gRNAs
The following Med23 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Med23 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa11569 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001277054.1 | |
| Accession Version | NM_001277054.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4113bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-23 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | mediator of RNA polymerase II transcription subunit 23 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC139610.2. On or before Mar 2, 2013 this sequence version replaced XM_220093.6, XM_002728640.2, XM_003748671.1, XM_001053256.3, XM_002725463.2, XM_003753148.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGTACCAA TGGAGACGCA ACTGCAGAGC ATTTTCGAGG AGGTGGTGAA AACAGAAATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001263983.1 |
| CDS | 81..4193 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001277054.1 |
1 | ATGGTACCAA TGGAGACGCA ACTGCAGAGC ATTTTCGAGG AGGTGGTGAA AACAGAAATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa22036 | |
| Clone ID Related Accession (Same CDS sequence) | XM_008758612.2 | |
| Accession Version | XM_008758612.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4131bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | mediator of RNA polymerase II transcription subunit 23 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008758612.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGTACCAA TGGAGACGCA ACTGCAGAGC ATTTTCGAGG AGGTGGTGAA AACAGAAATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_008756834.1 |
| CDS | 846..4976 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_008758612.2 |
1 | ATGGTACCAA TGGAGACGCA ACTGCAGAGC ATTTTCGAGG AGGTGGTGAA AACAGAAATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa46773 | |
| Clone ID Related Accession (Same CDS sequence) | XM_008758613.2 | |
| Accession Version | XM_008758613.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | mediator of RNA polymerase II transcription subunit 23 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008758613.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGTACCAA TGGAGACGCA ACTGCAGAGC ATTTTCGAGG AGGTGGTGAA AACAGAAATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_008756835.1 |
| CDS | 846..4862 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_008758613.2 |
1 | ATGGTACCAA TGGAGACGCA ACTGCAGAGC ATTTTCGAGG AGGTGGTGAA AACAGAAATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
T-cells null for the MED23 subunit of mediator express decreased levels of KLF2 and inefficiently populate the peripheral lymphoid organs. |
The Mediator complex and transcription regulation. |
ATAC and Mediator coactivators form a stable complex and regulate a set of non-coding RNA genes. |
Structural and functional organization of TRAP220, the TRAP/mediator subunit that is targeted by nuclear receptors. |
Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase. |
