Ppm1e cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppm1e
Entrez Gene ID	320472
Full Name	protein phosphatase 1E (PP2C domain containing)
Synonyms	AW049266,B930008A12Rik,POPX1,PP2CH,mKIAA1072
General protein information	Preferred Names protein phosphatase 1E (PP2C domain containing) Names protein phosphatase 1E ca(2+)/calmodulin-dependent protein kinase phosphatase N caMKN caMKP-N caMKP-nucleus partner of PIX 1 partner of PIX-alpha partner of PIXA
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 C

mRNA and Protein(s)

mRNA	Protein	Name
NM_177167.4	NP_796141.2	protein phosphatase 1E

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ppm1e	NP_942068.1
Canis lupus familiaris (dog)	PPM1E	XP_853253.1
Gallus gallus (chicken)	PPM1E	XP_415871.4
Homo sapiens (human)	PPM1E	NP_055721.3
Danio rerio (zebrafish)	ppm1e	NP_001018354.1
Pan troglodytes (chimpanzee)	PPM1E	XP_523813.4
	LOC711012	XP_002800484.1
Bos taurus (cattle)	PPM1E	XP_002695648.3
Mus musculus (house mouse)	Ppm1e	NP_796141.2
Xenopus tropicalis (tropical clawed frog)	ppm1e	XP_002934392.1

Gene Ontology
Bibliography

Related articles in PubMed

N-Myristoylation is essential for protein phosphatases PPM1A and PPM1B to dephosphorylate their physiological substrates in cells.
Chida T, Ando M, Matsuki T, Masu Y, Nagaura Y, Takano-Yamamoto T, Tamura S, Kobayashi T
The Biochemical journal449(3)741-9(2013 Feb)

Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Aizawa H, Yuasa S, Nakajima D, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes10(1)35-48(2003 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ppm1e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppm1e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu06012	NM_177167.4 Latest version!	Mus musculus protein phosphatase 1E (PP2C domain containing) (Ppm1e), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$440.30 $629.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppm1e ( NM_177167.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_796141.2 Mus musculus protein phosphatase 1E (PP2C domain containing) (Ppm1e), mRNA.

Price & Availability↑

CloneID	OMu06012
Clone ID Related Accession (Same CDS sequence)	NM_177167.4
Accession Version	NM_177167.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	protein phosphatase 1E
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL596130.13. On Nov 17, 2006 this sequence version replaced NM_177167.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK122434.1, AK036583.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGGCT GCATCCCTGA GGAGAAAACT TACCGGCGCT TCCTGGAGCT GTTCCTGGGC 
GAGTTCCGCG GACCGTGCGG TGGCGGCGAG CCAGAGCCCG AACCCGAATC CGAGCCCGAG 
CCCGAGCCCG AAGCCGAGCT AGTCGCGGCT GAGGCGGCCG AGGCTTCGGG GGAGGAACCG 
GGGGAGGACG CTGCGACGGT AGAGGCGACC GAGGAGGGGG AGCAGGACCA AGACCCCGAG 
CCCGAAGACG AGGCGGTGGA GGAGGAGACG GCGACGGAGG GCGAGGAGGA GGAAGAGGAG 
GAAGCGGCTG CCCCGGGGCA CTCGGCCGTG CCGCCACCGC CGCAGCCCCA GCTGCCACCG 
CTGCCCCCGC TCCCACGGCC GCTGTCGGAG CGCATCACCC GAGAGGAGGT GGAGGGCGAG 
AGCCTGGACC TGTGCCTGCA GCAGCTCTAC AAATATAACT GCCCTTCCTT TCTGGCTGCT 
GCTTTAGCCA GAGCCACATC AGATGAAGTC CTCCAGAGTG ACCTTTCTGC TCACTGTATC 
CCAAAGGAAA CGGACGGCAC AGAAGGGACT GTGGAAATTG AGACAGTGAA ATTGGCCCGT 
TCTGTCTTCA GCAAACTACA TGAGATCTGC TGCAGTTGGG TGAAGGACTT CCCTCTGCGC 
AGGAGGCCTC AGATTTATTA TGAGACGTCA ATTCATGCCA TCAAGAACAT GCGAAGGAAA 
ATGGAGGACA AACACGTCTG CATTCCTGAC TTTAACATGC TCTTCAACCT AGAGGACCAG 
GAGGAACAAG CATACTTTGC AGTGTTTGAT GGCCATGGGG GAGTGGACGC TGCTATTTAT 
GCCTCCGTTC ACCTACACGT TAACTTAGTG CGCCAGGAGA TGTTTCCCCA TGATCCTGCT 
GAGGCTCTCT GCCGGGCCTT CCGTGTCACA GATGAGCGGT TTGTGCAGAA GGCAGCCAGG 
GAGAGCTTAC GGTGTGGGAC CACAGGAGTA GTGACTTTTA TCAGAGGCAA CATGCTCCAC 
GTGGCATGGG TGGGCGATTC CCAGGTCATG CTGGTAAGAA AGGGGCAAGC TGTTGAACTA 
ATGAAACCAC ACAAGCCAGA CAGAGAGGAT GAAAAGCAGC GAATTGAGGC CCTTGGAGGC 
TGTGTGGTTT GGTTTGGCGC CTGGAGGGTG AATGGAAGCC TGTCCGTCTC CAGAGCCATC 
GGAGATGCTG AGCACAAGCC GTATATCTGT GGAGACGCAG ACTCGGCCTC CACTGTTTTG 
GATGGGACTG AAGACTACCT CATTCTGGCC TGTGACGGCT TCTATGACAC CGTGAACCCT 
GACGAGGCAG TGAAGGTTGT GTCTGACCAC TTGAAAGAGA ATAACGGAGA CAGCAGCATG 
GTTGCCCACA AATTAGTAGC GTCGGCTCGT GATGCGGGGT CAAGTGATAA CATCACTGTT 
ATTGTGGTAT TCCTGAGGGA CATGAACAAG GCTGTAAATG TTAGTGAGGA GTCAGAGTGG 
ACAGAGAACT CTTTTCAAGG AGGGCAAGAA GATGGTGGGG ATGATAAGGA GACTCATGGA 
GAGTGCAAGC GCCCTTGGCC ACAGCACCAG TGCTCAGCAC CCGCCGATCT AGGCTATGAG 
GGGCGAGTGG ATTCATTCAC TGATAGAACT AGCCTCAGCC CAGGGCCCCA AATCAACGTG 
CTGGAAGATC CAGACTACCT AGATCTCACA CAAATAGAAG CAAGCAAACC TCACAGTACC 
CAATTTTTGC CACCAGTTGA GATGATTGGT CCTGGTGCAC CAAAGAAAGA TCTTAATGAG 
CTAATAATGG AGGAGAGATC AGTCAAGTCA TCATTGCCTG AACGGAGTGG TGCTGGAGAG 
CCTCGGGTTT CTTTTAATTT GGGTTCAACA GGGCAACAGA TATGCAGAAT GGAGAACTTG 
TCTCCTGTCA GTTCTGGGTT GGAAAATGAA CAGTTCAAAT CCCGGGGGAA GACAGCGTCT 
AGGTTGTATC ATTTACGCCA CCACTACTCC AAAAGGCAGC GTGGATTCAG GTTTAATCCA 
AAGTTTTACT CGTTTCTGTC TGCTCGAGAG CCTTCTCACA AAATAGGCAT TAGCCTCTCC 
TCACTTACTC GAAGTGGGAA GAGAAACAAG ATGTTAAGAA GTTCTTTGCC ATGGAGGGAA 
AATAGTTGGG AAGGATATAG TGGAAATGTG AAGATCAGAA AGCGTAATGA TATTCCATGC 
CCAGACTTTC CCTGGAGCTA TAAAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_796141.2
CDS	92..2341
Translation	MAGCIPEEKTYRRFLELFLGEFRGPCGGGEPEPEPESEPEPEPEAELVAAEAAEASGEEPGEDAATVEATEEGEQDQDPEPEDEAVEEETATEGEEEEEEEAAAPGHSAVPPPPQPQLPPLPPLPRPLSERITREEVEGESLDLCLQQLYKYNCPSFLAAALARATSDEVLQSDLSAHCIPKETDGTEGTVEIETVKLARSVFSKLHEICCSWVKDFPLRRRPQIYYETSIHAIKNMRRKMEDKHVCIPDFNMLFNLEDQEEQAYFAVFDGHGGVDAAIYASVHLHVNLVRQEMFPHDPAEALCRAFRVTDERFVQKAARESLRCGTTGVVTFIRGNMLHVAWVGDSQVMLVRKGQAVELMKPHKPDREDEKQRIEALGGCVVWFGAWRVNGSLSVSRAIGDAEHKPYICGDADSASTVLDGTEDYLILACDGFYDTVNPDEAVKVVSDHLKENNGDSSMVAHKLVASARDAGSSDNITVIVVFLRDMNKAVNVSEESEWTENSFQGGQEDGGDDKETHGECKRPWPQHQCSAPADLGYEGRVDSFTDRTSLSPGPQINVLEDPDYLDLTQIEASKPHSTQFLPPVEMIGPGAPKKDLNELIMEERSVKSSLPERSGAGEPRVSFNLGSTGQQICRMENLSPVSSGLENEQFKSRGKTASRLYHLRHHYSKRQRGFRFNPKFYSFLSAREPSHKIGISLSSLTRSGKRNKMLRSSLPWRENSWEGYSGNVKIRKRNDIPCPDFPWSYKI

Target ORF information:

RefSeq Version	NM_177167.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 1E (PP2C domain containing) (Ppm1e), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177167.4

ORF Insert Sequence:

ATGGCGGGCT GCATCCCTGA GGAGAAAACT TACCGGCGCT TCCTGGAGCT GTTCCTGGGC 
GAGTTCCGCG GACCGTGCGG TGGCGGCGAG CCAGAGCCCG AACCCGAATC CGAGCCCGAG 
CCCGAGCCCG AAGCCGAGCT AGTCGCGGCT GAGGCGGCCG AGGCTTCGGG GGAGGAACCG 
GGGGAGGACG CTGCGACGGT AGAGGCGACC GAGGAGGGGG AGCAGGACCA AGACCCCGAG 
CCCGAAGACG AGGCGGTGGA GGAGGAGACG GCGACGGAGG GCGAGGAGGA GGAAGAGGAG 
GAAGCGGCTG CCCCGGGGCA CTCGGCCGTG CCGCCACCGC CGCAGCCCCA GCTGCCACCG 
CTGCCCCCGC TCCCACGGCC GCTGTCGGAG CGCATCACCC GAGAGGAGGT GGAGGGCGAG 
AGCCTGGACC TGTGCCTGCA GCAGCTCTAC AAATATAACT GCCCTTCCTT TCTGGCTGCT 
GCTTTAGCCA GAGCCACATC AGATGAAGTC CTCCAGAGTG ACCTTTCTGC TCACTGTATC 
CCAAAGGAAA CGGACGGCAC AGAAGGGACT GTGGAAATTG AGACAGTGAA ATTGGCCCGT 
TCTGTCTTCA GCAAACTACA TGAGATCTGC TGCAGTTGGG TGAAGGACTT CCCTCTGCGC 
AGGAGGCCTC AGATTTATTA TGAGACGTCA ATTCATGCCA TCAAGAACAT GCGAAGGAAA 
ATGGAGGACA AACACGTCTG CATTCCTGAC TTTAACATGC TCTTCAACCT AGAGGACCAG 
GAGGAACAAG CATACTTTGC AGTGTTTGAT GGCCATGGGG GAGTGGACGC TGCTATTTAT 
GCCTCCGTTC ACCTACACGT TAACTTAGTG CGCCAGGAGA TGTTTCCCCA TGATCCTGCT 
GAGGCTCTCT GCCGGGCCTT CCGTGTCACA GATGAGCGGT TTGTGCAGAA GGCAGCCAGG 
GAGAGCTTAC GGTGTGGGAC CACAGGAGTA GTGACTTTTA TCAGAGGCAA CATGCTCCAC 
GTGGCATGGG TGGGCGATTC CCAGGTCATG CTGGTAAGAA AGGGGCAAGC TGTTGAACTA 
ATGAAACCAC ACAAGCCAGA CAGAGAGGAT GAAAAGCAGC GAATTGAGGC CCTTGGAGGC 
TGTGTGGTTT GGTTTGGCGC CTGGAGGGTG AATGGAAGCC TGTCCGTCTC CAGAGCCATC 
GGAGATGCTG AGCACAAGCC GTATATCTGT GGAGACGCAG ACTCGGCCTC CACTGTTTTG 
GATGGGACTG AAGACTACCT CATTCTGGCC TGTGACGGCT TCTATGACAC CGTGAACCCT 
GACGAGGCAG TGAAGGTTGT GTCTGACCAC TTGAAAGAGA ATAACGGAGA CAGCAGCATG 
GTTGCCCACA AATTAGTAGC GTCGGCTCGT GATGCGGGGT CAAGTGATAA CATCACTGTT 
ATTGTGGTAT TCCTGAGGGA CATGAACAAG GCTGTAAATG TTAGTGAGGA GTCAGAGTGG 
ACAGAGAACT CTTTTCAAGG AGGGCAAGAA GATGGTGGGG ATGATAAGGA GACTCATGGA 
GAGTGCAAGC GCCCTTGGCC ACAGCACCAG TGCTCAGCAC CCGCCGATCT AGGCTATGAG 
GGGCGAGTGG ATTCATTCAC TGATAGAACT AGCCTCAGCC CAGGGCCCCA AATCAACGTG 
CTGGAAGATC CAGACTACCT AGATCTCACA CAAATAGAAG CAAGCAAACC TCACAGTACC 
CAATTTTTGC CACCAGTTGA GATGATTGGT CCTGGTGCAC CAAAGAAAGA TCTTAATGAG 
CTAATAATGG AGGAGAGATC AGTCAAGTCA TCATTGCCTG AACGGAGTGG TGCTGGAGAG 
CCTCGGGTTT CTTTTAATTT GGGTTCAACA GGGCAACAGA TATGCAGAAT GGAGAACTTG 
TCTCCTGTCA GTTCTGGGTT GGAAAATGAA CAGTTCAAAT CCCGGGGGAA GACAGCGTCT 
AGGTTGTATC ATTTACGCCA CCACTACTCC AAAAGGCAGC GTGGATTCAG GTTTAATCCA 
AAGTTTTACT CGTTTCTGTC TGCTCGAGAG CCTTCTCACA AAATAGGCAT TAGCCTCTCC 
TCACTTACTC GAAGTGGGAA GAGAAACAAG ATGTTAAGAA GTTCTTTGCC ATGGAGGGAA 
AATAGTTGGG AAGGATATAG TGGAAATGTG AAGATCAGAA AGCGTAATGA TATTCCATGC 
CCAGACTTTC CCTGGAGCTA TAAAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

N-Myristoylation is essential for protein phosphatases PPM1A and PPM1B to dephosphorylate their physiological substrates in cells.
The Biochemical journal449(3)741-9(2013 Feb)
Chida T,Ando M,Matsuki T,Masu Y,Nagaura Y,Takano-Yamamoto T,Tamura S,Kobayashi T

Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes10(1)35-48(2003 Feb)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Aizawa H,Yuasa S,Nakajima D,Nagase T,Ohara O,Koga H

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Ppm1e cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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