med23 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	med23
Entrez Gene ID	445483
Full Name	mediator complex subunit 23
Synonyms	crsp3,si:ch211-272f15.2,zgc:91979
General protein information	Preferred Names mediator complex subunit 23 Names mediator of RNA polymerase II transcription subunit 23 CRSP complex subunit 3 cofactor required for Sp1 transcriptional activation subunit 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021468405.1	XP_021324080.1	mediator of RNA polymerase II transcription subunit 23 isoform X1
XM_005160334.3	XP_005160391.1	mediator of RNA polymerase II transcription subunit 23 isoform X3
NM_001003990.1	NP_001003990.1	mediator of RNA polymerase II transcription subunit 23
XM_005160335.3	XP_005160392.1	mediator of RNA polymerase II transcription subunit 23 isoform X2
XM_005160333.3	XP_005160390.1	mediator of RNA polymerase II transcription subunit 23 isoform X1
XM_005160335.4	XP_005160392.1	mediator of RNA polymerase II transcription subunit 23 isoform X2
XM_005160334.4	XP_005160391.1	mediator of RNA polymerase II transcription subunit 23 isoform X3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Med23	NP_001159888.1
Gallus gallus (chicken)	MED23	XP_424537.3
Caenorhabditis elegans (roundworm)	sur-2	NP_493575.2
Bos taurus (cattle)	MED23	NP_001192691.1
Homo sapiens (human)	MED23	NP_004821.2
Rattus norvegicus (Norway rat)	Med23	NP_001263983.1
Pan troglodytes (chimpanzee)	MED23	XP_527503.3
Canis lupus familiaris (dog)	MED23	XP_005627145.1
	MED23	XP_002803940.1
Danio rerio (zebrafish)	med23	NP_001003990.1
Drosophila melanogaster (fruit fly)	MED23	NP_001137741.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006046	XP_316095.4
Xenopus tropicalis (tropical clawed frog)	med23	XP_002936353.2

Gene Ontology
Bibliography

Related articles in PubMed

Mediator MED23 Links Pigmentation and DNA Repair through the Transcription Factor MITF.
Xia M, Chen K, Yao X, Xu Y, Yao J, Yan J, Shao Z, Wang G
Cell reports20(8)1794-1804(2017 Aug)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Mediator Med23 deficiency enhances neural differentiation of murine embryonic stem cells through modulating BMP signaling.
Zhu W, Yao X, Liang Y, Liang D, Song L, Jing N, Li J, Wang G
Development (Cambridge, England)142(3)465-76(2015 Feb)

Mediator MED23 plays opposing roles in directing smooth muscle cell and adipocyte differentiation.
Yin JW, Liang Y, Park JY, Chen D, Yao X, Xiao Q, Liu Z, Jiang B, Fu Y, Bao M, Huang Y, Liu Y, Yan J, Zhu MS, Yang Z, Gao P, Tian B, Li D, Wang G
Genes & development26(19)2192-205(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following med23 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the med23 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa37594	NM_001003990.1 Latest version!	Danio rerio mediator complex subunit 23 (med23), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa24589	XM_005160333.3 Latest version!	Danio rerio mediator complex subunit 23 (med23), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa24589	XM_021468405.1 Latest version!	Danio rerio mediator complex subunit 23 (med23), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa24590	XM_005160334.4 Latest version!	Danio rerio mediator complex subunit 23 (med23), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa24591	XM_005160335.4 Latest version!	Danio rerio mediator complex subunit 23 (med23), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa24591	XM_005160335.3	Danio rerio mediator complex subunit 23 (med23), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa24590	XM_005160334.3	Danio rerio mediator complex subunit 23 (med23), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

med23 ( NM_001003990.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003990.1 Danio rerio mediator complex subunit 23 (med23), mRNA.

Price & Availability↑

CloneID	ODa37594
Clone ID Related Accession (Same CDS sequence)	NM_001003990.1
Accession Version	NM_001003990.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Danio rerio(zebrafish)
Product	mediator of RNA polymerase II transcription subunit 23
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC080264.1. ##Evidence-Data-START## Transcript exon combination :: BC080264.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTTCATGG ACAGCCCTGA GGATGAGAAG ACCAAACTCA CAAGCTGTTT AGGAGCCTTT 
AGGCAGTTCT GGAGTACCCT GCCGCAGGAG TCTCATGAGC AGTGTGTGCA GTGGATCGTG 
CGCTTCATTC ATGGTCAGCA CAGCCCCAAA CGCATCGCCT TCCTCTACGA CTGTCTGGCC 
ATGGCGGTGG AGACCAGCCT GCTGCCACCC AGGATGGTGT GTCAGGCTCT CATCAGCTCC 
GACAGTCTGG AGTGGGAACG GACTCAACTC TGGGCTTTGA CCTTCAAACT CATCCGCAAA 
ATCATCGGAG GAGTCGACTA TAAGGGTGTG AGAGATCTAT TGAAGGCCGT GTTGGACAAG 
ATTCAGACCA TCCCCAACTT TGTGAGCTCA GCTGTGGTTC AGCAGCTGCT CGCGGCACGA 
GAGGTTGTGG AGTATATACT GGACCGCAAC GCCTGTCTGC TGCCGGCGTA CTTCGCTGTG 
ACTGAGATCC GGAAACTTTG TCCAGAGGGG GCGCTCTCAC ACTGGCTGTT GGGCAGCCTG 
ATATCTGACT TTGTCGACAG TTTCAGACCG ACGGCACGTA TAAACTCCAT ATGTGGGCGC 
TGCAGTCTTC TGCCTGTGGT CAATAATTCT GGGGCCATCT GTAACTCATG GAAGCTGGAC 
CCCACCACTC TGCGTTTCCC TCTGAGAGGA ATGCTGCCGT ATGATAAGGA CCTGTTTGAT 
CCGCAGACAG GGTTGCTGAG GTATGTGCTG GAGCAGCCGT ACTCCAGAGA CATGGTGTGC 
AACATGCTGG GCCTCAACAA ACAGCACAAG CAGAGGTGTC CGGTTCTGGA GGACCAGCTG 
GTGGATCTGG TGGTTTATGC CATGGAGCGC TCTGAGACTG AGGAGCAGTT TGATGACGGC 
GGCACCAGCC AGCTCCTGTG GCAGCATCTG TCCAGCCAGC TCATCTTCTT CGTCCTCTTC 
CAGTTTGCCA GCTTCCCTCA CATGGTGCTG TCGCTCCATC AGAAGCTGGC AGGTCGGGGT 
TTGATCAAGG GCAGGGATCA TCTGATGTGG GTTCTGCTGC AGTTCATCTC CGGCAGCATT 
CAGAAGAACG CTCTGGGAGA CTTTCTCCCC GTCATGAAGC TCTTCGACCT GCTGTACCCT 
GAGAAAGAGT GTATTCCAGT CCCGGACATC AACAAGCCCC AGTCCACACA CGCATTCGCC 
ATGACCTGCA TCTGGATTCA CCTCAACCGC AAAGCCCAGA ACGACAACTC CAAACTGCAG 
ATCCCCATCC CACACTCACT GAAGCTGCAC CATGAGTTTC TCCAGCAGAG TCTGCGCAAT 
AAGAGTTTGC CCATGACAGA CTATAAAATC GCTCTGCTGT GTAACGCCTA CTCCACCAAC 
TCCGAGTGCT TCACGCTGCC CATGGGCGTC CTGGTGGAGA CCATCTACGG CAATGGTAGC 
ATGCGCATCA CTCTGCCAGG CACCAATTGC ATGGCGTCCG GCTCTGTCAC GCCACTGCCC 
ATGAACCTGC TGGACTCCCT AACCGTCCAT GCTAAGATGA GTTTGATCCA CAGCATCGCC 
ACCCGTGTTA TTAAACTAGC CCACGCTAAG TCCAGTCTGG CTCTGGCTCC TGCGCTGGTG 
GAGACGTACA GCCGGCTGCT GGTCTACATG GAGATTGAGT CGCTCGGCAT CAAGGGATTC 
ATCAGTCAGC TTCTGCCGAA TGTGTTCAAG TCTCACGCGT GGGGGATTCT GCACACACTG 
CTGGAGATGT TCAGCTACCG CATGCACCAC ATTCAGCCTC ATTACCGCGT GCAGCTCCTG 
TCCCATCTGC ACTCGCTCGC CGCTGTCCCA CAGACCAATC AGAACCAGCT GCACCTCTGT 
GTTGAAAGCA CAGCTCTGCG CCTGATCACG GCTCTGGGTA GTTCCGAGGT TCAGCCTCAG 
TTCACACGGT TCCTCAACGA CCCAAAAACA GTGCTGTCGG CCGAGTCTGA GGAGCTAAAC 
CGAGCACTCA TCCTCACACT GGCCCGGGCC ACTCACGTCA CAGATTTCTT TACAGGCTCG 
GACTCGATTC AGGGCACTTG GTGTAAAGAC ATCCTGCAGA CCATCATGAA CTTCACACCT 
CATAACTGGG CCTCTCACAC TCTTAGCTGT TTCCCTGCTC CACTGCAGGC GTTCTTCAAG 
CAGAATAATG TCCCTCAGGA GAGCCGCTTC AACCTGAAGA AGAACGTGGA GGAGGAGTAC 
AGGAAGTGGA AGTCCATGAC CAATGAGAAC GACATCATCA CCCACTTCTC CATGCAGGGC 
TCGCCGCCGC TCTTCCTGTG TCTGCTGTGG AAGATGCTGC TGGAGACGGA CCACATCAAC 
CAAATCGGCT TCAGGGTTCT GGAGCGCATC GGGGCGCGAG CGCTGGTGGC TCATGTGCGC 
ACGTTTGCCG ACTTCCTGGT GTACGAGTTC TCCACATCCG CTGGCGGACA GCAGCTAAAC 
AAGTGCATCG AGATCCTGAA CGACATGGTG TGGAAGTACA ATATCGTGAC CCTGGATAGA 
CTCATCCTCT GCCTGGCGAT GAGGAGTCAC GAAGGGAACG AGGCTCAGGT CTGCTACTTC 
ATCATCCAGC TTCTCCTGCT CAAACCCAAC GACTTCCGCA ACCGCGTCAG TGACTTTGTG 
AAGGAGAACG CTCCAGAACA CTGGCTGCAG AGCGACTGGC ACACCAAACA CATGACCTAC 
CACAAGAAGT ATCCAGAGAA GCTGTATTTC GAGGGTCTGG CGGAGCAGGT CAATCCTCCT 
ATCCAGCTGC AGTCTCAGTA TCTGCCCATT TACTTCGGCA ACGTGTGTCT GCGCTTCCTC 
CCCGTGTTCG ACATCGTCAT CCACCGCTTC CTGGAGCTGC TGCCGGTCTC CAAATCCCTG 
GAGACGCTGC TCGATCATCT GGGAGGACTC TACAAGTTTC ATGATCGGCC GGTGACGTAC 
CTGTACAACA CGCTGCATTA TTATGAGAGG CACCTACGCG ACCGCACAAA CCTGAAGAGG 
AAGCTGGTGC ATGCCATCAT GAGCTCGCTG AAGGACAATC GAACACCCGG CTGGTGCCTT 
AGCGAGACCT ACCTGAAGTG CGGCATGAAC GCCCGTGAAG ATAATGTCTG GATCCCTGAC 
GACACCTACT ACTGCAAACT CATCGGACGC CTCGTGGATA CCATGGCAGG TAAATCTCCA 
GGCCCGTTCC CCAACTGTGA CTGGAGGTTT AACGAGTTCC CGAACCCGGC CGCTCATGCG 
CTGCACGTCA CCTGTGTGGA GCTGATGGCT CTGGCGGTCC CGGGGAAAGA CGTGGGCAAT 
GCTCTGCTTA ATGTGGTGCT CAAGAGTCAG CCGCTGGTGC CTCGAGAGAA CATCACGGCC 
TGGATGAACG CCATCGGACT GGTCATCACC GCTCTGCCTG AGCCCTACTG GATCGTCCTG 
CATGACCGTA TCGTGAGTGT GATCAGCAGT GCGGTGCTCA GCTCTGGGTC AGATTGGCTG 
GGTTACCCAT TCCAGCTTCT GGACTTCACC GCTTGCCATC AGTCCTACTC AGAGATGCAC 
TGCAGTTACG TTCTGGCTCT GGCTCACGCC GTGTGGCATC ACTCCAGCAT CGGCCAGCTC 
TCGCTCATAC CCAAGTTCCT GTCGGAGGTC CTGAAGCCCA TCGTGAAGAC TGAGTTCCAG 
CTGCTGTACG TTTATCATCT GGTGGGCCCG TTTCTGCAGC GCTTCCAGCA GGAGAGAACC 
AGATGCATGC TGGAGATTGG AGTGGCGTTT TATGAGATGC TTCAGGCTGT GGATCAGCAC 
AGCAACCACC TGGCCTACAT GGACCCCATC TGTGACTTCC TGTACCACAT CAAATACATG 
TTTACTGGGG ACAGTGTCAA AGACCAGGTG GAGCGCATCA TCTGTTCTCT GCGTCCAGCA 
ATGCGTCTTC GTCTACGCTT CATCACACAC ATCAGTAAGA TGGAGCCGGC GGCGGTGCCC 
GTGTCCAACA GCAGCAGCGT CCAGCAGACC AGCAGCGCCT CCTCACCCAC AGCACAGAGC 
ACTGCTGGAG CCGCCATACC ACTGTCTGTG ACACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003990.1
CDS	296..4354
Translation	MFMDSPEDEKTKLTSCLGAFRQFWSTLPQESHEQCVQWIVRFIHGQHSPKRIAFLYDCLAMAVETSLLPPRMVCQALISSDSLEWERTQLWALTFKLIRKIIGGVDYKGVRDLLKAVLDKIQTIPNFVSSAVVQQLLAAREVVEYILDRNACLLPAYFAVTEIRKLCPEGALSHWLLGSLISDFVDSFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPTTLRFPLRGMLPYDKDLFDPQTGLLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEQFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALGDFLPVMKLFDLLYPEKECIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLRNKSLPMTDYKIALLCNAYSTNSECFTLPMGVLVETIYGNGSMRITLPGTNCMASGSVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSLALAPALVETYSRLLVYMEIESLGIKGFISQLLPNVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHSLAAVPQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMNFTPHNWASHTLSCFPAPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMTNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGFRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENAPEHWLQSDWHTKHMTYHKKYPEKLYFEGLAEQVNPPIQLQSQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYERHLRDRTNLKRKLVHAIMSSLKDNRTPGWCLSETYLKCGMNAREDNVWIPDDTYYCKLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLVITALPEPYWIVLHDRIVSVISSAVLSSGSDWLGYPFQLLDFTACHQSYSEMHCSYVLALAHAVWHHSSIGQLSLIPKFLSEVLKPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMLEIGVAFYEMLQAVDQHSNHLAYMDPICDFLYHIKYMFTGDSVKDQVERIICSLRPAMRLRLRFITHISKMEPAAVPVSNSSSVQQTSSASSPTAQSTAGAAIPLSVTQ

Target ORF information:

RefSeq Version	NM_001003990.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator complex subunit 23 (med23), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003990.1

ORF Insert Sequence:

ATGTTCATGG ACAGCCCTGA GGATGAGAAG ACCAAACTCA CAAGCTGTTT AGGAGCCTTT 
AGGCAGTTCT GGAGTACCCT GCCGCAGGAG TCTCATGAGC AGTGTGTGCA GTGGATCGTG 
CGCTTCATTC ATGGTCAGCA CAGCCCCAAA CGCATCGCCT TCCTCTACGA CTGTCTGGCC 
ATGGCGGTGG AGACCAGCCT GCTGCCACCC AGGATGGTGT GTCAGGCTCT CATCAGCTCC 
GACAGTCTGG AGTGGGAACG GACTCAACTC TGGGCTTTGA CCTTCAAACT CATCCGCAAA 
ATCATCGGAG GAGTCGACTA TAAGGGTGTG AGAGATCTAT TGAAGGCCGT GTTGGACAAG 
ATTCAGACCA TCCCCAACTT TGTGAGCTCA GCTGTGGTTC AGCAGCTGCT CGCGGCACGA 
GAGGTTGTGG AGTATATACT GGACCGCAAC GCCTGTCTGC TGCCGGCGTA CTTCGCTGTG 
ACTGAGATCC GGAAACTTTG TCCAGAGGGG GCGCTCTCAC ACTGGCTGTT GGGCAGCCTG 
ATATCTGACT TTGTCGACAG TTTCAGACCG ACGGCACGTA TAAACTCCAT ATGTGGGCGC 
TGCAGTCTTC TGCCTGTGGT CAATAATTCT GGGGCCATCT GTAACTCATG GAAGCTGGAC 
CCCACCACTC TGCGTTTCCC TCTGAGAGGA ATGCTGCCGT ATGATAAGGA CCTGTTTGAT 
CCGCAGACAG GGTTGCTGAG GTATGTGCTG GAGCAGCCGT ACTCCAGAGA CATGGTGTGC 
AACATGCTGG GCCTCAACAA ACAGCACAAG CAGAGGTGTC CGGTTCTGGA GGACCAGCTG 
GTGGATCTGG TGGTTTATGC CATGGAGCGC TCTGAGACTG AGGAGCAGTT TGATGACGGC 
GGCACCAGCC AGCTCCTGTG GCAGCATCTG TCCAGCCAGC TCATCTTCTT CGTCCTCTTC 
CAGTTTGCCA GCTTCCCTCA CATGGTGCTG TCGCTCCATC AGAAGCTGGC AGGTCGGGGT 
TTGATCAAGG GCAGGGATCA TCTGATGTGG GTTCTGCTGC AGTTCATCTC CGGCAGCATT 
CAGAAGAACG CTCTGGGAGA CTTTCTCCCC GTCATGAAGC TCTTCGACCT GCTGTACCCT 
GAGAAAGAGT GTATTCCAGT CCCGGACATC AACAAGCCCC AGTCCACACA CGCATTCGCC 
ATGACCTGCA TCTGGATTCA CCTCAACCGC AAAGCCCAGA ACGACAACTC CAAACTGCAG 
ATCCCCATCC CACACTCACT GAAGCTGCAC CATGAGTTTC TCCAGCAGAG TCTGCGCAAT 
AAGAGTTTGC CCATGACAGA CTATAAAATC GCTCTGCTGT GTAACGCCTA CTCCACCAAC 
TCCGAGTGCT TCACGCTGCC CATGGGCGTC CTGGTGGAGA CCATCTACGG CAATGGTAGC 
ATGCGCATCA CTCTGCCAGG CACCAATTGC ATGGCGTCCG GCTCTGTCAC GCCACTGCCC 
ATGAACCTGC TGGACTCCCT AACCGTCCAT GCTAAGATGA GTTTGATCCA CAGCATCGCC 
ACCCGTGTTA TTAAACTAGC CCACGCTAAG TCCAGTCTGG CTCTGGCTCC TGCGCTGGTG 
GAGACGTACA GCCGGCTGCT GGTCTACATG GAGATTGAGT CGCTCGGCAT CAAGGGATTC 
ATCAGTCAGC TTCTGCCGAA TGTGTTCAAG TCTCACGCGT GGGGGATTCT GCACACACTG 
CTGGAGATGT TCAGCTACCG CATGCACCAC ATTCAGCCTC ATTACCGCGT GCAGCTCCTG 
TCCCATCTGC ACTCGCTCGC CGCTGTCCCA CAGACCAATC AGAACCAGCT GCACCTCTGT 
GTTGAAAGCA CAGCTCTGCG CCTGATCACG GCTCTGGGTA GTTCCGAGGT TCAGCCTCAG 
TTCACACGGT TCCTCAACGA CCCAAAAACA GTGCTGTCGG CCGAGTCTGA GGAGCTAAAC 
CGAGCACTCA TCCTCACACT GGCCCGGGCC ACTCACGTCA CAGATTTCTT TACAGGCTCG 
GACTCGATTC AGGGCACTTG GTGTAAAGAC ATCCTGCAGA CCATCATGAA CTTCACACCT 
CATAACTGGG CCTCTCACAC TCTTAGCTGT TTCCCTGCTC CACTGCAGGC GTTCTTCAAG 
CAGAATAATG TCCCTCAGGA GAGCCGCTTC AACCTGAAGA AGAACGTGGA GGAGGAGTAC 
AGGAAGTGGA AGTCCATGAC CAATGAGAAC GACATCATCA CCCACTTCTC CATGCAGGGC 
TCGCCGCCGC TCTTCCTGTG TCTGCTGTGG AAGATGCTGC TGGAGACGGA CCACATCAAC 
CAAATCGGCT TCAGGGTTCT GGAGCGCATC GGGGCGCGAG CGCTGGTGGC TCATGTGCGC 
ACGTTTGCCG ACTTCCTGGT GTACGAGTTC TCCACATCCG CTGGCGGACA GCAGCTAAAC 
AAGTGCATCG AGATCCTGAA CGACATGGTG TGGAAGTACA ATATCGTGAC CCTGGATAGA 
CTCATCCTCT GCCTGGCGAT GAGGAGTCAC GAAGGGAACG AGGCTCAGGT CTGCTACTTC 
ATCATCCAGC TTCTCCTGCT CAAACCCAAC GACTTCCGCA ACCGCGTCAG TGACTTTGTG 
AAGGAGAACG CTCCAGAACA CTGGCTGCAG AGCGACTGGC ACACCAAACA CATGACCTAC 
CACAAGAAGT ATCCAGAGAA GCTGTATTTC GAGGGTCTGG CGGAGCAGGT CAATCCTCCT 
ATCCAGCTGC AGTCTCAGTA TCTGCCCATT TACTTCGGCA ACGTGTGTCT GCGCTTCCTC 
CCCGTGTTCG ACATCGTCAT CCACCGCTTC CTGGAGCTGC TGCCGGTCTC CAAATCCCTG 
GAGACGCTGC TCGATCATCT GGGAGGACTC TACAAGTTTC ATGATCGGCC GGTGACGTAC 
CTGTACAACA CGCTGCATTA TTATGAGAGG CACCTACGCG ACCGCACAAA CCTGAAGAGG 
AAGCTGGTGC ATGCCATCAT GAGCTCGCTG AAGGACAATC GAACACCCGG CTGGTGCCTT 
AGCGAGACCT ACCTGAAGTG CGGCATGAAC GCCCGTGAAG ATAATGTCTG GATCCCTGAC 
GACACCTACT ACTGCAAACT CATCGGACGC CTCGTGGATA CCATGGCAGG TAAATCTCCA 
GGCCCGTTCC CCAACTGTGA CTGGAGGTTT AACGAGTTCC CGAACCCGGC CGCTCATGCG 
CTGCACGTCA CCTGTGTGGA GCTGATGGCT CTGGCGGTCC CGGGGAAAGA CGTGGGCAAT 
GCTCTGCTTA ATGTGGTGCT CAAGAGTCAG CCGCTGGTGC CTCGAGAGAA CATCACGGCC 
TGGATGAACG CCATCGGACT GGTCATCACC GCTCTGCCTG AGCCCTACTG GATCGTCCTG 
CATGACCGTA TCGTGAGTGT GATCAGCAGT GCGGTGCTCA GCTCTGGGTC AGATTGGCTG 
GGTTACCCAT TCCAGCTTCT GGACTTCACC GCTTGCCATC AGTCCTACTC AGAGATGCAC 
TGCAGTTACG TTCTGGCTCT GGCTCACGCC GTGTGGCATC ACTCCAGCAT CGGCCAGCTC 
TCGCTCATAC CCAAGTTCCT GTCGGAGGTC CTGAAGCCCA TCGTGAAGAC TGAGTTCCAG 
CTGCTGTACG TTTATCATCT GGTGGGCCCG TTTCTGCAGC GCTTCCAGCA GGAGAGAACC 
AGATGCATGC TGGAGATTGG AGTGGCGTTT TATGAGATGC TTCAGGCTGT GGATCAGCAC 
AGCAACCACC TGGCCTACAT GGACCCCATC TGTGACTTCC TGTACCACAT CAAATACATG 
TTTACTGGGG ACAGTGTCAA AGACCAGGTG GAGCGCATCA TCTGTTCTCT GCGTCCAGCA 
ATGCGTCTTC GTCTACGCTT CATCACACAC ATCAGTAAGA TGGAGCCGGC GGCGGTGCCC 
GTGTCCAACA GCAGCAGCGT CCAGCAGACC AGCAGCGCCT CCTCACCCAC AGCACAGAGC 
ACTGCTGGAG CCGCCATACC ACTGTCTGTG ACACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med23 ( XM_021468405.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324080.1 Danio rerio mediator complex subunit 23 (med23), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24589
Clone ID Related Accession (Same CDS sequence)	XM_021468405.1 , XM_005160333.3
Accession Version	XM_021468405.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mediator of RNA polymerase II transcription subunit 23 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACA CACACTTCTC ATCCATTCAT CACAGTAACA ACAACAGCAG CAGTCACATC 
TCAATCATTG AGAAAACGGA GGTGATCGAG GAGGCGTTTG CTGGCATGTT CATGGACAGC 
CCTGAGGATG AGAAGACCAA ACTCACAAGC TGTTTAGGAG CCTTTAGGCA GTTCTGGAGT 
ACCCTGCCGC AGGAGTCTCA TGAGCAGTGT GTGCAGTGGA TCGTGCGCTT CATTCATGGT 
CAGCACAGCC CCAAACGCAT CGCCTTCCTC TACGACTGTC TGGCCATGGC GGTGGAGACC 
AGCCTGCTGC CACCCAGGAT GGTGTGTCAG GCTCTCATCA GCTCCGACAG TCTGGAGTGG 
GAACGGACTC AACTCTGGGC TTTGACCTTC AAACTCATCC GCAAAATCAT CGGAGGAGTC 
GACTATAAGG GTGTGAGAGA TCTATTGAAG GCCGTGTTGG ACAAGATTCA GACCATCCCC 
AACTTTGTGA GCTCAGCTGT GGTTCAGCAG CTGCTCGCGG CACGAGAGGT TGTGGAGTAT 
ATACTGGACC GCAACGCCTG TCTGCTGCCG GCGTACTTCG CTGTGACTGA GATCCGGAAA 
CTTTGTCCAG AGGGGGCGCT CTCACACTGG CTGTTGGGCA GCCTGATATC TGACTTTGTC 
GACAGTTTCA GACCGACGGC ACGTATAAAC TCCATATGTG GGCGCTGCAG TCTTCTGCCT 
GTGGTCAATA ATTCTGGGGC CATCTGTAAC TCATGGAAGC TGGACCCCAC CACTCTGCGT 
TTCCCTCTGA GAGGAATGCT GCCGTATGAT AAGGACCTGT TTGATCCGCA GACAGGGTTG 
CTGAGGTATG TGCTGGAGCA GCCGTACTCC AGAGACATGG TGTGCAACAT GCTGGGCCTC 
AACAAACAGC ACAAGCAGAG GTGTCCGGTT CTGGAGGACC AGCTGGTGGA TCTGGTGGTT 
TATGCCATGG AGCGCTCTGA GACTGAGGAG CAGTTTGATG ACGGCGGCAC CAGCCAGCTC 
CTGTGGCAGC ATCTGTCCAG CCAGCTCATC TTCTTCGTCC TCTTCCAGTT TGCCAGCTTC 
CCTCACATGG TGCTGTCGCT CCATCAGAAG CTGGCAGGTC GGGGTTTGAT CAAGGGCAGG 
GATCATCTGA TGTGGGTTCT GCTGCAGTTC ATCTCCGGCA GCATTCAGAA GAACGCTCTG 
GGAGACTTTC TCCCCGTCAT GAAGCTCTTC GACCTGCTGT ACCCTGAGAA AGAGTGTATT 
CCAGTCCCGG ACATCAACAA GCCCCAGTCC ACACACGCAT TCGCCATGAC CTGCATCTGG 
ATTCACCTCA ACCGCAAAGC CCAGAACGAC AACTCCAAAC TGCAGATCCC CATCCCACAC 
TCACTGAAGC TGCACCATGA GTTTCTCCAG CAGAGTCTGC GCAATAAGAG TTTGCCCATG 
ACAGACTATA AAATCGCTCT GCTGTGTAAC GCCTACTCCA CCAACTCCGA GTGCTTCACG 
CTGCCCATGG GCGTCCTGGT GGAGACCATC TACGGCAATG GTAGCATGCG CATCACTCTG 
CCAGGCACCA ATTGCATGGC GTCCGGCTCT GTCACGCCAC TGCCCATGAA CCTGCTGGAC 
TCCCTAACCG TCCATGCTAA GATGAGTTTG ATCCACAGCA TCGCCACCCG TGTTATTAAA 
CTAGCCCACG CTAAGTCCAG TCTGGCTCTG GCTCCTGCGC TGGTGGAGAC GTACAGCCGG 
CTGCTGGTCT ACATGGAGAT TGAGTCGCTC GGCATCAAGG GATTCATCAG TCAGCTTCTG 
CCGAATGTGT TCAAGTCTCA CGCGTGGGGG ATTCTGCACA CACTGCTGGA GATGTTCAGC 
TACCGCATGC ACCACATTCA GCCTCATTAC CGCGTGCAGC TCCTGTCCCA TCTGCACTCG 
CTCGCCGCTG TCCCACAGAC CAATCAGAAC CAGCTGCACC TCTGTGTTGA AAGCACAGCT 
CTGCGCCTGA TCACGGCTCT GGGTAGTTCC GAGGTTCAGC CTCAGTTCAC ACGGTTCCTC 
AACGACCCAA AAACAGTGCT GTCGGCCGAG TCTGAGGAGC TAAACCGAGC ACTCATCCTC 
ACACTGGCCC GGGCCACTCA CGTCACAGAT TTCTTTACAG GCTCGGACTC GATTCAGGGC 
ACTTGGTGTA AAGACATCCT GCAGACCATC ATGAACTTCA CACCTCATAA CTGGGCCTCT 
CACACTCTTA GCTGTTTCCC TGCTCCACTG CAGGCGTTCT TCAAGCAGAA TAATGTCCCT 
CAGGAGAGCC GCTTCAACCT GAAGAAGAAC GTGGAGGAGG AGTACAGGAA GTGGAAGTCC 
ATGACCAATG AGAACGACAT CATCACCCAC TTCTCCATGC AGGGCTCGCC GCCGCTCTTC 
CTGTGTCTGC TGTGGAAGAT GCTGCTGGAG ACGGACCACA TCAACCAAAT CGGCTTCAGG 
GTTCTGGAGC GCATCGGGGC GCGAGCGCTG GTGGCTCATG TGCGCACGTT TGCCGACTTC 
CTGGTGTACG AGTTCTCCAC ATCCGCTGGC GGACAGCAGC TAAACAAGTG CATCGAGATC 
CTGAACGACA TGGTGTGGAA GTACAATATC GTGACCCTGG ATAGACTCAT CCTCTGCCTG 
GCGATGAGGA GTCACGAAGG GAACGAGGCT CAGGTCTGCT ACTTCATCAT CCAGCTTCTC 
CTGCTCAAAC CCAACGACTT CCGCAACCGC GTCAGTGACT TTGTGAAGGA GAACGCTCCA 
GAACACTGGC TGCAGAGCGA CTGGCACACC AAACACATGA CCTACCACAA GAAGTATCCA 
GAGAAGCTGT ATTTCGAGGG TCTGGCGGAG CAGGTCAATC CTCCTATCCA GCTGCAGTCT 
CAGTATCTGC CCATTTACTT CGGCAACGTG TGTCTGCGCT TCCTCCCCGT GTTCGACATC 
GTCATCCACC GCTTCCTGGA GCTGCTGCCG GTCTCCAAAT CCCTGGAGAC GCTGCTCGAT 
CATCTGGGAG GACTCTACAA GTTTCATGAT CGGCCGGTGA CGTACCTGTA CAACACGCTG 
CATTATTATG AGAGGCACCT ACGCGACCGC ACAAACCTGA AGAGGAAGCT GGTGCATGCC 
ATCATGAGCT CGCTGAAGGA CAATCGAACA CCCGGCTGGT GCCTTAGCGA GACCTACCTG 
AAGTGCGGCA TGAACGCCCG TGAAGATAAT GTCTGGATCC CTGACGACAC CTACTACTGC 
AAACTCATCG GACGCCTCGT GGATACCATG GCAGGTAAAT CTCCAGGCCC GTTCCCCAAC 
TGTGACTGGA GGTTTAACGA GTTCCCGAAC CCGGCCGCTC ATGCGCTGCA CGTCACCTGT 
GTGGAGCTGA TGGCTCTGGC GGTCCCGGGG AAAGACGTGG GCAATGCTCT GCTTAATGTG 
GTGCTCAAGA GTCAGCCGCT GGTGCCTCGA GAGAACATCA CGGCCTGGAT GAACGCCATC 
GGACTGGTCA TCACCGCTCT GCCTGAGCCC TACTGGATCG TCCTGCATGA CCGTATCGTG 
AGTGTGATCA GCAGTGCGGT GCTCAGCTCT GGGTCAGATT GGCTGGGTTA CCCATTCCAG 
CTTCTGGACT TCACCGCTTG CCATCAGTCC TACTCAGAGA TGCACTGCAG TTACGTTCTG 
GCTCTGGCTC ACGCCGTGTG GCATCACTCC AGCATCGGCC AGCTCTCGCT CATACCCAAG 
TTCCTGTCGG AGGTCCTGAA GCCCATCGTG AAGACTGAGT TCCAGCTGCT GTACGTTTAT 
CATCTGGTGG GCCCGTTTCT GCAGCGCTTC CAGCAGGAGA GAACCAGATG CATGCTGGAG 
ATTGGAGTGG CGTTTTATGA GATGCTTCAG GCTGTGGATC AGCACAGCAA CCACCTGGCC 
TACATGGACC CCATCTGTGA CTTCCTGTAC CACATCAAAT ACATGTTTAC TGGGGACAGT 
GTCAAAGACC AGGTGGAGCG CATCATCTGT TCTCTGCGTC CAGCAATGCG TCTTCGTCTA 
CGCTTCATCA CACACATCAG TAAGATGGAG CCGGCGGCGG TGCCCGTGTC CAACAGCAGC 
AGCGTCCAGC AGACCAGCAG CGCCTCCTCA CCCACAGCAC AGAGCACTGC TGGAGCCGCC 
ATACCACTGT CTGTGACACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324080.1
CDS	191..4354
Translation	MQHTHFSSIHHSNNNSSSHISIIEKTEVIEEAFAGMFMDSPEDEKTKLTSCLGAFRQFWSTLPQESHEQCVQWIVRFIHGQHSPKRIAFLYDCLAMAVETSLLPPRMVCQALISSDSLEWERTQLWALTFKLIRKIIGGVDYKGVRDLLKAVLDKIQTIPNFVSSAVVQQLLAAREVVEYILDRNACLLPAYFAVTEIRKLCPEGALSHWLLGSLISDFVDSFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPTTLRFPLRGMLPYDKDLFDPQTGLLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEQFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALGDFLPVMKLFDLLYPEKECIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLRNKSLPMTDYKIALLCNAYSTNSECFTLPMGVLVETIYGNGSMRITLPGTNCMASGSVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSLALAPALVETYSRLLVYMEIESLGIKGFISQLLPNVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHSLAAVPQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMNFTPHNWASHTLSCFPAPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMTNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGFRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENAPEHWLQSDWHTKHMTYHKKYPEKLYFEGLAEQVNPPIQLQSQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYERHLRDRTNLKRKLVHAIMSSLKDNRTPGWCLSETYLKCGMNAREDNVWIPDDTYYCKLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLVITALPEPYWIVLHDRIVSVISSAVLSSGSDWLGYPFQLLDFTACHQSYSEMHCSYVLALAHAVWHHSSIGQLSLIPKFLSEVLKPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMLEIGVAFYEMLQAVDQHSNHLAYMDPICDFLYHIKYMFTGDSVKDQVERIICSLRPAMRLRLRFITHISKMEPAAVPVSNSSSVQQTSSASSPTAQSTAGAAIPLSVTQ

Target ORF information:

RefSeq Version	XM_021468405.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator complex subunit 23 (med23), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468405.1

ORF Insert Sequence:

ATGCAGCACA CACACTTCTC ATCCATTCAT CACAGTAACA ACAACAGCAG CAGTCACATC 
TCAATCATTG AGAAAACGGA GGTGATCGAG GAGGCGTTTG CTGGCATGTT CATGGACAGC 
CCTGAGGATG AGAAGACCAA ACTCACAAGC TGTTTAGGAG CCTTTAGGCA GTTCTGGAGT 
ACCCTGCCGC AGGAGTCTCA TGAGCAGTGT GTGCAGTGGA TCGTGCGCTT CATTCATGGT 
CAGCACAGCC CCAAACGCAT CGCCTTCCTC TACGACTGTC TGGCCATGGC GGTGGAGACC 
AGCCTGCTGC CACCCAGGAT GGTGTGTCAG GCTCTCATCA GCTCCGACAG TCTGGAGTGG 
GAACGGACTC AACTCTGGGC TTTGACCTTC AAACTCATCC GCAAAATCAT CGGAGGAGTC 
GACTATAAGG GTGTGAGAGA TCTATTGAAG GCCGTGTTGG ACAAGATTCA GACCATCCCC 
AACTTTGTGA GCTCAGCTGT GGTTCAGCAG CTGCTCGCGG CACGAGAGGT TGTGGAGTAT 
ATACTGGACC GCAACGCCTG TCTGCTGCCG GCGTACTTCG CTGTGACTGA GATCCGGAAA 
CTTTGTCCAG AGGGGGCGCT CTCACACTGG CTGTTGGGCA GCCTGATATC TGACTTTGTC 
GACAGTTTCA GACCGACGGC ACGTATAAAC TCCATATGTG GGCGCTGCAG TCTTCTGCCT 
GTGGTCAATA ATTCTGGGGC CATCTGTAAC TCATGGAAGC TGGACCCCAC CACTCTGCGT 
TTCCCTCTGA GAGGAATGCT GCCGTATGAT AAGGACCTGT TTGATCCGCA GACAGGGTTG 
CTGAGGTATG TGCTGGAGCA GCCGTACTCC AGAGACATGG TGTGCAACAT GCTGGGCCTC 
AACAAACAGC ACAAGCAGAG GTGTCCGGTT CTGGAGGACC AGCTGGTGGA TCTGGTGGTT 
TATGCCATGG AGCGCTCTGA GACTGAGGAG CAGTTTGATG ACGGCGGCAC CAGCCAGCTC 
CTGTGGCAGC ATCTGTCCAG CCAGCTCATC TTCTTCGTCC TCTTCCAGTT TGCCAGCTTC 
CCTCACATGG TGCTGTCGCT CCATCAGAAG CTGGCAGGTC GGGGTTTGAT CAAGGGCAGG 
GATCATCTGA TGTGGGTTCT GCTGCAGTTC ATCTCCGGCA GCATTCAGAA GAACGCTCTG 
GGAGACTTTC TCCCCGTCAT GAAGCTCTTC GACCTGCTGT ACCCTGAGAA AGAGTGTATT 
CCAGTCCCGG ACATCAACAA GCCCCAGTCC ACACACGCAT TCGCCATGAC CTGCATCTGG 
ATTCACCTCA ACCGCAAAGC CCAGAACGAC AACTCCAAAC TGCAGATCCC CATCCCACAC 
TCACTGAAGC TGCACCATGA GTTTCTCCAG CAGAGTCTGC GCAATAAGAG TTTGCCCATG 
ACAGACTATA AAATCGCTCT GCTGTGTAAC GCCTACTCCA CCAACTCCGA GTGCTTCACG 
CTGCCCATGG GCGTCCTGGT GGAGACCATC TACGGCAATG GTAGCATGCG CATCACTCTG 
CCAGGCACCA ATTGCATGGC GTCCGGCTCT GTCACGCCAC TGCCCATGAA CCTGCTGGAC 
TCCCTAACCG TCCATGCTAA GATGAGTTTG ATCCACAGCA TCGCCACCCG TGTTATTAAA 
CTAGCCCACG CTAAGTCCAG TCTGGCTCTG GCTCCTGCGC TGGTGGAGAC GTACAGCCGG 
CTGCTGGTCT ACATGGAGAT TGAGTCGCTC GGCATCAAGG GATTCATCAG TCAGCTTCTG 
CCGAATGTGT TCAAGTCTCA CGCGTGGGGG ATTCTGCACA CACTGCTGGA GATGTTCAGC 
TACCGCATGC ACCACATTCA GCCTCATTAC CGCGTGCAGC TCCTGTCCCA TCTGCACTCG 
CTCGCCGCTG TCCCACAGAC CAATCAGAAC CAGCTGCACC TCTGTGTTGA AAGCACAGCT 
CTGCGCCTGA TCACGGCTCT GGGTAGTTCC GAGGTTCAGC CTCAGTTCAC ACGGTTCCTC 
AACGACCCAA AAACAGTGCT GTCGGCCGAG TCTGAGGAGC TAAACCGAGC ACTCATCCTC 
ACACTGGCCC GGGCCACTCA CGTCACAGAT TTCTTTACAG GCTCGGACTC GATTCAGGGC 
ACTTGGTGTA AAGACATCCT GCAGACCATC ATGAACTTCA CACCTCATAA CTGGGCCTCT 
CACACTCTTA GCTGTTTCCC TGCTCCACTG CAGGCGTTCT TCAAGCAGAA TAATGTCCCT 
CAGGAGAGCC GCTTCAACCT GAAGAAGAAC GTGGAGGAGG AGTACAGGAA GTGGAAGTCC 
ATGACCAATG AGAACGACAT CATCACCCAC TTCTCCATGC AGGGCTCGCC GCCGCTCTTC 
CTGTGTCTGC TGTGGAAGAT GCTGCTGGAG ACGGACCACA TCAACCAAAT CGGCTTCAGG 
GTTCTGGAGC GCATCGGGGC GCGAGCGCTG GTGGCTCATG TGCGCACGTT TGCCGACTTC 
CTGGTGTACG AGTTCTCCAC ATCCGCTGGC GGACAGCAGC TAAACAAGTG CATCGAGATC 
CTGAACGACA TGGTGTGGAA GTACAATATC GTGACCCTGG ATAGACTCAT CCTCTGCCTG 
GCGATGAGGA GTCACGAAGG GAACGAGGCT CAGGTCTGCT ACTTCATCAT CCAGCTTCTC 
CTGCTCAAAC CCAACGACTT CCGCAACCGC GTCAGTGACT TTGTGAAGGA GAACGCTCCA 
GAACACTGGC TGCAGAGCGA CTGGCACACC AAACACATGA CCTACCACAA GAAGTATCCA 
GAGAAGCTGT ATTTCGAGGG TCTGGCGGAG CAGGTCAATC CTCCTATCCA GCTGCAGTCT 
CAGTATCTGC CCATTTACTT CGGCAACGTG TGTCTGCGCT TCCTCCCCGT GTTCGACATC 
GTCATCCACC GCTTCCTGGA GCTGCTGCCG GTCTCCAAAT CCCTGGAGAC GCTGCTCGAT 
CATCTGGGAG GACTCTACAA GTTTCATGAT CGGCCGGTGA CGTACCTGTA CAACACGCTG 
CATTATTATG AGAGGCACCT ACGCGACCGC ACAAACCTGA AGAGGAAGCT GGTGCATGCC 
ATCATGAGCT CGCTGAAGGA CAATCGAACA CCCGGCTGGT GCCTTAGCGA GACCTACCTG 
AAGTGCGGCA TGAACGCCCG TGAAGATAAT GTCTGGATCC CTGACGACAC CTACTACTGC 
AAACTCATCG GACGCCTCGT GGATACCATG GCAGGTAAAT CTCCAGGCCC GTTCCCCAAC 
TGTGACTGGA GGTTTAACGA GTTCCCGAAC CCGGCCGCTC ATGCGCTGCA CGTCACCTGT 
GTGGAGCTGA TGGCTCTGGC GGTCCCGGGG AAAGACGTGG GCAATGCTCT GCTTAATGTG 
GTGCTCAAGA GTCAGCCGCT GGTGCCTCGA GAGAACATCA CGGCCTGGAT GAACGCCATC 
GGACTGGTCA TCACCGCTCT GCCTGAGCCC TACTGGATCG TCCTGCATGA CCGTATCGTG 
AGTGTGATCA GCAGTGCGGT GCTCAGCTCT GGGTCAGATT GGCTGGGTTA CCCATTCCAG 
CTTCTGGACT TCACCGCTTG CCATCAGTCC TACTCAGAGA TGCACTGCAG TTACGTTCTG 
GCTCTGGCTC ACGCCGTGTG GCATCACTCC AGCATCGGCC AGCTCTCGCT CATACCCAAG 
TTCCTGTCGG AGGTCCTGAA GCCCATCGTG AAGACTGAGT TCCAGCTGCT GTACGTTTAT 
CATCTGGTGG GCCCGTTTCT GCAGCGCTTC CAGCAGGAGA GAACCAGATG CATGCTGGAG 
ATTGGAGTGG CGTTTTATGA GATGCTTCAG GCTGTGGATC AGCACAGCAA CCACCTGGCC 
TACATGGACC CCATCTGTGA CTTCCTGTAC CACATCAAAT ACATGTTTAC TGGGGACAGT 
GTCAAAGACC AGGTGGAGCG CATCATCTGT TCTCTGCGTC CAGCAATGCG TCTTCGTCTA 
CGCTTCATCA CACACATCAG TAAGATGGAG CCGGCGGCGG TGCCCGTGTC CAACAGCAGC 
AGCGTCCAGC AGACCAGCAG CGCCTCCTCA CCCACAGCAC AGAGCACTGC TGGAGCCGCC 
ATACCACTGT CTGTGACACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med23 ( XM_005160333.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160390.1 Danio rerio mediator complex subunit 23 (med23), transcript variant X1, mRNA.

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CloneID	ODa24589
Clone ID Related Accession (Same CDS sequence)	XM_021468405.1 , XM_005160333.3
Accession Version	XM_005160333.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mediator of RNA polymerase II transcription subunit 23 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160333.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACA CACACTTCTC ATCCATTCAT CACAGTAACA ACAACAGCAG CAGTCACATC 
TCAATCATTG AGAAAACGGA GGTGATCGAG GAGGCGTTTG CTGGCATGTT CATGGACAGC 
CCTGAGGATG AGAAGACCAA ACTCACAAGC TGTTTAGGAG CCTTTAGGCA GTTCTGGAGT 
ACCCTGCCGC AGGAGTCTCA TGAGCAGTGT GTGCAGTGGA TCGTGCGCTT CATTCATGGT 
CAGCACAGCC CCAAACGCAT CGCCTTCCTC TACGACTGTC TGGCCATGGC GGTGGAGACC 
AGCCTGCTGC CACCCAGGAT GGTGTGTCAG GCTCTCATCA GCTCCGACAG TCTGGAGTGG 
GAACGGACTC AACTCTGGGC TTTGACCTTC AAACTCATCC GCAAAATCAT CGGAGGAGTC 
GACTATAAGG GTGTGAGAGA TCTATTGAAG GCCGTGTTGG ACAAGATTCA GACCATCCCC 
AACTTTGTGA GCTCAGCTGT GGTTCAGCAG CTGCTCGCGG CACGAGAGGT TGTGGAGTAT 
ATACTGGACC GCAACGCCTG TCTGCTGCCG GCGTACTTCG CTGTGACTGA GATCCGGAAA 
CTTTGTCCAG AGGGGGCGCT CTCACACTGG CTGTTGGGCA GCCTGATATC TGACTTTGTC 
GACAGTTTCA GACCGACGGC ACGTATAAAC TCCATATGTG GGCGCTGCAG TCTTCTGCCT 
GTGGTCAATA ATTCTGGGGC CATCTGTAAC TCATGGAAGC TGGACCCCAC CACTCTGCGT 
TTCCCTCTGA GAGGAATGCT GCCGTATGAT AAGGACCTGT TTGATCCGCA GACAGGGTTG 
CTGAGGTATG TGCTGGAGCA GCCGTACTCC AGAGACATGG TGTGCAACAT GCTGGGCCTC 
AACAAACAGC ACAAGCAGAG GTGTCCGGTT CTGGAGGACC AGCTGGTGGA TCTGGTGGTT 
TATGCCATGG AGCGCTCTGA GACTGAGGAG CAGTTTGATG ACGGCGGCAC CAGCCAGCTC 
CTGTGGCAGC ATCTGTCCAG CCAGCTCATC TTCTTCGTCC TCTTCCAGTT TGCCAGCTTC 
CCTCACATGG TGCTGTCGCT CCATCAGAAG CTGGCAGGTC GGGGTTTGAT CAAGGGCAGG 
GATCATCTGA TGTGGGTTCT GCTGCAGTTC ATCTCCGGCA GCATTCAGAA GAACGCTCTG 
GGAGACTTTC TCCCCGTCAT GAAGCTCTTC GACCTGCTGT ACCCTGAGAA AGAGTGTATT 
CCAGTCCCGG ACATCAACAA GCCCCAGTCC ACACACGCAT TCGCCATGAC CTGCATCTGG 
ATTCACCTCA ACCGCAAAGC CCAGAACGAC AACTCCAAAC TGCAGATCCC CATCCCACAC 
TCACTGAAGC TGCACCATGA GTTTCTCCAG CAGAGTCTGC GCAATAAGAG TTTGCCCATG 
ACAGACTATA AAATCGCTCT GCTGTGTAAC GCCTACTCCA CCAACTCCGA GTGCTTCACG 
CTGCCCATGG GCGTCCTGGT GGAGACCATC TACGGCAATG GTAGCATGCG CATCACTCTG 
CCAGGCACCA ATTGCATGGC GTCCGGCTCT GTCACGCCAC TGCCCATGAA CCTGCTGGAC 
TCCCTAACCG TCCATGCTAA GATGAGTTTG ATCCACAGCA TCGCCACCCG TGTTATTAAA 
CTAGCCCACG CTAAGTCCAG TCTGGCTCTG GCTCCTGCGC TGGTGGAGAC GTACAGCCGG 
CTGCTGGTCT ACATGGAGAT TGAGTCGCTC GGCATCAAGG GATTCATCAG TCAGCTTCTG 
CCGAATGTGT TCAAGTCTCA CGCGTGGGGG ATTCTGCACA CACTGCTGGA GATGTTCAGC 
TACCGCATGC ACCACATTCA GCCTCATTAC CGCGTGCAGC TCCTGTCCCA TCTGCACTCG 
CTCGCCGCTG TCCCACAGAC CAATCAGAAC CAGCTGCACC TCTGTGTTGA AAGCACAGCT 
CTGCGCCTGA TCACGGCTCT GGGTAGTTCC GAGGTTCAGC CTCAGTTCAC ACGGTTCCTC 
AACGACCCAA AAACAGTGCT GTCGGCCGAG TCTGAGGAGC TAAACCGAGC ACTCATCCTC 
ACACTGGCCC GGGCCACTCA CGTCACAGAT TTCTTTACAG GCTCGGACTC GATTCAGGGC 
ACTTGGTGTA AAGACATCCT GCAGACCATC ATGAACTTCA CACCTCATAA CTGGGCCTCT 
CACACTCTTA GCTGTTTCCC TGCTCCACTG CAGGCGTTCT TCAAGCAGAA TAATGTCCCT 
CAGGAGAGCC GCTTCAACCT GAAGAAGAAC GTGGAGGAGG AGTACAGGAA GTGGAAGTCC 
ATGACCAATG AGAACGACAT CATCACCCAC TTCTCCATGC AGGGCTCGCC GCCGCTCTTC 
CTGTGTCTGC TGTGGAAGAT GCTGCTGGAG ACGGACCACA TCAACCAAAT CGGCTTCAGG 
GTTCTGGAGC GCATCGGGGC GCGAGCGCTG GTGGCTCATG TGCGCACGTT TGCCGACTTC 
CTGGTGTACG AGTTCTCCAC ATCCGCTGGC GGACAGCAGC TAAACAAGTG CATCGAGATC 
CTGAACGACA TGGTGTGGAA GTACAATATC GTGACCCTGG ATAGACTCAT CCTCTGCCTG 
GCGATGAGGA GTCACGAAGG GAACGAGGCT CAGGTCTGCT ACTTCATCAT CCAGCTTCTC 
CTGCTCAAAC CCAACGACTT CCGCAACCGC GTCAGTGACT TTGTGAAGGA GAACGCTCCA 
GAACACTGGC TGCAGAGCGA CTGGCACACC AAACACATGA CCTACCACAA GAAGTATCCA 
GAGAAGCTGT ATTTCGAGGG TCTGGCGGAG CAGGTCAATC CTCCTATCCA GCTGCAGTCT 
CAGTATCTGC CCATTTACTT CGGCAACGTG TGTCTGCGCT TCCTCCCCGT GTTCGACATC 
GTCATCCACC GCTTCCTGGA GCTGCTGCCG GTCTCCAAAT CCCTGGAGAC GCTGCTCGAT 
CATCTGGGAG GACTCTACAA GTTTCATGAT CGGCCGGTGA CGTACCTGTA CAACACGCTG 
CATTATTATG AGAGGCACCT ACGCGACCGC ACAAACCTGA AGAGGAAGCT GGTGCATGCC 
ATCATGAGCT CGCTGAAGGA CAATCGAACA CCCGGCTGGT GCCTTAGCGA GACCTACCTG 
AAGTGCGGCA TGAACGCCCG TGAAGATAAT GTCTGGATCC CTGACGACAC CTACTACTGC 
AAACTCATCG GACGCCTCGT GGATACCATG GCAGGTAAAT CTCCAGGCCC GTTCCCCAAC 
TGTGACTGGA GGTTTAACGA GTTCCCGAAC CCGGCCGCTC ATGCGCTGCA CGTCACCTGT 
GTGGAGCTGA TGGCTCTGGC GGTCCCGGGG AAAGACGTGG GCAATGCTCT GCTTAATGTG 
GTGCTCAAGA GTCAGCCGCT GGTGCCTCGA GAGAACATCA CGGCCTGGAT GAACGCCATC 
GGACTGGTCA TCACCGCTCT GCCTGAGCCC TACTGGATCG TCCTGCATGA CCGTATCGTG 
AGTGTGATCA GCAGTGCGGT GCTCAGCTCT GGGTCAGATT GGCTGGGTTA CCCATTCCAG 
CTTCTGGACT TCACCGCTTG CCATCAGTCC TACTCAGAGA TGCACTGCAG TTACGTTCTG 
GCTCTGGCTC ACGCCGTGTG GCATCACTCC AGCATCGGCC AGCTCTCGCT CATACCCAAG 
TTCCTGTCGG AGGTCCTGAA GCCCATCGTG AAGACTGAGT TCCAGCTGCT GTACGTTTAT 
CATCTGGTGG GCCCGTTTCT GCAGCGCTTC CAGCAGGAGA GAACCAGATG CATGCTGGAG 
ATTGGAGTGG CGTTTTATGA GATGCTTCAG GCTGTGGATC AGCACAGCAA CCACCTGGCC 
TACATGGACC CCATCTGTGA CTTCCTGTAC CACATCAAAT ACATGTTTAC TGGGGACAGT 
GTCAAAGACC AGGTGGAGCG CATCATCTGT TCTCTGCGTC CAGCAATGCG TCTTCGTCTA 
CGCTTCATCA CACACATCAG TAAGATGGAG CCGGCGGCGG TGCCCGTGTC CAACAGCAGC 
AGCGTCCAGC AGACCAGCAG CGCCTCCTCA CCCACAGCAC AGAGCACTGC TGGAGCCGCC 
ATACCACTGT CTGTGACACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160390.1
CDS	217..4380
Translation	MQHTHFSSIHHSNNNSSSHISIIEKTEVIEEAFAGMFMDSPEDEKTKLTSCLGAFRQFWSTLPQESHEQCVQWIVRFIHGQHSPKRIAFLYDCLAMAVETSLLPPRMVCQALISSDSLEWERTQLWALTFKLIRKIIGGVDYKGVRDLLKAVLDKIQTIPNFVSSAVVQQLLAAREVVEYILDRNACLLPAYFAVTEIRKLCPEGALSHWLLGSLISDFVDSFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPTTLRFPLRGMLPYDKDLFDPQTGLLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEQFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALGDFLPVMKLFDLLYPEKECIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLRNKSLPMTDYKIALLCNAYSTNSECFTLPMGVLVETIYGNGSMRITLPGTNCMASGSVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSLALAPALVETYSRLLVYMEIESLGIKGFISQLLPNVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHSLAAVPQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMNFTPHNWASHTLSCFPAPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMTNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGFRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENAPEHWLQSDWHTKHMTYHKKYPEKLYFEGLAEQVNPPIQLQSQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYERHLRDRTNLKRKLVHAIMSSLKDNRTPGWCLSETYLKCGMNAREDNVWIPDDTYYCKLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLVITALPEPYWIVLHDRIVSVISSAVLSSGSDWLGYPFQLLDFTACHQSYSEMHCSYVLALAHAVWHHSSIGQLSLIPKFLSEVLKPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMLEIGVAFYEMLQAVDQHSNHLAYMDPICDFLYHIKYMFTGDSVKDQVERIICSLRPAMRLRLRFITHISKMEPAAVPVSNSSSVQQTSSASSPTAQSTAGAAIPLSVTQ

Target ORF information:

RefSeq Version	XM_005160333.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mediator complex subunit 23 (med23), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160333.3

ORF Insert Sequence:

ATGCAGCACA CACACTTCTC ATCCATTCAT CACAGTAACA ACAACAGCAG CAGTCACATC 
TCAATCATTG AGAAAACGGA GGTGATCGAG GAGGCGTTTG CTGGCATGTT CATGGACAGC 
CCTGAGGATG AGAAGACCAA ACTCACAAGC TGTTTAGGAG CCTTTAGGCA GTTCTGGAGT 
ACCCTGCCGC AGGAGTCTCA TGAGCAGTGT GTGCAGTGGA TCGTGCGCTT CATTCATGGT 
CAGCACAGCC CCAAACGCAT CGCCTTCCTC TACGACTGTC TGGCCATGGC GGTGGAGACC 
AGCCTGCTGC CACCCAGGAT GGTGTGTCAG GCTCTCATCA GCTCCGACAG TCTGGAGTGG 
GAACGGACTC AACTCTGGGC TTTGACCTTC AAACTCATCC GCAAAATCAT CGGAGGAGTC 
GACTATAAGG GTGTGAGAGA TCTATTGAAG GCCGTGTTGG ACAAGATTCA GACCATCCCC 
AACTTTGTGA GCTCAGCTGT GGTTCAGCAG CTGCTCGCGG CACGAGAGGT TGTGGAGTAT 
ATACTGGACC GCAACGCCTG TCTGCTGCCG GCGTACTTCG CTGTGACTGA GATCCGGAAA 
CTTTGTCCAG AGGGGGCGCT CTCACACTGG CTGTTGGGCA GCCTGATATC TGACTTTGTC 
GACAGTTTCA GACCGACGGC ACGTATAAAC TCCATATGTG GGCGCTGCAG TCTTCTGCCT 
GTGGTCAATA ATTCTGGGGC CATCTGTAAC TCATGGAAGC TGGACCCCAC CACTCTGCGT 
TTCCCTCTGA GAGGAATGCT GCCGTATGAT AAGGACCTGT TTGATCCGCA GACAGGGTTG 
CTGAGGTATG TGCTGGAGCA GCCGTACTCC AGAGACATGG TGTGCAACAT GCTGGGCCTC 
AACAAACAGC ACAAGCAGAG GTGTCCGGTT CTGGAGGACC AGCTGGTGGA TCTGGTGGTT 
TATGCCATGG AGCGCTCTGA GACTGAGGAG CAGTTTGATG ACGGCGGCAC CAGCCAGCTC 
CTGTGGCAGC ATCTGTCCAG CCAGCTCATC TTCTTCGTCC TCTTCCAGTT TGCCAGCTTC 
CCTCACATGG TGCTGTCGCT CCATCAGAAG CTGGCAGGTC GGGGTTTGAT CAAGGGCAGG 
GATCATCTGA TGTGGGTTCT GCTGCAGTTC ATCTCCGGCA GCATTCAGAA GAACGCTCTG 
GGAGACTTTC TCCCCGTCAT GAAGCTCTTC GACCTGCTGT ACCCTGAGAA AGAGTGTATT 
CCAGTCCCGG ACATCAACAA GCCCCAGTCC ACACACGCAT TCGCCATGAC CTGCATCTGG 
ATTCACCTCA ACCGCAAAGC CCAGAACGAC AACTCCAAAC TGCAGATCCC CATCCCACAC 
TCACTGAAGC TGCACCATGA GTTTCTCCAG CAGAGTCTGC GCAATAAGAG TTTGCCCATG 
ACAGACTATA AAATCGCTCT GCTGTGTAAC GCCTACTCCA CCAACTCCGA GTGCTTCACG 
CTGCCCATGG GCGTCCTGGT GGAGACCATC TACGGCAATG GTAGCATGCG CATCACTCTG 
CCAGGCACCA ATTGCATGGC GTCCGGCTCT GTCACGCCAC TGCCCATGAA CCTGCTGGAC 
TCCCTAACCG TCCATGCTAA GATGAGTTTG ATCCACAGCA TCGCCACCCG TGTTATTAAA 
CTAGCCCACG CTAAGTCCAG TCTGGCTCTG GCTCCTGCGC TGGTGGAGAC GTACAGCCGG 
CTGCTGGTCT ACATGGAGAT TGAGTCGCTC GGCATCAAGG GATTCATCAG TCAGCTTCTG 
CCGAATGTGT TCAAGTCTCA CGCGTGGGGG ATTCTGCACA CACTGCTGGA GATGTTCAGC 
TACCGCATGC ACCACATTCA GCCTCATTAC CGCGTGCAGC TCCTGTCCCA TCTGCACTCG 
CTCGCCGCTG TCCCACAGAC CAATCAGAAC CAGCTGCACC TCTGTGTTGA AAGCACAGCT 
CTGCGCCTGA TCACGGCTCT GGGTAGTTCC GAGGTTCAGC CTCAGTTCAC ACGGTTCCTC 
AACGACCCAA AAACAGTGCT GTCGGCCGAG TCTGAGGAGC TAAACCGAGC ACTCATCCTC 
ACACTGGCCC GGGCCACTCA CGTCACAGAT TTCTTTACAG GCTCGGACTC GATTCAGGGC 
ACTTGGTGTA AAGACATCCT GCAGACCATC ATGAACTTCA CACCTCATAA CTGGGCCTCT 
CACACTCTTA GCTGTTTCCC TGCTCCACTG CAGGCGTTCT TCAAGCAGAA TAATGTCCCT 
CAGGAGAGCC GCTTCAACCT GAAGAAGAAC GTGGAGGAGG AGTACAGGAA GTGGAAGTCC 
ATGACCAATG AGAACGACAT CATCACCCAC TTCTCCATGC AGGGCTCGCC GCCGCTCTTC 
CTGTGTCTGC TGTGGAAGAT GCTGCTGGAG ACGGACCACA TCAACCAAAT CGGCTTCAGG 
GTTCTGGAGC GCATCGGGGC GCGAGCGCTG GTGGCTCATG TGCGCACGTT TGCCGACTTC 
CTGGTGTACG AGTTCTCCAC ATCCGCTGGC GGACAGCAGC TAAACAAGTG CATCGAGATC 
CTGAACGACA TGGTGTGGAA GTACAATATC GTGACCCTGG ATAGACTCAT CCTCTGCCTG 
GCGATGAGGA GTCACGAAGG GAACGAGGCT CAGGTCTGCT ACTTCATCAT CCAGCTTCTC 
CTGCTCAAAC CCAACGACTT CCGCAACCGC GTCAGTGACT TTGTGAAGGA GAACGCTCCA 
GAACACTGGC TGCAGAGCGA CTGGCACACC AAACACATGA CCTACCACAA GAAGTATCCA 
GAGAAGCTGT ATTTCGAGGG TCTGGCGGAG CAGGTCAATC CTCCTATCCA GCTGCAGTCT 
CAGTATCTGC CCATTTACTT CGGCAACGTG TGTCTGCGCT TCCTCCCCGT GTTCGACATC 
GTCATCCACC GCTTCCTGGA GCTGCTGCCG GTCTCCAAAT CCCTGGAGAC GCTGCTCGAT 
CATCTGGGAG GACTCTACAA GTTTCATGAT CGGCCGGTGA CGTACCTGTA CAACACGCTG 
CATTATTATG AGAGGCACCT ACGCGACCGC ACAAACCTGA AGAGGAAGCT GGTGCATGCC 
ATCATGAGCT CGCTGAAGGA CAATCGAACA CCCGGCTGGT GCCTTAGCGA GACCTACCTG 
AAGTGCGGCA TGAACGCCCG TGAAGATAAT GTCTGGATCC CTGACGACAC CTACTACTGC 
AAACTCATCG GACGCCTCGT GGATACCATG GCAGGTAAAT CTCCAGGCCC GTTCCCCAAC 
TGTGACTGGA GGTTTAACGA GTTCCCGAAC CCGGCCGCTC ATGCGCTGCA CGTCACCTGT 
GTGGAGCTGA TGGCTCTGGC GGTCCCGGGG AAAGACGTGG GCAATGCTCT GCTTAATGTG 
GTGCTCAAGA GTCAGCCGCT GGTGCCTCGA GAGAACATCA CGGCCTGGAT GAACGCCATC 
GGACTGGTCA TCACCGCTCT GCCTGAGCCC TACTGGATCG TCCTGCATGA CCGTATCGTG 
AGTGTGATCA GCAGTGCGGT GCTCAGCTCT GGGTCAGATT GGCTGGGTTA CCCATTCCAG 
CTTCTGGACT TCACCGCTTG CCATCAGTCC TACTCAGAGA TGCACTGCAG TTACGTTCTG 
GCTCTGGCTC ACGCCGTGTG GCATCACTCC AGCATCGGCC AGCTCTCGCT CATACCCAAG 
TTCCTGTCGG AGGTCCTGAA GCCCATCGTG AAGACTGAGT TCCAGCTGCT GTACGTTTAT 
CATCTGGTGG GCCCGTTTCT GCAGCGCTTC CAGCAGGAGA GAACCAGATG CATGCTGGAG 
ATTGGAGTGG CGTTTTATGA GATGCTTCAG GCTGTGGATC AGCACAGCAA CCACCTGGCC 
TACATGGACC CCATCTGTGA CTTCCTGTAC CACATCAAAT ACATGTTTAC TGGGGACAGT 
GTCAAAGACC AGGTGGAGCG CATCATCTGT TCTCTGCGTC CAGCAATGCG TCTTCGTCTA 
CGCTTCATCA CACACATCAG TAAGATGGAG CCGGCGGCGG TGCCCGTGTC CAACAGCAGC 
AGCGTCCAGC AGACCAGCAG CGCCTCCTCA CCCACAGCAC AGAGCACTGC TGGAGCCGCC 
ATACCACTGT CTGTGACACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med23 ( XM_005160334.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160391.1 Danio rerio mediator complex subunit 23 (med23), transcript variant X3, mRNA.

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CloneID	ODa24590
Clone ID Related Accession (Same CDS sequence)	XM_005160334.3 , XM_005160334.4
Accession Version	XM_005160334.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mediator of RNA polymerase II transcription subunit 23 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160334.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTCC CGATGGAGAC TCAGCTGCAG AGCATCTTTG AGGATGTAGT GAAAACGGAG 
GTGATCGAGG AGGCGTTTGC TGGCATGTTC ATGGACAGCC CTGAGGATGA GAAGACCAAA 
CTCACAAGCT GTTTAGGAGC CTTTAGGCAG TTCTGGAGTA CCCTGCCGCA GGAGTCTCAT 
GAGCAGTGTG TGCAGTGGAT CGTGCGCTTC ATTCATGGTC AGCACAGCCC CAAACGCATC 
GCCTTCCTCT ACGACTGTCT GGCCATGGCG GTGGAGACCA GCCTGCTGCC ACCCAGGATG 
GTGTGTCAGG CTCTCATCAG CTCCGACAGT CTGGAGTGGG AACGGACTCA ACTCTGGGCT 
TTGACCTTCA AACTCATCCG CAAAATCATC GGAGGAGTCG ACTATAAGGG TGTGAGAGAT 
CTATTGAAGG CCGTGTTGGA CAAGATTCAG ACCATCCCCA ACTTTGTGAG CTCAGCTGTG 
GTTCAGCAGC TGCTCGCGGC ACGAGAGGTT GTGGAGTATA TACTGGACCG CAACGCCTGT 
CTGCTGCCGG CGTACTTCGC TGTGACTGAG ATCCGGAAAC TTTGTCCAGA GGGGGCGCTC 
TCACACTGGC TGTTGGGCAG CCTGATATCT GACTTTGTCG ACAGTTTCAG ACCGACGGCA 
CGTATAAACT CCATATGTGG GCGCTGCAGT CTTCTGCCTG TGGTCAATAA TTCTGGGGCC 
ATCTGTAACT CATGGAAGCT GGACCCCACC ACTCTGCGTT TCCCTCTGAG AGGAATGCTG 
CCGTATGATA AGGACCTGTT TGATCCGCAG ACAGGGTTGC TGAGGTATGT GCTGGAGCAG 
CCGTACTCCA GAGACATGGT GTGCAACATG CTGGGCCTCA ACAAACAGCA CAAGCAGAGG 
TGTCCGGTTC TGGAGGACCA GCTGGTGGAT CTGGTGGTTT ATGCCATGGA GCGCTCTGAG 
ACTGAGGAGC AGTTTGATGA CGGCGGCACC AGCCAGCTCC TGTGGCAGCA TCTGTCCAGC 
CAGCTCATCT TCTTCGTCCT CTTCCAGTTT GCCAGCTTCC CTCACATGGT GCTGTCGCTC 
CATCAGAAGC TGGCAGGTCG GGGTTTGATC AAGGGCAGGG ATCATCTGAT GTGGGTTCTG 
CTGCAGTTCA TCTCCGGCAG CATTCAGAAG AACGCTCTGG GAGACTTTCT CCCCGTCATG 
AAGCTCTTCG ACCTGCTGTA CCCTGAGAAA GAGTGTATTC CAGTCCCGGA CATCAACAAG 
CCCCAGTCCA CACACGCATT CGCCATGACC TGCATCTGGA TTCACCTCAA CCGCAAAGCC 
CAGAACGACA ACTCCAAACT GCAGATCCCC ATCCCACACT CACTGAAGCT GCACCATGAG 
TTTCTCCAGC AGAGTCTGCG CAATAAGAGT TTGCCCATGA CAGACTATAA AATCGCTCTG 
CTGTGTAACG CCTACTCCAC CAACTCCGAG TGCTTCACGC TGCCCATGGG CGTCCTGGTG 
GAGACCATCT ACGGCAATGG TAGCATGCGC ATCACTCTGC CAGGCACCAA TTGCATGGCG 
TCCGGCTCTG TCACGCCACT GCCCATGAAC CTGCTGGACT CCCTAACCGT CCATGCTAAG 
ATGAGTTTGA TCCACAGCAT CGCCACCCGT GTTATTAAAC TAGCCCACGC TAAGTCCAGT 
CTGGCTCTGG CTCCTGCGCT GGTGGAGACG TACAGCCGGC TGCTGGTCTA CATGGAGATT 
GAGTCGCTCG GCATCAAGGG ATTCATCAGT CAGCTTCTGC CGAATGTGTT CAAGTCTCAC 
GCGTGGGGGA TTCTGCACAC ACTGCTGGAG ATGTTCAGCT ACCGCATGCA CCACATTCAG 
CCTCATTACC GCGTGCAGCT CCTGTCCCAT CTGCACTCGC TCGCCGCTGT CCCACAGACC 
AATCAGAACC AGCTGCACCT CTGTGTTGAA AGCACAGCTC TGCGCCTGAT CACGGCTCTG 
GGTAGTTCCG AGGTTCAGCC TCAGTTCACA CGGTTCCTCA ACGACCCAAA AACAGTGCTG 
TCGGCCGAGT CTGAGGAGCT AAACCGAGCA CTCATCCTCA CACTGGCCCG GGCCACTCAC 
GTCACAGATT TCTTTACAGG CTCGGACTCG ATTCAGGGCA CTTGGTGTAA AGACATCCTG 
CAGACCATCA TGAACTTCAC ACCTCATAAC TGGGCCTCTC ACACTCTTAG CTGTTTCCCT 
GCTCCACTGC AGGCGTTCTT CAAGCAGAAT AATGTCCCTC AGGAGAGCCG CTTCAACCTG 
AAGAAGAACG TGGAGGAGGA GTACAGGAAG TGGAAGTCCA TGACCAATGA GAACGACATC 
ATCACCCACT TCTCCATGCA GGGCTCGCCG CCGCTCTTCC TGTGTCTGCT GTGGAAGATG 
CTGCTGGAGA CGGACCACAT CAACCAAATC GGCTTCAGGG TTCTGGAGCG CATCGGGGCG 
CGAGCGCTGG TGGCTCATGT GCGCACGTTT GCCGACTTCC TGGTGTACGA GTTCTCCACA 
TCCGCTGGCG GACAGCAGCT AAACAAGTGC ATCGAGATCC TGAACGACAT GGTGTGGAAG 
TACAATATCG TGACCCTGGA TAGACTCATC CTCTGCCTGG CGATGAGGAG TCACGAAGGG 
AACGAGGCTC AGGTCTGCTA CTTCATCATC CAGCTTCTCC TGCTCAAACC CAACGACTTC 
CGCAACCGCG TCAGTGACTT TGTGAAGGAG AACGCTCCAG AACACTGGCT GCAGAGCGAC 
TGGCACACCA AACACATGAC CTACCACAAG AAGTATCCAG AGAAGCTGTA TTTCGAGGGT 
CTGGCGGAGC AGGTCAATCC TCCTATCCAG CTGCAGTCTC AGTATCTGCC CATTTACTTC 
GGCAACGTGT GTCTGCGCTT CCTCCCCGTG TTCGACATCG TCATCCACCG CTTCCTGGAG 
CTGCTGCCGG TCTCCAAATC CCTGGAGACG CTGCTCGATC ATCTGGGAGG ACTCTACAAG 
TTTCATGATC GGCCGGTGAC GTACCTGTAC AACACGCTGC ATTATTATGA GAGGCACCTA 
CGCGACCGCA CAAACCTGAA GAGGAAGCTG GTGCATGCCA TCATGAGCTC GCTGAAGGAC 
AATCGAACAC CCGGCTGGTG CCTTAGCGAG ACCTACCTGA AGTGCGGCAT GAACGCCCGT 
GAAGATAATG TCTGGATCCC TGACGACACC TACTACTGCA AACTCATCGG ACGCCTCGTG 
GATACCATGG CAGGTAAATC TCCAGGCCCG TTCCCCAACT GTGACTGGAG GTTTAACGAG 
TTCCCGAACC CGGCCGCTCA TGCGCTGCAC GTCACCTGTG TGGAGCTGAT GGCTCTGGCG 
GTCCCGGGGA AAGACGTGGG CAATGCTCTG CTTAATGTGG TGCTCAAGAG TCAGCCGCTG 
GTGCCTCGAG AGAACATCAC GGCCTGGATG AACGCCATCG GACTGGTCAT CACCGCTCTG 
CCTGAGCCCT ACTGGATCGT CCTGCATGAC CGTATCGTGA GTGTGATCAG CAGTGCGGTG 
CTCAGCTCTG GGTCAGATTG GCTGGGTTAC CCATTCCAGC TTCTGGACTT CACCGCTTGC 
CATCAGTCCT ACTCAGAGAT GCACTGCAGT TACGTTCTGG CTCTGGCTCA CGCCGTGTGG 
CATCACTCCA GCATCGGCCA GCTCTCGCTC ATACCCAAGT TCCTGTCGGA GGTCCTGAAG 
CCCATCGTGA AGACTGAGTT CCAGCTGCTG TACGTTTATC ATCTGGTGGG CCCGTTTCTG 
CAGCGCTTCC AGCAGGAGAG AACCAGATGC ATGCTGGAGA TTGGAGTGGC GTTTTATGAG 
ATGCTTCAGG CTGTGGATCA GCACAGCAAC CACCTGGCCT ACATGGACCC CATCTGTGAC 
TTCCTGTACC ACATCAAATA CATGTTTACT GGGGACAGTG TCAAAGACCA GGTGGAGCGC 
ATCATCTGTT CTCTGCGTCC AGCAATGCGT CTTCGTCTAC GCTTCATCAC ACACATCAGT 
AAGATGGAGC CGGCGGCGGT GCCCGTGTCC AACAGCAGCA GCGTCCAGCA GACCAGCAGC 
GCCTCCTCAC CCACAGCACA GAGCACTGCT GGAGCCGCCA TACCACTGTC TGTGACACAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160391.1
CDS	159..4301
Translation	MAVPMETQLQSIFEDVVKTEVIEEAFAGMFMDSPEDEKTKLTSCLGAFRQFWSTLPQESHEQCVQWIVRFIHGQHSPKRIAFLYDCLAMAVETSLLPPRMVCQALISSDSLEWERTQLWALTFKLIRKIIGGVDYKGVRDLLKAVLDKIQTIPNFVSSAVVQQLLAAREVVEYILDRNACLLPAYFAVTEIRKLCPEGALSHWLLGSLISDFVDSFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPTTLRFPLRGMLPYDKDLFDPQTGLLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEQFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALGDFLPVMKLFDLLYPEKECIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLRNKSLPMTDYKIALLCNAYSTNSECFTLPMGVLVETIYGNGSMRITLPGTNCMASGSVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSLALAPALVETYSRLLVYMEIESLGIKGFISQLLPNVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHSLAAVPQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMNFTPHNWASHTLSCFPAPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMTNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGFRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENAPEHWLQSDWHTKHMTYHKKYPEKLYFEGLAEQVNPPIQLQSQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYERHLRDRTNLKRKLVHAIMSSLKDNRTPGWCLSETYLKCGMNAREDNVWIPDDTYYCKLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLVITALPEPYWIVLHDRIVSVISSAVLSSGSDWLGYPFQLLDFTACHQSYSEMHCSYVLALAHAVWHHSSIGQLSLIPKFLSEVLKPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMLEIGVAFYEMLQAVDQHSNHLAYMDPICDFLYHIKYMFTGDSVKDQVERIICSLRPAMRLRLRFITHISKMEPAAVPVSNSSSVQQTSSASSPTAQSTAGAAIPLSVTQ

Target ORF information:

RefSeq Version	XM_005160334.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mediator complex subunit 23 (med23), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160334.3

ORF Insert Sequence:

ATGGCGGTCC CGATGGAGAC TCAGCTGCAG AGCATCTTTG AGGATGTAGT GAAAACGGAG 
GTGATCGAGG AGGCGTTTGC TGGCATGTTC ATGGACAGCC CTGAGGATGA GAAGACCAAA 
CTCACAAGCT GTTTAGGAGC CTTTAGGCAG TTCTGGAGTA CCCTGCCGCA GGAGTCTCAT 
GAGCAGTGTG TGCAGTGGAT CGTGCGCTTC ATTCATGGTC AGCACAGCCC CAAACGCATC 
GCCTTCCTCT ACGACTGTCT GGCCATGGCG GTGGAGACCA GCCTGCTGCC ACCCAGGATG 
GTGTGTCAGG CTCTCATCAG CTCCGACAGT CTGGAGTGGG AACGGACTCA ACTCTGGGCT 
TTGACCTTCA AACTCATCCG CAAAATCATC GGAGGAGTCG ACTATAAGGG TGTGAGAGAT 
CTATTGAAGG CCGTGTTGGA CAAGATTCAG ACCATCCCCA ACTTTGTGAG CTCAGCTGTG 
GTTCAGCAGC TGCTCGCGGC ACGAGAGGTT GTGGAGTATA TACTGGACCG CAACGCCTGT 
CTGCTGCCGG CGTACTTCGC TGTGACTGAG ATCCGGAAAC TTTGTCCAGA GGGGGCGCTC 
TCACACTGGC TGTTGGGCAG CCTGATATCT GACTTTGTCG ACAGTTTCAG ACCGACGGCA 
CGTATAAACT CCATATGTGG GCGCTGCAGT CTTCTGCCTG TGGTCAATAA TTCTGGGGCC 
ATCTGTAACT CATGGAAGCT GGACCCCACC ACTCTGCGTT TCCCTCTGAG AGGAATGCTG 
CCGTATGATA AGGACCTGTT TGATCCGCAG ACAGGGTTGC TGAGGTATGT GCTGGAGCAG 
CCGTACTCCA GAGACATGGT GTGCAACATG CTGGGCCTCA ACAAACAGCA CAAGCAGAGG 
TGTCCGGTTC TGGAGGACCA GCTGGTGGAT CTGGTGGTTT ATGCCATGGA GCGCTCTGAG 
ACTGAGGAGC AGTTTGATGA CGGCGGCACC AGCCAGCTCC TGTGGCAGCA TCTGTCCAGC 
CAGCTCATCT TCTTCGTCCT CTTCCAGTTT GCCAGCTTCC CTCACATGGT GCTGTCGCTC 
CATCAGAAGC TGGCAGGTCG GGGTTTGATC AAGGGCAGGG ATCATCTGAT GTGGGTTCTG 
CTGCAGTTCA TCTCCGGCAG CATTCAGAAG AACGCTCTGG GAGACTTTCT CCCCGTCATG 
AAGCTCTTCG ACCTGCTGTA CCCTGAGAAA GAGTGTATTC CAGTCCCGGA CATCAACAAG 
CCCCAGTCCA CACACGCATT CGCCATGACC TGCATCTGGA TTCACCTCAA CCGCAAAGCC 
CAGAACGACA ACTCCAAACT GCAGATCCCC ATCCCACACT CACTGAAGCT GCACCATGAG 
TTTCTCCAGC AGAGTCTGCG CAATAAGAGT TTGCCCATGA CAGACTATAA AATCGCTCTG 
CTGTGTAACG CCTACTCCAC CAACTCCGAG TGCTTCACGC TGCCCATGGG CGTCCTGGTG 
GAGACCATCT ACGGCAATGG TAGCATGCGC ATCACTCTGC CAGGCACCAA TTGCATGGCG 
TCCGGCTCTG TCACGCCACT GCCCATGAAC CTGCTGGACT CCCTAACCGT CCATGCTAAG 
ATGAGTTTGA TCCACAGCAT CGCCACCCGT GTTATTAAAC TAGCCCACGC TAAGTCCAGT 
CTGGCTCTGG CTCCTGCGCT GGTGGAGACG TACAGCCGGC TGCTGGTCTA CATGGAGATT 
GAGTCGCTCG GCATCAAGGG ATTCATCAGT CAGCTTCTGC CGAATGTGTT CAAGTCTCAC 
GCGTGGGGGA TTCTGCACAC ACTGCTGGAG ATGTTCAGCT ACCGCATGCA CCACATTCAG 
CCTCATTACC GCGTGCAGCT CCTGTCCCAT CTGCACTCGC TCGCCGCTGT CCCACAGACC 
AATCAGAACC AGCTGCACCT CTGTGTTGAA AGCACAGCTC TGCGCCTGAT CACGGCTCTG 
GGTAGTTCCG AGGTTCAGCC TCAGTTCACA CGGTTCCTCA ACGACCCAAA AACAGTGCTG 
TCGGCCGAGT CTGAGGAGCT AAACCGAGCA CTCATCCTCA CACTGGCCCG GGCCACTCAC 
GTCACAGATT TCTTTACAGG CTCGGACTCG ATTCAGGGCA CTTGGTGTAA AGACATCCTG 
CAGACCATCA TGAACTTCAC ACCTCATAAC TGGGCCTCTC ACACTCTTAG CTGTTTCCCT 
GCTCCACTGC AGGCGTTCTT CAAGCAGAAT AATGTCCCTC AGGAGAGCCG CTTCAACCTG 
AAGAAGAACG TGGAGGAGGA GTACAGGAAG TGGAAGTCCA TGACCAATGA GAACGACATC 
ATCACCCACT TCTCCATGCA GGGCTCGCCG CCGCTCTTCC TGTGTCTGCT GTGGAAGATG 
CTGCTGGAGA CGGACCACAT CAACCAAATC GGCTTCAGGG TTCTGGAGCG CATCGGGGCG 
CGAGCGCTGG TGGCTCATGT GCGCACGTTT GCCGACTTCC TGGTGTACGA GTTCTCCACA 
TCCGCTGGCG GACAGCAGCT AAACAAGTGC ATCGAGATCC TGAACGACAT GGTGTGGAAG 
TACAATATCG TGACCCTGGA TAGACTCATC CTCTGCCTGG CGATGAGGAG TCACGAAGGG 
AACGAGGCTC AGGTCTGCTA CTTCATCATC CAGCTTCTCC TGCTCAAACC CAACGACTTC 
CGCAACCGCG TCAGTGACTT TGTGAAGGAG AACGCTCCAG AACACTGGCT GCAGAGCGAC 
TGGCACACCA AACACATGAC CTACCACAAG AAGTATCCAG AGAAGCTGTA TTTCGAGGGT 
CTGGCGGAGC AGGTCAATCC TCCTATCCAG CTGCAGTCTC AGTATCTGCC CATTTACTTC 
GGCAACGTGT GTCTGCGCTT CCTCCCCGTG TTCGACATCG TCATCCACCG CTTCCTGGAG 
CTGCTGCCGG TCTCCAAATC CCTGGAGACG CTGCTCGATC ATCTGGGAGG ACTCTACAAG 
TTTCATGATC GGCCGGTGAC GTACCTGTAC AACACGCTGC ATTATTATGA GAGGCACCTA 
CGCGACCGCA CAAACCTGAA GAGGAAGCTG GTGCATGCCA TCATGAGCTC GCTGAAGGAC 
AATCGAACAC CCGGCTGGTG CCTTAGCGAG ACCTACCTGA AGTGCGGCAT GAACGCCCGT 
GAAGATAATG TCTGGATCCC TGACGACACC TACTACTGCA AACTCATCGG ACGCCTCGTG 
GATACCATGG CAGGTAAATC TCCAGGCCCG TTCCCCAACT GTGACTGGAG GTTTAACGAG 
TTCCCGAACC CGGCCGCTCA TGCGCTGCAC GTCACCTGTG TGGAGCTGAT GGCTCTGGCG 
GTCCCGGGGA AAGACGTGGG CAATGCTCTG CTTAATGTGG TGCTCAAGAG TCAGCCGCTG 
GTGCCTCGAG AGAACATCAC GGCCTGGATG AACGCCATCG GACTGGTCAT CACCGCTCTG 
CCTGAGCCCT ACTGGATCGT CCTGCATGAC CGTATCGTGA GTGTGATCAG CAGTGCGGTG 
CTCAGCTCTG GGTCAGATTG GCTGGGTTAC CCATTCCAGC TTCTGGACTT CACCGCTTGC 
CATCAGTCCT ACTCAGAGAT GCACTGCAGT TACGTTCTGG CTCTGGCTCA CGCCGTGTGG 
CATCACTCCA GCATCGGCCA GCTCTCGCTC ATACCCAAGT TCCTGTCGGA GGTCCTGAAG 
CCCATCGTGA AGACTGAGTT CCAGCTGCTG TACGTTTATC ATCTGGTGGG CCCGTTTCTG 
CAGCGCTTCC AGCAGGAGAG AACCAGATGC ATGCTGGAGA TTGGAGTGGC GTTTTATGAG 
ATGCTTCAGG CTGTGGATCA GCACAGCAAC CACCTGGCCT ACATGGACCC CATCTGTGAC 
TTCCTGTACC ACATCAAATA CATGTTTACT GGGGACAGTG TCAAAGACCA GGTGGAGCGC 
ATCATCTGTT CTCTGCGTCC AGCAATGCGT CTTCGTCTAC GCTTCATCAC ACACATCAGT 
AAGATGGAGC CGGCGGCGGT GCCCGTGTCC AACAGCAGCA GCGTCCAGCA GACCAGCAGC 
GCCTCCTCAC CCACAGCACA GAGCACTGCT GGAGCCGCCA TACCACTGTC TGTGACACAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med23 ( XM_005160334.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160391.1 Danio rerio mediator complex subunit 23 (med23), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa24590
Clone ID Related Accession (Same CDS sequence)	XM_005160334.3 , XM_005160334.4
Accession Version	XM_005160334.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mediator of RNA polymerase II transcription subunit 23 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160334.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTCC CGATGGAGAC TCAGCTGCAG AGCATCTTTG AGGATGTAGT GAAAACGGAG 
GTGATCGAGG AGGCGTTTGC TGGCATGTTC ATGGACAGCC CTGAGGATGA GAAGACCAAA 
CTCACAAGCT GTTTAGGAGC CTTTAGGCAG TTCTGGAGTA CCCTGCCGCA GGAGTCTCAT 
GAGCAGTGTG TGCAGTGGAT CGTGCGCTTC ATTCATGGTC AGCACAGCCC CAAACGCATC 
GCCTTCCTCT ACGACTGTCT GGCCATGGCG GTGGAGACCA GCCTGCTGCC ACCCAGGATG 
GTGTGTCAGG CTCTCATCAG CTCCGACAGT CTGGAGTGGG AACGGACTCA ACTCTGGGCT 
TTGACCTTCA AACTCATCCG CAAAATCATC GGAGGAGTCG ACTATAAGGG TGTGAGAGAT 
CTATTGAAGG CCGTGTTGGA CAAGATTCAG ACCATCCCCA ACTTTGTGAG CTCAGCTGTG 
GTTCAGCAGC TGCTCGCGGC ACGAGAGGTT GTGGAGTATA TACTGGACCG CAACGCCTGT 
CTGCTGCCGG CGTACTTCGC TGTGACTGAG ATCCGGAAAC TTTGTCCAGA GGGGGCGCTC 
TCACACTGGC TGTTGGGCAG CCTGATATCT GACTTTGTCG ACAGTTTCAG ACCGACGGCA 
CGTATAAACT CCATATGTGG GCGCTGCAGT CTTCTGCCTG TGGTCAATAA TTCTGGGGCC 
ATCTGTAACT CATGGAAGCT GGACCCCACC ACTCTGCGTT TCCCTCTGAG AGGAATGCTG 
CCGTATGATA AGGACCTGTT TGATCCGCAG ACAGGGTTGC TGAGGTATGT GCTGGAGCAG 
CCGTACTCCA GAGACATGGT GTGCAACATG CTGGGCCTCA ACAAACAGCA CAAGCAGAGG 
TGTCCGGTTC TGGAGGACCA GCTGGTGGAT CTGGTGGTTT ATGCCATGGA GCGCTCTGAG 
ACTGAGGAGC AGTTTGATGA CGGCGGCACC AGCCAGCTCC TGTGGCAGCA TCTGTCCAGC 
CAGCTCATCT TCTTCGTCCT CTTCCAGTTT GCCAGCTTCC CTCACATGGT GCTGTCGCTC 
CATCAGAAGC TGGCAGGTCG GGGTTTGATC AAGGGCAGGG ATCATCTGAT GTGGGTTCTG 
CTGCAGTTCA TCTCCGGCAG CATTCAGAAG AACGCTCTGG GAGACTTTCT CCCCGTCATG 
AAGCTCTTCG ACCTGCTGTA CCCTGAGAAA GAGTGTATTC CAGTCCCGGA CATCAACAAG 
CCCCAGTCCA CACACGCATT CGCCATGACC TGCATCTGGA TTCACCTCAA CCGCAAAGCC 
CAGAACGACA ACTCCAAACT GCAGATCCCC ATCCCACACT CACTGAAGCT GCACCATGAG 
TTTCTCCAGC AGAGTCTGCG CAATAAGAGT TTGCCCATGA CAGACTATAA AATCGCTCTG 
CTGTGTAACG CCTACTCCAC CAACTCCGAG TGCTTCACGC TGCCCATGGG CGTCCTGGTG 
GAGACCATCT ACGGCAATGG TAGCATGCGC ATCACTCTGC CAGGCACCAA TTGCATGGCG 
TCCGGCTCTG TCACGCCACT GCCCATGAAC CTGCTGGACT CCCTAACCGT CCATGCTAAG 
ATGAGTTTGA TCCACAGCAT CGCCACCCGT GTTATTAAAC TAGCCCACGC TAAGTCCAGT 
CTGGCTCTGG CTCCTGCGCT GGTGGAGACG TACAGCCGGC TGCTGGTCTA CATGGAGATT 
GAGTCGCTCG GCATCAAGGG ATTCATCAGT CAGCTTCTGC CGAATGTGTT CAAGTCTCAC 
GCGTGGGGGA TTCTGCACAC ACTGCTGGAG ATGTTCAGCT ACCGCATGCA CCACATTCAG 
CCTCATTACC GCGTGCAGCT CCTGTCCCAT CTGCACTCGC TCGCCGCTGT CCCACAGACC 
AATCAGAACC AGCTGCACCT CTGTGTTGAA AGCACAGCTC TGCGCCTGAT CACGGCTCTG 
GGTAGTTCCG AGGTTCAGCC TCAGTTCACA CGGTTCCTCA ACGACCCAAA AACAGTGCTG 
TCGGCCGAGT CTGAGGAGCT AAACCGAGCA CTCATCCTCA CACTGGCCCG GGCCACTCAC 
GTCACAGATT TCTTTACAGG CTCGGACTCG ATTCAGGGCA CTTGGTGTAA AGACATCCTG 
CAGACCATCA TGAACTTCAC ACCTCATAAC TGGGCCTCTC ACACTCTTAG CTGTTTCCCT 
GCTCCACTGC AGGCGTTCTT CAAGCAGAAT AATGTCCCTC AGGAGAGCCG CTTCAACCTG 
AAGAAGAACG TGGAGGAGGA GTACAGGAAG TGGAAGTCCA TGACCAATGA GAACGACATC 
ATCACCCACT TCTCCATGCA GGGCTCGCCG CCGCTCTTCC TGTGTCTGCT GTGGAAGATG 
CTGCTGGAGA CGGACCACAT CAACCAAATC GGCTTCAGGG TTCTGGAGCG CATCGGGGCG 
CGAGCGCTGG TGGCTCATGT GCGCACGTTT GCCGACTTCC TGGTGTACGA GTTCTCCACA 
TCCGCTGGCG GACAGCAGCT AAACAAGTGC ATCGAGATCC TGAACGACAT GGTGTGGAAG 
TACAATATCG TGACCCTGGA TAGACTCATC CTCTGCCTGG CGATGAGGAG TCACGAAGGG 
AACGAGGCTC AGGTCTGCTA CTTCATCATC CAGCTTCTCC TGCTCAAACC CAACGACTTC 
CGCAACCGCG TCAGTGACTT TGTGAAGGAG AACGCTCCAG AACACTGGCT GCAGAGCGAC 
TGGCACACCA AACACATGAC CTACCACAAG AAGTATCCAG AGAAGCTGTA TTTCGAGGGT 
CTGGCGGAGC AGGTCAATCC TCCTATCCAG CTGCAGTCTC AGTATCTGCC CATTTACTTC 
GGCAACGTGT GTCTGCGCTT CCTCCCCGTG TTCGACATCG TCATCCACCG CTTCCTGGAG 
CTGCTGCCGG TCTCCAAATC CCTGGAGACG CTGCTCGATC ATCTGGGAGG ACTCTACAAG 
TTTCATGATC GGCCGGTGAC GTACCTGTAC AACACGCTGC ATTATTATGA GAGGCACCTA 
CGCGACCGCA CAAACCTGAA GAGGAAGCTG GTGCATGCCA TCATGAGCTC GCTGAAGGAC 
AATCGAACAC CCGGCTGGTG CCTTAGCGAG ACCTACCTGA AGTGCGGCAT GAACGCCCGT 
GAAGATAATG TCTGGATCCC TGACGACACC TACTACTGCA AACTCATCGG ACGCCTCGTG 
GATACCATGG CAGGTAAATC TCCAGGCCCG TTCCCCAACT GTGACTGGAG GTTTAACGAG 
TTCCCGAACC CGGCCGCTCA TGCGCTGCAC GTCACCTGTG TGGAGCTGAT GGCTCTGGCG 
GTCCCGGGGA AAGACGTGGG CAATGCTCTG CTTAATGTGG TGCTCAAGAG TCAGCCGCTG 
GTGCCTCGAG AGAACATCAC GGCCTGGATG AACGCCATCG GACTGGTCAT CACCGCTCTG 
CCTGAGCCCT ACTGGATCGT CCTGCATGAC CGTATCGTGA GTGTGATCAG CAGTGCGGTG 
CTCAGCTCTG GGTCAGATTG GCTGGGTTAC CCATTCCAGC TTCTGGACTT CACCGCTTGC 
CATCAGTCCT ACTCAGAGAT GCACTGCAGT TACGTTCTGG CTCTGGCTCA CGCCGTGTGG 
CATCACTCCA GCATCGGCCA GCTCTCGCTC ATACCCAAGT TCCTGTCGGA GGTCCTGAAG 
CCCATCGTGA AGACTGAGTT CCAGCTGCTG TACGTTTATC ATCTGGTGGG CCCGTTTCTG 
CAGCGCTTCC AGCAGGAGAG AACCAGATGC ATGCTGGAGA TTGGAGTGGC GTTTTATGAG 
ATGCTTCAGG CTGTGGATCA GCACAGCAAC CACCTGGCCT ACATGGACCC CATCTGTGAC 
TTCCTGTACC ACATCAAATA CATGTTTACT GGGGACAGTG TCAAAGACCA GGTGGAGCGC 
ATCATCTGTT CTCTGCGTCC AGCAATGCGT CTTCGTCTAC GCTTCATCAC ACACATCAGT 
AAGATGGAGC CGGCGGCGGT GCCCGTGTCC AACAGCAGCA GCGTCCAGCA GACCAGCAGC 
GCCTCCTCAC CCACAGCACA GAGCACTGCT GGAGCCGCCA TACCACTGTC TGTGACACAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160391.1
CDS	160..4302
Translation	MAVPMETQLQSIFEDVVKTEVIEEAFAGMFMDSPEDEKTKLTSCLGAFRQFWSTLPQESHEQCVQWIVRFIHGQHSPKRIAFLYDCLAMAVETSLLPPRMVCQALISSDSLEWERTQLWALTFKLIRKIIGGVDYKGVRDLLKAVLDKIQTIPNFVSSAVVQQLLAAREVVEYILDRNACLLPAYFAVTEIRKLCPEGALSHWLLGSLISDFVDSFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPTTLRFPLRGMLPYDKDLFDPQTGLLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEQFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALGDFLPVMKLFDLLYPEKECIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLRNKSLPMTDYKIALLCNAYSTNSECFTLPMGVLVETIYGNGSMRITLPGTNCMASGSVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSLALAPALVETYSRLLVYMEIESLGIKGFISQLLPNVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHSLAAVPQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMNFTPHNWASHTLSCFPAPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMTNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGFRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENAPEHWLQSDWHTKHMTYHKKYPEKLYFEGLAEQVNPPIQLQSQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYERHLRDRTNLKRKLVHAIMSSLKDNRTPGWCLSETYLKCGMNAREDNVWIPDDTYYCKLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLVITALPEPYWIVLHDRIVSVISSAVLSSGSDWLGYPFQLLDFTACHQSYSEMHCSYVLALAHAVWHHSSIGQLSLIPKFLSEVLKPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMLEIGVAFYEMLQAVDQHSNHLAYMDPICDFLYHIKYMFTGDSVKDQVERIICSLRPAMRLRLRFITHISKMEPAAVPVSNSSSVQQTSSASSPTAQSTAGAAIPLSVTQ

Target ORF information:

RefSeq Version	XM_005160334.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator complex subunit 23 (med23), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160334.4

ORF Insert Sequence:

ATGGCGGTCC CGATGGAGAC TCAGCTGCAG AGCATCTTTG AGGATGTAGT GAAAACGGAG 
GTGATCGAGG AGGCGTTTGC TGGCATGTTC ATGGACAGCC CTGAGGATGA GAAGACCAAA 
CTCACAAGCT GTTTAGGAGC CTTTAGGCAG TTCTGGAGTA CCCTGCCGCA GGAGTCTCAT 
GAGCAGTGTG TGCAGTGGAT CGTGCGCTTC ATTCATGGTC AGCACAGCCC CAAACGCATC 
GCCTTCCTCT ACGACTGTCT GGCCATGGCG GTGGAGACCA GCCTGCTGCC ACCCAGGATG 
GTGTGTCAGG CTCTCATCAG CTCCGACAGT CTGGAGTGGG AACGGACTCA ACTCTGGGCT 
TTGACCTTCA AACTCATCCG CAAAATCATC GGAGGAGTCG ACTATAAGGG TGTGAGAGAT 
CTATTGAAGG CCGTGTTGGA CAAGATTCAG ACCATCCCCA ACTTTGTGAG CTCAGCTGTG 
GTTCAGCAGC TGCTCGCGGC ACGAGAGGTT GTGGAGTATA TACTGGACCG CAACGCCTGT 
CTGCTGCCGG CGTACTTCGC TGTGACTGAG ATCCGGAAAC TTTGTCCAGA GGGGGCGCTC 
TCACACTGGC TGTTGGGCAG CCTGATATCT GACTTTGTCG ACAGTTTCAG ACCGACGGCA 
CGTATAAACT CCATATGTGG GCGCTGCAGT CTTCTGCCTG TGGTCAATAA TTCTGGGGCC 
ATCTGTAACT CATGGAAGCT GGACCCCACC ACTCTGCGTT TCCCTCTGAG AGGAATGCTG 
CCGTATGATA AGGACCTGTT TGATCCGCAG ACAGGGTTGC TGAGGTATGT GCTGGAGCAG 
CCGTACTCCA GAGACATGGT GTGCAACATG CTGGGCCTCA ACAAACAGCA CAAGCAGAGG 
TGTCCGGTTC TGGAGGACCA GCTGGTGGAT CTGGTGGTTT ATGCCATGGA GCGCTCTGAG 
ACTGAGGAGC AGTTTGATGA CGGCGGCACC AGCCAGCTCC TGTGGCAGCA TCTGTCCAGC 
CAGCTCATCT TCTTCGTCCT CTTCCAGTTT GCCAGCTTCC CTCACATGGT GCTGTCGCTC 
CATCAGAAGC TGGCAGGTCG GGGTTTGATC AAGGGCAGGG ATCATCTGAT GTGGGTTCTG 
CTGCAGTTCA TCTCCGGCAG CATTCAGAAG AACGCTCTGG GAGACTTTCT CCCCGTCATG 
AAGCTCTTCG ACCTGCTGTA CCCTGAGAAA GAGTGTATTC CAGTCCCGGA CATCAACAAG 
CCCCAGTCCA CACACGCATT CGCCATGACC TGCATCTGGA TTCACCTCAA CCGCAAAGCC 
CAGAACGACA ACTCCAAACT GCAGATCCCC ATCCCACACT CACTGAAGCT GCACCATGAG 
TTTCTCCAGC AGAGTCTGCG CAATAAGAGT TTGCCCATGA CAGACTATAA AATCGCTCTG 
CTGTGTAACG CCTACTCCAC CAACTCCGAG TGCTTCACGC TGCCCATGGG CGTCCTGGTG 
GAGACCATCT ACGGCAATGG TAGCATGCGC ATCACTCTGC CAGGCACCAA TTGCATGGCG 
TCCGGCTCTG TCACGCCACT GCCCATGAAC CTGCTGGACT CCCTAACCGT CCATGCTAAG 
ATGAGTTTGA TCCACAGCAT CGCCACCCGT GTTATTAAAC TAGCCCACGC TAAGTCCAGT 
CTGGCTCTGG CTCCTGCGCT GGTGGAGACG TACAGCCGGC TGCTGGTCTA CATGGAGATT 
GAGTCGCTCG GCATCAAGGG ATTCATCAGT CAGCTTCTGC CGAATGTGTT CAAGTCTCAC 
GCGTGGGGGA TTCTGCACAC ACTGCTGGAG ATGTTCAGCT ACCGCATGCA CCACATTCAG 
CCTCATTACC GCGTGCAGCT CCTGTCCCAT CTGCACTCGC TCGCCGCTGT CCCACAGACC 
AATCAGAACC AGCTGCACCT CTGTGTTGAA AGCACAGCTC TGCGCCTGAT CACGGCTCTG 
GGTAGTTCCG AGGTTCAGCC TCAGTTCACA CGGTTCCTCA ACGACCCAAA AACAGTGCTG 
TCGGCCGAGT CTGAGGAGCT AAACCGAGCA CTCATCCTCA CACTGGCCCG GGCCACTCAC 
GTCACAGATT TCTTTACAGG CTCGGACTCG ATTCAGGGCA CTTGGTGTAA AGACATCCTG 
CAGACCATCA TGAACTTCAC ACCTCATAAC TGGGCCTCTC ACACTCTTAG CTGTTTCCCT 
GCTCCACTGC AGGCGTTCTT CAAGCAGAAT AATGTCCCTC AGGAGAGCCG CTTCAACCTG 
AAGAAGAACG TGGAGGAGGA GTACAGGAAG TGGAAGTCCA TGACCAATGA GAACGACATC 
ATCACCCACT TCTCCATGCA GGGCTCGCCG CCGCTCTTCC TGTGTCTGCT GTGGAAGATG 
CTGCTGGAGA CGGACCACAT CAACCAAATC GGCTTCAGGG TTCTGGAGCG CATCGGGGCG 
CGAGCGCTGG TGGCTCATGT GCGCACGTTT GCCGACTTCC TGGTGTACGA GTTCTCCACA 
TCCGCTGGCG GACAGCAGCT AAACAAGTGC ATCGAGATCC TGAACGACAT GGTGTGGAAG 
TACAATATCG TGACCCTGGA TAGACTCATC CTCTGCCTGG CGATGAGGAG TCACGAAGGG 
AACGAGGCTC AGGTCTGCTA CTTCATCATC CAGCTTCTCC TGCTCAAACC CAACGACTTC 
CGCAACCGCG TCAGTGACTT TGTGAAGGAG AACGCTCCAG AACACTGGCT GCAGAGCGAC 
TGGCACACCA AACACATGAC CTACCACAAG AAGTATCCAG AGAAGCTGTA TTTCGAGGGT 
CTGGCGGAGC AGGTCAATCC TCCTATCCAG CTGCAGTCTC AGTATCTGCC CATTTACTTC 
GGCAACGTGT GTCTGCGCTT CCTCCCCGTG TTCGACATCG TCATCCACCG CTTCCTGGAG 
CTGCTGCCGG TCTCCAAATC CCTGGAGACG CTGCTCGATC ATCTGGGAGG ACTCTACAAG 
TTTCATGATC GGCCGGTGAC GTACCTGTAC AACACGCTGC ATTATTATGA GAGGCACCTA 
CGCGACCGCA CAAACCTGAA GAGGAAGCTG GTGCATGCCA TCATGAGCTC GCTGAAGGAC 
AATCGAACAC CCGGCTGGTG CCTTAGCGAG ACCTACCTGA AGTGCGGCAT GAACGCCCGT 
GAAGATAATG TCTGGATCCC TGACGACACC TACTACTGCA AACTCATCGG ACGCCTCGTG 
GATACCATGG CAGGTAAATC TCCAGGCCCG TTCCCCAACT GTGACTGGAG GTTTAACGAG 
TTCCCGAACC CGGCCGCTCA TGCGCTGCAC GTCACCTGTG TGGAGCTGAT GGCTCTGGCG 
GTCCCGGGGA AAGACGTGGG CAATGCTCTG CTTAATGTGG TGCTCAAGAG TCAGCCGCTG 
GTGCCTCGAG AGAACATCAC GGCCTGGATG AACGCCATCG GACTGGTCAT CACCGCTCTG 
CCTGAGCCCT ACTGGATCGT CCTGCATGAC CGTATCGTGA GTGTGATCAG CAGTGCGGTG 
CTCAGCTCTG GGTCAGATTG GCTGGGTTAC CCATTCCAGC TTCTGGACTT CACCGCTTGC 
CATCAGTCCT ACTCAGAGAT GCACTGCAGT TACGTTCTGG CTCTGGCTCA CGCCGTGTGG 
CATCACTCCA GCATCGGCCA GCTCTCGCTC ATACCCAAGT TCCTGTCGGA GGTCCTGAAG 
CCCATCGTGA AGACTGAGTT CCAGCTGCTG TACGTTTATC ATCTGGTGGG CCCGTTTCTG 
CAGCGCTTCC AGCAGGAGAG AACCAGATGC ATGCTGGAGA TTGGAGTGGC GTTTTATGAG 
ATGCTTCAGG CTGTGGATCA GCACAGCAAC CACCTGGCCT ACATGGACCC CATCTGTGAC 
TTCCTGTACC ACATCAAATA CATGTTTACT GGGGACAGTG TCAAAGACCA GGTGGAGCGC 
ATCATCTGTT CTCTGCGTCC AGCAATGCGT CTTCGTCTAC GCTTCATCAC ACACATCAGT 
AAGATGGAGC CGGCGGCGGT GCCCGTGTCC AACAGCAGCA GCGTCCAGCA GACCAGCAGC 
GCCTCCTCAC CCACAGCACA GAGCACTGCT GGAGCCGCCA TACCACTGTC TGTGACACAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med23 ( XM_005160335.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160392.1 Danio rerio mediator complex subunit 23 (med23), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa24591
Clone ID Related Accession (Same CDS sequence)	XM_005160335.3 , XM_005160335.4
Accession Version	XM_005160335.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mediator of RNA polymerase II transcription subunit 23 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160335.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTCC CGATGGAGAC TCAGCTGCAG AGCATCTTTG AGGATGTAGT GAAAACGGAG 
GTGATCGAGG AGGCGTTTGC TGGCATGTTC ATGGACAGCC CTGAGGATGA GAAGACCAAA 
CTCACAAGCT GTTTAGGAGC CTTTAGGCAG TTCTGGAGTA CCCTGCCGCA GGAGTCTCAT 
GAGCAGTGTG TGCAGTGGAT CGTGCGCTTC ATTCATGGTC AGCACAGCCC CAAACGCATC 
GCCTTCCTCT ACGACTGTCT GGCCATGGCG GTGGAGACCA GCCTGCTGCC ACCCAGGATG 
GTGTGTCAGG CTCTCATCAG CTCCGACAGT CTGGAGTGGG AACGGACTCA ACTCTGGGCT 
TTGACCTTCA AACTCATCCG CAAAATCATC GGAGGAGTCG ACTATAAGGG TGTGAGAGAT 
CTATTGAAGG CCGTGTTGGA CAAGATTCAG ACCATCCCCA ACTTTGTGAG CTCAGCTGTG 
GTTCAGCAGC TGCTCGCGGC ACGAGAGGTT GTGGAGTATA TACTGGACCG CAACGCCTGT 
CTGCTGCCGG CGTACTTCGC TGTGACTGAG ATCCGGAAAC TTTGTCCAGA GGGGGCGCTC 
TCACACTGGC TGTTGGGCAG CCTGATATCT GACTTTGTCG ACAGTTTCAG ACCGACGGCA 
CGTATAAACT CCATATGTGG GCGCTGCAGT CTTCTGCCTG TGGTCAATAA TTCTGGGGCC 
ATCTGTAACT CATGGAAGCT GGACCCCACC ACTCTGCGTT TCCCTCTGAG AGGAATGCTG 
CCGTATGATA AGGACCTGTT TGATCCGCAG ACAGGGTTGC TGAGGTATGT GCTGGAGCAG 
CCGTACTCCA GAGACATGGT GTGCAACATG CTGGGCCTCA ACAAACAGAC CTTGAACATT 
GCTCAGCACA AGCAGAGGTG TCCGGTTCTG GAGGACCAGC TGGTGGATCT GGTGGTTTAT 
GCCATGGAGC GCTCTGAGAC TGAGGAGCAG TTTGATGACG GCGGCACCAG CCAGCTCCTG 
TGGCAGCATC TGTCCAGCCA GCTCATCTTC TTCGTCCTCT TCCAGTTTGC CAGCTTCCCT 
CACATGGTGC TGTCGCTCCA TCAGAAGCTG GCAGGTCGGG GTTTGATCAA GGGCAGGGAT 
CATCTGATGT GGGTTCTGCT GCAGTTCATC TCCGGCAGCA TTCAGAAGAA CGCTCTGGGA 
GACTTTCTCC CCGTCATGAA GCTCTTCGAC CTGCTGTACC CTGAGAAAGA GTGTATTCCA 
GTCCCGGACA TCAACAAGCC CCAGTCCACA CACGCATTCG CCATGACCTG CATCTGGATT 
CACCTCAACC GCAAAGCCCA GAACGACAAC TCCAAACTGC AGATCCCCAT CCCACACTCA 
CTGAAGCTGC ACCATGAGTT TCTCCAGCAG AGTCTGCGCA ATAAGAGTTT GCCCATGACA 
GACTATAAAA TCGCTCTGCT GTGTAACGCC TACTCCACCA ACTCCGAGTG CTTCACGCTG 
CCCATGGGCG TCCTGGTGGA GACCATCTAC GGCAATGGTA GCATGCGCAT CACTCTGCCA 
GGCACCAATT GCATGGCGTC CGGCTCTGTC ACGCCACTGC CCATGAACCT GCTGGACTCC 
CTAACCGTCC ATGCTAAGAT GAGTTTGATC CACAGCATCG CCACCCGTGT TATTAAACTA 
GCCCACGCTA AGTCCAGTCT GGCTCTGGCT CCTGCGCTGG TGGAGACGTA CAGCCGGCTG 
CTGGTCTACA TGGAGATTGA GTCGCTCGGC ATCAAGGGAT TCATCAGTCA GCTTCTGCCG 
AATGTGTTCA AGTCTCACGC GTGGGGGATT CTGCACACAC TGCTGGAGAT GTTCAGCTAC 
CGCATGCACC ACATTCAGCC TCATTACCGC GTGCAGCTCC TGTCCCATCT GCACTCGCTC 
GCCGCTGTCC CACAGACCAA TCAGAACCAG CTGCACCTCT GTGTTGAAAG CACAGCTCTG 
CGCCTGATCA CGGCTCTGGG TAGTTCCGAG GTTCAGCCTC AGTTCACACG GTTCCTCAAC 
GACCCAAAAA CAGTGCTGTC GGCCGAGTCT GAGGAGCTAA ACCGAGCACT CATCCTCACA 
CTGGCCCGGG CCACTCACGT CACAGATTTC TTTACAGGCT CGGACTCGAT TCAGGGCACT 
TGGTGTAAAG ACATCCTGCA GACCATCATG AACTTCACAC CTCATAACTG GGCCTCTCAC 
ACTCTTAGCT GTTTCCCTGC TCCACTGCAG GCGTTCTTCA AGCAGAATAA TGTCCCTCAG 
GAGAGCCGCT TCAACCTGAA GAAGAACGTG GAGGAGGAGT ACAGGAAGTG GAAGTCCATG 
ACCAATGAGA ACGACATCAT CACCCACTTC TCCATGCAGG GCTCGCCGCC GCTCTTCCTG 
TGTCTGCTGT GGAAGATGCT GCTGGAGACG GACCACATCA ACCAAATCGG CTTCAGGGTT 
CTGGAGCGCA TCGGGGCGCG AGCGCTGGTG GCTCATGTGC GCACGTTTGC CGACTTCCTG 
GTGTACGAGT TCTCCACATC CGCTGGCGGA CAGCAGCTAA ACAAGTGCAT CGAGATCCTG 
AACGACATGG TGTGGAAGTA CAATATCGTG ACCCTGGATA GACTCATCCT CTGCCTGGCG 
ATGAGGAGTC ACGAAGGGAA CGAGGCTCAG GTCTGCTACT TCATCATCCA GCTTCTCCTG 
CTCAAACCCA ACGACTTCCG CAACCGCGTC AGTGACTTTG TGAAGGAGAA CGCTCCAGAA 
CACTGGCTGC AGAGCGACTG GCACACCAAA CACATGACCT ACCACAAGAA GTATCCAGAG 
AAGCTGTATT TCGAGGGTCT GGCGGAGCAG GTCAATCCTC CTATCCAGCT GCAGTCTCAG 
TATCTGCCCA TTTACTTCGG CAACGTGTGT CTGCGCTTCC TCCCCGTGTT CGACATCGTC 
ATCCACCGCT TCCTGGAGCT GCTGCCGGTC TCCAAATCCC TGGAGACGCT GCTCGATCAT 
CTGGGAGGAC TCTACAAGTT TCATGATCGG CCGGTGACGT ACCTGTACAA CACGCTGCAT 
TATTATGAGA GGCACCTACG CGACCGCACA AACCTGAAGA GGAAGCTGGT GCATGCCATC 
ATGAGCTCGC TGAAGGACAA TCGAACACCC GGCTGGTGCC TTAGCGAGAC CTACCTGAAG 
TGCGGCATGA ACGCCCGTGA AGATAATGTC TGGATCCCTG ACGACACCTA CTACTGCAAA 
CTCATCGGAC GCCTCGTGGA TACCATGGCA GGTAAATCTC CAGGCCCGTT CCCCAACTGT 
GACTGGAGGT TTAACGAGTT CCCGAACCCG GCCGCTCATG CGCTGCACGT CACCTGTGTG 
GAGCTGATGG CTCTGGCGGT CCCGGGGAAA GACGTGGGCA ATGCTCTGCT TAATGTGGTG 
CTCAAGAGTC AGCCGCTGGT GCCTCGAGAG AACATCACGG CCTGGATGAA CGCCATCGGA 
CTGGTCATCA CCGCTCTGCC TGAGCCCTAC TGGATCGTCC TGCATGACCG TATCGTGAGT 
GTGATCAGCA GTGCGGTGCT CAGCTCTGGG TCAGATTGGC TGGGTTACCC ATTCCAGCTT 
CTGGACTTCA CCGCTTGCCA TCAGTCCTAC TCAGAGATGC ACTGCAGTTA CGTTCTGGCT 
CTGGCTCACG CCGTGTGGCA TCACTCCAGC ATCGGCCAGC TCTCGCTCAT ACCCAAGTTC 
CTGTCGGAGG TCCTGAAGCC CATCGTGAAG ACTGAGTTCC AGCTGCTGTA CGTTTATCAT 
CTGGTGGGCC CGTTTCTGCA GCGCTTCCAG CAGGAGAGAA CCAGATGCAT GCTGGAGATT 
GGAGTGGCGT TTTATGAGAT GCTTCAGGCT GTGGATCAGC ACAGCAACCA CCTGGCCTAC 
ATGGACCCCA TCTGTGACTT CCTGTACCAC ATCAAATACA TGTTTACTGG GGACAGTGTC 
AAAGACCAGG TGGAGCGCAT CATCTGTTCT CTGCGTCCAG CAATGCGTCT TCGTCTACGC 
TTCATCACAC ACATCAGTAA GATGGAGCCG GCGGCGGTGC CCGTGTCCAA CAGCAGCAGC 
GTCCAGCAGA CCAGCAGCGC CTCCTCACCC ACAGCACAGA GCACTGCTGG AGCCGCCATA 
CCACTGTCTG TGACACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160392.1
CDS	159..4319
Translation	MAVPMETQLQSIFEDVVKTEVIEEAFAGMFMDSPEDEKTKLTSCLGAFRQFWSTLPQESHEQCVQWIVRFIHGQHSPKRIAFLYDCLAMAVETSLLPPRMVCQALISSDSLEWERTQLWALTFKLIRKIIGGVDYKGVRDLLKAVLDKIQTIPNFVSSAVVQQLLAAREVVEYILDRNACLLPAYFAVTEIRKLCPEGALSHWLLGSLISDFVDSFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPTTLRFPLRGMLPYDKDLFDPQTGLLRYVLEQPYSRDMVCNMLGLNKQTLNIAQHKQRCPVLEDQLVDLVVYAMERSETEEQFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALGDFLPVMKLFDLLYPEKECIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLRNKSLPMTDYKIALLCNAYSTNSECFTLPMGVLVETIYGNGSMRITLPGTNCMASGSVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSLALAPALVETYSRLLVYMEIESLGIKGFISQLLPNVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHSLAAVPQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMNFTPHNWASHTLSCFPAPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMTNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGFRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENAPEHWLQSDWHTKHMTYHKKYPEKLYFEGLAEQVNPPIQLQSQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYERHLRDRTNLKRKLVHAIMSSLKDNRTPGWCLSETYLKCGMNAREDNVWIPDDTYYCKLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLVITALPEPYWIVLHDRIVSVISSAVLSSGSDWLGYPFQLLDFTACHQSYSEMHCSYVLALAHAVWHHSSIGQLSLIPKFLSEVLKPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMLEIGVAFYEMLQAVDQHSNHLAYMDPICDFLYHIKYMFTGDSVKDQVERIICSLRPAMRLRLRFITHISKMEPAAVPVSNSSSVQQTSSASSPTAQSTAGAAIPLSVTQ

Target ORF information:

RefSeq Version	XM_005160335.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mediator complex subunit 23 (med23), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160335.3

ORF Insert Sequence:

ATGGCGGTCC CGATGGAGAC TCAGCTGCAG AGCATCTTTG AGGATGTAGT GAAAACGGAG 
GTGATCGAGG AGGCGTTTGC TGGCATGTTC ATGGACAGCC CTGAGGATGA GAAGACCAAA 
CTCACAAGCT GTTTAGGAGC CTTTAGGCAG TTCTGGAGTA CCCTGCCGCA GGAGTCTCAT 
GAGCAGTGTG TGCAGTGGAT CGTGCGCTTC ATTCATGGTC AGCACAGCCC CAAACGCATC 
GCCTTCCTCT ACGACTGTCT GGCCATGGCG GTGGAGACCA GCCTGCTGCC ACCCAGGATG 
GTGTGTCAGG CTCTCATCAG CTCCGACAGT CTGGAGTGGG AACGGACTCA ACTCTGGGCT 
TTGACCTTCA AACTCATCCG CAAAATCATC GGAGGAGTCG ACTATAAGGG TGTGAGAGAT 
CTATTGAAGG CCGTGTTGGA CAAGATTCAG ACCATCCCCA ACTTTGTGAG CTCAGCTGTG 
GTTCAGCAGC TGCTCGCGGC ACGAGAGGTT GTGGAGTATA TACTGGACCG CAACGCCTGT 
CTGCTGCCGG CGTACTTCGC TGTGACTGAG ATCCGGAAAC TTTGTCCAGA GGGGGCGCTC 
TCACACTGGC TGTTGGGCAG CCTGATATCT GACTTTGTCG ACAGTTTCAG ACCGACGGCA 
CGTATAAACT CCATATGTGG GCGCTGCAGT CTTCTGCCTG TGGTCAATAA TTCTGGGGCC 
ATCTGTAACT CATGGAAGCT GGACCCCACC ACTCTGCGTT TCCCTCTGAG AGGAATGCTG 
CCGTATGATA AGGACCTGTT TGATCCGCAG ACAGGGTTGC TGAGGTATGT GCTGGAGCAG 
CCGTACTCCA GAGACATGGT GTGCAACATG CTGGGCCTCA ACAAACAGAC CTTGAACATT 
GCTCAGCACA AGCAGAGGTG TCCGGTTCTG GAGGACCAGC TGGTGGATCT GGTGGTTTAT 
GCCATGGAGC GCTCTGAGAC TGAGGAGCAG TTTGATGACG GCGGCACCAG CCAGCTCCTG 
TGGCAGCATC TGTCCAGCCA GCTCATCTTC TTCGTCCTCT TCCAGTTTGC CAGCTTCCCT 
CACATGGTGC TGTCGCTCCA TCAGAAGCTG GCAGGTCGGG GTTTGATCAA GGGCAGGGAT 
CATCTGATGT GGGTTCTGCT GCAGTTCATC TCCGGCAGCA TTCAGAAGAA CGCTCTGGGA 
GACTTTCTCC CCGTCATGAA GCTCTTCGAC CTGCTGTACC CTGAGAAAGA GTGTATTCCA 
GTCCCGGACA TCAACAAGCC CCAGTCCACA CACGCATTCG CCATGACCTG CATCTGGATT 
CACCTCAACC GCAAAGCCCA GAACGACAAC TCCAAACTGC AGATCCCCAT CCCACACTCA 
CTGAAGCTGC ACCATGAGTT TCTCCAGCAG AGTCTGCGCA ATAAGAGTTT GCCCATGACA 
GACTATAAAA TCGCTCTGCT GTGTAACGCC TACTCCACCA ACTCCGAGTG CTTCACGCTG 
CCCATGGGCG TCCTGGTGGA GACCATCTAC GGCAATGGTA GCATGCGCAT CACTCTGCCA 
GGCACCAATT GCATGGCGTC CGGCTCTGTC ACGCCACTGC CCATGAACCT GCTGGACTCC 
CTAACCGTCC ATGCTAAGAT GAGTTTGATC CACAGCATCG CCACCCGTGT TATTAAACTA 
GCCCACGCTA AGTCCAGTCT GGCTCTGGCT CCTGCGCTGG TGGAGACGTA CAGCCGGCTG 
CTGGTCTACA TGGAGATTGA GTCGCTCGGC ATCAAGGGAT TCATCAGTCA GCTTCTGCCG 
AATGTGTTCA AGTCTCACGC GTGGGGGATT CTGCACACAC TGCTGGAGAT GTTCAGCTAC 
CGCATGCACC ACATTCAGCC TCATTACCGC GTGCAGCTCC TGTCCCATCT GCACTCGCTC 
GCCGCTGTCC CACAGACCAA TCAGAACCAG CTGCACCTCT GTGTTGAAAG CACAGCTCTG 
CGCCTGATCA CGGCTCTGGG TAGTTCCGAG GTTCAGCCTC AGTTCACACG GTTCCTCAAC 
GACCCAAAAA CAGTGCTGTC GGCCGAGTCT GAGGAGCTAA ACCGAGCACT CATCCTCACA 
CTGGCCCGGG CCACTCACGT CACAGATTTC TTTACAGGCT CGGACTCGAT TCAGGGCACT 
TGGTGTAAAG ACATCCTGCA GACCATCATG AACTTCACAC CTCATAACTG GGCCTCTCAC 
ACTCTTAGCT GTTTCCCTGC TCCACTGCAG GCGTTCTTCA AGCAGAATAA TGTCCCTCAG 
GAGAGCCGCT TCAACCTGAA GAAGAACGTG GAGGAGGAGT ACAGGAAGTG GAAGTCCATG 
ACCAATGAGA ACGACATCAT CACCCACTTC TCCATGCAGG GCTCGCCGCC GCTCTTCCTG 
TGTCTGCTGT GGAAGATGCT GCTGGAGACG GACCACATCA ACCAAATCGG CTTCAGGGTT 
CTGGAGCGCA TCGGGGCGCG AGCGCTGGTG GCTCATGTGC GCACGTTTGC CGACTTCCTG 
GTGTACGAGT TCTCCACATC CGCTGGCGGA CAGCAGCTAA ACAAGTGCAT CGAGATCCTG 
AACGACATGG TGTGGAAGTA CAATATCGTG ACCCTGGATA GACTCATCCT CTGCCTGGCG 
ATGAGGAGTC ACGAAGGGAA CGAGGCTCAG GTCTGCTACT TCATCATCCA GCTTCTCCTG 
CTCAAACCCA ACGACTTCCG CAACCGCGTC AGTGACTTTG TGAAGGAGAA CGCTCCAGAA 
CACTGGCTGC AGAGCGACTG GCACACCAAA CACATGACCT ACCACAAGAA GTATCCAGAG 
AAGCTGTATT TCGAGGGTCT GGCGGAGCAG GTCAATCCTC CTATCCAGCT GCAGTCTCAG 
TATCTGCCCA TTTACTTCGG CAACGTGTGT CTGCGCTTCC TCCCCGTGTT CGACATCGTC 
ATCCACCGCT TCCTGGAGCT GCTGCCGGTC TCCAAATCCC TGGAGACGCT GCTCGATCAT 
CTGGGAGGAC TCTACAAGTT TCATGATCGG CCGGTGACGT ACCTGTACAA CACGCTGCAT 
TATTATGAGA GGCACCTACG CGACCGCACA AACCTGAAGA GGAAGCTGGT GCATGCCATC 
ATGAGCTCGC TGAAGGACAA TCGAACACCC GGCTGGTGCC TTAGCGAGAC CTACCTGAAG 
TGCGGCATGA ACGCCCGTGA AGATAATGTC TGGATCCCTG ACGACACCTA CTACTGCAAA 
CTCATCGGAC GCCTCGTGGA TACCATGGCA GGTAAATCTC CAGGCCCGTT CCCCAACTGT 
GACTGGAGGT TTAACGAGTT CCCGAACCCG GCCGCTCATG CGCTGCACGT CACCTGTGTG 
GAGCTGATGG CTCTGGCGGT CCCGGGGAAA GACGTGGGCA ATGCTCTGCT TAATGTGGTG 
CTCAAGAGTC AGCCGCTGGT GCCTCGAGAG AACATCACGG CCTGGATGAA CGCCATCGGA 
CTGGTCATCA CCGCTCTGCC TGAGCCCTAC TGGATCGTCC TGCATGACCG TATCGTGAGT 
GTGATCAGCA GTGCGGTGCT CAGCTCTGGG TCAGATTGGC TGGGTTACCC ATTCCAGCTT 
CTGGACTTCA CCGCTTGCCA TCAGTCCTAC TCAGAGATGC ACTGCAGTTA CGTTCTGGCT 
CTGGCTCACG CCGTGTGGCA TCACTCCAGC ATCGGCCAGC TCTCGCTCAT ACCCAAGTTC 
CTGTCGGAGG TCCTGAAGCC CATCGTGAAG ACTGAGTTCC AGCTGCTGTA CGTTTATCAT 
CTGGTGGGCC CGTTTCTGCA GCGCTTCCAG CAGGAGAGAA CCAGATGCAT GCTGGAGATT 
GGAGTGGCGT TTTATGAGAT GCTTCAGGCT GTGGATCAGC ACAGCAACCA CCTGGCCTAC 
ATGGACCCCA TCTGTGACTT CCTGTACCAC ATCAAATACA TGTTTACTGG GGACAGTGTC 
AAAGACCAGG TGGAGCGCAT CATCTGTTCT CTGCGTCCAG CAATGCGTCT TCGTCTACGC 
TTCATCACAC ACATCAGTAA GATGGAGCCG GCGGCGGTGC CCGTGTCCAA CAGCAGCAGC 
GTCCAGCAGA CCAGCAGCGC CTCCTCACCC ACAGCACAGA GCACTGCTGG AGCCGCCATA 
CCACTGTCTG TGACACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med23 ( XM_005160335.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160392.1 Danio rerio mediator complex subunit 23 (med23), transcript variant X2, mRNA.

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CloneID	ODa24591
Clone ID Related Accession (Same CDS sequence)	XM_005160335.3 , XM_005160335.4
Accession Version	XM_005160335.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mediator of RNA polymerase II transcription subunit 23 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160335.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTCC CGATGGAGAC TCAGCTGCAG AGCATCTTTG AGGATGTAGT GAAAACGGAG 
GTGATCGAGG AGGCGTTTGC TGGCATGTTC ATGGACAGCC CTGAGGATGA GAAGACCAAA 
CTCACAAGCT GTTTAGGAGC CTTTAGGCAG TTCTGGAGTA CCCTGCCGCA GGAGTCTCAT 
GAGCAGTGTG TGCAGTGGAT CGTGCGCTTC ATTCATGGTC AGCACAGCCC CAAACGCATC 
GCCTTCCTCT ACGACTGTCT GGCCATGGCG GTGGAGACCA GCCTGCTGCC ACCCAGGATG 
GTGTGTCAGG CTCTCATCAG CTCCGACAGT CTGGAGTGGG AACGGACTCA ACTCTGGGCT 
TTGACCTTCA AACTCATCCG CAAAATCATC GGAGGAGTCG ACTATAAGGG TGTGAGAGAT 
CTATTGAAGG CCGTGTTGGA CAAGATTCAG ACCATCCCCA ACTTTGTGAG CTCAGCTGTG 
GTTCAGCAGC TGCTCGCGGC ACGAGAGGTT GTGGAGTATA TACTGGACCG CAACGCCTGT 
CTGCTGCCGG CGTACTTCGC TGTGACTGAG ATCCGGAAAC TTTGTCCAGA GGGGGCGCTC 
TCACACTGGC TGTTGGGCAG CCTGATATCT GACTTTGTCG ACAGTTTCAG ACCGACGGCA 
CGTATAAACT CCATATGTGG GCGCTGCAGT CTTCTGCCTG TGGTCAATAA TTCTGGGGCC 
ATCTGTAACT CATGGAAGCT GGACCCCACC ACTCTGCGTT TCCCTCTGAG AGGAATGCTG 
CCGTATGATA AGGACCTGTT TGATCCGCAG ACAGGGTTGC TGAGGTATGT GCTGGAGCAG 
CCGTACTCCA GAGACATGGT GTGCAACATG CTGGGCCTCA ACAAACAGAC CTTGAACATT 
GCTCAGCACA AGCAGAGGTG TCCGGTTCTG GAGGACCAGC TGGTGGATCT GGTGGTTTAT 
GCCATGGAGC GCTCTGAGAC TGAGGAGCAG TTTGATGACG GCGGCACCAG CCAGCTCCTG 
TGGCAGCATC TGTCCAGCCA GCTCATCTTC TTCGTCCTCT TCCAGTTTGC CAGCTTCCCT 
CACATGGTGC TGTCGCTCCA TCAGAAGCTG GCAGGTCGGG GTTTGATCAA GGGCAGGGAT 
CATCTGATGT GGGTTCTGCT GCAGTTCATC TCCGGCAGCA TTCAGAAGAA CGCTCTGGGA 
GACTTTCTCC CCGTCATGAA GCTCTTCGAC CTGCTGTACC CTGAGAAAGA GTGTATTCCA 
GTCCCGGACA TCAACAAGCC CCAGTCCACA CACGCATTCG CCATGACCTG CATCTGGATT 
CACCTCAACC GCAAAGCCCA GAACGACAAC TCCAAACTGC AGATCCCCAT CCCACACTCA 
CTGAAGCTGC ACCATGAGTT TCTCCAGCAG AGTCTGCGCA ATAAGAGTTT GCCCATGACA 
GACTATAAAA TCGCTCTGCT GTGTAACGCC TACTCCACCA ACTCCGAGTG CTTCACGCTG 
CCCATGGGCG TCCTGGTGGA GACCATCTAC GGCAATGGTA GCATGCGCAT CACTCTGCCA 
GGCACCAATT GCATGGCGTC CGGCTCTGTC ACGCCACTGC CCATGAACCT GCTGGACTCC 
CTAACCGTCC ATGCTAAGAT GAGTTTGATC CACAGCATCG CCACCCGTGT TATTAAACTA 
GCCCACGCTA AGTCCAGTCT GGCTCTGGCT CCTGCGCTGG TGGAGACGTA CAGCCGGCTG 
CTGGTCTACA TGGAGATTGA GTCGCTCGGC ATCAAGGGAT TCATCAGTCA GCTTCTGCCG 
AATGTGTTCA AGTCTCACGC GTGGGGGATT CTGCACACAC TGCTGGAGAT GTTCAGCTAC 
CGCATGCACC ACATTCAGCC TCATTACCGC GTGCAGCTCC TGTCCCATCT GCACTCGCTC 
GCCGCTGTCC CACAGACCAA TCAGAACCAG CTGCACCTCT GTGTTGAAAG CACAGCTCTG 
CGCCTGATCA CGGCTCTGGG TAGTTCCGAG GTTCAGCCTC AGTTCACACG GTTCCTCAAC 
GACCCAAAAA CAGTGCTGTC GGCCGAGTCT GAGGAGCTAA ACCGAGCACT CATCCTCACA 
CTGGCCCGGG CCACTCACGT CACAGATTTC TTTACAGGCT CGGACTCGAT TCAGGGCACT 
TGGTGTAAAG ACATCCTGCA GACCATCATG AACTTCACAC CTCATAACTG GGCCTCTCAC 
ACTCTTAGCT GTTTCCCTGC TCCACTGCAG GCGTTCTTCA AGCAGAATAA TGTCCCTCAG 
GAGAGCCGCT TCAACCTGAA GAAGAACGTG GAGGAGGAGT ACAGGAAGTG GAAGTCCATG 
ACCAATGAGA ACGACATCAT CACCCACTTC TCCATGCAGG GCTCGCCGCC GCTCTTCCTG 
TGTCTGCTGT GGAAGATGCT GCTGGAGACG GACCACATCA ACCAAATCGG CTTCAGGGTT 
CTGGAGCGCA TCGGGGCGCG AGCGCTGGTG GCTCATGTGC GCACGTTTGC CGACTTCCTG 
GTGTACGAGT TCTCCACATC CGCTGGCGGA CAGCAGCTAA ACAAGTGCAT CGAGATCCTG 
AACGACATGG TGTGGAAGTA CAATATCGTG ACCCTGGATA GACTCATCCT CTGCCTGGCG 
ATGAGGAGTC ACGAAGGGAA CGAGGCTCAG GTCTGCTACT TCATCATCCA GCTTCTCCTG 
CTCAAACCCA ACGACTTCCG CAACCGCGTC AGTGACTTTG TGAAGGAGAA CGCTCCAGAA 
CACTGGCTGC AGAGCGACTG GCACACCAAA CACATGACCT ACCACAAGAA GTATCCAGAG 
AAGCTGTATT TCGAGGGTCT GGCGGAGCAG GTCAATCCTC CTATCCAGCT GCAGTCTCAG 
TATCTGCCCA TTTACTTCGG CAACGTGTGT CTGCGCTTCC TCCCCGTGTT CGACATCGTC 
ATCCACCGCT TCCTGGAGCT GCTGCCGGTC TCCAAATCCC TGGAGACGCT GCTCGATCAT 
CTGGGAGGAC TCTACAAGTT TCATGATCGG CCGGTGACGT ACCTGTACAA CACGCTGCAT 
TATTATGAGA GGCACCTACG CGACCGCACA AACCTGAAGA GGAAGCTGGT GCATGCCATC 
ATGAGCTCGC TGAAGGACAA TCGAACACCC GGCTGGTGCC TTAGCGAGAC CTACCTGAAG 
TGCGGCATGA ACGCCCGTGA AGATAATGTC TGGATCCCTG ACGACACCTA CTACTGCAAA 
CTCATCGGAC GCCTCGTGGA TACCATGGCA GGTAAATCTC CAGGCCCGTT CCCCAACTGT 
GACTGGAGGT TTAACGAGTT CCCGAACCCG GCCGCTCATG CGCTGCACGT CACCTGTGTG 
GAGCTGATGG CTCTGGCGGT CCCGGGGAAA GACGTGGGCA ATGCTCTGCT TAATGTGGTG 
CTCAAGAGTC AGCCGCTGGT GCCTCGAGAG AACATCACGG CCTGGATGAA CGCCATCGGA 
CTGGTCATCA CCGCTCTGCC TGAGCCCTAC TGGATCGTCC TGCATGACCG TATCGTGAGT 
GTGATCAGCA GTGCGGTGCT CAGCTCTGGG TCAGATTGGC TGGGTTACCC ATTCCAGCTT 
CTGGACTTCA CCGCTTGCCA TCAGTCCTAC TCAGAGATGC ACTGCAGTTA CGTTCTGGCT 
CTGGCTCACG CCGTGTGGCA TCACTCCAGC ATCGGCCAGC TCTCGCTCAT ACCCAAGTTC 
CTGTCGGAGG TCCTGAAGCC CATCGTGAAG ACTGAGTTCC AGCTGCTGTA CGTTTATCAT 
CTGGTGGGCC CGTTTCTGCA GCGCTTCCAG CAGGAGAGAA CCAGATGCAT GCTGGAGATT 
GGAGTGGCGT TTTATGAGAT GCTTCAGGCT GTGGATCAGC ACAGCAACCA CCTGGCCTAC 
ATGGACCCCA TCTGTGACTT CCTGTACCAC ATCAAATACA TGTTTACTGG GGACAGTGTC 
AAAGACCAGG TGGAGCGCAT CATCTGTTCT CTGCGTCCAG CAATGCGTCT TCGTCTACGC 
TTCATCACAC ACATCAGTAA GATGGAGCCG GCGGCGGTGC CCGTGTCCAA CAGCAGCAGC 
GTCCAGCAGA CCAGCAGCGC CTCCTCACCC ACAGCACAGA GCACTGCTGG AGCCGCCATA 
CCACTGTCTG TGACACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160392.1
CDS	160..4320
Translation	MAVPMETQLQSIFEDVVKTEVIEEAFAGMFMDSPEDEKTKLTSCLGAFRQFWSTLPQESHEQCVQWIVRFIHGQHSPKRIAFLYDCLAMAVETSLLPPRMVCQALISSDSLEWERTQLWALTFKLIRKIIGGVDYKGVRDLLKAVLDKIQTIPNFVSSAVVQQLLAAREVVEYILDRNACLLPAYFAVTEIRKLCPEGALSHWLLGSLISDFVDSFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPTTLRFPLRGMLPYDKDLFDPQTGLLRYVLEQPYSRDMVCNMLGLNKQTLNIAQHKQRCPVLEDQLVDLVVYAMERSETEEQFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALGDFLPVMKLFDLLYPEKECIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLRNKSLPMTDYKIALLCNAYSTNSECFTLPMGVLVETIYGNGSMRITLPGTNCMASGSVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSLALAPALVETYSRLLVYMEIESLGIKGFISQLLPNVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHSLAAVPQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMNFTPHNWASHTLSCFPAPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMTNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGFRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENAPEHWLQSDWHTKHMTYHKKYPEKLYFEGLAEQVNPPIQLQSQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYERHLRDRTNLKRKLVHAIMSSLKDNRTPGWCLSETYLKCGMNAREDNVWIPDDTYYCKLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLVITALPEPYWIVLHDRIVSVISSAVLSSGSDWLGYPFQLLDFTACHQSYSEMHCSYVLALAHAVWHHSSIGQLSLIPKFLSEVLKPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMLEIGVAFYEMLQAVDQHSNHLAYMDPICDFLYHIKYMFTGDSVKDQVERIICSLRPAMRLRLRFITHISKMEPAAVPVSNSSSVQQTSSASSPTAQSTAGAAIPLSVTQ

Target ORF information:

RefSeq Version	XM_005160335.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator complex subunit 23 (med23), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160335.4

ORF Insert Sequence:

ATGGCGGTCC CGATGGAGAC TCAGCTGCAG AGCATCTTTG AGGATGTAGT GAAAACGGAG 
GTGATCGAGG AGGCGTTTGC TGGCATGTTC ATGGACAGCC CTGAGGATGA GAAGACCAAA 
CTCACAAGCT GTTTAGGAGC CTTTAGGCAG TTCTGGAGTA CCCTGCCGCA GGAGTCTCAT 
GAGCAGTGTG TGCAGTGGAT CGTGCGCTTC ATTCATGGTC AGCACAGCCC CAAACGCATC 
GCCTTCCTCT ACGACTGTCT GGCCATGGCG GTGGAGACCA GCCTGCTGCC ACCCAGGATG 
GTGTGTCAGG CTCTCATCAG CTCCGACAGT CTGGAGTGGG AACGGACTCA ACTCTGGGCT 
TTGACCTTCA AACTCATCCG CAAAATCATC GGAGGAGTCG ACTATAAGGG TGTGAGAGAT 
CTATTGAAGG CCGTGTTGGA CAAGATTCAG ACCATCCCCA ACTTTGTGAG CTCAGCTGTG 
GTTCAGCAGC TGCTCGCGGC ACGAGAGGTT GTGGAGTATA TACTGGACCG CAACGCCTGT 
CTGCTGCCGG CGTACTTCGC TGTGACTGAG ATCCGGAAAC TTTGTCCAGA GGGGGCGCTC 
TCACACTGGC TGTTGGGCAG CCTGATATCT GACTTTGTCG ACAGTTTCAG ACCGACGGCA 
CGTATAAACT CCATATGTGG GCGCTGCAGT CTTCTGCCTG TGGTCAATAA TTCTGGGGCC 
ATCTGTAACT CATGGAAGCT GGACCCCACC ACTCTGCGTT TCCCTCTGAG AGGAATGCTG 
CCGTATGATA AGGACCTGTT TGATCCGCAG ACAGGGTTGC TGAGGTATGT GCTGGAGCAG 
CCGTACTCCA GAGACATGGT GTGCAACATG CTGGGCCTCA ACAAACAGAC CTTGAACATT 
GCTCAGCACA AGCAGAGGTG TCCGGTTCTG GAGGACCAGC TGGTGGATCT GGTGGTTTAT 
GCCATGGAGC GCTCTGAGAC TGAGGAGCAG TTTGATGACG GCGGCACCAG CCAGCTCCTG 
TGGCAGCATC TGTCCAGCCA GCTCATCTTC TTCGTCCTCT TCCAGTTTGC CAGCTTCCCT 
CACATGGTGC TGTCGCTCCA TCAGAAGCTG GCAGGTCGGG GTTTGATCAA GGGCAGGGAT 
CATCTGATGT GGGTTCTGCT GCAGTTCATC TCCGGCAGCA TTCAGAAGAA CGCTCTGGGA 
GACTTTCTCC CCGTCATGAA GCTCTTCGAC CTGCTGTACC CTGAGAAAGA GTGTATTCCA 
GTCCCGGACA TCAACAAGCC CCAGTCCACA CACGCATTCG CCATGACCTG CATCTGGATT 
CACCTCAACC GCAAAGCCCA GAACGACAAC TCCAAACTGC AGATCCCCAT CCCACACTCA 
CTGAAGCTGC ACCATGAGTT TCTCCAGCAG AGTCTGCGCA ATAAGAGTTT GCCCATGACA 
GACTATAAAA TCGCTCTGCT GTGTAACGCC TACTCCACCA ACTCCGAGTG CTTCACGCTG 
CCCATGGGCG TCCTGGTGGA GACCATCTAC GGCAATGGTA GCATGCGCAT CACTCTGCCA 
GGCACCAATT GCATGGCGTC CGGCTCTGTC ACGCCACTGC CCATGAACCT GCTGGACTCC 
CTAACCGTCC ATGCTAAGAT GAGTTTGATC CACAGCATCG CCACCCGTGT TATTAAACTA 
GCCCACGCTA AGTCCAGTCT GGCTCTGGCT CCTGCGCTGG TGGAGACGTA CAGCCGGCTG 
CTGGTCTACA TGGAGATTGA GTCGCTCGGC ATCAAGGGAT TCATCAGTCA GCTTCTGCCG 
AATGTGTTCA AGTCTCACGC GTGGGGGATT CTGCACACAC TGCTGGAGAT GTTCAGCTAC 
CGCATGCACC ACATTCAGCC TCATTACCGC GTGCAGCTCC TGTCCCATCT GCACTCGCTC 
GCCGCTGTCC CACAGACCAA TCAGAACCAG CTGCACCTCT GTGTTGAAAG CACAGCTCTG 
CGCCTGATCA CGGCTCTGGG TAGTTCCGAG GTTCAGCCTC AGTTCACACG GTTCCTCAAC 
GACCCAAAAA CAGTGCTGTC GGCCGAGTCT GAGGAGCTAA ACCGAGCACT CATCCTCACA 
CTGGCCCGGG CCACTCACGT CACAGATTTC TTTACAGGCT CGGACTCGAT TCAGGGCACT 
TGGTGTAAAG ACATCCTGCA GACCATCATG AACTTCACAC CTCATAACTG GGCCTCTCAC 
ACTCTTAGCT GTTTCCCTGC TCCACTGCAG GCGTTCTTCA AGCAGAATAA TGTCCCTCAG 
GAGAGCCGCT TCAACCTGAA GAAGAACGTG GAGGAGGAGT ACAGGAAGTG GAAGTCCATG 
ACCAATGAGA ACGACATCAT CACCCACTTC TCCATGCAGG GCTCGCCGCC GCTCTTCCTG 
TGTCTGCTGT GGAAGATGCT GCTGGAGACG GACCACATCA ACCAAATCGG CTTCAGGGTT 
CTGGAGCGCA TCGGGGCGCG AGCGCTGGTG GCTCATGTGC GCACGTTTGC CGACTTCCTG 
GTGTACGAGT TCTCCACATC CGCTGGCGGA CAGCAGCTAA ACAAGTGCAT CGAGATCCTG 
AACGACATGG TGTGGAAGTA CAATATCGTG ACCCTGGATA GACTCATCCT CTGCCTGGCG 
ATGAGGAGTC ACGAAGGGAA CGAGGCTCAG GTCTGCTACT TCATCATCCA GCTTCTCCTG 
CTCAAACCCA ACGACTTCCG CAACCGCGTC AGTGACTTTG TGAAGGAGAA CGCTCCAGAA 
CACTGGCTGC AGAGCGACTG GCACACCAAA CACATGACCT ACCACAAGAA GTATCCAGAG 
AAGCTGTATT TCGAGGGTCT GGCGGAGCAG GTCAATCCTC CTATCCAGCT GCAGTCTCAG 
TATCTGCCCA TTTACTTCGG CAACGTGTGT CTGCGCTTCC TCCCCGTGTT CGACATCGTC 
ATCCACCGCT TCCTGGAGCT GCTGCCGGTC TCCAAATCCC TGGAGACGCT GCTCGATCAT 
CTGGGAGGAC TCTACAAGTT TCATGATCGG CCGGTGACGT ACCTGTACAA CACGCTGCAT 
TATTATGAGA GGCACCTACG CGACCGCACA AACCTGAAGA GGAAGCTGGT GCATGCCATC 
ATGAGCTCGC TGAAGGACAA TCGAACACCC GGCTGGTGCC TTAGCGAGAC CTACCTGAAG 
TGCGGCATGA ACGCCCGTGA AGATAATGTC TGGATCCCTG ACGACACCTA CTACTGCAAA 
CTCATCGGAC GCCTCGTGGA TACCATGGCA GGTAAATCTC CAGGCCCGTT CCCCAACTGT 
GACTGGAGGT TTAACGAGTT CCCGAACCCG GCCGCTCATG CGCTGCACGT CACCTGTGTG 
GAGCTGATGG CTCTGGCGGT CCCGGGGAAA GACGTGGGCA ATGCTCTGCT TAATGTGGTG 
CTCAAGAGTC AGCCGCTGGT GCCTCGAGAG AACATCACGG CCTGGATGAA CGCCATCGGA 
CTGGTCATCA CCGCTCTGCC TGAGCCCTAC TGGATCGTCC TGCATGACCG TATCGTGAGT 
GTGATCAGCA GTGCGGTGCT CAGCTCTGGG TCAGATTGGC TGGGTTACCC ATTCCAGCTT 
CTGGACTTCA CCGCTTGCCA TCAGTCCTAC TCAGAGATGC ACTGCAGTTA CGTTCTGGCT 
CTGGCTCACG CCGTGTGGCA TCACTCCAGC ATCGGCCAGC TCTCGCTCAT ACCCAAGTTC 
CTGTCGGAGG TCCTGAAGCC CATCGTGAAG ACTGAGTTCC AGCTGCTGTA CGTTTATCAT 
CTGGTGGGCC CGTTTCTGCA GCGCTTCCAG CAGGAGAGAA CCAGATGCAT GCTGGAGATT 
GGAGTGGCGT TTTATGAGAT GCTTCAGGCT GTGGATCAGC ACAGCAACCA CCTGGCCTAC 
ATGGACCCCA TCTGTGACTT CCTGTACCAC ATCAAATACA TGTTTACTGG GGACAGTGTC 
AAAGACCAGG TGGAGCGCAT CATCTGTTCT CTGCGTCCAG CAATGCGTCT TCGTCTACGC 
TTCATCACAC ACATCAGTAA GATGGAGCCG GCGGCGGTGC CCGTGTCCAA CAGCAGCAGC 
GTCCAGCAGA CCAGCAGCGC CTCCTCACCC ACAGCACAGA GCACTGCTGG AGCCGCCATA 
CCACTGTCTG TGACACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mediator MED23 Links Pigmentation and DNA Repair through the Transcription Factor MITF.
Cell reports20(8)1794-1804(2017 Aug)
Xia M,Chen K,Yao X,Xu Y,Yao J,Yan J,Shao Z,Wang G

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Mediator Med23 deficiency enhances neural differentiation of murine embryonic stem cells through modulating BMP signaling.
Development (Cambridge, England)142(3)465-76(2015 Feb)
Zhu W,Yao X,Liang Y,Liang D,Song L,Jing N,Li J,Wang G

Mediator MED23 plays opposing roles in directing smooth muscle cell and adipocyte differentiation.
Genes & development26(19)2192-205(2012 Oct)
Yin JW,Liang Y,Park JY,Chen D,Yao X,Xiao Q,Liu Z,Jiang B,Fu Y,Bao M,Huang Y,Liu Y,Yan J,Zhu MS,Yang Z,Gao P,Tian B,Li D,Wang G

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med23 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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