGA TCTTGATACC GAAACAGAAC AAGTTAAAGG CTTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18141
    Clone ID Related Accession (Same CDS sequence) XM_017010702.1 , NM_001242767.1 , XM_017010703.1 , XM_017010703.2 , XM_017010702.2
    Accession Version XM_017010702.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2940bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGTG ACCGAAGCTG GCTTTGGTGC TGACATCGGA
    ATGGAGAAAT TCTTCAACAT CAAGTGCCGA GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG
    TTAGTGGCAA CGGTGCGAGC TCTGAAGATG CATGGAGGCG GGCCAAGTGT AACGGCTGGT
    GTTCCTCTTA AGAAAGAATA TACAGAGGAG AACATCCAGC TGGTGGCAGA CGGCTGCTGT
    AACCTCCAGA AGCAAATTCA GATCACTCAG CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG
    AATGTCTTCA AGACCGACAC CCGCGCTGAG ATTGACTTGG TGTGTGAGCT TGCAAAGCGG
    GCTGGTGCCT TTGATGCAGT CCCCTGCTAT CACTGGTCCG TTGGTGGAAA AGGATCGGTG
    GACTTGGCTC GGGCTGTGAG AGAGGCTGCG AGTAAAAGAA GCCGATTCCA GTTCCTGTAT
    GATGTTCAGG TTCCAATTGT GGACAAGATA AGGACCATTG CTCAGGCTGT CTATGGAGCC
    AAAGATATTG AACTCTCTCC TGAGGCACAA GCCAAAATAG ATCGTTACAC TCAACAGGGT
    TTTGGAAATT TGCCCATCTG CATGGCAAAG ACCCACCTTT CTCTATCTCA CCAACCTGAC
    AAAAAAGGTG TGCCAAGGGA CTTCATCTTA CCTATCAGTG ACGTCCGGGC CAGCATAGGC
    GCTGGGTTCA TTTACCCTTT GGTCGGAACG ATGAGCACCA TGCCAGGACT GCCCACCCGG
    CCCTGCTTTT ATGACATAGA TCTTGATACC GAAACAGAAC AAGTTAAAGG CTTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866191.1
    CDS85..3024
    Translation

    Target ORF information:

    RefSeq Version XM_017010702.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010702.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGTG ACCGAAGCTG GCTTTGGTGC TGACATCGGA
    ATGGAGAAAT TCTTCAACAT CAAGTGCCGA GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG
    TTAGTGGCAA CGGTGCGAGC TCTGAAGATG CATGGAGGCG GGCCAAGTGT AACGGCTGGT
    GTTCCTCTTA AGAAAGAATA TACAGAGGAG AACATCCAGC TGGTGGCAGA CGGCTGCTGT
    AACCTCCAGA AGCAAATTCA GATCACTCAG CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG
    AATGTCTTCA AGACCGACAC CCGCGCTGAG ATTGACTTGG TGTGTGAGCT TGCAAAGCGG
    GCTGGTGCCT TTGATGCAGT CCCCTGCTAT CACTGGTCCG TTGGTGGAAA AGGATCGGTG
    GACTTGGCTC GGGCTGTGAG AGAGGCTGCG AGTAAAAGAA GCCGATTCCA GTTCCTGTAT
    GATGTTCAGG TTCCAATTGT GGACAAGATA AGGACCATTG CTCAGGCTGT CTATGGAGCC
    AAAGATATTG AACTCTCTCC TGAGGCACAA GCCAAAATAG ATCGTTACAC TCAACAGGGT
    TTTGGAAATT TGCCCATCTG CATGGCAAAG ACCCACCTTT CTCTATCTCA CCAACCTGAC
    AAAAAAGGTG TGCCAAGGGA CTTCATCTTA CCTATCAGTG ACGTCCGGGC CAGCATAGGC
    GCTGGGTTCA TTTACCCTTT GGTCGGAACG ATGAGCACCA TGCCAGGACT GCCCACCCGG
    CCCTGCTTTT ATGACATAGA TCTTGATACC GAAACAGAAC AAGTTAAAGG CTTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18141
    Clone ID Related Accession (Same CDS sequence) XM_017010702.1 , NM_001242767.1 , XM_017010703.1 , XM_017010703.2 , XM_017010702.2
    Accession Version XM_017010703.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2940bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGTG ACCGAAGCTG GCTTTGGTGC TGACATCGGA
    ATGGAGAAAT TCTTCAACAT CAAGTGCCGA GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG
    TTAGTGGCAA CGGTGCGAGC TCTGAAGATG CATGGAGGCG GGCCAAGTGT AACGGCTGGT
    GTTCCTCTTA AGAAAGAATA TACAGAGGAG AACATCCAGC TGGTGGCAGA CGGCTGCTGT
    AACCTCCAGA AGCAAATTCA GATCACTCAG CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG
    AATGTCTTCA AGACCGACAC CCGCGCTGAG ATTGACTTGG TGTGTGAGCT TGCAAAGCGG
    GCTGGTGCCT TTGATGCAGT CCCCTGCTAT CACTGGTCCG TTGGTGGAAA AGGATCGGTG
    GACTTGGCTC GGGCTGTGAG AGAGGCTGCG AGTAAAAGAA GCCGATTCCA GTTCCTGTAT
    GATGTTCAGG TTCCAATTGT GGACAAGATA AGGACCATTG CTCAGGCTGT CTATGGAGCC
    AAAGATATTG AACTCTCTCC TGAGGCACAA GCCAAAATAG ATCGTTACAC TCAACAGGGT
    TTTGGAAATT TGCCCATCTG CATGGCAAAG ACCCACCTTT CTCTATCTCA CCAACCTGAC
    AAAAAAGGTG TGCCAAGGGA CTTCATCTTA CCTATCAGTG ACGTCCGGGC CAGCATAGGC
    GCTGGGTTCA TTTACCCTTT GGTCGGAACG ATGAGCACCA TGCCAGGACT GCCCACCCGG
    CCCTGCTTTT ATGACATAGA TCTTGATACC GAAACAGAAC AAGTTAAAGG CTTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866192.1
    CDS85..3024
    Translation

    Target ORF information:

    RefSeq Version XM_017010703.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010703.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGTG ACCGAAGCTG GCTTTGGTGC TGACATCGGA
    ATGGAGAAAT TCTTCAACAT CAAGTGCCGA GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG
    TTAGTGGCAA CGGTGCGAGC TCTGAAGATG CATGGAGGCG GGCCAAGTGT AACGGCTGGT
    GTTCCTCTTA AGAAAGAATA TACAGAGGAG AACATCCAGC TGGTGGCAGA CGGCTGCTGT
    AACCTCCAGA AGCAAATTCA GATCACTCAG CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG
    AATGTCTTCA AGACCGACAC CCGCGCTGAG ATTGACTTGG TGTGTGAGCT TGCAAAGCGG
    GCTGGTGCCT TTGATGCAGT CCCCTGCTAT CACTGGTCCG TTGGTGGAAA AGGATCGGTG
    GACTTGGCTC GGGCTGTGAG AGAGGCTGCG AGTAAAAGAA GCCGATTCCA GTTCCTGTAT
    GATGTTCAGG TTCCAATTGT GGACAAGATA AGGACCATTG CTCAGGCTGT CTATGGAGCC
    AAAGATATTG AACTCTCTCC TGAGGCACAA GCCAAAATAG ATCGTTACAC TCAACAGGGT
    TTTGGAAATT TGCCCATCTG CATGGCAAAG ACCCACCTTT CTCTATCTCA CCAACCTGAC
    AAAAAAGGTG TGCCAAGGGA CTTCATCTTA CCTATCAGTG ACGTCCGGGC CAGCATAGGC
    GCTGGGTTCA TTTACCCTTT GGTCGGAACG ATGAGCACCA TGCCAGGACT GCCCACCCGG
    CCCTGCTTTT ATGACATAGA TCTTGATACC GAAACAGAAC AAGTTAAAGG CTTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18141
    Clone ID Related Accession (Same CDS sequence) XM_017010702.1 , NM_001242767.1 , XM_017010703.1 , XM_017010703.2 , XM_017010702.2
    Accession Version XM_017010703.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2940bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017010703.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGTG ACCGAAGCTG GCTTTGGTGC TGACATCGGA
    ATGGAGAAAT TCTTCAACAT CAAGTGCCGA GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG
    TTAGTGGCAA CGGTGCGAGC TCTGAAGATG CATGGAGGCG GGCCAAGTGT AACGGCTGGT
    GTTCCTCTTA AGAAAGAATA TACAGAGGAG AACATCCAGC TGGTGGCAGA CGGCTGCTGT
    AACCTCCAGA AGCAAATTCA GATCACTCAG CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG
    AATGTCTTCA AGACCGACAC CCGCGCTGAG ATTGACTTGG TGTGTGAGCT TGCAAAGCGG
    GCTGGTGCCT TTGATGCAGT CCCCTGCTAT CACTGGTCCG TTGGTGGAAA AGGATCGGTG
    GACTTGGCTC GGGCTGTGAG AGAGGCTGCG AGTAAAAGAA GCCGATTCCA GTTCCTGTAT
    GATGTTCAGG TTCCAATTGT GGACAAGATA AGGACCATTG CTCAGGCTGT CTATGGAGCC
    AAAGATATTG AACTCTCTCC TGAGGCACAA GCCAAAATAG ATCGTTACAC TCAACAGGGT
    TTTGGAAATT TGCCCATCTG CATGGCAAAG ACCCACCTTT CTCTATCTCA CCAACCTGAC
    AAAAAAGGTG TGCCAAGGGA CTTCATCTTA CCTATCAGTG ACGTCCGGGC CAGCATAGGC
    GCTGGGTTCA TTTACCCTTT GGTCGGAACG ATGAGCACCA TGCCAGGACT GCCCACCCGG
    CCCTGCTTTT ATGACATAGA TCTTGATACC GAAACAGAAC AAGTTAAAGG CTTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866192.1
    CDS140..3079
    Translation

    Target ORF information:

    RefSeq Version XM_017010703.2
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010703.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGTG ACCGAAGCTG GCTTTGGTGC TGACATCGGA
    ATGGAGAAAT TCTTCAACAT CAAGTGCCGA GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG
    TTAGTGGCAA CGGTGCGAGC TCTGAAGATG CATGGAGGCG GGCCAAGTGT AACGGCTGGT
    GTTCCTCTTA AGAAAGAATA TACAGAGGAG AACATCCAGC TGGTGGCAGA CGGCTGCTGT
    AACCTCCAGA AGCAAATTCA GATCACTCAG CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG
    AATGTCTTCA AGACCGACAC CCGCGCTGAG ATTGACTTGG TGTGTGAGCT TGCAAAGCGG
    GCTGGTGCCT TTGATGCAGT CCCCTGCTAT CACTGGTCCG TTGGTGGAAA AGGATCGGTG
    GACTTGGCTC GGGCTGTGAG AGAGGCTGCG AGTAAAAGAA GCCGATTCCA GTTCCTGTAT
    GATGTTCAGG TTCCAATTGT GGACAAGATA AGGACCATTG CTCAGGCTGT CTATGGAGCC
    AAAGATATTG AACTCTCTCC TGAGGCACAA GCCAAAATAG ATCGTTACAC TCAACAGGGT
    TTTGGAAATT TGCCCATCTG CATGGCAAAG ACCCACCTTT CTCTATCTCA CCAACCTGAC
    AAAAAAGGTG TGCCAAGGGA CTTCATCTTA CCTATCAGTG ACGTCCGGGC CAGCATAGGC
    GCTGGGTTCA TTTACCCTTT GGTCGGAACG ATGAGCACCA TGCCAGGACT GCCCACCCGG
    CCCTGCTTTT ATGACATAGA TCTTGATACC GAAACAGAAC AAGTTAAAGG CTTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18141
    Clone ID Related Accession (Same CDS sequence) XM_017010702.1 , NM_001242767.1 , XM_017010703.1 , XM_017010703.2 , XM_017010702.2
    Accession Version XM_017010702.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2940bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017010702.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGTG ACCGAAGCTG GCTTTGGTGC TGACATCGGA
    ATGGAGAAAT TCTTCAACAT CAAGTGCCGA GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG
    TTAGTGGCAA CGGTGCGAGC TCTGAAGATG CATGGAGGCG GGCCAAGTGT AACGGCTGGT
    GTTCCTCTTA AGAAAGAATA TACAGAGGAG AACATCCAGC TGGTGGCAGA CGGCTGCTGT
    AACCTCCAGA AGCAAATTCA GATCACTCAG CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG
    AATGTCTTCA AGACCGACAC CCGCGCTGAG ATTGACTTGG TGTGTGAGCT TGCAAAGCGG
    GCTGGTGCCT TTGATGCAGT CCCCTGCTAT CACTGGTCCG TTGGTGGAAA AGGATCGGTG
    GACTTGGCTC GGGCTGTGAG AGAGGCTGCG AGTAAAAGAA GCCGATTCCA GTTCCTGTAT
    GATGTTCAGG TTCCAATTGT GGACAAGATA AGGACCATTG CTCAGGCTGT CTATGGAGCC
    AAAGATATTG AACTCTCTCC TGAGGCACAA GCCAAAATAG ATCGTTACAC TCAACAGGGT
    TTTGGAAATT TGCCCATCTG CATGGCAAAG ACCCACCTTT CTCTATCTCA CCAACCTGAC
    AAAAAAGGTG TGCCAAGGGA CTTCATCTTA CCTATCAGTG ACGTCCGGGC CAGCATAGGC
    GCTGGGTTCA TTTACCCTTT GGTCGGAACG ATGAGCACCA TGCCAGGACT GCCCACCCGG
    CCCTGCTTTT ATGACATAGA TCTTGATACC GAAACAGAAC AAGTTAAAGG CTTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866191.1
    CDS140..3079
    Translation

    Target ORF information:

    RefSeq Version XM_017010702.2
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010702.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGTG ACCGAAGCTG GCTTTGGTGC TGACATCGGA
    ATGGAGAAAT TCTTCAACAT CAAGTGCCGA GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG
    TTAGTGGCAA CGGTGCGAGC TCTGAAGATG CATGGAGGCG GGCCAAGTGT AACGGCTGGT
    GTTCCTCTTA AGAAAGAATA TACAGAGGAG AACATCCAGC TGGTGGCAGA CGGCTGCTGT
    AACCTCCAGA AGCAAATTCA GATCACTCAG CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG
    AATGTCTTCA AGACCGACAC CCGCGCTGAG ATTGACTTGG TGTGTGAGCT TGCAAAGCGG
    GCTGGTGCCT TTGATGCAGT CCCCTGCTAT CACTGGTCCG TTGGTGGAAA AGGATCGGTG
    GACTTGGCTC GGGCTGTGAG AGAGGCTGCG AGTAAAAGAA GCCGATTCCA GTTCCTGTAT
    GATGTTCAGG TTCCAATTGT GGACAAGATA AGGACCATTG CTCAGGCTGT CTATGGAGCC
    AAAGATATTG AACTCTCTCC TGAGGCACAA GCCAAAATAG ATCGTTACAC TCAACAGGGT
    TTTGGAAATT TGCCCATCTG CATGGCAAAG ACCCACCTTT CTCTATCTCA CCAACCTGAC
    AAAAAAGGTG TGCCAAGGGA CTTCATCTTA CCTATCAGTG ACGTCCGGGC CAGCATAGGC
    GCTGGGTTCA TTTACCCTTT GGTCGGAACG ATGAGCACCA TGCCAGGACT GCCCACCCGG
    CCCTGCTTTT ATGACATAGA TCTTGATACC GAAACAGAAC AAGTTAAAGG CTTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101387
    Clone ID Related Accession (Same CDS sequence) NM_001350490.1
    Accession Version NM_001350490.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-15
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform 9
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL049694.9 and AL133260.12. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.254122.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGACCGAAG CTGGCTTTGG TGCTGACATC GGAATGGAGA AATTCTTCAA CATCAAGTGC 
    CGAGCTTCCG GCTTGGTGCC CAACGTGGTT GTGTTAGTGG CAACGGTGCG AGCTCTGAAG
    ATGCATGGAG GCGGGCCAAG TGTAACGGCT GGTGTTCCTC TTAAGAAAGA ATATACAGAG
    GAGAACATCC AGCTGGTGGC AGACGGCTGC TGTAACCTCC AGAAGCAAAT TCAGATCACT
    CAGCTCTTTG GGGTTCCCGT TGTGGTGGCT CTGAATGTCT TCAAGACCGA CACCCGCGCT
    GAGATTGACT TGGTGTGTGA GCTTGCAAAG CGGGCTGGTG CCTTTGATGC AGTCCCCTGC
    TATCACTGGT CCGTTGGTGG AAAAGGATCG GTGGACTTGG CTCGGGCTGT GAGAGAGGCT
    GCGAGTAAAA GAAGCCGATT CCAGTTCCTG TATGATGTTC AGGTTCCAAT TGTGGACAAG
    ATAAGGACCA TTGCTCAGGC TGTCTATGGA GCCAAAGATA TTGAACTCTC TCCTGAGGCA
    CAAGCCAAAA TAGATCGTTA CACTCAACAG GGTTTTGGAA ATTTGCCCAT CTGCATGGCA
    AAGACCCACC TTTCTCTATC TCACCAACCT GACAAAAAAG GTGTGCCAAG GGACTTCATC
    TTACCTATCA GTGACGTCCG GGCCAGCATA GGCGCTGGGT TCATTTACCC TTTGGTCGGA
    ACGATGAGCA CCATGCCAGG ACTGCCCACC CGGCCCTGCT TTTATGACAT AGATCTTGAT
    ACCGAAACAG AACAAGTTAA AGGCTTGTTC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337419.1
    CDS811..1623
    Translation

    Target ORF information:

    RefSeq Version NM_001350490.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350490.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGACCGAAG CTGGCTTTGG TGCTGACATC GGAATGGAGA AATTCTTCAA CATCAAGTGC 
    CGAGCTTCCG GCTTGGTGCC CAACGTGGTT GTGTTAGTGG CAACGGTGCG AGCTCTGAAG
    ATGCATGGAG GCGGGCCAAG TGTAACGGCT GGTGTTCCTC TTAAGAAAGA ATATACAGAG
    GAGAACATCC AGCTGGTGGC AGACGGCTGC TGTAACCTCC AGAAGCAAAT TCAGATCACT
    CAGCTCTTTG GGGTTCCCGT TGTGGTGGCT CTGAATGTCT TCAAGACCGA CACCCGCGCT
    GAGATTGACT TGGTGTGTGA GCTTGCAAAG CGGGCTGGTG CCTTTGATGC AGTCCCCTGC
    TATCACTGGT CCGTTGGTGG AAAAGGATCG GTGGACTTGG CTCGGGCTGT GAGAGAGGCT
    GCGAGTAAAA GAAGCCGATT CCAGTTCCTG TATGATGTTC AGGTTCCAAT TGTGGACAAG
    ATAAGGACCA TTGCTCAGGC TGTCTATGGA GCCAAAGATA TTGAACTCTC TCCTGAGGCA
    CAAGCCAAAA TAGATCGTTA CACTCAACAG GGTTTTGGAA ATTTGCCCAT CTGCATGGCA
    AAGACCCACC TTTCTCTATC TCACCAACCT GACAAAAAAG GTGTGCCAAG GGACTTCATC
    TTACCTATCA GTGACGTCCG GGCCAGCATA GGCGCTGGGT TCATTTACCC TTTGGTCGGA
    ACGATGAGCA CCATGCCAGG ACTGCCCACC CGGCCCTGCT TTTATGACAT AGATCTTGAT
    ACCGAAACAG AACAAGTTAA AGGCTTGTTC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101378
    Clone ID Related Accession (Same CDS sequence) NM_001350491.1 , NM_001350491.2
    Accession Version NM_001350491.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform 10
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL035086.12. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK310342.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGCAGGAAA TCAACCAGAA TTTGGCTGAG GAGGCTGGTC TGAACATCAC TCACATTTGC 
    CTCCCTCCAG ATAGCAGTGA AGCCGAGATT ATAGATGAAA TCTTAAAGAT CAATGAAGAT
    ACCAGAGTAC ATGGCCTTGC CCTTCAGATC TCTGAGAACT TGTTTAGCAA CAAAGTCCTC
    AATGCCTTGA AACCAGAAAA AGATGTGGAT GGAGTAACAG ACATAAACCT GGGGAAGCTG
    GTGCGAGGGG ATGCCCATGA ATGTTTTGTT TCACCTGTTG CCAAAGCTGT AATTGAACTT
    CTTGAAAAAT CAGTAGGTGT CAACCTAGAT GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT
    GGGTCTTTGG AAGCTGCTCT ACAATGCCTG TTCCAGAGAA AAGGGTCCAT GACAATGAGC
    ATCCAGTGGA AAACACGCCA GCTTCAAAGC AAGACGGAGT CTCGTTCTGT CACCAGGCTG
    GAGTGCAGGC GCGTGATCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337420.1
    CDS1240..1740
    Translation

    Target ORF information:

    RefSeq Version NM_001350491.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350491.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGCAGGAAA TCAACCAGAA TTTGGCTGAG GAGGCTGGTC TGAACATCAC TCACATTTGC 
    CTCCCTCCAG ATAGCAGTGA AGCCGAGATT ATAGATGAAA TCTTAAAGAT CAATGAAGAT
    ACCAGAGTAC ATGGCCTTGC CCTTCAGATC TCTGAGAACT TGTTTAGCAA CAAAGTCCTC
    AATGCCTTGA AACCAGAAAA AGATGTGGAT GGAGTAACAG ACATAAACCT GGGGAAGCTG
    GTGCGAGGGG ATGCCCATGA ATGTTTTGTT TCACCTGTTG CCAAAGCTGT AATTGAACTT
    CTTGAAAAAT CAGTAGGTGT CAACCTAGAT GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT
    GGGTCTTTGG AAGCTGCTCT ACAATGCCTG TTCCAGAGAA AAGGGTCCAT GACAATGAGC
    ATCCAGTGGA AAACACGCCA GCTTCAAAGC AAGACGGAGT CTCGTTCTGT CACCAGGCTG
    GAGTGCAGGC GCGTGATCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101378
    Clone ID Related Accession (Same CDS sequence) NM_001350491.1 , NM_001350491.2
    Accession Version NM_001350491.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-18
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform 10
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL035086.12. On Jun 2, 2019 this sequence version replaced NM_001350491.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK310342.1, SRR3476690.735584.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGCAGGAAA TCAACCAGAA TTTGGCTGAG GAGGCTGGTC TGAACATCAC TCACATTTGC 
    CTCCCTCCAG ATAGCAGTGA AGCCGAGATT ATAGATGAAA TCTTAAAGAT CAATGAAGAT
    ACCAGAGTAC ATGGCCTTGC CCTTCAGATC TCTGAGAACT TGTTTAGCAA CAAAGTCCTC
    AATGCCTTGA AACCAGAAAA AGATGTGGAT GGAGTAACAG ACATAAACCT GGGGAAGCTG
    GTGCGAGGGG ATGCCCATGA ATGTTTTGTT TCACCTGTTG CCAAAGCTGT AATTGAACTT
    CTTGAAAAAT CAGTAGGTGT CAACCTAGAT GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT
    GGGTCTTTGG AAGCTGCTCT ACAATGCCTG TTCCAGAGAA AAGGGTCCAT GACAATGAGC
    ATCCAGTGGA AAACACGCCA GCTTCAAAGC AAGACGGAGT CTCGTTCTGT CACCAGGCTG
    GAGTGCAGGC GCGTGATCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337420.1
    CDS1201..1701
    Translation

    Target ORF information:

    RefSeq Version NM_001350491.2
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350491.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGCAGGAAA TCAACCAGAA TTTGGCTGAG GAGGCTGGTC TGAACATCAC TCACATTTGC 
    CTCCCTCCAG ATAGCAGTGA AGCCGAGATT ATAGATGAAA TCTTAAAGAT CAATGAAGAT
    ACCAGAGTAC ATGGCCTTGC CCTTCAGATC TCTGAGAACT TGTTTAGCAA CAAAGTCCTC
    AATGCCTTGA AACCAGAAAA AGATGTGGAT GGAGTAACAG ACATAAACCT GGGGAAGCTG
    GTGCGAGGGG ATGCCCATGA ATGTTTTGTT TCACCTGTTG CCAAAGCTGT AATTGAACTT
    CTTGAAAAAT CAGTAGGTGT CAACCTAGAT GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT
    GGGTCTTTGG AAGCTGCTCT ACAATGCCTG TTCCAGAGAA AAGGGTCCAT GACAATGAGC
    ATCCAGTGGA AAACACGCCA GCTTCAAAGC AAGACGGAGT CTCGTTCTGT CACCAGGCTG
    GAGTGCAGGC GCGTGATCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu105790
    Clone ID Related Accession (Same CDS sequence) XM_024446396.1
    Accession Version XM_024446396.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1311bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    TTTTGTAGAT CAGAAAACTC TATTAGATTG GTTGTGGTTG AGTATCCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024302164.1
    CDS140..1450
    Translation

    Target ORF information:

    RefSeq Version XM_024446396.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024446396.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    TTTTGTAGAT CAGAAAACTC TATTAGATTG GTTGTGGTTG AGTATCCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101389
    Clone ID Related Accession (Same CDS sequence) NM_001350493.1 , NM_001350492.1
    Accession Version NM_001350493.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2610bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-16
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform 11
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL035086.12, AL049694.9 and AL133260.12. Transcript Variant: This variant (12), as well as variant 11, encodes isoform 11. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.95886.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146411, SAMEA2146982 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGGAAA TCAACCAGAA TTTGGCTGAG GAGGCTGGTC TGAACATCAC TCACATTTGC 
    CTCCCTCCAG ATAGCAGTGA AGCCGAGATT ATAGATGAAA TCTTAAAGAT CAATGAAGAT
    ACCAGAGTAC ATGGCCTTGC CCTTCAGATC TCTGAGAACT TGTTTAGCAA CAAAGTCCTC
    AATGCCTTGA AACCAGAAAA AGATGTGGAT GGAGTAACAG ACATAAACCT GGGGAAGCTG
    GTGCGAGGGG ATGCCCATGA ATGTTTTGTT TCACCTGTTG CCAAAGCTGT AATTGAACTT
    CTTGAAAAAT CAGTAGGTGT CAACCTAGAT GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT
    GGGTCTTTGG AAGCTGCTCT ACAATGCCTG TTCCAGAGAA AAGGGTCCAT GACAATGAGC
    ATCCAGTGGA AAACACGCCA GCTTCAAAGC AAGCTTCACG AGGCTGACAT TGTGGTCCTA
    GGCTCACCTA AGCCAGAAGA GATTCCCCTT ACTTGGATAC AACCAGGAAC TACTGTTCTC
    AACTGCTCCC ATGACTTCCT GTCAGGGAAG GTTGGGTGTG GCTCTCCAAG AATACATTTT
    GGTGGACTCA TTGAGGAAGA TGATGTGATT CTCCTTGCTG CAGCTCTGCG AATTCAGAAC
    ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT GAACAGCAGC ACAGGCGGTG GAGACTTCAC
    TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG CCAAGTGACA TTGAGATTTC AAGAGGACAA
    ACTCCAAAAG CTGTGGATGT CCTTGCCAAG GAGATTGGAT TGCTTGCAGA TGAAATTGAA
    ATCTATGGCA AAAGCAAAGC CAAAGTACGT TTGTCCGTGC TAGAAAGGTT AAAGGATCAA
    GCAGATGGAA AATACGTCTT AGTTGCTGGG ATCACACCCA CCCCTCTTGG AGAAGGGAAG
    AGCACAGTCA CCATCGGGCT TGTGCAGGCT CTGACCGCAC ACCTGAATGT CAACTCCTTT
    GCCTGCTTGA GGCAGCCTTC CCAAGGACCG ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT
    GGTGGATATG CCCAGGTCAT CCCCATGGAG GAGTTCAACC TTCACTTGAC TGGAGACATC
    CACGCCATCA CCGCTGCCAA TAACTTGCTG GCTGCCGCCA TCGACACGAG GATTCTTCAT
    GAAAACACGC AAACAGATAA GGCTCTGTAT AATCGGCTGG TTCCTTTAGT GAATGGTGTC
    AGAGAATTTT CAGAAATTCA GCTTGCTCGG CTAAAAAAAC TGGGAATAAA TAAGACTGAT
    CCGAGCACAC TGACAGAAGA GGAAGTGAGT AAATTTGCCC GTCTCGACAT CGACCCATCT
    ACCATCACGT GGCAGAGAGT ATTGGATACA AATGACCGAT TTCTACGAAA AATAACCATC
    GGGCAGGGAA ACACAGAGAA GGGCCATTAC CGGCAGGCGC AGTTTGACAT CGCAGTGGCC
    AGCGAGATCA TGGCGGTGCT GGCCCTGACG GACAGCCTCG CAGACATGAA GGCACGGCTG
    GGAAGGATGG TGGTGGCCAG TGACAAAAGC GGGCAGCCTG TGACAGCAGA TGATTTGGGG
    GTGACAGGTG CTTTGACAGT TTTGATGAAA GATGCAATAA AACCAAACCT GATGCAGACC
    CTGGAAGGGA CACCTGTGTT CGTGCATGCG GGCCCTTTTG CTAACATTGC TCACGGCAAC
    TCTTCAGTGT TGGCTGATAA AATTGCCCTG AAACTGGTTG GTGAAGAAGG ATTTGTAGTG
    ACCGAAGCTG GCTTTGGTGC TGACATCGGA ATGGAGAAAT TCTTCAACAT CAAGTGCCGA
    GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG TTAGTGGCAA CGGTGCGAGC TCTGAAGATG
    CATGGAGGCG GGCCAAGTGT AACGGCTGGT GTTCCTCTTA AGAAAGAATA TACAGAGGAG
    AACATCCAGC TGGTGGCAGA CGGCTGCTGT AACCTCCAGA AGCAAATTCA GATCACTCAG
    CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG AATGTCTTCA AGACCGACAC CCGCGCTGAG
    ATTGACTTGG TGTGTGAGCT TGCAAAGCGG GCTGGTGCCT TTGATGCAGT CCCCTGCTAT
    CACTGGTCCG TTGGTGGAAA AGGATCGGTG GACTTGGCTC GGGCTGTGAG AGAGGCTGCG
    AGTAAAAGAA GCCGATTCCA GTTCCTGTAT GATGTTCAGG TTCCAATTGT GGACAAGATA
    AGGACCATTG CTCAGGCTGT CTATGGAGCC AAAGATATTG AACTCTCTCC TGAGGCACAA
    GCCAAAATAG ATCGTTACAC TCAACAGGGT TTTGGAAATT TGCCCATCTG CATGGCAAAG
    ACCCACCTTT CTCTATCTCA CCAACCTGAC AAAAAAGGTG TGCCAAGGGA CTTCATCTTA
    CCTATCAGTG ACGTCCGGGC CAGCATAGGC GCTGGGTTCA TTTACCCTTT GGTCGGAACG
    ATGAGCACCA TGCCAGGACT GCCCACCCGG CCCTGCTTTT ATGACATAGA TCTTGATACC
    GAAACAGAAC AAGTTAAAGG CTTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337422.1
    CDS337..2946
    Translation

    Target ORF information:

    RefSeq Version NM_001350493.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350493.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGGAAA TCAACCAGAA TTTGGCTGAG GAGGCTGGTC TGAACATCAC TCACATTTGC 
    CTCCCTCCAG ATAGCAGTGA AGCCGAGATT ATAGATGAAA TCTTAAAGAT CAATGAAGAT
    ACCAGAGTAC ATGGCCTTGC CCTTCAGATC TCTGAGAACT TGTTTAGCAA CAAAGTCCTC
    AATGCCTTGA AACCAGAAAA AGATGTGGAT GGAGTAACAG ACATAAACCT GGGGAAGCTG
    GTGCGAGGGG ATGCCCATGA ATGTTTTGTT TCACCTGTTG CCAAAGCTGT AATTGAACTT
    CTTGAAAAAT CAGTAGGTGT CAACCTAGAT GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT
    GGGTCTTTGG AAGCTGCTCT ACAATGCCTG TTCCAGAGAA AAGGGTCCAT GACAATGAGC
    ATCCAGTGGA AAACACGCCA GCTTCAAAGC AAGCTTCACG AGGCTGACAT TGTGGTCCTA
    GGCTCACCTA AGCCAGAAGA GATTCCCCTT ACTTGGATAC AACCAGGAAC TACTGTTCTC
    AACTGCTCCC ATGACTTCCT GTCAGGGAAG GTTGGGTGTG GCTCTCCAAG AATACATTTT
    GGTGGACTCA TTGAGGAAGA TGATGTGATT CTCCTTGCTG CAGCTCTGCG AATTCAGAAC
    ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT GAACAGCAGC ACAGGCGGTG GAGACTTCAC
    TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG CCAAGTGACA TTGAGATTTC AAGAGGACAA
    ACTCCAAAAG CTGTGGATGT CCTTGCCAAG GAGATTGGAT TGCTTGCAGA TGAAATTGAA
    ATCTATGGCA AAAGCAAAGC CAAAGTACGT TTGTCCGTGC TAGAAAGGTT AAAGGATCAA
    GCAGATGGAA AATACGTCTT AGTTGCTGGG ATCACACCCA CCCCTCTTGG AGAAGGGAAG
    AGCACAGTCA CCATCGGGCT TGTGCAGGCT CTGACCGCAC ACCTGAATGT CAACTCCTTT
    GCCTGCTTGA GGCAGCCTTC CCAAGGACCG ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT
    GGTGGATATG CCCAGGTCAT CCCCATGGAG GAGTTCAACC TTCACTTGAC TGGAGACATC
    CACGCCATCA CCGCTGCCAA TAACTTGCTG GCTGCCGCCA TCGACACGAG GATTCTTCAT
    GAAAACACGC AAACAGATAA GGCTCTGTAT AATCGGCTGG TTCCTTTAGT GAATGGTGTC
    AGAGAATTTT CAGAAATTCA GCTTGCTCGG CTAAAAAAAC TGGGAATAAA TAAGACTGAT
    CCGAGCACAC TGACAGAAGA GGAAGTGAGT AAATTTGCCC GTCTCGACAT CGACCCATCT
    ACCATCACGT GGCAGAGAGT ATTGGATACA AATGACCGAT TTCTACGAAA AATAACCATC
    GGGCAGGGAA ACACAGAGAA GGGCCATTAC CGGCAGGCGC AGTTTGACAT CGCAGTGGCC
    AGCGAGATCA TGGCGGTGCT GGCCCTGACG GACAGCCTCG CAGACATGAA GGCACGGCTG
    GGAAGGATGG TGGTGGCCAG TGACAAAAGC GGGCAGCCTG TGACAGCAGA TGATTTGGGG
    GTGACAGGTG CTTTGACAGT TTTGATGAAA GATGCAATAA AACCAAACCT GATGCAGACC
    CTGGAAGGGA CACCTGTGTT CGTGCATGCG GGCCCTTTTG CTAACATTGC TCACGGCAAC
    TCTTCAGTGT TGGCTGATAA AATTGCCCTG AAACTGGTTG GTGAAGAAGG ATTTGTAGTG
    ACCGAAGCTG GCTTTGGTGC TGACATCGGA ATGGAGAAAT TCTTCAACAT CAAGTGCCGA
    GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG TTAGTGGCAA CGGTGCGAGC TCTGAAGATG
    CATGGAGGCG GGCCAAGTGT AACGGCTGGT GTTCCTCTTA AGAAAGAATA TACAGAGGAG
    AACATCCAGC TGGTGGCAGA CGGCTGCTGT AACCTCCAGA AGCAAATTCA GATCACTCAG
    CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG AATGTCTTCA AGACCGACAC CCGCGCTGAG
    ATTGACTTGG TGTGTGAGCT TGCAAAGCGG GCTGGTGCCT TTGATGCAGT CCCCTGCTAT
    CACTGGTCCG TTGGTGGAAA AGGATCGGTG GACTTGGCTC GGGCTGTGAG AGAGGCTGCG
    AGTAAAAGAA GCCGATTCCA GTTCCTGTAT GATGTTCAGG TTCCAATTGT GGACAAGATA
    AGGACCATTG CTCAGGCTGT CTATGGAGCC AAAGATATTG AACTCTCTCC TGAGGCACAA
    GCCAAAATAG ATCGTTACAC TCAACAGGGT TTTGGAAATT TGCCCATCTG CATGGCAAAG
    ACCCACCTTT CTCTATCTCA CCAACCTGAC AAAAAAGGTG TGCCAAGGGA CTTCATCTTA
    CCTATCAGTG ACGTCCGGGC CAGCATAGGC GCTGGGTTCA TTTACCCTTT GGTCGGAACG
    ATGAGCACCA TGCCAGGACT GCCCACCCGG CCCTGCTTTT ATGACATAGA TCTTGATACC
    GAAACAGAAC AAGTTAAAGG CTTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101389
    Clone ID Related Accession (Same CDS sequence) NM_001350493.1 , NM_001350492.1
    Accession Version NM_001350492.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2610bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-15
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform 11
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL035086.12, AL049694.9 and AL133260.12. On Apr 14, 2017 this sequence version replaced XM_017010706.1. Transcript Variant: This variant (11), as well as variant 12, encodes isoform 11. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.220054.1, SRR1803616.185805.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGGAAA TCAACCAGAA TTTGGCTGAG GAGGCTGGTC TGAACATCAC TCACATTTGC 
    CTCCCTCCAG ATAGCAGTGA AGCCGAGATT ATAGATGAAA TCTTAAAGAT CAATGAAGAT
    ACCAGAGTAC ATGGCCTTGC CCTTCAGATC TCTGAGAACT TGTTTAGCAA CAAAGTCCTC
    AATGCCTTGA AACCAGAAAA AGATGTGGAT GGAGTAACAG ACATAAACCT GGGGAAGCTG
    GTGCGAGGGG ATGCCCATGA ATGTTTTGTT TCACCTGTTG CCAAAGCTGT AATTGAACTT
    CTTGAAAAAT CAGTAGGTGT CAACCTAGAT GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT
    GGGTCTTTGG AAGCTGCTCT ACAATGCCTG TTCCAGAGAA AAGGGTCCAT GACAATGAGC
    ATCCAGTGGA AAACACGCCA GCTTCAAAGC AAGCTTCACG AGGCTGACAT TGTGGTCCTA
    GGCTCACCTA AGCCAGAAGA GATTCCCCTT ACTTGGATAC AACCAGGAAC TACTGTTCTC
    AACTGCTCCC ATGACTTCCT GTCAGGGAAG GTTGGGTGTG GCTCTCCAAG AATACATTTT
    GGTGGACTCA TTGAGGAAGA TGATGTGATT CTCCTTGCTG CAGCTCTGCG AATTCAGAAC
    ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT GAACAGCAGC ACAGGCGGTG GAGACTTCAC
    TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG CCAAGTGACA TTGAGATTTC AAGAGGACAA
    ACTCCAAAAG CTGTGGATGT CCTTGCCAAG GAGATTGGAT TGCTTGCAGA TGAAATTGAA
    ATCTATGGCA AAAGCAAAGC CAAAGTACGT TTGTCCGTGC TAGAAAGGTT AAAGGATCAA
    GCAGATGGAA AATACGTCTT AGTTGCTGGG ATCACACCCA CCCCTCTTGG AGAAGGGAAG
    AGCACAGTCA CCATCGGGCT TGTGCAGGCT CTGACCGCAC ACCTGAATGT CAACTCCTTT
    GCCTGCTTGA GGCAGCCTTC CCAAGGACCG ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT
    GGTGGATATG CCCAGGTCAT CCCCATGGAG GAGTTCAACC TTCACTTGAC TGGAGACATC
    CACGCCATCA CCGCTGCCAA TAACTTGCTG GCTGCCGCCA TCGACACGAG GATTCTTCAT
    GAAAACACGC AAACAGATAA GGCTCTGTAT AATCGGCTGG TTCCTTTAGT GAATGGTGTC
    AGAGAATTTT CAGAAATTCA GCTTGCTCGG CTAAAAAAAC TGGGAATAAA TAAGACTGAT
    CCGAGCACAC TGACAGAAGA GGAAGTGAGT AAATTTGCCC GTCTCGACAT CGACCCATCT
    ACCATCACGT GGCAGAGAGT ATTGGATACA AATGACCGAT TTCTACGAAA AATAACCATC
    GGGCAGGGAA ACACAGAGAA GGGCCATTAC CGGCAGGCGC AGTTTGACAT CGCAGTGGCC
    AGCGAGATCA TGGCGGTGCT GGCCCTGACG GACAGCCTCG CAGACATGAA GGCACGGCTG
    GGAAGGATGG TGGTGGCCAG TGACAAAAGC GGGCAGCCTG TGACAGCAGA TGATTTGGGG
    GTGACAGGTG CTTTGACAGT TTTGATGAAA GATGCAATAA AACCAAACCT GATGCAGACC
    CTGGAAGGGA CACCTGTGTT CGTGCATGCG GGCCCTTTTG CTAACATTGC TCACGGCAAC
    TCTTCAGTGT TGGCTGATAA AATTGCCCTG AAACTGGTTG GTGAAGAAGG ATTTGTAGTG
    ACCGAAGCTG GCTTTGGTGC TGACATCGGA ATGGAGAAAT TCTTCAACAT CAAGTGCCGA
    GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG TTAGTGGCAA CGGTGCGAGC TCTGAAGATG
    CATGGAGGCG GGCCAAGTGT AACGGCTGGT GTTCCTCTTA AGAAAGAATA TACAGAGGAG
    AACATCCAGC TGGTGGCAGA CGGCTGCTGT AACCTCCAGA AGCAAATTCA GATCACTCAG
    CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG AATGTCTTCA AGACCGACAC CCGCGCTGAG
    ATTGACTTGG TGTGTGAGCT TGCAAAGCGG GCTGGTGCCT TTGATGCAGT CCCCTGCTAT
    CACTGGTCCG TTGGTGGAAA AGGATCGGTG GACTTGGCTC GGGCTGTGAG AGAGGCTGCG
    AGTAAAAGAA GCCGATTCCA GTTCCTGTAT GATGTTCAGG TTCCAATTGT GGACAAGATA
    AGGACCATTG CTCAGGCTGT CTATGGAGCC AAAGATATTG AACTCTCTCC TGAGGCACAA
    GCCAAAATAG ATCGTTACAC TCAACAGGGT TTTGGAAATT TGCCCATCTG CATGGCAAAG
    ACCCACCTTT CTCTATCTCA CCAACCTGAC AAAAAAGGTG TGCCAAGGGA CTTCATCTTA
    CCTATCAGTG ACGTCCGGGC CAGCATAGGC GCTGGGTTCA TTTACCCTTT GGTCGGAACG
    ATGAGCACCA TGCCAGGACT GCCCACCCGG CCCTGCTTTT ATGACATAGA TCTTGATACC
    GAAACAGAAC AAGTTAAAGG CTTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337421.1
    CDS1240..3849
    Translation

    Target ORF information:

    RefSeq Version NM_001350492.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350492.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGGAAA TCAACCAGAA TTTGGCTGAG GAGGCTGGTC TGAACATCAC TCACATTTGC 
    CTCCCTCCAG ATAGCAGTGA AGCCGAGATT ATAGATGAAA TCTTAAAGAT CAATGAAGAT
    ACCAGAGTAC ATGGCCTTGC CCTTCAGATC TCTGAGAACT TGTTTAGCAA CAAAGTCCTC
    AATGCCTTGA AACCAGAAAA AGATGTGGAT GGAGTAACAG ACATAAACCT GGGGAAGCTG
    GTGCGAGGGG ATGCCCATGA ATGTTTTGTT TCACCTGTTG CCAAAGCTGT AATTGAACTT
    CTTGAAAAAT CAGTAGGTGT CAACCTAGAT GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT
    GGGTCTTTGG AAGCTGCTCT ACAATGCCTG TTCCAGAGAA AAGGGTCCAT GACAATGAGC
    ATCCAGTGGA AAACACGCCA GCTTCAAAGC AAGCTTCACG AGGCTGACAT TGTGGTCCTA
    GGCTCACCTA AGCCAGAAGA GATTCCCCTT ACTTGGATAC AACCAGGAAC TACTGTTCTC
    AACTGCTCCC ATGACTTCCT GTCAGGGAAG GTTGGGTGTG GCTCTCCAAG AATACATTTT
    GGTGGACTCA TTGAGGAAGA TGATGTGATT CTCCTTGCTG CAGCTCTGCG AATTCAGAAC
    ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT GAACAGCAGC ACAGGCGGTG GAGACTTCAC
    TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG CCAAGTGACA TTGAGATTTC AAGAGGACAA
    ACTCCAAAAG CTGTGGATGT CCTTGCCAAG GAGATTGGAT TGCTTGCAGA TGAAATTGAA
    ATCTATGGCA AAAGCAAAGC CAAAGTACGT TTGTCCGTGC TAGAAAGGTT AAAGGATCAA
    GCAGATGGAA AATACGTCTT AGTTGCTGGG ATCACACCCA CCCCTCTTGG AGAAGGGAAG
    AGCACAGTCA CCATCGGGCT TGTGCAGGCT CTGACCGCAC ACCTGAATGT CAACTCCTTT
    GCCTGCTTGA GGCAGCCTTC CCAAGGACCG ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT
    GGTGGATATG CCCAGGTCAT CCCCATGGAG GAGTTCAACC TTCACTTGAC TGGAGACATC
    CACGCCATCA CCGCTGCCAA TAACTTGCTG GCTGCCGCCA TCGACACGAG GATTCTTCAT
    GAAAACACGC AAACAGATAA GGCTCTGTAT AATCGGCTGG TTCCTTTAGT GAATGGTGTC
    AGAGAATTTT CAGAAATTCA GCTTGCTCGG CTAAAAAAAC TGGGAATAAA TAAGACTGAT
    CCGAGCACAC TGACAGAAGA GGAAGTGAGT AAATTTGCCC GTCTCGACAT CGACCCATCT
    ACCATCACGT GGCAGAGAGT ATTGGATACA AATGACCGAT TTCTACGAAA AATAACCATC
    GGGCAGGGAA ACACAGAGAA GGGCCATTAC CGGCAGGCGC AGTTTGACAT CGCAGTGGCC
    AGCGAGATCA TGGCGGTGCT GGCCCTGACG GACAGCCTCG CAGACATGAA GGCACGGCTG
    GGAAGGATGG TGGTGGCCAG TGACAAAAGC GGGCAGCCTG TGACAGCAGA TGATTTGGGG
    GTGACAGGTG CTTTGACAGT TTTGATGAAA GATGCAATAA AACCAAACCT GATGCAGACC
    CTGGAAGGGA CACCTGTGTT CGTGCATGCG GGCCCTTTTG CTAACATTGC TCACGGCAAC
    TCTTCAGTGT TGGCTGATAA AATTGCCCTG AAACTGGTTG GTGAAGAAGG ATTTGTAGTG
    ACCGAAGCTG GCTTTGGTGC TGACATCGGA ATGGAGAAAT TCTTCAACAT CAAGTGCCGA
    GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG TTAGTGGCAA CGGTGCGAGC TCTGAAGATG
    CATGGAGGCG GGCCAAGTGT AACGGCTGGT GTTCCTCTTA AGAAAGAATA TACAGAGGAG
    AACATCCAGC TGGTGGCAGA CGGCTGCTGT AACCTCCAGA AGCAAATTCA GATCACTCAG
    CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG AATGTCTTCA AGACCGACAC CCGCGCTGAG
    ATTGACTTGG TGTGTGAGCT TGCAAAGCGG GCTGGTGCCT TTGATGCAGT CCCCTGCTAT
    CACTGGTCCG TTGGTGGAAA AGGATCGGTG GACTTGGCTC GGGCTGTGAG AGAGGCTGCG
    AGTAAAAGAA GCCGATTCCA GTTCCTGTAT GATGTTCAGG TTCCAATTGT GGACAAGATA
    AGGACCATTG CTCAGGCTGT CTATGGAGCC AAAGATATTG AACTCTCTCC TGAGGCACAA
    GCCAAAATAG ATCGTTACAC TCAACAGGGT TTTGGAAATT TGCCCATCTG CATGGCAAAG
    ACCCACCTTT CTCTATCTCA CCAACCTGAC AAAAAAGGTG TGCCAAGGGA CTTCATCTTA
    CCTATCAGTG ACGTCCGGGC CAGCATAGGC GCTGGGTTCA TTTACCCTTT GGTCGGAACG
    ATGAGCACCA TGCCAGGACT GCCCACCCGG CCCTGCTTTT ATGACATAGA TCTTGATACC
    GAAACAGAAC AAGTTAAAGG CTTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu07102
    Clone ID Related Accession (Same CDS sequence) NM_001242768.1
    Accession Version NM_001242768.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA195967.1, AK315378.1 and BC110319.1. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.120835.1, SRR1660807.38268.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAATCGC GCCGGGCGCG ACCCAGACGA GAAGTCATTC AGAATTCAAA AGAAGTTCTA 
    AGTTTATTGC AAGAAAAAAA CCCTGCCTTC AAGCCGGTTC TTGCAATTAT CCAGGCAGGT
    GACGACAACT TGATGCAGGA AATCAACCAG AATTTGGCTG AGGAGGCTGG TCTGAACATC
    ACTCACATTT GCCTCCCTCC AGATAGCAGT GAAGCCGAGA TTATAGATGA AATCTTAAAG
    ATCAATGAAG ATACCAGAGT ACATGGCCTT GCCCTTCAGA TCTCTGAGAA CTTGTTTAGC
    AACAAAGTCC TCAATGCCTT GAAACCAGAA AAAGATGTGG ATGGAGTAAC AGACATAAAC
    CTGGGGAAGC TGGTGCGAGG GGATGCCCAT GAATGTTTTG TTTCACCTGT TGCCAAAGCT
    GTAATTGAAC TTCTTGAAAA ATCAGTAGGT GTCAACCTAG ATGGAAAGAA GATTTTGGTA
    GTGGGGGCCC ATGGGTCTTT GGAAGCTGCT CTACAATGCC TGTTCCAGAG AAAAGGGTCC
    ATGACAATGA GCATCCAGTG GAAAACACGC CAGCTTCAAA GCAAGCTTCA CGAGGCTGAC
    ATTGTGGTCC TAGGCTCACC TAAGCCAGAA GAGATTCCCC TTACTTGGAT ACAACCAGGA
    ACTACTGTTC TCAACTGCTC CCATGACTTC CTGTCAGGGA AGGTTGGGTG TGGCTCTCCA
    AGAATACATT TTGGTGGACT CATTGAGGAA GATGATGTGA TTCTCCTTGC TGCAGCTCTG
    CGAATTCAGA ACATGGTCAG TAGTGGAAGG AGATGGCTTC GTGAACAGCA GCACAGGCGG
    TGGAGACTTC ACTGCTTGAA ACTTCAGCCT CTCTCCCCTG TGCCAAGTGA CATTGAGATT
    TCAAGAGGAC AAACTCCAAA AGCTGTGGAT GTCCTTGCCA AGGAGATTGG ATTGCTTGCA
    GATGAAATTG AAATCTATGG CAAAAGCAAA GCCAAAGTAC GTTTGTCCGT GCTAGAAAGG
    TTAAAGGATC AAGCAGATGG AAAATACGTC TTAGTTGCTG GGATCACACC CACCCCTCTT
    GGAGAAGGGA AGAGCACAGT CACCATCGGG CTTGTGCAGG CTCTGACCGC ACACCTGAAT
    GTCAACTCCT TTGCCTGCTT GAGGCAGCCT TCCCAAGGAC CGACGTTTGG AGTGAAAGGA
    GGAGCCGCGG GTGGTGGATA TGCCCAGGTC ATCCCCATGG AGGAGTTCAA CCTTCACTTG
    ACTGGAGACA TCCACGCCAT CACCGCTGCC AATAACTTGC TGGCTGCCGC CATCGACACG
    AGGATTCTTC ATGAAAACAC GCAAACAGAT AAGGCTCTGT ATAATCGGCT GGTTCCTTTA
    GTGAATGGTG TCAGAGAATT TTCAGAAATT CAGCTTGCTC GGCTAAAAAA ACTGGGAATA
    AATAAGACTG ATCCGAGCAC ACTGACAGAA GAGGAAGTGA GTAAATTTGC CCGTCTCGAC
    ATCGACCCAT CTACCATCAC GTGGCAGAGA GTATTGGATA CAAATGACCG ATTTCTACGA
    AAAATAACCA TCGGGCAGGG AAACACAGAG AAGGGCCATT ACCGGCAGGC GCAGTTTGAC
    ATCGCAGTGG CCAGCGAGAT CATGGCGGTG CTGGCCCTGA CGGACAGCCT CGCAGACATG
    AAGGCACGGC TGGGAAGGAT GGTGGTGGCC AGTGACAAAA GCGGGCAGCC TGTGACAGCA
    GATGATTTGG GGGTGACAGG TGCTTTGACA GTTTTGATGA AAGATGCAAT AAAACCAAAC
    CTGATGCAGA CCCTGGAAGG GACACCTGTG TTCGTGCATG CGGGCCCTTT TGCTAACATT
    GCTCACGGCA ACTCTTCAGT GTTGGCTGAT AAAATTGCCC TGAAACTGGT TGGTGAAGAA
    GGATTTGTAG TGACCGAAGC TGGCTTTGGT GCTGACATCG GAATGGAGAA ATTCTTCAAC
    ATCAAGTGCC GAGCTTCCGG CTTGGTGCCC AACGTGGTTG TGTTAGTGGC AACGGTGCGA
    GCTCTGAAGA TGCATGGAGG CGGGCCAAGT GTAACGGCTG GTGTTCCTCT TAAGAAAGAA
    TATACAGAGG AGAACATCCA GCTGGTGGCA GACGGCTGCT GTAACCTCCA GAAGCAAATT
    CAGATCACTC AGCTCTTTGG GGTTCCCGTT GTGGTGGCTC TGAATGTCTT CAAGACCGAC
    ACCCGCGCTG AGATTGACTT GGTGTGTGAG CTTGCAAAGC GGGCTGGTGC CTTTGATGCA
    GTCCCCTGCT ATCACTGGTC CGTTGGTGGA AAAGGATCGG TGGACTTGGC TCGGGCTGTG
    AGAGAGGCTG CGAGTAAAAG AAGCCGATTC CAGTTCCTGT ATGATGTTCA GGTTCCAATT
    GTGGACAAGA TAAGGACCAT TGCTCAGGCT GTCTATGGAG CCAAAGATAT TGAACTCTCT
    CCTGAGGCAC AAGCCAAAAT AGATCGTTAC ACTCAACAGG GTTTTGGAAA TTTGCCCATC
    TGCATGGCAA AGACCCACCT TTCTCTATCT CACCAACCTG ACAAAAAAGG TGTGCCAAGG
    GACTTCATCT TACCTATCAG TGACGTCCGG GCCAGCATAG GCGCTGGGTT CATTTACCCT
    TTGGTCGGAA CGATGAGCAC CATGCCAGGA CTGCCCACCC GGCCCTGCTT TTATGACATA
    GATCTTGATA CCGAAACAGA ACAAGTTAAA GGCTTGTTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001229697.1
    CDS27..2768
    Translation

    Target ORF information:

    RefSeq Version NM_001242768.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001242768.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAATCGC GCCGGGCGCG ACCCAGACGA GAAGTCATTC AGAATTCAAA AGAAGTTCTA 
    AGTTTATTGC AAGAAAAAAA CCCTGCCTTC AAGCCGGTTC TTGCAATTAT CCAGGCAGGT
    GACGACAACT TGATGCAGGA AATCAACCAG AATTTGGCTG AGGAGGCTGG TCTGAACATC
    ACTCACATTT GCCTCCCTCC AGATAGCAGT GAAGCCGAGA TTATAGATGA AATCTTAAAG
    ATCAATGAAG ATACCAGAGT ACATGGCCTT GCCCTTCAGA TCTCTGAGAA CTTGTTTAGC
    AACAAAGTCC TCAATGCCTT GAAACCAGAA AAAGATGTGG ATGGAGTAAC AGACATAAAC
    CTGGGGAAGC TGGTGCGAGG GGATGCCCAT GAATGTTTTG TTTCACCTGT TGCCAAAGCT
    GTAATTGAAC TTCTTGAAAA ATCAGTAGGT GTCAACCTAG ATGGAAAGAA GATTTTGGTA
    GTGGGGGCCC ATGGGTCTTT GGAAGCTGCT CTACAATGCC TGTTCCAGAG AAAAGGGTCC
    ATGACAATGA GCATCCAGTG GAAAACACGC CAGCTTCAAA GCAAGCTTCA CGAGGCTGAC
    ATTGTGGTCC TAGGCTCACC TAAGCCAGAA GAGATTCCCC TTACTTGGAT ACAACCAGGA
    ACTACTGTTC TCAACTGCTC CCATGACTTC CTGTCAGGGA AGGTTGGGTG TGGCTCTCCA
    AGAATACATT TTGGTGGACT CATTGAGGAA GATGATGTGA TTCTCCTTGC TGCAGCTCTG
    CGAATTCAGA ACATGGTCAG TAGTGGAAGG AGATGGCTTC GTGAACAGCA GCACAGGCGG
    TGGAGACTTC ACTGCTTGAA ACTTCAGCCT CTCTCCCCTG TGCCAAGTGA CATTGAGATT
    TCAAGAGGAC AAACTCCAAA AGCTGTGGAT GTCCTTGCCA AGGAGATTGG ATTGCTTGCA
    GATGAAATTG AAATCTATGG CAAAAGCAAA GCCAAAGTAC GTTTGTCCGT GCTAGAAAGG
    TTAAAGGATC AAGCAGATGG AAAATACGTC TTAGTTGCTG GGATCACACC CACCCCTCTT
    GGAGAAGGGA AGAGCACAGT CACCATCGGG CTTGTGCAGG CTCTGACCGC ACACCTGAAT
    GTCAACTCCT TTGCCTGCTT GAGGCAGCCT TCCCAAGGAC CGACGTTTGG AGTGAAAGGA
    GGAGCCGCGG GTGGTGGATA TGCCCAGGTC ATCCCCATGG AGGAGTTCAA CCTTCACTTG
    ACTGGAGACA TCCACGCCAT CACCGCTGCC AATAACTTGC TGGCTGCCGC CATCGACACG
    AGGATTCTTC ATGAAAACAC GCAAACAGAT AAGGCTCTGT ATAATCGGCT GGTTCCTTTA
    GTGAATGGTG TCAGAGAATT TTCAGAAATT CAGCTTGCTC GGCTAAAAAA ACTGGGAATA
    AATAAGACTG ATCCGAGCAC ACTGACAGAA GAGGAAGTGA GTAAATTTGC CCGTCTCGAC
    ATCGACCCAT CTACCATCAC GTGGCAGAGA GTATTGGATA CAAATGACCG ATTTCTACGA
    AAAATAACCA TCGGGCAGGG AAACACAGAG AAGGGCCATT ACCGGCAGGC GCAGTTTGAC
    ATCGCAGTGG CCAGCGAGAT CATGGCGGTG CTGGCCCTGA CGGACAGCCT CGCAGACATG
    AAGGCACGGC TGGGAAGGAT GGTGGTGGCC AGTGACAAAA GCGGGCAGCC TGTGACAGCA
    GATGATTTGG GGGTGACAGG TGCTTTGACA GTTTTGATGA AAGATGCAAT AAAACCAAAC
    CTGATGCAGA CCCTGGAAGG GACACCTGTG TTCGTGCATG CGGGCCCTTT TGCTAACATT
    GCTCACGGCA ACTCTTCAGT GTTGGCTGAT AAAATTGCCC TGAAACTGGT TGGTGAAGAA
    GGATTTGTAG TGACCGAAGC TGGCTTTGGT GCTGACATCG GAATGGAGAA ATTCTTCAAC
    ATCAAGTGCC GAGCTTCCGG CTTGGTGCCC AACGTGGTTG TGTTAGTGGC AACGGTGCGA
    GCTCTGAAGA TGCATGGAGG CGGGCCAAGT GTAACGGCTG GTGTTCCTCT TAAGAAAGAA
    TATACAGAGG AGAACATCCA GCTGGTGGCA GACGGCTGCT GTAACCTCCA GAAGCAAATT
    CAGATCACTC AGCTCTTTGG GGTTCCCGTT GTGGTGGCTC TGAATGTCTT CAAGACCGAC
    ACCCGCGCTG AGATTGACTT GGTGTGTGAG CTTGCAAAGC GGGCTGGTGC CTTTGATGCA
    GTCCCCTGCT ATCACTGGTC CGTTGGTGGA AAAGGATCGG TGGACTTGGC TCGGGCTGTG
    AGAGAGGCTG CGAGTAAAAG AAGCCGATTC CAGTTCCTGT ATGATGTTCA GGTTCCAATT
    GTGGACAAGA TAAGGACCAT TGCTCAGGCT GTCTATGGAG CCAAAGATAT TGAACTCTCT
    CCTGAGGCAC AAGCCAAAAT AGATCGTTAC ACTCAACAGG GTTTTGGAAA TTTGCCCATC
    TGCATGGCAA AGACCCACCT TTCTCTATCT CACCAACCTG ACAAAAAAGG TGTGCCAAGG
    GACTTCATCT TACCTATCAG TGACGTCCGG GCCAGCATAG GCGCTGGGTT CATTTACCCT
    TTGGTCGGAA CGATGAGCAC CATGCCAGGA CTGCCCACCC GGCCCTGCTT TTATGACATA
    GATCTTGATA CCGAAACAGA ACAAGTTAAA GGCTTGTTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66609
    Clone ID Related Accession (Same CDS sequence) XM_011535730.2
    Accession Version XM_011535730.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535730.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCAATCGC GCCGGGCGCG ACCCAGACGA GAAGTCATTC AGAATTCAAA AGAAGTTCTA 
    AGTTTATTGC AAGAAAAAAA CCCTGCCTTC AAGCCGGTTC TTGCAATTAT CCAGGCAGGT
    GACGACAACT TGATGCAGGA AATCAACCAG AATTTGGCTG AGGAGGCTGG TCTGAACATC
    ACTCACATTT GCCTCCCTCC AGATAGCAGT GAAGCCGAGA TTATAGATGA AATCTTAAAG
    ATCAATGAAG ATACCAGAGT ACATGGCCTT GCCCTTCAGA TCTCTGAGAA CTTGTTTAGC
    AACAAAGTCC TCAATGCCTT GAAACCAGAA AAAGATGTGG ATGGAGTAAC AGACATAAAC
    CTGGGGAAGC TGGTGCGAGG GGATGCCCAT GAATGTTTTG TTTCACCTGT TGCCAAAGCT
    GTAATTGAAC TTCTTGAAAA ATCAGTAGGT GTCAACCTAG ATGGAAAGAA GATTTTGGTA
    GTGGGGGCCC ATGGGTCTTT GGAAGCTGCT CTACAATGCC TGTTCCAGAG AAAAGGGTCC
    ATGACAATGA GCATCCAGTG GAAAACACGC CAGCTTCAAA GCAAGCTTCA CGAGGCTGAC
    ATTGTGGTCC TAGGCTCACC TAAGCCAGAA GAGATTCCCC TTACTTGGAT ACAACCAGGA
    ACTACTGTTC TCAACTGCTC CCATGACTTC CTGTCAGGGA AGGTTGGGTG TGGCTCTCCA
    AGAATACATT TTGGTGGACT CATTGAGGAA GATGATGTGA TTCTCCTTGC TGCAGCTCTG
    CGAATTCAGA ACATGGTCAG TAGTGGAAGG AGATGGCTTC GTGAACAGCA GCACAGGCGG
    TGGAGACTTC ACTGCTTGAA ACTTCAGCCT CTCTCCCCTG TGCCAAGTGA CATTGAGATT
    TCAAGAGGAC AAACTCCAAA AGCTGTGGAT GTCCTTGCCA AGGAGATTGG ATTGCTTGCA
    GATGAAATTG AAATCTATGG CAAAAGCAAA GCCAAAGTAC GTTTGTCCGT GCTAGAAAGG
    TTAAAGGATC AAGCAGATGG AAAATACGTC TTAGTTGCTG GGATCACACC CACCCCTCTT
    GGAGAAGGGA AGAGCACAGT CACCATCGGG CTTGTGCAGG CTCTGACCGC ACACCTGAAT
    GTCAACTCCT TTGCCTGCTT GAGGCAGCCT TCCCAAGGAC CGACGTTTGG AGTGAAAGGA
    GGAGCCGCGG GTGGTGGATA TGCCCAGGTC ATCCCCATGG AGGAGTTCAA CCTTCACTTG
    ACTGGAGACA TCCACGCCAT CACCGCTGCC AATAACTTGC TGGCTGCCGC CATCGACACG
    AGGATTCTTC ATGAAAACAC GCAAACAGAT AAGGCTCTGT ATAATCGGCT GGTTCCTTTA
    GTGAATGGTG TCAGAGAATT TTCAGAAATT CAGCTTGCTC GGCTAAAAAA ACTGGGAATA
    AATAAGACTG ATCCGAGCAC ACTGACAGAA GAGGAAGTGA GTAAATTTGC CCGTCTCGAC
    ATCGACCCAT CTACCATCAC GTGGCAGAGA GTATTGGATA CAAATGACCG ATTTCTACGA
    AAAATAACCA TCGGGCAGGG AAACACAGAG AAGGGCCATT ACCGGCAGGC GCAGTTTGAC
    ATCGCAGTGG CCAGCGAGAT CATGGCGGTG CTGGCCCTGA CGGACAGCCT CGCAGACATG
    AAGGCACGGC TGGGAAGGAT GGTGGTGGCC AGTGACAAAA GCGGGCAGCC TGTGACAGCA
    GATGATTTGG GGGTGACAGG TGCTTTGACA GTTTTGATGA AAGATGCAAT AAAACCAAAC
    CTGATGCAGA CCCTGGAAGG GACACCTGTG TTCGTGCATG CGGGCCCTTT TGCTAACATT
    GCTCACGGCA ACTCTTCAGT GTTGGCTGAT AAAATTGCCC TGAAACTGGT TGGTGAAGAA
    GGATTTGTAG TGACCGAAGC TGGCTTTGGT GCTGACATCG GAATGGAGAA ATTCTTCAAC
    ATCAAGTGCC GAGCTTCCGG CTTGGTGCCC AACGTGGTTG TGTTAGTGGC AACGGTGCGA
    GCTCTGAAGA TGCATGGAGG CGGGCCAAGT GTAACGGCTG GTGTTCCTCT TAAGAAAGAA
    TATACAGAGG AGAACATCCA GCTGGTGGCA GACGGCTGCT GTAACCTCCA GAAGCAAATT
    CAGATCACTC AGCTCTTTGG GGTTCCCGTT GTGGTGGCTC TGAATGTCTT CAAGACCGAC
    ACCCGCGCTG AGATTGACTT GGTGTGTGAG CTTGCAAAGC GGGCTGGTGC CTTTGATGCA
    GTCCCCTGCT ATCACTGGTC CGTTGGTGGA AAAGGATCGG TGGACTTGGC TCGGGCTGTG
    AGAGAGGCTG CGAGTAAAAG AAGCCGATTC CAGTTCCTGT ATGATGTTCA GGTTCCAATT
    GTGGACAAGA TAAGGACCAT TGCTCAGGCT GTCTATGGAG CCAAAGATAT TGAACTCTCT
    CCTGAGGCAC AAGCCAAAAT AGATCGTTAC ACTCAACAGG GTTTTGGAAA TTTGCCCATC
    TGCATGGCAA AGACCCACCT TTCTCTATCT CACCAACCTG ACAAAAAAGG TGTGCCAAGG
    GACTTCATCT TACCTATCAG TGACGTCCGG GCCAGCATAG GCGCTGGGTT CATTTACCCT
    TTGGTCGGAA CGGCAAACCT GCTCATGTTC TTCGCTGAAA TCAACCTCCC CTTTCTGGAA
    TGGACATATG GAAAATTGGA GGCAAAATAT AATAATGGTG GTGACTCAGA CTGCTTTAAG
    GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011534032.1
    CDS27..2792
    Translation

    Target ORF information:

    RefSeq Version XM_011535730.2
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011535730.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCAATCGC GCCGGGCGCG ACCCAGACGA GAAGTCATTC AGAATTCAAA AGAAGTTCTA 
    AGTTTATTGC AAGAAAAAAA CCCTGCCTTC AAGCCGGTTC TTGCAATTAT CCAGGCAGGT
    GACGACAACT TGATGCAGGA AATCAACCAG AATTTGGCTG AGGAGGCTGG TCTGAACATC
    ACTCACATTT GCCTCCCTCC AGATAGCAGT GAAGCCGAGA TTATAGATGA AATCTTAAAG
    ATCAATGAAG ATACCAGAGT ACATGGCCTT GCCCTTCAGA TCTCTGAGAA CTTGTTTAGC
    AACAAAGTCC TCAATGCCTT GAAACCAGAA AAAGATGTGG ATGGAGTAAC AGACATAAAC
    CTGGGGAAGC TGGTGCGAGG GGATGCCCAT GAATGTTTTG TTTCACCTGT TGCCAAAGCT
    GTAATTGAAC TTCTTGAAAA ATCAGTAGGT GTCAACCTAG ATGGAAAGAA GATTTTGGTA
    GTGGGGGCCC ATGGGTCTTT GGAAGCTGCT CTACAATGCC TGTTCCAGAG AAAAGGGTCC
    ATGACAATGA GCATCCAGTG GAAAACACGC CAGCTTCAAA GCAAGCTTCA CGAGGCTGAC
    ATTGTGGTCC TAGGCTCACC TAAGCCAGAA GAGATTCCCC TTACTTGGAT ACAACCAGGA
    ACTACTGTTC TCAACTGCTC CCATGACTTC CTGTCAGGGA AGGTTGGGTG TGGCTCTCCA
    AGAATACATT TTGGTGGACT CATTGAGGAA GATGATGTGA TTCTCCTTGC TGCAGCTCTG
    CGAATTCAGA ACATGGTCAG TAGTGGAAGG AGATGGCTTC GTGAACAGCA GCACAGGCGG
    TGGAGACTTC ACTGCTTGAA ACTTCAGCCT CTCTCCCCTG TGCCAAGTGA CATTGAGATT
    TCAAGAGGAC AAACTCCAAA AGCTGTGGAT GTCCTTGCCA AGGAGATTGG ATTGCTTGCA
    GATGAAATTG AAATCTATGG CAAAAGCAAA GCCAAAGTAC GTTTGTCCGT GCTAGAAAGG
    TTAAAGGATC AAGCAGATGG AAAATACGTC TTAGTTGCTG GGATCACACC CACCCCTCTT
    GGAGAAGGGA AGAGCACAGT CACCATCGGG CTTGTGCAGG CTCTGACCGC ACACCTGAAT
    GTCAACTCCT TTGCCTGCTT GAGGCAGCCT TCCCAAGGAC CGACGTTTGG AGTGAAAGGA
    GGAGCCGCGG GTGGTGGATA TGCCCAGGTC ATCCCCATGG AGGAGTTCAA CCTTCACTTG
    ACTGGAGACA TCCACGCCAT CACCGCTGCC AATAACTTGC TGGCTGCCGC CATCGACACG
    AGGATTCTTC ATGAAAACAC GCAAACAGAT AAGGCTCTGT ATAATCGGCT GGTTCCTTTA
    GTGAATGGTG TCAGAGAATT TTCAGAAATT CAGCTTGCTC GGCTAAAAAA ACTGGGAATA
    AATAAGACTG ATCCGAGCAC ACTGACAGAA GAGGAAGTGA GTAAATTTGC CCGTCTCGAC
    ATCGACCCAT CTACCATCAC GTGGCAGAGA GTATTGGATA CAAATGACCG ATTTCTACGA
    AAAATAACCA TCGGGCAGGG AAACACAGAG AAGGGCCATT ACCGGCAGGC GCAGTTTGAC
    ATCGCAGTGG CCAGCGAGAT CATGGCGGTG CTGGCCCTGA CGGACAGCCT CGCAGACATG
    AAGGCACGGC TGGGAAGGAT GGTGGTGGCC AGTGACAAAA GCGGGCAGCC TGTGACAGCA
    GATGATTTGG GGGTGACAGG TGCTTTGACA GTTTTGATGA AAGATGCAAT AAAACCAAAC
    CTGATGCAGA CCCTGGAAGG GACACCTGTG TTCGTGCATG CGGGCCCTTT TGCTAACATT
    GCTCACGGCA ACTCTTCAGT GTTGGCTGAT AAAATTGCCC TGAAACTGGT TGGTGAAGAA
    GGATTTGTAG TGACCGAAGC TGGCTTTGGT GCTGACATCG GAATGGAGAA ATTCTTCAAC
    ATCAAGTGCC GAGCTTCCGG CTTGGTGCCC AACGTGGTTG TGTTAGTGGC AACGGTGCGA
    GCTCTGAAGA TGCATGGAGG CGGGCCAAGT GTAACGGCTG GTGTTCCTCT TAAGAAAGAA
    TATACAGAGG AGAACATCCA GCTGGTGGCA GACGGCTGCT GTAACCTCCA GAAGCAAATT
    CAGATCACTC AGCTCTTTGG GGTTCCCGTT GTGGTGGCTC TGAATGTCTT CAAGACCGAC
    ACCCGCGCTG AGATTGACTT GGTGTGTGAG CTTGCAAAGC GGGCTGGTGC CTTTGATGCA
    GTCCCCTGCT ATCACTGGTC CGTTGGTGGA AAAGGATCGG TGGACTTGGC TCGGGCTGTG
    AGAGAGGCTG CGAGTAAAAG AAGCCGATTC CAGTTCCTGT ATGATGTTCA GGTTCCAATT
    GTGGACAAGA TAAGGACCAT TGCTCAGGCT GTCTATGGAG CCAAAGATAT TGAACTCTCT
    CCTGAGGCAC AAGCCAAAAT AGATCGTTAC ACTCAACAGG GTTTTGGAAA TTTGCCCATC
    TGCATGGCAA AGACCCACCT TTCTCTATCT CACCAACCTG ACAAAAAAGG TGTGCCAAGG
    GACTTCATCT TACCTATCAG TGACGTCCGG GCCAGCATAG GCGCTGGGTT CATTTACCCT
    TTGGTCGGAA CGGCAAACCT GCTCATGTTC TTCGCTGAAA TCAACCTCCC CTTTCTGGAA
    TGGACATATG GAAAATTGGA GGCAAAATAT AATAATGGTG GTGACTCAGA CTGCTTTAAG
    GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66610
    Clone ID Related Accession (Same CDS sequence) XM_011535731.2
    Accession Version XM_011535731.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2763bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535731.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCAATCGC GCCGGGCGCG ACCCAGACGA GAAGTCATTC AGAATTCAAA AGAAGTTCTA 
    AGTTTATTGC AAGAAAAAAA CCCTGCCTTC AAGCCGGTTC TTGCAATTAT CCAGGCAGGT
    GACGACAACT TGATGCAGGA AATCAACCAG AATTTGGCTG AGGAGGCTGG TCTGAACATC
    ACTCACATTT GCCTCCCTCC AGATAGCAGT GAAGCCGAGA TTATAGATGA AATCTTAAAG
    ATCAATGAAG ATACCAGAGT ACATGGCCTT GCCCTTCAGA TCTCTGAGAA CTTGTTTAGC
    AACAAAGTCC TCAATGCCTT GAAACCAGAA AAAGATGTGG ATGGAGTAAC AGACATAAAC
    CTGGGGAAGC TGGTGCGAGG GGATGCCCAT GAATGTTTTG TTTCACCTGT TGCCAAAGCT
    GTAATTGAAC TTCTTGAAAA ATCAGGTGTC AACCTAGATG GAAAGAAGAT TTTGGTAGTG
    GGGGCCCATG GGTCTTTGGA AGCTGCTCTA CAATGCCTGT TCCAGAGAAA AGGGTCCATG
    ACAATGAGCA TCCAGTGGAA AACACGCCAG CTTCAAAGCA AGCTTCACGA GGCTGACATT
    GTGGTCCTAG GCTCACCTAA GCCAGAAGAG ATTCCCCTTA CTTGGATACA ACCAGGAACT
    ACTGTTCTCA ACTGCTCCCA TGACTTCCTG TCAGGGAAGG TTGGGTGTGG CTCTCCAAGA
    ATACATTTTG GTGGACTCAT TGAGGAAGAT GATGTGATTC TCCTTGCTGC AGCTCTGCGA
    ATTCAGAACA TGGTCAGTAG TGGAAGGAGA TGGCTTCGTG AACAGCAGCA CAGGCGGTGG
    AGACTTCACT GCTTGAAACT TCAGCCTCTC TCCCCTGTGC CAAGTGACAT TGAGATTTCA
    AGAGGACAAA CTCCAAAAGC TGTGGATGTC CTTGCCAAGG AGATTGGATT GCTTGCAGAT
    GAAATTGAAA TCTATGGCAA AAGCAAAGCC AAAGTACGTT TGTCCGTGCT AGAAAGGTTA
    AAGGATCAAG CAGATGGAAA ATACGTCTTA GTTGCTGGGA TCACACCCAC CCCTCTTGGA
    GAAGGGAAGA GCACAGTCAC CATCGGGCTT GTGCAGGCTC TGACCGCACA CCTGAATGTC
    AACTCCTTTG CCTGCTTGAG GCAGCCTTCC CAAGGACCGA CGTTTGGAGT GAAAGGAGGA
    GCCGCGGGTG GTGGATATGC CCAGGTCATC CCCATGGAGG AGTTCAACCT TCACTTGACT
    GGAGACATCC ACGCCATCAC CGCTGCCAAT AACTTGCTGG CTGCCGCCAT CGACACGAGG
    ATTCTTCATG AAAACACGCA AACAGATAAG GCTCTGTATA ATCGGCTGGT TCCTTTAGTG
    AATGGTGTCA GAGAATTTTC AGAAATTCAG CTTGCTCGGC TAAAAAAACT GGGAATAAAT
    AAGACTGATC CGAGCACACT GACAGAAGAG GAAGTGAGTA AATTTGCCCG TCTCGACATC
    GACCCATCTA CCATCACGTG GCAGAGAGTA TTGGATACAA ATGACCGATT TCTACGAAAA
    ATAACCATCG GGCAGGGAAA CACAGAGAAG GGCCATTACC GGCAGGCGCA GTTTGACATC
    GCAGTGGCCA GCGAGATCAT GGCGGTGCTG GCCCTGACGG ACAGCCTCGC AGACATGAAG
    GCACGGCTGG GAAGGATGGT GGTGGCCAGT GACAAAAGCG GGCAGCCTGT GACAGCAGAT
    GATTTGGGGG TGACAGGTGC TTTGACAGTT TTGATGAAAG ATGCAATAAA ACCAAACCTG
    ATGCAGACCC TGGAAGGGAC ACCTGTGTTC GTGCATGCGG GCCCTTTTGC TAACATTGCT
    CACGGCAACT CTTCAGTGTT GGCTGATAAA ATTGCCCTGA AACTGGTTGG TGAAGAAGGA
    TTTGTAGTGA CCGAAGCTGG CTTTGGTGCT GACATCGGAA TGGAGAAATT CTTCAACATC
    AAGTGCCGAG CTTCCGGCTT GGTGCCCAAC GTGGTTGTGT TAGTGGCAAC GGTGCGAGCT
    CTGAAGATGC ATGGAGGCGG GCCAAGTGTA ACGGCTGGTG TTCCTCTTAA GAAAGAATAT
    ACAGAGGAGA ACATCCAGCT GGTGGCAGAC GGCTGCTGTA ACCTCCAGAA GCAAATTCAG
    ATCACTCAGC TCTTTGGGGT TCCCGTTGTG GTGGCTCTGA ATGTCTTCAA GACCGACACC
    CGCGCTGAGA TTGACTTGGT GTGTGAGCTT GCAAAGCGGG CTGGTGCCTT TGATGCAGTC
    CCCTGCTATC ACTGGTCCGT TGGTGGAAAA GGATCGGTGG ACTTGGCTCG GGCTGTGAGA
    GAGGCTGCGA GTAAAAGAAG CCGATTCCAG TTCCTGTATG ATGTTCAGGT TCCAATTGTG
    GACAAGATAA GGACCATTGC TCAGGCTGTC TATGGAGCCA AAGATATTGA ACTCTCTCCT
    GAGGCACAAG CCAAAATAGA TCGTTACACT CAACAGGGTT TTGGAAATTT GCCCATCTGC
    ATGGCAAAGA CCCACCTTTC TCTATCTCAC CAACCTGACA AAAAAGGTGT GCCAAGGGAC
    TTCATCTTAC CTATCAGTGA CGTCCGGGCC AGCATAGGCG CTGGGTTCAT TTACCCTTTG
    GTCGGAACGG CAAACCTGCT CATGTTCTTC GCTGAAATCA ACCTCCCCTT TCTGGAATGG
    ACATATGGAA AATTGGAGGC AAAATATAAT AATGGTGGTG ACTCAGACTG CTTTAAGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011534033.1
    CDS2..2764
    Translation

    Target ORF information:

    RefSeq Version XM_011535731.2
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011535731.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCAATCGC GCCGGGCGCG ACCCAGACGA GAAGTCATTC AGAATTCAAA AGAAGTTCTA 
    AGTTTATTGC AAGAAAAAAA CCCTGCCTTC AAGCCGGTTC TTGCAATTAT CCAGGCAGGT
    GACGACAACT TGATGCAGGA AATCAACCAG AATTTGGCTG AGGAGGCTGG TCTGAACATC
    ACTCACATTT GCCTCCCTCC AGATAGCAGT GAAGCCGAGA TTATAGATGA AATCTTAAAG
    ATCAATGAAG ATACCAGAGT ACATGGCCTT GCCCTTCAGA TCTCTGAGAA CTTGTTTAGC
    AACAAAGTCC TCAATGCCTT GAAACCAGAA AAAGATGTGG ATGGAGTAAC AGACATAAAC
    CTGGGGAAGC TGGTGCGAGG GGATGCCCAT GAATGTTTTG TTTCACCTGT TGCCAAAGCT
    GTAATTGAAC TTCTTGAAAA ATCAGGTGTC AACCTAGATG GAAAGAAGAT TTTGGTAGTG
    GGGGCCCATG GGTCTTTGGA AGCTGCTCTA CAATGCCTGT TCCAGAGAAA AGGGTCCATG
    ACAATGAGCA TCCAGTGGAA AACACGCCAG CTTCAAAGCA AGCTTCACGA GGCTGACATT
    GTGGTCCTAG GCTCACCTAA GCCAGAAGAG ATTCCCCTTA CTTGGATACA ACCAGGAACT
    ACTGTTCTCA ACTGCTCCCA TGACTTCCTG TCAGGGAAGG TTGGGTGTGG CTCTCCAAGA
    ATACATTTTG GTGGACTCAT TGAGGAAGAT GATGTGATTC TCCTTGCTGC AGCTCTGCGA
    ATTCAGAACA TGGTCAGTAG TGGAAGGAGA TGGCTTCGTG AACAGCAGCA CAGGCGGTGG
    AGACTTCACT GCTTGAAACT TCAGCCTCTC TCCCCTGTGC CAAGTGACAT TGAGATTTCA
    AGAGGACAAA CTCCAAAAGC TGTGGATGTC CTTGCCAAGG AGATTGGATT GCTTGCAGAT
    GAAATTGAAA TCTATGGCAA AAGCAAAGCC AAAGTACGTT TGTCCGTGCT AGAAAGGTTA
    AAGGATCAAG CAGATGGAAA ATACGTCTTA GTTGCTGGGA TCACACCCAC CCCTCTTGGA
    GAAGGGAAGA GCACAGTCAC CATCGGGCTT GTGCAGGCTC TGACCGCACA CCTGAATGTC
    AACTCCTTTG CCTGCTTGAG GCAGCCTTCC CAAGGACCGA CGTTTGGAGT GAAAGGAGGA
    GCCGCGGGTG GTGGATATGC CCAGGTCATC CCCATGGAGG AGTTCAACCT TCACTTGACT
    GGAGACATCC ACGCCATCAC CGCTGCCAAT AACTTGCTGG CTGCCGCCAT CGACACGAGG
    ATTCTTCATG AAAACACGCA AACAGATAAG GCTCTGTATA ATCGGCTGGT TCCTTTAGTG
    AATGGTGTCA GAGAATTTTC AGAAATTCAG CTTGCTCGGC TAAAAAAACT GGGAATAAAT
    AAGACTGATC CGAGCACACT GACAGAAGAG GAAGTGAGTA AATTTGCCCG TCTCGACATC
    GACCCATCTA CCATCACGTG GCAGAGAGTA TTGGATACAA ATGACCGATT TCTACGAAAA
    ATAACCATCG GGCAGGGAAA CACAGAGAAG GGCCATTACC GGCAGGCGCA GTTTGACATC
    GCAGTGGCCA GCGAGATCAT GGCGGTGCTG GCCCTGACGG ACAGCCTCGC AGACATGAAG
    GCACGGCTGG GAAGGATGGT GGTGGCCAGT GACAAAAGCG GGCAGCCTGT GACAGCAGAT
    GATTTGGGGG TGACAGGTGC TTTGACAGTT TTGATGAAAG ATGCAATAAA ACCAAACCTG
    ATGCAGACCC TGGAAGGGAC ACCTGTGTTC GTGCATGCGG GCCCTTTTGC TAACATTGCT
    CACGGCAACT CTTCAGTGTT GGCTGATAAA ATTGCCCTGA AACTGGTTGG TGAAGAAGGA
    TTTGTAGTGA CCGAAGCTGG CTTTGGTGCT GACATCGGAA TGGAGAAATT CTTCAACATC
    AAGTGCCGAG CTTCCGGCTT GGTGCCCAAC GTGGTTGTGT TAGTGGCAAC GGTGCGAGCT
    CTGAAGATGC ATGGAGGCGG GCCAAGTGTA ACGGCTGGTG TTCCTCTTAA GAAAGAATAT
    ACAGAGGAGA ACATCCAGCT GGTGGCAGAC GGCTGCTGTA ACCTCCAGAA GCAAATTCAG
    ATCACTCAGC TCTTTGGGGT TCCCGTTGTG GTGGCTCTGA ATGTCTTCAA GACCGACACC
    CGCGCTGAGA TTGACTTGGT GTGTGAGCTT GCAAAGCGGG CTGGTGCCTT TGATGCAGTC
    CCCTGCTATC ACTGGTCCGT TGGTGGAAAA GGATCGGTGG ACTTGGCTCG GGCTGTGAGA
    GAGGCTGCGA GTAAAAGAAG CCGATTCCAG TTCCTGTATG ATGTTCAGGT TCCAATTGTG
    GACAAGATAA GGACCATTGC TCAGGCTGTC TATGGAGCCA AAGATATTGA ACTCTCTCCT
    GAGGCACAAG CCAAAATAGA TCGTTACACT CAACAGGGTT TTGGAAATTT GCCCATCTGC
    ATGGCAAAGA CCCACCTTTC TCTATCTCAC CAACCTGACA AAAAAGGTGT GCCAAGGGAC
    TTCATCTTAC CTATCAGTGA CGTCCGGGCC AGCATAGGCG CTGGGTTCAT TTACCCTTTG
    GTCGGAACGG CAAACCTGCT CATGTTCTTC GCTGAAATCA ACCTCCCCTT TCTGGAATGG
    ACATATGGAA AATTGGAGGC AAAATATAAT AATGGTGGTG ACTCAGACTG CTTTAAGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66611
    Clone ID Related Accession (Same CDS sequence) XM_011535732.2 , XM_011535733.2
    Accession Version XM_011535732.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2634bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535732.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGGAAA TCAACCAGAA TTTGGCTGAG GAGGCTGGTC TGAACATCAC TCACATTTGC 
    CTCCCTCCAG ATAGCAGTGA AGCCGAGATT ATAGATGAAA TCTTAAAGAT CAATGAAGAT
    ACCAGAGTAC ATGGCCTTGC CCTTCAGATC TCTGAGAACT TGTTTAGCAA CAAAGTCCTC
    AATGCCTTGA AACCAGAAAA AGATGTGGAT GGAGTAACAG ACATAAACCT GGGGAAGCTG
    GTGCGAGGGG ATGCCCATGA ATGTTTTGTT TCACCTGTTG CCAAAGCTGT AATTGAACTT
    CTTGAAAAAT CAGTAGGTGT CAACCTAGAT GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT
    GGGTCTTTGG AAGCTGCTCT ACAATGCCTG TTCCAGAGAA AAGGGTCCAT GACAATGAGC
    ATCCAGTGGA AAACACGCCA GCTTCAAAGC AAGCTTCACG AGGCTGACAT TGTGGTCCTA
    GGCTCACCTA AGCCAGAAGA GATTCCCCTT ACTTGGATAC AACCAGGAAC TACTGTTCTC
    AACTGCTCCC ATGACTTCCT GTCAGGGAAG GTTGGGTGTG GCTCTCCAAG AATACATTTT
    GGTGGACTCA TTGAGGAAGA TGATGTGATT CTCCTTGCTG CAGCTCTGCG AATTCAGAAC
    ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT GAACAGCAGC ACAGGCGGTG GAGACTTCAC
    TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG CCAAGTGACA TTGAGATTTC AAGAGGACAA
    ACTCCAAAAG CTGTGGATGT CCTTGCCAAG GAGATTGGAT TGCTTGCAGA TGAAATTGAA
    ATCTATGGCA AAAGCAAAGC CAAAGTACGT TTGTCCGTGC TAGAAAGGTT AAAGGATCAA
    GCAGATGGAA AATACGTCTT AGTTGCTGGG ATCACACCCA CCCCTCTTGG AGAAGGGAAG
    AGCACAGTCA CCATCGGGCT TGTGCAGGCT CTGACCGCAC ACCTGAATGT CAACTCCTTT
    GCCTGCTTGA GGCAGCCTTC CCAAGGACCG ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT
    GGTGGATATG CCCAGGTCAT CCCCATGGAG GAGTTCAACC TTCACTTGAC TGGAGACATC
    CACGCCATCA CCGCTGCCAA TAACTTGCTG GCTGCCGCCA TCGACACGAG GATTCTTCAT
    GAAAACACGC AAACAGATAA GGCTCTGTAT AATCGGCTGG TTCCTTTAGT GAATGGTGTC
    AGAGAATTTT CAGAAATTCA GCTTGCTCGG CTAAAAAAAC TGGGAATAAA TAAGACTGAT
    CCGAGCACAC TGACAGAAGA GGAAGTGAGT AAATTTGCCC GTCTCGACAT CGACCCATCT
    ACCATCACGT GGCAGAGAGT ATTGGATACA AATGACCGAT TTCTACGAAA AATAACCATC
    GGGCAGGGAA ACACAGAGAA GGGCCATTAC CGGCAGGCGC AGTTTGACAT CGCAGTGGCC
    AGCGAGATCA TGGCGGTGCT GGCCCTGACG GACAGCCTCG CAGACATGAA GGCACGGCTG
    GGAAGGATGG TGGTGGCCAG TGACAAAAGC GGGCAGCCTG TGACAGCAGA TGATTTGGGG
    GTGACAGGTG CTTTGACAGT TTTGATGAAA GATGCAATAA AACCAAACCT GATGCAGACC
    CTGGAAGGGA CACCTGTGTT CGTGCATGCG GGCCCTTTTG CTAACATTGC TCACGGCAAC
    TCTTCAGTGT TGGCTGATAA AATTGCCCTG AAACTGGTTG GTGAAGAAGG ATTTGTAGTG
    ACCGAAGCTG GCTTTGGTGC TGACATCGGA ATGGAGAAAT TCTTCAACAT CAAGTGCCGA
    GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG TTAGTGGCAA CGGTGCGAGC TCTGAAGATG
    CATGGAGGCG GGCCAAGTGT AACGGCTGGT GTTCCTCTTA AGAAAGAATA TACAGAGGAG
    AACATCCAGC TGGTGGCAGA CGGCTGCTGT AACCTCCAGA AGCAAATTCA GATCACTCAG
    CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG AATGTCTTCA AGACCGACAC CCGCGCTGAG
    ATTGACTTGG TGTGTGAGCT TGCAAAGCGG GCTGGTGCCT TTGATGCAGT CCCCTGCTAT
    CACTGGTCCG TTGGTGGAAA AGGATCGGTG GACTTGGCTC GGGCTGTGAG AGAGGCTGCG
    AGTAAAAGAA GCCGATTCCA GTTCCTGTAT GATGTTCAGG TTCCAATTGT GGACAAGATA
    AGGACCATTG CTCAGGCTGT CTATGGAGCC AAAGATATTG AACTCTCTCC TGAGGCACAA
    GCCAAAATAG ATCGTTACAC TCAACAGGGT TTTGGAAATT TGCCCATCTG CATGGCAAAG
    ACCCACCTTT CTCTATCTCA CCAACCTGAC AAAAAAGGTG TGCCAAGGGA CTTCATCTTA
    CCTATCAGTG ACGTCCGGGC CAGCATAGGC GCTGGGTTCA TTTACCCTTT GGTCGGAACG
    GCAAACCTGC TCATGTTCTT CGCTGAAATC AACCTCCCCT TTCTGGAATG GACATATGGA
    AAATTGGAGG CAAAATATAA TAATGGTGGT GACTCAGACT GCTTTAAGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011534034.1
    CDS213..2846
    Translation

    Target ORF information:

    RefSeq Version XM_011535732.2
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011535732.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGGAAA TCAACCAGAA TTTGGCTGAG GAGGCTGGTC TGAACATCAC TCACATTTGC 
    CTCCCTCCAG ATAGCAGTGA AGCCGAGATT ATAGATGAAA TCTTAAAGAT CAATGAAGAT
    ACCAGAGTAC ATGGCCTTGC CCTTCAGATC TCTGAGAACT TGTTTAGCAA CAAAGTCCTC
    AATGCCTTGA AACCAGAAAA AGATGTGGAT GGAGTAACAG ACATAAACCT GGGGAAGCTG
    GTGCGAGGGG ATGCCCATGA ATGTTTTGTT TCACCTGTTG CCAAAGCTGT AATTGAACTT
    CTTGAAAAAT CAGTAGGTGT CAACCTAGAT GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT
    GGGTCTTTGG AAGCTGCTCT ACAATGCCTG TTCCAGAGAA AAGGGTCCAT GACAATGAGC
    ATCCAGTGGA AAACACGCCA GCTTCAAAGC AAGCTTCACG AGGCTGACAT TGTGGTCCTA
    GGCTCACCTA AGCCAGAAGA GATTCCCCTT ACTTGGATAC AACCAGGAAC TACTGTTCTC
    AACTGCTCCC ATGACTTCCT GTCAGGGAAG GTTGGGTGTG GCTCTCCAAG AATACATTTT
    GGTGGACTCA TTGAGGAAGA TGATGTGATT CTCCTTGCTG CAGCTCTGCG AATTCAGAAC
    ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT GAACAGCAGC ACAGGCGGTG GAGACTTCAC
    TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG CCAAGTGACA TTGAGATTTC AAGAGGACAA
    ACTCCAAAAG CTGTGGATGT CCTTGCCAAG GAGATTGGAT TGCTTGCAGA TGAAATTGAA
    ATCTATGGCA AAAGCAAAGC CAAAGTACGT TTGTCCGTGC TAGAAAGGTT AAAGGATCAA
    GCAGATGGAA AATACGTCTT AGTTGCTGGG ATCACACCCA CCCCTCTTGG AGAAGGGAAG
    AGCACAGTCA CCATCGGGCT TGTGCAGGCT CTGACCGCAC ACCTGAATGT CAACTCCTTT
    GCCTGCTTGA GGCAGCCTTC CCAAGGACCG ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT
    GGTGGATATG CCCAGGTCAT CCCCATGGAG GAGTTCAACC TTCACTTGAC TGGAGACATC
    CACGCCATCA CCGCTGCCAA TAACTTGCTG GCTGCCGCCA TCGACACGAG GATTCTTCAT
    GAAAACACGC AAACAGATAA GGCTCTGTAT AATCGGCTGG TTCCTTTAGT GAATGGTGTC
    AGAGAATTTT CAGAAATTCA GCTTGCTCGG CTAAAAAAAC TGGGAATAAA TAAGACTGAT
    CCGAGCACAC TGACAGAAGA GGAAGTGAGT AAATTTGCCC GTCTCGACAT CGACCCATCT
    ACCATCACGT GGCAGAGAGT ATTGGATACA AATGACCGAT TTCTACGAAA AATAACCATC
    GGGCAGGGAA ACACAGAGAA GGGCCATTAC CGGCAGGCGC AGTTTGACAT CGCAGTGGCC
    AGCGAGATCA TGGCGGTGCT GGCCCTGACG GACAGCCTCG CAGACATGAA GGCACGGCTG
    GGAAGGATGG TGGTGGCCAG TGACAAAAGC GGGCAGCCTG TGACAGCAGA TGATTTGGGG
    GTGACAGGTG CTTTGACAGT TTTGATGAAA GATGCAATAA AACCAAACCT GATGCAGACC
    CTGGAAGGGA CACCTGTGTT CGTGCATGCG GGCCCTTTTG CTAACATTGC TCACGGCAAC
    TCTTCAGTGT TGGCTGATAA AATTGCCCTG AAACTGGTTG GTGAAGAAGG ATTTGTAGTG
    ACCGAAGCTG GCTTTGGTGC TGACATCGGA ATGGAGAAAT TCTTCAACAT CAAGTGCCGA
    GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG TTAGTGGCAA CGGTGCGAGC TCTGAAGATG
    CATGGAGGCG GGCCAAGTGT AACGGCTGGT GTTCCTCTTA AGAAAGAATA TACAGAGGAG
    AACATCCAGC TGGTGGCAGA CGGCTGCTGT AACCTCCAGA AGCAAATTCA GATCACTCAG
    CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG AATGTCTTCA AGACCGACAC CCGCGCTGAG
    ATTGACTTGG TGTGTGAGCT TGCAAAGCGG GCTGGTGCCT TTGATGCAGT CCCCTGCTAT
    CACTGGTCCG TTGGTGGAAA AGGATCGGTG GACTTGGCTC GGGCTGTGAG AGAGGCTGCG
    AGTAAAAGAA GCCGATTCCA GTTCCTGTAT GATGTTCAGG TTCCAATTGT GGACAAGATA
    AGGACCATTG CTCAGGCTGT CTATGGAGCC AAAGATATTG AACTCTCTCC TGAGGCACAA
    GCCAAAATAG ATCGTTACAC TCAACAGGGT TTTGGAAATT TGCCCATCTG CATGGCAAAG
    ACCCACCTTT CTCTATCTCA CCAACCTGAC AAAAAAGGTG TGCCAAGGGA CTTCATCTTA
    CCTATCAGTG ACGTCCGGGC CAGCATAGGC GCTGGGTTCA TTTACCCTTT GGTCGGAACG
    GCAAACCTGC TCATGTTCTT CGCTGAAATC AACCTCCCCT TTCTGGAATG GACATATGGA
    AAATTGGAGG CAAAATATAA TAATGGTGGT GACTCAGACT GCTTTAAGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66611
    Clone ID Related Accession (Same CDS sequence) XM_011535732.2 , XM_011535733.2
    Accession Version XM_011535733.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2634bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535733.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGGAAA TCAACCAGAA TTTGGCTGAG GAGGCTGGTC TGAACATCAC TCACATTTGC 
    CTCCCTCCAG ATAGCAGTGA AGCCGAGATT ATAGATGAAA TCTTAAAGAT CAATGAAGAT
    ACCAGAGTAC ATGGCCTTGC CCTTCAGATC TCTGAGAACT TGTTTAGCAA CAAAGTCCTC
    AATGCCTTGA AACCAGAAAA AGATGTGGAT GGAGTAACAG ACATAAACCT GGGGAAGCTG
    GTGCGAGGGG ATGCCCATGA ATGTTTTGTT TCACCTGTTG CCAAAGCTGT AATTGAACTT
    CTTGAAAAAT CAGTAGGTGT CAACCTAGAT GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT
    GGGTCTTTGG AAGCTGCTCT ACAATGCCTG TTCCAGAGAA AAGGGTCCAT GACAATGAGC
    ATCCAGTGGA AAACACGCCA GCTTCAAAGC AAGCTTCACG AGGCTGACAT TGTGGTCCTA
    GGCTCACCTA AGCCAGAAGA GATTCCCCTT ACTTGGATAC AACCAGGAAC TACTGTTCTC
    AACTGCTCCC ATGACTTCCT GTCAGGGAAG GTTGGGTGTG GCTCTCCAAG AATACATTTT
    GGTGGACTCA TTGAGGAAGA TGATGTGATT CTCCTTGCTG CAGCTCTGCG AATTCAGAAC
    ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT GAACAGCAGC ACAGGCGGTG GAGACTTCAC
    TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG CCAAGTGACA TTGAGATTTC AAGAGGACAA
    ACTCCAAAAG CTGTGGATGT CCTTGCCAAG GAGATTGGAT TGCTTGCAGA TGAAATTGAA
    ATCTATGGCA AAAGCAAAGC CAAAGTACGT TTGTCCGTGC TAGAAAGGTT AAAGGATCAA
    GCAGATGGAA AATACGTCTT AGTTGCTGGG ATCACACCCA CCCCTCTTGG AGAAGGGAAG
    AGCACAGTCA CCATCGGGCT TGTGCAGGCT CTGACCGCAC ACCTGAATGT CAACTCCTTT
    GCCTGCTTGA GGCAGCCTTC CCAAGGACCG ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT
    GGTGGATATG CCCAGGTCAT CCCCATGGAG GAGTTCAACC TTCACTTGAC TGGAGACATC
    CACGCCATCA CCGCTGCCAA TAACTTGCTG GCTGCCGCCA TCGACACGAG GATTCTTCAT
    GAAAACACGC AAACAGATAA GGCTCTGTAT AATCGGCTGG TTCCTTTAGT GAATGGTGTC
    AGAGAATTTT CAGAAATTCA GCTTGCTCGG CTAAAAAAAC TGGGAATAAA TAAGACTGAT
    CCGAGCACAC TGACAGAAGA GGAAGTGAGT AAATTTGCCC GTCTCGACAT CGACCCATCT
    ACCATCACGT GGCAGAGAGT ATTGGATACA AATGACCGAT TTCTACGAAA AATAACCATC
    GGGCAGGGAA ACACAGAGAA GGGCCATTAC CGGCAGGCGC AGTTTGACAT CGCAGTGGCC
    AGCGAGATCA TGGCGGTGCT GGCCCTGACG GACAGCCTCG CAGACATGAA GGCACGGCTG
    GGAAGGATGG TGGTGGCCAG TGACAAAAGC GGGCAGCCTG TGACAGCAGA TGATTTGGGG
    GTGACAGGTG CTTTGACAGT TTTGATGAAA GATGCAATAA AACCAAACCT GATGCAGACC
    CTGGAAGGGA CACCTGTGTT CGTGCATGCG GGCCCTTTTG CTAACATTGC TCACGGCAAC
    TCTTCAGTGT TGGCTGATAA AATTGCCCTG AAACTGGTTG GTGAAGAAGG ATTTGTAGTG
    ACCGAAGCTG GCTTTGGTGC TGACATCGGA ATGGAGAAAT TCTTCAACAT CAAGTGCCGA
    GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG TTAGTGGCAA CGGTGCGAGC TCTGAAGATG
    CATGGAGGCG GGCCAAGTGT AACGGCTGGT GTTCCTCTTA AGAAAGAATA TACAGAGGAG
    AACATCCAGC TGGTGGCAGA CGGCTGCTGT AACCTCCAGA AGCAAATTCA GATCACTCAG
    CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG AATGTCTTCA AGACCGACAC CCGCGCTGAG
    ATTGACTTGG TGTGTGAGCT TGCAAAGCGG GCTGGTGCCT TTGATGCAGT CCCCTGCTAT
    CACTGGTCCG TTGGTGGAAA AGGATCGGTG GACTTGGCTC GGGCTGTGAG AGAGGCTGCG
    AGTAAAAGAA GCCGATTCCA GTTCCTGTAT GATGTTCAGG TTCCAATTGT GGACAAGATA
    AGGACCATTG CTCAGGCTGT CTATGGAGCC AAAGATATTG AACTCTCTCC TGAGGCACAA
    GCCAAAATAG ATCGTTACAC TCAACAGGGT TTTGGAAATT TGCCCATCTG CATGGCAAAG
    ACCCACCTTT CTCTATCTCA CCAACCTGAC AAAAAAGGTG TGCCAAGGGA CTTCATCTTA
    CCTATCAGTG ACGTCCGGGC CAGCATAGGC GCTGGGTTCA TTTACCCTTT GGTCGGAACG
    GCAAACCTGC TCATGTTCTT CGCTGAAATC AACCTCCCCT TTCTGGAATG GACATATGGA
    AAATTGGAGG CAAAATATAA TAATGGTGGT GACTCAGACT GCTTTAAGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011534035.1
    CDS659..3292
    Translation

    Target ORF information:

    RefSeq Version XM_011535733.2
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011535733.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGGAAA TCAACCAGAA TTTGGCTGAG GAGGCTGGTC TGAACATCAC TCACATTTGC 
    CTCCCTCCAG ATAGCAGTGA AGCCGAGATT ATAGATGAAA TCTTAAAGAT CAATGAAGAT
    ACCAGAGTAC ATGGCCTTGC CCTTCAGATC TCTGAGAACT TGTTTAGCAA CAAAGTCCTC
    AATGCCTTGA AACCAGAAAA AGATGTGGAT GGAGTAACAG ACATAAACCT GGGGAAGCTG
    GTGCGAGGGG ATGCCCATGA ATGTTTTGTT TCACCTGTTG CCAAAGCTGT AATTGAACTT
    CTTGAAAAAT CAGTAGGTGT CAACCTAGAT GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT
    GGGTCTTTGG AAGCTGCTCT ACAATGCCTG TTCCAGAGAA AAGGGTCCAT GACAATGAGC
    ATCCAGTGGA AAACACGCCA GCTTCAAAGC AAGCTTCACG AGGCTGACAT TGTGGTCCTA
    GGCTCACCTA AGCCAGAAGA GATTCCCCTT ACTTGGATAC AACCAGGAAC TACTGTTCTC
    AACTGCTCCC ATGACTTCCT GTCAGGGAAG GTTGGGTGTG GCTCTCCAAG AATACATTTT
    GGTGGACTCA TTGAGGAAGA TGATGTGATT CTCCTTGCTG CAGCTCTGCG AATTCAGAAC
    ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT GAACAGCAGC ACAGGCGGTG GAGACTTCAC
    TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG CCAAGTGACA TTGAGATTTC AAGAGGACAA
    ACTCCAAAAG CTGTGGATGT CCTTGCCAAG GAGATTGGAT TGCTTGCAGA TGAAATTGAA
    ATCTATGGCA AAAGCAAAGC CAAAGTACGT TTGTCCGTGC TAGAAAGGTT AAAGGATCAA
    GCAGATGGAA AATACGTCTT AGTTGCTGGG ATCACACCCA CCCCTCTTGG AGAAGGGAAG
    AGCACAGTCA CCATCGGGCT TGTGCAGGCT CTGACCGCAC ACCTGAATGT CAACTCCTTT
    GCCTGCTTGA GGCAGCCTTC CCAAGGACCG ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT
    GGTGGATATG CCCAGGTCAT CCCCATGGAG GAGTTCAACC TTCACTTGAC TGGAGACATC
    CACGCCATCA CCGCTGCCAA TAACTTGCTG GCTGCCGCCA TCGACACGAG GATTCTTCAT
    GAAAACACGC AAACAGATAA GGCTCTGTAT AATCGGCTGG TTCCTTTAGT GAATGGTGTC
    AGAGAATTTT CAGAAATTCA GCTTGCTCGG CTAAAAAAAC TGGGAATAAA TAAGACTGAT
    CCGAGCACAC TGACAGAAGA GGAAGTGAGT AAATTTGCCC GTCTCGACAT CGACCCATCT
    ACCATCACGT GGCAGAGAGT ATTGGATACA AATGACCGAT TTCTACGAAA AATAACCATC
    GGGCAGGGAA ACACAGAGAA GGGCCATTAC CGGCAGGCGC AGTTTGACAT CGCAGTGGCC
    AGCGAGATCA TGGCGGTGCT GGCCCTGACG GACAGCCTCG CAGACATGAA GGCACGGCTG
    GGAAGGATGG TGGTGGCCAG TGACAAAAGC GGGCAGCCTG TGACAGCAGA TGATTTGGGG
    GTGACAGGTG CTTTGACAGT TTTGATGAAA GATGCAATAA AACCAAACCT GATGCAGACC
    CTGGAAGGGA CACCTGTGTT CGTGCATGCG GGCCCTTTTG CTAACATTGC TCACGGCAAC
    TCTTCAGTGT TGGCTGATAA AATTGCCCTG AAACTGGTTG GTGAAGAAGG ATTTGTAGTG
    ACCGAAGCTG GCTTTGGTGC TGACATCGGA ATGGAGAAAT TCTTCAACAT CAAGTGCCGA
    GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG TTAGTGGCAA CGGTGCGAGC TCTGAAGATG
    CATGGAGGCG GGCCAAGTGT AACGGCTGGT GTTCCTCTTA AGAAAGAATA TACAGAGGAG
    AACATCCAGC TGGTGGCAGA CGGCTGCTGT AACCTCCAGA AGCAAATTCA GATCACTCAG
    CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG AATGTCTTCA AGACCGACAC CCGCGCTGAG
    ATTGACTTGG TGTGTGAGCT TGCAAAGCGG GCTGGTGCCT TTGATGCAGT CCCCTGCTAT
    CACTGGTCCG TTGGTGGAAA AGGATCGGTG GACTTGGCTC GGGCTGTGAG AGAGGCTGCG
    AGTAAAAGAA GCCGATTCCA GTTCCTGTAT GATGTTCAGG TTCCAATTGT GGACAAGATA
    AGGACCATTG CTCAGGCTGT CTATGGAGCC AAAGATATTG AACTCTCTCC TGAGGCACAA
    GCCAAAATAG ATCGTTACAC TCAACAGGGT TTTGGAAATT TGCCCATCTG CATGGCAAAG
    ACCCACCTTT CTCTATCTCA CCAACCTGAC AAAAAAGGTG TGCCAAGGGA CTTCATCTTA
    CCTATCAGTG ACGTCCGGGC CAGCATAGGC GCTGGGTTCA TTTACCCTTT GGTCGGAACG
    GCAAACCTGC TCATGTTCTT CGCTGAAATC AACCTCCCCT TTCTGGAATG GACATATGGA
    AAATTGGAGG CAAAATATAA TAATGGTGGT GACTCAGACT GCTTTAAGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66612
    Clone ID Related Accession (Same CDS sequence) XM_011535734.2 , XM_017010705.1
    Accession Version XM_011535734.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2631bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535734.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGGAAA TCAACCAGAA TTTGGCTGAG GAGGCTGGTC TGAACATCAC TCACATTTGC 
    CTCCCTCCAG ATAGCAGTGA AGCCGAGATT ATAGATGAAA TCTTAAAGAT CAATGAAGAT
    ACCAGAGTAC ATGGCCTTGC CCTTCAGATC TCTGAGAACT TGTTTAGCAA CAAAGTCCTC
    AATGCCTTGA AACCAGAAAA AGATGTGGAT GGAGTAACAG ACATAAACCT GGGGAAGCTG
    GTGCGAGGGG ATGCCCATGA ATGTTTTGTT TCACCTGTTG CCAAAGCTGT AATTGAACTT
    CTTGAAAAAT CAGGTGTCAA CCTAGATGGA AAGAAGATTT TGGTAGTGGG GGCCCATGGG
    TCTTTGGAAG CTGCTCTACA ATGCCTGTTC CAGAGAAAAG GGTCCATGAC AATGAGCATC
    CAGTGGAAAA CACGCCAGCT TCAAAGCAAG CTTCACGAGG CTGACATTGT GGTCCTAGGC
    TCACCTAAGC CAGAAGAGAT TCCCCTTACT TGGATACAAC CAGGAACTAC TGTTCTCAAC
    TGCTCCCATG ACTTCCTGTC AGGGAAGGTT GGGTGTGGCT CTCCAAGAAT ACATTTTGGT
    GGACTCATTG AGGAAGATGA TGTGATTCTC CTTGCTGCAG CTCTGCGAAT TCAGAACATG
    GTCAGTAGTG GAAGGAGATG GCTTCGTGAA CAGCAGCACA GGCGGTGGAG ACTTCACTGC
    TTGAAACTTC AGCCTCTCTC CCCTGTGCCA AGTGACATTG AGATTTCAAG AGGACAAACT
    CCAAAAGCTG TGGATGTCCT TGCCAAGGAG ATTGGATTGC TTGCAGATGA AATTGAAATC
    TATGGCAAAA GCAAAGCCAA AGTACGTTTG TCCGTGCTAG AAAGGTTAAA GGATCAAGCA
    GATGGAAAAT ACGTCTTAGT TGCTGGGATC ACACCCACCC CTCTTGGAGA AGGGAAGAGC
    ACAGTCACCA TCGGGCTTGT GCAGGCTCTG ACCGCACACC TGAATGTCAA CTCCTTTGCC
    TGCTTGAGGC AGCCTTCCCA AGGACCGACG TTTGGAGTGA AAGGAGGAGC CGCGGGTGGT
    GGATATGCCC AGGTCATCCC CATGGAGGAG TTCAACCTTC ACTTGACTGG AGACATCCAC
    GCCATCACCG CTGCCAATAA CTTGCTGGCT GCCGCCATCG ACACGAGGAT TCTTCATGAA
    AACACGCAAA CAGATAAGGC TCTGTATAAT CGGCTGGTTC CTTTAGTGAA TGGTGTCAGA
    GAATTTTCAG AAATTCAGCT TGCTCGGCTA AAAAAACTGG GAATAAATAA GACTGATCCG
    AGCACACTGA CAGAAGAGGA AGTGAGTAAA TTTGCCCGTC TCGACATCGA CCCATCTACC
    ATCACGTGGC AGAGAGTATT GGATACAAAT GACCGATTTC TACGAAAAAT AACCATCGGG
    CAGGGAAACA CAGAGAAGGG CCATTACCGG CAGGCGCAGT TTGACATCGC AGTGGCCAGC
    GAGATCATGG CGGTGCTGGC CCTGACGGAC AGCCTCGCAG ACATGAAGGC ACGGCTGGGA
    AGGATGGTGG TGGCCAGTGA CAAAAGCGGG CAGCCTGTGA CAGCAGATGA TTTGGGGGTG
    ACAGGTGCTT TGACAGTTTT GATGAAAGAT GCAATAAAAC CAAACCTGAT GCAGACCCTG
    GAAGGGACAC CTGTGTTCGT GCATGCGGGC CCTTTTGCTA ACATTGCTCA CGGCAACTCT
    TCAGTGTTGG CTGATAAAAT TGCCCTGAAA CTGGTTGGTG AAGAAGGATT TGTAGTGACC
    GAAGCTGGCT TTGGTGCTGA CATCGGAATG GAGAAATTCT TCAACATCAA GTGCCGAGCT
    TCCGGCTTGG TGCCCAACGT GGTTGTGTTA GTGGCAACGG TGCGAGCTCT GAAGATGCAT
    GGAGGCGGGC CAAGTGTAAC GGCTGGTGTT CCTCTTAAGA AAGAATATAC AGAGGAGAAC
    ATCCAGCTGG TGGCAGACGG CTGCTGTAAC CTCCAGAAGC AAATTCAGAT CACTCAGCTC
    TTTGGGGTTC CCGTTGTGGT GGCTCTGAAT GTCTTCAAGA CCGACACCCG CGCTGAGATT
    GACTTGGTGT GTGAGCTTGC AAAGCGGGCT GGTGCCTTTG ATGCAGTCCC CTGCTATCAC
    TGGTCCGTTG GTGGAAAAGG ATCGGTGGAC TTGGCTCGGG CTGTGAGAGA GGCTGCGAGT
    AAAAGAAGCC GATTCCAGTT CCTGTATGAT GTTCAGGTTC CAATTGTGGA CAAGATAAGG
    ACCATTGCTC AGGCTGTCTA TGGAGCCAAA GATATTGAAC TCTCTCCTGA GGCACAAGCC
    AAAATAGATC GTTACACTCA ACAGGGTTTT GGAAATTTGC CCATCTGCAT GGCAAAGACC
    CACCTTTCTC TATCTCACCA ACCTGACAAA AAAGGTGTGC CAAGGGACTT CATCTTACCT
    ATCAGTGACG TCCGGGCCAG CATAGGCGCT GGGTTCATTT ACCCTTTGGT CGGAACGGCA
    AACCTGCTCA TGTTCTTCGC TGAAATCAAC CTCCCCTTTC TGGAATGGAC ATATGGAAAA
    TTGGAGGCAA AATATAATAA TGGTGGTGAC TCAGACTGCT TTAAGGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011534036.1
    CDS255..2885
    Translation

    Target ORF information:

    RefSeq Version XM_011535734.2
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011535734.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGGAAA TCAACCAGAA TTTGGCTGAG GAGGCTGGTC TGAACATCAC TCACATTTGC 
    CTCCCTCCAG ATAGCAGTGA AGCCGAGATT ATAGATGAAA TCTTAAAGAT CAATGAAGAT
    ACCAGAGTAC ATGGCCTTGC CCTTCAGATC TCTGAGAACT TGTTTAGCAA CAAAGTCCTC
    AATGCCTTGA AACCAGAAAA AGATGTGGAT GGAGTAACAG ACATAAACCT GGGGAAGCTG
    GTGCGAGGGG ATGCCCATGA ATGTTTTGTT TCACCTGTTG CCAAAGCTGT AATTGAACTT
    CTTGAAAAAT CAGGTGTCAA CCTAGATGGA AAGAAGATTT TGGTAGTGGG GGCCCATGGG
    TCTTTGGAAG CTGCTCTACA ATGCCTGTTC CAGAGAAAAG GGTCCATGAC AATGAGCATC
    CAGTGGAAAA CACGCCAGCT TCAAAGCAAG CTTCACGAGG CTGACATTGT GGTCCTAGGC
    TCACCTAAGC CAGAAGAGAT TCCCCTTACT TGGATACAAC CAGGAACTAC TGTTCTCAAC
    TGCTCCCATG ACTTCCTGTC AGGGAAGGTT GGGTGTGGCT CTCCAAGAAT ACATTTTGGT
    GGACTCATTG AGGAAGATGA TGTGATTCTC CTTGCTGCAG CTCTGCGAAT TCAGAACATG
    GTCAGTAGTG GAAGGAGATG GCTTCGTGAA CAGCAGCACA GGCGGTGGAG ACTTCACTGC
    TTGAAACTTC AGCCTCTCTC CCCTGTGCCA AGTGACATTG AGATTTCAAG AGGACAAACT
    CCAAAAGCTG TGGATGTCCT TGCCAAGGAG ATTGGATTGC TTGCAGATGA AATTGAAATC
    TATGGCAAAA GCAAAGCCAA AGTACGTTTG TCCGTGCTAG AAAGGTTAAA GGATCAAGCA
    GATGGAAAAT ACGTCTTAGT TGCTGGGATC ACACCCACCC CTCTTGGAGA AGGGAAGAGC
    ACAGTCACCA TCGGGCTTGT GCAGGCTCTG ACCGCACACC TGAATGTCAA CTCCTTTGCC
    TGCTTGAGGC AGCCTTCCCA AGGACCGACG TTTGGAGTGA AAGGAGGAGC CGCGGGTGGT
    GGATATGCCC AGGTCATCCC CATGGAGGAG TTCAACCTTC ACTTGACTGG AGACATCCAC
    GCCATCACCG CTGCCAATAA CTTGCTGGCT GCCGCCATCG ACACGAGGAT TCTTCATGAA
    AACACGCAAA CAGATAAGGC TCTGTATAAT CGGCTGGTTC CTTTAGTGAA TGGTGTCAGA
    GAATTTTCAG AAATTCAGCT TGCTCGGCTA AAAAAACTGG GAATAAATAA GACTGATCCG
    AGCACACTGA CAGAAGAGGA AGTGAGTAAA TTTGCCCGTC TCGACATCGA CCCATCTACC
    ATCACGTGGC AGAGAGTATT GGATACAAAT GACCGATTTC TACGAAAAAT AACCATCGGG
    CAGGGAAACA CAGAGAAGGG CCATTACCGG CAGGCGCAGT TTGACATCGC AGTGGCCAGC
    GAGATCATGG CGGTGCTGGC CCTGACGGAC AGCCTCGCAG ACATGAAGGC ACGGCTGGGA
    AGGATGGTGG TGGCCAGTGA CAAAAGCGGG CAGCCTGTGA CAGCAGATGA TTTGGGGGTG
    ACAGGTGCTT TGACAGTTTT GATGAAAGAT GCAATAAAAC CAAACCTGAT GCAGACCCTG
    GAAGGGACAC CTGTGTTCGT GCATGCGGGC CCTTTTGCTA ACATTGCTCA CGGCAACTCT
    TCAGTGTTGG CTGATAAAAT TGCCCTGAAA CTGGTTGGTG AAGAAGGATT TGTAGTGACC
    GAAGCTGGCT TTGGTGCTGA CATCGGAATG GAGAAATTCT TCAACATCAA GTGCCGAGCT
    TCCGGCTTGG TGCCCAACGT GGTTGTGTTA GTGGCAACGG TGCGAGCTCT GAAGATGCAT
    GGAGGCGGGC CAAGTGTAAC GGCTGGTGTT CCTCTTAAGA AAGAATATAC AGAGGAGAAC
    ATCCAGCTGG TGGCAGACGG CTGCTGTAAC CTCCAGAAGC AAATTCAGAT CACTCAGCTC
    TTTGGGGTTC CCGTTGTGGT GGCTCTGAAT GTCTTCAAGA CCGACACCCG CGCTGAGATT
    GACTTGGTGT GTGAGCTTGC AAAGCGGGCT GGTGCCTTTG ATGCAGTCCC CTGCTATCAC
    TGGTCCGTTG GTGGAAAAGG ATCGGTGGAC TTGGCTCGGG CTGTGAGAGA GGCTGCGAGT
    AAAAGAAGCC GATTCCAGTT CCTGTATGAT GTTCAGGTTC CAATTGTGGA CAAGATAAGG
    ACCATTGCTC AGGCTGTCTA TGGAGCCAAA GATATTGAAC TCTCTCCTGA GGCACAAGCC
    AAAATAGATC GTTACACTCA ACAGGGTTTT GGAAATTTGC CCATCTGCAT GGCAAAGACC
    CACCTTTCTC TATCTCACCA ACCTGACAAA AAAGGTGTGC CAAGGGACTT CATCTTACCT
    ATCAGTGACG TCCGGGCCAG CATAGGCGCT GGGTTCATTT ACCCTTTGGT CGGAACGGCA
    AACCTGCTCA TGTTCTTCGC TGAAATCAAC CTCCCCTTTC TGGAATGGAC ATATGGAAAA
    TTGGAGGCAA AATATAATAA TGGTGGTGAC TCAGACTGCT TTAAGGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66612
    Clone ID Related Accession (Same CDS sequence) XM_011535734.2 , XM_017010705.1
    Accession Version XM_017010705.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2631bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGGAAA TCAACCAGAA TTTGGCTGAG GAGGCTGGTC TGAACATCAC TCACATTTGC 
    CTCCCTCCAG ATAGCAGTGA AGCCGAGATT ATAGATGAAA TCTTAAAGAT CAATGAAGAT
    ACCAGAGTAC ATGGCCTTGC CCTTCAGATC TCTGAGAACT TGTTTAGCAA CAAAGTCCTC
    AATGCCTTGA AACCAGAAAA AGATGTGGAT GGAGTAACAG ACATAAACCT GGGGAAGCTG
    GTGCGAGGGG ATGCCCATGA ATGTTTTGTT TCACCTGTTG CCAAAGCTGT AATTGAACTT
    CTTGAAAAAT CAGGTGTCAA CCTAGATGGA AAGAAGATTT TGGTAGTGGG GGCCCATGGG
    TCTTTGGAAG CTGCTCTACA ATGCCTGTTC CAGAGAAAAG GGTCCATGAC AATGAGCATC
    CAGTGGAAAA CACGCCAGCT TCAAAGCAAG CTTCACGAGG CTGACATTGT GGTCCTAGGC
    TCACCTAAGC CAGAAGAGAT TCCCCTTACT TGGATACAAC CAGGAACTAC TGTTCTCAAC
    TGCTCCCATG ACTTCCTGTC AGGGAAGGTT GGGTGTGGCT CTCCAAGAAT ACATTTTGGT
    GGACTCATTG AGGAAGATGA TGTGATTCTC CTTGCTGCAG CTCTGCGAAT TCAGAACATG
    GTCAGTAGTG GAAGGAGATG GCTTCGTGAA CAGCAGCACA GGCGGTGGAG ACTTCACTGC
    TTGAAACTTC AGCCTCTCTC CCCTGTGCCA AGTGACATTG AGATTTCAAG AGGACAAACT
    CCAAAAGCTG TGGATGTCCT TGCCAAGGAG ATTGGATTGC TTGCAGATGA AATTGAAATC
    TATGGCAAAA GCAAAGCCAA AGTACGTTTG TCCGTGCTAG AAAGGTTAAA GGATCAAGCA
    GATGGAAAAT ACGTCTTAGT TGCTGGGATC ACACCCACCC CTCTTGGAGA AGGGAAGAGC
    ACAGTCACCA TCGGGCTTGT GCAGGCTCTG ACCGCACACC TGAATGTCAA CTCCTTTGCC
    TGCTTGAGGC AGCCTTCCCA AGGACCGACG TTTGGAGTGA AAGGAGGAGC CGCGGGTGGT
    GGATATGCCC AGGTCATCCC CATGGAGGAG TTCAACCTTC ACTTGACTGG AGACATCCAC
    GCCATCACCG CTGCCAATAA CTTGCTGGCT GCCGCCATCG ACACGAGGAT TCTTCATGAA
    AACACGCAAA CAGATAAGGC TCTGTATAAT CGGCTGGTTC CTTTAGTGAA TGGTGTCAGA
    GAATTTTCAG AAATTCAGCT TGCTCGGCTA AAAAAACTGG GAATAAATAA GACTGATCCG
    AGCACACTGA CAGAAGAGGA AGTGAGTAAA TTTGCCCGTC TCGACATCGA CCCATCTACC
    ATCACGTGGC AGAGAGTATT GGATACAAAT GACCGATTTC TACGAAAAAT AACCATCGGG
    CAGGGAAACA CAGAGAAGGG CCATTACCGG CAGGCGCAGT TTGACATCGC AGTGGCCAGC
    GAGATCATGG CGGTGCTGGC CCTGACGGAC AGCCTCGCAG ACATGAAGGC ACGGCTGGGA
    AGGATGGTGG TGGCCAGTGA CAAAAGCGGG CAGCCTGTGA CAGCAGATGA TTTGGGGGTG
    ACAGGTGCTT TGACAGTTTT GATGAAAGAT GCAATAAAAC CAAACCTGAT GCAGACCCTG
    GAAGGGACAC CTGTGTTCGT GCATGCGGGC CCTTTTGCTA ACATTGCTCA CGGCAACTCT
    TCAGTGTTGG CTGATAAAAT TGCCCTGAAA CTGGTTGGTG AAGAAGGATT TGTAGTGACC
    GAAGCTGGCT TTGGTGCTGA CATCGGAATG GAGAAATTCT TCAACATCAA GTGCCGAGCT
    TCCGGCTTGG TGCCCAACGT GGTTGTGTTA GTGGCAACGG TGCGAGCTCT GAAGATGCAT
    GGAGGCGGGC CAAGTGTAAC GGCTGGTGTT CCTCTTAAGA AAGAATATAC AGAGGAGAAC
    ATCCAGCTGG TGGCAGACGG CTGCTGTAAC CTCCAGAAGC AAATTCAGAT CACTCAGCTC
    TTTGGGGTTC CCGTTGTGGT GGCTCTGAAT GTCTTCAAGA CCGACACCCG CGCTGAGATT
    GACTTGGTGT GTGAGCTTGC AAAGCGGGCT GGTGCCTTTG ATGCAGTCCC CTGCTATCAC
    TGGTCCGTTG GTGGAAAAGG ATCGGTGGAC TTGGCTCGGG CTGTGAGAGA GGCTGCGAGT
    AAAAGAAGCC GATTCCAGTT CCTGTATGAT GTTCAGGTTC CAATTGTGGA CAAGATAAGG
    ACCATTGCTC AGGCTGTCTA TGGAGCCAAA GATATTGAAC TCTCTCCTGA GGCACAAGCC
    AAAATAGATC GTTACACTCA ACAGGGTTTT GGAAATTTGC CCATCTGCAT GGCAAAGACC
    CACCTTTCTC TATCTCACCA ACCTGACAAA AAAGGTGTGC CAAGGGACTT CATCTTACCT
    ATCAGTGACG TCCGGGCCAG CATAGGCGCT GGGTTCATTT ACCCTTTGGT CGGAACGGCA
    AACCTGCTCA TGTTCTTCGC TGAAATCAAC CTCCCCTTTC TGGAATGGAC ATATGGAAAA
    TTGGAGGCAA AATATAATAA TGGTGGTGAC TCAGACTGCT TTAAGGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866194.1
    CDS659..3289
    Translation

    Target ORF information:

    RefSeq Version XM_017010705.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010705.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGGAAA TCAACCAGAA TTTGGCTGAG GAGGCTGGTC TGAACATCAC TCACATTTGC 
    CTCCCTCCAG ATAGCAGTGA AGCCGAGATT ATAGATGAAA TCTTAAAGAT CAATGAAGAT
    ACCAGAGTAC ATGGCCTTGC CCTTCAGATC TCTGAGAACT TGTTTAGCAA CAAAGTCCTC
    AATGCCTTGA AACCAGAAAA AGATGTGGAT GGAGTAACAG ACATAAACCT GGGGAAGCTG
    GTGCGAGGGG ATGCCCATGA ATGTTTTGTT TCACCTGTTG CCAAAGCTGT AATTGAACTT
    CTTGAAAAAT CAGGTGTCAA CCTAGATGGA AAGAAGATTT TGGTAGTGGG GGCCCATGGG
    TCTTTGGAAG CTGCTCTACA ATGCCTGTTC CAGAGAAAAG GGTCCATGAC AATGAGCATC
    CAGTGGAAAA CACGCCAGCT TCAAAGCAAG CTTCACGAGG CTGACATTGT GGTCCTAGGC
    TCACCTAAGC CAGAAGAGAT TCCCCTTACT TGGATACAAC CAGGAACTAC TGTTCTCAAC
    TGCTCCCATG ACTTCCTGTC AGGGAAGGTT GGGTGTGGCT CTCCAAGAAT ACATTTTGGT
    GGACTCATTG AGGAAGATGA TGTGATTCTC CTTGCTGCAG CTCTGCGAAT TCAGAACATG
    GTCAGTAGTG GAAGGAGATG GCTTCGTGAA CAGCAGCACA GGCGGTGGAG ACTTCACTGC
    TTGAAACTTC AGCCTCTCTC CCCTGTGCCA AGTGACATTG AGATTTCAAG AGGACAAACT
    CCAAAAGCTG TGGATGTCCT TGCCAAGGAG ATTGGATTGC TTGCAGATGA AATTGAAATC
    TATGGCAAAA GCAAAGCCAA AGTACGTTTG TCCGTGCTAG AAAGGTTAAA GGATCAAGCA
    GATGGAAAAT ACGTCTTAGT TGCTGGGATC ACACCCACCC CTCTTGGAGA AGGGAAGAGC
    ACAGTCACCA TCGGGCTTGT GCAGGCTCTG ACCGCACACC TGAATGTCAA CTCCTTTGCC
    TGCTTGAGGC AGCCTTCCCA AGGACCGACG TTTGGAGTGA AAGGAGGAGC CGCGGGTGGT
    GGATATGCCC AGGTCATCCC CATGGAGGAG TTCAACCTTC ACTTGACTGG AGACATCCAC
    GCCATCACCG CTGCCAATAA CTTGCTGGCT GCCGCCATCG ACACGAGGAT TCTTCATGAA
    AACACGCAAA CAGATAAGGC TCTGTATAAT CGGCTGGTTC CTTTAGTGAA TGGTGTCAGA
    GAATTTTCAG AAATTCAGCT TGCTCGGCTA AAAAAACTGG GAATAAATAA GACTGATCCG
    AGCACACTGA CAGAAGAGGA AGTGAGTAAA TTTGCCCGTC TCGACATCGA CCCATCTACC
    ATCACGTGGC AGAGAGTATT GGATACAAAT GACCGATTTC TACGAAAAAT AACCATCGGG
    CAGGGAAACA CAGAGAAGGG CCATTACCGG CAGGCGCAGT TTGACATCGC AGTGGCCAGC
    GAGATCATGG CGGTGCTGGC CCTGACGGAC AGCCTCGCAG ACATGAAGGC ACGGCTGGGA
    AGGATGGTGG TGGCCAGTGA CAAAAGCGGG CAGCCTGTGA CAGCAGATGA TTTGGGGGTG
    ACAGGTGCTT TGACAGTTTT GATGAAAGAT GCAATAAAAC CAAACCTGAT GCAGACCCTG
    GAAGGGACAC CTGTGTTCGT GCATGCGGGC CCTTTTGCTA ACATTGCTCA CGGCAACTCT
    TCAGTGTTGG CTGATAAAAT TGCCCTGAAA CTGGTTGGTG AAGAAGGATT TGTAGTGACC
    GAAGCTGGCT TTGGTGCTGA CATCGGAATG GAGAAATTCT TCAACATCAA GTGCCGAGCT
    TCCGGCTTGG TGCCCAACGT GGTTGTGTTA GTGGCAACGG TGCGAGCTCT GAAGATGCAT
    GGAGGCGGGC CAAGTGTAAC GGCTGGTGTT CCTCTTAAGA AAGAATATAC AGAGGAGAAC
    ATCCAGCTGG TGGCAGACGG CTGCTGTAAC CTCCAGAAGC AAATTCAGAT CACTCAGCTC
    TTTGGGGTTC CCGTTGTGGT GGCTCTGAAT GTCTTCAAGA CCGACACCCG CGCTGAGATT
    GACTTGGTGT GTGAGCTTGC AAAGCGGGCT GGTGCCTTTG ATGCAGTCCC CTGCTATCAC
    TGGTCCGTTG GTGGAAAAGG ATCGGTGGAC TTGGCTCGGG CTGTGAGAGA GGCTGCGAGT
    AAAAGAAGCC GATTCCAGTT CCTGTATGAT GTTCAGGTTC CAATTGTGGA CAAGATAAGG
    ACCATTGCTC AGGCTGTCTA TGGAGCCAAA GATATTGAAC TCTCTCCTGA GGCACAAGCC
    AAAATAGATC GTTACACTCA ACAGGGTTTT GGAAATTTGC CCATCTGCAT GGCAAAGACC
    CACCTTTCTC TATCTCACCA ACCTGACAAA AAAGGTGTGC CAAGGGACTT CATCTTACCT
    ATCAGTGACG TCCGGGCCAG CATAGGCGCT GGGTTCATTT ACCCTTTGGT CGGAACGGCA
    AACCTGCTCA TGTTCTTCGC TGAAATCAAC CTCCCCTTTC TGGAATGGAC ATATGGAAAA
    TTGGAGGCAA AATATAATAA TGGTGGTGAC TCAGACTGCT TTAAGGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101351
    Clone ID Related Accession (Same CDS sequence) NM_001350487.1
    Accession Version NM_001350487.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2607bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-15
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL035086.12, AL049694.9 and AL133260.12. On Apr 14, 2017 this sequence version replaced XM_017010707.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.253612.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGGAAA TCAACCAGAA TTTGGCTGAG GAGGCTGGTC TGAACATCAC TCACATTTGC 
    CTCCCTCCAG ATAGCAGTGA AGCCGAGATT ATAGATGAAA TCTTAAAGAT CAATGAAGAT
    ACCAGAGTAC ATGGCCTTGC CCTTCAGATC TCTGAGAACT TGTTTAGCAA CAAAGTCCTC
    AATGCCTTGA AACCAGAAAA AGATGTGGAT GGAGTAACAG ACATAAACCT GGGGAAGCTG
    GTGCGAGGGG ATGCCCATGA ATGTTTTGTT TCACCTGTTG CCAAAGCTGT AATTGAACTT
    CTTGAAAAAT CAGGTGTCAA CCTAGATGGA AAGAAGATTT TGGTAGTGGG GGCCCATGGG
    TCTTTGGAAG CTGCTCTACA ATGCCTGTTC CAGAGAAAAG GGTCCATGAC AATGAGCATC
    CAGTGGAAAA CACGCCAGCT TCAAAGCAAG CTTCACGAGG CTGACATTGT GGTCCTAGGC
    TCACCTAAGC CAGAAGAGAT TCCCCTTACT TGGATACAAC CAGGAACTAC TGTTCTCAAC
    TGCTCCCATG ACTTCCTGTC AGGGAAGGTT GGGTGTGGCT CTCCAAGAAT ACATTTTGGT
    GGACTCATTG AGGAAGATGA TGTGATTCTC CTTGCTGCAG CTCTGCGAAT TCAGAACATG
    GTCAGTAGTG GAAGGAGATG GCTTCGTGAA CAGCAGCACA GGCGGTGGAG ACTTCACTGC
    TTGAAACTTC AGCCTCTCTC CCCTGTGCCA AGTGACATTG AGATTTCAAG AGGACAAACT
    CCAAAAGCTG TGGATGTCCT TGCCAAGGAG ATTGGATTGC TTGCAGATGA AATTGAAATC
    TATGGCAAAA GCAAAGCCAA AGTACGTTTG TCCGTGCTAG AAAGGTTAAA GGATCAAGCA
    GATGGAAAAT ACGTCTTAGT TGCTGGGATC ACACCCACCC CTCTTGGAGA AGGGAAGAGC
    ACAGTCACCA TCGGGCTTGT GCAGGCTCTG ACCGCACACC TGAATGTCAA CTCCTTTGCC
    TGCTTGAGGC AGCCTTCCCA AGGACCGACG TTTGGAGTGA AAGGAGGAGC CGCGGGTGGT
    GGATATGCCC AGGTCATCCC CATGGAGGAG TTCAACCTTC ACTTGACTGG AGACATCCAC
    GCCATCACCG CTGCCAATAA CTTGCTGGCT GCCGCCATCG ACACGAGGAT TCTTCATGAA
    AACACGCAAA CAGATAAGGC TCTGTATAAT CGGCTGGTTC CTTTAGTGAA TGGTGTCAGA
    GAATTTTCAG AAATTCAGCT TGCTCGGCTA AAAAAACTGG GAATAAATAA GACTGATCCG
    AGCACACTGA CAGAAGAGGA AGTGAGTAAA TTTGCCCGTC TCGACATCGA CCCATCTACC
    ATCACGTGGC AGAGAGTATT GGATACAAAT GACCGATTTC TACGAAAAAT AACCATCGGG
    CAGGGAAACA CAGAGAAGGG CCATTACCGG CAGGCGCAGT TTGACATCGC AGTGGCCAGC
    GAGATCATGG CGGTGCTGGC CCTGACGGAC AGCCTCGCAG ACATGAAGGC ACGGCTGGGA
    AGGATGGTGG TGGCCAGTGA CAAAAGCGGG CAGCCTGTGA CAGCAGATGA TTTGGGGGTG
    ACAGGTGCTT TGACAGTTTT GATGAAAGAT GCAATAAAAC CAAACCTGAT GCAGACCCTG
    GAAGGGACAC CTGTGTTCGT GCATGCGGGC CCTTTTGCTA ACATTGCTCA CGGCAACTCT
    TCAGTGTTGG CTGATAAAAT TGCCCTGAAA CTGGTTGGTG AAGAAGGATT TGTAGTGACC
    GAAGCTGGCT TTGGTGCTGA CATCGGAATG GAGAAATTCT TCAACATCAA GTGCCGAGCT
    TCCGGCTTGG TGCCCAACGT GGTTGTGTTA GTGGCAACGG TGCGAGCTCT GAAGATGCAT
    GGAGGCGGGC CAAGTGTAAC GGCTGGTGTT CCTCTTAAGA AAGAATATAC AGAGGAGAAC
    ATCCAGCTGG TGGCAGACGG CTGCTGTAAC CTCCAGAAGC AAATTCAGAT CACTCAGCTC
    TTTGGGGTTC CCGTTGTGGT GGCTCTGAAT GTCTTCAAGA CCGACACCCG CGCTGAGATT
    GACTTGGTGT GTGAGCTTGC AAAGCGGGCT GGTGCCTTTG ATGCAGTCCC CTGCTATCAC
    TGGTCCGTTG GTGGAAAAGG ATCGGTGGAC TTGGCTCGGG CTGTGAGAGA GGCTGCGAGT
    AAAAGAAGCC GATTCCAGTT CCTGTATGAT GTTCAGGTTC CAATTGTGGA CAAGATAAGG
    ACCATTGCTC AGGCTGTCTA TGGAGCCAAA GATATTGAAC TCTCTCCTGA GGCACAAGCC
    AAAATAGATC GTTACACTCA ACAGGGTTTT GGAAATTTGC CCATCTGCAT GGCAAAGACC
    CACCTTTCTC TATCTCACCA ACCTGACAAA AAAGGTGTGC CAAGGGACTT CATCTTACCT
    ATCAGTGACG TCCGGGCCAG CATAGGCGCT GGGTTCATTT ACCCTTTGGT CGGAACGATG
    AGCACCATGC CAGGACTGCC CACCCGGCCC TGCTTTTATG ACATAGATCT TGATACCGAA
    ACAGAACAAG TTAAAGGCTT GTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337416.1
    CDS1240..3846
    Translation

    Target ORF information:

    RefSeq Version NM_001350487.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350487.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGGAAA TCAACCAGAA TTTGGCTGAG GAGGCTGGTC TGAACATCAC TCACATTTGC 
    CTCCCTCCAG ATAGCAGTGA AGCCGAGATT ATAGATGAAA TCTTAAAGAT CAATGAAGAT
    ACCAGAGTAC ATGGCCTTGC CCTTCAGATC TCTGAGAACT TGTTTAGCAA CAAAGTCCTC
    AATGCCTTGA AACCAGAAAA AGATGTGGAT GGAGTAACAG ACATAAACCT GGGGAAGCTG
    GTGCGAGGGG ATGCCCATGA ATGTTTTGTT TCACCTGTTG CCAAAGCTGT AATTGAACTT
    CTTGAAAAAT CAGGTGTCAA CCTAGATGGA AAGAAGATTT TGGTAGTGGG GGCCCATGGG
    TCTTTGGAAG CTGCTCTACA ATGCCTGTTC CAGAGAAAAG GGTCCATGAC AATGAGCATC
    CAGTGGAAAA CACGCCAGCT TCAAAGCAAG CTTCACGAGG CTGACATTGT GGTCCTAGGC
    TCACCTAAGC CAGAAGAGAT TCCCCTTACT TGGATACAAC CAGGAACTAC TGTTCTCAAC
    TGCTCCCATG ACTTCCTGTC AGGGAAGGTT GGGTGTGGCT CTCCAAGAAT ACATTTTGGT
    GGACTCATTG AGGAAGATGA TGTGATTCTC CTTGCTGCAG CTCTGCGAAT TCAGAACATG
    GTCAGTAGTG GAAGGAGATG GCTTCGTGAA CAGCAGCACA GGCGGTGGAG ACTTCACTGC
    TTGAAACTTC AGCCTCTCTC CCCTGTGCCA AGTGACATTG AGATTTCAAG AGGACAAACT
    CCAAAAGCTG TGGATGTCCT TGCCAAGGAG ATTGGATTGC TTGCAGATGA AATTGAAATC
    TATGGCAAAA GCAAAGCCAA AGTACGTTTG TCCGTGCTAG AAAGGTTAAA GGATCAAGCA
    GATGGAAAAT ACGTCTTAGT TGCTGGGATC ACACCCACCC CTCTTGGAGA AGGGAAGAGC
    ACAGTCACCA TCGGGCTTGT GCAGGCTCTG ACCGCACACC TGAATGTCAA CTCCTTTGCC
    TGCTTGAGGC AGCCTTCCCA AGGACCGACG TTTGGAGTGA AAGGAGGAGC CGCGGGTGGT
    GGATATGCCC AGGTCATCCC CATGGAGGAG TTCAACCTTC ACTTGACTGG AGACATCCAC
    GCCATCACCG CTGCCAATAA CTTGCTGGCT GCCGCCATCG ACACGAGGAT TCTTCATGAA
    AACACGCAAA CAGATAAGGC TCTGTATAAT CGGCTGGTTC CTTTAGTGAA TGGTGTCAGA
    GAATTTTCAG AAATTCAGCT TGCTCGGCTA AAAAAACTGG GAATAAATAA GACTGATCCG
    AGCACACTGA CAGAAGAGGA AGTGAGTAAA TTTGCCCGTC TCGACATCGA CCCATCTACC
    ATCACGTGGC AGAGAGTATT GGATACAAAT GACCGATTTC TACGAAAAAT AACCATCGGG
    CAGGGAAACA CAGAGAAGGG CCATTACCGG CAGGCGCAGT TTGACATCGC AGTGGCCAGC
    GAGATCATGG CGGTGCTGGC CCTGACGGAC AGCCTCGCAG ACATGAAGGC ACGGCTGGGA
    AGGATGGTGG TGGCCAGTGA CAAAAGCGGG CAGCCTGTGA CAGCAGATGA TTTGGGGGTG
    ACAGGTGCTT TGACAGTTTT GATGAAAGAT GCAATAAAAC CAAACCTGAT GCAGACCCTG
    GAAGGGACAC CTGTGTTCGT GCATGCGGGC CCTTTTGCTA ACATTGCTCA CGGCAACTCT
    TCAGTGTTGG CTGATAAAAT TGCCCTGAAA CTGGTTGGTG AAGAAGGATT TGTAGTGACC
    GAAGCTGGCT TTGGTGCTGA CATCGGAATG GAGAAATTCT TCAACATCAA GTGCCGAGCT
    TCCGGCTTGG TGCCCAACGT GGTTGTGTTA GTGGCAACGG TGCGAGCTCT GAAGATGCAT
    GGAGGCGGGC CAAGTGTAAC GGCTGGTGTT CCTCTTAAGA AAGAATATAC AGAGGAGAAC
    ATCCAGCTGG TGGCAGACGG CTGCTGTAAC CTCCAGAAGC AAATTCAGAT CACTCAGCTC
    TTTGGGGTTC CCGTTGTGGT GGCTCTGAAT GTCTTCAAGA CCGACACCCG CGCTGAGATT
    GACTTGGTGT GTGAGCTTGC AAAGCGGGCT GGTGCCTTTG ATGCAGTCCC CTGCTATCAC
    TGGTCCGTTG GTGGAAAAGG ATCGGTGGAC TTGGCTCGGG CTGTGAGAGA GGCTGCGAGT
    AAAAGAAGCC GATTCCAGTT CCTGTATGAT GTTCAGGTTC CAATTGTGGA CAAGATAAGG
    ACCATTGCTC AGGCTGTCTA TGGAGCCAAA GATATTGAAC TCTCTCCTGA GGCACAAGCC
    AAAATAGATC GTTACACTCA ACAGGGTTTT GGAAATTTGC CCATCTGCAT GGCAAAGACC
    CACCTTTCTC TATCTCACCA ACCTGACAAA AAAGGTGTGC CAAGGGACTT CATCTTACCT
    ATCAGTGACG TCCGGGCCAG CATAGGCGCT GGGTTCATTT ACCCTTTGGT CGGAACGATG
    AGCACCATGC CAGGACTGCC CACCCGGCCC TGCTTTTATG ACATAGATCT TGATACCGAA
    ACAGAACAAG TTAAAGGCTT GTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101372
    Clone ID Related Accession (Same CDS sequence) NM_001350486.1
    Accession Version NM_001350486.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-16
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL035086.12, AL049694.9 and AL133260.12. On Apr 14, 2017 this sequence version replaced XM_017010704.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.170842.1, SRR1803613.257987.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAATCGC GCCGGGCGCG ACCCAGACGA GAAGTCATTC AGAATTCAAA AGAAGTTCTA 
    AGTTTATTGC AAGAAAAAAA CCCTGCCTTC AAGCCGGTTC TTGCAATTAT CCAGGCAGGT
    GACGACAACT TGATGCAGGA AATCAACCAG AATTTGGCTG AGGAGGCTGG TCTGAACATC
    ACTCACATTT GCCTCCCTCC AGATAGCAGT GAAGCCGAGA TTATAGATGA AATCTTAAAG
    ATCAATGAAG ATACCAGAGT ACATGGCCTT GCCCTTCAGA TCTCTGAGAA CTTGTTTAGC
    AACAAAGTCC TCAATGCCTT GAAACCAGAA AAAGATGTGG ATGGAGTAAC AGACATAAAC
    CTGGGGAAGC TGGTGCGAGG GGATGCCCAT GAATGTTTTG TTTCACCTGT TGCCAAAGCT
    GTAATTGAAC TTCTTGAAAA ATCAGGTGTC AACCTAGATG GAAAGAAGAT TTTGGTAGTG
    GGGGCCCATG GGTCTTTGGA AGCTGCTCTA CAATGCCTGT TCCAGAGAAA AGGGTCCATG
    ACAATGAGCA TCCAGTGGAA AACACGCCAG CTTCAAAGCA AGCTTCACGA GGCTGACATT
    GTGGTCCTAG GCTCACCTAA GCCAGAAGAG ATTCCCCTTA CTTGGATACA ACCAGGAACT
    ACTGTTCTCA ACTGCTCCCA TGACTTCCTG TCAGGGAAGG TTGGGTGTGG CTCTCCAAGA
    ATACATTTTG GTGGACTCAT TGAGGAAGAT GATGTGATTC TCCTTGCTGC AGCTCTGCGA
    ATTCAGAACA TGGTCAGTAG TGGAAGGAGA TGGCTTCGTG AACAGCAGCA CAGGCGGTGG
    AGACTTCACT GCTTGAAACT TCAGCCTCTC TCCCCTGTGC CAAGTGACAT TGAGATTTCA
    AGAGGACAAA CTCCAAAAGC TGTGGATGTC CTTGCCAAGG AGATTGGATT GCTTGCAGAT
    GAAATTGAAA TCTATGGCAA AAGCAAAGCC AAAGTACGTT TGTCCGTGCT AGAAAGGTTA
    AAGGATCAAG CAGATGGAAA ATACGTCTTA GTTGCTGGGA TCACACCCAC CCCTCTTGGA
    GAAGGGAAGA GCACAGTCAC CATCGGGCTT GTGCAGGCTC TGACCGCACA CCTGAATGTC
    AACTCCTTTG CCTGCTTGAG GCAGCCTTCC CAAGGACCGA CGTTTGGAGT GAAAGGAGGA
    GCCGCGGGTG GTGGATATGC CCAGGTCATC CCCATGGAGG AGTTCAACCT TCACTTGACT
    GGAGACATCC ACGCCATCAC CGCTGCCAAT AACTTGCTGG CTGCCGCCAT CGACACGAGG
    ATTCTTCATG AAAACACGCA AACAGATAAG GCTCTGTATA ATCGGCTGGT TCCTTTAGTG
    AATGGTGTCA GAGAATTTTC AGAAATTCAG CTTGCTCGGC TAAAAAAACT GGGAATAAAT
    AAGACTGATC CGAGCACACT GACAGAAGAG GAAGTGAGTA AATTTGCCCG TCTCGACATC
    GACCCATCTA CCATCACGTG GCAGAGAGTA TTGGATACAA ATGACCGATT TCTACGAAAA
    ATAACCATCG GGCAGGGAAA CACAGAGAAG GGCCATTACC GGCAGGCGCA GTTTGACATC
    GCAGTGGCCA GCGAGATCAT GGCGGTGCTG GCCCTGACGG ACAGCCTCGC AGACATGAAG
    GCACGGCTGG GAAGGATGGT GGTGGCCAGT GACAAAAGCG GGCAGCCTGT GACAGCAGAT
    GATTTGGGGG TGACAGGTGC TTTGACAGTT TTGATGAAAG ATGCAATAAA ACCAAACCTG
    ATGCAGACCC TGGAAGGGAC ACCTGTGTTC GTGCATGCGG GCCCTTTTGC TAACATTGCT
    CACGGCAACT CTTCAGTGTT GGCTGATAAA ATTGCCCTGA AACTGGTTGG TGAAGAAGGA
    TTTGTAGTGA CCGAAGCTGG CTTTGGTGCT GACATCGGAA TGGAGAAATT CTTCAACATC
    AAGTGCCGAG CTTCCGGCTT GGTGCCCAAC GTGGTTGTGT TAGTGGCAAC GGTGCGAGCT
    CTGAAGATGC ATGGAGGCGG GCCAAGTGTA ACGGCTGGTG TTCCTCTTAA GAAAGAATAT
    ACAGAGGAGA ACATCCAGCT GGTGGCAGAC GGCTGCTGTA ACCTCCAGAA GCAAATTCAG
    ATCACTCAGC TCTTTGGGGT TCCCGTTGTG GTGGCTCTGA ATGTCTTCAA GACCGACACC
    CGCGCTGAGA TTGACTTGGT GTGTGAGCTT GCAAAGCGGG CTGGTGCCTT TGATGCAGTC
    CCCTGCTATC ACTGGTCCGT TGGTGGAAAA GGATCGGTGG ACTTGGCTCG GGCTGTGAGA
    GAGGCTGCGA GTAAAAGAAG CCGATTCCAG TTCCTGTATG ATGTTCAGGT TCCAATTGTG
    GACAAGATAA GGACCATTGC TCAGGCTGTC TATGGAGCCA AAGATATTGA ACTCTCTCCT
    GAGGCACAAG CCAAAATAGA TCGTTACACT CAACAGGGTT TTGGAAATTT GCCCATCTGC
    ATGGCAAAGA CCCACCTTTC TCTATCTCAC CAACCTGACA AAAAAGGTGT GCCAAGGGAC
    TTCATCTTAC CTATCAGTGA CGTCCGGGCC AGCATAGGCG CTGGGTTCAT TTACCCTTTG
    GTCGGAACGA TGAGCACCAT GCCAGGACTG CCCACCCGGC CCTGCTTTTA TGACATAGAT
    CTTGATACCG AAACAGAACA AGTTAAAGGC TTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337415.1
    CDS27..2765
    Translation

    Target ORF information:

    RefSeq Version NM_001350486.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350486.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAATCGC GCCGGGCGCG ACCCAGACGA GAAGTCATTC AGAATTCAAA AGAAGTTCTA 
    AGTTTATTGC AAGAAAAAAA CCCTGCCTTC AAGCCGGTTC TTGCAATTAT CCAGGCAGGT
    GACGACAACT TGATGCAGGA AATCAACCAG AATTTGGCTG AGGAGGCTGG TCTGAACATC
    ACTCACATTT GCCTCCCTCC AGATAGCAGT GAAGCCGAGA TTATAGATGA AATCTTAAAG
    ATCAATGAAG ATACCAGAGT ACATGGCCTT GCCCTTCAGA TCTCTGAGAA CTTGTTTAGC
    AACAAAGTCC TCAATGCCTT GAAACCAGAA AAAGATGTGG ATGGAGTAAC AGACATAAAC
    CTGGGGAAGC TGGTGCGAGG GGATGCCCAT GAATGTTTTG TTTCACCTGT TGCCAAAGCT
    GTAATTGAAC TTCTTGAAAA ATCAGGTGTC AACCTAGATG GAAAGAAGAT TTTGGTAGTG
    GGGGCCCATG GGTCTTTGGA AGCTGCTCTA CAATGCCTGT TCCAGAGAAA AGGGTCCATG
    ACAATGAGCA TCCAGTGGAA AACACGCCAG CTTCAAAGCA AGCTTCACGA GGCTGACATT
    GTGGTCCTAG GCTCACCTAA GCCAGAAGAG ATTCCCCTTA CTTGGATACA ACCAGGAACT
    ACTGTTCTCA ACTGCTCCCA TGACTTCCTG TCAGGGAAGG TTGGGTGTGG CTCTCCAAGA
    ATACATTTTG GTGGACTCAT TGAGGAAGAT GATGTGATTC TCCTTGCTGC AGCTCTGCGA
    ATTCAGAACA TGGTCAGTAG TGGAAGGAGA TGGCTTCGTG AACAGCAGCA CAGGCGGTGG
    AGACTTCACT GCTTGAAACT TCAGCCTCTC TCCCCTGTGC CAAGTGACAT TGAGATTTCA
    AGAGGACAAA CTCCAAAAGC TGTGGATGTC CTTGCCAAGG AGATTGGATT GCTTGCAGAT
    GAAATTGAAA TCTATGGCAA AAGCAAAGCC AAAGTACGTT TGTCCGTGCT AGAAAGGTTA
    AAGGATCAAG CAGATGGAAA ATACGTCTTA GTTGCTGGGA TCACACCCAC CCCTCTTGGA
    GAAGGGAAGA GCACAGTCAC CATCGGGCTT GTGCAGGCTC TGACCGCACA CCTGAATGTC
    AACTCCTTTG CCTGCTTGAG GCAGCCTTCC CAAGGACCGA CGTTTGGAGT GAAAGGAGGA
    GCCGCGGGTG GTGGATATGC CCAGGTCATC CCCATGGAGG AGTTCAACCT TCACTTGACT
    GGAGACATCC ACGCCATCAC CGCTGCCAAT AACTTGCTGG CTGCCGCCAT CGACACGAGG
    ATTCTTCATG AAAACACGCA AACAGATAAG GCTCTGTATA ATCGGCTGGT TCCTTTAGTG
    AATGGTGTCA GAGAATTTTC AGAAATTCAG CTTGCTCGGC TAAAAAAACT GGGAATAAAT
    AAGACTGATC CGAGCACACT GACAGAAGAG GAAGTGAGTA AATTTGCCCG TCTCGACATC
    GACCCATCTA CCATCACGTG GCAGAGAGTA TTGGATACAA ATGACCGATT TCTACGAAAA
    ATAACCATCG GGCAGGGAAA CACAGAGAAG GGCCATTACC GGCAGGCGCA GTTTGACATC
    GCAGTGGCCA GCGAGATCAT GGCGGTGCTG GCCCTGACGG ACAGCCTCGC AGACATGAAG
    GCACGGCTGG GAAGGATGGT GGTGGCCAGT GACAAAAGCG GGCAGCCTGT GACAGCAGAT
    GATTTGGGGG TGACAGGTGC TTTGACAGTT TTGATGAAAG ATGCAATAAA ACCAAACCTG
    ATGCAGACCC TGGAAGGGAC ACCTGTGTTC GTGCATGCGG GCCCTTTTGC TAACATTGCT
    CACGGCAACT CTTCAGTGTT GGCTGATAAA ATTGCCCTGA AACTGGTTGG TGAAGAAGGA
    TTTGTAGTGA CCGAAGCTGG CTTTGGTGCT GACATCGGAA TGGAGAAATT CTTCAACATC
    AAGTGCCGAG CTTCCGGCTT GGTGCCCAAC GTGGTTGTGT TAGTGGCAAC GGTGCGAGCT
    CTGAAGATGC ATGGAGGCGG GCCAAGTGTA ACGGCTGGTG TTCCTCTTAA GAAAGAATAT
    ACAGAGGAGA ACATCCAGCT GGTGGCAGAC GGCTGCTGTA ACCTCCAGAA GCAAATTCAG
    ATCACTCAGC TCTTTGGGGT TCCCGTTGTG GTGGCTCTGA ATGTCTTCAA GACCGACACC
    CGCGCTGAGA TTGACTTGGT GTGTGAGCTT GCAAAGCGGG CTGGTGCCTT TGATGCAGTC
    CCCTGCTATC ACTGGTCCGT TGGTGGAAAA GGATCGGTGG ACTTGGCTCG GGCTGTGAGA
    GAGGCTGCGA GTAAAAGAAG CCGATTCCAG TTCCTGTATG ATGTTCAGGT TCCAATTGTG
    GACAAGATAA GGACCATTGC TCAGGCTGTC TATGGAGCCA AAGATATTGA ACTCTCTCCT
    GAGGCACAAG CCAAAATAGA TCGTTACACT CAACAGGGTT TTGGAAATTT GCCCATCTGC
    ATGGCAAAGA CCCACCTTTC TCTATCTCAC CAACCTGACA AAAAAGGTGT GCCAAGGGAC
    TTCATCTTAC CTATCAGTGA CGTCCGGGCC AGCATAGGCG CTGGGTTCAT TTACCCTTTG
    GTCGGAACGA TGAGCACCAT GCCAGGACTG CCCACCCGGC CCTGCTTTTA TGACATAGAT
    CTTGATACCG AAACAGAACA AGTTAAAGGC TTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu105789
    Clone ID Related Accession (Same CDS sequence) XM_024446395.1
    Accession Version XM_024446395.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2832bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAGTCTC GCTTTGTTGC TCAGGCTGGA GTTCAGTGGT GCCATCTCGG CTCAATGCAG 
    CCTCCGCCTC CTGGGCTCAA GCGATTCTCC CGCCTTAGCC TCCAGTGTAA CTGGGACCAC
    AGAGAAGTCA TTCAGAATTC AAAAGAAGTT CTAAGTTTAT TGCAAGAAAA AAACCCTGCC
    TTCAAGCCGG TTCTTGCAAT TATCCAGGCA GGTGACGACA ACTTGATGCA GGAAATCAAC
    CAGAATTTGG CTGAGGAGGC TGGTCTGAAC ATCACTCACA TTTGCCTCCC TCCAGATAGC
    AGTGAAGCCG AGATTATAGA TGAAATCTTA AAGATCAATG AAGATACCAG AGTACATGGC
    CTTGCCCTTC AGATCTCTGA GAACTTGTTT AGCAACAAAG TCCTCAATGC CTTGAAACCA
    GAAAAAGATG TGGATGGAGT AACAGACATA AACCTGGGGA AGCTGGTGCG AGGGGATGCC
    CATGAATGTT TTGTTTCACC TGTTGCCAAA GCTGTAATTG AACTTCTTGA AAAATCAGGT
    GTCAACCTAG ATGGAAAGAA GATTTTGGTA GTGGGGGCCC ATGGGTCTTT GGAAGCTGCT
    CTACAATGCC TGTTCCAGAG AAAAGGGTCC ATGACAATGA GCATCCAGTG GAAAACACGC
    CAGCTTCAAA GCAAGCTTCA CGAGGCTGAC ATTGTGGTCC TAGGCTCACC TAAGCCAGAA
    GAGATTCCCC TTACTTGGAT ACAACCAGGA ACTACTGTTC TCAACTGCTC CCATGACTTC
    CTGTCAGGGA AGGTTGGGTG TGGCTCTCCA AGAATACATT TTGGTGGACT CATTGAGGAA
    GATGATGTGA TTCTCCTTGC TGCAGCTCTG CGAATTCAGA ACATGGTCAG TAGTGGAAGG
    AGATGGCTTC GTGAACAGCA GCACAGGCGG TGGAGACTTC ACTGCTTGAA ACTTCAGCCT
    CTCTCCCCTG TGCCAAGTGA CATTGAGATT TCAAGAGGAC AAACTCCAAA AGCTGTGGAT
    GTCCTTGCCA AGGAGATTGG ATTGCTTGCA GATGAAATTG AAATCTATGG CAAAAGCAAA
    GCCAAAGTAC GTTTGTCCGT GCTAGAAAGG TTAAAGGATC AAGCAGATGG AAAATACGTC
    TTAGTTGCTG GGATCACACC CACCCCTCTT GGAGAAGGGA AGAGCACAGT CACCATCGGG
    CTTGTGCAGG CTCTGACCGC ACACCTGAAT GTCAACTCCT TTGCCTGCTT GAGGCAGCCT
    TCCCAAGGAC CGACGTTTGG AGTGAAAGGA GGAGCCGCGG GTGGTGGATA TGCCCAGGTC
    ATCCCCATGG AGGAGTTCAA CCTTCACTTG ACTGGAGACA TCCACGCCAT CACCGCTGCC
    AATAACTTGC TGGCTGCCGC CATCGACACG AGGATTCTTC ATGAAAACAC GCAAACAGAT
    AAGGCTCTGT ATAATCGGCT GGTTCCTTTA GTGAATGGTG TCAGAGAATT TTCAGAAATT
    CAGCTTGCTC GGCTAAAAAA ACTGGGAATA AATAAGACTG ATCCGAGCAC ACTGACAGAA
    GAGGAAGTGA GTAAATTTGC CCGTCTCGAC ATCGACCCAT CTACCATCAC GTGGCAGAGA
    GTATTGGATA CAAATGACCG ATTTCTACGA AAAATAACCA TCGGGCAGGG AAACACAGAG
    AAGGGCCATT ACCGGCAGGC GCAGTTTGAC ATCGCAGTGG CCAGCGAGAT CATGGCGGTG
    CTGGCCCTGA CGGACAGCCT CGCAGACATG AAGGCACGGC TGGGAAGGAT GGTGGTGGCC
    AGTGACAAAA GCGGGCAGCC TGTGACAGCA GATGATTTGG GGGTGACAGG TGCTTTGACA
    GTTTTGATGA AAGATGCAAT AAAACCAAAC CTGATGCAGA CCCTGGAAGG GACACCTGTG
    TTCGTGCATG CGGGCCCTTT TGCTAACATT GCTCACGGCA ACTCTTCAGT GTTGGCTGAT
    AAAATTGCCC TGAAACTGGT TGGTGAAGAA GGATTTGTAG TGACCGAAGC TGGCTTTGGT
    GCTGACATCG GAATGGAGAA ATTCTTCAAC ATCAAGTGCC GAGCTTCCGG CTTGGTGCCC
    AACGTGGTTG TGTTAGTGGC AACGGTGCGA GCTCTGAAGA TGCATGGAGG CGGGCCAAGT
    GTAACGGCTG GTGTTCCTCT TAAGAAAGAA TATACAGAGG AGAACATCCA GCTGGTGGCA
    GACGGCTGCT GTAACCTCCA GAAGCAAATT CAGATCACTC AGCTCTTTGG GGTTCCCGTT
    GTGGTGGCTC TGAATGTCTT CAAGACCGAC ACCCGCGCTG AGATTGACTT GGTGTGTGAG
    CTTGCAAAGC GGGCTGGTGC CTTTGATGCA GTCCCCTGCT ATCACTGGTC CGTTGGTGGA
    AAAGGATCGG TGGACTTGGC TCGGGCTGTG AGAGAGGCTG CGAGTAAAAG AAGCCGATTC
    CAGTTCCTGT ATGATGTTCA GGTTCCAATT GTGGACAAGA TAAGGACCAT TGCTCAGGCT
    GTCTATGGAG CCAAAGATAT TGAACTCTCT CCTGAGGCAC AAGCCAAAAT AGATCGTTAC
    ACTCAACAGG GTTTTGGAAA TTTGCCCATC TGCATGGCAA AGACCCACCT TTCTCTATCT
    CACCAACCTG ACAAAAAAGG TGTGCCAAGG GACTTCATCT TACCTATCAG TGACGTCCGG
    GCCAGCATAG GCGCTGGGTT CATTTACCCT TTGGTCGGAA CGATGAGCAC CATGCCAGGA
    CTGCCCACCC GGCCCTGCTT TTATGACATA GATCTTGATA CCGAAACAGA ACAAGTTAAA
    GGCTTGTTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024302163.1
    CDS570..3401
    Translation

    Target ORF information:

    RefSeq Version XM_024446395.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024446395.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAGTCTC GCTTTGTTGC TCAGGCTGGA GTTCAGTGGT GCCATCTCGG CTCAATGCAG 
    CCTCCGCCTC CTGGGCTCAA GCGATTCTCC CGCCTTAGCC TCCAGTGTAA CTGGGACCAC
    AGAGAAGTCA TTCAGAATTC AAAAGAAGTT CTAAGTTTAT TGCAAGAAAA AAACCCTGCC
    TTCAAGCCGG TTCTTGCAAT TATCCAGGCA GGTGACGACA ACTTGATGCA GGAAATCAAC
    CAGAATTTGG CTGAGGAGGC TGGTCTGAAC ATCACTCACA TTTGCCTCCC TCCAGATAGC
    AGTGAAGCCG AGATTATAGA TGAAATCTTA AAGATCAATG AAGATACCAG AGTACATGGC
    CTTGCCCTTC AGATCTCTGA GAACTTGTTT AGCAACAAAG TCCTCAATGC CTTGAAACCA
    GAAAAAGATG TGGATGGAGT AACAGACATA AACCTGGGGA AGCTGGTGCG AGGGGATGCC
    CATGAATGTT TTGTTTCACC TGTTGCCAAA GCTGTAATTG AACTTCTTGA AAAATCAGGT
    GTCAACCTAG ATGGAAAGAA GATTTTGGTA GTGGGGGCCC ATGGGTCTTT GGAAGCTGCT
    CTACAATGCC TGTTCCAGAG AAAAGGGTCC ATGACAATGA GCATCCAGTG GAAAACACGC
    CAGCTTCAAA GCAAGCTTCA CGAGGCTGAC ATTGTGGTCC TAGGCTCACC TAAGCCAGAA
    GAGATTCCCC TTACTTGGAT ACAACCAGGA ACTACTGTTC TCAACTGCTC CCATGACTTC
    CTGTCAGGGA AGGTTGGGTG TGGCTCTCCA AGAATACATT TTGGTGGACT CATTGAGGAA
    GATGATGTGA TTCTCCTTGC TGCAGCTCTG CGAATTCAGA ACATGGTCAG TAGTGGAAGG
    AGATGGCTTC GTGAACAGCA GCACAGGCGG TGGAGACTTC ACTGCTTGAA ACTTCAGCCT
    CTCTCCCCTG TGCCAAGTGA CATTGAGATT TCAAGAGGAC AAACTCCAAA AGCTGTGGAT
    GTCCTTGCCA AGGAGATTGG ATTGCTTGCA GATGAAATTG AAATCTATGG CAAAAGCAAA
    GCCAAAGTAC GTTTGTCCGT GCTAGAAAGG TTAAAGGATC AAGCAGATGG AAAATACGTC
    TTAGTTGCTG GGATCACACC CACCCCTCTT GGAGAAGGGA AGAGCACAGT CACCATCGGG
    CTTGTGCAGG CTCTGACCGC ACACCTGAAT GTCAACTCCT TTGCCTGCTT GAGGCAGCCT
    TCCCAAGGAC CGACGTTTGG AGTGAAAGGA GGAGCCGCGG GTGGTGGATA TGCCCAGGTC
    ATCCCCATGG AGGAGTTCAA CCTTCACTTG ACTGGAGACA TCCACGCCAT CACCGCTGCC
    AATAACTTGC TGGCTGCCGC CATCGACACG AGGATTCTTC ATGAAAACAC GCAAACAGAT
    AAGGCTCTGT ATAATCGGCT GGTTCCTTTA GTGAATGGTG TCAGAGAATT TTCAGAAATT
    CAGCTTGCTC GGCTAAAAAA ACTGGGAATA AATAAGACTG ATCCGAGCAC ACTGACAGAA
    GAGGAAGTGA GTAAATTTGC CCGTCTCGAC ATCGACCCAT CTACCATCAC GTGGCAGAGA
    GTATTGGATA CAAATGACCG ATTTCTACGA AAAATAACCA TCGGGCAGGG AAACACAGAG
    AAGGGCCATT ACCGGCAGGC GCAGTTTGAC ATCGCAGTGG CCAGCGAGAT CATGGCGGTG
    CTGGCCCTGA CGGACAGCCT CGCAGACATG AAGGCACGGC TGGGAAGGAT GGTGGTGGCC
    AGTGACAAAA GCGGGCAGCC TGTGACAGCA GATGATTTGG GGGTGACAGG TGCTTTGACA
    GTTTTGATGA AAGATGCAAT AAAACCAAAC CTGATGCAGA CCCTGGAAGG GACACCTGTG
    TTCGTGCATG CGGGCCCTTT TGCTAACATT GCTCACGGCA ACTCTTCAGT GTTGGCTGAT
    AAAATTGCCC TGAAACTGGT TGGTGAAGAA GGATTTGTAG TGACCGAAGC TGGCTTTGGT
    GCTGACATCG GAATGGAGAA ATTCTTCAAC ATCAAGTGCC GAGCTTCCGG CTTGGTGCCC
    AACGTGGTTG TGTTAGTGGC AACGGTGCGA GCTCTGAAGA TGCATGGAGG CGGGCCAAGT
    GTAACGGCTG GTGTTCCTCT TAAGAAAGAA TATACAGAGG AGAACATCCA GCTGGTGGCA
    GACGGCTGCT GTAACCTCCA GAAGCAAATT CAGATCACTC AGCTCTTTGG GGTTCCCGTT
    GTGGTGGCTC TGAATGTCTT CAAGACCGAC ACCCGCGCTG AGATTGACTT GGTGTGTGAG
    CTTGCAAAGC GGGCTGGTGC CTTTGATGCA GTCCCCTGCT ATCACTGGTC CGTTGGTGGA
    AAAGGATCGG TGGACTTGGC TCGGGCTGTG AGAGAGGCTG CGAGTAAAAG AAGCCGATTC
    CAGTTCCTGT ATGATGTTCA GGTTCCAATT GTGGACAAGA TAAGGACCAT TGCTCAGGCT
    GTCTATGGAG CCAAAGATAT TGAACTCTCT CCTGAGGCAC AAGCCAAAAT AGATCGTTAC
    ACTCAACAGG GTTTTGGAAA TTTGCCCATC TGCATGGCAA AGACCCACCT TTCTCTATCT
    CACCAACCTG ACAAAAAAGG TGTGCCAAGG GACTTCATCT TACCTATCAG TGACGTCCGG
    GCCAGCATAG GCGCTGGGTT CATTTACCCT TTGGTCGGAA CGATGAGCAC CATGCCAGGA
    CTGCCCACCC GGCCCTGCTT TTATGACATA GATCTTGATA CCGAAACAGA ACAAGTTAAA
    GGCTTGTTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06995
    Clone ID Related Accession (Same CDS sequence) NM_015440.4
    Accession Version NM_015440.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2937bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-03
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB727926.1, AY374130.1 and BC110319.1. On Jun 24, 2011 this sequence version replaced NM_015440.3. Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY374130.1, SRR1803616.267860.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGGTGTCAA CCTAGATGGA
    AAGAAGATTT TGGTAGTGGG GGCCCATGGG TCTTTGGAAG CTGCTCTACA ATGCCTGTTC
    CAGAGAAAAG GGTCCATGAC AATGAGCATC CAGTGGAAAA CACGCCAGCT TCAAAGCAAG
    CTTCACGAGG CTGACATTGT GGTCCTAGGC TCACCTAAGC CAGAAGAGAT TCCCCTTACT
    TGGATACAAC CAGGAACTAC TGTTCTCAAC TGCTCCCATG ACTTCCTGTC AGGGAAGGTT
    GGGTGTGGCT CTCCAAGAAT ACATTTTGGT GGACTCATTG AGGAAGATGA TGTGATTCTC
    CTTGCTGCAG CTCTGCGAAT TCAGAACATG GTCAGTAGTG GAAGGAGATG GCTTCGTGAA
    CAGCAGCACA GGCGGTGGAG ACTTCACTGC TTGAAACTTC AGCCTCTCTC CCCTGTGCCA
    AGTGACATTG AGATTTCAAG AGGACAAACT CCAAAAGCTG TGGATGTCCT TGCCAAGGAG
    ATTGGATTGC TTGCAGATGA AATTGAAATC TATGGCAAAA GCAAAGCCAA AGTACGTTTG
    TCCGTGCTAG AAAGGTTAAA GGATCAAGCA GATGGAAAAT ACGTCTTAGT TGCTGGGATC
    ACACCCACCC CTCTTGGAGA AGGGAAGAGC ACAGTCACCA TCGGGCTTGT GCAGGCTCTG
    ACCGCACACC TGAATGTCAA CTCCTTTGCC TGCTTGAGGC AGCCTTCCCA AGGACCGACG
    TTTGGAGTGA AAGGAGGAGC CGCGGGTGGT GGATATGCCC AGGTCATCCC CATGGAGGAG
    TTCAACCTTC ACTTGACTGG AGACATCCAC GCCATCACCG CTGCCAATAA CTTGCTGGCT
    GCCGCCATCG ACACGAGGAT TCTTCATGAA AACACGCAAA CAGATAAGGC TCTGTATAAT
    CGGCTGGTTC CTTTAGTGAA TGGTGTCAGA GAATTTTCAG AAATTCAGCT TGCTCGGCTA
    AAAAAACTGG GAATAAATAA GACTGATCCG AGCACACTGA CAGAAGAGGA AGTGAGTAAA
    TTTGCCCGTC TCGACATCGA CCCATCTACC ATCACGTGGC AGAGAGTATT GGATACAAAT
    GACCGATTTC TACGAAAAAT AACCATCGGG CAGGGAAACA CAGAGAAGGG CCATTACCGG
    CAGGCGCAGT TTGACATCGC AGTGGCCAGC GAGATCATGG CGGTGCTGGC CCTGACGGAC
    AGCCTCGCAG ACATGAAGGC ACGGCTGGGA AGGATGGTGG TGGCCAGTGA CAAAAGCGGG
    CAGCCTGTGA CAGCAGATGA TTTGGGGGTG ACAGGTGCTT TGACAGTTTT GATGAAAGAT
    GCAATAAAAC CAAACCTGAT GCAGACCCTG GAAGGGACAC CTGTGTTCGT GCATGCGGGC
    CCTTTTGCTA ACATTGCTCA CGGCAACTCT TCAGTGTTGG CTGATAAAAT TGCCCTGAAA
    CTGGTTGGTG AAGAAGGATT TGTAGTGACC GAAGCTGGCT TTGGTGCTGA CATCGGAATG
    GAGAAATTCT TCAACATCAA GTGCCGAGCT TCCGGCTTGG TGCCCAACGT GGTTGTGTTA
    GTGGCAACGG TGCGAGCTCT GAAGATGCAT GGAGGCGGGC CAAGTGTAAC GGCTGGTGTT
    CCTCTTAAGA AAGAATATAC AGAGGAGAAC ATCCAGCTGG TGGCAGACGG CTGCTGTAAC
    CTCCAGAAGC AAATTCAGAT CACTCAGCTC TTTGGGGTTC CCGTTGTGGT GGCTCTGAAT
    GTCTTCAAGA CCGACACCCG CGCTGAGATT GACTTGGTGT GTGAGCTTGC AAAGCGGGCT
    GGTGCCTTTG ATGCAGTCCC CTGCTATCAC TGGTCCGTTG GTGGAAAAGG ATCGGTGGAC
    TTGGCTCGGG CTGTGAGAGA GGCTGCGAGT AAAAGAAGCC GATTCCAGTT CCTGTATGAT
    GTTCAGGTTC CAATTGTGGA CAAGATAAGG ACCATTGCTC AGGCTGTCTA TGGAGCCAAA
    GATATTGAAC TCTCTCCTGA GGCACAAGCC AAAATAGATC GTTACACTCA ACAGGGTTTT
    GGAAATTTGC CCATCTGCAT GGCAAAGACC CACCTTTCTC TATCTCACCA ACCTGACAAA
    AAAGGTGTGC CAAGGGACTT CATCTTACCT ATCAGTGACG TCCGGGCCAG CATAGGCGCT
    GGGTTCATTT ACCCTTTGGT CGGAACGATG AGCACCATGC CAGGACTGCC CACCCGGCCC
    TGCTTTTATG ACATAGATCT TGATACCGAA ACAGAACAAG TTAAAGGCTT GTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_056255.2
    CDS145..3081
    Translation

    Target ORF information:

    RefSeq Version NM_015440.4
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_015440.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGGTGTCAA CCTAGATGGA
    AAGAAGATTT TGGTAGTGGG GGCCCATGGG TCTTTGGAAG CTGCTCTACA ATGCCTGTTC
    CAGAGAAAAG GGTCCATGAC AATGAGCATC CAGTGGAAAA CACGCCAGCT TCAAAGCAAG
    CTTCACGAGG CTGACATTGT GGTCCTAGGC TCACCTAAGC CAGAAGAGAT TCCCCTTACT
    TGGATACAAC CAGGAACTAC TGTTCTCAAC TGCTCCCATG ACTTCCTGTC AGGGAAGGTT
    GGGTGTGGCT CTCCAAGAAT ACATTTTGGT GGACTCATTG AGGAAGATGA TGTGATTCTC
    CTTGCTGCAG CTCTGCGAAT TCAGAACATG GTCAGTAGTG GAAGGAGATG GCTTCGTGAA
    CAGCAGCACA GGCGGTGGAG ACTTCACTGC TTGAAACTTC AGCCTCTCTC CCCTGTGCCA
    AGTGACATTG AGATTTCAAG AGGACAAACT CCAAAAGCTG TGGATGTCCT TGCCAAGGAG
    ATTGGATTGC TTGCAGATGA AATTGAAATC TATGGCAAAA GCAAAGCCAA AGTACGTTTG
    TCCGTGCTAG AAAGGTTAAA GGATCAAGCA GATGGAAAAT ACGTCTTAGT TGCTGGGATC
    ACACCCACCC CTCTTGGAGA AGGGAAGAGC ACAGTCACCA TCGGGCTTGT GCAGGCTCTG
    ACCGCACACC TGAATGTCAA CTCCTTTGCC TGCTTGAGGC AGCCTTCCCA AGGACCGACG
    TTTGGAGTGA AAGGAGGAGC CGCGGGTGGT GGATATGCCC AGGTCATCCC CATGGAGGAG
    TTCAACCTTC ACTTGACTGG AGACATCCAC GCCATCACCG CTGCCAATAA CTTGCTGGCT
    GCCGCCATCG ACACGAGGAT TCTTCATGAA AACACGCAAA CAGATAAGGC TCTGTATAAT
    CGGCTGGTTC CTTTAGTGAA TGGTGTCAGA GAATTTTCAG AAATTCAGCT TGCTCGGCTA
    AAAAAACTGG GAATAAATAA GACTGATCCG AGCACACTGA CAGAAGAGGA AGTGAGTAAA
    TTTGCCCGTC TCGACATCGA CCCATCTACC ATCACGTGGC AGAGAGTATT GGATACAAAT
    GACCGATTTC TACGAAAAAT AACCATCGGG CAGGGAAACA CAGAGAAGGG CCATTACCGG
    CAGGCGCAGT TTGACATCGC AGTGGCCAGC GAGATCATGG CGGTGCTGGC CCTGACGGAC
    AGCCTCGCAG ACATGAAGGC ACGGCTGGGA AGGATGGTGG TGGCCAGTGA CAAAAGCGGG
    CAGCCTGTGA CAGCAGATGA TTTGGGGGTG ACAGGTGCTT TGACAGTTTT GATGAAAGAT
    GCAATAAAAC CAAACCTGAT GCAGACCCTG GAAGGGACAC CTGTGTTCGT GCATGCGGGC
    CCTTTTGCTA ACATTGCTCA CGGCAACTCT TCAGTGTTGG CTGATAAAAT TGCCCTGAAA
    CTGGTTGGTG AAGAAGGATT TGTAGTGACC GAAGCTGGCT TTGGTGCTGA CATCGGAATG
    GAGAAATTCT TCAACATCAA GTGCCGAGCT TCCGGCTTGG TGCCCAACGT GGTTGTGTTA
    GTGGCAACGG TGCGAGCTCT GAAGATGCAT GGAGGCGGGC CAAGTGTAAC GGCTGGTGTT
    CCTCTTAAGA AAGAATATAC AGAGGAGAAC ATCCAGCTGG TGGCAGACGG CTGCTGTAAC
    CTCCAGAAGC AAATTCAGAT CACTCAGCTC TTTGGGGTTC CCGTTGTGGT GGCTCTGAAT
    GTCTTCAAGA CCGACACCCG CGCTGAGATT GACTTGGTGT GTGAGCTTGC AAAGCGGGCT
    GGTGCCTTTG ATGCAGTCCC CTGCTATCAC TGGTCCGTTG GTGGAAAAGG ATCGGTGGAC
    TTGGCTCGGG CTGTGAGAGA GGCTGCGAGT AAAAGAAGCC GATTCCAGTT CCTGTATGAT
    GTTCAGGTTC CAATTGTGGA CAAGATAAGG ACCATTGCTC AGGCTGTCTA TGGAGCCAAA
    GATATTGAAC TCTCTCCTGA GGCACAAGCC AAAATAGATC GTTACACTCA ACAGGGTTTT
    GGAAATTTGC CCATCTGCAT GGCAAAGACC CACCTTTCTC TATCTCACCA ACCTGACAAA
    AAAGGTGTGC CAAGGGACTT CATCTTACCT ATCAGTGACG TCCGGGCCAG CATAGGCGCT
    GGGTTCATTT ACCCTTTGGT CGGAACGATG AGCACCATGC CAGGACTGCC CACCCGGCCC
    TGCTTTTATG ACATAGATCT TGATACCGAA ACAGAACAAG TTAAAGGCTT GTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu07104
    Clone ID Related Accession (Same CDS sequence) NM_001242769.1 , NM_001242769.2
    Accession Version NM_001242769.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform 4 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB727926.1, AY374131.1 and AW593866.1. Transcript Variant: This variant (4) differs in the 3' UTR and coding sequence and uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (4) has a shorter and distinct C-terminus and lacks a single internal aa compared to isoform 1. Isoform 4 is thought to lack THF synthase activity. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.408805.1, SRR1163658.302871.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGGTGTCAA CCTAGATGGA
    AAGAAGATTT TGGTAGTGGG GGCCCATGGG TCTTTGGAAG CTGCTCTACA ATGCCTGTTC
    CAGAGAAAAG GGTCCATGAC AATGAGCATC CAGTGGAAAA CACGCCAGCT TCAAAGCAAG
    ACGGAGTCTC GTTCTGTCAC CAGGCTGGAG TGCAGGCGCG TGATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001229698.1
    CDS145..972
    Translation

    Target ORF information:

    RefSeq Version NM_001242769.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001242769.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGGTGTCAA CCTAGATGGA
    AAGAAGATTT TGGTAGTGGG GGCCCATGGG TCTTTGGAAG CTGCTCTACA ATGCCTGTTC
    CAGAGAAAAG GGTCCATGAC AATGAGCATC CAGTGGAAAA CACGCCAGCT TCAAAGCAAG
    ACGGAGTCTC GTTCTGTCAC CAGGCTGGAG TGCAGGCGCG TGATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu07104
    Clone ID Related Accession (Same CDS sequence) NM_001242769.1 , NM_001242769.2
    Accession Version NM_001242769.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-01
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform 4 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB727926.1, AY374131.1, AW593866.1 and AL035086.12. On Jun 2, 2019 this sequence version replaced NM_001242769.1. Transcript Variant: This variant (4) differs in the 3' UTR and coding sequence and uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (4) has a shorter and distinct C-terminus and lacks a single internal aa compared to isoform 1. Isoform 4 is thought to lack THF synthase activity. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY374131.1, SRR1163657.538931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGGTGTCAA CCTAGATGGA
    AAGAAGATTT TGGTAGTGGG GGCCCATGGG TCTTTGGAAG CTGCTCTACA ATGCCTGTTC
    CAGAGAAAAG GGTCCATGAC AATGAGCATC CAGTGGAAAA CACGCCAGCT TCAAAGCAAG
    ACGGAGTCTC GTTCTGTCAC CAGGCTGGAG TGCAGGCGCG TGATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001229698.1
    CDS122..949
    Translation

    Target ORF information:

    RefSeq Version NM_001242769.2
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001242769.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGGTGTCAA CCTAGATGGA
    AAGAAGATTT TGGTAGTGGG GGCCCATGGG TCTTTGGAAG CTGCTCTACA ATGCCTGTTC
    CAGAGAAAAG GGTCCATGAC AATGAGCATC CAGTGGAAAA CACGCCAGCT TCAAAGCAAG
    ACGGAGTCTC GTTCTGTCAC CAGGCTGGAG TGCAGGCGCG TGATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101348
    Clone ID Related Accession (Same CDS sequence) NM_001350489.2 , NM_001350489.1
    Accession Version NM_001350489.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 726bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-17
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform 8 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL035086.12. On Jun 2, 2019 this sequence version replaced NM_001350489.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ219588.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGACGGAGT CTCGTTCTGT
    CACCAGGCTG GAGTGCAGGC GCGTGATCTA GGCTCACTGC AAGCTCTGCC TCCCAGGTTG
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337418.1
    CDS122..847
    Translation

    Target ORF information:

    RefSeq Version NM_001350489.2
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350489.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGACGGAGT CTCGTTCTGT
    CACCAGGCTG GAGTGCAGGC GCGTGATCTA GGCTCACTGC AAGCTCTGCC TCCCAGGTTG
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101348
    Clone ID Related Accession (Same CDS sequence) NM_001350489.2 , NM_001350489.1
    Accession Version NM_001350489.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 726bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform 8 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL035086.12. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ219588.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGACGGAGT CTCGTTCTGT
    CACCAGGCTG GAGTGCAGGC GCGTGATCTA GGCTCACTGC AAGCTCTGCC TCCCAGGTTG
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337418.1
    CDS145..870
    Translation

    Target ORF information:

    RefSeq Version NM_001350489.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350489.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGACGGAGT CTCGTTCTGT
    CACCAGGCTG GAGTGCAGGC GCGTGATCTA GGCTCACTGC AAGCTCTGCC TCCCAGGTTG
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101364
    Clone ID Related Accession (Same CDS sequence) NM_001350488.1 , NM_001350488.2
    Accession Version NM_001350488.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform 7 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL035086.12. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.146162.1, SRR1163658.430392.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGACGGAGT CTCGTTCTGT CACCAGGCTG GAGTGCAGGC GCGTGATCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337417.1
    CDS145..975
    Translation

    Target ORF information:

    RefSeq Version NM_001350488.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350488.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGACGGAGT CTCGTTCTGT CACCAGGCTG GAGTGCAGGC GCGTGATCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101364
    Clone ID Related Accession (Same CDS sequence) NM_001350488.1 , NM_001350488.2
    Accession Version NM_001350488.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-26
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform 7 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL035086.12. On Jun 2, 2019 this sequence version replaced NM_001350488.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.430392.1, SRR1163658.82894.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGACGGAGT CTCGTTCTGT CACCAGGCTG GAGTGCAGGC GCGTGATCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337417.1
    CDS122..952
    Translation

    Target ORF information:

    RefSeq Version NM_001350488.2
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350488.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGACGGAGT CTCGTTCTGT CACCAGGCTG GAGTGCAGGC GCGTGATCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40761
    Clone ID Related Accession (Same CDS sequence) XM_005266907.5 , XM_005266907.4
    Accession Version XM_005266907.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005266907.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGGTGTCAA CCTAGATGGA
    AAGAAGATTT TGGTAGTGGG GGCCCATGGG TCTTTGGAAG CTGCTCTACA ATGCCTGTTC
    CAGAGAAAAG GGTCCATGAC AATGAGCATC CAGTGGAAAA CACGCCAGCT TCAAAGCAAG
    CTTCACGAGG CTGACATTGT GGTCCTAGGC TCACCTAAGC CAGAAGAGAT TCCCCTTACT
    TGGATACAAC CAGGAACTAC TGTTCTCAAC TGCTCCCATG ACTTCCTGTC AGGGAAGGTT
    GGGTGTGGCT CTCCAAGAAT ACATTTTGGT GGACTCATTG AGGAAGATGA TGTGATTCTC
    CTTGCTGCAG CTCTGCGAAT TCAGAACATG GTCAGTAGTG GAAGGAGATG GCTTCGTGAA
    CAGCAGCACA GGCGGTGGAG ACTTCACTGC TTGAAACTTC AGCCTCTCTC CCCTGTGCCA
    AGTGACATTG AGATTTCAAG AGGACAAACT CCAAAAGCTG TGGATGTCCT TGCCAAGGAG
    ATTGGATTGC TTGCAGATGA AATTGAAATC TATGGCAAAA GCAAAGCCAA AGTACGTTTG
    TCCGTGCTAG AAAGGTTAAA GGATCAAGCA GATGGAAAAT ACGTCTTAGT TGCTGGGATC
    ACACCCACCC CTCTTGGAGA AGGGAAGAGC ACAGTCACCA TCGGGCTTGT GCAGGCTCTG
    ACCGCACACC TGAATGTCAA CTCCTTTGCC TGCTTGAGGC AGCCTTCCCA AGGACCGACG
    TTTGGAGTGA AAGGAGGAGC CGCGGGTGGT GGATATGCCC AGGTCATCCC CATGGAGGAG
    TTCAACCTTC ACTTGACTGG AGACATCCAC GCCATCACCG CTGCCAATAA CTTGCTGGCT
    GCCGCCATCG ACACGAGGAT TCTTCATGAA AACACGCAAA CAGATAAGGC TCTGTATAAT
    CGGCTGGTTC CTTTAGTGAA TGGTGTCAGA GAATTTTCAG AAATTCAGCT TGCTCGGCTA
    AAAAAACTGG GAATAAATAA GACTGATCCG AGCACACTGA CAGAAGAGGA AGTGAGTAAA
    TTTGCCCGTC TCGACATCGA CCCATCTACC ATCACGTGGC AGAGAGTATT GGATACAAAT
    GACCGATTTC TACGAAAAAT AACCATCGGG CAGGGAAACA CAGAGAAGGG CCATTACCGG
    CAGGCGCAGT TTGACATCGC AGTGGCCAGC GAGATCATGG CGGTGCTGGC CCTGACGGAC
    AGCCTCGCAG ACATGAAGGC ACGGCTGGGA AGGATGGTGG TGGCCAGTGA CAAAAGCGGG
    CAGCCTGTGA CAGCAGATGA TTTGGGGGTG ACAGGTGCTT TGACAGTTTT GATGAAAGAT
    GCAATAAAAC CAAACCTGAT GCAGACCCTG GAAGGGACAC CTGTGTTCGT GCATGCGGGC
    CCTTTTGCTA ACATTGCTCA CGGCAACTCT TCAGTGTTGG CTGATAAAAT TGCCCTGAAA
    CTGGTTGGTG AAGAAGGATT TGTAGTGACC GAAGCTGGCT TTGGTGCTGA CATCGGAATG
    GAGAAATTCT TCAACATCAA GTGCCGAGCT TCCGGCTTGG TGCCCAACGT GGTTGTGTTA
    GTGGCAACGG TGCGAGCTCT GAAGATGCAT GGAGGCGGGC CAAGTGTAAC GGCTGGTGTT
    CCTCTTAAGA AAGAATATAC AGAGGAGAAC ATCCAGCTGG TGGCAGACGG CTGCTGTAAC
    CTCCAGAAGC AAATTCAGAT CACTCAGCTC TTTGGGGTTC CCGTTGTGGT GGCTCTGAAT
    GTCTTCAAGA CCGACACCCG CGCTGAGATT GACTTGGTGT GTGAGCTTGC AAAGCGGGCT
    GGTGCCTTTG ATGCAGTCCC CTGCTATCAC TGGTCCGTTG GTGGAAAAGG ATCGGTGGAC
    TTGGCTCGGG CTGTGAGAGA GGCTGCGAGT AAAAGAAGCC GATTCCAGTT CCTGTATGAT
    GTTCAGGTTC CAATTGTGGA CAAGATAAGG ACCATTGCTC AGGCTGTCTA TGGAGCCAAA
    GATATTGAAC TCTCTCCTGA GGCACAAGCC AAAATAGATC GTTACACTCA ACAGGGTTTT
    GGAAATTTGC CCATCTGCAT GGCAAAGACC CACCTTTCTC TATCTCACCA ACCTGACAAA
    AAAGGTGTGC CAAGGGACTT CATCTTACCT ATCAGTGACG TCCGGGCCAG CATAGGCGCT
    GGGTTCATTT ACCCTTTGGT CGGAACGGCA AACCTGCTCA TGTTCTTCGC TGAAATCAAC
    CTCCCCTTTC TGGAATGGAC ATATGGAAAA TTGGAGGCAA AATATAATAA TGGTGGTGAC
    TCAGACTGCT TTAAGGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005266964.1
    CDS140..3100
    Translation

    Target ORF information:

    RefSeq Version XM_005266907.5
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005266907.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGGTGTCAA CCTAGATGGA
    AAGAAGATTT TGGTAGTGGG GGCCCATGGG TCTTTGGAAG CTGCTCTACA ATGCCTGTTC
    CAGAGAAAAG GGTCCATGAC AATGAGCATC CAGTGGAAAA CACGCCAGCT TCAAAGCAAG
    CTTCACGAGG CTGACATTGT GGTCCTAGGC TCACCTAAGC CAGAAGAGAT TCCCCTTACT
    TGGATACAAC CAGGAACTAC TGTTCTCAAC TGCTCCCATG ACTTCCTGTC AGGGAAGGTT
    GGGTGTGGCT CTCCAAGAAT ACATTTTGGT GGACTCATTG AGGAAGATGA TGTGATTCTC
    CTTGCTGCAG CTCTGCGAAT TCAGAACATG GTCAGTAGTG GAAGGAGATG GCTTCGTGAA
    CAGCAGCACA GGCGGTGGAG ACTTCACTGC TTGAAACTTC AGCCTCTCTC CCCTGTGCCA
    AGTGACATTG AGATTTCAAG AGGACAAACT CCAAAAGCTG TGGATGTCCT TGCCAAGGAG
    ATTGGATTGC TTGCAGATGA AATTGAAATC TATGGCAAAA GCAAAGCCAA AGTACGTTTG
    TCCGTGCTAG AAAGGTTAAA GGATCAAGCA GATGGAAAAT ACGTCTTAGT TGCTGGGATC
    ACACCCACCC CTCTTGGAGA AGGGAAGAGC ACAGTCACCA TCGGGCTTGT GCAGGCTCTG
    ACCGCACACC TGAATGTCAA CTCCTTTGCC TGCTTGAGGC AGCCTTCCCA AGGACCGACG
    TTTGGAGTGA AAGGAGGAGC CGCGGGTGGT GGATATGCCC AGGTCATCCC CATGGAGGAG
    TTCAACCTTC ACTTGACTGG AGACATCCAC GCCATCACCG CTGCCAATAA CTTGCTGGCT
    GCCGCCATCG ACACGAGGAT TCTTCATGAA AACACGCAAA CAGATAAGGC TCTGTATAAT
    CGGCTGGTTC CTTTAGTGAA TGGTGTCAGA GAATTTTCAG AAATTCAGCT TGCTCGGCTA
    AAAAAACTGG GAATAAATAA GACTGATCCG AGCACACTGA CAGAAGAGGA AGTGAGTAAA
    TTTGCCCGTC TCGACATCGA CCCATCTACC ATCACGTGGC AGAGAGTATT GGATACAAAT
    GACCGATTTC TACGAAAAAT AACCATCGGG CAGGGAAACA CAGAGAAGGG CCATTACCGG
    CAGGCGCAGT TTGACATCGC AGTGGCCAGC GAGATCATGG CGGTGCTGGC CCTGACGGAC
    AGCCTCGCAG ACATGAAGGC ACGGCTGGGA AGGATGGTGG TGGCCAGTGA CAAAAGCGGG
    CAGCCTGTGA CAGCAGATGA TTTGGGGGTG ACAGGTGCTT TGACAGTTTT GATGAAAGAT
    GCAATAAAAC CAAACCTGAT GCAGACCCTG GAAGGGACAC CTGTGTTCGT GCATGCGGGC
    CCTTTTGCTA ACATTGCTCA CGGCAACTCT TCAGTGTTGG CTGATAAAAT TGCCCTGAAA
    CTGGTTGGTG AAGAAGGATT TGTAGTGACC GAAGCTGGCT TTGGTGCTGA CATCGGAATG
    GAGAAATTCT TCAACATCAA GTGCCGAGCT TCCGGCTTGG TGCCCAACGT GGTTGTGTTA
    GTGGCAACGG TGCGAGCTCT GAAGATGCAT GGAGGCGGGC CAAGTGTAAC GGCTGGTGTT
    CCTCTTAAGA AAGAATATAC AGAGGAGAAC ATCCAGCTGG TGGCAGACGG CTGCTGTAAC
    CTCCAGAAGC AAATTCAGAT CACTCAGCTC TTTGGGGTTC CCGTTGTGGT GGCTCTGAAT
    GTCTTCAAGA CCGACACCCG CGCTGAGATT GACTTGGTGT GTGAGCTTGC AAAGCGGGCT
    GGTGCCTTTG ATGCAGTCCC CTGCTATCAC TGGTCCGTTG GTGGAAAAGG ATCGGTGGAC
    TTGGCTCGGG CTGTGAGAGA GGCTGCGAGT AAAAGAAGCC GATTCCAGTT CCTGTATGAT
    GTTCAGGTTC CAATTGTGGA CAAGATAAGG ACCATTGCTC AGGCTGTCTA TGGAGCCAAA
    GATATTGAAC TCTCTCCTGA GGCACAAGCC AAAATAGATC GTTACACTCA ACAGGGTTTT
    GGAAATTTGC CCATCTGCAT GGCAAAGACC CACCTTTCTC TATCTCACCA ACCTGACAAA
    AAAGGTGTGC CAAGGGACTT CATCTTACCT ATCAGTGACG TCCGGGCCAG CATAGGCGCT
    GGGTTCATTT ACCCTTTGGT CGGAACGGCA AACCTGCTCA TGTTCTTCGC TGAAATCAAC
    CTCCCCTTTC TGGAATGGAC ATATGGAAAA TTGGAGGCAA AATATAATAA TGGTGGTGAC
    TCAGACTGCT TTAAGGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40761
    Clone ID Related Accession (Same CDS sequence) XM_005266907.5 , XM_005266907.4
    Accession Version XM_005266907.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005266907.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGGTGTCAA CCTAGATGGA
    AAGAAGATTT TGGTAGTGGG GGCCCATGGG TCTTTGGAAG CTGCTCTACA ATGCCTGTTC
    CAGAGAAAAG GGTCCATGAC AATGAGCATC CAGTGGAAAA CACGCCAGCT TCAAAGCAAG
    CTTCACGAGG CTGACATTGT GGTCCTAGGC TCACCTAAGC CAGAAGAGAT TCCCCTTACT
    TGGATACAAC CAGGAACTAC TGTTCTCAAC TGCTCCCATG ACTTCCTGTC AGGGAAGGTT
    GGGTGTGGCT CTCCAAGAAT ACATTTTGGT GGACTCATTG AGGAAGATGA TGTGATTCTC
    CTTGCTGCAG CTCTGCGAAT TCAGAACATG GTCAGTAGTG GAAGGAGATG GCTTCGTGAA
    CAGCAGCACA GGCGGTGGAG ACTTCACTGC TTGAAACTTC AGCCTCTCTC CCCTGTGCCA
    AGTGACATTG AGATTTCAAG AGGACAAACT CCAAAAGCTG TGGATGTCCT TGCCAAGGAG
    ATTGGATTGC TTGCAGATGA AATTGAAATC TATGGCAAAA GCAAAGCCAA AGTACGTTTG
    TCCGTGCTAG AAAGGTTAAA GGATCAAGCA GATGGAAAAT ACGTCTTAGT TGCTGGGATC
    ACACCCACCC CTCTTGGAGA AGGGAAGAGC ACAGTCACCA TCGGGCTTGT GCAGGCTCTG
    ACCGCACACC TGAATGTCAA CTCCTTTGCC TGCTTGAGGC AGCCTTCCCA AGGACCGACG
    TTTGGAGTGA AAGGAGGAGC CGCGGGTGGT GGATATGCCC AGGTCATCCC CATGGAGGAG
    TTCAACCTTC ACTTGACTGG AGACATCCAC GCCATCACCG CTGCCAATAA CTTGCTGGCT
    GCCGCCATCG ACACGAGGAT TCTTCATGAA AACACGCAAA CAGATAAGGC TCTGTATAAT
    CGGCTGGTTC CTTTAGTGAA TGGTGTCAGA GAATTTTCAG AAATTCAGCT TGCTCGGCTA
    AAAAAACTGG GAATAAATAA GACTGATCCG AGCACACTGA CAGAAGAGGA AGTGAGTAAA
    TTTGCCCGTC TCGACATCGA CCCATCTACC ATCACGTGGC AGAGAGTATT GGATACAAAT
    GACCGATTTC TACGAAAAAT AACCATCGGG CAGGGAAACA CAGAGAAGGG CCATTACCGG
    CAGGCGCAGT TTGACATCGC AGTGGCCAGC GAGATCATGG CGGTGCTGGC CCTGACGGAC
    AGCCTCGCAG ACATGAAGGC ACGGCTGGGA AGGATGGTGG TGGCCAGTGA CAAAAGCGGG
    CAGCCTGTGA CAGCAGATGA TTTGGGGGTG ACAGGTGCTT TGACAGTTTT GATGAAAGAT
    GCAATAAAAC CAAACCTGAT GCAGACCCTG GAAGGGACAC CTGTGTTCGT GCATGCGGGC
    CCTTTTGCTA ACATTGCTCA CGGCAACTCT TCAGTGTTGG CTGATAAAAT TGCCCTGAAA
    CTGGTTGGTG AAGAAGGATT TGTAGTGACC GAAGCTGGCT TTGGTGCTGA CATCGGAATG
    GAGAAATTCT TCAACATCAA GTGCCGAGCT TCCGGCTTGG TGCCCAACGT GGTTGTGTTA
    GTGGCAACGG TGCGAGCTCT GAAGATGCAT GGAGGCGGGC CAAGTGTAAC GGCTGGTGTT
    CCTCTTAAGA AAGAATATAC AGAGGAGAAC ATCCAGCTGG TGGCAGACGG CTGCTGTAAC
    CTCCAGAAGC AAATTCAGAT CACTCAGCTC TTTGGGGTTC CCGTTGTGGT GGCTCTGAAT
    GTCTTCAAGA CCGACACCCG CGCTGAGATT GACTTGGTGT GTGAGCTTGC AAAGCGGGCT
    GGTGCCTTTG ATGCAGTCCC CTGCTATCAC TGGTCCGTTG GTGGAAAAGG ATCGGTGGAC
    TTGGCTCGGG CTGTGAGAGA GGCTGCGAGT AAAAGAAGCC GATTCCAGTT CCTGTATGAT
    GTTCAGGTTC CAATTGTGGA CAAGATAAGG ACCATTGCTC AGGCTGTCTA TGGAGCCAAA
    GATATTGAAC TCTCTCCTGA GGCACAAGCC AAAATAGATC GTTACACTCA ACAGGGTTTT
    GGAAATTTGC CCATCTGCAT GGCAAAGACC CACCTTTCTC TATCTCACCA ACCTGACAAA
    AAAGGTGTGC CAAGGGACTT CATCTTACCT ATCAGTGACG TCCGGGCCAG CATAGGCGCT
    GGGTTCATTT ACCCTTTGGT CGGAACGGCA AACCTGCTCA TGTTCTTCGC TGAAATCAAC
    CTCCCCTTTC TGGAATGGAC ATATGGAAAA TTGGAGGCAA AATATAATAA TGGTGGTGAC
    TCAGACTGCT TTAAGGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005266964.1
    CDS86..3046
    Translation

    Target ORF information:

    RefSeq Version XM_005266907.4
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005266907.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGGTGTCAA CCTAGATGGA
    AAGAAGATTT TGGTAGTGGG GGCCCATGGG TCTTTGGAAG CTGCTCTACA ATGCCTGTTC
    CAGAGAAAAG GGTCCATGAC AATGAGCATC CAGTGGAAAA CACGCCAGCT TCAAAGCAAG
    CTTCACGAGG CTGACATTGT GGTCCTAGGC TCACCTAAGC CAGAAGAGAT TCCCCTTACT
    TGGATACAAC CAGGAACTAC TGTTCTCAAC TGCTCCCATG ACTTCCTGTC AGGGAAGGTT
    GGGTGTGGCT CTCCAAGAAT ACATTTTGGT GGACTCATTG AGGAAGATGA TGTGATTCTC
    CTTGCTGCAG CTCTGCGAAT TCAGAACATG GTCAGTAGTG GAAGGAGATG GCTTCGTGAA
    CAGCAGCACA GGCGGTGGAG ACTTCACTGC TTGAAACTTC AGCCTCTCTC CCCTGTGCCA
    AGTGACATTG AGATTTCAAG AGGACAAACT CCAAAAGCTG TGGATGTCCT TGCCAAGGAG
    ATTGGATTGC TTGCAGATGA AATTGAAATC TATGGCAAAA GCAAAGCCAA AGTACGTTTG
    TCCGTGCTAG AAAGGTTAAA GGATCAAGCA GATGGAAAAT ACGTCTTAGT TGCTGGGATC
    ACACCCACCC CTCTTGGAGA AGGGAAGAGC ACAGTCACCA TCGGGCTTGT GCAGGCTCTG
    ACCGCACACC TGAATGTCAA CTCCTTTGCC TGCTTGAGGC AGCCTTCCCA AGGACCGACG
    TTTGGAGTGA AAGGAGGAGC CGCGGGTGGT GGATATGCCC AGGTCATCCC CATGGAGGAG
    TTCAACCTTC ACTTGACTGG AGACATCCAC GCCATCACCG CTGCCAATAA CTTGCTGGCT
    GCCGCCATCG ACACGAGGAT TCTTCATGAA AACACGCAAA CAGATAAGGC TCTGTATAAT
    CGGCTGGTTC CTTTAGTGAA TGGTGTCAGA GAATTTTCAG AAATTCAGCT TGCTCGGCTA
    AAAAAACTGG GAATAAATAA GACTGATCCG AGCACACTGA CAGAAGAGGA AGTGAGTAAA
    TTTGCCCGTC TCGACATCGA CCCATCTACC ATCACGTGGC AGAGAGTATT GGATACAAAT
    GACCGATTTC TACGAAAAAT AACCATCGGG CAGGGAAACA CAGAGAAGGG CCATTACCGG
    CAGGCGCAGT TTGACATCGC AGTGGCCAGC GAGATCATGG CGGTGCTGGC CCTGACGGAC
    AGCCTCGCAG ACATGAAGGC ACGGCTGGGA AGGATGGTGG TGGCCAGTGA CAAAAGCGGG
    CAGCCTGTGA CAGCAGATGA TTTGGGGGTG ACAGGTGCTT TGACAGTTTT GATGAAAGAT
    GCAATAAAAC CAAACCTGAT GCAGACCCTG GAAGGGACAC CTGTGTTCGT GCATGCGGGC
    CCTTTTGCTA ACATTGCTCA CGGCAACTCT TCAGTGTTGG CTGATAAAAT TGCCCTGAAA
    CTGGTTGGTG AAGAAGGATT TGTAGTGACC GAAGCTGGCT TTGGTGCTGA CATCGGAATG
    GAGAAATTCT TCAACATCAA GTGCCGAGCT TCCGGCTTGG TGCCCAACGT GGTTGTGTTA
    GTGGCAACGG TGCGAGCTCT GAAGATGCAT GGAGGCGGGC CAAGTGTAAC GGCTGGTGTT
    CCTCTTAAGA AAGAATATAC AGAGGAGAAC ATCCAGCTGG TGGCAGACGG CTGCTGTAAC
    CTCCAGAAGC AAATTCAGAT CACTCAGCTC TTTGGGGTTC CCGTTGTGGT GGCTCTGAAT
    GTCTTCAAGA CCGACACCCG CGCTGAGATT GACTTGGTGT GTGAGCTTGC AAAGCGGGCT
    GGTGCCTTTG ATGCAGTCCC CTGCTATCAC TGGTCCGTTG GTGGAAAAGG ATCGGTGGAC
    TTGGCTCGGG CTGTGAGAGA GGCTGCGAGT AAAAGAAGCC GATTCCAGTT CCTGTATGAT
    GTTCAGGTTC CAATTGTGGA CAAGATAAGG ACCATTGCTC AGGCTGTCTA TGGAGCCAAA
    GATATTGAAC TCTCTCCTGA GGCACAAGCC AAAATAGATC GTTACACTCA ACAGGGTTTT
    GGAAATTTGC CCATCTGCAT GGCAAAGACC CACCTTTCTC TATCTCACCA ACCTGACAAA
    AAAGGTGTGC CAAGGGACTT CATCTTACCT ATCAGTGACG TCCGGGCCAG CATAGGCGCT
    GGGTTCATTT ACCCTTTGGT CGGAACGGCA AACCTGCTCA TGTTCTTCGC TGAAATCAAC
    CTCCCCTTTC TGGAATGGAC ATATGGAAAA TTGGAGGCAA AATATAATAA TGGTGGTGAC
    TCAGACTGCT TTAAGGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40762
    Clone ID Related Accession (Same CDS sequence) XM_005266910.4
    Accession Version XM_005266910.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2964bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005266910.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGTG ACCGAAGCTG GCTTTGGTGC TGACATCGGA
    ATGGAGAAAT TCTTCAACAT CAAGTGCCGA GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG
    TTAGTGGCAA CGGTGCGAGC TCTGAAGATG CATGGAGGCG GGCCAAGTGT AACGGCTGGT
    GTTCCTCTTA AGAAAGAATA TACAGAGGAG AACATCCAGC TGGTGGCAGA CGGCTGCTGT
    AACCTCCAGA AGCAAATTCA GATCACTCAG CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG
    AATGTCTTCA AGACCGACAC CCGCGCTGAG ATTGACTTGG TGTGTGAGCT TGCAAAGCGG
    GCTGGTGCCT TTGATGCAGT CCCCTGCTAT CACTGGTCCG TTGGTGGAAA AGGATCGGTG
    GACTTGGCTC GGGCTGTGAG AGAGGCTGCG AGTAAAAGAA GCCGATTCCA GTTCCTGTAT
    GATGTTCAGG TTCCAATTGT GGACAAGATA AGGACCATTG CTCAGGCTGT CTATGGAGCC
    AAAGATATTG AACTCTCTCC TGAGGCACAA GCCAAAATAG ATCGTTACAC TCAACAGGGT
    TTTGGAAATT TGCCCATCTG CATGGCAAAG ACCCACCTTT CTCTATCTCA CCAACCTGAC
    AAAAAAGGTG TGCCAAGGGA CTTCATCTTA CCTATCAGTG ACGTCCGGGC CAGCATAGGC
    GCTGGGTTCA TTTACCCTTT GGTCGGAACG GCAAACCTGC TCATGTTCTT CGCTGAAATC
    AACCTCCCCT TTCTGGAATG GACATATGGA AAATTGGAGG CAAAATATAA TAATGGTGGT
    GACTCAGACT GCTTTAAGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005266967.1
    CDS86..3049
    Translation

    Target ORF information:

    RefSeq Version XM_005266910.4
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005266910.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGTG ACCGAAGCTG GCTTTGGTGC TGACATCGGA
    ATGGAGAAAT TCTTCAACAT CAAGTGCCGA GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG
    TTAGTGGCAA CGGTGCGAGC TCTGAAGATG CATGGAGGCG GGCCAAGTGT AACGGCTGGT
    GTTCCTCTTA AGAAAGAATA TACAGAGGAG AACATCCAGC TGGTGGCAGA CGGCTGCTGT
    AACCTCCAGA AGCAAATTCA GATCACTCAG CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG
    AATGTCTTCA AGACCGACAC CCGCGCTGAG ATTGACTTGG TGTGTGAGCT TGCAAAGCGG
    GCTGGTGCCT TTGATGCAGT CCCCTGCTAT CACTGGTCCG TTGGTGGAAA AGGATCGGTG
    GACTTGGCTC GGGCTGTGAG AGAGGCTGCG AGTAAAAGAA GCCGATTCCA GTTCCTGTAT
    GATGTTCAGG TTCCAATTGT GGACAAGATA AGGACCATTG CTCAGGCTGT CTATGGAGCC
    AAAGATATTG AACTCTCTCC TGAGGCACAA GCCAAAATAG ATCGTTACAC TCAACAGGGT
    TTTGGAAATT TGCCCATCTG CATGGCAAAG ACCCACCTTT CTCTATCTCA CCAACCTGAC
    AAAAAAGGTG TGCCAAGGGA CTTCATCTTA CCTATCAGTG ACGTCCGGGC CAGCATAGGC
    GCTGGGTTCA TTTACCCTTT GGTCGGAACG GCAAACCTGC TCATGTTCTT CGCTGAAATC
    AACCTCCCCT TTCTGGAATG GACATATGGA AAATTGGAGG CAAAATATAA TAATGGTGGT
    GACTCAGACT GCTTTAAGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40763
    Clone ID Related Accession (Same CDS sequence) XM_005266911.4 , XM_005266911.5
    Accession Version XM_005266911.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005266911.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGTAA TATTTTTTCA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005266968.1
    CDS86..1909
    Translation

    Target ORF information:

    RefSeq Version XM_005266911.4
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005266911.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGTAA TATTTTTTCA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40763
    Clone ID Related Accession (Same CDS sequence) XM_005266911.4 , XM_005266911.5
    Accession Version XM_005266911.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005266911.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGTAA TATTTTTTCA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005266968.1
    CDS140..1963
    Translation

    Target ORF information:

    RefSeq Version XM_005266911.5
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005266911.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGTAA TATTTTTTCA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66608
    Clone ID Related Accession (Same CDS sequence) XM_011535729.2 , XM_011535729.3
    Accession Version XM_011535729.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2868bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535729.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGTG ACCGAAGCTG GCTTTGGTGC TGACATCGGA
    ATGGAGAAAT TCTTCAACAT CAAGTGCCGA GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG
    TTAGTGGCAA CGGTGCGAGC TCTGAAGATG CATGGAGGCG GGCCAAGTGT AACGGCTGGT
    GTTCCTCTTA AGAAAGAATA TACAGAGGAG AACATCCAGC TGGTGGCAGA CGGCTGCTGT
    AACCTCCAGA AGCAAATTCA GATCACTCAG CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG
    AATGTCTTCA AGACCGACAC CCGCGCTGAG ATTGACTTGG TGTGTGAGCT TGCAAAGCGG
    GCTGGTGCCT TTGATGCAGT CCCCTGCTAT CACTGGTCCG TTGGTGGAAA AGGATCGGTG
    GACTTGGCTC GGGCTGTGAG AGAGGCTGCG AGTAAAAGAA GCCGATTCCA GTTCCTGTAT
    GATGTTCAGG TTCCAATTGT GGACAAGATA AGGACCATTG CTCAGGCTGT CTATGGAGCC
    AAAGATATTG AACTCTCTCC TGAGGCACAA GCCAAAATAG ATCGTTACAC TCAACAGGGT
    TTTGGAAATT TGCCCATCTG CATGGCAAAG ACCCACCTTT CTCTATCTCA CCAACCTGAC
    AAAAAAGGTG TGCCAAGGGA CTTCATCTTA CCTATCAGTG ACGTCCGGGC CAGCATAGGC
    GCTGGGTTCA TTTACCCTTT GGTCGGAACG TCCTCACATC AATCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011534031.1
    CDS86..2953
    Translation

    Target ORF information:

    RefSeq Version XM_011535729.2
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011535729.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGTG ACCGAAGCTG GCTTTGGTGC TGACATCGGA
    ATGGAGAAAT TCTTCAACAT CAAGTGCCGA GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG
    TTAGTGGCAA CGGTGCGAGC TCTGAAGATG CATGGAGGCG GGCCAAGTGT AACGGCTGGT
    GTTCCTCTTA AGAAAGAATA TACAGAGGAG AACATCCAGC TGGTGGCAGA CGGCTGCTGT
    AACCTCCAGA AGCAAATTCA GATCACTCAG CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG
    AATGTCTTCA AGACCGACAC CCGCGCTGAG ATTGACTTGG TGTGTGAGCT TGCAAAGCGG
    GCTGGTGCCT TTGATGCAGT CCCCTGCTAT CACTGGTCCG TTGGTGGAAA AGGATCGGTG
    GACTTGGCTC GGGCTGTGAG AGAGGCTGCG AGTAAAAGAA GCCGATTCCA GTTCCTGTAT
    GATGTTCAGG TTCCAATTGT GGACAAGATA AGGACCATTG CTCAGGCTGT CTATGGAGCC
    AAAGATATTG AACTCTCTCC TGAGGCACAA GCCAAAATAG ATCGTTACAC TCAACAGGGT
    TTTGGAAATT TGCCCATCTG CATGGCAAAG ACCCACCTTT CTCTATCTCA CCAACCTGAC
    AAAAAAGGTG TGCCAAGGGA CTTCATCTTA CCTATCAGTG ACGTCCGGGC CAGCATAGGC
    GCTGGGTTCA TTTACCCTTT GGTCGGAACG TCCTCACATC AATCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66608
    Clone ID Related Accession (Same CDS sequence) XM_011535729.2 , XM_011535729.3
    Accession Version XM_011535729.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2868bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011535729.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGTG ACCGAAGCTG GCTTTGGTGC TGACATCGGA
    ATGGAGAAAT TCTTCAACAT CAAGTGCCGA GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG
    TTAGTGGCAA CGGTGCGAGC TCTGAAGATG CATGGAGGCG GGCCAAGTGT AACGGCTGGT
    GTTCCTCTTA AGAAAGAATA TACAGAGGAG AACATCCAGC TGGTGGCAGA CGGCTGCTGT
    AACCTCCAGA AGCAAATTCA GATCACTCAG CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG
    AATGTCTTCA AGACCGACAC CCGCGCTGAG ATTGACTTGG TGTGTGAGCT TGCAAAGCGG
    GCTGGTGCCT TTGATGCAGT CCCCTGCTAT CACTGGTCCG TTGGTGGAAA AGGATCGGTG
    GACTTGGCTC GGGCTGTGAG AGAGGCTGCG AGTAAAAGAA GCCGATTCCA GTTCCTGTAT
    GATGTTCAGG TTCCAATTGT GGACAAGATA AGGACCATTG CTCAGGCTGT CTATGGAGCC
    AAAGATATTG AACTCTCTCC TGAGGCACAA GCCAAAATAG ATCGTTACAC TCAACAGGGT
    TTTGGAAATT TGCCCATCTG CATGGCAAAG ACCCACCTTT CTCTATCTCA CCAACCTGAC
    AAAAAAGGTG TGCCAAGGGA CTTCATCTTA CCTATCAGTG ACGTCCGGGC CAGCATAGGC
    GCTGGGTTCA TTTACCCTTT GGTCGGAACG TCCTCACATC AATCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011534031.1
    CDS140..3007
    Translation

    Target ORF information:

    RefSeq Version XM_011535729.3
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011535729.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGTG ACCGAAGCTG GCTTTGGTGC TGACATCGGA
    ATGGAGAAAT TCTTCAACAT CAAGTGCCGA GCTTCCGGCT TGGTGCCCAA CGTGGTTGTG
    TTAGTGGCAA CGGTGCGAGC TCTGAAGATG CATGGAGGCG GGCCAAGTGT AACGGCTGGT
    GTTCCTCTTA AGAAAGAATA TACAGAGGAG AACATCCAGC TGGTGGCAGA CGGCTGCTGT
    AACCTCCAGA AGCAAATTCA GATCACTCAG CTCTTTGGGG TTCCCGTTGT GGTGGCTCTG
    AATGTCTTCA AGACCGACAC CCGCGCTGAG ATTGACTTGG TGTGTGAGCT TGCAAAGCGG
    GCTGGTGCCT TTGATGCAGT CCCCTGCTAT CACTGGTCCG TTGGTGGAAA AGGATCGGTG
    GACTTGGCTC GGGCTGTGAG AGAGGCTGCG AGTAAAAGAA GCCGATTCCA GTTCCTGTAT
    GATGTTCAGG TTCCAATTGT GGACAAGATA AGGACCATTG CTCAGGCTGT CTATGGAGCC
    AAAGATATTG AACTCTCTCC TGAGGCACAA GCCAAAATAG ATCGTTACAC TCAACAGGGT
    TTTGGAAATT TGCCCATCTG CATGGCAAAG ACCCACCTTT CTCTATCTCA CCAACCTGAC
    AAAAAAGGTG TGCCAAGGGA CTTCATCTTA CCTATCAGTG ACGTCCGGGC CAGCATAGGC
    GCTGGGTTCA TTTACCCTTT GGTCGGAACG TCCTCACATC AATCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66615
    Clone ID Related Accession (Same CDS sequence) XM_011535737.3 , XM_011535737.2
    Accession Version XM_011535737.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2139bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011535737.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGGC TTGTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011534039.1
    CDS140..2278
    Translation

    Target ORF information:

    RefSeq Version XM_011535737.3
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011535737.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGGC TTGTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66615
    Clone ID Related Accession (Same CDS sequence) XM_011535737.3 , XM_011535737.2
    Accession Version XM_011535737.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2139bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535737.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGGC TTGTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011534039.1
    CDS86..2224
    Translation

    Target ORF information:

    RefSeq Version XM_011535737.2
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011535737.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGGC TTGTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66616
    Clone ID Related Accession (Same CDS sequence) XM_011535738.3 , XM_011535738.2
    Accession Version XM_011535738.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2133bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011535738.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGGC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011534040.1
    CDS140..2272
    Translation

    Target ORF information:

    RefSeq Version XM_011535738.3
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011535738.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGGC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66616
    Clone ID Related Accession (Same CDS sequence) XM_011535738.3 , XM_011535738.2
    Accession Version XM_011535738.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2133bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535738.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGGC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011534040.1
    CDS86..2218
    Translation

    Target ORF information:

    RefSeq Version XM_011535738.2
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011535738.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGGC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96523
    Clone ID Related Accession (Same CDS sequence) XM_017010708.1
    Accession Version XM_017010708.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2259bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGGA ACTTGCCAAG GTGCACAGCT GGGAGGGATC
    CGGGGTCCCG TCCGTGTGCT AACCTGCAGG ACAGCGCCGC ATAATCCAGT ACTGGGGCCT
    TATTGTCCAG CCTTCCACCT CCAGGAGCCC ACTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866197.1
    CDS87..2345
    Translation

    Target ORF information:

    RefSeq Version XM_017010708.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010708.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCACGC GTCTGCCGCT CGTCCTGCGC CAGCTCCGCC GCCCGCCCCA GCCCCCGGGC 
    CCTCCGCGCC GCCTCCGTGT GCCCTGTCGC GCTAGCAGCG GCGGCGGCGG AGGCGGCGGC
    GGTGGCCGGG AGGGCCTGCT TGGACAGCGG CGGCCGCAGG ATGGCCAGGC CCGGAGCAGC
    TGCAGCCCCG GCGGCCGAAC GCCCGCGGCG CGGGACTCCA TCGTCAGAGA AGTCATTCAG
    AATTCAAAAG AAGTTCTAAG TTTATTGCAA GAAAAAAACC CTGCCTTCAA GCCGGTTCTT
    GCAATTATCC AGGCAGGTGA CGACAACTTG ATGCAGGAAA TCAACCAGAA TTTGGCTGAG
    GAGGCTGGTC TGAACATCAC TCACATTTGC CTCCCTCCAG ATAGCAGTGA AGCCGAGATT
    ATAGATGAAA TCTTAAAGAT CAATGAAGAT ACCAGAGTAC ATGGCCTTGC CCTTCAGATC
    TCTGAGAACT TGTTTAGCAA CAAAGTCCTC AATGCCTTGA AACCAGAAAA AGATGTGGAT
    GGAGTAACAG ACATAAACCT GGGGAAGCTG GTGCGAGGGG ATGCCCATGA ATGTTTTGTT
    TCACCTGTTG CCAAAGCTGT AATTGAACTT CTTGAAAAAT CAGTAGGTGT CAACCTAGAT
    GGAAAGAAGA TTTTGGTAGT GGGGGCCCAT GGGTCTTTGG AAGCTGCTCT ACAATGCCTG
    TTCCAGAGAA AAGGGTCCAT GACAATGAGC ATCCAGTGGA AAACACGCCA GCTTCAAAGC
    AAGCTTCACG AGGCTGACAT TGTGGTCCTA GGCTCACCTA AGCCAGAAGA GATTCCCCTT
    ACTTGGATAC AACCAGGAAC TACTGTTCTC AACTGCTCCC ATGACTTCCT GTCAGGGAAG
    GTTGGGTGTG GCTCTCCAAG AATACATTTT GGTGGACTCA TTGAGGAAGA TGATGTGATT
    CTCCTTGCTG CAGCTCTGCG AATTCAGAAC ATGGTCAGTA GTGGAAGGAG ATGGCTTCGT
    GAACAGCAGC ACAGGCGGTG GAGACTTCAC TGCTTGAAAC TTCAGCCTCT CTCCCCTGTG
    CCAAGTGACA TTGAGATTTC AAGAGGACAA ACTCCAAAAG CTGTGGATGT CCTTGCCAAG
    GAGATTGGAT TGCTTGCAGA TGAAATTGAA ATCTATGGCA AAAGCAAAGC CAAAGTACGT
    TTGTCCGTGC TAGAAAGGTT AAAGGATCAA GCAGATGGAA AATACGTCTT AGTTGCTGGG
    ATCACACCCA CCCCTCTTGG AGAAGGGAAG AGCACAGTCA CCATCGGGCT TGTGCAGGCT
    CTGACCGCAC ACCTGAATGT CAACTCCTTT GCCTGCTTGA GGCAGCCTTC CCAAGGACCG
    ACGTTTGGAG TGAAAGGAGG AGCCGCGGGT GGTGGATATG CCCAGGTCAT CCCCATGGAG
    GAGTTCAACC TTCACTTGAC TGGAGACATC CACGCCATCA CCGCTGCCAA TAACTTGCTG
    GCTGCCGCCA TCGACACGAG GATTCTTCAT GAAAACACGC AAACAGATAA GGCTCTGTAT
    AATCGGCTGG TTCCTTTAGT GAATGGTGTC AGAGAATTTT CAGAAATTCA GCTTGCTCGG
    CTAAAAAAAC TGGGAATAAA TAAGACTGAT CCGAGCACAC TGACAGAAGA GGAAGTGAGT
    AAATTTGCCC GTCTCGACAT CGACCCATCT ACCATCACGT GGCAGAGAGT ATTGGATACA
    AATGACCGAT TTCTACGAAA AATAACCATC GGGCAGGGAA ACACAGAGAA GGGCCATTAC
    CGGCAGGCGC AGTTTGACAT CGCAGTGGCC AGCGAGATCA TGGCGGTGCT GGCCCTGACG
    GACAGCCTCG CAGACATGAA GGCACGGCTG GGAAGGATGG TGGTGGCCAG TGACAAAAGC
    GGGCAGCCTG TGACAGCAGA TGATTTGGGG GTGACAGGTG CTTTGACAGT TTTGATGAAA
    GATGCAATAA AACCAAACCT GATGCAGACC CTGGAAGGGA CACCTGTGTT CGTGCATGCG
    GGCCCTTTTG CTAACATTGC TCACGGCAAC TCTTCAGTGT TGGCTGATAA AATTGCCCTG
    AAACTGGTTG GTGAAGAAGG ATTTGTAGGA ACTTGCCAAG GTGCACAGCT GGGAGGGATC
    CGGGGTCCCG TCCGTGTGCT AACCTGCAGG ACAGCGCCGC ATAATCCAGT ACTGGGGCCT
    TATTGTCCAG CCTTCCACCT CCAGGAGCCC ACTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.