Mdga1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mdga1
Entrez Gene ID	309659
Full Name	MAM domain containing glycosylphosphatidylinositol anchor 1
General protein information	Preferred Names MAM domain containing glycosylphosphatidylinositol anchor 1 Names MAM domain-containing glycosylphosphatidylinositol anchor protein 1 ig6M
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20p12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001107618.1	NP_001101088.1	MAM domain-containing glycosylphosphatidylinositol anchor protein 1 precursor
XM_006256207.3	XP_006256269.1	MAM domain-containing glycosylphosphatidylinositol anchor protein 1 isoform X1
XM_017601653.1	XP_017457142.1	MAM domain-containing glycosylphosphatidylinositol anchor protein 1 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mdga1	NP_001074629.1
Canis lupus familiaris (dog)	MDGA1	XP_532128.3
Danio rerio (zebrafish)	LOC569180	XP_697640.6
Homo sapiens (human)	MDGA1	NP_705691.1
Bos taurus (cattle)	MDGA1	NP_001179811.1
Rattus norvegicus (Norway rat)	Mdga1	NP_001101088.1
Gallus gallus (chicken)	MDGA1	NP_001038112.1
Pan troglodytes (chimpanzee)	MDGA1	XP_527378.2
	MDGA1	XP_001117048.2

Gene Ontology
Bibliography

Related articles in PubMed

Molecular Mechanism of MDGA1: Regulation of Neuroligin 2:Neurexin Trans-synaptic Bridges.
Gangwar SP, Zhong X, Seshadrinathan S, Chen H, Machius M, Rudenko G
Neuron94(6)1132-1141.e4(2017 Jun)

Interaction between autism-linked MDGAs and neuroligins suppresses inhibitory synapse development.
Pettem KL, Yokomaku D, Takahashi H, Ge Y, Craig AM
The Journal of cell biology200(3)321-36(2013 Feb)

MDGAs interact selectively with neuroligin-2 but not other neuroligins to regulate inhibitory synapse development.
Lee K, Kim Y, Lee SJ, Qiang Y, Lee D, Lee HW, Kim H, Je HS, S?dhof TC, Ko J
Proceedings of the National Academy of Sciences of the United States of America110(1)336-41(2013 Jan)

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
B?hm D, Keller K, Pieter J, Boehm N, Wolters D, Siggelkow W, Lebrecht A, Schmidt M, K?lbl H, Pfeiffer N, Grus FH
Oncology reports28(2)429-38(2012 Aug)

IgSF molecule MDGA1 is involved in radial migration and positioning of a subset of cortical upper-layer neurons.
Ishikawa T, Gotoh N, Murayama C, Abe T, Iwashita M, Matsuzaki F, Suzuki T, Yamamoto T
Developmental dynamics : an official publication of the American Association of Anatomists240(1)96-107(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MDGA binding and suppression of synaptogenic activity was selective for neuroligin-2 and not neuroligin-1 excitatory synapse organizer.
Title: Interaction between autism-linked MDGAs and neuroligins suppresses inhibitory synapse development.

MDGAs interact selectively with neuroligin-2 but not other neuroligins to regulate inhibitory synapse development.
Title: MDGAs interact selectively with neuroligin-2 but not other neuroligins to regulate inhibitory synapse development.

The following Mdga1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mdga1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa11408	NM_001107618.1 Latest version!	Rattus norvegicus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$552.30 $789.00
ORa39303	XM_006256207.3 Latest version!	Rattus norvegicus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa60103	XM_017601653.1 Latest version!	Rattus norvegicus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mdga1 ( NM_001107618.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101088.1 Rattus norvegicus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), mRNA.

Price & Availability↑

CloneID	ORa11408
Clone ID Related Accession (Same CDS sequence)	NM_001107618.1
Accession Version	NM_001107618.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Rattus norvegicus(Norway rat)
Product	MAM domain-containing glycosylphosphatidylinositol anchor protein 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473988.1. On or before Oct 4, 2007 this sequence version replaced XM_241623.4, XM_001070102.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGTGA CCTGCCTTCT ACTTCTGGCG CTGATCCCCT TCCACTGTCG GGGACAAGGA 
GTCTACGCTC CAGCCCAGGC CCAGATCGTG CATGCAGGGC AGGCCTGTGT GGTGAAGGAG 
GATAATATCA GCGAGCGGGT CTATACCATC CGGGAGAGCG ACACCCTGGT GCTGCAGTGC 
CTGGTTACTG GGCACCCTCG GCCCCAGGTG CGGTGGACCA AAACTGCGGG CAGCGCATCC 
GACAAGTTCC AGGAGACTTC GGTGTTCAAT GAAACTCTGC GGATCGAGCG CATCGCCCGC 
ACGCAGGGAG GCCGCTACTA CTGCAAGGCG GAGAACGGAG TGGGCGTGCC GGCCATCAAG 
TCCATCCGCG TGGACGTGCA GTACTTGGAT GAACCTGTGT TGACGGTGCA TCAGACGGTG 
AGCGACGTCC GAGGCAACTT CTACCAGGAG AAGACCGTGT TTCTACGCTG TACCGTCAGT 
TCCAACCCGC CCGCCCGCTT TATCTGGAAG CGGGGCTCCG ACACCCTGTC CCACAGCCAG 
GACAATGGGG TTGACATCTA CGAGCCTCTC TATACTCAGG GGGAGACGAA GGTCTTGAAG 
CTGAAGAACC TGCGGCCCCA AGACTATGCC AGCTACACCT GCCAGGTGTC TGTGCGTAAC 
GTGTGCGGCA TCCCTGACAA GTCTATCACC TTCCAGCTCA CCAACACCAC GGCACCTCCA 
ACTTTGAAGC TGTCTGTGAA CGAAACTCTG GTGGTGAACC CTGGGGAGAA TGTGACAGTG 
CAGTGCCTGC TGACGGGCGG CGATCCCCTC CCACAGCTGC ACTGGTCCCA CGGACCTGGC 
CCACTGCCTC TGGGTGCGCT GGCTCAGGGT GGTACCCTTA GCATTCCTTC AGTGCAGGCC 
CGGGATTCCG GTTACTACAA CTGCACAGCC ACCAACAATG TAGGCAACCC AGCCAAGAAG 
ACGGTCAACC TGCTGGTGCG ATCCTTGAAG AATGCCACCT TCCAGATCAC CCCTGACATG 
ATCAAAGAGA GCGAGAACAT TCAACTTGGT CAGGATCTGA AGCTCTCATG CCATGTGGAC 
GCGGTGCCCC AGGAGAAGGT GAACTACCAG TGGTTTAAAA ACGGCAAGCC GGCACGCACG 
TCCAAGCGGC TGCTGGTGAC CCGAAATGAC CCTGAGCTGC CCGCTGTCAC CAGCAGCCTG 
GAGCTCATTG ACCTGCACTT CAGCGACTAC GGCACTTATC TGTGCGTGGC TTCCTTCCCG 
GGGTCACCTG TGCCTGACCT CAGCGTAGAG GTCAACATCT CCTCCGAGAC AGTACCGCCC 
ACCATCAGTG TGCCCAAGGG CCGAGCAGTG GTGACAGTTC GGGAGGGCTC TCCTGCTGAG 
CTTCAGTGCG AAGTGCGCGG CAAACCCCGG CCTCCAGTGC TGTGGTCCCG CGTGGACAAG 
GAGGCCGCCC TTCTGCCCTC GGGGCTGGCT CTGGAAGAGA CTCCGGATGG GAAGCTCCGA 
GTGGAGCGCG TGAGCCGCGA AATGAGCGGC ACCTATCGCT GCCAGACGGC TCGCTATAAT 
GGCTTCAACG TACGAGCCCG GGAGGCTCAG GTGCAGCTGA CAGTGCACTT CCCTCCAGAG 
GTGGAGCCCA GTTCCCAAGA CGTGCGCCAG GCGCTGGGCC GCCCTGTGCT CCTGCGCTGC 
TCGCTCCTGC GTGGCAGTCC CCAGCGCATC GCCTCGGCCG TGTGGCGCTT CAAAGGACAG 
CTGCTGCCCC CGCCGCCTGT TCTCCCCGCC GCTGCCGCCG AGGGCCCGGA CCACGCCGAG 
CTGCGCCTGG ACGCCCTGAC CCGTGACAGC AGCGGTAACT ACGAATGCAG CGTCTCCAAC 
GACGTGGGCT CAGCCACCTG CCTCTTCCAG GTCTCCGCCA AAGCCTACAG CCCGGAGTTT 
TACTTCGACA CCCCCAACCC CACCCGCAGC CACAAGCTGT CCAAGAACTA CTCCTATGTG 
CTACAGTGGA CGCAGAGGGA GCCGGACGCC GTGGACCCTG TGCTCAACTA CAGACTCAGC 
ATCCGCCAGT TGAACCAGCA CAATGCCATG GTCAAGGCCA TCCCTGTTCG GCGTGTGGAG 
AAGGGGCAGC TGCTGGAGTA CACCTTGACT GACCTCCGTG TGCCCCACAG CTATGAGATC 
CACCTCACAC CCTACACCAC CTTTGGGGCT GGAGACATGG CTTCCCGAGT CATCCACTAC 
ACAGAGCCTA TCAACTCGCC CAGCCTGTCA GACAATACCT GTCACTTTGA GGATGAGAAA 
ATCTGCGGAT ACACCCAGGA TCTGACAGAC AACTTCGACT GGACACGGCA GAATGCGCTT 
ACCCAGAATC CCAAGCGCTC TCCCAATACT GGGCCACCTA CGGACATCAG TGGCACCCCC 
GAAGGCTACT ACATGTTCAT TGAGACATCA AGACCCCGGG AGCTGGGGGA CCGGGCAAGG 
CTGGTGAGTC CCCTGTACAA CGCCAGCGCC AAGTTCTACT GCTCCCTCAA CCTTCTGGTG 
AGGTCCAGGA ACAAAGGCAC TCTAGACACG CATGCCTGGT CCCTCAGCGG TAATAAAGGT 
AACGTGTGGC AACAGGCCCA CGTGCCCATC AACCCCAGCG GACCCTTCCA GATTATTTTT 
GAGGGGGTTC GAGGCTCGGG CTACCTGGGG GATATTGCCA TAGATGATGT CACACTGAAG 
AAGGGAGAGT GTCCCCGGAG GCAGATGGAT CCCAACAAAG TGGTGGTGAT GCCAGGCAGT 
GGAGCTCCCC GCCTGTCCAG CCTGCAGCTT TGGGGATCCA TGACCATCTT CCTCTTGGCG 
TTGCAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101088.1
CDS	360..3191
Translation	MEVTCLLLLALIPFHCRGQGVYAPAQAQIVHAGQACVVKEDNISERVYTIRESDTLVLQCLVTGHPRPQVRWTKTAGSASDKFQETSVFNETLRIERIARTQGGRYYCKAENGVGVPAIKSIRVDVQYLDEPVLTVHQTVSDVRGNFYQEKTVFLRCTVSSNPPARFIWKRGSDTLSHSQDNGVDIYEPLYTQGETKVLKLKNLRPQDYASYTCQVSVRNVCGIPDKSITFQLTNTTAPPTLKLSVNETLVVNPGENVTVQCLLTGGDPLPQLHWSHGPGPLPLGALAQGGTLSIPSVQARDSGYYNCTATNNVGNPAKKTVNLLVRSLKNATFQITPDMIKESENIQLGQDLKLSCHVDAVPQEKVNYQWFKNGKPARTSKRLLVTRNDPELPAVTSSLELIDLHFSDYGTYLCVASFPGSPVPDLSVEVNISSETVPPTISVPKGRAVVTVREGSPAELQCEVRGKPRPPVLWSRVDKEAALLPSGLALEETPDGKLRVERVSREMSGTYRCQTARYNGFNVRAREAQVQLTVHFPPEVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVLPAAAAEGPDHAELRLDALTRDSSGNYECSVSNDVGSATCLFQVSAKAYSPEFYFDTPNPTRSHKLSKNYSYVLQWTQREPDAVDPVLNYRLSIRQLNQHNAMVKAIPVRRVEKGQLLEYTLTDLRVPHSYEIHLTPYTTFGAGDMASRVIHYTEPINSPSLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPEGYYMFIETSRPRELGDRARLVSPLYNASAKFYCSLNLLVRSRNKGTLDTHAWSLSGNKGNVWQQAHVPINPSGPFQIIFEGVRGSGYLGDIAIDDVTLKKGECPRRQMDPNKVVVMPGSGAPRLSSLQLWGSMTIFLLALQR

Target ORF information:

RefSeq Version	NM_001107618.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107618.1

ORF Insert Sequence:

ATGGAGGTGA CCTGCCTTCT ACTTCTGGCG CTGATCCCCT TCCACTGTCG GGGACAAGGA 
GTCTACGCTC CAGCCCAGGC CCAGATCGTG CATGCAGGGC AGGCCTGTGT GGTGAAGGAG 
GATAATATCA GCGAGCGGGT CTATACCATC CGGGAGAGCG ACACCCTGGT GCTGCAGTGC 
CTGGTTACTG GGCACCCTCG GCCCCAGGTG CGGTGGACCA AAACTGCGGG CAGCGCATCC 
GACAAGTTCC AGGAGACTTC GGTGTTCAAT GAAACTCTGC GGATCGAGCG CATCGCCCGC 
ACGCAGGGAG GCCGCTACTA CTGCAAGGCG GAGAACGGAG TGGGCGTGCC GGCCATCAAG 
TCCATCCGCG TGGACGTGCA GTACTTGGAT GAACCTGTGT TGACGGTGCA TCAGACGGTG 
AGCGACGTCC GAGGCAACTT CTACCAGGAG AAGACCGTGT TTCTACGCTG TACCGTCAGT 
TCCAACCCGC CCGCCCGCTT TATCTGGAAG CGGGGCTCCG ACACCCTGTC CCACAGCCAG 
GACAATGGGG TTGACATCTA CGAGCCTCTC TATACTCAGG GGGAGACGAA GGTCTTGAAG 
CTGAAGAACC TGCGGCCCCA AGACTATGCC AGCTACACCT GCCAGGTGTC TGTGCGTAAC 
GTGTGCGGCA TCCCTGACAA GTCTATCACC TTCCAGCTCA CCAACACCAC GGCACCTCCA 
ACTTTGAAGC TGTCTGTGAA CGAAACTCTG GTGGTGAACC CTGGGGAGAA TGTGACAGTG 
CAGTGCCTGC TGACGGGCGG CGATCCCCTC CCACAGCTGC ACTGGTCCCA CGGACCTGGC 
CCACTGCCTC TGGGTGCGCT GGCTCAGGGT GGTACCCTTA GCATTCCTTC AGTGCAGGCC 
CGGGATTCCG GTTACTACAA CTGCACAGCC ACCAACAATG TAGGCAACCC AGCCAAGAAG 
ACGGTCAACC TGCTGGTGCG ATCCTTGAAG AATGCCACCT TCCAGATCAC CCCTGACATG 
ATCAAAGAGA GCGAGAACAT TCAACTTGGT CAGGATCTGA AGCTCTCATG CCATGTGGAC 
GCGGTGCCCC AGGAGAAGGT GAACTACCAG TGGTTTAAAA ACGGCAAGCC GGCACGCACG 
TCCAAGCGGC TGCTGGTGAC CCGAAATGAC CCTGAGCTGC CCGCTGTCAC CAGCAGCCTG 
GAGCTCATTG ACCTGCACTT CAGCGACTAC GGCACTTATC TGTGCGTGGC TTCCTTCCCG 
GGGTCACCTG TGCCTGACCT CAGCGTAGAG GTCAACATCT CCTCCGAGAC AGTACCGCCC 
ACCATCAGTG TGCCCAAGGG CCGAGCAGTG GTGACAGTTC GGGAGGGCTC TCCTGCTGAG 
CTTCAGTGCG AAGTGCGCGG CAAACCCCGG CCTCCAGTGC TGTGGTCCCG CGTGGACAAG 
GAGGCCGCCC TTCTGCCCTC GGGGCTGGCT CTGGAAGAGA CTCCGGATGG GAAGCTCCGA 
GTGGAGCGCG TGAGCCGCGA AATGAGCGGC ACCTATCGCT GCCAGACGGC TCGCTATAAT 
GGCTTCAACG TACGAGCCCG GGAGGCTCAG GTGCAGCTGA CAGTGCACTT CCCTCCAGAG 
GTGGAGCCCA GTTCCCAAGA CGTGCGCCAG GCGCTGGGCC GCCCTGTGCT CCTGCGCTGC 
TCGCTCCTGC GTGGCAGTCC CCAGCGCATC GCCTCGGCCG TGTGGCGCTT CAAAGGACAG 
CTGCTGCCCC CGCCGCCTGT TCTCCCCGCC GCTGCCGCCG AGGGCCCGGA CCACGCCGAG 
CTGCGCCTGG ACGCCCTGAC CCGTGACAGC AGCGGTAACT ACGAATGCAG CGTCTCCAAC 
GACGTGGGCT CAGCCACCTG CCTCTTCCAG GTCTCCGCCA AAGCCTACAG CCCGGAGTTT 
TACTTCGACA CCCCCAACCC CACCCGCAGC CACAAGCTGT CCAAGAACTA CTCCTATGTG 
CTACAGTGGA CGCAGAGGGA GCCGGACGCC GTGGACCCTG TGCTCAACTA CAGACTCAGC 
ATCCGCCAGT TGAACCAGCA CAATGCCATG GTCAAGGCCA TCCCTGTTCG GCGTGTGGAG 
AAGGGGCAGC TGCTGGAGTA CACCTTGACT GACCTCCGTG TGCCCCACAG CTATGAGATC 
CACCTCACAC CCTACACCAC CTTTGGGGCT GGAGACATGG CTTCCCGAGT CATCCACTAC 
ACAGAGCCTA TCAACTCGCC CAGCCTGTCA GACAATACCT GTCACTTTGA GGATGAGAAA 
ATCTGCGGAT ACACCCAGGA TCTGACAGAC AACTTCGACT GGACACGGCA GAATGCGCTT 
ACCCAGAATC CCAAGCGCTC TCCCAATACT GGGCCACCTA CGGACATCAG TGGCACCCCC 
GAAGGCTACT ACATGTTCAT TGAGACATCA AGACCCCGGG AGCTGGGGGA CCGGGCAAGG 
CTGGTGAGTC CCCTGTACAA CGCCAGCGCC AAGTTCTACT GCTCCCTCAA CCTTCTGGTG 
AGGTCCAGGA ACAAAGGCAC TCTAGACACG CATGCCTGGT CCCTCAGCGG TAATAAAGGT 
AACGTGTGGC AACAGGCCCA CGTGCCCATC AACCCCAGCG GACCCTTCCA GATTATTTTT 
GAGGGGGTTC GAGGCTCGGG CTACCTGGGG GATATTGCCA TAGATGATGT CACACTGAAG 
AAGGGAGAGT GTCCCCGGAG GCAGATGGAT CCCAACAAAG TGGTGGTGAT GCCAGGCAGT 
GGAGCTCCCC GCCTGTCCAG CCTGCAGCTT TGGGGATCCA TGACCATCTT CCTCTTGGCG 
TTGCAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdga1 ( XM_006256207.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256269.1 Rattus norvegicus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa39303
Clone ID Related Accession (Same CDS sequence)	XM_006256207.3
Accession Version	XM_006256207.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	MAM domain-containing glycosylphosphatidylinositol anchor protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256207.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGA CCTGCCTTCT ACTTCTGGCG CTGATCCCCT TCCACTGTCG GGGACAAGGA 
GTCTACGCTC CAGCCCAGGC CCAGATCGTG CATGCAGGGC AGGCCTGTGT GGTGAAGGAG 
GATAATATCA GCGAGCGGGT CTATACCATC CGGGAGAGCG ACACCCTGGT GCTGCAGTGC 
CTGGTTACTG GGCACCCTCG GCCCCAGGTG CGGTGGACCA AAACTGCGGG CAGCGCATCC 
GACAAGTTCC AGGAGACTTC GGTGTTCAAT GAAACTCTGC GGATCGAGCG CATCGCCCGC 
ACGCAGGGAG GCCGCTACTA CTGCAAGGCG GAGAACGGAG TGGGCGTGCC GGCCATCAAG 
TCCATCCGCG TGGACGTGCA GTACTTGGAT GAACCTGTGT TGACGGTGCA TCAGACGGTG 
AGCGACGTCC GAGGCAACTT CTACCAGGAG AAGACCGTGT TTCTACGCTG TACCGTCAGT 
TCCAACCCGC CCGCCCGCTT TATCTGGAAG CGGGGCTCCG ACACCCTGTC CCACAGCCAG 
GACAATGGGG TTGACATCTA CGAGCCTCTC TATACTCAGG GGGAGACGAA GGTCTTGAAG 
CTGAAGAACC TGCGGCCCCA AGACTATGCC AGCTACACCT GCCAGGTGTC TGTGCGTAAC 
GTGTGCGGCA TCCCTGACAA GTCTATCACC TTCCAGCTCA CCAACACCAC GGCACCTCCA 
ACTTTGAAGC TGTCTGTGAA CGAAACTCTG GTGGTGAACC CTGGGGAGAA TGTGACAGTG 
CAGTGCCTGC TGACGGGCGG CGATCCCCTC CCACAGCTGC ACTGGTCCCA CGGACCTGGC 
CCACTGCCTC TGGGTGCGCT GGCTCAGGGT GGTACCCTTA GCATTCCTTC AGTGCAGGCC 
CGGGATTCCG GTTACTACAA CTGCACAGCC ACCAACAATG TAGGCAACCC AGCCAAGAAG 
ACGGTCAACC TGCTGGTGCG ATCCTTGAAG AATGCCACCT TCCAGATCAC CCCTGACATG 
ATCAAAGAGA GCGAGAACAT TCAACTTGGT CAGGATCTGA AGCTCTCATG CCATGTGGAC 
GCGGTGCCCC AGGAGAAGGT GAACTACCAG TGGTTTAAAA ACGGCAAGCC GGCACGCACG 
TCCAAGCGGC TGCTGGTGAC CCGAAATGAC CCTGAGCTGC CCGCTGTCAC CAGCAGCCTG 
GAGCTCATTG ACCTGCACTT CAGCGACTAC GGCACTTATC TGTGCGTGGC TTCCTTCCCG 
GGGTCACCTG TGCCTGACCT CAGCGTAGAG GTCAACATCT CCTCCGAGAC AGTACCGCCC 
ACCATCAGTG TGCCCAAGGG CCGAGCAGTG GTGACAGTTC GGGAGGGCTC TCCTGCTGAG 
CTTCAGTGCG AAGTGCGCGG CAAACCCCGG CCTCCAGTGC TGTGGTCCCG CGTGGACAAG 
GAGGCCGCCC TTCTGCCCTC GGGGCTGGCT CTGGAAGAGA CTCCGGATGG GAAGCTCCGA 
GTGGAGCGCG TGAGCCGCGA AATGAGCGGC ACCTATCGCT GCCAGACGGC TCGCTATAAT 
GGCTTCAACG TACGAGCCCG GGAGGCTCAG GTGCAGCTGA CAGTGCACTT CCCTCCAGAG 
GTGGAGCCCA GTTCCCAAGA CGTGCGCCAG GCGCTGGGCC GCCCTGTGCT CCTGCGCTGC 
TCGCTCCTGC GTGGCAGTCC CCAGCGCATC GCCTCGGCCG TGTGGCGCTT CAAAGGACAG 
CTGCTGCCCC CGCCGCCTGT TCTCCCCGCC GCTGCCGCCG AGGGCCCGGA CCACGCCGAG 
CTGCGCCTGG ACGCCCTGAC CCGTGACAGC AGCGGTAACT ACGAATGCAG CGTCTCCAAC 
GACGTGGGCT CAGCCACCTG CCTCTTCCAG GTCTCCGCCA AAGCCTACAG CCCGGAGTTT 
TACTTCGACA CCCCCAACCC CACCCGCAGC CACAAGCTGT CCAAGAACTA CTCCTATGTG 
CTACAGTGGA CGCAGAGGGA GCCGGACGCC GTGGACCCTG TGCTCAACTA CAGACTCAGC 
ATCCGCCAGT TGAACCAGCA CAATGCCATG GTCAAGGCCA TCCCTGTTCG GCGTGTGGAG 
AAGGGGCAGC TGCTGGAGTA CACCTTGACT GACCTCCGTG TGCCCCACAG CTATGAGATC 
CACCTCACAC CCTACACCAC CTTTGGGGCT GGAGACATGG CTTCCCGAGT CATCCACTAC 
ACAGAGCCTA TCAACTCGCC CAGCCTGTCA GACAATACCT GTCACTTTGA GGATGAGAAA 
ATCTGCGGAT ACACCCAGGA TCTGACAGAC AACTTCGACT GGACACGGCA GAATGCGCTT 
ACCCAGAATC CCAAGCGCTC TCCCAATACT GGGCCACCTA CGGACATCAG TGGCACCCCC 
GAAGGCTACT ACATGTTCAT TGAGACATCA AGACCCCGGG AGCTGGGGGA CCGGGCAAGG 
CTGGTGAGTC CCCTGTACAA CGCCAGCGCC AAGTTCTACT GCGTCTCTTT CTTCTACCAC 
ATGTACGGCA AACACATCGG CTCCCTCAAC CTTCTGGTGA GGTCCAGGAA CAAAGGCACT 
CTAGACACGC ATGCCTGGTC CCTCAGCGGT AATAAAGGTA ACGTGTGGCA ACAGGCCCAC 
GTGCCCATCA ACCCCAGCGG ACCCTTCCAG ATTATTTTTG AGGGGGTTCG AGGCTCGGGC 
TACCTGGGGG ATATTGCCAT AGATGATGTC ACACTGAAGA AGGGAGAGTG TCCCCGGAGG 
CAGATGGATC CCAACAAAGT GGTGGTGATG CCAGGCAGTG GAGCCCCCCG CCTGTCCAGC 
CTGCAGCTTT GGGGATCCAT GACCATCTTC CTCTTGGCGT TGCAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256269.1
CDS	908..3778
Translation	MEVTCLLLLALIPFHCRGQGVYAPAQAQIVHAGQACVVKEDNISERVYTIRESDTLVLQCLVTGHPRPQVRWTKTAGSASDKFQETSVFNETLRIERIARTQGGRYYCKAENGVGVPAIKSIRVDVQYLDEPVLTVHQTVSDVRGNFYQEKTVFLRCTVSSNPPARFIWKRGSDTLSHSQDNGVDIYEPLYTQGETKVLKLKNLRPQDYASYTCQVSVRNVCGIPDKSITFQLTNTTAPPTLKLSVNETLVVNPGENVTVQCLLTGGDPLPQLHWSHGPGPLPLGALAQGGTLSIPSVQARDSGYYNCTATNNVGNPAKKTVNLLVRSLKNATFQITPDMIKESENIQLGQDLKLSCHVDAVPQEKVNYQWFKNGKPARTSKRLLVTRNDPELPAVTSSLELIDLHFSDYGTYLCVASFPGSPVPDLSVEVNISSETVPPTISVPKGRAVVTVREGSPAELQCEVRGKPRPPVLWSRVDKEAALLPSGLALEETPDGKLRVERVSREMSGTYRCQTARYNGFNVRAREAQVQLTVHFPPEVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVLPAAAAEGPDHAELRLDALTRDSSGNYECSVSNDVGSATCLFQVSAKAYSPEFYFDTPNPTRSHKLSKNYSYVLQWTQREPDAVDPVLNYRLSIRQLNQHNAMVKAIPVRRVEKGQLLEYTLTDLRVPHSYEIHLTPYTTFGAGDMASRVIHYTEPINSPSLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPEGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGTLDTHAWSLSGNKGNVWQQAHVPINPSGPFQIIFEGVRGSGYLGDIAIDDVTLKKGECPRRQMDPNKVVVMPGSGAPRLSSLQLWGSMTIFLLALQR

Target ORF information:

RefSeq Version	XM_006256207.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256207.3

ORF Insert Sequence:

ATGGAGGTGA CCTGCCTTCT ACTTCTGGCG CTGATCCCCT TCCACTGTCG GGGACAAGGA 
GTCTACGCTC CAGCCCAGGC CCAGATCGTG CATGCAGGGC AGGCCTGTGT GGTGAAGGAG 
GATAATATCA GCGAGCGGGT CTATACCATC CGGGAGAGCG ACACCCTGGT GCTGCAGTGC 
CTGGTTACTG GGCACCCTCG GCCCCAGGTG CGGTGGACCA AAACTGCGGG CAGCGCATCC 
GACAAGTTCC AGGAGACTTC GGTGTTCAAT GAAACTCTGC GGATCGAGCG CATCGCCCGC 
ACGCAGGGAG GCCGCTACTA CTGCAAGGCG GAGAACGGAG TGGGCGTGCC GGCCATCAAG 
TCCATCCGCG TGGACGTGCA GTACTTGGAT GAACCTGTGT TGACGGTGCA TCAGACGGTG 
AGCGACGTCC GAGGCAACTT CTACCAGGAG AAGACCGTGT TTCTACGCTG TACCGTCAGT 
TCCAACCCGC CCGCCCGCTT TATCTGGAAG CGGGGCTCCG ACACCCTGTC CCACAGCCAG 
GACAATGGGG TTGACATCTA CGAGCCTCTC TATACTCAGG GGGAGACGAA GGTCTTGAAG 
CTGAAGAACC TGCGGCCCCA AGACTATGCC AGCTACACCT GCCAGGTGTC TGTGCGTAAC 
GTGTGCGGCA TCCCTGACAA GTCTATCACC TTCCAGCTCA CCAACACCAC GGCACCTCCA 
ACTTTGAAGC TGTCTGTGAA CGAAACTCTG GTGGTGAACC CTGGGGAGAA TGTGACAGTG 
CAGTGCCTGC TGACGGGCGG CGATCCCCTC CCACAGCTGC ACTGGTCCCA CGGACCTGGC 
CCACTGCCTC TGGGTGCGCT GGCTCAGGGT GGTACCCTTA GCATTCCTTC AGTGCAGGCC 
CGGGATTCCG GTTACTACAA CTGCACAGCC ACCAACAATG TAGGCAACCC AGCCAAGAAG 
ACGGTCAACC TGCTGGTGCG ATCCTTGAAG AATGCCACCT TCCAGATCAC CCCTGACATG 
ATCAAAGAGA GCGAGAACAT TCAACTTGGT CAGGATCTGA AGCTCTCATG CCATGTGGAC 
GCGGTGCCCC AGGAGAAGGT GAACTACCAG TGGTTTAAAA ACGGCAAGCC GGCACGCACG 
TCCAAGCGGC TGCTGGTGAC CCGAAATGAC CCTGAGCTGC CCGCTGTCAC CAGCAGCCTG 
GAGCTCATTG ACCTGCACTT CAGCGACTAC GGCACTTATC TGTGCGTGGC TTCCTTCCCG 
GGGTCACCTG TGCCTGACCT CAGCGTAGAG GTCAACATCT CCTCCGAGAC AGTACCGCCC 
ACCATCAGTG TGCCCAAGGG CCGAGCAGTG GTGACAGTTC GGGAGGGCTC TCCTGCTGAG 
CTTCAGTGCG AAGTGCGCGG CAAACCCCGG CCTCCAGTGC TGTGGTCCCG CGTGGACAAG 
GAGGCCGCCC TTCTGCCCTC GGGGCTGGCT CTGGAAGAGA CTCCGGATGG GAAGCTCCGA 
GTGGAGCGCG TGAGCCGCGA AATGAGCGGC ACCTATCGCT GCCAGACGGC TCGCTATAAT 
GGCTTCAACG TACGAGCCCG GGAGGCTCAG GTGCAGCTGA CAGTGCACTT CCCTCCAGAG 
GTGGAGCCCA GTTCCCAAGA CGTGCGCCAG GCGCTGGGCC GCCCTGTGCT CCTGCGCTGC 
TCGCTCCTGC GTGGCAGTCC CCAGCGCATC GCCTCGGCCG TGTGGCGCTT CAAAGGACAG 
CTGCTGCCCC CGCCGCCTGT TCTCCCCGCC GCTGCCGCCG AGGGCCCGGA CCACGCCGAG 
CTGCGCCTGG ACGCCCTGAC CCGTGACAGC AGCGGTAACT ACGAATGCAG CGTCTCCAAC 
GACGTGGGCT CAGCCACCTG CCTCTTCCAG GTCTCCGCCA AAGCCTACAG CCCGGAGTTT 
TACTTCGACA CCCCCAACCC CACCCGCAGC CACAAGCTGT CCAAGAACTA CTCCTATGTG 
CTACAGTGGA CGCAGAGGGA GCCGGACGCC GTGGACCCTG TGCTCAACTA CAGACTCAGC 
ATCCGCCAGT TGAACCAGCA CAATGCCATG GTCAAGGCCA TCCCTGTTCG GCGTGTGGAG 
AAGGGGCAGC TGCTGGAGTA CACCTTGACT GACCTCCGTG TGCCCCACAG CTATGAGATC 
CACCTCACAC CCTACACCAC CTTTGGGGCT GGAGACATGG CTTCCCGAGT CATCCACTAC 
ACAGAGCCTA TCAACTCGCC CAGCCTGTCA GACAATACCT GTCACTTTGA GGATGAGAAA 
ATCTGCGGAT ACACCCAGGA TCTGACAGAC AACTTCGACT GGACACGGCA GAATGCGCTT 
ACCCAGAATC CCAAGCGCTC TCCCAATACT GGGCCACCTA CGGACATCAG TGGCACCCCC 
GAAGGCTACT ACATGTTCAT TGAGACATCA AGACCCCGGG AGCTGGGGGA CCGGGCAAGG 
CTGGTGAGTC CCCTGTACAA CGCCAGCGCC AAGTTCTACT GCGTCTCTTT CTTCTACCAC 
ATGTACGGCA AACACATCGG CTCCCTCAAC CTTCTGGTGA GGTCCAGGAA CAAAGGCACT 
CTAGACACGC ATGCCTGGTC CCTCAGCGGT AATAAAGGTA ACGTGTGGCA ACAGGCCCAC 
GTGCCCATCA ACCCCAGCGG ACCCTTCCAG ATTATTTTTG AGGGGGTTCG AGGCTCGGGC 
TACCTGGGGG ATATTGCCAT AGATGATGTC ACACTGAAGA AGGGAGAGTG TCCCCGGAGG 
CAGATGGATC CCAACAAAGT GGTGGTGATG CCAGGCAGTG GAGCCCCCCG CCTGTCCAGC 
CTGCAGCTTT GGGGATCCAT GACCATCTTC CTCTTGGCGT TGCAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdga1 ( XM_017601653.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017457142.1 Rattus norvegicus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), transcript variant X2, mRNA.

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CloneID	ORa60103
Clone ID Related Accession (Same CDS sequence)	XM_017601653.1
Accession Version	XM_017601653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	MAM domain-containing glycosylphosphatidylinositol anchor protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGA CCTGCCTTCT ACTTCTGGCG CTGATCCCCT TCCACTGTCG GGGACAAGGA 
GTCTACGCTC CAGCCCAGGC CCAGATCGTG CATGCAGGGC AGGCCTGTGT GGTGAAGGAG 
GATAATATCA GCGAGCGGGT CTATACCATC CGGGAGAGCG ACACCCTGGT GCTGCAGTGC 
CTGGTTACTG GGCACCCTCG GCCCCAGGTG CGGTGGACCA AAACTGCGGG CAGCGCATCC 
GACAAGTTCC AGGAGACTTC GGTGTTCAAT GAAACTCTGC GGATCGAGCG CATCGCCCGC 
ACGCAGGGAG GCCGCTACTA CTGCAAGGCG GAGAACGGAG TGGGCGTGCC GGCCATCAAG 
TCCATCCGCG TGGACGTGCA GTACTTGGAT GAACCTGTGT TGACGGTGCA TCAGACGGTG 
AGCGACGTCC GAGGCAACTT CTACCAGGAG AAGACCGTGT TTCTACGCTG TACCGTCAGT 
TCCAACCCGC CCGCCCGCTT TATCTGGAAG CGGGGCTCCG ACACCCTGTC CCACAGCCAG 
GACAATGGGG TTGACATCTA CGAGCCTCTC TATACTCAGG GGGAGACGAA GGTCTTGAAG 
CTGAAGAACC TGCGGCCCCA AGACTATGCC AGCTACACCT GCCAGGTGTC TGTGCGTAAC 
GTGTGCGGCA TCCCTGACAA GTCTATCACC TTCCAGCTCA CCAACACCAC GGCACCTCCA 
ACTTTGAAGC TGTCTGTGAA CGAAACTCTG GTGGTGAACC CTGGGGAGAA TGTGACAGTG 
CAGTGCCTGC TGACGGGCGG CGATCCCCTC CCACAGCTGC ACTGGTCCCA CGGACCTGGC 
CCACTGCCTC TGGGTGCGCT GGCTCAGGGT GGTACCCTTA GCATTCCTTC AGTGCAGGCC 
CGGGATTCCG GTTACTACAA CTGCACAGCC ACCAACAATG TAGGCAACCC AGCCAAGAAG 
ACGGTCAACC TGCTGGTGCG ATCCTTGAAG AATGCCACCT TCCAGATCAC CCCTGACATG 
ATCAAAGAGA GCGAGAACAT TCAACTTGGT CAGGATCTGA AGCTCTCATG CCATGTGGAC 
GCGGTGCCCC AGGAGAAGGT GAACTACCAG TGGTTTAAAA ACGGCAAGCC GGCACGCACG 
TCCAAGCGGC TGCTGGTGAC CCGAAATGAC CCTGAGCTGC CCGCTGTCAC CAGCAGCCTG 
GAGCTCATTG ACCTGCACTT CAGCGACTAC GGCACTTATC TGTGCGTGGC TTCCTTCCCG 
GGGTCACCTG TGCCTGACCT CAGCGTAGAG GTCAACATCT CCTCCGAGAC AGTACCGCCC 
ACCATCAGTG TGCCCAAGGG CCGAGCAGTG GTGACAGTTC GGGAGGGCTC TCCTGCTGAG 
CTTCAGTGCG AAGTGCGCGG CAAACCCCGG CCTCCAGTGC TGTGGTCCCG CGTGGACAAG 
GAGGCCGCCC TTCTGCCCTC GGGGCTGGCT CTGGAAGAGA CTCCGGATGG GAAGCTCCGA 
GTGGAGCGCG TGAGCCGCGA AATGAGCGGC ACCTATCGCT GCCAGACGGC TCGCTATAAT 
GGCTTCAACG TACGAGCCCG GGAGGCTCAG GTGCAGCTGA CAGTGCACTT CCCTCCAGAG 
GTGGAGCCCA GTTCCCAAGA CGTGCGCCAG GCGCTGGGCC GCCCTGTGCT CCTGCGCTGC 
TCGCTCCTGC GTGGCAGTCC CCAGCGCATC GCCTCGGCCG TGTGGCGCTT CAAAGGACAG 
CTGCTGCCCC CGCCGCCTGT TCTCCCCGCC GCTGCCGCCG AGGGCCCGGA CCACGCCGAG 
CTGCGCCTGG ACGCCCTGAC CCGTGACAGC AGCGGTAACT ACGAATGCAG CGTCTCCAAC 
GACGTGGGCT CAGCCACCTG CCTCTTCCAG GTCTCCGCTG GGGACCGAAC CCAGGGCCTT 
ACGCTTGCTA GCCACAAGCT GTCCAAGAAC TACTCCTATG TGCTACAGTG GACGCAGAGG 
GAGCCGGACG CCGTGGACCC TGTGCTCAAC TACAGACTCA GCATCCGCCA GTTGAACCAG 
CACAATGCCA TGGTCAAGGC CATCCCTGTT CGGCGTGTGG AGAAGGGGCA GCTGCTGGAG 
TACACCTTGA CTGACCTCCG TGTGCCCCAC AGCTATGAGA TCCACCTCAC ACCCTACACC 
ACCTTTGGGG CTGGAGACAT GGCTTCCCGA GTCATCCACT ACACAGAGCC TATCAACTCG 
CCCAGCCTGT CAGACAATAC CTGTCACTTT GAGGATGAGA AAATCTGCGG ATACACCCAG 
GATCTGACAG ACAACTTCGA CTGGACACGG CAGAATGCGC TTACCCAGAA TCCCAAGCGC 
TCTCCCAATA CTGGGCCACC TACGGACATC AGTGGCACCC CCGAAGGCTA CTACATGTTC 
ATTGAGACAT CAAGACCCCG GGAGCTGGGG GACCGGGCAA GGCTGGTGAG TCCCCTGTAC 
AACGCCAGCG CCAAGTTCTA CTGCGTCTCT TTCTTCTACC ACATGTACGG CAAACACATC 
GGCTCCCTCA ACCTTCTGGT GAGGTCCAGG AACAAAGGCA CTCTAGACAC GCATGCCTGG 
TCCCTCAGCG GTAATAAAGG TAACGTGTGG CAACAGGCCC ACGTGCCCAT CAACCCCAGC 
GGACCCTTCC AGATTATTTT TGAGGGGGTT CGAGGCTCGG GCTACCTGGG GGATATTGCC 
ATAGATGATG TCACACTGAA GAAGGGAGAG TGTCCCCGGA GGCAGATGGA TCCCAACAAA 
GTGGTGGTGA TGCCAGGCAG TGGAGCCCCC CGCCTGTCCA GCCTGCAGCT TTGGGGATCC 
ATGACCATCT TCCTCTTGGC GTTGCAGAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017457142.1
CDS	910..3762
Translation	MEVTCLLLLALIPFHCRGQGVYAPAQAQIVHAGQACVVKEDNISERVYTIRESDTLVLQCLVTGHPRPQVRWTKTAGSASDKFQETSVFNETLRIERIARTQGGRYYCKAENGVGVPAIKSIRVDVQYLDEPVLTVHQTVSDVRGNFYQEKTVFLRCTVSSNPPARFIWKRGSDTLSHSQDNGVDIYEPLYTQGETKVLKLKNLRPQDYASYTCQVSVRNVCGIPDKSITFQLTNTTAPPTLKLSVNETLVVNPGENVTVQCLLTGGDPLPQLHWSHGPGPLPLGALAQGGTLSIPSVQARDSGYYNCTATNNVGNPAKKTVNLLVRSLKNATFQITPDMIKESENIQLGQDLKLSCHVDAVPQEKVNYQWFKNGKPARTSKRLLVTRNDPELPAVTSSLELIDLHFSDYGTYLCVASFPGSPVPDLSVEVNISSETVPPTISVPKGRAVVTVREGSPAELQCEVRGKPRPPVLWSRVDKEAALLPSGLALEETPDGKLRVERVSREMSGTYRCQTARYNGFNVRAREAQVQLTVHFPPEVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVLPAAAAEGPDHAELRLDALTRDSSGNYECSVSNDVGSATCLFQVSAGDRTQGLTLASHKLSKNYSYVLQWTQREPDAVDPVLNYRLSIRQLNQHNAMVKAIPVRRVEKGQLLEYTLTDLRVPHSYEIHLTPYTTFGAGDMASRVIHYTEPINSPSLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPEGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGTLDTHAWSLSGNKGNVWQQAHVPINPSGPFQIIFEGVRGSGYLGDIAIDDVTLKKGECPRRQMDPNKVVVMPGSGAPRLSSLQLWGSMTIFLLALQR

Target ORF information:

RefSeq Version	XM_017601653.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601653.1

ORF Insert Sequence:

ATGGAGGTGA CCTGCCTTCT ACTTCTGGCG CTGATCCCCT TCCACTGTCG GGGACAAGGA 
GTCTACGCTC CAGCCCAGGC CCAGATCGTG CATGCAGGGC AGGCCTGTGT GGTGAAGGAG 
GATAATATCA GCGAGCGGGT CTATACCATC CGGGAGAGCG ACACCCTGGT GCTGCAGTGC 
CTGGTTACTG GGCACCCTCG GCCCCAGGTG CGGTGGACCA AAACTGCGGG CAGCGCATCC 
GACAAGTTCC AGGAGACTTC GGTGTTCAAT GAAACTCTGC GGATCGAGCG CATCGCCCGC 
ACGCAGGGAG GCCGCTACTA CTGCAAGGCG GAGAACGGAG TGGGCGTGCC GGCCATCAAG 
TCCATCCGCG TGGACGTGCA GTACTTGGAT GAACCTGTGT TGACGGTGCA TCAGACGGTG 
AGCGACGTCC GAGGCAACTT CTACCAGGAG AAGACCGTGT TTCTACGCTG TACCGTCAGT 
TCCAACCCGC CCGCCCGCTT TATCTGGAAG CGGGGCTCCG ACACCCTGTC CCACAGCCAG 
GACAATGGGG TTGACATCTA CGAGCCTCTC TATACTCAGG GGGAGACGAA GGTCTTGAAG 
CTGAAGAACC TGCGGCCCCA AGACTATGCC AGCTACACCT GCCAGGTGTC TGTGCGTAAC 
GTGTGCGGCA TCCCTGACAA GTCTATCACC TTCCAGCTCA CCAACACCAC GGCACCTCCA 
ACTTTGAAGC TGTCTGTGAA CGAAACTCTG GTGGTGAACC CTGGGGAGAA TGTGACAGTG 
CAGTGCCTGC TGACGGGCGG CGATCCCCTC CCACAGCTGC ACTGGTCCCA CGGACCTGGC 
CCACTGCCTC TGGGTGCGCT GGCTCAGGGT GGTACCCTTA GCATTCCTTC AGTGCAGGCC 
CGGGATTCCG GTTACTACAA CTGCACAGCC ACCAACAATG TAGGCAACCC AGCCAAGAAG 
ACGGTCAACC TGCTGGTGCG ATCCTTGAAG AATGCCACCT TCCAGATCAC CCCTGACATG 
ATCAAAGAGA GCGAGAACAT TCAACTTGGT CAGGATCTGA AGCTCTCATG CCATGTGGAC 
GCGGTGCCCC AGGAGAAGGT GAACTACCAG TGGTTTAAAA ACGGCAAGCC GGCACGCACG 
TCCAAGCGGC TGCTGGTGAC CCGAAATGAC CCTGAGCTGC CCGCTGTCAC CAGCAGCCTG 
GAGCTCATTG ACCTGCACTT CAGCGACTAC GGCACTTATC TGTGCGTGGC TTCCTTCCCG 
GGGTCACCTG TGCCTGACCT CAGCGTAGAG GTCAACATCT CCTCCGAGAC AGTACCGCCC 
ACCATCAGTG TGCCCAAGGG CCGAGCAGTG GTGACAGTTC GGGAGGGCTC TCCTGCTGAG 
CTTCAGTGCG AAGTGCGCGG CAAACCCCGG CCTCCAGTGC TGTGGTCCCG CGTGGACAAG 
GAGGCCGCCC TTCTGCCCTC GGGGCTGGCT CTGGAAGAGA CTCCGGATGG GAAGCTCCGA 
GTGGAGCGCG TGAGCCGCGA AATGAGCGGC ACCTATCGCT GCCAGACGGC TCGCTATAAT 
GGCTTCAACG TACGAGCCCG GGAGGCTCAG GTGCAGCTGA CAGTGCACTT CCCTCCAGAG 
GTGGAGCCCA GTTCCCAAGA CGTGCGCCAG GCGCTGGGCC GCCCTGTGCT CCTGCGCTGC 
TCGCTCCTGC GTGGCAGTCC CCAGCGCATC GCCTCGGCCG TGTGGCGCTT CAAAGGACAG 
CTGCTGCCCC CGCCGCCTGT TCTCCCCGCC GCTGCCGCCG AGGGCCCGGA CCACGCCGAG 
CTGCGCCTGG ACGCCCTGAC CCGTGACAGC AGCGGTAACT ACGAATGCAG CGTCTCCAAC 
GACGTGGGCT CAGCCACCTG CCTCTTCCAG GTCTCCGCTG GGGACCGAAC CCAGGGCCTT 
ACGCTTGCTA GCCACAAGCT GTCCAAGAAC TACTCCTATG TGCTACAGTG GACGCAGAGG 
GAGCCGGACG CCGTGGACCC TGTGCTCAAC TACAGACTCA GCATCCGCCA GTTGAACCAG 
CACAATGCCA TGGTCAAGGC CATCCCTGTT CGGCGTGTGG AGAAGGGGCA GCTGCTGGAG 
TACACCTTGA CTGACCTCCG TGTGCCCCAC AGCTATGAGA TCCACCTCAC ACCCTACACC 
ACCTTTGGGG CTGGAGACAT GGCTTCCCGA GTCATCCACT ACACAGAGCC TATCAACTCG 
CCCAGCCTGT CAGACAATAC CTGTCACTTT GAGGATGAGA AAATCTGCGG ATACACCCAG 
GATCTGACAG ACAACTTCGA CTGGACACGG CAGAATGCGC TTACCCAGAA TCCCAAGCGC 
TCTCCCAATA CTGGGCCACC TACGGACATC AGTGGCACCC CCGAAGGCTA CTACATGTTC 
ATTGAGACAT CAAGACCCCG GGAGCTGGGG GACCGGGCAA GGCTGGTGAG TCCCCTGTAC 
AACGCCAGCG CCAAGTTCTA CTGCGTCTCT TTCTTCTACC ACATGTACGG CAAACACATC 
GGCTCCCTCA ACCTTCTGGT GAGGTCCAGG AACAAAGGCA CTCTAGACAC GCATGCCTGG 
TCCCTCAGCG GTAATAAAGG TAACGTGTGG CAACAGGCCC ACGTGCCCAT CAACCCCAGC 
GGACCCTTCC AGATTATTTT TGAGGGGGTT CGAGGCTCGG GCTACCTGGG GGATATTGCC 
ATAGATGATG TCACACTGAA GAAGGGAGAG TGTCCCCGGA GGCAGATGGA TCCCAACAAA 
GTGGTGGTGA TGCCAGGCAG TGGAGCCCCC CGCCTGTCCA GCCTGCAGCT TTGGGGATCC 
ATGACCATCT TCCTCTTGGC GTTGCAGAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular Mechanism of MDGA1: Regulation of Neuroligin 2:Neurexin Trans-synaptic Bridges.
Neuron94(6)1132-1141.e4(2017 Jun)
Gangwar SP,Zhong X,Seshadrinathan S,Chen H,Machius M,Rudenko G

Interaction between autism-linked MDGAs and neuroligins suppresses inhibitory synapse development.
The Journal of cell biology200(3)321-36(2013 Feb)
Pettem KL,Yokomaku D,Takahashi H,Ge Y,Craig AM

MDGAs interact selectively with neuroligin-2 but not other neuroligins to regulate inhibitory synapse development.
Proceedings of the National Academy of Sciences of the United States of America110(1)336-41(2013 Jan)
Lee K,Kim Y,Lee SJ,Qiang Y,Lee D,Lee HW,Kim H,Je HS,S?dhof TC,Ko J

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
Oncology reports28(2)429-38(2012 Aug)
B?hm D,Keller K,Pieter J,Boehm N,Wolters D,Siggelkow W,Lebrecht A,Schmidt M,K?lbl H,Pfeiffer N,Grus FH

IgSF molecule MDGA1 is involved in radial migration and positioning of a subset of cortical upper-layer neurons.
Developmental dynamics : an official publication of the American Association of Anatomists240(1)96-107(2011 Jan)
Ishikawa T,Gotoh N,Murayama C,Abe T,Iwashita M,Matsuzaki F,Suzuki T,Yamamoto T

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Mdga1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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