MED23 cDNA ORF clone, Bos taurus(cattle)

The following MED23 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED23 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa239176 NM_001205762.2
Latest version!
Bos taurus mediator complex subunit 23 (MED23), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237721 XM_024996539.1
Latest version!
Bos taurus mediator complex subunit 23 (MED23), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237722 XM_024996540.1
Latest version!
Bos taurus mediator complex subunit 23 (MED23), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa01658 NM_001205762.1
Latest version!
Bos taurus mediator complex subunit 23 (MED23), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa239176
    Clone ID Related Accession (Same CDS sequence) NM_001205762.2
    Accession Version NM_001205762.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4107bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-27
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 23
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000009.1. On Jun 28, 2018 this sequence version replaced NM_001205762.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5721696.1845.1, SRR5721698.5760.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGACGC AACTACAGAG CATTTTTGAG GAGGTGGTGA AAACGGAGAT TATAGAAGAG 
    GCTTTCCCTG GCATGTTTAT GGACACCCCT GAAGATGAAA AAACAAAACT TATTAGCTGT
    TTAGGGGCCT TCAGACAATT TTGGGCTGGT CTTTCTCAGG AATCTCATGA ACAGTGCATC
    CAGTGGATTG TTAAATTCAT TCATGGCCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT
    GACTGCTTAG CAATGGCAGT CGAGACTGGT CTCCTTCCAC CCAGGATGGT TTGTGAATCC
    CTGATAAACT CTGACACACT TGAATGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA
    CTGGTTCGGA AAATAATTGG AGGGGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG
    ATTTTGGAGA AAATCTTGAC AATTCCCAAT ACAGTGAGCT CAGCTGTTGT ACAGCAGCTT
    TTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCTTGTTT ACTACCAGCA
    TATTTTGCAG TCACAGAGAT CAGGAAACTA TATCCTGAGG GAAAACTTCC ACATTGGTTA
    CTTGGAAACC TGGTATCAGA CTTTGTGGAT ACCTTCAGGC CAACAGCAAG GATAAACTCC
    ATTTGTGGCC GTTGCAGCCT TCTACCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA
    TGGAAATTGG ACCCTACAAC TCTCCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG
    GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTTT TGGAGCAGCC TTATTCCAGG
    GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG
    GAGGACCAGT TGGTGGATCT GGTGGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG
    TTTGATGATG GGGGAACGAG CCAACTCCTC TGGCAGCATC TCTCGAGTCA GCTCATTTTC
    TTTGTGCTTT TCCAGTTTGC AAGTTTCCCA CATATGGTCC TTTCTCTTCA TCAGAAGTTG
    GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCTT GCAATTCATT
    TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTTC CTGTCATGAA GCTCTTCGAC
    CTTCTGTACC CAGAAAAAGA ATTTATCCCA GTTCCTGACA TTAACAAACC CCAGTCAACT
    CATGCCTTTG CCATGACCTG CATTTGGATT CACCTCAACA GAAAAGCCCA AAATGACAAC
    TCCAAACTAC AGATTCCAAT ACCTCACTCC CTAAAGCTTC ACCATGAGTT CCTGCAGCAG
    AGTCTTAGAA ATAAGAGTTT ACAAATGAAT GACTATAAGA TCGCCCTGCT ATGTAATGCA
    TACTCTACAA ATTCAGAATG TTTTACGTTA CCTATGGGAG CTCTGGTGGA GACTATTTAT
    GGAAATGGAA TTATGAGGAT ACCACTTCCT GGAACGAGCT GTTTGGCTTC AGGATCTATT
    ACGCCCTTAC CAATGAACCT CCTGGATTCA CTGACGGTTC ATGCCAAAAT GAGCCTTATC
    CACAGCATTG CTACCAGAGT GATAAAACTT GCTCACGCAA AGTCCAGTGT GGCCTTGGCT
    CCAGCCCTAG TGGAAACTTA CAGCCGTTTA TTGGTCTATA TGGAAATAGA ATCTTTGGGC
    ATCAAAGGAT TTATCAGTCA GCTTCTGCCA ACTGTTTTTA AGTCTCATGC CTGGGGTATC
    TTGCATACGC TTCTTGAGAT GTTCAGCTAC CGGATGCACC ACATTCAGCC CCATTACAGA
    GTTCAGCTCC TAAGTCATCT TCATACTTTG GCTGCAGTTG CACAGACAAA CCAGAACCAG
    CTCCATCTCT GTGTTGAGAG TACTGCACTC AGGCTTATAA CAGCACTAGG TAGCTCAGAA
    GTACAGCCAC AGTTTACACG CTTCCTCAGT GATCCCAAGA CGGTGCTCTC AGCGGAATCC
    GAAGAACTGA ACCGTGCCTT GATACTGACC TTAGCTAGAG CAACTCACGT AACAGATTTT
    TTCACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG
    AGTTTTACAC CTCACAATTG GGCTTCACAT ACTCTTAGTT GTTTTCCAGG CCCACTACAG
    GCATTCTTTA AACAAAATAA TGTACCTCAA GAGAGTCGTT TTAATCTGAA AAAAAATGTG
    GAGGAGGAGT ATAGGAAGTG GAAGTCAATG ACCAACGAAA ATGACATCAT CACCCACTTT
    TCTGCACAAC GTTCTCCTCC TCTCTTCCTT TGTCTGCTCT GGAAAATGCT CCTGGAAACA
    GATCACATTA ATCAGATTGG CTATAGAGTA CTGGAGAGAA TTGGAGCCAG GGCCTTAGTA
    GTCCATGTGA GAACTTTTGC AGATTTCCTG GTATATGAGT TCTCTACGTC AGCTGGAGGT
    CAGCAACTCA ACAAATGCAT TGAAATTCTT AATGATATGG TATGGAAGTA CAACATTGTT
    ACACTGGACA GATTAATTCT CTGCCTGGCC ATGCGTAGCC ACGAAGGGAA TGAAGCCCAA
    GTTTGTTATT TCATAATTCA GTTGCTGTTA CTCAAGCCAA ATGATTTTAG AAATCGAGTA
    AGTGACTTTG TGAAGGAAAA TTCCCCAGAA CACTGGCTAC AGAATGACTG GCACACCAAG
    CACATGAATT ATCACAAGAA ATACCCGGAG AAGCTCTATT TTGAGGGCCT GGCGGAGCAG
    GTTGATCCTC CTGTGCCGAT ACAGTCTCCA TATCTGCCCA TCTATTTTGG AAATGTGTGT
    CTCCGATTCC TCCCAGTGTT TGATATAGTA ATCCACAGGT TTTTAGAGTT GCTCCCGGTA
    TCCAAATCAC TGGAGACTCT ACTAGATCAC CTGGGAGGCT TATATAAATT TCATGATCGT
    CCAGTGACTT ATTTGTATAA TACTCTGCAT TATTATGAAA TGCACCTGAG AAATCGCGAA
    AGTCTCAAAC GAAAACTTGT CCATGCAATC ATTGGGTCGC TCAAGGATAA CCGACCACAG
    GGCTGGTGTC TAAGTGACAC TTACCTTAAA AATGCTATGA ATGCACGAGA AGACAATCCC
    TGGGTCCCAG ACGACTCTTA CTACTGCAAA TTGATTGGCA GACTGGTAGA TACGATGGCT
    GGCAAATCTC CTGGTCCATT CCCAAACTGT GACTGGAGGT TCAATGAGTT TCCCAACCCC
    GCTGCCCATG CACTGCATGT TACTTGTGTG GAACTCATGG CCTTGGCTGT TTCCGGCCAG
    GATGTTGGAA ATGCTCTTCT AAATGTTGTC CTGAAGAGTC AGCCTTTAGT GCCAAGAGAG
    AACATTACAG CATGGATGAA TGCCATTGGA TTGATCATCA CTGCCCTACC AGAGCCGTAT
    TGGATTGTCC TTCACGATCG AATAGTGAGT GTTATCAGCA GTCCCAGCTT GACGTCTGAG
    ACAGAGTGGG TCGGCTATCC TTTCCGGCTC TTCGATTTCA CCGCTTGCCA TCAGTCCTAC
    TCTGAGATGA GCTGTAGCTA CACGTTAGCT CTGGCACATG CAGTGTGGCA CCACTCCAGC
    ATCGGGCAGC TTTCTCTCAT TCCCAAATTT CTCACTGAAG TACTTCTTCC TATAGTGAAG
    ACTGAGTTCC AGTTGCTTTA TGTGTACCAC CTTGTGGGAC CATTTTTACA AAGGTTTCAA
    CAAGAGAGAA CTCGATGTAT GATAGAGATT GGTGTGGCGT TTTATGACAT GTTGCTGAAC
    GTAGACCAGT GTAGCACCCA CTTAAACTAC ATGGATCCCA TCTGTGACTT CCTGTATCAT
    ATGAAGTATA TGTTTACTGG TGACAGTGTA AAAGAACAAG TAGAAAAGAT TATCTGTAAT
    TTAAAACCGG CTTTAAAACT CCGTCTTCGA TTCATCACTC ACATTAGCAA GATGGAACCG
    GCTGCCGTGC CTCCGCAAGC CGTCAGCAGT GGGTCTCCAG CACCTCAGTC TACTCAGGTG
    CCAGGGTCTC TGCCGGTGAC TCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192691.1
    CDS1..4107
    Translation

    Target ORF information:

    RefSeq Version NM_001205762.2
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 23 (MED23), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205762.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGACGC AACTACAGAG CATTTTTGAG GAGGTGGTGA AAACGGAGAT TATAGAAGAG 
    GCTTTCCCTG GCATGTTTAT GGACACCCCT GAAGATGAAA AAACAAAACT TATTAGCTGT
    TTAGGGGCCT TCAGACAATT TTGGGCTGGT CTTTCTCAGG AATCTCATGA ACAGTGCATC
    CAGTGGATTG TTAAATTCAT TCATGGCCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT
    GACTGCTTAG CAATGGCAGT CGAGACTGGT CTCCTTCCAC CCAGGATGGT TTGTGAATCC
    CTGATAAACT CTGACACACT TGAATGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA
    CTGGTTCGGA AAATAATTGG AGGGGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG
    ATTTTGGAGA AAATCTTGAC AATTCCCAAT ACAGTGAGCT CAGCTGTTGT ACAGCAGCTT
    TTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCTTGTTT ACTACCAGCA
    TATTTTGCAG TCACAGAGAT CAGGAAACTA TATCCTGAGG GAAAACTTCC ACATTGGTTA
    CTTGGAAACC TGGTATCAGA CTTTGTGGAT ACCTTCAGGC CAACAGCAAG GATAAACTCC
    ATTTGTGGCC GTTGCAGCCT TCTACCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA
    TGGAAATTGG ACCCTACAAC TCTCCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG
    GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTTT TGGAGCAGCC TTATTCCAGG
    GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG
    GAGGACCAGT TGGTGGATCT GGTGGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG
    TTTGATGATG GGGGAACGAG CCAACTCCTC TGGCAGCATC TCTCGAGTCA GCTCATTTTC
    TTTGTGCTTT TCCAGTTTGC AAGTTTCCCA CATATGGTCC TTTCTCTTCA TCAGAAGTTG
    GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCTT GCAATTCATT
    TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTTC CTGTCATGAA GCTCTTCGAC
    CTTCTGTACC CAGAAAAAGA ATTTATCCCA GTTCCTGACA TTAACAAACC CCAGTCAACT
    CATGCCTTTG CCATGACCTG CATTTGGATT CACCTCAACA GAAAAGCCCA AAATGACAAC
    TCCAAACTAC AGATTCCAAT ACCTCACTCC CTAAAGCTTC ACCATGAGTT CCTGCAGCAG
    AGTCTTAGAA ATAAGAGTTT ACAAATGAAT GACTATAAGA TCGCCCTGCT ATGTAATGCA
    TACTCTACAA ATTCAGAATG TTTTACGTTA CCTATGGGAG CTCTGGTGGA GACTATTTAT
    GGAAATGGAA TTATGAGGAT ACCACTTCCT GGAACGAGCT GTTTGGCTTC AGGATCTATT
    ACGCCCTTAC CAATGAACCT CCTGGATTCA CTGACGGTTC ATGCCAAAAT GAGCCTTATC
    CACAGCATTG CTACCAGAGT GATAAAACTT GCTCACGCAA AGTCCAGTGT GGCCTTGGCT
    CCAGCCCTAG TGGAAACTTA CAGCCGTTTA TTGGTCTATA TGGAAATAGA ATCTTTGGGC
    ATCAAAGGAT TTATCAGTCA GCTTCTGCCA ACTGTTTTTA AGTCTCATGC CTGGGGTATC
    TTGCATACGC TTCTTGAGAT GTTCAGCTAC CGGATGCACC ACATTCAGCC CCATTACAGA
    GTTCAGCTCC TAAGTCATCT TCATACTTTG GCTGCAGTTG CACAGACAAA CCAGAACCAG
    CTCCATCTCT GTGTTGAGAG TACTGCACTC AGGCTTATAA CAGCACTAGG TAGCTCAGAA
    GTACAGCCAC AGTTTACACG CTTCCTCAGT GATCCCAAGA CGGTGCTCTC AGCGGAATCC
    GAAGAACTGA ACCGTGCCTT GATACTGACC TTAGCTAGAG CAACTCACGT AACAGATTTT
    TTCACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG
    AGTTTTACAC CTCACAATTG GGCTTCACAT ACTCTTAGTT GTTTTCCAGG CCCACTACAG
    GCATTCTTTA AACAAAATAA TGTACCTCAA GAGAGTCGTT TTAATCTGAA AAAAAATGTG
    GAGGAGGAGT ATAGGAAGTG GAAGTCAATG ACCAACGAAA ATGACATCAT CACCCACTTT
    TCTGCACAAC GTTCTCCTCC TCTCTTCCTT TGTCTGCTCT GGAAAATGCT CCTGGAAACA
    GATCACATTA ATCAGATTGG CTATAGAGTA CTGGAGAGAA TTGGAGCCAG GGCCTTAGTA
    GTCCATGTGA GAACTTTTGC AGATTTCCTG GTATATGAGT TCTCTACGTC AGCTGGAGGT
    CAGCAACTCA ACAAATGCAT TGAAATTCTT AATGATATGG TATGGAAGTA CAACATTGTT
    ACACTGGACA GATTAATTCT CTGCCTGGCC ATGCGTAGCC ACGAAGGGAA TGAAGCCCAA
    GTTTGTTATT TCATAATTCA GTTGCTGTTA CTCAAGCCAA ATGATTTTAG AAATCGAGTA
    AGTGACTTTG TGAAGGAAAA TTCCCCAGAA CACTGGCTAC AGAATGACTG GCACACCAAG
    CACATGAATT ATCACAAGAA ATACCCGGAG AAGCTCTATT TTGAGGGCCT GGCGGAGCAG
    GTTGATCCTC CTGTGCCGAT ACAGTCTCCA TATCTGCCCA TCTATTTTGG AAATGTGTGT
    CTCCGATTCC TCCCAGTGTT TGATATAGTA ATCCACAGGT TTTTAGAGTT GCTCCCGGTA
    TCCAAATCAC TGGAGACTCT ACTAGATCAC CTGGGAGGCT TATATAAATT TCATGATCGT
    CCAGTGACTT ATTTGTATAA TACTCTGCAT TATTATGAAA TGCACCTGAG AAATCGCGAA
    AGTCTCAAAC GAAAACTTGT CCATGCAATC ATTGGGTCGC TCAAGGATAA CCGACCACAG
    GGCTGGTGTC TAAGTGACAC TTACCTTAAA AATGCTATGA ATGCACGAGA AGACAATCCC
    TGGGTCCCAG ACGACTCTTA CTACTGCAAA TTGATTGGCA GACTGGTAGA TACGATGGCT
    GGCAAATCTC CTGGTCCATT CCCAAACTGT GACTGGAGGT TCAATGAGTT TCCCAACCCC
    GCTGCCCATG CACTGCATGT TACTTGTGTG GAACTCATGG CCTTGGCTGT TTCCGGCCAG
    GATGTTGGAA ATGCTCTTCT AAATGTTGTC CTGAAGAGTC AGCCTTTAGT GCCAAGAGAG
    AACATTACAG CATGGATGAA TGCCATTGGA TTGATCATCA CTGCCCTACC AGAGCCGTAT
    TGGATTGTCC TTCACGATCG AATAGTGAGT GTTATCAGCA GTCCCAGCTT GACGTCTGAG
    ACAGAGTGGG TCGGCTATCC TTTCCGGCTC TTCGATTTCA CCGCTTGCCA TCAGTCCTAC
    TCTGAGATGA GCTGTAGCTA CACGTTAGCT CTGGCACATG CAGTGTGGCA CCACTCCAGC
    ATCGGGCAGC TTTCTCTCAT TCCCAAATTT CTCACTGAAG TACTTCTTCC TATAGTGAAG
    ACTGAGTTCC AGTTGCTTTA TGTGTACCAC CTTGTGGGAC CATTTTTACA AAGGTTTCAA
    CAAGAGAGAA CTCGATGTAT GATAGAGATT GGTGTGGCGT TTTATGACAT GTTGCTGAAC
    GTAGACCAGT GTAGCACCCA CTTAAACTAC ATGGATCCCA TCTGTGACTT CCTGTATCAT
    ATGAAGTATA TGTTTACTGG TGACAGTGTA AAAGAACAAG TAGAAAAGAT TATCTGTAAT
    TTAAAACCGG CTTTAAAACT CCGTCTTCGA TTCATCACTC ACATTAGCAA GATGGAACCG
    GCTGCCGTGC CTCCGCAAGC CGTCAGCAGT GGGTCTCCAG CACCTCAGTC TACTCAGGTG
    CCAGGGTCTC TGCCGGTGAC TCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237721
    Clone ID Related Accession (Same CDS sequence) XM_024996539.1
    Accession Version XM_024996539.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4134bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 23 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTCCAGA TGGAGACGCA ACTACAGAGC ATTTTTGAGG AGGTGGTGAA AACGGAGATT 
    ATAGAAGAGG CTTTCCCTGG CATGTTTATG GACACCCCTG AAGATGAAAA AACAAAACTT
    ATTAGCTGTT TAGGGGCCTT CAGACAATTT TGGGCTGGTC TTTCTCAGGA ATCTCATGAA
    CAGTGCATCC AGTGGATTGT TAAATTCATT CATGGCCAGC ATAGTCCTAA AAGAATTTCT
    TTTCTTTATG ACTGCTTAGC AATGGCAGTC GAGACTGGTC TCCTTCCACC CAGGATGGTT
    TGTGAATCCC TGATAAACTC TGACACACTT GAATGGGAAA GAACACAGCT TTGGGCCTTA
    ACATTTAAAC TGGTTCGGAA AATAATTGGA GGGGTGGATT ACAAGGGTGT TCGAGATCTC
    TTAAAAGTGA TTTTGGAGAA AATCTTGACA ATTCCCAATA CAGTGAGCTC AGCTGTTGTA
    CAGCAGCTTT TGGCAGCAAG AGAGGTTATA GCATATATCT TGGAAAGAAA TGCTTGTTTA
    CTACCAGCAT ATTTTGCAGT CACAGAGATC AGGAAACTAT ATCCTGAGGG AAAACTTCCA
    CATTGGTTAC TTGGAAACCT GGTATCAGAC TTTGTGGATA CCTTCAGGCC AACAGCAAGG
    ATAAACTCCA TTTGTGGCCG TTGCAGCCTT CTACCAGTTG TAAATAATTC GGGTGCCATT
    TGTAATTCAT GGAAATTGGA CCCTACAACT CTCCGTTTTC CTTTGAAAGG CCTTTTGCCA
    TATGATAAGG ATCTGTTTGA ACCACAGACT GCTTTGTTGA GATATGTTTT GGAGCAGCCT
    TATTCCAGGG ATATGGTCTG CAATATGCTA GGTTTAAATA AGCAGACCTT GAACATTGCT
    CAGCACAAGC AGCGCTGCCC TGTGCTGGAG GACCAGTTGG TGGATCTGGT GGTTTATGCC
    ATGGAGCGAT CTGAGACCGA GGAGAAGTTT GATGATGGGG GAACGAGCCA ACTCCTCTGG
    CAGCATCTCT CGAGTCAGCT CATTTTCTTT GTGCTTTTCC AGTTTGCAAG TTTCCCACAT
    ATGGTCCTTT CTCTTCATCA GAAGTTGGCA GGGCGAGGAC TGATTAAAGG CAGAGATCAT
    CTTATGTGGG TTCTCTTGCA ATTCATTTCT GGAAGTATTC AGAAAAATGC ACTAGCTGAT
    TTTCTTCCTG TCATGAAGCT CTTCGACCTT CTGTACCCAG AAAAAGAATT TATCCCAGTT
    CCTGACATTA ACAAACCCCA GTCAACTCAT GCCTTTGCCA TGACCTGCAT TTGGATTCAC
    CTCAACAGAA AAGCCCAAAA TGACAACTCC AAACTACAGA TTCCAATACC TCACTCCCTA
    AAGCTTCACC ATGAGTTCCT GCAGCAGAGT CTTAGAAATA AGAGTTTACA AATGAATGAC
    TATAAGATCG CCCTGCTATG TAATGCATAC TCTACAAATT CAGAATGTTT TACGTTACCT
    ATGGGAGCTC TGGTGGAGAC TATTTATGGA AATGGAATTA TGAGGATACC ACTTCCTGGA
    ACGAGCTGTT TGGCTTCAGG ATCTATTACG CCCTTACCAA TGAACCTCCT GGATTCACTG
    ACGGTTCATG CCAAAATGAG CCTTATCCAC AGCATTGCTA CCAGAGTGAT AAAACTTGCT
    CACGCAAAGT CCAGTGTGGC CTTGGCTCCA GCCCTAGTGG AAACTTACAG CCGTTTATTG
    GTCTATATGG AAATAGAATC TTTGGGCATC AAAGGATTTA TCAGTCAGCT TCTGCCAACT
    GTTTTTAAGT CTCATGCCTG GGGTATCTTG CATACGCTTC TTGAGATGTT CAGCTACCGG
    ATGCACCACA TTCAGCCCCA TTACAGAGTT CAGCTCCTAA GTCATCTTCA TACTTTGGCT
    GCAGTTGCAC AGACAAACCA GAACCAGCTC CATCTCTGTG TTGAGAGTAC TGCACTCAGG
    CTTATAACAG CACTAGGTAG CTCAGAAGTA CAGCCACAGT TTACACGCTT CCTCAGTGAT
    CCCAAGACGG TGCTCTCAGC GGAATCCGAA GAACTGAACC GTGCCTTGAT ACTGACCTTA
    GCTAGAGCAA CTCACGTAAC AGATTTTTTC ACAGGCTCTG ATTCAATTCA GGGAACTTGG
    TGTAAAGACA TACTTCAGAC CATCATGAGT TTTACACCTC ACAATTGGGC TTCACATACT
    CTTAGTTGTT TTCCAGGCCC ACTACAGGCA TTCTTTAAAC AAAATAATGT ACCTCAAGAG
    AGTCGTTTTA ATCTGAAAAA AAATGTGGAG GAGGAGTATA GGAAGTGGAA GTCAATGACC
    AACGAAAATG ACATCATCAC CCACTTTTCT GCACAACGTT CTCCTCCTCT CTTCCTTTGT
    CTGCTCTGGA AAATGCTCCT GGAAACAGAT CACATTAATC AGATTGGCTA TAGAGTACTG
    GAGAGAATTG GAGCCAGGGC CTTAGTAGTC CATGTGAGAA CTTTTGCAGA TTTCCTGGTA
    TATGAGTTCT CTACGTCAGC TGGAGGTCAG CAACTCAACA AATGCATTGA AATTCTTAAT
    GATATGGTAT GGAAGTACAA CATTGTTACA CTGGACAGAT TAATTCTCTG CCTGGCCATG
    CGTAGCCACG AAGGGAATGA AGCCCAAGTT TGTTATTTCA TAATTCAGTT GCTGTTACTC
    AAGCCAAATG ATTTTAGAAA TCGAGTAAGT GACTTTGTGA AGGAAAATTC CCCAGAACAC
    TGGCTACAGA ATGACTGGCA CACCAAGCAC ATGAATTATC ACAAGAAATA CCCGGAGAAG
    CTCTATTTTG AGGGCCTGGC GGAGCAGGTT GATCCTCCTG TGCCGATACA GTCTCCATAT
    CTGCCCATCT ATTTTGGAAA TGTGTGTCTC CGATTCCTCC CAGTGTTTGA TATAGTAATC
    CACAGGTTTT TAGAGTTGCT CCCGGTATCC AAATCACTGG AGACTCTACT AGATCACCTG
    GGAGGCTTAT ATAAATTTCA TGATCGTCCA GTGACTTATT TGTATAATAC TCTGCATTAT
    TATGAAATGC ACCTGAGAAA TCGCGAAAGT CTCAAACGAA AACTTGTCCA TGCAATCATT
    GGGTCGCTCA AGGATAACCG ACCACAGGGC TGGTGTCTAA GTGACACTTA CCTTAAAAAT
    GCTATGAATG CACGAGAAGA CAATCCCTGG GTCCCAGACG ACTCTTACTA CTGCAAATTG
    ATTGGCAGAC TGGTAGATAC GATGGCTGGC AAATCTCCTG GTCCATTCCC AAACTGTGAC
    TGGAGGTTCA ATGAGTTTCC CAACCCCGCT GCCCATGCAC TGCATGTTAC TTGTGTGGAA
    CTCATGGCCT TGGCTGTTTC CGGCCAGGAT GTTGGAAATG CTCTTCTAAA TGTTGTCCTG
    AAGAGTCAGC CTTTAGTGCC AAGAGAGAAC ATTACAGCAT GGATGAATGC CATTGGATTG
    ATCATCACTG CCCTACCAGA GCCGTATTGG ATTGTCCTTC ACGATCGAAT AGTGAGTGTT
    ATCAGCAGTC CCAGCTTGAC GTCTGAGACA GAGTGGGTCG GCTATCCTTT CCGGCTCTTC
    GATTTCACCG CTTGCCATCA GTCCTACTCT GAGATGAGCT GTAGCTACAC GTTAGCTCTG
    GCACATGCAG TGTGGCACCA CTCCAGCATC GGGCAGCTTT CTCTCATTCC CAAATTTCTC
    ACTGAAGTAC TTCTTCCTAT AGTGAAGACT GAGTTCCAGT TGCTTTATGT GTACCACCTT
    GTGGGACCAT TTTTACAAAG GTTTCAACAA GAGAGAACTC GATGTATGAT AGAGATTGGT
    GTGGCGTTTT ATGACATGTT GCTGAACGTA GACCAGTGTA GCACCCACTT AAACTACATG
    GATCCCATCT GTGACTTCCT GTATCATATG AAGTATATGT TTACTGGTGA CAGTGTAAAA
    GAACAAGTAG AAAAGATTAT CTGTAATTTA AAACCGGCTT TAAAACTCCG TCTTCGATTC
    ATCACTCACA TTAGCAAGAT GGAACCGGCT GCCGTGCCTC CGCAAGCCGT CAGCAGTGGG
    TCTCCAGCAC CTCAGTCTAC TCAGGTGCCA GGGTCTCTGC CGGTGACTCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852307.1
    CDS216..4349
    Translation

    Target ORF information:

    RefSeq Version XM_024996539.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 23 (MED23), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996539.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTCCAGA TGGAGACGCA ACTACAGAGC ATTTTTGAGG AGGTGGTGAA AACGGAGATT 
    ATAGAAGAGG CTTTCCCTGG CATGTTTATG GACACCCCTG AAGATGAAAA AACAAAACTT
    ATTAGCTGTT TAGGGGCCTT CAGACAATTT TGGGCTGGTC TTTCTCAGGA ATCTCATGAA
    CAGTGCATCC AGTGGATTGT TAAATTCATT CATGGCCAGC ATAGTCCTAA AAGAATTTCT
    TTTCTTTATG ACTGCTTAGC AATGGCAGTC GAGACTGGTC TCCTTCCACC CAGGATGGTT
    TGTGAATCCC TGATAAACTC TGACACACTT GAATGGGAAA GAACACAGCT TTGGGCCTTA
    ACATTTAAAC TGGTTCGGAA AATAATTGGA GGGGTGGATT ACAAGGGTGT TCGAGATCTC
    TTAAAAGTGA TTTTGGAGAA AATCTTGACA ATTCCCAATA CAGTGAGCTC AGCTGTTGTA
    CAGCAGCTTT TGGCAGCAAG AGAGGTTATA GCATATATCT TGGAAAGAAA TGCTTGTTTA
    CTACCAGCAT ATTTTGCAGT CACAGAGATC AGGAAACTAT ATCCTGAGGG AAAACTTCCA
    CATTGGTTAC TTGGAAACCT GGTATCAGAC TTTGTGGATA CCTTCAGGCC AACAGCAAGG
    ATAAACTCCA TTTGTGGCCG TTGCAGCCTT CTACCAGTTG TAAATAATTC GGGTGCCATT
    TGTAATTCAT GGAAATTGGA CCCTACAACT CTCCGTTTTC CTTTGAAAGG CCTTTTGCCA
    TATGATAAGG ATCTGTTTGA ACCACAGACT GCTTTGTTGA GATATGTTTT GGAGCAGCCT
    TATTCCAGGG ATATGGTCTG CAATATGCTA GGTTTAAATA AGCAGACCTT GAACATTGCT
    CAGCACAAGC AGCGCTGCCC TGTGCTGGAG GACCAGTTGG TGGATCTGGT GGTTTATGCC
    ATGGAGCGAT CTGAGACCGA GGAGAAGTTT GATGATGGGG GAACGAGCCA ACTCCTCTGG
    CAGCATCTCT CGAGTCAGCT CATTTTCTTT GTGCTTTTCC AGTTTGCAAG TTTCCCACAT
    ATGGTCCTTT CTCTTCATCA GAAGTTGGCA GGGCGAGGAC TGATTAAAGG CAGAGATCAT
    CTTATGTGGG TTCTCTTGCA ATTCATTTCT GGAAGTATTC AGAAAAATGC ACTAGCTGAT
    TTTCTTCCTG TCATGAAGCT CTTCGACCTT CTGTACCCAG AAAAAGAATT TATCCCAGTT
    CCTGACATTA ACAAACCCCA GTCAACTCAT GCCTTTGCCA TGACCTGCAT TTGGATTCAC
    CTCAACAGAA AAGCCCAAAA TGACAACTCC AAACTACAGA TTCCAATACC TCACTCCCTA
    AAGCTTCACC ATGAGTTCCT GCAGCAGAGT CTTAGAAATA AGAGTTTACA AATGAATGAC
    TATAAGATCG CCCTGCTATG TAATGCATAC TCTACAAATT CAGAATGTTT TACGTTACCT
    ATGGGAGCTC TGGTGGAGAC TATTTATGGA AATGGAATTA TGAGGATACC ACTTCCTGGA
    ACGAGCTGTT TGGCTTCAGG ATCTATTACG CCCTTACCAA TGAACCTCCT GGATTCACTG
    ACGGTTCATG CCAAAATGAG CCTTATCCAC AGCATTGCTA CCAGAGTGAT AAAACTTGCT
    CACGCAAAGT CCAGTGTGGC CTTGGCTCCA GCCCTAGTGG AAACTTACAG CCGTTTATTG
    GTCTATATGG AAATAGAATC TTTGGGCATC AAAGGATTTA TCAGTCAGCT TCTGCCAACT
    GTTTTTAAGT CTCATGCCTG GGGTATCTTG CATACGCTTC TTGAGATGTT CAGCTACCGG
    ATGCACCACA TTCAGCCCCA TTACAGAGTT CAGCTCCTAA GTCATCTTCA TACTTTGGCT
    GCAGTTGCAC AGACAAACCA GAACCAGCTC CATCTCTGTG TTGAGAGTAC TGCACTCAGG
    CTTATAACAG CACTAGGTAG CTCAGAAGTA CAGCCACAGT TTACACGCTT CCTCAGTGAT
    CCCAAGACGG TGCTCTCAGC GGAATCCGAA GAACTGAACC GTGCCTTGAT ACTGACCTTA
    GCTAGAGCAA CTCACGTAAC AGATTTTTTC ACAGGCTCTG ATTCAATTCA GGGAACTTGG
    TGTAAAGACA TACTTCAGAC CATCATGAGT TTTACACCTC ACAATTGGGC TTCACATACT
    CTTAGTTGTT TTCCAGGCCC ACTACAGGCA TTCTTTAAAC AAAATAATGT ACCTCAAGAG
    AGTCGTTTTA ATCTGAAAAA AAATGTGGAG GAGGAGTATA GGAAGTGGAA GTCAATGACC
    AACGAAAATG ACATCATCAC CCACTTTTCT GCACAACGTT CTCCTCCTCT CTTCCTTTGT
    CTGCTCTGGA AAATGCTCCT GGAAACAGAT CACATTAATC AGATTGGCTA TAGAGTACTG
    GAGAGAATTG GAGCCAGGGC CTTAGTAGTC CATGTGAGAA CTTTTGCAGA TTTCCTGGTA
    TATGAGTTCT CTACGTCAGC TGGAGGTCAG CAACTCAACA AATGCATTGA AATTCTTAAT
    GATATGGTAT GGAAGTACAA CATTGTTACA CTGGACAGAT TAATTCTCTG CCTGGCCATG
    CGTAGCCACG AAGGGAATGA AGCCCAAGTT TGTTATTTCA TAATTCAGTT GCTGTTACTC
    AAGCCAAATG ATTTTAGAAA TCGAGTAAGT GACTTTGTGA AGGAAAATTC CCCAGAACAC
    TGGCTACAGA ATGACTGGCA CACCAAGCAC ATGAATTATC ACAAGAAATA CCCGGAGAAG
    CTCTATTTTG AGGGCCTGGC GGAGCAGGTT GATCCTCCTG TGCCGATACA GTCTCCATAT
    CTGCCCATCT ATTTTGGAAA TGTGTGTCTC CGATTCCTCC CAGTGTTTGA TATAGTAATC
    CACAGGTTTT TAGAGTTGCT CCCGGTATCC AAATCACTGG AGACTCTACT AGATCACCTG
    GGAGGCTTAT ATAAATTTCA TGATCGTCCA GTGACTTATT TGTATAATAC TCTGCATTAT
    TATGAAATGC ACCTGAGAAA TCGCGAAAGT CTCAAACGAA AACTTGTCCA TGCAATCATT
    GGGTCGCTCA AGGATAACCG ACCACAGGGC TGGTGTCTAA GTGACACTTA CCTTAAAAAT
    GCTATGAATG CACGAGAAGA CAATCCCTGG GTCCCAGACG ACTCTTACTA CTGCAAATTG
    ATTGGCAGAC TGGTAGATAC GATGGCTGGC AAATCTCCTG GTCCATTCCC AAACTGTGAC
    TGGAGGTTCA ATGAGTTTCC CAACCCCGCT GCCCATGCAC TGCATGTTAC TTGTGTGGAA
    CTCATGGCCT TGGCTGTTTC CGGCCAGGAT GTTGGAAATG CTCTTCTAAA TGTTGTCCTG
    AAGAGTCAGC CTTTAGTGCC AAGAGAGAAC ATTACAGCAT GGATGAATGC CATTGGATTG
    ATCATCACTG CCCTACCAGA GCCGTATTGG ATTGTCCTTC ACGATCGAAT AGTGAGTGTT
    ATCAGCAGTC CCAGCTTGAC GTCTGAGACA GAGTGGGTCG GCTATCCTTT CCGGCTCTTC
    GATTTCACCG CTTGCCATCA GTCCTACTCT GAGATGAGCT GTAGCTACAC GTTAGCTCTG
    GCACATGCAG TGTGGCACCA CTCCAGCATC GGGCAGCTTT CTCTCATTCC CAAATTTCTC
    ACTGAAGTAC TTCTTCCTAT AGTGAAGACT GAGTTCCAGT TGCTTTATGT GTACCACCTT
    GTGGGACCAT TTTTACAAAG GTTTCAACAA GAGAGAACTC GATGTATGAT AGAGATTGGT
    GTGGCGTTTT ATGACATGTT GCTGAACGTA GACCAGTGTA GCACCCACTT AAACTACATG
    GATCCCATCT GTGACTTCCT GTATCATATG AAGTATATGT TTACTGGTGA CAGTGTAAAA
    GAACAAGTAG AAAAGATTAT CTGTAATTTA AAACCGGCTT TAAAACTCCG TCTTCGATTC
    ATCACTCACA TTAGCAAGAT GGAACCGGCT GCCGTGCCTC CGCAAGCCGT CAGCAGTGGG
    TCTCCAGCAC CTCAGTCTAC TCAGGTGCCA GGGTCTCTGC CGGTGACTCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237722
    Clone ID Related Accession (Same CDS sequence) XM_024996540.1
    Accession Version XM_024996540.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3516bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 23 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCCAGA TGGAGACGCA ACTACAGAGC ATTTTTGAGG AGGTGGTGAA AACGGAGATT 
    ATAGAAGAGG CTTTCCCTGG CATGTTTATG GACACCCCTG AAGATGAAAA AACAAAACTT
    ATTAGCTGTT TAGGGGCCTT CAGACAATTT TGGGCTGGTC TTTCTCAGGA ATCTCATGAA
    CAGTGCATCC AGTGGATTGT TAAATTCATT CATGGCCAGC ATAGTCCTAA AAGAATTTCT
    TTTCTTTATG ACTGCTTAGC AATGGCAGTC GAGACTGGTC TCCTTCCACC CAGGATGGTT
    TGTGAATCCC TGATAAACTC TGACACACTT GAATGGGAAA GAACACAGCT TTGGGCCTTA
    ACATTTAAAC TGGTTCGGAA AATAATTGGA GGGGTGGATT ACAAGGGTGT TCGAGATCTC
    TTAAAAGTGA TTTTGGAGAA AATCTTGACA ATTCCCAATA CAGTGAGCTC AGCTGTTGTA
    CAGCAGCTTT TGGCAGCAAG AGAGGTTATA GCATATATCT TGGAAAGAAA TGCTTGTTTA
    CTACCAGCAT ATTTTGCAGT CACAGAGATC AGGAAACTAT ATCCTGAGGG AAAACTTCCA
    CATTGGTTAC TTGGAAACCT GGTATCAGAC TTTGTGGATA CCTTCAGGCC AACAGCAAGG
    ATAAACTCCA TTTGTGGCCG TTGCAGCCTT CTACCAGTTG TAAATAATTC GGGTGCCATT
    TGTAATTCAT GGAAATTGGA CCCTACAACT CTCCGTTTTC CTTTGAAAGG CCTTTTGCCA
    TATGATAAGG ATCTGTTTGA ACCACAGACT GCTTTGTTGA GATATGTTTT GGAGCAGCCT
    TATTCCAGGG ATATGGTCTG CAATATGCTA GGTTTAAATA AGCAGACCTT GAACATTGCT
    CAGCACAAGC AGCGCTGCCC TGTGCTGGAG GACCAGTTGG TGGATCTGGT GGTTTATGCC
    ATGGAGCGAT CTGAGACCGA GGAGAAGTTT GATGATGGGG GAACGAGCCA ACTCCTCTGG
    CAGCATCTCT CGAGTCAGCT CATTTTCTTT GTGCTTTTCC AGTTTGCAAG TTTCCCACAT
    ATGGTCCTTT CTCTTCATCA GAAGTTGGCA GGGCGAGGAC TGATTAAAGG CAGAGATCAT
    CTTATGTGGG TTCTCTTGCA ATTCATTTCT GGAAGTATTC AGAAAAATGC ACTAGCTGAT
    TTTCTTCCTG TCATGAAGCT CTTCGACCTT CTGTACCCAG AAAAAGAATT TATCCCAGTT
    CCTGACATTA ACAAACCCCA GTCAACTCAT GCCTTTGCCA TGACCTGCAT TTGGATTCAC
    CTCAACAGAA AAGCCCAAAA TGACAACTCC AAACTACAGA TTCCAATACC TCACTCCCTA
    AAGCTTCACC ATGAGTTCCT GCAGCAGAGT CTTAGAAATA AGAGTTTACA AATGAATGAC
    TATAAGATCG CCCTGCTATG TAATGCATAC TCTACAAATT CAGAATGTTT TACGTTACCT
    ATGGGAGCTC TGGTGGAGAC TATTTATGGA AATGGAATTA TGAGGATACC ACTTCCTGGA
    ACGAGCTGTT TGGCTTCAGG ATCTATTACG CCCTTACCAA TGAACCTCCT GGATTCACTG
    ACGGTTCATG CCAAAATGAG CCTTATCCAC AGCATTGCTA CCAGAGTGAT AAAACTTGCT
    CACGCAAAGT CCAGTGTGGC CTTGGCTCCA GCCCTAGTGG AAACTTACAG CCGTTTATTG
    GTCTATATGG AAATAGAATC TTTGGGCATC AAAGGATTTA TCAGTCAGCT TCTGCCAACT
    GTTTTTAAGT CTCATGCCTG GGGTATCTTG CATACGCTTC TTGAGATGTT CAGCTACCGG
    ATGCACCACA TTCAGCCCCA TTACAGAGTT CAGCTCCTAA GTCATCTTCA TACTTTGGCT
    GCAGTTGCAC AGACAAACCA GAACCAGCTC CATCTCTGTG TTGAGAGTAC TGCACTCAGG
    CTTATAACAG CACTAGGTAG CTCAGAAGTA CAGCCACAGT TTACACGCTT CCTCAGTGAT
    CCCAAGACGG TGCTCTCAGC GGAATCCGAA GAACTGAACC GTGCCTTGAT ACTGACCTTA
    GCTAGAGCAA CTCACGTAAC AGATTTTTTC ACAGGCTCTG ATTCAATTCA GGGAACTTGG
    TGTAAAGACA TACTTCAGAC CATCATGAGT TTTACACCTC ACAATTGGGC TTCACATACT
    CTTAGTTGTT TTCCAGGCCC ACTACAGGCA TTCTTTAAAC AAAATAATGT ACCTCAAGAG
    AGTCGTTTTA ATCTGAAAAA AAATGTGGAG GAGGAGTATA GGAAGTGGAA GTCAATGACC
    AACGAAAATG ACATCATCAC CCACTTTTCT GCACAACGTT CTCCTCCTCT CTTCCTTTGT
    CTGCTCTGGA AAATGCTCCT GGAAACAGAT CACATTAATC AGATTGGCTA TAGAGTACTG
    GAGAGAATTG GAGCCAGGGC CTTAGTAGTC CATGTGAGAA CTTTTGCAGA TTTCCTGGTA
    TATGAGTTCT CTACGTCAGC TGGAGGTCAG CAACTCAACA AATGCATTGA AATTCTTAAT
    GATATGGTAT GGAAGTACAA CATTGTTACA CTGGACAGAT TAATTCTCTG CCTGGCCATG
    CGTAGCCACG AAGGGAATGA AGCCCAAGTT TGTTATTTCA TAATTCAGTT GCTGTTACTC
    AAGCCAAATG ATTTTAGAAA TCGAGTAAGT GACTTTGTGA AGGAAAATTC CCCAGAACAC
    TGGCTACAGA ATGACTGGCA CACCAAGCAC ATGAATTATC ACAAGAAATA CCCGGAGAAG
    CTCTATTTTG AGGGCCTGGC GGAGCAGGTT GATCCTCCTG TGCCGATACA GTCTCCATAT
    CTGCCCATCT ATTTTGGAAA TGTGTGTCTC CGATTCCTCC CAGTGTTTGA TATAGTAATC
    CACAGGTTTT TAGAGTTGCT CCCGGTATCC AAATCACTGG AGACTCTACT AGATCACCTG
    GGAGGCTTAT ATAAATTTCA TGATCGTCCA GTGACTTATT TGTATAATAC TCTGCATTAT
    TATGAAATGC ACCTGAGAAA TCGCGAAAGT CTCAAACGAA AACTTGTCCA TGCAATCATT
    GGGTCGCTCA AGGATAACCG ACCACAGGGC TGGTGTCTAA GTGACACTTA CCTTAAAAAT
    GCTATGAATG CACGAGAAGA CAATCCCTGG GTCCCAGACG ACTCTTACTA CTGCAAATTG
    ATTGGCAGAC TGGTAGATAC GATGGCTGGC AAATCTCCTG GTCCATTCCC AAACTGTGAC
    TGGAGGTTCA ATGAGTTTCC CAACCCCGCT GCCCATGCAC TGCATGTTAC TTGTGTGGAA
    CTCATGGCCT TGGCTGTTTC CGGCCAGGAT GTTGGAAATG CTCTTCTAAA TGTTGTCCTG
    AAGAGTCAGC CTTTAGTGCC AAGAGAGAAC ATTACAGCAT GGATGAATGC CATTGGATTG
    ATCATCACTG CCCTACCAGC TTTTTCTTGG TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852308.1
    CDS218..3733
    Translation

    Target ORF information:

    RefSeq Version XM_024996540.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 23 (MED23), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996540.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCCAGA TGGAGACGCA ACTACAGAGC ATTTTTGAGG AGGTGGTGAA AACGGAGATT 
    ATAGAAGAGG CTTTCCCTGG CATGTTTATG GACACCCCTG AAGATGAAAA AACAAAACTT
    ATTAGCTGTT TAGGGGCCTT CAGACAATTT TGGGCTGGTC TTTCTCAGGA ATCTCATGAA
    CAGTGCATCC AGTGGATTGT TAAATTCATT CATGGCCAGC ATAGTCCTAA AAGAATTTCT
    TTTCTTTATG ACTGCTTAGC AATGGCAGTC GAGACTGGTC TCCTTCCACC CAGGATGGTT
    TGTGAATCCC TGATAAACTC TGACACACTT GAATGGGAAA GAACACAGCT TTGGGCCTTA
    ACATTTAAAC TGGTTCGGAA AATAATTGGA GGGGTGGATT ACAAGGGTGT TCGAGATCTC
    TTAAAAGTGA TTTTGGAGAA AATCTTGACA ATTCCCAATA CAGTGAGCTC AGCTGTTGTA
    CAGCAGCTTT TGGCAGCAAG AGAGGTTATA GCATATATCT TGGAAAGAAA TGCTTGTTTA
    CTACCAGCAT ATTTTGCAGT CACAGAGATC AGGAAACTAT ATCCTGAGGG AAAACTTCCA
    CATTGGTTAC TTGGAAACCT GGTATCAGAC TTTGTGGATA CCTTCAGGCC AACAGCAAGG
    ATAAACTCCA TTTGTGGCCG TTGCAGCCTT CTACCAGTTG TAAATAATTC GGGTGCCATT
    TGTAATTCAT GGAAATTGGA CCCTACAACT CTCCGTTTTC CTTTGAAAGG CCTTTTGCCA
    TATGATAAGG ATCTGTTTGA ACCACAGACT GCTTTGTTGA GATATGTTTT GGAGCAGCCT
    TATTCCAGGG ATATGGTCTG CAATATGCTA GGTTTAAATA AGCAGACCTT GAACATTGCT
    CAGCACAAGC AGCGCTGCCC TGTGCTGGAG GACCAGTTGG TGGATCTGGT GGTTTATGCC
    ATGGAGCGAT CTGAGACCGA GGAGAAGTTT GATGATGGGG GAACGAGCCA ACTCCTCTGG
    CAGCATCTCT CGAGTCAGCT CATTTTCTTT GTGCTTTTCC AGTTTGCAAG TTTCCCACAT
    ATGGTCCTTT CTCTTCATCA GAAGTTGGCA GGGCGAGGAC TGATTAAAGG CAGAGATCAT
    CTTATGTGGG TTCTCTTGCA ATTCATTTCT GGAAGTATTC AGAAAAATGC ACTAGCTGAT
    TTTCTTCCTG TCATGAAGCT CTTCGACCTT CTGTACCCAG AAAAAGAATT TATCCCAGTT
    CCTGACATTA ACAAACCCCA GTCAACTCAT GCCTTTGCCA TGACCTGCAT TTGGATTCAC
    CTCAACAGAA AAGCCCAAAA TGACAACTCC AAACTACAGA TTCCAATACC TCACTCCCTA
    AAGCTTCACC ATGAGTTCCT GCAGCAGAGT CTTAGAAATA AGAGTTTACA AATGAATGAC
    TATAAGATCG CCCTGCTATG TAATGCATAC TCTACAAATT CAGAATGTTT TACGTTACCT
    ATGGGAGCTC TGGTGGAGAC TATTTATGGA AATGGAATTA TGAGGATACC ACTTCCTGGA
    ACGAGCTGTT TGGCTTCAGG ATCTATTACG CCCTTACCAA TGAACCTCCT GGATTCACTG
    ACGGTTCATG CCAAAATGAG CCTTATCCAC AGCATTGCTA CCAGAGTGAT AAAACTTGCT
    CACGCAAAGT CCAGTGTGGC CTTGGCTCCA GCCCTAGTGG AAACTTACAG CCGTTTATTG
    GTCTATATGG AAATAGAATC TTTGGGCATC AAAGGATTTA TCAGTCAGCT TCTGCCAACT
    GTTTTTAAGT CTCATGCCTG GGGTATCTTG CATACGCTTC TTGAGATGTT CAGCTACCGG
    ATGCACCACA TTCAGCCCCA TTACAGAGTT CAGCTCCTAA GTCATCTTCA TACTTTGGCT
    GCAGTTGCAC AGACAAACCA GAACCAGCTC CATCTCTGTG TTGAGAGTAC TGCACTCAGG
    CTTATAACAG CACTAGGTAG CTCAGAAGTA CAGCCACAGT TTACACGCTT CCTCAGTGAT
    CCCAAGACGG TGCTCTCAGC GGAATCCGAA GAACTGAACC GTGCCTTGAT ACTGACCTTA
    GCTAGAGCAA CTCACGTAAC AGATTTTTTC ACAGGCTCTG ATTCAATTCA GGGAACTTGG
    TGTAAAGACA TACTTCAGAC CATCATGAGT TTTACACCTC ACAATTGGGC TTCACATACT
    CTTAGTTGTT TTCCAGGCCC ACTACAGGCA TTCTTTAAAC AAAATAATGT ACCTCAAGAG
    AGTCGTTTTA ATCTGAAAAA AAATGTGGAG GAGGAGTATA GGAAGTGGAA GTCAATGACC
    AACGAAAATG ACATCATCAC CCACTTTTCT GCACAACGTT CTCCTCCTCT CTTCCTTTGT
    CTGCTCTGGA AAATGCTCCT GGAAACAGAT CACATTAATC AGATTGGCTA TAGAGTACTG
    GAGAGAATTG GAGCCAGGGC CTTAGTAGTC CATGTGAGAA CTTTTGCAGA TTTCCTGGTA
    TATGAGTTCT CTACGTCAGC TGGAGGTCAG CAACTCAACA AATGCATTGA AATTCTTAAT
    GATATGGTAT GGAAGTACAA CATTGTTACA CTGGACAGAT TAATTCTCTG CCTGGCCATG
    CGTAGCCACG AAGGGAATGA AGCCCAAGTT TGTTATTTCA TAATTCAGTT GCTGTTACTC
    AAGCCAAATG ATTTTAGAAA TCGAGTAAGT GACTTTGTGA AGGAAAATTC CCCAGAACAC
    TGGCTACAGA ATGACTGGCA CACCAAGCAC ATGAATTATC ACAAGAAATA CCCGGAGAAG
    CTCTATTTTG AGGGCCTGGC GGAGCAGGTT GATCCTCCTG TGCCGATACA GTCTCCATAT
    CTGCCCATCT ATTTTGGAAA TGTGTGTCTC CGATTCCTCC CAGTGTTTGA TATAGTAATC
    CACAGGTTTT TAGAGTTGCT CCCGGTATCC AAATCACTGG AGACTCTACT AGATCACCTG
    GGAGGCTTAT ATAAATTTCA TGATCGTCCA GTGACTTATT TGTATAATAC TCTGCATTAT
    TATGAAATGC ACCTGAGAAA TCGCGAAAGT CTCAAACGAA AACTTGTCCA TGCAATCATT
    GGGTCGCTCA AGGATAACCG ACCACAGGGC TGGTGTCTAA GTGACACTTA CCTTAAAAAT
    GCTATGAATG CACGAGAAGA CAATCCCTGG GTCCCAGACG ACTCTTACTA CTGCAAATTG
    ATTGGCAGAC TGGTAGATAC GATGGCTGGC AAATCTCCTG GTCCATTCCC AAACTGTGAC
    TGGAGGTTCA ATGAGTTTCC CAACCCCGCT GCCCATGCAC TGCATGTTAC TTGTGTGGAA
    CTCATGGCCT TGGCTGTTTC CGGCCAGGAT GTTGGAAATG CTCTTCTAAA TGTTGTCCTG
    AAGAGTCAGC CTTTAGTGCC AAGAGAGAAC ATTACAGCAT GGATGAATGC CATTGGATTG
    ATCATCACTG CCCTACCAGC TTTTTCTTGG TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01658
    Clone ID Related Accession (Same CDS sequence) NM_001205762.1
    Accession Version NM_001205762.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4107bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2011-10-23
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 23
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03047816.1 and AAFC03075095.1. On Apr 20, 2011 this sequence version replaced XM_591317.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGACGC AACTACAGAG CATTTTTGAG GAGGTGGTGA AAACGGAGAT TATAGAAGAG 
    GCTTTCCCTG GCATGTTTAT GGACACCCCT GAAGATGAAA AAACAAAACT TATTAGCTGT
    TTAGGGGCCT TCAGACAATT TTGGGCTGGT CTTTCTCAGG AATCTCATGA ACAGTGCATC
    CAGTGGATTG TTAAATTCAT TCATGGCCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT
    GACTGCTTAG CAATGGCAGT CGAGACTGGT CTCCTTCCAC CCAGGATGGT TTGTGAATCC
    CTGATAAACT CTGACACACT TGAATGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA
    CTGGTTCGGA AAATAATTGG AGGGGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG
    ATTTTGGAGA AAATCTTGAC AATTCCCAAT ACAGTGAGCT CAGCTGTTGT ACAGCAGCTT
    TTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCTTGTTT ACTACCAGCA
    TATTTTGCAG TCACAGAGAT CAGGAAACTA TATCCTGAGG GAAAACTTCC ACATTGGTTA
    CTTGGAAACC TGGTATCAGA CTTTGTGGAT ACCTTCAGGC CAACAGCAAG GATAAACTCC
    ATTTGTGGCC GTTGCAGCCT TCTACCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA
    TGGAAATTGG ACCCTACAAC TCTCCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG
    GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTTT TGGAGCAGCC TTATTCCAGG
    GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG
    GAGGACCAGT TGGTGGACCT GGTGGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG
    TTTGATGATG GGGGAACAAG CCAACTCCTC TGGCAGCATC TCTCGAGTCA GCTCATTTTC
    TTTGTGCTTT TCCAGTTTGC AAGTTTCCCA CATATGGTCC TTTCTCTTCA TCAGAAGTTG
    GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCTT GCAATTCATT
    TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTTC CTGTCATGAA GCTCTTCGAC
    CTTCTGTACC CAGAAAAAGA ATTTATCCCA GTTCCTGACA TTAACAAACC CCAGTCAACT
    CATGCCTTTG CCATGACCTG CATTTGGATT CACCTCAACA GAAAAGCCCA AAATGACAAC
    TCCAAACTAC AGATTCCAAT ACCTCACTCC CTAAAGCTTC ACCATGAGTT CCTGCAGCAG
    AGTCTTAGAA ATAAGAGTTT ACAAATGAAT GACTATAAGA TCGCCCTGCT ATGTAATGCA
    TACTCTACAA ATTCAGAATG TTTTACGTTA CCTATGGGAG CTCTGGTGGA GACTATTTAT
    GGAAATGGAA TTATGAGGAT ACCACTTCCT GGAACGAGCT GTTTGGCTTC AGGATCTATT
    ACGCCCTTAC CAATGAACCT CCTGGATTCA CTGACGGTTC ATGCCAAAAT GAGCCTTATC
    CACAGCATTG CTACCAGAGT GATAAAACTT GCTCACGCAA AGTCCAGTGT GGCCTTGGCT
    CCAGCCCTAG TGGAAACTTA CAGCCGTTTA TTGGTCTATA TGGAAATAGA ATCTTTGGGC
    ATCAAAGGAT TTATCAGTCA GCTTCTGCCA ACTGTTTTTA AGTCTCATGC CTGGGGTATC
    TTGCATACGC TTCTTGAGAT GTTCAGCTAC CGGATGCACC ACATTCAGCC CCATTACAGA
    GTTCAGCTCC TAAGTCATCT TCATACTTTG GCTGCAGTTG CACAGACAAA CCAGAACCAG
    CTCCATCTCT GTGTTGAGAG TACTGCACTC AGGCTTATAA CAGCACTAGG TAGCTCAGAA
    GTACAGCCAC AGTTTACACG CTTCCTCAGT GATCCCAAGA CGGTGCTCTC AGCGGAATCC
    GAAGAACTGA ACCGTGCCTT GATACTGACC TTAGCTAGAG CAACTCACGT AACAGATTTT
    TTCACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG
    AGTTTTACAC CTCACAATTG GGCTTCACAT ACTCTTAGTT GTTTTCCAGG CCCACTACAG
    GCATTCTTTA AACAAAATAA TGTACCTCAA GAGAGTCGTT TTAATCTGAA AAAAAATGTG
    GAGGAGGAGT ATAGGAAGTG GAAGTCAATG ACCAACGAAA ATGACATCAT CACCCACTTT
    TCTGCACAAC GTTCTCCTCC TCTCTTCCTT TGTCTGCTCT GGAAAATGCT CCTGGAAACA
    GATCACATTA ATCAGATTGG CTATAGAGTA CTGGAGAGAA TTGGAGCCAG GGCCTTAGTA
    GTCCATGTGA GAACTTTTGC AGATTTCCTG GTATATGAGT TCTCTACGTC AGCTGGAGGT
    CAGCAACTCA ACAAATGCAT TGAAATTCTT AATGATATGG TATGGAAGTA CAACATTGTT
    ACACTGGACA GATTAATTCT CTGCCTGGCC ATGCGTAGCC ACGAAGGGAA TGAAGCCCAA
    GTTTGTTATT TCATAATTCA GTTGCTGTTA CTCAAGCCAA ATGATTTTAG AAATCGAGTA
    AGTGACTTTG TGAAGGAAAA TTCCCCAGAA CACTGGCTAC AGAATGACTG GCACACCAAG
    CACATGAATT ATCACAAGAA ATACCCGGAG AAGCTCTATT TTGAGGGCCT GGCGGAGCAG
    GTTGATCCTC CTGTGCCGAT ACAGTCTCCA TATCTGCCCA TCTATTTTGG AAATGTGTGT
    CTCCGATTCC TCCCAGTGTT TGATATAGTA ATCCACAGGT TTTTAGAGTT GCTCCCGGTA
    TCCAAATCAC TGGAGACTCT ACTAGATCAC CTGGGAGGCT TATATAAATT TCATGATCGT
    CCAGTGACTT ATTTGTATAA TACTCTGCAT TATTATGAAA TGCACCTGAG AAATCGCGAA
    AGTCTCAAAC GAAAACTTGT CCATGCAATC ATTGGGTCGC TCAAGGATAA CCGACCACAG
    GGCTGGTGTC TAAGTGACAC TTACCTTAAA AATGCTATGA ATGCACGAGA AGACAATCCC
    TGGGTCCCAG ACGACTCTTA CTACTGCAAA TTGATTGGCA GACTGGTAGA TACGATGGCT
    GGCAAATCTC CTGGTCCATT CCCAAACTGT GACTGGAGGT TCAATGAGTT TCCCAACCCC
    GCTGCCCACG CACTGCATGT TACTTGTGTG GAACTCATGG CCTTGGCTGT TTCCGGCCAG
    GATGTTGGAA ATGCTCTTCT AAATGTTGTC CTGAAGAGTC AGCCTTTAGT GCCAAGAGAG
    AACATTACAG CATGGATGAA TGCCATTGGA TTGATCATCA CTGCCCTACC AGAGCCGTAT
    TGGATTGTCC TTCACGATCG AATAGTGAGT GTTATCAGCA GTCCCAGCTT GACGTCTGAG
    ACAGAGTGGG TCGGCTATCC TTTCCGGCTC TTCGATTTCA CCGCTTGCCA TCAGTCCTAC
    TCTGAGATGA GCTGTAGCTA CACGTTAGCT CTGGCACATG CAGTGTGGCA CCACTCCAGC
    ATCGGGCAGC TTTCTCTCAT TCCCAAATTT CTCACTGAAG TACTTCTTCC TATAGTGAAG
    ACTGAGTTCC AGTTGCTTTA TGTGTACCAC CTTGTGGGAC CATTTTTACA AAGGTTTCAA
    CAAGAGAGAA CTCGATGTAT GATAGAGATT GGTGTGGCGT TTTATGACAT GTTGCTGAAC
    GTAGACCAGT GTAGCACCCA CTTAAACTAC ATGGATCCCA TCTGTGACTT CCTGTATCAT
    ATGAAGTATA TGTTTACTGG TGACAGTGTA AAAGAACAAG TAGAAAAGAT TATCTGTAAT
    TTAAAACCGG CTTTAAAACT CCGTCTTCGA TTCATCACTC ACATTAGCAA GATGGAACCG
    GCTGCCGTGC CTCCGCAAGC CGTCAGCAGT GGGTCTCCAG CACCTCAGTC TACTCAGGTG
    CCAGGGTCTC TGCCGGTGAC TCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192691.1
    CDS1..4107
    Translation

    Target ORF information:

    RefSeq Version NM_001205762.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 23 (MED23), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205762.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGACGC AACTACAGAG CATTTTTGAG GAGGTGGTGA AAACGGAGAT TATAGAAGAG 
    GCTTTCCCTG GCATGTTTAT GGACACCCCT GAAGATGAAA AAACAAAACT TATTAGCTGT
    TTAGGGGCCT TCAGACAATT TTGGGCTGGT CTTTCTCAGG AATCTCATGA ACAGTGCATC
    CAGTGGATTG TTAAATTCAT TCATGGCCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT
    GACTGCTTAG CAATGGCAGT CGAGACTGGT CTCCTTCCAC CCAGGATGGT TTGTGAATCC
    CTGATAAACT CTGACACACT TGAATGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA
    CTGGTTCGGA AAATAATTGG AGGGGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG
    ATTTTGGAGA AAATCTTGAC AATTCCCAAT ACAGTGAGCT CAGCTGTTGT ACAGCAGCTT
    TTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCTTGTTT ACTACCAGCA
    TATTTTGCAG TCACAGAGAT CAGGAAACTA TATCCTGAGG GAAAACTTCC ACATTGGTTA
    CTTGGAAACC TGGTATCAGA CTTTGTGGAT ACCTTCAGGC CAACAGCAAG GATAAACTCC
    ATTTGTGGCC GTTGCAGCCT TCTACCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA
    TGGAAATTGG ACCCTACAAC TCTCCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG
    GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTTT TGGAGCAGCC TTATTCCAGG
    GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG
    GAGGACCAGT TGGTGGACCT GGTGGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG
    TTTGATGATG GGGGAACAAG CCAACTCCTC TGGCAGCATC TCTCGAGTCA GCTCATTTTC
    TTTGTGCTTT TCCAGTTTGC AAGTTTCCCA CATATGGTCC TTTCTCTTCA TCAGAAGTTG
    GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCTT GCAATTCATT
    TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTTC CTGTCATGAA GCTCTTCGAC
    CTTCTGTACC CAGAAAAAGA ATTTATCCCA GTTCCTGACA TTAACAAACC CCAGTCAACT
    CATGCCTTTG CCATGACCTG CATTTGGATT CACCTCAACA GAAAAGCCCA AAATGACAAC
    TCCAAACTAC AGATTCCAAT ACCTCACTCC CTAAAGCTTC ACCATGAGTT CCTGCAGCAG
    AGTCTTAGAA ATAAGAGTTT ACAAATGAAT GACTATAAGA TCGCCCTGCT ATGTAATGCA
    TACTCTACAA ATTCAGAATG TTTTACGTTA CCTATGGGAG CTCTGGTGGA GACTATTTAT
    GGAAATGGAA TTATGAGGAT ACCACTTCCT GGAACGAGCT GTTTGGCTTC AGGATCTATT
    ACGCCCTTAC CAATGAACCT CCTGGATTCA CTGACGGTTC ATGCCAAAAT GAGCCTTATC
    CACAGCATTG CTACCAGAGT GATAAAACTT GCTCACGCAA AGTCCAGTGT GGCCTTGGCT
    CCAGCCCTAG TGGAAACTTA CAGCCGTTTA TTGGTCTATA TGGAAATAGA ATCTTTGGGC
    ATCAAAGGAT TTATCAGTCA GCTTCTGCCA ACTGTTTTTA AGTCTCATGC CTGGGGTATC
    TTGCATACGC TTCTTGAGAT GTTCAGCTAC CGGATGCACC ACATTCAGCC CCATTACAGA
    GTTCAGCTCC TAAGTCATCT TCATACTTTG GCTGCAGTTG CACAGACAAA CCAGAACCAG
    CTCCATCTCT GTGTTGAGAG TACTGCACTC AGGCTTATAA CAGCACTAGG TAGCTCAGAA
    GTACAGCCAC AGTTTACACG CTTCCTCAGT GATCCCAAGA CGGTGCTCTC AGCGGAATCC
    GAAGAACTGA ACCGTGCCTT GATACTGACC TTAGCTAGAG CAACTCACGT AACAGATTTT
    TTCACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG
    AGTTTTACAC CTCACAATTG GGCTTCACAT ACTCTTAGTT GTTTTCCAGG CCCACTACAG
    GCATTCTTTA AACAAAATAA TGTACCTCAA GAGAGTCGTT TTAATCTGAA AAAAAATGTG
    GAGGAGGAGT ATAGGAAGTG GAAGTCAATG ACCAACGAAA ATGACATCAT CACCCACTTT
    TCTGCACAAC GTTCTCCTCC TCTCTTCCTT TGTCTGCTCT GGAAAATGCT CCTGGAAACA
    GATCACATTA ATCAGATTGG CTATAGAGTA CTGGAGAGAA TTGGAGCCAG GGCCTTAGTA
    GTCCATGTGA GAACTTTTGC AGATTTCCTG GTATATGAGT TCTCTACGTC AGCTGGAGGT
    CAGCAACTCA ACAAATGCAT TGAAATTCTT AATGATATGG TATGGAAGTA CAACATTGTT
    ACACTGGACA GATTAATTCT CTGCCTGGCC ATGCGTAGCC ACGAAGGGAA TGAAGCCCAA
    GTTTGTTATT TCATAATTCA GTTGCTGTTA CTCAAGCCAA ATGATTTTAG AAATCGAGTA
    AGTGACTTTG TGAAGGAAAA TTCCCCAGAA CACTGGCTAC AGAATGACTG GCACACCAAG
    CACATGAATT ATCACAAGAA ATACCCGGAG AAGCTCTATT TTGAGGGCCT GGCGGAGCAG
    GTTGATCCTC CTGTGCCGAT ACAGTCTCCA TATCTGCCCA TCTATTTTGG AAATGTGTGT
    CTCCGATTCC TCCCAGTGTT TGATATAGTA ATCCACAGGT TTTTAGAGTT GCTCCCGGTA
    TCCAAATCAC TGGAGACTCT ACTAGATCAC CTGGGAGGCT TATATAAATT TCATGATCGT
    CCAGTGACTT ATTTGTATAA TACTCTGCAT TATTATGAAA TGCACCTGAG AAATCGCGAA
    AGTCTCAAAC GAAAACTTGT CCATGCAATC ATTGGGTCGC TCAAGGATAA CCGACCACAG
    GGCTGGTGTC TAAGTGACAC TTACCTTAAA AATGCTATGA ATGCACGAGA AGACAATCCC
    TGGGTCCCAG ACGACTCTTA CTACTGCAAA TTGATTGGCA GACTGGTAGA TACGATGGCT
    GGCAAATCTC CTGGTCCATT CCCAAACTGT GACTGGAGGT TCAATGAGTT TCCCAACCCC
    GCTGCCCACG CACTGCATGT TACTTGTGTG GAACTCATGG CCTTGGCTGT TTCCGGCCAG
    GATGTTGGAA ATGCTCTTCT AAATGTTGTC CTGAAGAGTC AGCCTTTAGT GCCAAGAGAG
    AACATTACAG CATGGATGAA TGCCATTGGA TTGATCATCA CTGCCCTACC AGAGCCGTAT
    TGGATTGTCC TTCACGATCG AATAGTGAGT GTTATCAGCA GTCCCAGCTT GACGTCTGAG
    ACAGAGTGGG TCGGCTATCC TTTCCGGCTC TTCGATTTCA CCGCTTGCCA TCAGTCCTAC
    TCTGAGATGA GCTGTAGCTA CACGTTAGCT CTGGCACATG CAGTGTGGCA CCACTCCAGC
    ATCGGGCAGC TTTCTCTCAT TCCCAAATTT CTCACTGAAG TACTTCTTCC TATAGTGAAG
    ACTGAGTTCC AGTTGCTTTA TGTGTACCAC CTTGTGGGAC CATTTTTACA AAGGTTTCAA
    CAAGAGAGAA CTCGATGTAT GATAGAGATT GGTGTGGCGT TTTATGACAT GTTGCTGAAC
    GTAGACCAGT GTAGCACCCA CTTAAACTAC ATGGATCCCA TCTGTGACTT CCTGTATCAT
    ATGAAGTATA TGTTTACTGG TGACAGTGTA AAAGAACAAG TAGAAAAGAT TATCTGTAAT
    TTAAAACCGG CTTTAAAACT CCGTCTTCGA TTCATCACTC ACATTAGCAA GATGGAACCG
    GCTGCCGTGC CTCCGCAAGC CGTCAGCAGT GGGTCTCCAG CACCTCAGTC TACTCAGGTG
    CCAGGGTCTC TGCCGGTGAC TCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.