Mdga1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mdga1
Entrez Gene ID	74762
Full Name	MAM domain containing glycosylphosphatidylinositol anchor 1
Synonyms	1200011I03Rik,GPIM,Mamdc3
General protein information	Preferred Names MAM domain containing glycosylphosphatidylinositol anchor 1 Names MAM domain-containing glycosylphosphatidylinositol anchor protein 1 mdga1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 A3.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006525041.3	XP_006525104.1	MAM domain-containing glycosylphosphatidylinositol anchor protein 1 isoform X2
XM_006525041.4	XP_006525104.1	MAM domain-containing glycosylphosphatidylinositol anchor protein 1 isoform X1
NM_001368423.1	NP_001355352.1	MAM domain-containing glycosylphosphatidylinositol anchor protein 1 isoform 1 precursor
XM_006525040.3	XP_006525103.1	MAM domain-containing glycosylphosphatidylinositol anchor protein 1 isoform X1
NM_001081160.1	NP_001074629.1	MAM domain-containing glycosylphosphatidylinositol anchor protein 1 isoform 2 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mdga1	NP_001074629.1
Canis lupus familiaris (dog)	MDGA1	XP_532128.3
Danio rerio (zebrafish)	LOC569180	XP_697640.6
Homo sapiens (human)	MDGA1	NP_705691.1
Bos taurus (cattle)	MDGA1	NP_001179811.1
Rattus norvegicus (Norway rat)	Mdga1	NP_001101088.1
Gallus gallus (chicken)	MDGA1	NP_001038112.1
Pan troglodytes (chimpanzee)	MDGA1	XP_527378.2
	MDGA1	XP_001117048.2

Gene Ontology
Bibliography

Related articles in PubMed

Loss of Synapse Repressor MDGA1 Enhances Perisomatic Inhibition, Confers Resistance to Network Excitation, and Impairs Cognitive Function.
Connor SA, Ammendrup-Johnsen I, Kishimoto Y, Karimi Tari P, Cvetkovska V, Harada T, Ojima D, Yamamoto T, Wang YT, Craig AM
Cell reports21(13)3637-3645(2017 Dec)

A dual-strategy expression screen for candidate connectivity labels in the developing thalamus.
Bibollet-Bahena O, Okafuji T, Hokamp K, Tear G, Mitchell KJ
PloS one12(5)e0177977(2017)

Ctip1 Controls Acquisition of Sensory Area Identity and Establishment of Sensory Input Fields in the Developing Neocortex.
Greig LC, Woodworth MB, Greppi C, Macklis JD
Neuron90(2)261-77(2016 04)

Single-cell-level spatial gene expression in the embryonic neural differentiation niche.
Huang Y, Yu X, Sun N, Qiao N, Cao Y, Boyd-Kirkup JD, Shen Q, Han JD
Genome research25(4)570-81(2015 Apr)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mdga1 is involved in the migration and positioning of a subset of cortical neurons and the radial migration of upper-layer neurons might be differentially regulated.
Title: IgSF molecule MDGA1 is involved in radial migration and positioning of a subset of cortical upper-layer neurons.

These findings show that MDGA1 is a unique marker for studies of cortical lamination and area patterning and together with recent reports suggest that MDGA1 has critical functions in forebrain/midbrain development.
Title: Novel IgCAM, MDGA1, expressed in unique cortical area- and layer-specific patterns and transiently by distinct forebrain populations of Cajal-Retzius neurons.

We conclude that MDGA1 acts cell autonomously to control the migration of MDGA1-expressing superficial layer cortical neurons.
Title: Radial migration of superficial layer cortical neurons controlled by novel Ig cell adhesion molecule MDGA1.

The following Mdga1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mdga1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01790	NM_001081160.1 Latest version!	Mus musculus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu54117	NM_001368423.1 Latest version!	Mus musculus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu54117	XM_006525040.3 Latest version!	Mus musculus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu54118	XM_006525041.4 Latest version!	Mus musculus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OMu54118	XM_006525041.3	Mus musculus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mdga1 ( NM_001081160.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074629.1 Mus musculus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu01790
Clone ID Related Accession (Same CDS sequence)	NM_001081160.1
Accession Version	NM_001081160.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	MAM domain-containing glycosylphosphatidylinositol anchor protein 1 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC151294.3 and AC154291.2. On Feb 6, 2007 this sequence version replaced XM_193868.7. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN01164135, SAMN01164137 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGTGA CCTGTCTTCT ACTTCTGGCG CTTATCCCCT TCCACTGTCG GGGACAAGGA 
GTCTATGCTC CAGCCCAGGC CCAGATTGTG CATGCGGGGC AGGCCTGTGT GGTGAAGGAG 
GATAACATCA GCGAGCGGGT CTACACCATC CGGGAGAGCG ACACCCTGGT GCTGCAGTGC 
CTGGTCACGG GACACCCTCG GCCCCAGGTG CGGTGGACCA AAACTGCCGG CAGCGCATCG 
GACAAGTTCC AGGAGACGTC GGTCTTCAAT GAGACTCTGC GGATCGAGCG CATCGCACGC 
ACGCAGGGAG GTCGCTACTA CTGCAAGGCG GAGAACGGAG TGGGCGTGCC GGCCATCAAG 
TCTATCCGCG TGGACGTGCA GTACTTGGAT GAGCCTGTGC TAACGGTGCA TCAGACAGTG 
AGCGATGTCC GAGGCAACTT CTACCAGGAG AAGACCGTGT TTCTACGCTG TACCGTCAGC 
TCCAACCCGC CCGCCCGCTT TATCTGGAAG CGGGGCTCCG ACACCCTGTC CCACAGCCAG 
GATAATGGGG TTGACATCTA CGAGCCTCTC TATACTCAGG GGGAGACGAA GGTCCTGAAG 
CTGAAGAACC TGCGGCCCCA AGACTATGCC AGCTACACCT GCCAGGTGTC CGTGCGCAAT 
GTGTGCGGCA TCCCAGACAA GGCTATCACC TTCCAGCTCA CCAACACCAC GGCACCTCCA 
GCCTTGAAGC TGTCTGTGAA CGAAACTCTG GTGGTGAACC CTGGGGAGAA CGTGACAGTG 
CAGTGTCTGC TGACGGGCGG CGATCCCCTC CCCCAGCTGC ACTGGTCCCA CGGACCTGGC 
CCACTGCCCC TGGGTGCTCT GGCTCAGGGT GGTACCCTCA GCATCCCTTC AGTGCAGGCC 
CGGGATTCTG GCTACTACAA CTGCACAGCC ACCAACAATG TAGGCAACCC AGCTAAGAAG 
ACCGTCAACC TGCTGGTGCG ATCCCTGAAG AACGCCACAT TCCAGATCAC CCCCGACATG 
ATCAAAGAAA GCGAGAACAT TCAACTCGGT CAGGACCTGA AGCTGTCATG CCATGTGGAT 
GCGGTGCCCC AGGAGAAGGT GAACTATCAG TGGTTTAAAA ACGGCAAGCC GGCACGCACA 
TCCAAGCGGC TGCTGGTGAC CCGAAATGAC CCCGAGTTGC CCGCTGTCAC CAGCAGCCTG 
GAGCTCATTG ACCTGCACTT CAGTGACTAC GGCACTTATC TGTGCATGGC TTCCTTCCCA 
GGGTCACCAG TGCCTGACCT CAGCATAGAG GTCAACATTT CCTCAGAGAC AGTACCACCC 
ACCATCAGTG TGCCCAAGGG CCGAGCAGTG GTGACAGTGA GGGAGGGCTC TCCTGCTGAG 
CTCCAGTGCG AAGTTCGTGG CAAGCCCCGG CCTCCAGTGC TATGGTCTCG GGTGGACAAG 
GAGGCCGCCC TTCTGCCCTC GGGGCTGGCC CTGGAGGAGA CTCCGGATGG GAAGCTGCGG 
CTGGAGAGTG TGAGCCGCGA CATGAGCGGC ACCTACCGCT GCCAGACAGC TCGCTACAAT 
GGCTTCAACG TACGACCCCG GGAGGCTCAG GTGCAGCTGA CAGTGCACTT TCCTCCAGAG 
GTGGAGCCCA GTTCCCAGGA CGTGCGCCAG GCGCTGGGCC GGCCTGTGCT CCTGCGCTGC 
TCGCTTCTGC GTGGCAGTCC TCAGCGCATC GCCTCGGCCG TGTGGCGCTT CAAAGGACAG 
CTGCTGCCCC CACCGCCTGT TCTCCCCGCC GCTGCTGTGG AGACCCCCGA TCACGCCGAG 
CTGCGCCTGG ACGCCCTGAC CCGTGACAGC AGCGGCAACT ACGAGTGCAG CGTCTCCAAC 
GACGTGGGCT CAGCCACCTG CCTCTTCCAG GTCTCCGCCA AAGCCTACAG CCCGGAGTTT 
TACTTCGACA CCCCCAACCC CACCCGCAGC CACAAGCTGT CCAAGAATTA CTCCTATGTG 
CTACAGTGGA CACAGAGAGA GCCCGATGCT GTGGACCCCG TGCTCAACTA CAGACTCAGC 
ATCCGACAGT TGAACCAGCA CAATGCCATG GTCAAGGCCA TCCCCGTTCG TCGTGTGGAG 
AAGGGACAGC TGCTGGAGTA TATCTTGACT GACCTCCGAG TACCCCACAG CTATGAGATC 
CGCCTCACAC CCTACACCAC CTTTGGGGCT GGTGACATGG CTTCCCGCAT CATCCACTAC 
ACAGAGCACA ACACCTGTCA CTTTGAGGAT GAGAAAATCT GCGGCTACAC CCAGGATCTG 
ACAGACAACT TTGACTGGAC GCGGCAGAAT GCTCTTACCC AGAACCCCAA GCGCTCTCCT 
AATACTGGGC CCCCTACTGA CATCAGTGGC ACCCCTGAAG GCTATTACAT GTTCATTGAG 
ACATCCAGAC CCAGGGAGCT GGGGGACCGT GCAAGGCTGG TGAGTCCCCT GTACAACGCC 
AGTGCCAAGT TCTACTGTGT CTCTTTCTTC TACCACATGT ATGGGAAACA CATCGGCTCC 
CTCAACCTTC TGGTGCGGTC CAGGAACAAA GGCACTCTAG ACACGCATGC CTGGTCCCTC 
AGTGGCAATA AGGGTAACGT GTGGCAGCAG GCCCATGTAC CCATTAACCC CAGTGGGCCC 
TTCCAGATTA TTTTTGAGGG GGTTCGAGGC TCGGGCTACC TGGGGGATAT TGCCATAGAT 
GATGTCACAC TGAAGAAGGG AGAGTGTCCC CGGAGGCAGA TGGATCCCAA CAAAGTGGTT 
GTGATGCCAG GCAGCGGAGC CCCACGCCTG TCCAGCCTGC AACTTTGGGG ATCCATGGCC 
ATCTTCCTCT TGGCGTTGCA AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074629.1
CDS	370..3216
Translation	MEVTCLLLLALIPFHCRGQGVYAPAQAQIVHAGQACVVKEDNISERVYTIRESDTLVLQCLVTGHPRPQVRWTKTAGSASDKFQETSVFNETLRIERIARTQGGRYYCKAENGVGVPAIKSIRVDVQYLDEPVLTVHQTVSDVRGNFYQEKTVFLRCTVSSNPPARFIWKRGSDTLSHSQDNGVDIYEPLYTQGETKVLKLKNLRPQDYASYTCQVSVRNVCGIPDKAITFQLTNTTAPPALKLSVNETLVVNPGENVTVQCLLTGGDPLPQLHWSHGPGPLPLGALAQGGTLSIPSVQARDSGYYNCTATNNVGNPAKKTVNLLVRSLKNATFQITPDMIKESENIQLGQDLKLSCHVDAVPQEKVNYQWFKNGKPARTSKRLLVTRNDPELPAVTSSLELIDLHFSDYGTYLCMASFPGSPVPDLSIEVNISSETVPPTISVPKGRAVVTVREGSPAELQCEVRGKPRPPVLWSRVDKEAALLPSGLALEETPDGKLRLESVSRDMSGTYRCQTARYNGFNVRPREAQVQLTVHFPPEVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVLPAAAVETPDHAELRLDALTRDSSGNYECSVSNDVGSATCLFQVSAKAYSPEFYFDTPNPTRSHKLSKNYSYVLQWTQREPDAVDPVLNYRLSIRQLNQHNAMVKAIPVRRVEKGQLLEYILTDLRVPHSYEIRLTPYTTFGAGDMASRIIHYTEHNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPEGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGTLDTHAWSLSGNKGNVWQQAHVPINPSGPFQIIFEGVRGSGYLGDIAIDDVTLKKGECPRRQMDPNKVVVMPGSGAPRLSSLQLWGSMAIFLLALQR

Target ORF information:

RefSeq Version	NM_001081160.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081160.1

ORF Insert Sequence:

ATGGAGGTGA CCTGTCTTCT ACTTCTGGCG CTTATCCCCT TCCACTGTCG GGGACAAGGA 
GTCTATGCTC CAGCCCAGGC CCAGATTGTG CATGCGGGGC AGGCCTGTGT GGTGAAGGAG 
GATAACATCA GCGAGCGGGT CTACACCATC CGGGAGAGCG ACACCCTGGT GCTGCAGTGC 
CTGGTCACGG GACACCCTCG GCCCCAGGTG CGGTGGACCA AAACTGCCGG CAGCGCATCG 
GACAAGTTCC AGGAGACGTC GGTCTTCAAT GAGACTCTGC GGATCGAGCG CATCGCACGC 
ACGCAGGGAG GTCGCTACTA CTGCAAGGCG GAGAACGGAG TGGGCGTGCC GGCCATCAAG 
TCTATCCGCG TGGACGTGCA GTACTTGGAT GAGCCTGTGC TAACGGTGCA TCAGACAGTG 
AGCGATGTCC GAGGCAACTT CTACCAGGAG AAGACCGTGT TTCTACGCTG TACCGTCAGC 
TCCAACCCGC CCGCCCGCTT TATCTGGAAG CGGGGCTCCG ACACCCTGTC CCACAGCCAG 
GATAATGGGG TTGACATCTA CGAGCCTCTC TATACTCAGG GGGAGACGAA GGTCCTGAAG 
CTGAAGAACC TGCGGCCCCA AGACTATGCC AGCTACACCT GCCAGGTGTC CGTGCGCAAT 
GTGTGCGGCA TCCCAGACAA GGCTATCACC TTCCAGCTCA CCAACACCAC GGCACCTCCA 
GCCTTGAAGC TGTCTGTGAA CGAAACTCTG GTGGTGAACC CTGGGGAGAA CGTGACAGTG 
CAGTGTCTGC TGACGGGCGG CGATCCCCTC CCCCAGCTGC ACTGGTCCCA CGGACCTGGC 
CCACTGCCCC TGGGTGCTCT GGCTCAGGGT GGTACCCTCA GCATCCCTTC AGTGCAGGCC 
CGGGATTCTG GCTACTACAA CTGCACAGCC ACCAACAATG TAGGCAACCC AGCTAAGAAG 
ACCGTCAACC TGCTGGTGCG ATCCCTGAAG AACGCCACAT TCCAGATCAC CCCCGACATG 
ATCAAAGAAA GCGAGAACAT TCAACTCGGT CAGGACCTGA AGCTGTCATG CCATGTGGAT 
GCGGTGCCCC AGGAGAAGGT GAACTATCAG TGGTTTAAAA ACGGCAAGCC GGCACGCACA 
TCCAAGCGGC TGCTGGTGAC CCGAAATGAC CCCGAGTTGC CCGCTGTCAC CAGCAGCCTG 
GAGCTCATTG ACCTGCACTT CAGTGACTAC GGCACTTATC TGTGCATGGC TTCCTTCCCA 
GGGTCACCAG TGCCTGACCT CAGCATAGAG GTCAACATTT CCTCAGAGAC AGTACCACCC 
ACCATCAGTG TGCCCAAGGG CCGAGCAGTG GTGACAGTGA GGGAGGGCTC TCCTGCTGAG 
CTCCAGTGCG AAGTTCGTGG CAAGCCCCGG CCTCCAGTGC TATGGTCTCG GGTGGACAAG 
GAGGCCGCCC TTCTGCCCTC GGGGCTGGCC CTGGAGGAGA CTCCGGATGG GAAGCTGCGG 
CTGGAGAGTG TGAGCCGCGA CATGAGCGGC ACCTACCGCT GCCAGACAGC TCGCTACAAT 
GGCTTCAACG TACGACCCCG GGAGGCTCAG GTGCAGCTGA CAGTGCACTT TCCTCCAGAG 
GTGGAGCCCA GTTCCCAGGA CGTGCGCCAG GCGCTGGGCC GGCCTGTGCT CCTGCGCTGC 
TCGCTTCTGC GTGGCAGTCC TCAGCGCATC GCCTCGGCCG TGTGGCGCTT CAAAGGACAG 
CTGCTGCCCC CACCGCCTGT TCTCCCCGCC GCTGCTGTGG AGACCCCCGA TCACGCCGAG 
CTGCGCCTGG ACGCCCTGAC CCGTGACAGC AGCGGCAACT ACGAGTGCAG CGTCTCCAAC 
GACGTGGGCT CAGCCACCTG CCTCTTCCAG GTCTCCGCCA AAGCCTACAG CCCGGAGTTT 
TACTTCGACA CCCCCAACCC CACCCGCAGC CACAAGCTGT CCAAGAATTA CTCCTATGTG 
CTACAGTGGA CACAGAGAGA GCCCGATGCT GTGGACCCCG TGCTCAACTA CAGACTCAGC 
ATCCGACAGT TGAACCAGCA CAATGCCATG GTCAAGGCCA TCCCCGTTCG TCGTGTGGAG 
AAGGGACAGC TGCTGGAGTA TATCTTGACT GACCTCCGAG TACCCCACAG CTATGAGATC 
CGCCTCACAC CCTACACCAC CTTTGGGGCT GGTGACATGG CTTCCCGCAT CATCCACTAC 
ACAGAGCACA ACACCTGTCA CTTTGAGGAT GAGAAAATCT GCGGCTACAC CCAGGATCTG 
ACAGACAACT TTGACTGGAC GCGGCAGAAT GCTCTTACCC AGAACCCCAA GCGCTCTCCT 
AATACTGGGC CCCCTACTGA CATCAGTGGC ACCCCTGAAG GCTATTACAT GTTCATTGAG 
ACATCCAGAC CCAGGGAGCT GGGGGACCGT GCAAGGCTGG TGAGTCCCCT GTACAACGCC 
AGTGCCAAGT TCTACTGTGT CTCTTTCTTC TACCACATGT ATGGGAAACA CATCGGCTCC 
CTCAACCTTC TGGTGCGGTC CAGGAACAAA GGCACTCTAG ACACGCATGC CTGGTCCCTC 
AGTGGCAATA AGGGTAACGT GTGGCAGCAG GCCCATGTAC CCATTAACCC CAGTGGGCCC 
TTCCAGATTA TTTTTGAGGG GGTTCGAGGC TCGGGCTACC TGGGGGATAT TGCCATAGAT 
GATGTCACAC TGAAGAAGGG AGAGTGTCCC CGGAGGCAGA TGGATCCCAA CAAAGTGGTT 
GTGATGCCAG GCAGCGGAGC CCCACGCCTG TCCAGCCTGC AACTTTGGGG ATCCATGGCC 
ATCTTCCTCT TGGCGTTGCA AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdga1 ( NM_001368423.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001355352.1 Mus musculus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu54117
Clone ID Related Accession (Same CDS sequence)	NM_001368423.1 , XM_006525040.3
Accession Version	NM_001368423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	MAM domain-containing glycosylphosphatidylinositol anchor protein 1 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC151294.3 and AC154291.2. On Feb 13, 2019 this sequence version replaced XM_006525040.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.987597.1, SRR7345562.1814199.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGTGA CCTGTCTTCT ACTTCTGGCG CTTATCCCCT TCCACTGTCG GGGACAAGGA 
GTCTATGCTC CAGCCCAGGC CCAGATTGTG CATGCGGGGC AGGCCTGTGT GGTGAAGGAG 
GATAACATCA GCGAGCGGGT CTACACCATC CGGGAGAGCG ACACCCTGGT GCTGCAGTGC 
CTGGTCACGG GACACCCTCG GCCCCAGGTG CGGTGGACCA AAACTGCCGG CAGCGCATCG 
GACAAGTTCC AGGAGACGTC GGTCTTCAAT GAGACTCTGC GGATCGAGCG CATCGCACGC 
ACGCAGGGAG GTCGCTACTA CTGCAAGGCG GAGAACGGAG TGGGCGTGCC GGCCATCAAG 
TCTATCCGCG TGGACGTGCA GTACTTGGAT GAGCCTGTGC TAACGGTGCA TCAGACAGTG 
AGCGATGTCC GAGGCAACTT CTACCAGGAG AAGACCGTGT TTCTACGCTG TACCGTCAGC 
TCCAACCCGC CCGCCCGCTT TATCTGGAAG CGGGGCTCCG ACACCCTGTC CCACAGCCAG 
GATAATGGGG TTGACATCTA CGAGCCTCTC TATACTCAGG GGGAGACGAA GGTCCTGAAG 
CTGAAGAACC TGCGGCCCCA AGACTATGCC AGCTACACCT GCCAGGTGTC CGTGCGCAAT 
GTGTGCGGCA TCCCAGACAA GGCTATCACC TTCCAGCTCA CCAACACCAC GGCACCTCCA 
GCCTTGAAGC TGTCTGTGAA CGAAACTCTG GTGGTGAACC CTGGGGAGAA CGTGACAGTG 
CAGTGTCTGC TGACGGGCGG CGATCCCCTC CCCCAGCTGC ACTGGTCCCA CGGACCTGGC 
CCACTGCCCC TGGGTGCTCT GGCTCAGGGT GGTACCCTCA GCATCCCTTC AGTGCAGGCC 
CGGGATTCTG GCTACTACAA CTGCACAGCC ACCAACAATG TAGGCAACCC AGCTAAGAAG 
ACCGTCAACC TGCTGGTGCG ATCCCTGAAG AACGCCACAT TCCAGATCAC CCCCGACATG 
ATCAAAGAAA GCGAGAACAT TCAACTCGGT CAGGACCTGA AGCTGTCATG CCATGTGGAT 
GCGGTGCCCC AGGAGAAGGT GAACTATCAG TGGTTTAAAA ACGGCAAGCC GGCACGCACA 
TCCAAGCGGC TGCTGGTGAC CCGAAATGAC CCCGAGTTGC CCGCTGTCAC CAGCAGCCTG 
GAGCTCATTG ACCTGCACTT CAGTGACTAC GGCACTTATC TGTGCATGGC TTCCTTCCCA 
GGGTCACCAG TGCCTGACCT CAGCATAGAG GTCAACATTT CCTCAGAGAC AGTACCACCC 
ACCATCAGTG TGCCCAAGGG CCGAGCAGTG GTGACAGTGA GGGAGGGCTC TCCTGCTGAG 
CTCCAGTGCG AAGTTCGTGG CAAGCCCCGG CCTCCAGTGC TATGGTCTCG GGTGGACAAG 
GAGGCCGCCC TTCTGCCCTC GGGGCTGGCC CTGGAGGAGA CTCCGGATGG GAAGCTGCGG 
CTGGAGAGTG TGAGCCGCGA CATGAGCGGC ACCTACCGCT GCCAGACAGC TCGCTACAAT 
GGCTTCAACG TACGACCCCG GGAGGCTCAG GTGCAGCTGA CAGTGCACTT TCCTCCAGAG 
GTGGAGCCCA GTTCCCAGGA CGTGCGCCAG GCGCTGGGCC GGCCTGTGCT CCTGCGCTGC 
TCGCTTCTGC GTGGCAGTCC TCAGCGCATC GCCTCGGCCG TGTGGCGCTT CAAAGGACAG 
CTGCTGCCCC CACCGCCTGT TCTCCCCGCC GCTGCTGTGG AGACCCCCGA TCACGCCGAG 
CTGCGCCTGG ACGCCCTGAC CCGTGACAGC AGCGGCAACT ACGAGTGCAG CGTCTCCAAC 
GACGTGGGCT CAGCCACCTG CCTCTTCCAG GTCTCCGCCA AAGCCTACAG CCCGGAGTTT 
TACTTCGACA CCCCCAACCC CACCCGCAGC CACAAGCTGT CCAAGAATTA CTCCTATGTG 
CTACAGTGGA CACAGAGAGA GCCCGATGCT GTGGACCCCG TGCTCAACTA CAGACTCAGC 
ATCCGACAGT TGAACCAGCA CAATGCCATG GTCAAGGCCA TCCCCGTTCG TCGTGTGGAG 
AAGGGACAGC TGCTGGAGTA TATCTTGACT GACCTCCGAG TACCCCACAG CTATGAGATC 
CGCCTCACAC CCTACACCAC CTTTGGGGCT GGTGACATGG CTTCCCGCAT CATCCACTAC 
ACAGAGCCTA TCAACTTGCC CAGCCTGTCA GACAACACCT GTCACTTTGA GGATGAGAAA 
ATCTGCGGCT ACACCCAGGA TCTGACAGAC AACTTTGACT GGACGCGGCA GAATGCTCTT 
ACCCAGAACC CCAAGCGCTC TCCTAATACT GGGCCCCCTA CTGACATCAG TGGCACCCCT 
GAAGGCTATT ACATGTTCAT TGAGACATCC AGACCCAGGG AGCTGGGGGA CCGTGCAAGG 
CTGGTGAGTC CCCTGTACAA CGCCAGTGCC AAGTTCTACT GTGTCTCTTT CTTCTACCAC 
ATGTATGGGA AACACATCGG CTCCCTCAAC CTTCTGGTGC GGTCCAGGAA CAAAGGCACT 
CTAGACACGC ATGCCTGGTC CCTCAGTGGC AATAAGGGTA ACGTGTGGCA GCAGGCCCAT 
GTACCCATTA ACCCCAGTGG GCCCTTCCAG ATTATTTTTG AGGGGGTTCG AGGCTCGGGC 
TACCTGGGGG ATATTGCCAT AGATGATGTC ACACTGAAGA AGGGAGAGTG TCCCCGGAGG 
CAGATGGATC CCAACAAAGT GGTTGTGATG CCAGGCAGCG GAGCCCCACG CCTGTCCAGC 
CTGCAACTTT GGGGATCCAT GGCCATCTTC CTCTTGGCGT TGCAAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355352.1
CDS	1066..3936
Translation	MEVTCLLLLALIPFHCRGQGVYAPAQAQIVHAGQACVVKEDNISERVYTIRESDTLVLQCLVTGHPRPQVRWTKTAGSASDKFQETSVFNETLRIERIARTQGGRYYCKAENGVGVPAIKSIRVDVQYLDEPVLTVHQTVSDVRGNFYQEKTVFLRCTVSSNPPARFIWKRGSDTLSHSQDNGVDIYEPLYTQGETKVLKLKNLRPQDYASYTCQVSVRNVCGIPDKAITFQLTNTTAPPALKLSVNETLVVNPGENVTVQCLLTGGDPLPQLHWSHGPGPLPLGALAQGGTLSIPSVQARDSGYYNCTATNNVGNPAKKTVNLLVRSLKNATFQITPDMIKESENIQLGQDLKLSCHVDAVPQEKVNYQWFKNGKPARTSKRLLVTRNDPELPAVTSSLELIDLHFSDYGTYLCMASFPGSPVPDLSIEVNISSETVPPTISVPKGRAVVTVREGSPAELQCEVRGKPRPPVLWSRVDKEAALLPSGLALEETPDGKLRLESVSRDMSGTYRCQTARYNGFNVRPREAQVQLTVHFPPEVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVLPAAAVETPDHAELRLDALTRDSSGNYECSVSNDVGSATCLFQVSAKAYSPEFYFDTPNPTRSHKLSKNYSYVLQWTQREPDAVDPVLNYRLSIRQLNQHNAMVKAIPVRRVEKGQLLEYILTDLRVPHSYEIRLTPYTTFGAGDMASRIIHYTEPINLPSLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPEGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGTLDTHAWSLSGNKGNVWQQAHVPINPSGPFQIIFEGVRGSGYLGDIAIDDVTLKKGECPRRQMDPNKVVVMPGSGAPRLSSLQLWGSMAIFLLALQR

Target ORF information:

RefSeq Version	NM_001368423.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368423.1

ORF Insert Sequence:

ATGGAGGTGA CCTGTCTTCT ACTTCTGGCG CTTATCCCCT TCCACTGTCG GGGACAAGGA 
GTCTATGCTC CAGCCCAGGC CCAGATTGTG CATGCGGGGC AGGCCTGTGT GGTGAAGGAG 
GATAACATCA GCGAGCGGGT CTACACCATC CGGGAGAGCG ACACCCTGGT GCTGCAGTGC 
CTGGTCACGG GACACCCTCG GCCCCAGGTG CGGTGGACCA AAACTGCCGG CAGCGCATCG 
GACAAGTTCC AGGAGACGTC GGTCTTCAAT GAGACTCTGC GGATCGAGCG CATCGCACGC 
ACGCAGGGAG GTCGCTACTA CTGCAAGGCG GAGAACGGAG TGGGCGTGCC GGCCATCAAG 
TCTATCCGCG TGGACGTGCA GTACTTGGAT GAGCCTGTGC TAACGGTGCA TCAGACAGTG 
AGCGATGTCC GAGGCAACTT CTACCAGGAG AAGACCGTGT TTCTACGCTG TACCGTCAGC 
TCCAACCCGC CCGCCCGCTT TATCTGGAAG CGGGGCTCCG ACACCCTGTC CCACAGCCAG 
GATAATGGGG TTGACATCTA CGAGCCTCTC TATACTCAGG GGGAGACGAA GGTCCTGAAG 
CTGAAGAACC TGCGGCCCCA AGACTATGCC AGCTACACCT GCCAGGTGTC CGTGCGCAAT 
GTGTGCGGCA TCCCAGACAA GGCTATCACC TTCCAGCTCA CCAACACCAC GGCACCTCCA 
GCCTTGAAGC TGTCTGTGAA CGAAACTCTG GTGGTGAACC CTGGGGAGAA CGTGACAGTG 
CAGTGTCTGC TGACGGGCGG CGATCCCCTC CCCCAGCTGC ACTGGTCCCA CGGACCTGGC 
CCACTGCCCC TGGGTGCTCT GGCTCAGGGT GGTACCCTCA GCATCCCTTC AGTGCAGGCC 
CGGGATTCTG GCTACTACAA CTGCACAGCC ACCAACAATG TAGGCAACCC AGCTAAGAAG 
ACCGTCAACC TGCTGGTGCG ATCCCTGAAG AACGCCACAT TCCAGATCAC CCCCGACATG 
ATCAAAGAAA GCGAGAACAT TCAACTCGGT CAGGACCTGA AGCTGTCATG CCATGTGGAT 
GCGGTGCCCC AGGAGAAGGT GAACTATCAG TGGTTTAAAA ACGGCAAGCC GGCACGCACA 
TCCAAGCGGC TGCTGGTGAC CCGAAATGAC CCCGAGTTGC CCGCTGTCAC CAGCAGCCTG 
GAGCTCATTG ACCTGCACTT CAGTGACTAC GGCACTTATC TGTGCATGGC TTCCTTCCCA 
GGGTCACCAG TGCCTGACCT CAGCATAGAG GTCAACATTT CCTCAGAGAC AGTACCACCC 
ACCATCAGTG TGCCCAAGGG CCGAGCAGTG GTGACAGTGA GGGAGGGCTC TCCTGCTGAG 
CTCCAGTGCG AAGTTCGTGG CAAGCCCCGG CCTCCAGTGC TATGGTCTCG GGTGGACAAG 
GAGGCCGCCC TTCTGCCCTC GGGGCTGGCC CTGGAGGAGA CTCCGGATGG GAAGCTGCGG 
CTGGAGAGTG TGAGCCGCGA CATGAGCGGC ACCTACCGCT GCCAGACAGC TCGCTACAAT 
GGCTTCAACG TACGACCCCG GGAGGCTCAG GTGCAGCTGA CAGTGCACTT TCCTCCAGAG 
GTGGAGCCCA GTTCCCAGGA CGTGCGCCAG GCGCTGGGCC GGCCTGTGCT CCTGCGCTGC 
TCGCTTCTGC GTGGCAGTCC TCAGCGCATC GCCTCGGCCG TGTGGCGCTT CAAAGGACAG 
CTGCTGCCCC CACCGCCTGT TCTCCCCGCC GCTGCTGTGG AGACCCCCGA TCACGCCGAG 
CTGCGCCTGG ACGCCCTGAC CCGTGACAGC AGCGGCAACT ACGAGTGCAG CGTCTCCAAC 
GACGTGGGCT CAGCCACCTG CCTCTTCCAG GTCTCCGCCA AAGCCTACAG CCCGGAGTTT 
TACTTCGACA CCCCCAACCC CACCCGCAGC CACAAGCTGT CCAAGAATTA CTCCTATGTG 
CTACAGTGGA CACAGAGAGA GCCCGATGCT GTGGACCCCG TGCTCAACTA CAGACTCAGC 
ATCCGACAGT TGAACCAGCA CAATGCCATG GTCAAGGCCA TCCCCGTTCG TCGTGTGGAG 
AAGGGACAGC TGCTGGAGTA TATCTTGACT GACCTCCGAG TACCCCACAG CTATGAGATC 
CGCCTCACAC CCTACACCAC CTTTGGGGCT GGTGACATGG CTTCCCGCAT CATCCACTAC 
ACAGAGCCTA TCAACTTGCC CAGCCTGTCA GACAACACCT GTCACTTTGA GGATGAGAAA 
ATCTGCGGCT ACACCCAGGA TCTGACAGAC AACTTTGACT GGACGCGGCA GAATGCTCTT 
ACCCAGAACC CCAAGCGCTC TCCTAATACT GGGCCCCCTA CTGACATCAG TGGCACCCCT 
GAAGGCTATT ACATGTTCAT TGAGACATCC AGACCCAGGG AGCTGGGGGA CCGTGCAAGG 
CTGGTGAGTC CCCTGTACAA CGCCAGTGCC AAGTTCTACT GTGTCTCTTT CTTCTACCAC 
ATGTATGGGA AACACATCGG CTCCCTCAAC CTTCTGGTGC GGTCCAGGAA CAAAGGCACT 
CTAGACACGC ATGCCTGGTC CCTCAGTGGC AATAAGGGTA ACGTGTGGCA GCAGGCCCAT 
GTACCCATTA ACCCCAGTGG GCCCTTCCAG ATTATTTTTG AGGGGGTTCG AGGCTCGGGC 
TACCTGGGGG ATATTGCCAT AGATGATGTC ACACTGAAGA AGGGAGAGTG TCCCCGGAGG 
CAGATGGATC CCAACAAAGT GGTTGTGATG CCAGGCAGCG GAGCCCCACG CCTGTCCAGC 
CTGCAACTTT GGGGATCCAT GGCCATCTTC CTCTTGGCGT TGCAAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdga1 ( XM_006525040.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525103.1 Mus musculus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), transcript variant X1, mRNA.

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CloneID	OMu54117
Clone ID Related Accession (Same CDS sequence)	NM_001368423.1 , XM_006525040.3
Accession Version	XM_006525040.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	MAM domain-containing glycosylphosphatidylinositol anchor protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525040.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGA CCTGTCTTCT ACTTCTGGCG CTTATCCCCT TCCACTGTCG GGGACAAGGA 
GTCTATGCTC CAGCCCAGGC CCAGATTGTG CATGCGGGGC AGGCCTGTGT GGTGAAGGAG 
GATAACATCA GCGAGCGGGT CTACACCATC CGGGAGAGCG ACACCCTGGT GCTGCAGTGC 
CTGGTCACGG GACACCCTCG GCCCCAGGTG CGGTGGACCA AAACTGCCGG CAGCGCATCG 
GACAAGTTCC AGGAGACGTC GGTCTTCAAT GAGACTCTGC GGATCGAGCG CATCGCACGC 
ACGCAGGGAG GTCGCTACTA CTGCAAGGCG GAGAACGGAG TGGGCGTGCC GGCCATCAAG 
TCTATCCGCG TGGACGTGCA GTACTTGGAT GAGCCTGTGC TAACGGTGCA TCAGACAGTG 
AGCGATGTCC GAGGCAACTT CTACCAGGAG AAGACCGTGT TTCTACGCTG TACCGTCAGC 
TCCAACCCGC CCGCCCGCTT TATCTGGAAG CGGGGCTCCG ACACCCTGTC CCACAGCCAG 
GATAATGGGG TTGACATCTA CGAGCCTCTC TATACTCAGG GGGAGACGAA GGTCCTGAAG 
CTGAAGAACC TGCGGCCCCA AGACTATGCC AGCTACACCT GCCAGGTGTC CGTGCGCAAT 
GTGTGCGGCA TCCCAGACAA GGCTATCACC TTCCAGCTCA CCAACACCAC GGCACCTCCA 
GCCTTGAAGC TGTCTGTGAA CGAAACTCTG GTGGTGAACC CTGGGGAGAA CGTGACAGTG 
CAGTGTCTGC TGACGGGCGG CGATCCCCTC CCCCAGCTGC ACTGGTCCCA CGGACCTGGC 
CCACTGCCCC TGGGTGCTCT GGCTCAGGGT GGTACCCTCA GCATCCCTTC AGTGCAGGCC 
CGGGATTCTG GCTACTACAA CTGCACAGCC ACCAACAATG TAGGCAACCC AGCTAAGAAG 
ACCGTCAACC TGCTGGTGCG ATCCCTGAAG AACGCCACAT TCCAGATCAC CCCCGACATG 
ATCAAAGAAA GCGAGAACAT TCAACTCGGT CAGGACCTGA AGCTGTCATG CCATGTGGAT 
GCGGTGCCCC AGGAGAAGGT GAACTATCAG TGGTTTAAAA ACGGCAAGCC GGCACGCACA 
TCCAAGCGGC TGCTGGTGAC CCGAAATGAC CCCGAGTTGC CCGCTGTCAC CAGCAGCCTG 
GAGCTCATTG ACCTGCACTT CAGTGACTAC GGCACTTATC TGTGCATGGC TTCCTTCCCA 
GGGTCACCAG TGCCTGACCT CAGCATAGAG GTCAACATTT CCTCAGAGAC AGTACCACCC 
ACCATCAGTG TGCCCAAGGG CCGAGCAGTG GTGACAGTGA GGGAGGGCTC TCCTGCTGAG 
CTCCAGTGCG AAGTTCGTGG CAAGCCCCGG CCTCCAGTGC TATGGTCTCG GGTGGACAAG 
GAGGCCGCCC TTCTGCCCTC GGGGCTGGCC CTGGAGGAGA CTCCGGATGG GAAGCTGCGG 
CTGGAGAGTG TGAGCCGCGA CATGAGCGGC ACCTACCGCT GCCAGACAGC TCGCTACAAT 
GGCTTCAACG TACGACCCCG GGAGGCTCAG GTGCAGCTGA CAGTGCACTT TCCTCCAGAG 
GTGGAGCCCA GTTCCCAGGA CGTGCGCCAG GCGCTGGGCC GGCCTGTGCT CCTGCGCTGC 
TCGCTTCTGC GTGGCAGTCC TCAGCGCATC GCCTCGGCCG TGTGGCGCTT CAAAGGACAG 
CTGCTGCCCC CACCGCCTGT TCTCCCCGCC GCTGCTGTGG AGACCCCCGA TCACGCCGAG 
CTGCGCCTGG ACGCCCTGAC CCGTGACAGC AGCGGCAACT ACGAGTGCAG CGTCTCCAAC 
GACGTGGGCT CAGCCACCTG CCTCTTCCAG GTCTCCGCCA AAGCCTACAG CCCGGAGTTT 
TACTTCGACA CCCCCAACCC CACCCGCAGC CACAAGCTGT CCAAGAATTA CTCCTATGTG 
CTACAGTGGA CACAGAGAGA GCCCGATGCT GTGGACCCCG TGCTCAACTA CAGACTCAGC 
ATCCGACAGT TGAACCAGCA CAATGCCATG GTCAAGGCCA TCCCCGTTCG TCGTGTGGAG 
AAGGGACAGC TGCTGGAGTA TATCTTGACT GACCTCCGAG TACCCCACAG CTATGAGATC 
CGCCTCACAC CCTACACCAC CTTTGGGGCT GGTGACATGG CTTCCCGCAT CATCCACTAC 
ACAGAGCCTA TCAACTTGCC CAGCCTGTCA GACAACACCT GTCACTTTGA GGATGAGAAA 
ATCTGCGGCT ACACCCAGGA TCTGACAGAC AACTTTGACT GGACGCGGCA GAATGCTCTT 
ACCCAGAACC CCAAGCGCTC TCCTAATACT GGGCCCCCTA CTGACATCAG TGGCACCCCT 
GAAGGCTATT ACATGTTCAT TGAGACATCC AGACCCAGGG AGCTGGGGGA CCGTGCAAGG 
CTGGTGAGTC CCCTGTACAA CGCCAGTGCC AAGTTCTACT GTGTCTCTTT CTTCTACCAC 
ATGTATGGGA AACACATCGG CTCCCTCAAC CTTCTGGTGC GGTCCAGGAA CAAAGGCACT 
CTAGACACGC ATGCCTGGTC CCTCAGTGGC AATAAGGGTA ACGTGTGGCA GCAGGCCCAT 
GTACCCATTA ACCCCAGTGG GCCCTTCCAG ATTATTTTTG AGGGGGTTCG AGGCTCGGGC 
TACCTGGGGG ATATTGCCAT AGATGATGTC ACACTGAAGA AGGGAGAGTG TCCCCGGAGG 
CAGATGGATC CCAACAAAGT GGTTGTGATG CCAGGCAGCG GAGCCCCACG CCTGTCCAGC 
CTGCAACTTT GGGGATCCAT GGCCATCTTC CTCTTGGCGT TGCAAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525103.1
CDS	913..3783
Translation	MEVTCLLLLALIPFHCRGQGVYAPAQAQIVHAGQACVVKEDNISERVYTIRESDTLVLQCLVTGHPRPQVRWTKTAGSASDKFQETSVFNETLRIERIARTQGGRYYCKAENGVGVPAIKSIRVDVQYLDEPVLTVHQTVSDVRGNFYQEKTVFLRCTVSSNPPARFIWKRGSDTLSHSQDNGVDIYEPLYTQGETKVLKLKNLRPQDYASYTCQVSVRNVCGIPDKAITFQLTNTTAPPALKLSVNETLVVNPGENVTVQCLLTGGDPLPQLHWSHGPGPLPLGALAQGGTLSIPSVQARDSGYYNCTATNNVGNPAKKTVNLLVRSLKNATFQITPDMIKESENIQLGQDLKLSCHVDAVPQEKVNYQWFKNGKPARTSKRLLVTRNDPELPAVTSSLELIDLHFSDYGTYLCMASFPGSPVPDLSIEVNISSETVPPTISVPKGRAVVTVREGSPAELQCEVRGKPRPPVLWSRVDKEAALLPSGLALEETPDGKLRLESVSRDMSGTYRCQTARYNGFNVRPREAQVQLTVHFPPEVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVLPAAAVETPDHAELRLDALTRDSSGNYECSVSNDVGSATCLFQVSAKAYSPEFYFDTPNPTRSHKLSKNYSYVLQWTQREPDAVDPVLNYRLSIRQLNQHNAMVKAIPVRRVEKGQLLEYILTDLRVPHSYEIRLTPYTTFGAGDMASRIIHYTEPINLPSLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPEGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGTLDTHAWSLSGNKGNVWQQAHVPINPSGPFQIIFEGVRGSGYLGDIAIDDVTLKKGECPRRQMDPNKVVVMPGSGAPRLSSLQLWGSMAIFLLALQR

Target ORF information:

RefSeq Version	XM_006525040.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525040.3

ORF Insert Sequence:

ATGGAGGTGA CCTGTCTTCT ACTTCTGGCG CTTATCCCCT TCCACTGTCG GGGACAAGGA 
GTCTATGCTC CAGCCCAGGC CCAGATTGTG CATGCGGGGC AGGCCTGTGT GGTGAAGGAG 
GATAACATCA GCGAGCGGGT CTACACCATC CGGGAGAGCG ACACCCTGGT GCTGCAGTGC 
CTGGTCACGG GACACCCTCG GCCCCAGGTG CGGTGGACCA AAACTGCCGG CAGCGCATCG 
GACAAGTTCC AGGAGACGTC GGTCTTCAAT GAGACTCTGC GGATCGAGCG CATCGCACGC 
ACGCAGGGAG GTCGCTACTA CTGCAAGGCG GAGAACGGAG TGGGCGTGCC GGCCATCAAG 
TCTATCCGCG TGGACGTGCA GTACTTGGAT GAGCCTGTGC TAACGGTGCA TCAGACAGTG 
AGCGATGTCC GAGGCAACTT CTACCAGGAG AAGACCGTGT TTCTACGCTG TACCGTCAGC 
TCCAACCCGC CCGCCCGCTT TATCTGGAAG CGGGGCTCCG ACACCCTGTC CCACAGCCAG 
GATAATGGGG TTGACATCTA CGAGCCTCTC TATACTCAGG GGGAGACGAA GGTCCTGAAG 
CTGAAGAACC TGCGGCCCCA AGACTATGCC AGCTACACCT GCCAGGTGTC CGTGCGCAAT 
GTGTGCGGCA TCCCAGACAA GGCTATCACC TTCCAGCTCA CCAACACCAC GGCACCTCCA 
GCCTTGAAGC TGTCTGTGAA CGAAACTCTG GTGGTGAACC CTGGGGAGAA CGTGACAGTG 
CAGTGTCTGC TGACGGGCGG CGATCCCCTC CCCCAGCTGC ACTGGTCCCA CGGACCTGGC 
CCACTGCCCC TGGGTGCTCT GGCTCAGGGT GGTACCCTCA GCATCCCTTC AGTGCAGGCC 
CGGGATTCTG GCTACTACAA CTGCACAGCC ACCAACAATG TAGGCAACCC AGCTAAGAAG 
ACCGTCAACC TGCTGGTGCG ATCCCTGAAG AACGCCACAT TCCAGATCAC CCCCGACATG 
ATCAAAGAAA GCGAGAACAT TCAACTCGGT CAGGACCTGA AGCTGTCATG CCATGTGGAT 
GCGGTGCCCC AGGAGAAGGT GAACTATCAG TGGTTTAAAA ACGGCAAGCC GGCACGCACA 
TCCAAGCGGC TGCTGGTGAC CCGAAATGAC CCCGAGTTGC CCGCTGTCAC CAGCAGCCTG 
GAGCTCATTG ACCTGCACTT CAGTGACTAC GGCACTTATC TGTGCATGGC TTCCTTCCCA 
GGGTCACCAG TGCCTGACCT CAGCATAGAG GTCAACATTT CCTCAGAGAC AGTACCACCC 
ACCATCAGTG TGCCCAAGGG CCGAGCAGTG GTGACAGTGA GGGAGGGCTC TCCTGCTGAG 
CTCCAGTGCG AAGTTCGTGG CAAGCCCCGG CCTCCAGTGC TATGGTCTCG GGTGGACAAG 
GAGGCCGCCC TTCTGCCCTC GGGGCTGGCC CTGGAGGAGA CTCCGGATGG GAAGCTGCGG 
CTGGAGAGTG TGAGCCGCGA CATGAGCGGC ACCTACCGCT GCCAGACAGC TCGCTACAAT 
GGCTTCAACG TACGACCCCG GGAGGCTCAG GTGCAGCTGA CAGTGCACTT TCCTCCAGAG 
GTGGAGCCCA GTTCCCAGGA CGTGCGCCAG GCGCTGGGCC GGCCTGTGCT CCTGCGCTGC 
TCGCTTCTGC GTGGCAGTCC TCAGCGCATC GCCTCGGCCG TGTGGCGCTT CAAAGGACAG 
CTGCTGCCCC CACCGCCTGT TCTCCCCGCC GCTGCTGTGG AGACCCCCGA TCACGCCGAG 
CTGCGCCTGG ACGCCCTGAC CCGTGACAGC AGCGGCAACT ACGAGTGCAG CGTCTCCAAC 
GACGTGGGCT CAGCCACCTG CCTCTTCCAG GTCTCCGCCA AAGCCTACAG CCCGGAGTTT 
TACTTCGACA CCCCCAACCC CACCCGCAGC CACAAGCTGT CCAAGAATTA CTCCTATGTG 
CTACAGTGGA CACAGAGAGA GCCCGATGCT GTGGACCCCG TGCTCAACTA CAGACTCAGC 
ATCCGACAGT TGAACCAGCA CAATGCCATG GTCAAGGCCA TCCCCGTTCG TCGTGTGGAG 
AAGGGACAGC TGCTGGAGTA TATCTTGACT GACCTCCGAG TACCCCACAG CTATGAGATC 
CGCCTCACAC CCTACACCAC CTTTGGGGCT GGTGACATGG CTTCCCGCAT CATCCACTAC 
ACAGAGCCTA TCAACTTGCC CAGCCTGTCA GACAACACCT GTCACTTTGA GGATGAGAAA 
ATCTGCGGCT ACACCCAGGA TCTGACAGAC AACTTTGACT GGACGCGGCA GAATGCTCTT 
ACCCAGAACC CCAAGCGCTC TCCTAATACT GGGCCCCCTA CTGACATCAG TGGCACCCCT 
GAAGGCTATT ACATGTTCAT TGAGACATCC AGACCCAGGG AGCTGGGGGA CCGTGCAAGG 
CTGGTGAGTC CCCTGTACAA CGCCAGTGCC AAGTTCTACT GTGTCTCTTT CTTCTACCAC 
ATGTATGGGA AACACATCGG CTCCCTCAAC CTTCTGGTGC GGTCCAGGAA CAAAGGCACT 
CTAGACACGC ATGCCTGGTC CCTCAGTGGC AATAAGGGTA ACGTGTGGCA GCAGGCCCAT 
GTACCCATTA ACCCCAGTGG GCCCTTCCAG ATTATTTTTG AGGGGGTTCG AGGCTCGGGC 
TACCTGGGGG ATATTGCCAT AGATGATGTC ACACTGAAGA AGGGAGAGTG TCCCCGGAGG 
CAGATGGATC CCAACAAAGT GGTTGTGATG CCAGGCAGCG GAGCCCCACG CCTGTCCAGC 
CTGCAACTTT GGGGATCCAT GGCCATCTTC CTCTTGGCGT TGCAAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdga1 ( XM_006525041.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525104.1 Mus musculus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu54118
Clone ID Related Accession (Same CDS sequence)	XM_006525041.3 , XM_006525041.4
Accession Version	XM_006525041.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	MAM domain-containing glycosylphosphatidylinositol anchor protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525041.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGA CCTGTCTTCT ACTTCTGGCG CTTATCCCCT TCCACTGTCG GGGACAAGGA 
GTCTATGCTC CAGCCCAGGC CCAGATTGTG CATGCGGGGC AGGCCTGTGT GGTGAAGGAG 
GATAACATCA GCGAGCGGGT CTACACCATC CGGGAGAGCG ACACCCTGGT GCTGCAGTGC 
CTGGTCACGG GACACCCTCG GCCCCAGGTG CGGTGGACCA AAACTGCCGG CAGCGCATCG 
GACAAGTTCC AGGAGACGTC GGTCTTCAAT GAGACTCTGC GGATCGAGCG CATCGCACGC 
ACGCAGGGAG GTCGCTACTA CTGCAAGGCG GAGAACGGAG TGGGCGTGCC GGCCATCAAG 
TCTATCCGCG TGGACGTGCA GTACTTGGAT GAGCCTGTGC TAACGGTGCA TCAGACAGTG 
AGCGATGTCC GAGGCAACTT CTACCAGGAG AAGACCGTGT TTCTACGCTG TACCGTCAGC 
TCCAACCCGC CCGCCCGCTT TATCTGGAAG CGGGGCTCCG ACACCCTGTC CCACAGCCAG 
GATAATGGGG TTGACATCTA CGAGCCTCTC TATACTCAGG GGGAGACGAA GGTCCTGAAG 
CTGAAGAACC TGCGGCCCCA AGACTATGCC AGCTACACCT GCCAGGTGTC CGTGCGCAAT 
GTGTGCGGCA TCCCAGACAA GGCTATCACC TTCCAGCTCA CCAACACCAC GGCACCTCCA 
GCCTTGAAGC TGTCTGTGAA CGAAACTCTG GTGGTGAACC CTGGGGAGAA CGTGACAGTG 
CAGTGTCTGC TGACGGGCGG CGATCCCCTC CCCCAGCTGC ACTGGTCCCA CGGACCTGGC 
CCACTGCCCC TGGGTGCTCT GGCTCAGGGT GGTACCCTCA GCATCCCTTC AGTGCAGGCC 
CGGGATTCTG GCTACTACAA CTGCACAGCC ACCAACAATG TAGGCAACCC AGCTAAGAAG 
ACCGTCAACC TGCTGGTGCG ATCCCTGAAG AACGCCACAT TCCAGATCAC CCCCGACATG 
ATCAAAGAAA GCGAGAACAT TCAACTCGGT CAGGACCTGA AGCTGTCATG CCATGTGGAT 
GCGGTGCCCC AGGAGAAGGT GAACTATCAG TGGTTTAAAA ACGGCAAGCC GGCACGCACA 
TCCAAGCGGC TGCTGGTGAC CCGAAATGAC CCCGAGTTGC CCGCTGTCAC CAGCAGCCTG 
GAGCTCATTG ACCTGCACTT CAGTGACTAC GGCACTTATC TGTGCATGGC TTCCTTCCCA 
GGGTCACCAG TGCCTGACCT CAGCATAGAG GTCAACATTT CCTCAGAGAC AGTACCACCC 
ACCATCAGTG TGCCCAAGGG CCGAGCAGTG GTGACAGTGA GGGAGGGCTC TCCTGCTGAG 
CTCCAGTGCG AAGTTCGTGG CAAGCCCCGG CCTCCAGTGC TATGGTCTCG GGTGGACAAG 
GAGGCCGCCC TTCTGCCCTC GGGGCTGGCC CTGGAGGAGA CTCCGGATGG GAAGCTGCGG 
CTGGAGAGTG TGAGCCGCGA CATGAGCGGC ACCTACCGCT GCCAGACAGC TCGCTACAAT 
GGCTTCAACG TACGACCCCG GGAGGCTCAG GTGCAGCTGA CAGTGCACTT TCCTCCAGAG 
GTGGAGCCCA GTTCCCAGGA CGTGCGCCAG GCGCTGGGCC GGCCTGTGCT CCTGCGCTGC 
TCGCTTCTGC GTGGCAGTCC TCAGCGCATC GCCTCGGCCG TGTGGCGCTT CAAAGGACAG 
CTGCTGCCCC CACCGCCTGT TCTCCCCGCC GCTGCTGTGG AGACCCCCGA TCACGCCGAG 
CTGCGCCTGG ACGCCCTGAC CCGTGACAGC AGCGGCAACT ACGAGTGCAG CGTCTCCAAC 
GACGTGGGCT CAGCCACCTG CCTCTTCCAG GTCTCCGCCA AAGCCTACAG CCCGGAGTTT 
TACTTCGACA CCCCCAACCC CACCCGCAGC CACAAGCTGT CCAAGAATTA CTCCTATGTG 
CTACAGTGGA CACAGAGAGA GCCCGATGCT GTGGACCCCG TGCTCAACTA CAGACTCAGC 
ATCCGACAGT TGAACCAGCA CAATGCCATG GTCAAGGCCA TCCCCGTTCG TCGTGTGGAG 
AAGGGACAGC TGCTGGAGTA TATCTTGACT GACCTCCGAG TACCCCACAG CTATGAGATC 
CGCCTCACAC CCTACACCAC CTTTGGGGCT GGTGACATGG CTTCCCGCAT CATCCACTAC 
ACAGAGCCTA TCAACTTGCC CAGCCTGTCA GACAACACCT GTCACTTTGA GGATGAGAAA 
ATCTGCGGCT ACACCCAGGA TCTGACAGAC AACTTTGACT GGACGCGGCA GAATGCTCTT 
ACCCAGAACC CCAAGCGCTC TCCTAATACT GGGCCCCCTA CTGACATCAG TGGCACCCCT 
GAAGGCTATT ACATGTTCAT TGAGACATCC AGACCCAGGG AGCTGGGGGA CCGTGCAAGG 
CTGGTGAGTC CCCTGTACAA CGCCAGTGCC AAGTTCTACT GTGTCTCTTT CTTCTACCAC 
ATGTATGGGA AACACATCGG CTCCCTCAAC CTTCTGGTGC GGTCCAGGAA CAAAGGCACT 
CTAGACACGC ATGCCTGGTC CCTCAGTGGC AATAAGGGTA ACGTGTGGCA GCAGGCCCAT 
GTACCCATTA ACCCCAGTGG GCCCTTCCAG ATGATGTCAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525104.1
CDS	913..3597
Translation	MEVTCLLLLALIPFHCRGQGVYAPAQAQIVHAGQACVVKEDNISERVYTIRESDTLVLQCLVTGHPRPQVRWTKTAGSASDKFQETSVFNETLRIERIARTQGGRYYCKAENGVGVPAIKSIRVDVQYLDEPVLTVHQTVSDVRGNFYQEKTVFLRCTVSSNPPARFIWKRGSDTLSHSQDNGVDIYEPLYTQGETKVLKLKNLRPQDYASYTCQVSVRNVCGIPDKAITFQLTNTTAPPALKLSVNETLVVNPGENVTVQCLLTGGDPLPQLHWSHGPGPLPLGALAQGGTLSIPSVQARDSGYYNCTATNNVGNPAKKTVNLLVRSLKNATFQITPDMIKESENIQLGQDLKLSCHVDAVPQEKVNYQWFKNGKPARTSKRLLVTRNDPELPAVTSSLELIDLHFSDYGTYLCMASFPGSPVPDLSIEVNISSETVPPTISVPKGRAVVTVREGSPAELQCEVRGKPRPPVLWSRVDKEAALLPSGLALEETPDGKLRLESVSRDMSGTYRCQTARYNGFNVRPREAQVQLTVHFPPEVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVLPAAAVETPDHAELRLDALTRDSSGNYECSVSNDVGSATCLFQVSAKAYSPEFYFDTPNPTRSHKLSKNYSYVLQWTQREPDAVDPVLNYRLSIRQLNQHNAMVKAIPVRRVEKGQLLEYILTDLRVPHSYEIRLTPYTTFGAGDMASRIIHYTEPINLPSLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPEGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGTLDTHAWSLSGNKGNVWQQAHVPINPSGPFQMMSH

Target ORF information:

RefSeq Version	XM_006525041.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525041.3

ORF Insert Sequence:

ATGGAGGTGA CCTGTCTTCT ACTTCTGGCG CTTATCCCCT TCCACTGTCG GGGACAAGGA 
GTCTATGCTC CAGCCCAGGC CCAGATTGTG CATGCGGGGC AGGCCTGTGT GGTGAAGGAG 
GATAACATCA GCGAGCGGGT CTACACCATC CGGGAGAGCG ACACCCTGGT GCTGCAGTGC 
CTGGTCACGG GACACCCTCG GCCCCAGGTG CGGTGGACCA AAACTGCCGG CAGCGCATCG 
GACAAGTTCC AGGAGACGTC GGTCTTCAAT GAGACTCTGC GGATCGAGCG CATCGCACGC 
ACGCAGGGAG GTCGCTACTA CTGCAAGGCG GAGAACGGAG TGGGCGTGCC GGCCATCAAG 
TCTATCCGCG TGGACGTGCA GTACTTGGAT GAGCCTGTGC TAACGGTGCA TCAGACAGTG 
AGCGATGTCC GAGGCAACTT CTACCAGGAG AAGACCGTGT TTCTACGCTG TACCGTCAGC 
TCCAACCCGC CCGCCCGCTT TATCTGGAAG CGGGGCTCCG ACACCCTGTC CCACAGCCAG 
GATAATGGGG TTGACATCTA CGAGCCTCTC TATACTCAGG GGGAGACGAA GGTCCTGAAG 
CTGAAGAACC TGCGGCCCCA AGACTATGCC AGCTACACCT GCCAGGTGTC CGTGCGCAAT 
GTGTGCGGCA TCCCAGACAA GGCTATCACC TTCCAGCTCA CCAACACCAC GGCACCTCCA 
GCCTTGAAGC TGTCTGTGAA CGAAACTCTG GTGGTGAACC CTGGGGAGAA CGTGACAGTG 
CAGTGTCTGC TGACGGGCGG CGATCCCCTC CCCCAGCTGC ACTGGTCCCA CGGACCTGGC 
CCACTGCCCC TGGGTGCTCT GGCTCAGGGT GGTACCCTCA GCATCCCTTC AGTGCAGGCC 
CGGGATTCTG GCTACTACAA CTGCACAGCC ACCAACAATG TAGGCAACCC AGCTAAGAAG 
ACCGTCAACC TGCTGGTGCG ATCCCTGAAG AACGCCACAT TCCAGATCAC CCCCGACATG 
ATCAAAGAAA GCGAGAACAT TCAACTCGGT CAGGACCTGA AGCTGTCATG CCATGTGGAT 
GCGGTGCCCC AGGAGAAGGT GAACTATCAG TGGTTTAAAA ACGGCAAGCC GGCACGCACA 
TCCAAGCGGC TGCTGGTGAC CCGAAATGAC CCCGAGTTGC CCGCTGTCAC CAGCAGCCTG 
GAGCTCATTG ACCTGCACTT CAGTGACTAC GGCACTTATC TGTGCATGGC TTCCTTCCCA 
GGGTCACCAG TGCCTGACCT CAGCATAGAG GTCAACATTT CCTCAGAGAC AGTACCACCC 
ACCATCAGTG TGCCCAAGGG CCGAGCAGTG GTGACAGTGA GGGAGGGCTC TCCTGCTGAG 
CTCCAGTGCG AAGTTCGTGG CAAGCCCCGG CCTCCAGTGC TATGGTCTCG GGTGGACAAG 
GAGGCCGCCC TTCTGCCCTC GGGGCTGGCC CTGGAGGAGA CTCCGGATGG GAAGCTGCGG 
CTGGAGAGTG TGAGCCGCGA CATGAGCGGC ACCTACCGCT GCCAGACAGC TCGCTACAAT 
GGCTTCAACG TACGACCCCG GGAGGCTCAG GTGCAGCTGA CAGTGCACTT TCCTCCAGAG 
GTGGAGCCCA GTTCCCAGGA CGTGCGCCAG GCGCTGGGCC GGCCTGTGCT CCTGCGCTGC 
TCGCTTCTGC GTGGCAGTCC TCAGCGCATC GCCTCGGCCG TGTGGCGCTT CAAAGGACAG 
CTGCTGCCCC CACCGCCTGT TCTCCCCGCC GCTGCTGTGG AGACCCCCGA TCACGCCGAG 
CTGCGCCTGG ACGCCCTGAC CCGTGACAGC AGCGGCAACT ACGAGTGCAG CGTCTCCAAC 
GACGTGGGCT CAGCCACCTG CCTCTTCCAG GTCTCCGCCA AAGCCTACAG CCCGGAGTTT 
TACTTCGACA CCCCCAACCC CACCCGCAGC CACAAGCTGT CCAAGAATTA CTCCTATGTG 
CTACAGTGGA CACAGAGAGA GCCCGATGCT GTGGACCCCG TGCTCAACTA CAGACTCAGC 
ATCCGACAGT TGAACCAGCA CAATGCCATG GTCAAGGCCA TCCCCGTTCG TCGTGTGGAG 
AAGGGACAGC TGCTGGAGTA TATCTTGACT GACCTCCGAG TACCCCACAG CTATGAGATC 
CGCCTCACAC CCTACACCAC CTTTGGGGCT GGTGACATGG CTTCCCGCAT CATCCACTAC 
ACAGAGCCTA TCAACTTGCC CAGCCTGTCA GACAACACCT GTCACTTTGA GGATGAGAAA 
ATCTGCGGCT ACACCCAGGA TCTGACAGAC AACTTTGACT GGACGCGGCA GAATGCTCTT 
ACCCAGAACC CCAAGCGCTC TCCTAATACT GGGCCCCCTA CTGACATCAG TGGCACCCCT 
GAAGGCTATT ACATGTTCAT TGAGACATCC AGACCCAGGG AGCTGGGGGA CCGTGCAAGG 
CTGGTGAGTC CCCTGTACAA CGCCAGTGCC AAGTTCTACT GTGTCTCTTT CTTCTACCAC 
ATGTATGGGA AACACATCGG CTCCCTCAAC CTTCTGGTGC GGTCCAGGAA CAAAGGCACT 
CTAGACACGC ATGCCTGGTC CCTCAGTGGC AATAAGGGTA ACGTGTGGCA GCAGGCCCAT 
GTACCCATTA ACCCCAGTGG GCCCTTCCAG ATGATGTCAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdga1 ( XM_006525041.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525104.1 Mus musculus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), transcript variant X1, mRNA.

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CloneID	OMu54118
Clone ID Related Accession (Same CDS sequence)	XM_006525041.3 , XM_006525041.4
Accession Version	XM_006525041.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAM domain-containing glycosylphosphatidylinositol anchor protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525041.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGA CCTGTCTTCT ACTTCTGGCG CTTATCCCCT TCCACTGTCG GGGACAAGGA 
GTCTATGCTC CAGCCCAGGC CCAGATTGTG CATGCGGGGC AGGCCTGTGT GGTGAAGGAG 
GATAACATCA GCGAGCGGGT CTACACCATC CGGGAGAGCG ACACCCTGGT GCTGCAGTGC 
CTGGTCACGG GACACCCTCG GCCCCAGGTG CGGTGGACCA AAACTGCCGG CAGCGCATCG 
GACAAGTTCC AGGAGACGTC GGTCTTCAAT GAGACTCTGC GGATCGAGCG CATCGCACGC 
ACGCAGGGAG GTCGCTACTA CTGCAAGGCG GAGAACGGAG TGGGCGTGCC GGCCATCAAG 
TCTATCCGCG TGGACGTGCA GTACTTGGAT GAGCCTGTGC TAACGGTGCA TCAGACAGTG 
AGCGATGTCC GAGGCAACTT CTACCAGGAG AAGACCGTGT TTCTACGCTG TACCGTCAGC 
TCCAACCCGC CCGCCCGCTT TATCTGGAAG CGGGGCTCCG ACACCCTGTC CCACAGCCAG 
GATAATGGGG TTGACATCTA CGAGCCTCTC TATACTCAGG GGGAGACGAA GGTCCTGAAG 
CTGAAGAACC TGCGGCCCCA AGACTATGCC AGCTACACCT GCCAGGTGTC CGTGCGCAAT 
GTGTGCGGCA TCCCAGACAA GGCTATCACC TTCCAGCTCA CCAACACCAC GGCACCTCCA 
GCCTTGAAGC TGTCTGTGAA CGAAACTCTG GTGGTGAACC CTGGGGAGAA CGTGACAGTG 
CAGTGTCTGC TGACGGGCGG CGATCCCCTC CCCCAGCTGC ACTGGTCCCA CGGACCTGGC 
CCACTGCCCC TGGGTGCTCT GGCTCAGGGT GGTACCCTCA GCATCCCTTC AGTGCAGGCC 
CGGGATTCTG GCTACTACAA CTGCACAGCC ACCAACAATG TAGGCAACCC AGCTAAGAAG 
ACCGTCAACC TGCTGGTGCG ATCCCTGAAG AACGCCACAT TCCAGATCAC CCCCGACATG 
ATCAAAGAAA GCGAGAACAT TCAACTCGGT CAGGACCTGA AGCTGTCATG CCATGTGGAT 
GCGGTGCCCC AGGAGAAGGT GAACTATCAG TGGTTTAAAA ACGGCAAGCC GGCACGCACA 
TCCAAGCGGC TGCTGGTGAC CCGAAATGAC CCCGAGTTGC CCGCTGTCAC CAGCAGCCTG 
GAGCTCATTG ACCTGCACTT CAGTGACTAC GGCACTTATC TGTGCATGGC TTCCTTCCCA 
GGGTCACCAG TGCCTGACCT CAGCATAGAG GTCAACATTT CCTCAGAGAC AGTACCACCC 
ACCATCAGTG TGCCCAAGGG CCGAGCAGTG GTGACAGTGA GGGAGGGCTC TCCTGCTGAG 
CTCCAGTGCG AAGTTCGTGG CAAGCCCCGG CCTCCAGTGC TATGGTCTCG GGTGGACAAG 
GAGGCCGCCC TTCTGCCCTC GGGGCTGGCC CTGGAGGAGA CTCCGGATGG GAAGCTGCGG 
CTGGAGAGTG TGAGCCGCGA CATGAGCGGC ACCTACCGCT GCCAGACAGC TCGCTACAAT 
GGCTTCAACG TACGACCCCG GGAGGCTCAG GTGCAGCTGA CAGTGCACTT TCCTCCAGAG 
GTGGAGCCCA GTTCCCAGGA CGTGCGCCAG GCGCTGGGCC GGCCTGTGCT CCTGCGCTGC 
TCGCTTCTGC GTGGCAGTCC TCAGCGCATC GCCTCGGCCG TGTGGCGCTT CAAAGGACAG 
CTGCTGCCCC CACCGCCTGT TCTCCCCGCC GCTGCTGTGG AGACCCCCGA TCACGCCGAG 
CTGCGCCTGG ACGCCCTGAC CCGTGACAGC AGCGGCAACT ACGAGTGCAG CGTCTCCAAC 
GACGTGGGCT CAGCCACCTG CCTCTTCCAG GTCTCCGCCA AAGCCTACAG CCCGGAGTTT 
TACTTCGACA CCCCCAACCC CACCCGCAGC CACAAGCTGT CCAAGAATTA CTCCTATGTG 
CTACAGTGGA CACAGAGAGA GCCCGATGCT GTGGACCCCG TGCTCAACTA CAGACTCAGC 
ATCCGACAGT TGAACCAGCA CAATGCCATG GTCAAGGCCA TCCCCGTTCG TCGTGTGGAG 
AAGGGACAGC TGCTGGAGTA TATCTTGACT GACCTCCGAG TACCCCACAG CTATGAGATC 
CGCCTCACAC CCTACACCAC CTTTGGGGCT GGTGACATGG CTTCCCGCAT CATCCACTAC 
ACAGAGCCTA TCAACTTGCC CAGCCTGTCA GACAACACCT GTCACTTTGA GGATGAGAAA 
ATCTGCGGCT ACACCCAGGA TCTGACAGAC AACTTTGACT GGACGCGGCA GAATGCTCTT 
ACCCAGAACC CCAAGCGCTC TCCTAATACT GGGCCCCCTA CTGACATCAG TGGCACCCCT 
GAAGGCTATT ACATGTTCAT TGAGACATCC AGACCCAGGG AGCTGGGGGA CCGTGCAAGG 
CTGGTGAGTC CCCTGTACAA CGCCAGTGCC AAGTTCTACT GTGTCTCTTT CTTCTACCAC 
ATGTATGGGA AACACATCGG CTCCCTCAAC CTTCTGGTGC GGTCCAGGAA CAAAGGCACT 
CTAGACACGC ATGCCTGGTC CCTCAGTGGC AATAAGGGTA ACGTGTGGCA GCAGGCCCAT 
GTACCCATTA ACCCCAGTGG GCCCTTCCAG ATGATGTCAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525104.1
CDS	1071..3755
Translation	MEVTCLLLLALIPFHCRGQGVYAPAQAQIVHAGQACVVKEDNISERVYTIRESDTLVLQCLVTGHPRPQVRWTKTAGSASDKFQETSVFNETLRIERIARTQGGRYYCKAENGVGVPAIKSIRVDVQYLDEPVLTVHQTVSDVRGNFYQEKTVFLRCTVSSNPPARFIWKRGSDTLSHSQDNGVDIYEPLYTQGETKVLKLKNLRPQDYASYTCQVSVRNVCGIPDKAITFQLTNTTAPPALKLSVNETLVVNPGENVTVQCLLTGGDPLPQLHWSHGPGPLPLGALAQGGTLSIPSVQARDSGYYNCTATNNVGNPAKKTVNLLVRSLKNATFQITPDMIKESENIQLGQDLKLSCHVDAVPQEKVNYQWFKNGKPARTSKRLLVTRNDPELPAVTSSLELIDLHFSDYGTYLCMASFPGSPVPDLSIEVNISSETVPPTISVPKGRAVVTVREGSPAELQCEVRGKPRPPVLWSRVDKEAALLPSGLALEETPDGKLRLESVSRDMSGTYRCQTARYNGFNVRPREAQVQLTVHFPPEVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVLPAAAVETPDHAELRLDALTRDSSGNYECSVSNDVGSATCLFQVSAKAYSPEFYFDTPNPTRSHKLSKNYSYVLQWTQREPDAVDPVLNYRLSIRQLNQHNAMVKAIPVRRVEKGQLLEYILTDLRVPHSYEIRLTPYTTFGAGDMASRIIHYTEPINLPSLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPEGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGTLDTHAWSLSGNKGNVWQQAHVPINPSGPFQMMSH

Target ORF information:

RefSeq Version	XM_006525041.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAM domain containing glycosylphosphatidylinositol anchor 1 (Mdga1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525041.4

ORF Insert Sequence:

ATGGAGGTGA CCTGTCTTCT ACTTCTGGCG CTTATCCCCT TCCACTGTCG GGGACAAGGA 
GTCTATGCTC CAGCCCAGGC CCAGATTGTG CATGCGGGGC AGGCCTGTGT GGTGAAGGAG 
GATAACATCA GCGAGCGGGT CTACACCATC CGGGAGAGCG ACACCCTGGT GCTGCAGTGC 
CTGGTCACGG GACACCCTCG GCCCCAGGTG CGGTGGACCA AAACTGCCGG CAGCGCATCG 
GACAAGTTCC AGGAGACGTC GGTCTTCAAT GAGACTCTGC GGATCGAGCG CATCGCACGC 
ACGCAGGGAG GTCGCTACTA CTGCAAGGCG GAGAACGGAG TGGGCGTGCC GGCCATCAAG 
TCTATCCGCG TGGACGTGCA GTACTTGGAT GAGCCTGTGC TAACGGTGCA TCAGACAGTG 
AGCGATGTCC GAGGCAACTT CTACCAGGAG AAGACCGTGT TTCTACGCTG TACCGTCAGC 
TCCAACCCGC CCGCCCGCTT TATCTGGAAG CGGGGCTCCG ACACCCTGTC CCACAGCCAG 
GATAATGGGG TTGACATCTA CGAGCCTCTC TATACTCAGG GGGAGACGAA GGTCCTGAAG 
CTGAAGAACC TGCGGCCCCA AGACTATGCC AGCTACACCT GCCAGGTGTC CGTGCGCAAT 
GTGTGCGGCA TCCCAGACAA GGCTATCACC TTCCAGCTCA CCAACACCAC GGCACCTCCA 
GCCTTGAAGC TGTCTGTGAA CGAAACTCTG GTGGTGAACC CTGGGGAGAA CGTGACAGTG 
CAGTGTCTGC TGACGGGCGG CGATCCCCTC CCCCAGCTGC ACTGGTCCCA CGGACCTGGC 
CCACTGCCCC TGGGTGCTCT GGCTCAGGGT GGTACCCTCA GCATCCCTTC AGTGCAGGCC 
CGGGATTCTG GCTACTACAA CTGCACAGCC ACCAACAATG TAGGCAACCC AGCTAAGAAG 
ACCGTCAACC TGCTGGTGCG ATCCCTGAAG AACGCCACAT TCCAGATCAC CCCCGACATG 
ATCAAAGAAA GCGAGAACAT TCAACTCGGT CAGGACCTGA AGCTGTCATG CCATGTGGAT 
GCGGTGCCCC AGGAGAAGGT GAACTATCAG TGGTTTAAAA ACGGCAAGCC GGCACGCACA 
TCCAAGCGGC TGCTGGTGAC CCGAAATGAC CCCGAGTTGC CCGCTGTCAC CAGCAGCCTG 
GAGCTCATTG ACCTGCACTT CAGTGACTAC GGCACTTATC TGTGCATGGC TTCCTTCCCA 
GGGTCACCAG TGCCTGACCT CAGCATAGAG GTCAACATTT CCTCAGAGAC AGTACCACCC 
ACCATCAGTG TGCCCAAGGG CCGAGCAGTG GTGACAGTGA GGGAGGGCTC TCCTGCTGAG 
CTCCAGTGCG AAGTTCGTGG CAAGCCCCGG CCTCCAGTGC TATGGTCTCG GGTGGACAAG 
GAGGCCGCCC TTCTGCCCTC GGGGCTGGCC CTGGAGGAGA CTCCGGATGG GAAGCTGCGG 
CTGGAGAGTG TGAGCCGCGA CATGAGCGGC ACCTACCGCT GCCAGACAGC TCGCTACAAT 
GGCTTCAACG TACGACCCCG GGAGGCTCAG GTGCAGCTGA CAGTGCACTT TCCTCCAGAG 
GTGGAGCCCA GTTCCCAGGA CGTGCGCCAG GCGCTGGGCC GGCCTGTGCT CCTGCGCTGC 
TCGCTTCTGC GTGGCAGTCC TCAGCGCATC GCCTCGGCCG TGTGGCGCTT CAAAGGACAG 
CTGCTGCCCC CACCGCCTGT TCTCCCCGCC GCTGCTGTGG AGACCCCCGA TCACGCCGAG 
CTGCGCCTGG ACGCCCTGAC CCGTGACAGC AGCGGCAACT ACGAGTGCAG CGTCTCCAAC 
GACGTGGGCT CAGCCACCTG CCTCTTCCAG GTCTCCGCCA AAGCCTACAG CCCGGAGTTT 
TACTTCGACA CCCCCAACCC CACCCGCAGC CACAAGCTGT CCAAGAATTA CTCCTATGTG 
CTACAGTGGA CACAGAGAGA GCCCGATGCT GTGGACCCCG TGCTCAACTA CAGACTCAGC 
ATCCGACAGT TGAACCAGCA CAATGCCATG GTCAAGGCCA TCCCCGTTCG TCGTGTGGAG 
AAGGGACAGC TGCTGGAGTA TATCTTGACT GACCTCCGAG TACCCCACAG CTATGAGATC 
CGCCTCACAC CCTACACCAC CTTTGGGGCT GGTGACATGG CTTCCCGCAT CATCCACTAC 
ACAGAGCCTA TCAACTTGCC CAGCCTGTCA GACAACACCT GTCACTTTGA GGATGAGAAA 
ATCTGCGGCT ACACCCAGGA TCTGACAGAC AACTTTGACT GGACGCGGCA GAATGCTCTT 
ACCCAGAACC CCAAGCGCTC TCCTAATACT GGGCCCCCTA CTGACATCAG TGGCACCCCT 
GAAGGCTATT ACATGTTCAT TGAGACATCC AGACCCAGGG AGCTGGGGGA CCGTGCAAGG 
CTGGTGAGTC CCCTGTACAA CGCCAGTGCC AAGTTCTACT GTGTCTCTTT CTTCTACCAC 
ATGTATGGGA AACACATCGG CTCCCTCAAC CTTCTGGTGC GGTCCAGGAA CAAAGGCACT 
CTAGACACGC ATGCCTGGTC CCTCAGTGGC AATAAGGGTA ACGTGTGGCA GCAGGCCCAT 
GTACCCATTA ACCCCAGTGG GCCCTTCCAG ATGATGTCAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of Synapse Repressor MDGA1 Enhances Perisomatic Inhibition, Confers Resistance to Network Excitation, and Impairs Cognitive Function.
Cell reports21(13)3637-3645(2017 Dec)
Connor SA,Ammendrup-Johnsen I,Kishimoto Y,Karimi Tari P,Cvetkovska V,Harada T,Ojima D,Yamamoto T,Wang YT,Craig AM

A dual-strategy expression screen for candidate connectivity labels in the developing thalamus.
PloS one12(5)e0177977(2017)
Bibollet-Bahena O,Okafuji T,Hokamp K,Tear G,Mitchell KJ

Ctip1 Controls Acquisition of Sensory Area Identity and Establishment of Sensory Input Fields in the Developing Neocortex.
Neuron90(2)261-77(2016 04)
Greig LC,Woodworth MB,Greppi C,Macklis JD

Single-cell-level spatial gene expression in the embryonic neural differentiation niche.
Genome research25(4)570-81(2015 Apr)
Huang Y,Yu X,Sun N,Qiao N,Cao Y,Boyd-Kirkup JD,Shen Q,Han JD

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

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Mdga1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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