Ppm1e cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppm1e
Entrez Gene ID	360593
Full Name	protein phosphatase, Mg2+/Mn2+ dependent, 1E
General protein information	Preferred Names protein phosphatase, Mg2+/Mn2+ dependent, 1E Names protein phosphatase 1E ca(2+)/calmodulin-dependent protein kinase phosphatase N caMKN caMKP-N caMKP-nucleus calmodulin-dependent protein kinase phosphatase N partner of PIX 1 partner of PIX-alpha partner of PIXA protein phosphatase 1E (PP2C domain containing)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q26
Summary	serine/threonine phosphatase; deactivates the activated multifunctional CaM kinases [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_198773.1	NP_942068.1	protein phosphatase 1E

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ppm1e	NP_942068.1
Canis lupus familiaris (dog)	PPM1E	XP_853253.1
Gallus gallus (chicken)	PPM1E	XP_415871.4
Homo sapiens (human)	PPM1E	NP_055721.3
Danio rerio (zebrafish)	ppm1e	NP_001018354.1
Pan troglodytes (chimpanzee)	PPM1E	XP_523813.4
	LOC711012	XP_002800484.1
Bos taurus (cattle)	PPM1E	XP_002695648.3
Mus musculus (house mouse)	Ppm1e	NP_796141.2
Xenopus tropicalis (tropical clawed frog)	ppm1e	XP_002934392.1

Gene Ontology
Bibliography

Related articles in PubMed

N-Myristoylation is essential for protein phosphatases PPM1A and PPM1B to dephosphorylate their physiological substrates in cells.
Chida T, Ando M, Matsuki T, Masu Y, Nagaura Y, Takano-Yamamoto T, Tamura S, Kobayashi T
The Biochemical journal449(3)741-9(2013 Feb)

Ppm1E is an in cellulo AMP-activated protein kinase phosphatase.
Voss M, Paterson J, Kelsall IR, Mart?n-Granados C, Hastie CJ, Peggie MW, Cohen PT
Cellular signalling23(1)114-24(2011 Jan)

Negative regulation of multifunctional Ca2+/calmodulin-dependent protein kinases: physiological and pharmacological significance of protein phosphatases.
Ishida A, Sueyoshi N, Shigeri Y, Kameshita I
British journal of pharmacology154(4)729-40(2008 Jun)

Post-translational excision of the carboxyl-terminal segment of CaM kinase phosphatase N and its cytosolic occurrence in the brain.
Kitani T, Okuno S, Nakamura Y, Tokuno H, Takeuchi M, Fujisawa H
Journal of neurochemistry96(2)374-84(2006 Jan)

Identification of major Ca(2+)/calmodulin-dependent protein kinase phosphatase-binding proteins in brain: biochemical analysis of the interaction.
Ishida A, Tada Y, Nimura T, Sueyoshi N, Katoh T, Takeuchi M, Fujisawa H, Taniguchi T, Kameshita I
Archives of biochemistry and biophysics435(1)134-46(2005 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CaM kinase phosphatase N is involved in the regulation of not only CaM kinase IV but also CaM kinases II and I.
Title: Post-translational excision of the carboxyl-terminal segment of CaM kinase phosphatase N and its cytosolic occurrence in the brain.

The results suggest that CaMKP regulates the phosphorylation level of GAPDH in the CaMKP-GAPDH complex by dephosphorylating and deactivating CaMKs that are responsible for the phosphorylation of GAPDH.
Title: Identification of major Ca(2+)/calmodulin-dependent protein kinase phosphatase-binding proteins in brain: biochemical analysis of the interaction.

CaM kinase phosphatase N occurs inside the cellular nucleus of PC-12 cells [Ppm1e].
Title: Subcellular distributions of rat CaM kinase phosphatase N and other members of the CaM kinase regulatory system.

The following Ppm1e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppm1e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05095	NM_198773.1 Latest version!	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1E (Ppm1e), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppm1e ( NM_198773.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_942068.1 Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1E (Ppm1e), mRNA.

Price & Availability↑

CloneID	ORa05095
Clone ID Related Accession (Same CDS sequence)	NM_198773.1
Accession Version	NM_198773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	protein phosphatase 1E
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB081729.1. On Dec 17, 2003 this sequence version replaced XM_340873.1. ##Evidence-Data-START## Transcript exon combination :: AB081729.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGGCT GCATCCCTGA GGAGAAAACT TACCGGCGCT TCCTGGAGCT GTTCCTGGGC 
GAGTTCCGCG GACCGTGCGG CGGCGGCGAG CCAGAGCCCG AACCCGAATC CGAGCCCGAG 
CCCGAGCCCG AAGCGGAGCT GGTCGCGGCT GAGGCGGCCG AGGCTTCGGG GGAGGACCCG 
GGGGAGGACG CTGCGACGGT AGAGGCGGCC GAGGAGGGGG TGCAGGACCA AGACCCGGAG 
CCCGAAGAGG AGGCGGTGGA AGAGGAGGCG GCGGCCGAGG GCGAGGAGGA GGAGGAAGAG 
GAGGAAGCGG CTGCCCCGGG GCACTCGGCC GTGCCGCCGC CGCAGCCCCA GCTGCCGCCG 
CTGCCCCCGC TCCCGCGGCC GCTGTCGGAG CGCATCACCC GAGAGGAGGT GGAGGGCGAG 
AGCCTGGACC TGTGCCTGCA GCAGCTCTAC AAATATAACT GCCCTTCCTT TCTGGCTGCC 
GCTTTAGCCA GAGCCACATC AGATGAAGTC CTCCAGAGTG ACCTTTCTGC TCACTGTATC 
CCAAAGGAGA CGGACGGCAC AGAAGGGACT GTGGAAATTG AGACAGTGAA ATTAGCCCGT 
TCTGTCTTCA GCAAACTACA CGAGATTTGC TGCAATTGGG TGAAGGACTT CCCTCTACGC 
AGGAGGCCAC AGATTTATTA TGAGACGTCA ATCCATGCCA TCAAGAACAT GCGAAGGAAA 
ATGGAGGACA AACATGTCTG CATCCCTGAC TTTAACATGC TCTTCAACCT AGAGGACCAG 
GAGGAACAAG CATACTTTGC AGTGTTTGAT GGCCATGGGG GAGTGGATGC TGCTATATAT 
GCCTCGGTTC ACCTACACGT TAACTTAGTG CGCCAGGAGA TGTTTCCCCA TGACCCTGCT 
GAGGCTCTCT GCCGGGCCTT CCGTGTCACA GATGAGCGGT TTGTGCAGAA GGCAGCCAGG 
GAGAGTTTAC GATGTGGGAC CACAGGAGTA GTGACTTTTA TCAGAGGCAA CATGCTCCAT 
GTGGCATGGG TGGGCGACTC CCAGGTCATG CTGGTAAGAA AGGGCCAAGC TGTTGAACTC 
ATGAAGCCTC ACAAGCCAGA CAGAGAGGAT GAAAAGCAGC GAATTGAGGC CCTTGGAGGT 
TGTGTGGTTT GGTTTGGTGC CTGGAGGGTG AATGGAAGCC TGTCCGTCTC CAGAGCCATC 
GGAGATGCTG AACACAAGCC ATATATCTGT GGAGATGCAG ATTCTGCCTC CACTGTTCTG 
GATGGGACTG AAGACTACCT CATTCTGGCC TGTGATGGCT TCTATGATAC CGTGAACCCT 
GATGAGGCAG TGAAGGTTGT GTCTGACCAC TTGAAAGAGA ATAATGGAGA CAGCAGCATG 
GTTGCCCACA AATTAGTAGC ATCAGCTCGT GATGCGGGGT CAAGTGATAA CATCACTGTT 
ATTGTGGTAT TCCTGAGGGA CATGAACAAA GCTGTAAATG TTAGTGAGGA ATCAGATTGG 
ACAGAGAACT CTTTTCAAGG AGGGCAAGAA GATGGTGGGG ATGATAAGGA GAATCATGGA 
GAGTGCAAAC GCCCTTGGCC ACAGCACCAG TGCTCAGCAC CAGCCGATCT AGGCTATGAG 
GGGCGTGTGG ATTCATTCAC TGATAGAACT AGCCTGAGCC CAGGGCCCCA AATCAACGTG 
CTGGAAGATC CAGACTACTT AGATCTCACA CAAATAGAAA CAAGCAAACC TCACAGTACC 
CAATTTTTGC CACCAGTTGA GATGATTGGT CCTGGTGCAC CAAAGAAAGC ATATGTTAAT 
GAGCTAATAA TGGAGGAGAG CTCAGTCACG CCATCACAGC CTGAACGGAG TGGTGCTGGA 
GAGCTTCTGG TTTCTTTTAA TTTGGGTTCA ACAGGGCAAC AGATATGCAG AATGGAGAAC 
TTGTCCCCTG TCTATTCTGG GTTGGAAAAT GAACAGTTCA AATCCCCGGG GAAAAGAGCG 
TCTAGATTGT ATCATTTACG CCACCACTAC TCCAAAAGGC AGCGTGGATT CAGGTTTAAT 
CCAAAGTTTT ATTCATTTTT TTCTGCTCAA GAGTCTTCTC GAAAAATAGG CATTAGCCTC 
TCCTCACTTA CTCGAAGTGG GAAGAGAAAC AAGATGTTAA GAAGTTCTTT GCCATGGAGG 
GAAAATAGTT GGGAAGGATA TAGTGGAAAC ATGGCGATCA GAAAGCGTAA TAATATTTCA 
TGCCCAGACC TTCCCTGGGA CTATAAAATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_942068.1
CDS	121..2373
Translation	MAGCIPEEKTYRRFLELFLGEFRGPCGGGEPEPEPESEPEPEPEAELVAAEAAEASGEDPGEDAATVEAAEEGVQDQDPEPEEEAVEEEAAAEGEEEEEEEEAAAPGHSAVPPPQPQLPPLPPLPRPLSERITREEVEGESLDLCLQQLYKYNCPSFLAAALARATSDEVLQSDLSAHCIPKETDGTEGTVEIETVKLARSVFSKLHEICCNWVKDFPLRRRPQIYYETSIHAIKNMRRKMEDKHVCIPDFNMLFNLEDQEEQAYFAVFDGHGGVDAAIYASVHLHVNLVRQEMFPHDPAEALCRAFRVTDERFVQKAARESLRCGTTGVVTFIRGNMLHVAWVGDSQVMLVRKGQAVELMKPHKPDREDEKQRIEALGGCVVWFGAWRVNGSLSVSRAIGDAEHKPYICGDADSASTVLDGTEDYLILACDGFYDTVNPDEAVKVVSDHLKENNGDSSMVAHKLVASARDAGSSDNITVIVVFLRDMNKAVNVSEESDWTENSFQGGQEDGGDDKENHGECKRPWPQHQCSAPADLGYEGRVDSFTDRTSLSPGPQINVLEDPDYLDLTQIETSKPHSTQFLPPVEMIGPGAPKKAYVNELIMEESSVTPSQPERSGAGELLVSFNLGSTGQQICRMENLSPVYSGLENEQFKSPGKRASRLYHLRHHYSKRQRGFRFNPKFYSFFSAQESSRKIGISLSSLTRSGKRNKMLRSSLPWRENSWEGYSGNMAIRKRNNISCPDLPWDYKI

Target ORF information:

RefSeq Version	NM_198773.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1E (Ppm1e), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198773.1

ORF Insert Sequence:

ATGGCGGGCT GCATCCCTGA GGAGAAAACT TACCGGCGCT TCCTGGAGCT GTTCCTGGGC 
GAGTTCCGCG GACCGTGCGG CGGCGGCGAG CCAGAGCCCG AACCCGAATC CGAGCCCGAG 
CCCGAGCCCG AAGCGGAGCT GGTCGCGGCT GAGGCGGCCG AGGCTTCGGG GGAGGACCCG 
GGGGAGGACG CTGCGACGGT AGAGGCGGCC GAGGAGGGGG TGCAGGACCA AGACCCGGAG 
CCCGAAGAGG AGGCGGTGGA AGAGGAGGCG GCGGCCGAGG GCGAGGAGGA GGAGGAAGAG 
GAGGAAGCGG CTGCCCCGGG GCACTCGGCC GTGCCGCCGC CGCAGCCCCA GCTGCCGCCG 
CTGCCCCCGC TCCCGCGGCC GCTGTCGGAG CGCATCACCC GAGAGGAGGT GGAGGGCGAG 
AGCCTGGACC TGTGCCTGCA GCAGCTCTAC AAATATAACT GCCCTTCCTT TCTGGCTGCC 
GCTTTAGCCA GAGCCACATC AGATGAAGTC CTCCAGAGTG ACCTTTCTGC TCACTGTATC 
CCAAAGGAGA CGGACGGCAC AGAAGGGACT GTGGAAATTG AGACAGTGAA ATTAGCCCGT 
TCTGTCTTCA GCAAACTACA CGAGATTTGC TGCAATTGGG TGAAGGACTT CCCTCTACGC 
AGGAGGCCAC AGATTTATTA TGAGACGTCA ATCCATGCCA TCAAGAACAT GCGAAGGAAA 
ATGGAGGACA AACATGTCTG CATCCCTGAC TTTAACATGC TCTTCAACCT AGAGGACCAG 
GAGGAACAAG CATACTTTGC AGTGTTTGAT GGCCATGGGG GAGTGGATGC TGCTATATAT 
GCCTCGGTTC ACCTACACGT TAACTTAGTG CGCCAGGAGA TGTTTCCCCA TGACCCTGCT 
GAGGCTCTCT GCCGGGCCTT CCGTGTCACA GATGAGCGGT TTGTGCAGAA GGCAGCCAGG 
GAGAGTTTAC GATGTGGGAC CACAGGAGTA GTGACTTTTA TCAGAGGCAA CATGCTCCAT 
GTGGCATGGG TGGGCGACTC CCAGGTCATG CTGGTAAGAA AGGGCCAAGC TGTTGAACTC 
ATGAAGCCTC ACAAGCCAGA CAGAGAGGAT GAAAAGCAGC GAATTGAGGC CCTTGGAGGT 
TGTGTGGTTT GGTTTGGTGC CTGGAGGGTG AATGGAAGCC TGTCCGTCTC CAGAGCCATC 
GGAGATGCTG AACACAAGCC ATATATCTGT GGAGATGCAG ATTCTGCCTC CACTGTTCTG 
GATGGGACTG AAGACTACCT CATTCTGGCC TGTGATGGCT TCTATGATAC CGTGAACCCT 
GATGAGGCAG TGAAGGTTGT GTCTGACCAC TTGAAAGAGA ATAATGGAGA CAGCAGCATG 
GTTGCCCACA AATTAGTAGC ATCAGCTCGT GATGCGGGGT CAAGTGATAA CATCACTGTT 
ATTGTGGTAT TCCTGAGGGA CATGAACAAA GCTGTAAATG TTAGTGAGGA ATCAGATTGG 
ACAGAGAACT CTTTTCAAGG AGGGCAAGAA GATGGTGGGG ATGATAAGGA GAATCATGGA 
GAGTGCAAAC GCCCTTGGCC ACAGCACCAG TGCTCAGCAC CAGCCGATCT AGGCTATGAG 
GGGCGTGTGG ATTCATTCAC TGATAGAACT AGCCTGAGCC CAGGGCCCCA AATCAACGTG 
CTGGAAGATC CAGACTACTT AGATCTCACA CAAATAGAAA CAAGCAAACC TCACAGTACC 
CAATTTTTGC CACCAGTTGA GATGATTGGT CCTGGTGCAC CAAAGAAAGC ATATGTTAAT 
GAGCTAATAA TGGAGGAGAG CTCAGTCACG CCATCACAGC CTGAACGGAG TGGTGCTGGA 
GAGCTTCTGG TTTCTTTTAA TTTGGGTTCA ACAGGGCAAC AGATATGCAG AATGGAGAAC 
TTGTCCCCTG TCTATTCTGG GTTGGAAAAT GAACAGTTCA AATCCCCGGG GAAAAGAGCG 
TCTAGATTGT ATCATTTACG CCACCACTAC TCCAAAAGGC AGCGTGGATT CAGGTTTAAT 
CCAAAGTTTT ATTCATTTTT TTCTGCTCAA GAGTCTTCTC GAAAAATAGG CATTAGCCTC 
TCCTCACTTA CTCGAAGTGG GAAGAGAAAC AAGATGTTAA GAAGTTCTTT GCCATGGAGG 
GAAAATAGTT GGGAAGGATA TAGTGGAAAC ATGGCGATCA GAAAGCGTAA TAATATTTCA 
TGCCCAGACC TTCCCTGGGA CTATAAAATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

N-Myristoylation is essential for protein phosphatases PPM1A and PPM1B to dephosphorylate their physiological substrates in cells.
The Biochemical journal449(3)741-9(2013 Feb)
Chida T,Ando M,Matsuki T,Masu Y,Nagaura Y,Takano-Yamamoto T,Tamura S,Kobayashi T

Ppm1E is an in cellulo AMP-activated protein kinase phosphatase.
Cellular signalling23(1)114-24(2011 Jan)
Voss M,Paterson J,Kelsall IR,Mart?n-Granados C,Hastie CJ,Peggie MW,Cohen PT

Negative regulation of multifunctional Ca2+/calmodulin-dependent protein kinases: physiological and pharmacological significance of protein phosphatases.
British journal of pharmacology154(4)729-40(2008 Jun)
Ishida A,Sueyoshi N,Shigeri Y,Kameshita I

Post-translational excision of the carboxyl-terminal segment of CaM kinase phosphatase N and its cytosolic occurrence in the brain.
Journal of neurochemistry96(2)374-84(2006 Jan)
Kitani T,Okuno S,Nakamura Y,Tokuno H,Takeuchi M,Fujisawa H

Identification of major Ca(2+)/calmodulin-dependent protein kinase phosphatase-binding proteins in brain: biochemical analysis of the interaction.
Archives of biochemistry and biophysics435(1)134-46(2005 Mar)
Ishida A,Tada Y,Nimura T,Sueyoshi N,Katoh T,Takeuchi M,Fujisawa H,Taniguchi T,Kameshita I