MED23 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED23
Entrez Gene ID	9439
Full Name	mediator complex subunit 23
Synonyms	ARC130,CRSP130,CRSP133,CRSP3,DRIP130,MRT18,SUR-2,SUR2
General protein information	Preferred Names mediator complex subunit 23 Names mediator of RNA polymerase II transcription subunit 23 130 kDa transcriptional co-activator 133 kDa transcriptional co-activator activator-recruited cofactor 130 kDa component cofactor required for Sp1 transcriptional activation subunit 3 vitamin D3 receptor interacting protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q23.2
Summary	The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2012].
Disorder MIM:	605042
Disorder Html:	Mental retardation, autosomal recessive 18, 614249 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011536257.2	XP_011534559.1	mediator of RNA polymerase II transcription subunit 23 isoform X2
XM_005267223.3	XP_005267280.1	mediator of RNA polymerase II transcription subunit 23 isoform X1
NM_001270521.1	NP_001257450.1	mediator of RNA polymerase II transcription subunit 23 isoform c
NM_001376518.1	NP_001363447.1	mediator of RNA polymerase II transcription subunit 23 isoform f
NM_001376520.1	NP_001363449.1	mediator of RNA polymerase II transcription subunit 23 isoform h
NM_001376524.1	NP_001363453.1	mediator of RNA polymerase II transcription subunit 23 isoform l
NM_001376517.1	NP_001363446.1	mediator of RNA polymerase II transcription subunit 23 isoform e
XM_006715612.3	XP_006715675.1	mediator of RNA polymerase II transcription subunit 23 isoform X4
XM_006715612.2	XP_006715675.1	mediator of RNA polymerase II transcription subunit 23 isoform X4
NM_001376519.1	NP_001363448.1	mediator of RNA polymerase II transcription subunit 23 isoform g
NM_001270521.2	NP_001257450.1	mediator of RNA polymerase II transcription subunit 23 isoform c
NM_015979.4	NP_057063.2	mediator of RNA polymerase II transcription subunit 23 isoform b
NM_004830.4	NP_004821.2	mediator of RNA polymerase II transcription subunit 23 isoform a
NM_004830.3	NP_004821.2	mediator of RNA polymerase II transcription subunit 23 isoform a
XM_005267223.2	XP_005267280.1	mediator of RNA polymerase II transcription subunit 23 isoform X1
NM_001270522.1	NP_001257451.1	mediator of RNA polymerase II transcription subunit 23 isoform d
NM_001376522.1	NP_001363451.1	mediator of RNA polymerase II transcription subunit 23 isoform j
NM_001376521.1	NP_001363450.1	mediator of RNA polymerase II transcription subunit 23 isoform i
NM_001270522.2	NP_001257451.1	mediator of RNA polymerase II transcription subunit 23 isoform d
NM_001376523.1	NP_001363452.1	mediator of RNA polymerase II transcription subunit 23 isoform k
XM_011536257.3	XP_011534559.1	mediator of RNA polymerase II transcription subunit 23 isoform X2
NM_015979.3	NP_057063.2	mediator of RNA polymerase II transcription subunit 23 isoform b
XM_017011501.1	XP_016866990.1	mediator of RNA polymerase II transcription subunit 23 isoform X3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Med23	NP_001159888.1
Gallus gallus (chicken)	MED23	XP_424537.3
Caenorhabditis elegans (roundworm)	sur-2	NP_493575.2
Bos taurus (cattle)	MED23	NP_001192691.1
Homo sapiens (human)	MED23	NP_004821.2
Rattus norvegicus (Norway rat)	Med23	NP_001263983.1
Pan troglodytes (chimpanzee)	MED23	XP_527503.3
Canis lupus familiaris (dog)	MED23	XP_005627145.1
	MED23	XP_002803940.1
Danio rerio (zebrafish)	med23	NP_001003990.1
Drosophila melanogaster (fruit fly)	MED23	NP_001137741.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006046	XP_316095.4
Xenopus tropicalis (tropical clawed frog)	med23	XP_002936353.2

Gene Ontology
Bibliography

Related articles in PubMed

Effect of l-arginine, asymmetric dimethylarginine, and symmetric dimethylarginine on ischemic heart disease risk: A Mendelian randomization study.
Au Yeung SL, Lin SL, Lam HS, Schooling CM
American heart journal18254-61(2016 Dec)

MED23-associated refractory epilepsy successfully treated with the ketogenic diet.
Lionel AC, Monfared N, Scherer SW, Marshall CR, Mercimek-Mahmutoglu S
American journal of medical genetics. Part A170(9)2421-5(2016 Sep)

A 7-Gene Signature Depicts the Biochemical Profile of Early Prefibrotic Myelofibrosis.
Skov V, Burton M, Thomassen M, Stauffer Larsen T, Riley CH, Brinch Madelung A, Kj?r L, Bondo H, Stamp I, Ehinger M, Dahl-S?rensen R, Brochmann N, Nielsen K, Thiele J, Jensen MK, Weis Bjerrum O, Kruse TA, Hasselbalch HC
PloS one11(8)e0161570(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Higher l-arginine was associated with higher risk of Ischemic heart disease (odds ratio and of myocardial infarction, based on 2 SNPs from MED23.
Title: Effect of l-arginine, asymmetric dimethylarginine, and symmetric dimethylarginine on ischemic heart disease risk: A Mendelian randomization study.

This is the first patient with documented refractory epilepsy caused by a novel homozygous pathogenic variant in MED23 expanding the phenotypic spectrum. Identification of the underlying genetic defect in MED23 sheds light on the possible mechanism of complete response to the ketogenic diet in this child.
Title: MED23-associated refractory epilepsy successfully treated with the ketogenic diet.

a 7-gene signature was identified which correctly predicted the primary prefibrotic myelofibrosis group with a sensitivity of 100% and a specificity of 89%. The 7 genes included MPO, CEACAM8, CRISP3, MS4A3, CEACAM6, HEMGN, and MMP8
Title: A 7-Gene Signature Depicts the Biochemical Profile of Early Prefibrotic Myelofibrosis.

In Title.
Title: Mutational Heterogeneity of MED23 Gene in Colorectal Cancers.

MED23-associated intellectual disability has been found in two brothers from a non-consanguineous family.
Title: MED23-associated intellectual disability in a non-consanguineous family.

The following MED23 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED23 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu41026	XM_006715612.3 Latest version!	Homo sapiens mediator complex subunit 23 (MED23), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu96920	XM_017011501.1 Latest version!	Homo sapiens mediator complex subunit 23 (MED23), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
OHu04644	NM_004830.4 Latest version!	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$727.30 $1039.00
OHu109117	NM_001376518.1 Latest version!	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu109121	NM_001376522.1 Latest version!	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu109126	NM_001376521.1 Latest version!	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu109129	NM_001376520.1 Latest version!	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu109130	NM_001376524.1 Latest version!	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu109131	NM_001376519.1 Latest version!	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu109136	NM_001376523.1 Latest version!	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu41025	NM_001376517.1 Latest version!	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu03689	NM_001270521.2 Latest version!	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu03690	NM_001270522.2 Latest version!	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu19949	NM_015979.4 Latest version!	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu41025	XM_005267223.3 Latest version!	Homo sapiens mediator complex subunit 23 (MED23), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu62822	XM_011536257.3 Latest version!	Homo sapiens mediator complex subunit 23 (MED23), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu41026	XM_006715612.2	Homo sapiens mediator complex subunit 23 (MED23), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu03689	NM_001270521.1	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu04644	NM_004830.3	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$727.30 $1039.00
OHu41025	XM_005267223.2	Homo sapiens mediator complex subunit 23 (MED23), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu03690	NM_001270522.1	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu62822	XM_011536257.2	Homo sapiens mediator complex subunit 23 (MED23), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu19949	NM_015979.3	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED23 ( XM_006715612.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006715675.1 Homo sapiens mediator complex subunit 23 (MED23), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu41026
Clone ID Related Accession (Same CDS sequence)	XM_006715612.3 , XM_006715612.2
Accession Version	XM_006715612.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006715612.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGACCT TGAACATTGC TCAGCACAAG 
CAGCGCTGCC CTGTGCTGGA GGACCAGTTG GTGGATCTGG TTGTTTATGC CATGGAGCGA 
TCTGAGACCG AGGAGAAGTT TGACGATGGG GGAACAAGCC AACTCCTGTG GCAGCATCTC 
TCAAGTCAGC TCATTTTCTT TGTGCTTTTC CAGTTTGCAA GTTTTCCACA TATGGTGCTT 
TCTCTTCATC AGAAGTTAGC AGGGCGAGGA CTGATTAAAG GCAGAGATCA TCTTATGTGG 
GTTCTCCTGC AATTCATTTC TGGAAGTATT CAGAAAAATG CACTAGCTGA TTTTCTCCCT 
GTGATGAAGC TCTTCGACTT GCTATACCCA GAAAAAGAAT ATATCCCAGT TCCTGATATT 
AACAAACCCC AGTCAACCCA TGCCTTTGCA ATGACCTGTA TTTGGATTCA TCTCAATAGA 
AAAGCTCAAA ATGACAACTC CAAGCTACAG ATTCCAATAC CTCATTCCCT AAGACTTCAC 
CATGAGTTCC TGCAGCAGAG TCTAAGAAAT AAAAGTTTAC AGATGAATGA CTATAAGATT 
GCTCTATTGT GTAATGCATA CTCTACAAAT TCAGAATGTT TTACATTACC CATGGGAGCT 
CTGGTAGAAA CTATTTATGG AAATGGAATT ATGAGGATAC CTCTCCCTGG AACAAACTGT 
ATGGCTTCAG GATCTATTAC CCCCTTACCT ATGAACCTCC TGGATTCACT GACAGTTCAT 
GCCAAAATGA GCCTTATTCA CAGCATTGCA ACCAGGGTGA TAAAACTTGC TCATGCAAAG 
TCCAGTGTGG CCTTGGCTCC AGCCCTAGTG GAAACTTACA GTCGTTTATT GGTCTATATG 
GAAATAGAGT CTTTGGGCAT CAAAGGATTT ATCAGTCAGC TTTTGCCAAC TGTTTTCAAA 
TCACATGCAT GGGGGATCTT ACACACACTC CTTGAGATGT TTAGCTACCG GATGCATCAT 
ATTCAGCCTC ATTACAGAGT TCAGCTCCTG AGTCATCTTC ATACTTTGGC TGCAGTTGCA 
CAAACAAACC AGAACCAGCT CCATCTTTGT GTCGAGAGCA CTGCTCTCAG GCTTATAACA 
GCATTAGGTA GCTCAGAGGT ACAACCGCAG TTTACACGCT TCCTTAGTGA TCCCAAAACA 
GTGCTCTCAG CAGAATCTGA AGAACTGAAC CGAGCCTTGA TATTGACCTT GGCTAGAGCA 
ACTCATGTAA CAGATTTTTT TACAGGCTCT GATTCAATTC AGGGAACTTG GTGTAAAGAC 
ATACTTCAGA CCATCATGAG TTTCACTCCT CATAATTGGG CTTCACACAC CCTGAGCTGT 
TTTCCAGGCC CACTACAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006715675.1
CDS	183..2426
Translation	METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQTLNIAQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQN

Target ORF information:

RefSeq Version	XM_006715612.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006715612.3

ORF Insert Sequence:

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGACCT TGAACATTGC TCAGCACAAG 
CAGCGCTGCC CTGTGCTGGA GGACCAGTTG GTGGATCTGG TTGTTTATGC CATGGAGCGA 
TCTGAGACCG AGGAGAAGTT TGACGATGGG GGAACAAGCC AACTCCTGTG GCAGCATCTC 
TCAAGTCAGC TCATTTTCTT TGTGCTTTTC CAGTTTGCAA GTTTTCCACA TATGGTGCTT 
TCTCTTCATC AGAAGTTAGC AGGGCGAGGA CTGATTAAAG GCAGAGATCA TCTTATGTGG 
GTTCTCCTGC AATTCATTTC TGGAAGTATT CAGAAAAATG CACTAGCTGA TTTTCTCCCT 
GTGATGAAGC TCTTCGACTT GCTATACCCA GAAAAAGAAT ATATCCCAGT TCCTGATATT 
AACAAACCCC AGTCAACCCA TGCCTTTGCA ATGACCTGTA TTTGGATTCA TCTCAATAGA 
AAAGCTCAAA ATGACAACTC CAAGCTACAG ATTCCAATAC CTCATTCCCT AAGACTTCAC 
CATGAGTTCC TGCAGCAGAG TCTAAGAAAT AAAAGTTTAC AGATGAATGA CTATAAGATT 
GCTCTATTGT GTAATGCATA CTCTACAAAT TCAGAATGTT TTACATTACC CATGGGAGCT 
CTGGTAGAAA CTATTTATGG AAATGGAATT ATGAGGATAC CTCTCCCTGG AACAAACTGT 
ATGGCTTCAG GATCTATTAC CCCCTTACCT ATGAACCTCC TGGATTCACT GACAGTTCAT 
GCCAAAATGA GCCTTATTCA CAGCATTGCA ACCAGGGTGA TAAAACTTGC TCATGCAAAG 
TCCAGTGTGG CCTTGGCTCC AGCCCTAGTG GAAACTTACA GTCGTTTATT GGTCTATATG 
GAAATAGAGT CTTTGGGCAT CAAAGGATTT ATCAGTCAGC TTTTGCCAAC TGTTTTCAAA 
TCACATGCAT GGGGGATCTT ACACACACTC CTTGAGATGT TTAGCTACCG GATGCATCAT 
ATTCAGCCTC ATTACAGAGT TCAGCTCCTG AGTCATCTTC ATACTTTGGC TGCAGTTGCA 
CAAACAAACC AGAACCAGCT CCATCTTTGT GTCGAGAGCA CTGCTCTCAG GCTTATAACA 
GCATTAGGTA GCTCAGAGGT ACAACCGCAG TTTACACGCT TCCTTAGTGA TCCCAAAACA 
GTGCTCTCAG CAGAATCTGA AGAACTGAAC CGAGCCTTGA TATTGACCTT GGCTAGAGCA 
ACTCATGTAA CAGATTTTTT TACAGGCTCT GATTCAATTC AGGGAACTTG GTGTAAAGAC 
ATACTTCAGA CCATCATGAG TTTCACTCCT CATAATTGGG CTTCACACAC CCTGAGCTGT 
TTTCCAGGCC CACTACAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( XM_006715612.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006715675.1 Homo sapiens mediator complex subunit 23 (MED23), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu41026
Clone ID Related Accession (Same CDS sequence)	XM_006715612.3 , XM_006715612.2
Accession Version	XM_006715612.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006715612.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGACCT TGAACATTGC TCAGCACAAG 
CAGCGCTGCC CTGTGCTGGA GGACCAGTTG GTGGATCTGG TTGTTTATGC CATGGAGCGA 
TCTGAGACCG AGGAGAAGTT TGACGATGGG GGAACAAGCC AACTCCTGTG GCAGCATCTC 
TCAAGTCAGC TCATTTTCTT TGTGCTTTTC CAGTTTGCAA GTTTTCCACA TATGGTGCTT 
TCTCTTCATC AGAAGTTAGC AGGGCGAGGA CTGATTAAAG GCAGAGATCA TCTTATGTGG 
GTTCTCCTGC AATTCATTTC TGGAAGTATT CAGAAAAATG CACTAGCTGA TTTTCTCCCT 
GTGATGAAGC TCTTCGACTT GCTATACCCA GAAAAAGAAT ATATCCCAGT TCCTGATATT 
AACAAACCCC AGTCAACCCA TGCCTTTGCA ATGACCTGTA TTTGGATTCA TCTCAATAGA 
AAAGCTCAAA ATGACAACTC CAAGCTACAG ATTCCAATAC CTCATTCCCT AAGACTTCAC 
CATGAGTTCC TGCAGCAGAG TCTAAGAAAT AAAAGTTTAC AGATGAATGA CTATAAGATT 
GCTCTATTGT GTAATGCATA CTCTACAAAT TCAGAATGTT TTACATTACC CATGGGAGCT 
CTGGTAGAAA CTATTTATGG AAATGGAATT ATGAGGATAC CTCTCCCTGG AACAAACTGT 
ATGGCTTCAG GATCTATTAC CCCCTTACCT ATGAACCTCC TGGATTCACT GACAGTTCAT 
GCCAAAATGA GCCTTATTCA CAGCATTGCA ACCAGGGTGA TAAAACTTGC TCATGCAAAG 
TCCAGTGTGG CCTTGGCTCC AGCCCTAGTG GAAACTTACA GTCGTTTATT GGTCTATATG 
GAAATAGAGT CTTTGGGCAT CAAAGGATTT ATCAGTCAGC TTTTGCCAAC TGTTTTCAAA 
TCACATGCAT GGGGGATCTT ACACACACTC CTTGAGATGT TTAGCTACCG GATGCATCAT 
ATTCAGCCTC ATTACAGAGT TCAGCTCCTG AGTCATCTTC ATACTTTGGC TGCAGTTGCA 
CAAACAAACC AGAACCAGCT CCATCTTTGT GTCGAGAGCA CTGCTCTCAG GCTTATAACA 
GCATTAGGTA GCTCAGAGGT ACAACCGCAG TTTACACGCT TCCTTAGTGA TCCCAAAACA 
GTGCTCTCAG CAGAATCTGA AGAACTGAAC CGAGCCTTGA TATTGACCTT GGCTAGAGCA 
ACTCATGTAA CAGATTTTTT TACAGGCTCT GATTCAATTC AGGGAACTTG GTGTAAAGAC 
ATACTTCAGA CCATCATGAG TTTCACTCCT CATAATTGGG CTTCACACAC CCTGAGCTGT 
TTTCCAGGCC CACTACAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006715675.1
CDS	187..2430
Translation	METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQTLNIAQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQN

Target ORF information:

RefSeq Version	XM_006715612.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006715612.2

ORF Insert Sequence:

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGACCT TGAACATTGC TCAGCACAAG 
CAGCGCTGCC CTGTGCTGGA GGACCAGTTG GTGGATCTGG TTGTTTATGC CATGGAGCGA 
TCTGAGACCG AGGAGAAGTT TGACGATGGG GGAACAAGCC AACTCCTGTG GCAGCATCTC 
TCAAGTCAGC TCATTTTCTT TGTGCTTTTC CAGTTTGCAA GTTTTCCACA TATGGTGCTT 
TCTCTTCATC AGAAGTTAGC AGGGCGAGGA CTGATTAAAG GCAGAGATCA TCTTATGTGG 
GTTCTCCTGC AATTCATTTC TGGAAGTATT CAGAAAAATG CACTAGCTGA TTTTCTCCCT 
GTGATGAAGC TCTTCGACTT GCTATACCCA GAAAAAGAAT ATATCCCAGT TCCTGATATT 
AACAAACCCC AGTCAACCCA TGCCTTTGCA ATGACCTGTA TTTGGATTCA TCTCAATAGA 
AAAGCTCAAA ATGACAACTC CAAGCTACAG ATTCCAATAC CTCATTCCCT AAGACTTCAC 
CATGAGTTCC TGCAGCAGAG TCTAAGAAAT AAAAGTTTAC AGATGAATGA CTATAAGATT 
GCTCTATTGT GTAATGCATA CTCTACAAAT TCAGAATGTT TTACATTACC CATGGGAGCT 
CTGGTAGAAA CTATTTATGG AAATGGAATT ATGAGGATAC CTCTCCCTGG AACAAACTGT 
ATGGCTTCAG GATCTATTAC CCCCTTACCT ATGAACCTCC TGGATTCACT GACAGTTCAT 
GCCAAAATGA GCCTTATTCA CAGCATTGCA ACCAGGGTGA TAAAACTTGC TCATGCAAAG 
TCCAGTGTGG CCTTGGCTCC AGCCCTAGTG GAAACTTACA GTCGTTTATT GGTCTATATG 
GAAATAGAGT CTTTGGGCAT CAAAGGATTT ATCAGTCAGC TTTTGCCAAC TGTTTTCAAA 
TCACATGCAT GGGGGATCTT ACACACACTC CTTGAGATGT TTAGCTACCG GATGCATCAT 
ATTCAGCCTC ATTACAGAGT TCAGCTCCTG AGTCATCTTC ATACTTTGGC TGCAGTTGCA 
CAAACAAACC AGAACCAGCT CCATCTTTGT GTCGAGAGCA CTGCTCTCAG GCTTATAACA 
GCATTAGGTA GCTCAGAGGT ACAACCGCAG TTTACACGCT TCCTTAGTGA TCCCAAAACA 
GTGCTCTCAG CAGAATCTGA AGAACTGAAC CGAGCCTTGA TATTGACCTT GGCTAGAGCA 
ACTCATGTAA CAGATTTTTT TACAGGCTCT GATTCAATTC AGGGAACTTG GTGTAAAGAC 
ATACTTCAGA CCATCATGAG TTTCACTCCT CATAATTGGG CTTCACACAC CCTGAGCTGT 
TTTCCAGGCC CACTACAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( XM_017011501.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866990.1 Homo sapiens mediator complex subunit 23 (MED23), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu96920
Clone ID Related Accession (Same CDS sequence)	XM_017011501.1
Accession Version	XM_017011501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGAT CTGAGACCGA GGAGAAGTTT GACGATGGGG GAACAAGCCA ACTCCTGTGG 
CAGCATCTCT CAAGTCAGCT CATTTTCTTT GTGCTTTTCC AGTTTGCAAG TTTTCCACAT 
ATGGTGCTTT CTCTTCATCA GAAGTTAGCA GGGCGAGGAC TGATTAAAGG CAGAGATCAT 
CTTATGTGGG TTCTCCTGCA ATTCATTTCT GGAAGTATTC AGAAAAATGC ACTAGCTGAT 
TTTCTCCCTG TGATGAAGCT CTTCGACTTG CTATACCCAG AAAAAGAATA TATCCCAGTT 
CCTGATATTA ACAAACCCCA GTCAACCCAT GCCTTTGCAA TGACCTGTAT TTGGATTCAT 
CTCAATAGAA AAGCTCAAAA TGACAACTCC AAGCTACAGA TTCCAATACC TCATTCCCTA 
AGACTTCACC ATGAGTTCCT GCAGCAGAGT CTAAGAAATA AAAGTTTACA GATGAATGAC 
TATAAGATTG CTCTATTGTG TAATGCATAC TCTACAAATT CAGAATGTTT TACATTACCC 
ATGGGAGCTC TGGTAGAAAC TATTTATGGA AATGGAATTA TGAGGATACC TCTCCCTGGA 
ACAAACTGTA TGGCTTCAGG ATCTATTACC CCCTTACCTA TGAACCTCCT GGATTCACTG 
ACAGTTCATG CCAAAATGAG CCTTATTCAC AGCATTGCAA CCAGGGTGAT AAAACTTGCT 
CATGCAAAGT CCAGTGTGGC CTTGGCTCCA GCCCTAGTGG AAACTTACAG TCGTTTATTG 
GTCTATATGG AAATAGAGTC TTTGGGCATC AAAGGATTTA TCAGTCAGCT TTTGCCAACT 
GTTTTCAAAT CACATGCATG GGGGATCTTA CACACACTCC TTGAGATGTT TAGCTACCGG 
ATGCATCATA TTCAGCCTCA TTACAGAGTT CAGCTCCTGA GTCATCTTCA TACTTTGGCT 
GCAGTTGCAC AAACAAACCA GAACCAGCTC CATCTTTGTG TCGAGAGCAC TGCTCTCAGG 
CTTATAACAG CATTAGGTAG CTCAGAGGTA CAACCGCAGT TTACACGCTT CCTTAGTGAT 
CCCAAAACAG TGCTCTCAGC AGAATCTGAA GAACTGAACC GAGCCTTGAT ATTGACCTTG 
GCTAGAGCAA CTCATGTAAC AGATTTTTTT ACAGGCTCTG ATTCAATTCA GGGAACTTGG 
TGTAAAGACA TACTTCAGAC CATCATGAGT TTCACTCCTC ATAATTGGGC TTCACACACC 
CTGAGCTGTT TTCCAGGCCC ACTACAGGCA TTCTTCAAAC AAAATAATGT GCCTCAGGAA 
AGCCGTTTTA ATCTGAAAAA AAATGTGGAG GAGGAGTATA GGAAGTGGAA GTCAATGAGC 
AACGAAAACG ACATTATTAC CCACTTCTCT ATGCAGGGCT CCCCTCCTCT CTTTCTTTGT 
CTTCTCTGGA AAATGCTCTT GGAAACAGAT CATATTAATC AGATTGGCTA TAGAGTATTA 
GAGAGAATTG GAGCCAGGGC CTTGGTAGCC CATGTGAGGA CATTTGCAGA TTTCCTGGTA 
TATGAGTTTT CTACATCAGC AGGGGGTCAG CAACTCAATA AATGCATTGA AATTCTTAAT 
GACATGGTAT GGAAGTATAA CATTGTTACA CTGGACAGAT TAATTCTCTG CCTGGCCATG 
CGTAGTCACG AAGGAAATGA AGCCCAGGTT TGTTATTTCA TAATTCAGTT GCTGTTACTC 
AAACCAAACG ATTTTAGAAA TCGAGTAAGT GACTTTGTGA AGGAAAATTC CCCAGAGCAC 
TGGTTACAGA ATGACTGGCA CACCAAGCAC ATGAATTATC ACAAGAAATA TCCAGAGAAG 
TTGTATTTTG AGGGCCTCGC GGAACAGGTG GATCCTCCTG TACAGATCCA GTCTCCCTAT 
CTGCCCATCT ATTTTGGGAA TGTGTGTCTT CGATTCCTTC CAGTATTTGA TATAGTAATC 
CACAGATTTT TAGAGTTGCT TCCGGTATCC AAATCACTGG AGACTCTACT GGATCATCTA 
GGAGGCTTAT ATAAATTTCA TGATCGTCCA GTGACTTATC TGTATAACAC TCTGCACTAT 
TATGAAATGC ACCTGAGAGA CCGCGCATTT CTCAAACGAA AACTCGTCCA TGCGATCATT 
GGCTCTCTGA AGGATAATCG ACCGCAGGGC TGGTGTCTAA GTGACACTTA CCTGAAATGC 
GCTATGAATG CACGAGAGGA AAATCCTTGG GTTCCAGATG ACACCTACTA TTGCAGATTG 
ATTGGCAGAC TAGTCGATAC GATGGCTGGC AAATCTCCTG GTCCCTTTCC AAACTGTGAC 
TGGAGATTCA ATGAGTTTCC CAACCCAGCT GCCCATGCTC TCCATGTTAC TTGTGTGGAG 
CTCATGGCCT TGGCAGTTTC AGGCAAAGAA GTTGGGAATG CCCTTCTAAA TGTTGTCCTA 
AAAAGTCAGC CTTTAGTGCC AAGAGAGAAC ATTACAGCAT GGATGAATGC AATTGGTTTG 
ATCATCACTG CCCTACCAGA GCCATATTGG ATTGTTCTTC ATGATCGAAT TGTGAGTGTC 
ATCAGCAGCC CCAGCTTGAC GTCTGAAACA GAGTGGGTTG GCTATCCATT CCGCCTCTTT 
GATTTCACTG CCTGTCATCA GTCCTACTCT GAGATGAGTT GTAGCTATAC GTTAGCTCTT 
GCACATGCTG TGTGGCACCA TTCTAGCATC GGACAACTTT CTCTCATTCC AAAGTTTCTT 
ACTGAAGTAC TTCTTCCTAT AGTGAAGACC GAATTCCAGT TGCTTTATGT ATACCATCTT 
GTTGGACCAT TTTTACAAAG ATTTCAGCAA GAGAGAACTC GTTGTATGAT AGAGATTGGT 
GTGGCGTTTT ATGACATGCT GCTGAATGTT GACCAGTGTA GCACCCATTT AAATTACATG 
GATCCCATCT GTGACTTCCT CTATCACATG AAGTATATGT TTACTGGTGA CAGCGTGAAA 
GAGCAAGTAG AGAAGATTAT CTGTAACTTA AAACCAGCTT TAAAACTTCG TCTTCGATTC 
ATCACACACA TTAGCAAGAT GGAGCCAGCT GCAGTGCCTC CACAAGCCAT GAACAGTGGG 
TCTCCAGCAC CTCAGTCTAA TCAGGTGCCC GTGTCTTTAC CAGTAACTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866990.1
CDS	82..3255
Translation	MERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQVPVSLPVTQ

Target ORF information:

RefSeq Version	XM_017011501.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011501.1

ORF Insert Sequence:

ATGGAGCGAT CTGAGACCGA GGAGAAGTTT GACGATGGGG GAACAAGCCA ACTCCTGTGG 
CAGCATCTCT CAAGTCAGCT CATTTTCTTT GTGCTTTTCC AGTTTGCAAG TTTTCCACAT 
ATGGTGCTTT CTCTTCATCA GAAGTTAGCA GGGCGAGGAC TGATTAAAGG CAGAGATCAT 
CTTATGTGGG TTCTCCTGCA ATTCATTTCT GGAAGTATTC AGAAAAATGC ACTAGCTGAT 
TTTCTCCCTG TGATGAAGCT CTTCGACTTG CTATACCCAG AAAAAGAATA TATCCCAGTT 
CCTGATATTA ACAAACCCCA GTCAACCCAT GCCTTTGCAA TGACCTGTAT TTGGATTCAT 
CTCAATAGAA AAGCTCAAAA TGACAACTCC AAGCTACAGA TTCCAATACC TCATTCCCTA 
AGACTTCACC ATGAGTTCCT GCAGCAGAGT CTAAGAAATA AAAGTTTACA GATGAATGAC 
TATAAGATTG CTCTATTGTG TAATGCATAC TCTACAAATT CAGAATGTTT TACATTACCC 
ATGGGAGCTC TGGTAGAAAC TATTTATGGA AATGGAATTA TGAGGATACC TCTCCCTGGA 
ACAAACTGTA TGGCTTCAGG ATCTATTACC CCCTTACCTA TGAACCTCCT GGATTCACTG 
ACAGTTCATG CCAAAATGAG CCTTATTCAC AGCATTGCAA CCAGGGTGAT AAAACTTGCT 
CATGCAAAGT CCAGTGTGGC CTTGGCTCCA GCCCTAGTGG AAACTTACAG TCGTTTATTG 
GTCTATATGG AAATAGAGTC TTTGGGCATC AAAGGATTTA TCAGTCAGCT TTTGCCAACT 
GTTTTCAAAT CACATGCATG GGGGATCTTA CACACACTCC TTGAGATGTT TAGCTACCGG 
ATGCATCATA TTCAGCCTCA TTACAGAGTT CAGCTCCTGA GTCATCTTCA TACTTTGGCT 
GCAGTTGCAC AAACAAACCA GAACCAGCTC CATCTTTGTG TCGAGAGCAC TGCTCTCAGG 
CTTATAACAG CATTAGGTAG CTCAGAGGTA CAACCGCAGT TTACACGCTT CCTTAGTGAT 
CCCAAAACAG TGCTCTCAGC AGAATCTGAA GAACTGAACC GAGCCTTGAT ATTGACCTTG 
GCTAGAGCAA CTCATGTAAC AGATTTTTTT ACAGGCTCTG ATTCAATTCA GGGAACTTGG 
TGTAAAGACA TACTTCAGAC CATCATGAGT TTCACTCCTC ATAATTGGGC TTCACACACC 
CTGAGCTGTT TTCCAGGCCC ACTACAGGCA TTCTTCAAAC AAAATAATGT GCCTCAGGAA 
AGCCGTTTTA ATCTGAAAAA AAATGTGGAG GAGGAGTATA GGAAGTGGAA GTCAATGAGC 
AACGAAAACG ACATTATTAC CCACTTCTCT ATGCAGGGCT CCCCTCCTCT CTTTCTTTGT 
CTTCTCTGGA AAATGCTCTT GGAAACAGAT CATATTAATC AGATTGGCTA TAGAGTATTA 
GAGAGAATTG GAGCCAGGGC CTTGGTAGCC CATGTGAGGA CATTTGCAGA TTTCCTGGTA 
TATGAGTTTT CTACATCAGC AGGGGGTCAG CAACTCAATA AATGCATTGA AATTCTTAAT 
GACATGGTAT GGAAGTATAA CATTGTTACA CTGGACAGAT TAATTCTCTG CCTGGCCATG 
CGTAGTCACG AAGGAAATGA AGCCCAGGTT TGTTATTTCA TAATTCAGTT GCTGTTACTC 
AAACCAAACG ATTTTAGAAA TCGAGTAAGT GACTTTGTGA AGGAAAATTC CCCAGAGCAC 
TGGTTACAGA ATGACTGGCA CACCAAGCAC ATGAATTATC ACAAGAAATA TCCAGAGAAG 
TTGTATTTTG AGGGCCTCGC GGAACAGGTG GATCCTCCTG TACAGATCCA GTCTCCCTAT 
CTGCCCATCT ATTTTGGGAA TGTGTGTCTT CGATTCCTTC CAGTATTTGA TATAGTAATC 
CACAGATTTT TAGAGTTGCT TCCGGTATCC AAATCACTGG AGACTCTACT GGATCATCTA 
GGAGGCTTAT ATAAATTTCA TGATCGTCCA GTGACTTATC TGTATAACAC TCTGCACTAT 
TATGAAATGC ACCTGAGAGA CCGCGCATTT CTCAAACGAA AACTCGTCCA TGCGATCATT 
GGCTCTCTGA AGGATAATCG ACCGCAGGGC TGGTGTCTAA GTGACACTTA CCTGAAATGC 
GCTATGAATG CACGAGAGGA AAATCCTTGG GTTCCAGATG ACACCTACTA TTGCAGATTG 
ATTGGCAGAC TAGTCGATAC GATGGCTGGC AAATCTCCTG GTCCCTTTCC AAACTGTGAC 
TGGAGATTCA ATGAGTTTCC CAACCCAGCT GCCCATGCTC TCCATGTTAC TTGTGTGGAG 
CTCATGGCCT TGGCAGTTTC AGGCAAAGAA GTTGGGAATG CCCTTCTAAA TGTTGTCCTA 
AAAAGTCAGC CTTTAGTGCC AAGAGAGAAC ATTACAGCAT GGATGAATGC AATTGGTTTG 
ATCATCACTG CCCTACCAGA GCCATATTGG ATTGTTCTTC ATGATCGAAT TGTGAGTGTC 
ATCAGCAGCC CCAGCTTGAC GTCTGAAACA GAGTGGGTTG GCTATCCATT CCGCCTCTTT 
GATTTCACTG CCTGTCATCA GTCCTACTCT GAGATGAGTT GTAGCTATAC GTTAGCTCTT 
GCACATGCTG TGTGGCACCA TTCTAGCATC GGACAACTTT CTCTCATTCC AAAGTTTCTT 
ACTGAAGTAC TTCTTCCTAT AGTGAAGACC GAATTCCAGT TGCTTTATGT ATACCATCTT 
GTTGGACCAT TTTTACAAAG ATTTCAGCAA GAGAGAACTC GTTGTATGAT AGAGATTGGT 
GTGGCGTTTT ATGACATGCT GCTGAATGTT GACCAGTGTA GCACCCATTT AAATTACATG 
GATCCCATCT GTGACTTCCT CTATCACATG AAGTATATGT TTACTGGTGA CAGCGTGAAA 
GAGCAAGTAG AGAAGATTAT CTGTAACTTA AAACCAGCTT TAAAACTTCG TCTTCGATTC 
ATCACACACA TTAGCAAGAT GGAGCCAGCT GCAGTGCCTC CACAAGCCAT GAACAGTGGG 
TCTCCAGCAC CTCAGTCTAA TCAGGTGCCC GTGTCTTTAC CAGTAACTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( NM_004830.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004821.2 Homo sapiens mediator complex subunit 23 (MED23), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04644
Clone ID Related Accession (Same CDS sequence)	NM_004830.4 , NM_004830.3
Accession Version	NM_004830.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121575.24, DB453121.1, AB033042.1 and AI249380.1. On Nov 22, 2018 this sequence version replaced NM_004830.3. Transcript Variant: This variant (1) encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.74491.1, SRR1660807.75901.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000368068.8/ ENSP00000357047.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG 
GAGGACCAGT TGGTGGATCT GGTTGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG 
TTTGACGATG GGGGAACAAG CCAACTCCTG TGGCAGCATC TCTCAAGTCA GCTCATTTTC 
TTTGTGCTTT TCCAGTTTGC AAGTTTTCCA CATATGGTGC TTTCTCTTCA TCAGAAGTTA 
GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCCT GCAATTCATT 
TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTCC CTGTGATGAA GCTCTTCGAC 
TTGCTATACC CAGAAAAAGA ATATATCCCA GTTCCTGATA TTAACAAACC CCAGTCAACC 
CATGCCTTTG CAATGACCTG TATTTGGATT CATCTCAATA GAAAAGCTCA AAATGACAAC 
TCCAAGCTAC AGATTCCAAT ACCTCATTCC CTAAGACTTC ACCATGAGTT CCTGCAGCAG 
AGTCTAAGAA ATAAAAGTTT ACAGATGAAT GACTATAAGA TTGCTCTATT GTGTAATGCA 
TACTCTACAA ATTCAGAATG TTTTACATTA CCCATGGGAG CTCTGGTAGA AACTATTTAT 
GGAAATGGAA TTATGAGGAT ACCTCTCCCT GGAACAAACT GTATGGCTTC AGGATCTATT 
ACCCCCTTAC CTATGAACCT CCTGGATTCA CTGACAGTTC ATGCCAAAAT GAGCCTTATT 
CACAGCATTG CAACCAGGGT GATAAAACTT GCTCATGCAA AGTCCAGTGT GGCCTTGGCT 
CCAGCCCTAG TGGAAACTTA CAGTCGTTTA TTGGTCTATA TGGAAATAGA GTCTTTGGGC 
ATCAAAGGAT TTATCAGTCA GCTTTTGCCA ACTGTTTTCA AATCACATGC ATGGGGGATC 
TTACACACAC TCCTTGAGAT GTTTAGCTAC CGGATGCATC ATATTCAGCC TCATTACAGA 
GTTCAGCTCC TGAGTCATCT TCATACTTTG GCTGCAGTTG CACAAACAAA CCAGAACCAG 
CTCCATCTTT GTGTCGAGAG CACTGCTCTC AGGCTTATAA CAGCATTAGG TAGCTCAGAG 
GTACAACCGC AGTTTACACG CTTCCTTAGT GATCCCAAAA CAGTGCTCTC AGCAGAATCT 
GAAGAACTGA ACCGAGCCTT GATATTGACC TTGGCTAGAG CAACTCATGT AACAGATTTT 
TTTACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG 
AGTTTCACTC CTCATAATTG GGCTTCACAC ACCCTGAGCT GTTTTCCAGG CCCACTACAG 
GCATTCTTCA AACAAAATAA TGTGCCTCAG GAAAGCCGTT TTAATCTGAA AAAAAATGTG 
GAGGAGGAGT ATAGGAAGTG GAAGTCAATG AGCAACGAAA ACGACATTAT TACCCACTTC 
TCTATGCAGG GCTCCCCTCC TCTCTTTCTT TGTCTTCTCT GGAAAATGCT CTTGGAAACA 
GATCATATTA ATCAGATTGG CTATAGAGTA TTAGAGAGAA TTGGAGCCAG GGCCTTGGTA 
GCCCATGTGA GGACATTTGC AGATTTCCTG GTATATGAGT TTTCTACATC AGCAGGGGGT 
CAGCAACTCA ATAAATGCAT TGAAATTCTT AATGACATGG TATGGAAGTA TAACATTGTT 
ACACTGGACA GATTAATTCT CTGCCTGGCC ATGCGTAGTC ACGAAGGAAA TGAAGCCCAG 
GTTTGTTATT TCATAATTCA GTTGCTGTTA CTCAAACCAA ACGATTTTAG AAATCGAGTA 
AGTGACTTTG TGAAGGAAAA TTCCCCAGAG CACTGGTTAC AGAATGACTG GCACACCAAG 
CACATGAATT ATCACAAGAA ATATCCAGAG AAGTTGTATT TTGAGGGCCT CGCGGAACAG 
GTGGATCCTC CTGTACAGAT CCAGTCTCCC TATCTGCCCA TCTATTTTGG GAATGTGTGT 
CTTCGATTCC TTCCAGTATT TGATATAGTA ATCCACAGAT TTTTAGAGTT GCTTCCGGTA 
TCCAAATCAC TGGAGACTCT ACTGGATCAT CTAGGAGGCT TATATAAATT TCATGATCGT 
CCAGTGACTT ATCTGTATAA CACTCTGCAC TATTATGAAA TGCACCTGAG AGACCGCGCA 
TTTCTCAAAC GAAAACTCGT CCATGCGATC ATTGGCTCTC TGAAGGATAA TCGACCGCAG 
GGCTGGTGTC TAAGTGACAC TTACCTGAAA TGCGCTATGA ATGCACGAGA GGAAAATCCT 
TGGGTTCCAG ATGACACCTA CTATTGCAGA TTGATTGGCA GACTAGTCGA TACGATGGCT 
GGCAAATCTC CTGGTCCCTT TCCAAACTGT GACTGGAGAT TCAATGAGTT TCCCAACCCA 
GCTGCCCATG CTCTCCATGT TACTTGTGTG GAGCTCATGG CCTTGGCAGT TTCAGGCAAA 
GAAGTTGGGA ATGCCCTTCT AAATGTTGTC CTAAAAAGTC AGCCTTTAGT GCCAAGAGAG 
AACATTACAG CATGGATGAA TGCAATTGGT TTGATCATCA CTGCCCTACC AGAGCCATAT 
TGGATTGTTC TTCATGATCG AATTGTGAGT GTCATCAGCA GCCCCAGCTT GACGTCTGAA 
ACAGAGTGGG TTGGCTATCC ATTCCGCCTC TTTGATTTCA CTGCCTGTCA TCAGTCCTAC 
TCTGAGATGA GTTGTAGCTA TACGTTAGCT CTTGCACATG CTGTGTGGCA CCATTCTAGC 
ATCGGACAAC TTTCTCTCAT TCCAAAGTTT CTTACTGAAG TACTTCTTCC TATAGTGAAG 
ACCGAATTCC AGTTGCTTTA TGTATACCAT CTTGTTGGAC CATTTTTACA AAGATTTCAG 
CAAGAGAGAA CTCGTTGTAT GATAGAGATT GGTGTGGCGT TTTATGACAT GCTGCTGAAT 
GTTGACCAGT GTAGCACCCA TTTAAATTAC ATGGATCCCA TCTGTGACTT CCTCTATCAC 
ATGAAGTATA TGTTTACTGG TGACAGCGTG AAAGAGCAAG TAGAGAAGAT TATCTGTAAC 
TTAAAACCAG CTTTAAAACT TCGTCTTCGA TTCATCACAC ACATTAGCAA GATGGAGCCA 
GCTGCAGTGC CTCCACAAGC CATGAACAGT GGGTCTCCAG CACCTCAGTC TAATCAGGTG 
CCCGTGTCTT TACCAGTAAC TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004821.2
CDS	194..4300
Translation	METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQVPVSLPVTQ

Target ORF information:

RefSeq Version	NM_004830.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004830.4

ORF Insert Sequence:

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG 
GAGGACCAGT TGGTGGATCT GGTTGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG 
TTTGACGATG GGGGAACAAG CCAACTCCTG TGGCAGCATC TCTCAAGTCA GCTCATTTTC 
TTTGTGCTTT TCCAGTTTGC AAGTTTTCCA CATATGGTGC TTTCTCTTCA TCAGAAGTTA 
GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCCT GCAATTCATT 
TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTCC CTGTGATGAA GCTCTTCGAC 
TTGCTATACC CAGAAAAAGA ATATATCCCA GTTCCTGATA TTAACAAACC CCAGTCAACC 
CATGCCTTTG CAATGACCTG TATTTGGATT CATCTCAATA GAAAAGCTCA AAATGACAAC 
TCCAAGCTAC AGATTCCAAT ACCTCATTCC CTAAGACTTC ACCATGAGTT CCTGCAGCAG 
AGTCTAAGAA ATAAAAGTTT ACAGATGAAT GACTATAAGA TTGCTCTATT GTGTAATGCA 
TACTCTACAA ATTCAGAATG TTTTACATTA CCCATGGGAG CTCTGGTAGA AACTATTTAT 
GGAAATGGAA TTATGAGGAT ACCTCTCCCT GGAACAAACT GTATGGCTTC AGGATCTATT 
ACCCCCTTAC CTATGAACCT CCTGGATTCA CTGACAGTTC ATGCCAAAAT GAGCCTTATT 
CACAGCATTG CAACCAGGGT GATAAAACTT GCTCATGCAA AGTCCAGTGT GGCCTTGGCT 
CCAGCCCTAG TGGAAACTTA CAGTCGTTTA TTGGTCTATA TGGAAATAGA GTCTTTGGGC 
ATCAAAGGAT TTATCAGTCA GCTTTTGCCA ACTGTTTTCA AATCACATGC ATGGGGGATC 
TTACACACAC TCCTTGAGAT GTTTAGCTAC CGGATGCATC ATATTCAGCC TCATTACAGA 
GTTCAGCTCC TGAGTCATCT TCATACTTTG GCTGCAGTTG CACAAACAAA CCAGAACCAG 
CTCCATCTTT GTGTCGAGAG CACTGCTCTC AGGCTTATAA CAGCATTAGG TAGCTCAGAG 
GTACAACCGC AGTTTACACG CTTCCTTAGT GATCCCAAAA CAGTGCTCTC AGCAGAATCT 
GAAGAACTGA ACCGAGCCTT GATATTGACC TTGGCTAGAG CAACTCATGT AACAGATTTT 
TTTACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG 
AGTTTCACTC CTCATAATTG GGCTTCACAC ACCCTGAGCT GTTTTCCAGG CCCACTACAG 
GCATTCTTCA AACAAAATAA TGTGCCTCAG GAAAGCCGTT TTAATCTGAA AAAAAATGTG 
GAGGAGGAGT ATAGGAAGTG GAAGTCAATG AGCAACGAAA ACGACATTAT TACCCACTTC 
TCTATGCAGG GCTCCCCTCC TCTCTTTCTT TGTCTTCTCT GGAAAATGCT CTTGGAAACA 
GATCATATTA ATCAGATTGG CTATAGAGTA TTAGAGAGAA TTGGAGCCAG GGCCTTGGTA 
GCCCATGTGA GGACATTTGC AGATTTCCTG GTATATGAGT TTTCTACATC AGCAGGGGGT 
CAGCAACTCA ATAAATGCAT TGAAATTCTT AATGACATGG TATGGAAGTA TAACATTGTT 
ACACTGGACA GATTAATTCT CTGCCTGGCC ATGCGTAGTC ACGAAGGAAA TGAAGCCCAG 
GTTTGTTATT TCATAATTCA GTTGCTGTTA CTCAAACCAA ACGATTTTAG AAATCGAGTA 
AGTGACTTTG TGAAGGAAAA TTCCCCAGAG CACTGGTTAC AGAATGACTG GCACACCAAG 
CACATGAATT ATCACAAGAA ATATCCAGAG AAGTTGTATT TTGAGGGCCT CGCGGAACAG 
GTGGATCCTC CTGTACAGAT CCAGTCTCCC TATCTGCCCA TCTATTTTGG GAATGTGTGT 
CTTCGATTCC TTCCAGTATT TGATATAGTA ATCCACAGAT TTTTAGAGTT GCTTCCGGTA 
TCCAAATCAC TGGAGACTCT ACTGGATCAT CTAGGAGGCT TATATAAATT TCATGATCGT 
CCAGTGACTT ATCTGTATAA CACTCTGCAC TATTATGAAA TGCACCTGAG AGACCGCGCA 
TTTCTCAAAC GAAAACTCGT CCATGCGATC ATTGGCTCTC TGAAGGATAA TCGACCGCAG 
GGCTGGTGTC TAAGTGACAC TTACCTGAAA TGCGCTATGA ATGCACGAGA GGAAAATCCT 
TGGGTTCCAG ATGACACCTA CTATTGCAGA TTGATTGGCA GACTAGTCGA TACGATGGCT 
GGCAAATCTC CTGGTCCCTT TCCAAACTGT GACTGGAGAT TCAATGAGTT TCCCAACCCA 
GCTGCCCATG CTCTCCATGT TACTTGTGTG GAGCTCATGG CCTTGGCAGT TTCAGGCAAA 
GAAGTTGGGA ATGCCCTTCT AAATGTTGTC CTAAAAAGTC AGCCTTTAGT GCCAAGAGAG 
AACATTACAG CATGGATGAA TGCAATTGGT TTGATCATCA CTGCCCTACC AGAGCCATAT 
TGGATTGTTC TTCATGATCG AATTGTGAGT GTCATCAGCA GCCCCAGCTT GACGTCTGAA 
ACAGAGTGGG TTGGCTATCC ATTCCGCCTC TTTGATTTCA CTGCCTGTCA TCAGTCCTAC 
TCTGAGATGA GTTGTAGCTA TACGTTAGCT CTTGCACATG CTGTGTGGCA CCATTCTAGC 
ATCGGACAAC TTTCTCTCAT TCCAAAGTTT CTTACTGAAG TACTTCTTCC TATAGTGAAG 
ACCGAATTCC AGTTGCTTTA TGTATACCAT CTTGTTGGAC CATTTTTACA AAGATTTCAG 
CAAGAGAGAA CTCGTTGTAT GATAGAGATT GGTGTGGCGT TTTATGACAT GCTGCTGAAT 
GTTGACCAGT GTAGCACCCA TTTAAATTAC ATGGATCCCA TCTGTGACTT CCTCTATCAC 
ATGAAGTATA TGTTTACTGG TGACAGCGTG AAAGAGCAAG TAGAGAAGAT TATCTGTAAC 
TTAAAACCAG CTTTAAAACT TCGTCTTCGA TTCATCACAC ACATTAGCAA GATGGAGCCA 
GCTGCAGTGC CTCCACAAGC CATGAACAGT GGGTCTCCAG CACCTCAGTC TAATCAGGTG 
CCCGTGTCTT TACCAGTAAC TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( NM_004830.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004821.2 Homo sapiens mediator complex subunit 23 (MED23), transcript variant 1, mRNA.

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CloneID	OHu04644
Clone ID Related Accession (Same CDS sequence)	NM_004830.4 , NM_004830.3
Accession Version	NM_004830.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-27
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB453121.1, AB033042.1 and AI249380.1. On Jul 14, 2012 this sequence version replaced NM_004830.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.74491.1, SRR1660807.75901.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG 
GAGGACCAGT TGGTGGATCT GGTTGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG 
TTTGACGATG GGGGAACAAG CCAACTCCTG TGGCAGCATC TCTCAAGTCA GCTCATTTTC 
TTTGTGCTTT TCCAGTTTGC AAGTTTTCCA CATATGGTGC TTTCTCTTCA TCAGAAGTTA 
GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCCT GCAATTCATT 
TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTCC CTGTGATGAA GCTCTTCGAC 
TTGCTATACC CAGAAAAAGA ATATATCCCA GTTCCTGATA TTAACAAACC CCAGTCAACC 
CATGCCTTTG CAATGACCTG TATTTGGATT CATCTCAATA GAAAAGCTCA AAATGACAAC 
TCCAAGCTAC AGATTCCAAT ACCTCATTCC CTAAGACTTC ACCATGAGTT CCTGCAGCAG 
AGTCTAAGAA ATAAAAGTTT ACAGATGAAT GACTATAAGA TTGCTCTATT GTGTAATGCA 
TACTCTACAA ATTCAGAATG TTTTACATTA CCCATGGGAG CTCTGGTAGA AACTATTTAT 
GGAAATGGAA TTATGAGGAT ACCTCTCCCT GGAACAAACT GTATGGCTTC AGGATCTATT 
ACCCCCTTAC CTATGAACCT CCTGGATTCA CTGACAGTTC ATGCCAAAAT GAGCCTTATT 
CACAGCATTG CAACCAGGGT GATAAAACTT GCTCATGCAA AGTCCAGTGT GGCCTTGGCT 
CCAGCCCTAG TGGAAACTTA CAGTCGTTTA TTGGTCTATA TGGAAATAGA GTCTTTGGGC 
ATCAAAGGAT TTATCAGTCA GCTTTTGCCA ACTGTTTTCA AATCACATGC ATGGGGGATC 
TTACACACAC TCCTTGAGAT GTTTAGCTAC CGGATGCATC ATATTCAGCC TCATTACAGA 
GTTCAGCTCC TGAGTCATCT TCATACTTTG GCTGCAGTTG CACAAACAAA CCAGAACCAG 
CTCCATCTTT GTGTCGAGAG CACTGCTCTC AGGCTTATAA CAGCATTAGG TAGCTCAGAG 
GTACAACCGC AGTTTACACG CTTCCTTAGT GATCCCAAAA CAGTGCTCTC AGCAGAATCT 
GAAGAACTGA ACCGAGCCTT GATATTGACC TTGGCTAGAG CAACTCATGT AACAGATTTT 
TTTACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG 
AGTTTCACTC CTCATAATTG GGCTTCACAC ACCCTGAGCT GTTTTCCAGG CCCACTACAG 
GCATTCTTCA AACAAAATAA TGTGCCTCAG GAAAGCCGTT TTAATCTGAA AAAAAATGTG 
GAGGAGGAGT ATAGGAAGTG GAAGTCAATG AGCAACGAAA ACGACATTAT TACCCACTTC 
TCTATGCAGG GCTCCCCTCC TCTCTTTCTT TGTCTTCTCT GGAAAATGCT CTTGGAAACA 
GATCATATTA ATCAGATTGG CTATAGAGTA TTAGAGAGAA TTGGAGCCAG GGCCTTGGTA 
GCCCATGTGA GGACATTTGC AGATTTCCTG GTATATGAGT TTTCTACATC AGCAGGGGGT 
CAGCAACTCA ATAAATGCAT TGAAATTCTT AATGACATGG TATGGAAGTA TAACATTGTT 
ACACTGGACA GATTAATTCT CTGCCTGGCC ATGCGTAGTC ACGAAGGAAA TGAAGCCCAG 
GTTTGTTATT TCATAATTCA GTTGCTGTTA CTCAAACCAA ACGATTTTAG AAATCGAGTA 
AGTGACTTTG TGAAGGAAAA TTCCCCAGAG CACTGGTTAC AGAATGACTG GCACACCAAG 
CACATGAATT ATCACAAGAA ATATCCAGAG AAGTTGTATT TTGAGGGCCT CGCGGAACAG 
GTGGATCCTC CTGTACAGAT CCAGTCTCCC TATCTGCCCA TCTATTTTGG GAATGTGTGT 
CTTCGATTCC TTCCAGTATT TGATATAGTA ATCCACAGAT TTTTAGAGTT GCTTCCGGTA 
TCCAAATCAC TGGAGACTCT ACTGGATCAT CTAGGAGGCT TATATAAATT TCATGATCGT 
CCAGTGACTT ATCTGTATAA CACTCTGCAC TATTATGAAA TGCACCTGAG AGACCGCGCA 
TTTCTCAAAC GAAAACTCGT CCATGCGATC ATTGGCTCTC TGAAGGATAA TCGACCGCAG 
GGCTGGTGTC TAAGTGACAC TTACCTGAAA TGCGCTATGA ATGCACGAGA GGAAAATCCT 
TGGGTTCCAG ATGACACCTA CTATTGCAGA TTGATTGGCA GACTAGTCGA TACGATGGCT 
GGCAAATCTC CTGGTCCCTT TCCAAACTGT GACTGGAGAT TCAATGAGTT TCCCAACCCA 
GCTGCCCATG CTCTCCATGT TACTTGTGTG GAGCTCATGG CCTTGGCAGT TTCAGGCAAA 
GAAGTTGGGA ATGCCCTTCT AAATGTTGTC CTAAAAAGTC AGCCTTTAGT GCCAAGAGAG 
AACATTACAG CATGGATGAA TGCAATTGGT TTGATCATCA CTGCCCTACC AGAGCCATAT 
TGGATTGTTC TTCATGATCG AATTGTGAGT GTCATCAGCA GCCCCAGCTT GACGTCTGAA 
ACAGAGTGGG TTGGCTATCC ATTCCGCCTC TTTGATTTCA CTGCCTGTCA TCAGTCCTAC 
TCTGAGATGA GTTGTAGCTA TACGTTAGCT CTTGCACATG CTGTGTGGCA CCATTCTAGC 
ATCGGACAAC TTTCTCTCAT TCCAAAGTTT CTTACTGAAG TACTTCTTCC TATAGTGAAG 
ACCGAATTCC AGTTGCTTTA TGTATACCAT CTTGTTGGAC CATTTTTACA AAGATTTCAG 
CAAGAGAGAA CTCGTTGTAT GATAGAGATT GGTGTGGCGT TTTATGACAT GCTGCTGAAT 
GTTGACCAGT GTAGCACCCA TTTAAATTAC ATGGATCCCA TCTGTGACTT CCTCTATCAC 
ATGAAGTATA TGTTTACTGG TGACAGCGTG AAAGAGCAAG TAGAGAAGAT TATCTGTAAC 
TTAAAACCAG CTTTAAAACT TCGTCTTCGA TTCATCACAC ACATTAGCAA GATGGAGCCA 
GCTGCAGTGC CTCCACAAGC CATGAACAGT GGGTCTCCAG CACCTCAGTC TAATCAGGTG 
CCCGTGTCTT TACCAGTAAC TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004821.2
CDS	191..4297
Translation	METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQVPVSLPVTQ

Target ORF information:

RefSeq Version	NM_004830.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004830.3

ORF Insert Sequence:

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG 
GAGGACCAGT TGGTGGATCT GGTTGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG 
TTTGACGATG GGGGAACAAG CCAACTCCTG TGGCAGCATC TCTCAAGTCA GCTCATTTTC 
TTTGTGCTTT TCCAGTTTGC AAGTTTTCCA CATATGGTGC TTTCTCTTCA TCAGAAGTTA 
GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCCT GCAATTCATT 
TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTCC CTGTGATGAA GCTCTTCGAC 
TTGCTATACC CAGAAAAAGA ATATATCCCA GTTCCTGATA TTAACAAACC CCAGTCAACC 
CATGCCTTTG CAATGACCTG TATTTGGATT CATCTCAATA GAAAAGCTCA AAATGACAAC 
TCCAAGCTAC AGATTCCAAT ACCTCATTCC CTAAGACTTC ACCATGAGTT CCTGCAGCAG 
AGTCTAAGAA ATAAAAGTTT ACAGATGAAT GACTATAAGA TTGCTCTATT GTGTAATGCA 
TACTCTACAA ATTCAGAATG TTTTACATTA CCCATGGGAG CTCTGGTAGA AACTATTTAT 
GGAAATGGAA TTATGAGGAT ACCTCTCCCT GGAACAAACT GTATGGCTTC AGGATCTATT 
ACCCCCTTAC CTATGAACCT CCTGGATTCA CTGACAGTTC ATGCCAAAAT GAGCCTTATT 
CACAGCATTG CAACCAGGGT GATAAAACTT GCTCATGCAA AGTCCAGTGT GGCCTTGGCT 
CCAGCCCTAG TGGAAACTTA CAGTCGTTTA TTGGTCTATA TGGAAATAGA GTCTTTGGGC 
ATCAAAGGAT TTATCAGTCA GCTTTTGCCA ACTGTTTTCA AATCACATGC ATGGGGGATC 
TTACACACAC TCCTTGAGAT GTTTAGCTAC CGGATGCATC ATATTCAGCC TCATTACAGA 
GTTCAGCTCC TGAGTCATCT TCATACTTTG GCTGCAGTTG CACAAACAAA CCAGAACCAG 
CTCCATCTTT GTGTCGAGAG CACTGCTCTC AGGCTTATAA CAGCATTAGG TAGCTCAGAG 
GTACAACCGC AGTTTACACG CTTCCTTAGT GATCCCAAAA CAGTGCTCTC AGCAGAATCT 
GAAGAACTGA ACCGAGCCTT GATATTGACC TTGGCTAGAG CAACTCATGT AACAGATTTT 
TTTACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG 
AGTTTCACTC CTCATAATTG GGCTTCACAC ACCCTGAGCT GTTTTCCAGG CCCACTACAG 
GCATTCTTCA AACAAAATAA TGTGCCTCAG GAAAGCCGTT TTAATCTGAA AAAAAATGTG 
GAGGAGGAGT ATAGGAAGTG GAAGTCAATG AGCAACGAAA ACGACATTAT TACCCACTTC 
TCTATGCAGG GCTCCCCTCC TCTCTTTCTT TGTCTTCTCT GGAAAATGCT CTTGGAAACA 
GATCATATTA ATCAGATTGG CTATAGAGTA TTAGAGAGAA TTGGAGCCAG GGCCTTGGTA 
GCCCATGTGA GGACATTTGC AGATTTCCTG GTATATGAGT TTTCTACATC AGCAGGGGGT 
CAGCAACTCA ATAAATGCAT TGAAATTCTT AATGACATGG TATGGAAGTA TAACATTGTT 
ACACTGGACA GATTAATTCT CTGCCTGGCC ATGCGTAGTC ACGAAGGAAA TGAAGCCCAG 
GTTTGTTATT TCATAATTCA GTTGCTGTTA CTCAAACCAA ACGATTTTAG AAATCGAGTA 
AGTGACTTTG TGAAGGAAAA TTCCCCAGAG CACTGGTTAC AGAATGACTG GCACACCAAG 
CACATGAATT ATCACAAGAA ATATCCAGAG AAGTTGTATT TTGAGGGCCT CGCGGAACAG 
GTGGATCCTC CTGTACAGAT CCAGTCTCCC TATCTGCCCA TCTATTTTGG GAATGTGTGT 
CTTCGATTCC TTCCAGTATT TGATATAGTA ATCCACAGAT TTTTAGAGTT GCTTCCGGTA 
TCCAAATCAC TGGAGACTCT ACTGGATCAT CTAGGAGGCT TATATAAATT TCATGATCGT 
CCAGTGACTT ATCTGTATAA CACTCTGCAC TATTATGAAA TGCACCTGAG AGACCGCGCA 
TTTCTCAAAC GAAAACTCGT CCATGCGATC ATTGGCTCTC TGAAGGATAA TCGACCGCAG 
GGCTGGTGTC TAAGTGACAC TTACCTGAAA TGCGCTATGA ATGCACGAGA GGAAAATCCT 
TGGGTTCCAG ATGACACCTA CTATTGCAGA TTGATTGGCA GACTAGTCGA TACGATGGCT 
GGCAAATCTC CTGGTCCCTT TCCAAACTGT GACTGGAGAT TCAATGAGTT TCCCAACCCA 
GCTGCCCATG CTCTCCATGT TACTTGTGTG GAGCTCATGG CCTTGGCAGT TTCAGGCAAA 
GAAGTTGGGA ATGCCCTTCT AAATGTTGTC CTAAAAAGTC AGCCTTTAGT GCCAAGAGAG 
AACATTACAG CATGGATGAA TGCAATTGGT TTGATCATCA CTGCCCTACC AGAGCCATAT 
TGGATTGTTC TTCATGATCG AATTGTGAGT GTCATCAGCA GCCCCAGCTT GACGTCTGAA 
ACAGAGTGGG TTGGCTATCC ATTCCGCCTC TTTGATTTCA CTGCCTGTCA TCAGTCCTAC 
TCTGAGATGA GTTGTAGCTA TACGTTAGCT CTTGCACATG CTGTGTGGCA CCATTCTAGC 
ATCGGACAAC TTTCTCTCAT TCCAAAGTTT CTTACTGAAG TACTTCTTCC TATAGTGAAG 
ACCGAATTCC AGTTGCTTTA TGTATACCAT CTTGTTGGAC CATTTTTACA AAGATTTCAG 
CAAGAGAGAA CTCGTTGTAT GATAGAGATT GGTGTGGCGT TTTATGACAT GCTGCTGAAT 
GTTGACCAGT GTAGCACCCA TTTAAATTAC ATGGATCCCA TCTGTGACTT CCTCTATCAC 
ATGAAGTATA TGTTTACTGG TGACAGCGTG AAAGAGCAAG TAGAGAAGAT TATCTGTAAC 
TTAAAACCAG CTTTAAAACT TCGTCTTCGA TTCATCACAC ACATTAGCAA GATGGAGCCA 
GCTGCAGTGC CTCCACAAGC CATGAACAGT GGGTCTCCAG CACCTCAGTC TAATCAGGTG 
CCCGTGTCTT TACCAGTAAC TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( NM_001376518.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363447.1 Homo sapiens mediator complex subunit 23 (MED23), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu109117
Clone ID Related Accession (Same CDS sequence)	NM_001376518.1
Accession Version	NM_001376518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121575.24. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.134439.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTATGG ATACTCCTGA AGATGAGAAA ACAAAACTAA TTAGCTGTTT GGGGGCCTTC 
AGACAGTTTT GGGGTGGTCT TTCTCAGGAG TCTCATGAAC AGTGTATCCA GTGGATTGTT 
AAGTTTATTC ATGGTCAGCA TAGTCCTAAA AGAATTTCTT TTCTTTATGA CTGCTTAGCA 
ATGGCAGTTG AGACTGGTCT CCTTCCACCC AGGCTGGTTT GTGAATCCCT GATAAACTCT 
GACACTCTTG AGTGGGAAAG AACACAGCTT TGGGCCTTAA CATTTAAACT GGTTCGGAAA 
ATAATTGGGG GAGTGGATTA CAAGGGTGTT CGAGATCTCT TAAAAGTGAT TTTGGAGAAG 
ATTTTGACAA TTCCTAATAC AGTGAGCTCT GCTGTTGTAC AGCAGCTTCT GGCAGCAAGA 
GAGGTTATAG CATATATCTT GGAAAGAAAT GCCTGCTTAT TACCAGCCTA TTTTGCAGTC 
ACTGAGATCA GGAAACTGTA TCCTGAAGGC AAACTTCCAC ACTGGTTACT TGGAAACCTA 
GTATCAGACT TTGTGGATAC CTTCAGGCCC ACAGCAAGGA TAAACTCCAT TTGTGGTCGC 
TGTAGTCTTC TGCCAGTTGT AAATAATTCG GGTGCCATTT GTAATTCATG GAAACTGGAT 
CCTGCTACTC TTCGTTTTCC TTTGAAAGGC CTTTTGCCAT ATGATAAGGA TCTGTTTGAA 
CCACAGACTG CTTTGTTGAG ATATGTATTG GAGCAGCCTT ATTCCAGGGA TATGGTCTGC 
AATATGCTAG GTTTAAATAA GCAGACCTTG AACATTGCTC AGCACAAGCA GCGCTGCCCT 
GTGCTGGAGG ACCAGTTGGT GGATCTGGTT GTTTATGCCA TGGAGCGATC TGAGACCGAG 
GAGAAGTTTG ACGATGGGGG AACAAGCCAA CTCCTGTGGC AGCATCTCTC AAGTCAGCTC 
ATTTTCTTTG TGCTTTTCCA GTTTGCAAGT TTTCCACATA TGGTGCTTTC TCTTCATCAG 
AAGTTAGCAG GGCGAGGACT GATTAAAGGC AGAGATCATC TTATGTGGGT TCTCCTGCAA 
TTCATTTCTG GAAGTATTCA GAAAAATGCA CTAGCTGATT TTCTCCCTGT GATGAAGCTC 
TTCGACTTGC TATACCCAGA AAAAGAATAT ATCCCAGTTC CTGATATTAA CAAACCCCAG 
TCAACCCATG CCTTTGCAAT GACCTGTATT TGGATTCATC TCAATAGAAA AGCTCAAAAT 
GACAACTCCA AGCTACAGAT TCCAATACCT CATTCCCTAA GACTTCACCA TGAGTTCCTG 
CAGCAGAGTC TAAGAAATAA AAGTTTACAG ATGAATGACT ATAAGATTGC TCTATTGTGT 
AATGCATACT CTACAAATTC AGAATGTTTT ACATTACCCA TGGGAGCTCT GGTAGAAACT 
ATTTATGGAA ATGGAATTAT GAGGATACCT CTCCCTGGAA CAAACTGTAT GGCTTCAGGA 
TCTATTACCC CCTTACCTAT GAACCTCCTG GATTCACTGA CAGTTCATGC CAAAATGAGC 
CTTATTCACA GCATTGCAAC CAGGGTGATA AAACTTGCTC ATGCAAAGTC CAGTGTGGCC 
TTGGCTCCAG CCCTAGTGGA AACTTACAGT CGTTTATTGG TCTATATGGA AATAGAGTCT 
TTGGGCATCA AAGGATTTAT CAGTCAGCTT TTGCCAACTG TTTTCAAATC ACATGCATGG 
GGGATCTTAC ACACACTCCT TGAGATGTTT AGCTACCGGA TGCATCATAT TCAGCCTCAT 
TACAGAGTTC AGCTCCTGAG TCATCTTCAT ACTTTGGCTG CAGTTGCACA AACAAACCAG 
AACCAGCTCC ATCTTTGTGT CGAGAGCACT GCTCTCAGGC TTATAACAGC ATTAGGTAGC 
TCAGAGGTAC AACCGCAGTT TACACGCTTC CTTAGTGATC CCAAAACAGT GCTCTCAGCA 
GAATCTGAAG AACTGAACCG AGCCTTGATA TTGACCTTGG CTAGAGCAAC TCATGTAACA 
GATTTTTTTA CAGGCTCTGA TTCAATTCAG GGAACTTGGT GTAAAGACAT ACTTCAGACC 
ATCATGAGTT TCACTCCTCA TAATTGGGCT TCACACACCC TGAGCTGTTT TCCAGGCCCA 
CTACAGGCAT TCTTCAAACA AAATAATGTG CCTCAGGAAA GCCGTTTTAA TCTGAAAAAA 
AATGTGGAGG AGGAGTATAG GAAGTGGAAG TCAATGAGCA ACGAAAACGA CATTATTACC 
CACTTCTCTA TGCAGGGCTC CCCTCCTCTC TTTCTTTGTC TTCTCTGGAA AATGCTCTTG 
GAAACAGATC ATATTAATCA GATTGGCTAT AGAGTATTAG AGAGAATTGG AGCCAGGGCC 
TTGGTAGCCC ATGTGAGGAC ATTTGCAGAT TTCCTGGTAT ATGAGTTTTC TACATCAGCA 
GGGGGTCAGC AACTCAATAA ATGCATTGAA ATTCTTAATG ACATGGTATG GAAGTATAAC 
ATTGTTACAC TGGACAGATT AATTCTCTGC CTGGCCATGC GTAGTCACGA AGGAAATGAA 
GCCCAGGTTT GTTATTTCAT AATTCAGTTG CTGTTACTCA AACCAAACGA TTTTAGAAAT 
CGAGTAAGTG ACTTTGTGAA GGAAAATTCC CCAGAGCACT GGTTACAGAA TGACTGGCAC 
ACCAAGCACA TGAATTATCA CAAGAAATAT CCAGAGAAGT TGTATTTTGA GGGCCTCGCG 
GAACAGGTGG ATCCTCCTGT ACAGATCCAG TCTCCCTATC TGCCCATCTA TTTTGGGAAT 
GTGTGTCTTC GATTCCTTCC AGTATTTGAT ATAGTAATCC ACAGATTTTT AGAGTTGCTT 
CCGGTATCCA AATCACTGGA GACTCTACTG GATCATCTAG GAGGCTTATA TAAATTTCAT 
GATCGTCCAG TGACTTATCT GTATAACACT CTGCACTATT ATGAAATGCA CCTGAGAGAC 
CGCGCATTTC TCAAACGAAA ACTCGTCCAT GCGATCATTG GCTCTCTGAA GGATAATCGA 
CCGCAGGGCT GGTGTCTAAG TGACACTTAC CTGAAATGCG CTATGAATGC ACGAGAGGAA 
AATCCTTGGG TTCCAGATGA CACCTACTAT TGCAGATTGA TTGGCAGACT AGTCGATACG 
ATGGCTGGCA AATCTCCTGG TCCCTTTCCA AACTGTGACT GGAGATTCAA TGAGTTTCCC 
AACCCAGCTG CCCATGCTCT CCATGTTACT TGTGTGGAGC TCATGGCCTT GGCAGTTTCA 
GGCAAAGAAG TTGGGAATGC CCTTCTAAAT GTTGTCCTAA AAAGTCAGCC TTTAGTGCCA 
AGAGAGAACA TTACAGCATG GATGAATGCA ATTGGTTTGA TCATCACTGC CCTACCAGAG 
CCATATTGGA TTGTTCTTCA TGATCGAATT GTGAGTGTCA TCAGCAGCCC CAGCTTGACG 
TCTGAAACAG AGTGGGTTGG CTATCCATTC CGCCTCTTTG ATTTCACTGC CTGTCATCAG 
TCCTACTCTG AGATGAGTTG TAGCTATACG TTAGCTCTTG CACATGCTGT GTGGCACCAT 
TCTAGCATCG GACAACTTTC TCTCATTCCA AAGTTTCTTA CTGAAGTACT TCTTCCTATA 
GTGAAGACCG AATTCCAGTT GCTTTATGTA TACCATCTTG TTGGACCATT TTTACAAAGA 
TTTCAGCAAG AGAGAACTCG TTGTATGATA GAGATTGGTG TGGCGTTTTA TGACATGCTG 
CTGAATGTTG ACCAGTGTAG CACCCATTTA AATTACATGG ATCCCATCTG TGACTTCCTC 
TATCACATGA AGTATATGTT TACTGGTGAC AGCGTGAAAG AGCAAGTAGA GAAGATTATC 
TGTAACTTAA AACCAGCTTT AAAACTTCGT CTTCGATTCA TCACACACAT TAGCAAGATG 
GAGCCAGCTG CAGTGCCTCC ACAAGCCATG AACAGTGGGT CTCCAGCACC TCAGTCTAAT 
CAGGTGCCCG TGTCTTTACC AGTAACTCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363447.1
CDS	85..4137
Translation	MFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQTLNIAQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQVPVSLPVTQ

Target ORF information:

RefSeq Version	NM_001376518.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376518.1

ORF Insert Sequence:

ATGTTTATGG ATACTCCTGA AGATGAGAAA ACAAAACTAA TTAGCTGTTT GGGGGCCTTC 
AGACAGTTTT GGGGTGGTCT TTCTCAGGAG TCTCATGAAC AGTGTATCCA GTGGATTGTT 
AAGTTTATTC ATGGTCAGCA TAGTCCTAAA AGAATTTCTT TTCTTTATGA CTGCTTAGCA 
ATGGCAGTTG AGACTGGTCT CCTTCCACCC AGGCTGGTTT GTGAATCCCT GATAAACTCT 
GACACTCTTG AGTGGGAAAG AACACAGCTT TGGGCCTTAA CATTTAAACT GGTTCGGAAA 
ATAATTGGGG GAGTGGATTA CAAGGGTGTT CGAGATCTCT TAAAAGTGAT TTTGGAGAAG 
ATTTTGACAA TTCCTAATAC AGTGAGCTCT GCTGTTGTAC AGCAGCTTCT GGCAGCAAGA 
GAGGTTATAG CATATATCTT GGAAAGAAAT GCCTGCTTAT TACCAGCCTA TTTTGCAGTC 
ACTGAGATCA GGAAACTGTA TCCTGAAGGC AAACTTCCAC ACTGGTTACT TGGAAACCTA 
GTATCAGACT TTGTGGATAC CTTCAGGCCC ACAGCAAGGA TAAACTCCAT TTGTGGTCGC 
TGTAGTCTTC TGCCAGTTGT AAATAATTCG GGTGCCATTT GTAATTCATG GAAACTGGAT 
CCTGCTACTC TTCGTTTTCC TTTGAAAGGC CTTTTGCCAT ATGATAAGGA TCTGTTTGAA 
CCACAGACTG CTTTGTTGAG ATATGTATTG GAGCAGCCTT ATTCCAGGGA TATGGTCTGC 
AATATGCTAG GTTTAAATAA GCAGACCTTG AACATTGCTC AGCACAAGCA GCGCTGCCCT 
GTGCTGGAGG ACCAGTTGGT GGATCTGGTT GTTTATGCCA TGGAGCGATC TGAGACCGAG 
GAGAAGTTTG ACGATGGGGG AACAAGCCAA CTCCTGTGGC AGCATCTCTC AAGTCAGCTC 
ATTTTCTTTG TGCTTTTCCA GTTTGCAAGT TTTCCACATA TGGTGCTTTC TCTTCATCAG 
AAGTTAGCAG GGCGAGGACT GATTAAAGGC AGAGATCATC TTATGTGGGT TCTCCTGCAA 
TTCATTTCTG GAAGTATTCA GAAAAATGCA CTAGCTGATT TTCTCCCTGT GATGAAGCTC 
TTCGACTTGC TATACCCAGA AAAAGAATAT ATCCCAGTTC CTGATATTAA CAAACCCCAG 
TCAACCCATG CCTTTGCAAT GACCTGTATT TGGATTCATC TCAATAGAAA AGCTCAAAAT 
GACAACTCCA AGCTACAGAT TCCAATACCT CATTCCCTAA GACTTCACCA TGAGTTCCTG 
CAGCAGAGTC TAAGAAATAA AAGTTTACAG ATGAATGACT ATAAGATTGC TCTATTGTGT 
AATGCATACT CTACAAATTC AGAATGTTTT ACATTACCCA TGGGAGCTCT GGTAGAAACT 
ATTTATGGAA ATGGAATTAT GAGGATACCT CTCCCTGGAA CAAACTGTAT GGCTTCAGGA 
TCTATTACCC CCTTACCTAT GAACCTCCTG GATTCACTGA CAGTTCATGC CAAAATGAGC 
CTTATTCACA GCATTGCAAC CAGGGTGATA AAACTTGCTC ATGCAAAGTC CAGTGTGGCC 
TTGGCTCCAG CCCTAGTGGA AACTTACAGT CGTTTATTGG TCTATATGGA AATAGAGTCT 
TTGGGCATCA AAGGATTTAT CAGTCAGCTT TTGCCAACTG TTTTCAAATC ACATGCATGG 
GGGATCTTAC ACACACTCCT TGAGATGTTT AGCTACCGGA TGCATCATAT TCAGCCTCAT 
TACAGAGTTC AGCTCCTGAG TCATCTTCAT ACTTTGGCTG CAGTTGCACA AACAAACCAG 
AACCAGCTCC ATCTTTGTGT CGAGAGCACT GCTCTCAGGC TTATAACAGC ATTAGGTAGC 
TCAGAGGTAC AACCGCAGTT TACACGCTTC CTTAGTGATC CCAAAACAGT GCTCTCAGCA 
GAATCTGAAG AACTGAACCG AGCCTTGATA TTGACCTTGG CTAGAGCAAC TCATGTAACA 
GATTTTTTTA CAGGCTCTGA TTCAATTCAG GGAACTTGGT GTAAAGACAT ACTTCAGACC 
ATCATGAGTT TCACTCCTCA TAATTGGGCT TCACACACCC TGAGCTGTTT TCCAGGCCCA 
CTACAGGCAT TCTTCAAACA AAATAATGTG CCTCAGGAAA GCCGTTTTAA TCTGAAAAAA 
AATGTGGAGG AGGAGTATAG GAAGTGGAAG TCAATGAGCA ACGAAAACGA CATTATTACC 
CACTTCTCTA TGCAGGGCTC CCCTCCTCTC TTTCTTTGTC TTCTCTGGAA AATGCTCTTG 
GAAACAGATC ATATTAATCA GATTGGCTAT AGAGTATTAG AGAGAATTGG AGCCAGGGCC 
TTGGTAGCCC ATGTGAGGAC ATTTGCAGAT TTCCTGGTAT ATGAGTTTTC TACATCAGCA 
GGGGGTCAGC AACTCAATAA ATGCATTGAA ATTCTTAATG ACATGGTATG GAAGTATAAC 
ATTGTTACAC TGGACAGATT AATTCTCTGC CTGGCCATGC GTAGTCACGA AGGAAATGAA 
GCCCAGGTTT GTTATTTCAT AATTCAGTTG CTGTTACTCA AACCAAACGA TTTTAGAAAT 
CGAGTAAGTG ACTTTGTGAA GGAAAATTCC CCAGAGCACT GGTTACAGAA TGACTGGCAC 
ACCAAGCACA TGAATTATCA CAAGAAATAT CCAGAGAAGT TGTATTTTGA GGGCCTCGCG 
GAACAGGTGG ATCCTCCTGT ACAGATCCAG TCTCCCTATC TGCCCATCTA TTTTGGGAAT 
GTGTGTCTTC GATTCCTTCC AGTATTTGAT ATAGTAATCC ACAGATTTTT AGAGTTGCTT 
CCGGTATCCA AATCACTGGA GACTCTACTG GATCATCTAG GAGGCTTATA TAAATTTCAT 
GATCGTCCAG TGACTTATCT GTATAACACT CTGCACTATT ATGAAATGCA CCTGAGAGAC 
CGCGCATTTC TCAAACGAAA ACTCGTCCAT GCGATCATTG GCTCTCTGAA GGATAATCGA 
CCGCAGGGCT GGTGTCTAAG TGACACTTAC CTGAAATGCG CTATGAATGC ACGAGAGGAA 
AATCCTTGGG TTCCAGATGA CACCTACTAT TGCAGATTGA TTGGCAGACT AGTCGATACG 
ATGGCTGGCA AATCTCCTGG TCCCTTTCCA AACTGTGACT GGAGATTCAA TGAGTTTCCC 
AACCCAGCTG CCCATGCTCT CCATGTTACT TGTGTGGAGC TCATGGCCTT GGCAGTTTCA 
GGCAAAGAAG TTGGGAATGC CCTTCTAAAT GTTGTCCTAA AAAGTCAGCC TTTAGTGCCA 
AGAGAGAACA TTACAGCATG GATGAATGCA ATTGGTTTGA TCATCACTGC CCTACCAGAG 
CCATATTGGA TTGTTCTTCA TGATCGAATT GTGAGTGTCA TCAGCAGCCC CAGCTTGACG 
TCTGAAACAG AGTGGGTTGG CTATCCATTC CGCCTCTTTG ATTTCACTGC CTGTCATCAG 
TCCTACTCTG AGATGAGTTG TAGCTATACG TTAGCTCTTG CACATGCTGT GTGGCACCAT 
TCTAGCATCG GACAACTTTC TCTCATTCCA AAGTTTCTTA CTGAAGTACT TCTTCCTATA 
GTGAAGACCG AATTCCAGTT GCTTTATGTA TACCATCTTG TTGGACCATT TTTACAAAGA 
TTTCAGCAAG AGAGAACTCG TTGTATGATA GAGATTGGTG TGGCGTTTTA TGACATGCTG 
CTGAATGTTG ACCAGTGTAG CACCCATTTA AATTACATGG ATCCCATCTG TGACTTCCTC 
TATCACATGA AGTATATGTT TACTGGTGAC AGCGTGAAAG AGCAAGTAGA GAAGATTATC 
TGTAACTTAA AACCAGCTTT AAAACTTCGT CTTCGATTCA TCACACACAT TAGCAAGATG 
GAGCCAGCTG CAGTGCCTCC ACAAGCCATG AACAGTGGGT CTCCAGCACC TCAGTCTAAT 
CAGGTGCCCG TGTCTTTACC AGTAACTCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( NM_001376522.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363451.1 Homo sapiens mediator complex subunit 23 (MED23), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu109121
Clone ID Related Accession (Same CDS sequence)	NM_001376522.1
Accession Version	NM_001376522.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform j
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121575.24. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.84374.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG 
GAGGACCAGT TGGTGGATCT GGTTGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG 
TTTGACGATG GGGGAACAAG CCAACTCCTG TGGCAGCATC TCTCAAGTCA GCTCATTTTC 
TTTGTGCTTT TCCAGTTTGC AAGTTTTCCA CATATGGTGC TTTCTCTTCA TCAGAAGTTA 
GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCCT GCAATTCATT 
TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTCC CTGTGATGAA GCTCTTCGAC 
TTGCTATACC CAGAAAAAGA ATATATCCCA GTTCCTGATA TTAACAAACC CCAGTCAACC 
CATGCCTTTG CAATGACCTG TATTTGGATT CATCTCAATA GAAAAGCTCA AAATGACAAC 
TCCAAGCTAC AGATTCCAAT ACCTCATTCC CTAAGACTTC ACCATGAGTT CCTGCAGCAG 
AGTCTAAGAA ATAAAAGTTT ACAGATGAAT GACTATAAGA TTGCTCTATT GTGTAATGCA 
TACTCTACAA ATTCAGAATG TTTTACATTA CCCATGGGAG CTCTGGTAGA AACTATTTAT 
GGAAATGGAA TTATGAGGAT ACCTCTCCCT GGAACAAACT GTATGGCTTC AGGATCTATT 
ACCCCCTTAC CTATGAACCT CCTGGATTCA CTGACAGTTC ATGCCAAAAT GAGCCTTATT 
CACAGCATTG CAACCAGGGT GATAAAACTT GCTCATGCAA AGTCCAGTGT GGCCTTGGCT 
CCAGCCCTAG TGGAAACTTA CAGTCGTTTA TTGGTCTATA TGGAAATAGA GTCTTTGGGC 
ATCAAAGGAT TTATCAGTCA GCTTTTGCCA ACTGTTTTCA AATCACATGC ATGGGGGATC 
TTACACACAC TCCTTGAGAT GTTTAGCTAC CGGATGCATC ATATTCAGCC TCATTACAGA 
GTTCAGCTCC TGAGTCATCT TCATACTTTG GCTGCAGTTG CACAAACAAA CCAGAACCAG 
CTCCATCTTT GTGTCGAGAG CACTGCTCTC AGGCTTATAA CAGCATTAGG TAGCTCAGAG 
GTACAACCGC AGTTTACACG CTTCCTTAGT GATCCCAAAA CAGTGCTCTC AGCAGAATCT 
GAAGAACTGA ACCGAGCCTT GATATTGACC TTGGCTAGAG CAACTCATGT AACAGATTTT 
TTTACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG 
AGTTTCACTC CTCATAATTG GGCTTCACAC ACCCTGAGCT GTTTTCCAGG CCCACTACAG 
GCATTCTTCA AACAAAATAA TGTGCCTCAG GAAAGCCGTT TTAATCTGAA AAAAAATGTG 
GAGGAGGAGT ATAGGAAGTG GAAGTCAATG AGCAACGAAA ACGACATTAT TACCCACTTC 
TCTATGCAGG GCTCCCCTCC TCTCTTTCTT TGTCTTCTCT GGAAAATGCT CTTGGAAACA 
GATCATATTA ATCAGATTGG CTATAGAGTA TTAGAGAGAA TTGGAGCCAG GGCCTTGGTA 
GCCCATGTGA GGACATTTGC AGATTTCCTG GTATATGAGT TTTCTACATC AGCAGGGGGT 
CAGCAACTCA ATAAATGCAT TGAAATTCTT AATGACATGG TATGGAAGTA TAACATTGTT 
ACACTGGACA GATTAATTCT CTGCCTGAAA TATCCAGAGA AGTTGTATTT TGAGGGCCTC 
GCGGAACAGG TGGATCCTCC TGTACAGATC CAGTCTCCCT ATCTGCCCAT CTATTTTGGG 
AATGTGTGTC TTCGATTCCT TCCAGTATTT GATATAGTAA TCCACAGATT TTTAGAGTTG 
CTTCCGGTAT CCAAATCACT GGAGACTCTA CTGGATCATC TAGGAGGCTT ATATAAATTT 
CATGATCGTC CAGTGACTTA TCTGTATAAC ACTCTGCACT ATTATGAAAT GCACCTGAGA 
GACCGCGCAT TTCTCAAACG AAAACTCGTC CATGCGATCA TTGGCTCTCT GAAGGATAAT 
CGACCGCAGG GCTGGTGTCT AAGTGACACT TACCTGAAAT GCGCTATGAA TGCACGAGAG 
GAAAATCCTT GGGTTCCAGA TGACACCTAC TATTGCAGAT TGATTGGCAG ACTAGTCGAT 
ACGATGGCTG GCAAATCTCC TGGTCCCTTT CCAAACTGTG ACTGGAGATT CAATGAGTTT 
CCCAACCCAG CTGCCCATGC TCTCCATGTT ACTTGTGTGG AGCTCATGGC CTTGGCAGTT 
TCAGGCAAAG AAGTTGGGAA TGCCCTTCTA AATGTTGTCC TAAAAAGTCA GCCTTTAGTG 
CCAAGAGAGA ACATTACAGC ATGGATGAAT GCAATTGGTT TGATCATCAC TGCCCTACCA 
GAGCCATATT GGATTGTTCT TCATGATCGA ATTGTGAGTG TCATCAGCAG CCCCAGCTTG 
ACGTCTGAAA CAGAGTGGGT TGGCTATCCA TTCCGCCTCT TTGATTTCAC TGCCTGTCAT 
CAGTCCTACT CTGAGATGAG TTGTAGCTAT ACGTTAGCTC TTGCACATGC TGTGTGGCAC 
CATTCTAGCA TCGGACAACT TTCTCTCATT CCAAAGTTTC TTACTGAAGT ACTTCTTCCT 
ATAGTGAAGA CCGAATTCCA GTTGCTTTAT GTATACCATC TTGTTGGACC ATTTTTACAA 
AGATTTCAGC AAGAGAGAAC TCGTTGTATG ATAGAGATTG GTGTGGCGTT TTATGACATG 
CTGCTGAATG TTGACCAGTG TAGCACCCAT TTAAATTACA TGGATCCCAT CTGTGACTTC 
CTCTATCACA TGAAGTATAT GTTTACTGGT GACAGCGTGA AAGAGCAAGT AGAGAAGATT 
ATCTGTAACT TAAAACCAGC TTTAAAACTT CGTCTTCGAT TCATCACACA CATTAGCAAG 
ATGGAGCCAG CTGCAGTGCC TCCACAAGCC ATGAACAGTG GGTCTCCAGC ACCTCAGTCT 
AATCAGGTGC CCGTGTCTTT ACCAGTAACT CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363451.1
CDS	194..4129
Translation	METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQVPVSLPVTQ

Target ORF information:

RefSeq Version	NM_001376522.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376522.1

ORF Insert Sequence:

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG 
GAGGACCAGT TGGTGGATCT GGTTGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG 
TTTGACGATG GGGGAACAAG CCAACTCCTG TGGCAGCATC TCTCAAGTCA GCTCATTTTC 
TTTGTGCTTT TCCAGTTTGC AAGTTTTCCA CATATGGTGC TTTCTCTTCA TCAGAAGTTA 
GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCCT GCAATTCATT 
TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTCC CTGTGATGAA GCTCTTCGAC 
TTGCTATACC CAGAAAAAGA ATATATCCCA GTTCCTGATA TTAACAAACC CCAGTCAACC 
CATGCCTTTG CAATGACCTG TATTTGGATT CATCTCAATA GAAAAGCTCA AAATGACAAC 
TCCAAGCTAC AGATTCCAAT ACCTCATTCC CTAAGACTTC ACCATGAGTT CCTGCAGCAG 
AGTCTAAGAA ATAAAAGTTT ACAGATGAAT GACTATAAGA TTGCTCTATT GTGTAATGCA 
TACTCTACAA ATTCAGAATG TTTTACATTA CCCATGGGAG CTCTGGTAGA AACTATTTAT 
GGAAATGGAA TTATGAGGAT ACCTCTCCCT GGAACAAACT GTATGGCTTC AGGATCTATT 
ACCCCCTTAC CTATGAACCT CCTGGATTCA CTGACAGTTC ATGCCAAAAT GAGCCTTATT 
CACAGCATTG CAACCAGGGT GATAAAACTT GCTCATGCAA AGTCCAGTGT GGCCTTGGCT 
CCAGCCCTAG TGGAAACTTA CAGTCGTTTA TTGGTCTATA TGGAAATAGA GTCTTTGGGC 
ATCAAAGGAT TTATCAGTCA GCTTTTGCCA ACTGTTTTCA AATCACATGC ATGGGGGATC 
TTACACACAC TCCTTGAGAT GTTTAGCTAC CGGATGCATC ATATTCAGCC TCATTACAGA 
GTTCAGCTCC TGAGTCATCT TCATACTTTG GCTGCAGTTG CACAAACAAA CCAGAACCAG 
CTCCATCTTT GTGTCGAGAG CACTGCTCTC AGGCTTATAA CAGCATTAGG TAGCTCAGAG 
GTACAACCGC AGTTTACACG CTTCCTTAGT GATCCCAAAA CAGTGCTCTC AGCAGAATCT 
GAAGAACTGA ACCGAGCCTT GATATTGACC TTGGCTAGAG CAACTCATGT AACAGATTTT 
TTTACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG 
AGTTTCACTC CTCATAATTG GGCTTCACAC ACCCTGAGCT GTTTTCCAGG CCCACTACAG 
GCATTCTTCA AACAAAATAA TGTGCCTCAG GAAAGCCGTT TTAATCTGAA AAAAAATGTG 
GAGGAGGAGT ATAGGAAGTG GAAGTCAATG AGCAACGAAA ACGACATTAT TACCCACTTC 
TCTATGCAGG GCTCCCCTCC TCTCTTTCTT TGTCTTCTCT GGAAAATGCT CTTGGAAACA 
GATCATATTA ATCAGATTGG CTATAGAGTA TTAGAGAGAA TTGGAGCCAG GGCCTTGGTA 
GCCCATGTGA GGACATTTGC AGATTTCCTG GTATATGAGT TTTCTACATC AGCAGGGGGT 
CAGCAACTCA ATAAATGCAT TGAAATTCTT AATGACATGG TATGGAAGTA TAACATTGTT 
ACACTGGACA GATTAATTCT CTGCCTGAAA TATCCAGAGA AGTTGTATTT TGAGGGCCTC 
GCGGAACAGG TGGATCCTCC TGTACAGATC CAGTCTCCCT ATCTGCCCAT CTATTTTGGG 
AATGTGTGTC TTCGATTCCT TCCAGTATTT GATATAGTAA TCCACAGATT TTTAGAGTTG 
CTTCCGGTAT CCAAATCACT GGAGACTCTA CTGGATCATC TAGGAGGCTT ATATAAATTT 
CATGATCGTC CAGTGACTTA TCTGTATAAC ACTCTGCACT ATTATGAAAT GCACCTGAGA 
GACCGCGCAT TTCTCAAACG AAAACTCGTC CATGCGATCA TTGGCTCTCT GAAGGATAAT 
CGACCGCAGG GCTGGTGTCT AAGTGACACT TACCTGAAAT GCGCTATGAA TGCACGAGAG 
GAAAATCCTT GGGTTCCAGA TGACACCTAC TATTGCAGAT TGATTGGCAG ACTAGTCGAT 
ACGATGGCTG GCAAATCTCC TGGTCCCTTT CCAAACTGTG ACTGGAGATT CAATGAGTTT 
CCCAACCCAG CTGCCCATGC TCTCCATGTT ACTTGTGTGG AGCTCATGGC CTTGGCAGTT 
TCAGGCAAAG AAGTTGGGAA TGCCCTTCTA AATGTTGTCC TAAAAAGTCA GCCTTTAGTG 
CCAAGAGAGA ACATTACAGC ATGGATGAAT GCAATTGGTT TGATCATCAC TGCCCTACCA 
GAGCCATATT GGATTGTTCT TCATGATCGA ATTGTGAGTG TCATCAGCAG CCCCAGCTTG 
ACGTCTGAAA CAGAGTGGGT TGGCTATCCA TTCCGCCTCT TTGATTTCAC TGCCTGTCAT 
CAGTCCTACT CTGAGATGAG TTGTAGCTAT ACGTTAGCTC TTGCACATGC TGTGTGGCAC 
CATTCTAGCA TCGGACAACT TTCTCTCATT CCAAAGTTTC TTACTGAAGT ACTTCTTCCT 
ATAGTGAAGA CCGAATTCCA GTTGCTTTAT GTATACCATC TTGTTGGACC ATTTTTACAA 
AGATTTCAGC AAGAGAGAAC TCGTTGTATG ATAGAGATTG GTGTGGCGTT TTATGACATG 
CTGCTGAATG TTGACCAGTG TAGCACCCAT TTAAATTACA TGGATCCCAT CTGTGACTTC 
CTCTATCACA TGAAGTATAT GTTTACTGGT GACAGCGTGA AAGAGCAAGT AGAGAAGATT 
ATCTGTAACT TAAAACCAGC TTTAAAACTT CGTCTTCGAT TCATCACACA CATTAGCAAG 
ATGGAGCCAG CTGCAGTGCC TCCACAAGCC ATGAACAGTG GGTCTCCAGC ACCTCAGTCT 
AATCAGGTGC CCGTGTCTTT ACCAGTAACT CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( NM_001376521.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363450.1 Homo sapiens mediator complex subunit 23 (MED23), transcript variant 9, mRNA.

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CloneID	OHu109126
Clone ID Related Accession (Same CDS sequence)	NM_001376521.1
Accession Version	NM_001376521.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform i
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121575.24. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.114481.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG 
GAGGACCAGT TGGTGGATCT GGTTGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG 
TTTGACGATG GGGGAACAAG CCAACTCCTG TGGCAGCATC TCTCAAGTCA GCTCATTTTC 
TTTGTGCTTT TCCAGTTTGC AAGTTTTCCA CATATGGTGC TTTCTCTTCA TCAGAAGTTA 
GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCCT GCAATTCATT 
TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTCC CTGTGATGAA GCTCTTCGAC 
TTGCTATACC CAGAAAAAGA ATATATCCCA GTTCCTGATA TTAACAAACC CCAGTCAACC 
CATGCCTTTG CAATGACCTG TATTTGGATT CATCTCAATA GAAAAGCTCA AAATGACAAC 
TCCAAGCTAC AGATTCCAAT ACCTCATTCC CTAAGACTTC ACCATGAGTT CCTGCAGCAG 
AGTCTAAGAA ATAAAAGTTT ACAGATGAAT GACTATAAGA TTGCTCTATT GTGTAATGCA 
TACTCTACAA ATTCAGAATG TTTTACATTA CCCATGGGAG CTCTGGTAGA AACTATTTAT 
GGAAATGGAA TTATGAGGAT ACCTCTCCCT GGAACAAACT GTATGGCTTC AGGATCTATT 
ACCCCCTTAC CTATGAACCT CCTGGATTCA CTGACAGTTC ATGCCAAAAT GAGCCTTATT 
CACAGCATTG CAACCAGGGT GATAAAACTT GCTCATGCAA AGTCCAGTGT GGCCTTGGCT 
CCAGCCCTAG TGGAAACTTA CAGTCGTTTA TTGGTCTATA TGGAAATAGA GTCTTTGGGC 
ATCAAAGGAT TTATCAGTCA GCTTTTGCCA ACTGTTTTCA AATCACATGC ATGGGGGATC 
TTACACACAC TCCTTGAGAT GTTTAGCTAC CGGATGCATC ATATTCAGCC TCATTACAGA 
GTTCAGCTCC TGAGTCATCT TCATACTTTG GCTGCAGTTG CACAAACAAA CCAGAACCAG 
CTCCATCTTT GTGTCGAGAG CACTGCTCTC AGGCTTATAA CAGCATTAGG TAGCTCAGAG 
GTACAACCGC AGTTTACACG CTTCCTTAGT GATCCCAAAA CAGTGCTCTC AGCAGAATCT 
GAAGAACTGA ACCGAGCCTT GATATTGACC TTGGCTAGAG CAACTCATGT AACAGATTTT 
TTTACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG 
AGTTTCACTC CTCATAATTG GGCTTCACAC ACCCTGAGCT GTTTTCCAGG CCCACTACAG 
GCATTCTTCA AACAAAATAA TGTGCCTCAG GAAAGCCGTT TTAATCTGAA AAAAAATGTG 
GAGGAGGAGT ATAGGAAGTG GAAGTCAATG AGCAACGAAA ACGACATTAT TACCCACTTC 
TCTATGCAGG GCTCCCCTCC TCTCTTTCTT TGTCTTCTCT GGAAAATGCT CTTGGAAACA 
GATCATATTA ATCAGATTGG CTATAGAGTA TTAGAGAGAA TTGGAGCCAG GGCCTTGGTA 
GCCCATGTGA GGACATTTGC AGATTTCCTG GTATATGAGT TTTCTACATC AGCAGGGGGT 
CAGCAACTCA ATAAATGCAT TGAAATTCTT AATGACATGG TATGGAAGTA TAACATTGTT 
ACACTGGACA GATTAATTCT CTGCCTGGCC ATGCGTAGTC ACGAAGGAAA TGAAGCCCAG 
AAATATCCAG AGAAGTTGTA TTTTGAGGGC CTCGCGGAAC AGGTGGATCC TCCTGTACAG 
ATCCAGTCTC CCTATCTGCC CATCTATTTT GGGAATGTGT GTCTTCGATT CCTTCCAGTA 
TTTGATATAG TAATCCACAG ATTTTTAGAG TTGCTTCCGG TATCCAAATC ACTGGAGACT 
CTACTGGATC ATCTAGGAGG CTTATATAAA TTTCATGATC GTCCAGTGAC TTATCTGTAT 
AACACTCTGC ACTATTATGA AATGCACCTG AGAGACCGCG CATTTCTCAA ACGAAAACTC 
GTCCATGCGA TCATTGGCTC TCTGAAGGAT AATCGACCGC AGGGCTGGTG TCTAAGTGAC 
ACTTACCTGA AATGCGCTAT GAATGCACGA GAGGAAAATC CTTGGGTTCC AGATGACACC 
TACTATTGCA GATTGATTGG CAGACTAGTC GATACGATGG CTGGCAAATC TCCTGGTCCC 
TTTCCAAACT GTGACTGGAG ATTCAATGAG TTTCCCAACC CAGCTGCCCA TGCTCTCCAT 
GTTACTTGTG TGGAGCTCAT GGCCTTGGCA GTTTCAGGCA AAGAAGTTGG GAATGCCCTT 
CTAAATGTTG TCCTAAAAAG TCAGCCTTTA GTGCCAAGAG AGAACATTAC AGCATGGATG 
AATGCAATTG GTTTGATCAT CACTGCCCTA CCAGAGCCAT ATTGGATTGT TCTTCATGAT 
CGAATTGTGA GTGTCATCAG CAGCCCCAGC TTGACGTCTG AAACAGAGTG GGTTGGCTAT 
CCATTCCGCC TCTTTGATTT CACTGCCTGT CATCAGTCCT ACTCTGAGAT GAGTTGTAGC 
TATACGTTAG CTCTTGCACA TGCTGTGTGG CACCATTCTA GCATCGGACA ACTTTCTCTC 
ATTCCAAAGT TTCTTACTGA AGTACTTCTT CCTATAGTGA AGACCGAATT CCAGTTGCTT 
TATGTATACC ATCTTGTTGG ACCATTTTTA CAAAGATTTC AGCAAGAGAG AACTCGTTGT 
ATGATAGAGA TTGGTGTGGC GTTTTATGAC ATGCTGCTGA ATGTTGACCA GTGTAGCACC 
CATTTAAATT ACATGGATCC CATCTGTGAC TTCCTCTATC ACATGAAGTA TATGTTTACT 
GGTGACAGCG TGAAAGAGCA AGTAGAGAAG ATTATCTGTA ACTTAAAACC AGCTTTAAAA 
CTTCGTCTTC GATTCATCAC ACACATTAGC AAGATGGAGC CAGCTGCAGT GCCTCCACAA 
GCCATGAACA GTGGGTCTCC AGCACCTCAG TCTAATCAGG TTGACACTCT CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363450.1
CDS	194..4150
Translation	METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQVDTLT

Target ORF information:

RefSeq Version	NM_001376521.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376521.1

ORF Insert Sequence:

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG 
GAGGACCAGT TGGTGGATCT GGTTGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG 
TTTGACGATG GGGGAACAAG CCAACTCCTG TGGCAGCATC TCTCAAGTCA GCTCATTTTC 
TTTGTGCTTT TCCAGTTTGC AAGTTTTCCA CATATGGTGC TTTCTCTTCA TCAGAAGTTA 
GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCCT GCAATTCATT 
TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTCC CTGTGATGAA GCTCTTCGAC 
TTGCTATACC CAGAAAAAGA ATATATCCCA GTTCCTGATA TTAACAAACC CCAGTCAACC 
CATGCCTTTG CAATGACCTG TATTTGGATT CATCTCAATA GAAAAGCTCA AAATGACAAC 
TCCAAGCTAC AGATTCCAAT ACCTCATTCC CTAAGACTTC ACCATGAGTT CCTGCAGCAG 
AGTCTAAGAA ATAAAAGTTT ACAGATGAAT GACTATAAGA TTGCTCTATT GTGTAATGCA 
TACTCTACAA ATTCAGAATG TTTTACATTA CCCATGGGAG CTCTGGTAGA AACTATTTAT 
GGAAATGGAA TTATGAGGAT ACCTCTCCCT GGAACAAACT GTATGGCTTC AGGATCTATT 
ACCCCCTTAC CTATGAACCT CCTGGATTCA CTGACAGTTC ATGCCAAAAT GAGCCTTATT 
CACAGCATTG CAACCAGGGT GATAAAACTT GCTCATGCAA AGTCCAGTGT GGCCTTGGCT 
CCAGCCCTAG TGGAAACTTA CAGTCGTTTA TTGGTCTATA TGGAAATAGA GTCTTTGGGC 
ATCAAAGGAT TTATCAGTCA GCTTTTGCCA ACTGTTTTCA AATCACATGC ATGGGGGATC 
TTACACACAC TCCTTGAGAT GTTTAGCTAC CGGATGCATC ATATTCAGCC TCATTACAGA 
GTTCAGCTCC TGAGTCATCT TCATACTTTG GCTGCAGTTG CACAAACAAA CCAGAACCAG 
CTCCATCTTT GTGTCGAGAG CACTGCTCTC AGGCTTATAA CAGCATTAGG TAGCTCAGAG 
GTACAACCGC AGTTTACACG CTTCCTTAGT GATCCCAAAA CAGTGCTCTC AGCAGAATCT 
GAAGAACTGA ACCGAGCCTT GATATTGACC TTGGCTAGAG CAACTCATGT AACAGATTTT 
TTTACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG 
AGTTTCACTC CTCATAATTG GGCTTCACAC ACCCTGAGCT GTTTTCCAGG CCCACTACAG 
GCATTCTTCA AACAAAATAA TGTGCCTCAG GAAAGCCGTT TTAATCTGAA AAAAAATGTG 
GAGGAGGAGT ATAGGAAGTG GAAGTCAATG AGCAACGAAA ACGACATTAT TACCCACTTC 
TCTATGCAGG GCTCCCCTCC TCTCTTTCTT TGTCTTCTCT GGAAAATGCT CTTGGAAACA 
GATCATATTA ATCAGATTGG CTATAGAGTA TTAGAGAGAA TTGGAGCCAG GGCCTTGGTA 
GCCCATGTGA GGACATTTGC AGATTTCCTG GTATATGAGT TTTCTACATC AGCAGGGGGT 
CAGCAACTCA ATAAATGCAT TGAAATTCTT AATGACATGG TATGGAAGTA TAACATTGTT 
ACACTGGACA GATTAATTCT CTGCCTGGCC ATGCGTAGTC ACGAAGGAAA TGAAGCCCAG 
AAATATCCAG AGAAGTTGTA TTTTGAGGGC CTCGCGGAAC AGGTGGATCC TCCTGTACAG 
ATCCAGTCTC CCTATCTGCC CATCTATTTT GGGAATGTGT GTCTTCGATT CCTTCCAGTA 
TTTGATATAG TAATCCACAG ATTTTTAGAG TTGCTTCCGG TATCCAAATC ACTGGAGACT 
CTACTGGATC ATCTAGGAGG CTTATATAAA TTTCATGATC GTCCAGTGAC TTATCTGTAT 
AACACTCTGC ACTATTATGA AATGCACCTG AGAGACCGCG CATTTCTCAA ACGAAAACTC 
GTCCATGCGA TCATTGGCTC TCTGAAGGAT AATCGACCGC AGGGCTGGTG TCTAAGTGAC 
ACTTACCTGA AATGCGCTAT GAATGCACGA GAGGAAAATC CTTGGGTTCC AGATGACACC 
TACTATTGCA GATTGATTGG CAGACTAGTC GATACGATGG CTGGCAAATC TCCTGGTCCC 
TTTCCAAACT GTGACTGGAG ATTCAATGAG TTTCCCAACC CAGCTGCCCA TGCTCTCCAT 
GTTACTTGTG TGGAGCTCAT GGCCTTGGCA GTTTCAGGCA AAGAAGTTGG GAATGCCCTT 
CTAAATGTTG TCCTAAAAAG TCAGCCTTTA GTGCCAAGAG AGAACATTAC AGCATGGATG 
AATGCAATTG GTTTGATCAT CACTGCCCTA CCAGAGCCAT ATTGGATTGT TCTTCATGAT 
CGAATTGTGA GTGTCATCAG CAGCCCCAGC TTGACGTCTG AAACAGAGTG GGTTGGCTAT 
CCATTCCGCC TCTTTGATTT CACTGCCTGT CATCAGTCCT ACTCTGAGAT GAGTTGTAGC 
TATACGTTAG CTCTTGCACA TGCTGTGTGG CACCATTCTA GCATCGGACA ACTTTCTCTC 
ATTCCAAAGT TTCTTACTGA AGTACTTCTT CCTATAGTGA AGACCGAATT CCAGTTGCTT 
TATGTATACC ATCTTGTTGG ACCATTTTTA CAAAGATTTC AGCAAGAGAG AACTCGTTGT 
ATGATAGAGA TTGGTGTGGC GTTTTATGAC ATGCTGCTGA ATGTTGACCA GTGTAGCACC 
CATTTAAATT ACATGGATCC CATCTGTGAC TTCCTCTATC ACATGAAGTA TATGTTTACT 
GGTGACAGCG TGAAAGAGCA AGTAGAGAAG ATTATCTGTA ACTTAAAACC AGCTTTAAAA 
CTTCGTCTTC GATTCATCAC ACACATTAGC AAGATGGAGC CAGCTGCAGT GCCTCCACAA 
GCCATGAACA GTGGGTCTCC AGCACCTCAG TCTAATCAGG TTGACACTCT CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( NM_001376520.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363449.1 Homo sapiens mediator complex subunit 23 (MED23), transcript variant 8, mRNA.

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CloneID	OHu109129
Clone ID Related Accession (Same CDS sequence)	NM_001376520.1
Accession Version	NM_001376520.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121575.24. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.187359.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGACCT TGAACATTGC TCAGTTTGCA 
AGTTTTCCAC ATATGGTGCT TTCTCTTCAT CAGAAGTTAG CAGGGCGAGG ACTGATTAAA 
GGCAGAGATC ATCTTATGTG GGTTCTCCTG CAATTCATTT CTGGAAGTAT TCAGAAAAAT 
GCACTAGCTG ATTTTCTCCC TGTGATGAAG CTCTTCGACT TGCTATACCC AGAAAAAGAA 
TATATCCCAG TTCCTGATAT TAACAAACCC CAGTCAACCC ATGCCTTTGC AATGACCTGT 
ATTTGGATTC ATCTCAATAG AAAAGCTCAA AATGACAACT CCAAGCTACA GATTCCAATA 
CCTCATTCCC TAAGACTTCA CCATGAGTTC CTGCAGCAGA GTCTAAGAAA TAAAAGTTTA 
CAGATGAATG ACTATAAGAT TGCTCTATTG TGTAATGCAT ACTCTACAAA TTCAGAATGT 
TTTACATTAC CCATGGGAGC TCTGGTAGAA ACTATTTATG GAAATGGAAT TATGAGGATA 
CCTCTCCCTG GAACAAACTG TATGGCTTCA GGATCTATTA CCCCCTTACC TATGAACCTC 
CTGGATTCAC TGACAGTTCA TGCCAAAATG AGCCTTATTC ACAGCATTGC AACCAGGGTG 
ATAAAACTTG CTCATGCAAA GTCCAGTGTG GCCTTGGCTC CAGCCCTAGT GGAAACTTAC 
AGTCGTTTAT TGGTCTATAT GGAAATAGAG TCTTTGGGCA TCAAAGGATT TATCAGTCAG 
CTTTTGCCAA CTGTTTTCAA ATCACATGCA TGGGGGATCT TACACACACT CCTTGAGATG 
TTTAGCTACC GGATGCATCA TATTCAGCCT CATTACAGAG TTCAGCTCCT GAGTCATCTT 
CATACTTTGG CTGCAGTTGC ACAAACAAAC CAGAACCAGC TCCATCTTTG TGTCGAGAGC 
ACTGCTCTCA GGCTTATAAC AGCATTAGGT AGCTCAGAGG TACAACCGCA GTTTACACGC 
TTCCTTAGTG ATCCCAAAAC AGTGCTCTCA GCAGAATCTG AAGAACTGAA CCGAGCCTTG 
ATATTGACCT TGGCTAGAGC AACTCATGTA ACAGATTTTT TTACAGGCTC TGATTCAATT 
CAGGGAACTT GGTGTAAAGA CATACTTCAG ACCATCATGA GTTTCACTCC TCATAATTGG 
GCTTCACACA CCCTGAGCTG TTTTCCAGGC CCACTACAGG CATTCTTCAA ACAAAATAAT 
GTGCCTCAGG AAAGCCGTTT TAATCTGAAA AAAAATGTGG AGGAGGAGTA TAGGAAGTGG 
AAGTCAATGA GCAACGAAAA CGACATTATT ACCCACTTCT CTATGCAGGG CTCCCCTCCT 
CTCTTTCTTT GTCTTCTCTG GAAAATGCTC TTGGAAACAG ATCATATTAA TCAGATTGGC 
TATAGAGTAT TAGAGAGAAT TGGAGCCAGG GCCTTGGTAG CCCATGTGAG GACATTTGCA 
GATTTCCTGG TATATGAGTT TTCTACATCA GCAGGGGGTC AGCAACTCAA TAAATGCATT 
GAAATTCTTA ATGACATGGT ATGGAAGTAT AACATTGTTA CACTGGACAG ATTAATTCTC 
TGCCTGGCCA TGCGTAGTCA CGAAGGAAAT GAAGCCCAGG TTTGTTATTT CATAATTCAG 
TTGCTGTTAC TCAAACCAAA CGATTTTAGA AATCGAGTAA GTGACTTTGT GAAGGAAAAT 
TCCCCAGAGC ACTGGTTACA GAATGACTGG CACACCAAGC ACATGAATTA TCACAAGAAA 
TATCCAGAGA AGTTGTATTT TGAGGGCCTC GCGGAACAGG TGGATCCTCC TGTACAGATC 
CAGTCTCCCT ATCTGCCCAT CTATTTTGGG AATGTGTGTC TTCGATTCCT TCCAGTATTT 
GATATAGTAA TCCACAGATT TTTAGAGTTG CTTCCGGTAT CCAAATCACT GGAGACTCTA 
CTGGATCATC TAGGAGGCTT ATATAAATTT CATGATCGTC CAGTGACTTA TCTGTATAAC 
ACTCTGCACT ATTATGAAAT GCACCTGAGA GACCGCGCAT TTCTCAAACG AAAACTCGTC 
CATGCGATCA TTGGCTCTCT GAAGGATAAT CGACCGCAGG GCTGGTGTCT AAGTGACACT 
TACCTGAAAT GCGCTATGAA TGCACGAGAG GAAAATCCTT GGGTTCCAGA TGACACCTAC 
TATTGCAGAT TGATTGGCAG ACTAGTCGAT ACGATGGCTG GCAAATCTCC TGGTCCCTTT 
CCAAACTGTG ACTGGAGATT CAATGAGTTT CCCAACCCAG CTGCCCATGC TCTCCATGTT 
ACTTGTGTGG AGCTCATGGC CTTGGCAGTT TCAGGCAAAG AAGTTGGGAA TGCCCTTCTA 
AATGTTGTCC TAAAAAGTCA GCCTTTAGTG CCAAGAGAGA ACATTACAGC ATGGATGAAT 
GCAATTGGTT TGATCATCAC TGCCCTACCA GAGCCATATT GGATTGTTCT TCATGATCGA 
ATTGTGAGTG TCATCAGCAG CCCCAGCTTG ACGTCTGAAA CAGAGTGGGT TGGCTATCCA 
TTCCGCCTCT TTGATTTCAC TGCCTGTCAT CAGTCCTACT CTGAGATGAG TTGTAGCTAT 
ACGTTAGCTC TTGCACATGC TGTGTGGCAC CATTCTAGCA TCGGACAACT TTCTCTCATT 
CCAAAGTTTC TTACTGAAGT ACTTCTTCCT ATAGTGAAGA CCGAATTCCA GTTGCTTTAT 
GTATACCATC TTGTTGGACC ATTTTTACAA AGATTTCAGC AAGAGAGAAC TCGTTGTATG 
ATAGAGATTG GTGTGGCGTT TTATGACATG CTGCTGAATG TTGACCAGTG TAGCACCCAT 
TTAAATTACA TGGATCCCAT CTGTGACTTC CTCTATCACA TGAAGTATAT GTTTACTGGT 
GACAGCGTGA AAGAGCAAGT AGAGAAGATT ATCTGTAACT TAAAACCAGC TTTAAAACTT 
CGTCTTCGAT TCATCACACA CATTAGCAAG ATGGAGCCAG CTGCAGTGCC TCCACAAGCC 
ATGAACAGTG GGTCTCCAGC ACCTCAGTCT AATCAGGTGC CCGTGTCTTT ACCAGTAACT 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363449.1
CDS	194..4159
Translation	METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQTLNIAQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQVPVSLPVTQ

Target ORF information:

RefSeq Version	NM_001376520.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376520.1

ORF Insert Sequence:

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGACCT TGAACATTGC TCAGTTTGCA 
AGTTTTCCAC ATATGGTGCT TTCTCTTCAT CAGAAGTTAG CAGGGCGAGG ACTGATTAAA 
GGCAGAGATC ATCTTATGTG GGTTCTCCTG CAATTCATTT CTGGAAGTAT TCAGAAAAAT 
GCACTAGCTG ATTTTCTCCC TGTGATGAAG CTCTTCGACT TGCTATACCC AGAAAAAGAA 
TATATCCCAG TTCCTGATAT TAACAAACCC CAGTCAACCC ATGCCTTTGC AATGACCTGT 
ATTTGGATTC ATCTCAATAG AAAAGCTCAA AATGACAACT CCAAGCTACA GATTCCAATA 
CCTCATTCCC TAAGACTTCA CCATGAGTTC CTGCAGCAGA GTCTAAGAAA TAAAAGTTTA 
CAGATGAATG ACTATAAGAT TGCTCTATTG TGTAATGCAT ACTCTACAAA TTCAGAATGT 
TTTACATTAC CCATGGGAGC TCTGGTAGAA ACTATTTATG GAAATGGAAT TATGAGGATA 
CCTCTCCCTG GAACAAACTG TATGGCTTCA GGATCTATTA CCCCCTTACC TATGAACCTC 
CTGGATTCAC TGACAGTTCA TGCCAAAATG AGCCTTATTC ACAGCATTGC AACCAGGGTG 
ATAAAACTTG CTCATGCAAA GTCCAGTGTG GCCTTGGCTC CAGCCCTAGT GGAAACTTAC 
AGTCGTTTAT TGGTCTATAT GGAAATAGAG TCTTTGGGCA TCAAAGGATT TATCAGTCAG 
CTTTTGCCAA CTGTTTTCAA ATCACATGCA TGGGGGATCT TACACACACT CCTTGAGATG 
TTTAGCTACC GGATGCATCA TATTCAGCCT CATTACAGAG TTCAGCTCCT GAGTCATCTT 
CATACTTTGG CTGCAGTTGC ACAAACAAAC CAGAACCAGC TCCATCTTTG TGTCGAGAGC 
ACTGCTCTCA GGCTTATAAC AGCATTAGGT AGCTCAGAGG TACAACCGCA GTTTACACGC 
TTCCTTAGTG ATCCCAAAAC AGTGCTCTCA GCAGAATCTG AAGAACTGAA CCGAGCCTTG 
ATATTGACCT TGGCTAGAGC AACTCATGTA ACAGATTTTT TTACAGGCTC TGATTCAATT 
CAGGGAACTT GGTGTAAAGA CATACTTCAG ACCATCATGA GTTTCACTCC TCATAATTGG 
GCTTCACACA CCCTGAGCTG TTTTCCAGGC CCACTACAGG CATTCTTCAA ACAAAATAAT 
GTGCCTCAGG AAAGCCGTTT TAATCTGAAA AAAAATGTGG AGGAGGAGTA TAGGAAGTGG 
AAGTCAATGA GCAACGAAAA CGACATTATT ACCCACTTCT CTATGCAGGG CTCCCCTCCT 
CTCTTTCTTT GTCTTCTCTG GAAAATGCTC TTGGAAACAG ATCATATTAA TCAGATTGGC 
TATAGAGTAT TAGAGAGAAT TGGAGCCAGG GCCTTGGTAG CCCATGTGAG GACATTTGCA 
GATTTCCTGG TATATGAGTT TTCTACATCA GCAGGGGGTC AGCAACTCAA TAAATGCATT 
GAAATTCTTA ATGACATGGT ATGGAAGTAT AACATTGTTA CACTGGACAG ATTAATTCTC 
TGCCTGGCCA TGCGTAGTCA CGAAGGAAAT GAAGCCCAGG TTTGTTATTT CATAATTCAG 
TTGCTGTTAC TCAAACCAAA CGATTTTAGA AATCGAGTAA GTGACTTTGT GAAGGAAAAT 
TCCCCAGAGC ACTGGTTACA GAATGACTGG CACACCAAGC ACATGAATTA TCACAAGAAA 
TATCCAGAGA AGTTGTATTT TGAGGGCCTC GCGGAACAGG TGGATCCTCC TGTACAGATC 
CAGTCTCCCT ATCTGCCCAT CTATTTTGGG AATGTGTGTC TTCGATTCCT TCCAGTATTT 
GATATAGTAA TCCACAGATT TTTAGAGTTG CTTCCGGTAT CCAAATCACT GGAGACTCTA 
CTGGATCATC TAGGAGGCTT ATATAAATTT CATGATCGTC CAGTGACTTA TCTGTATAAC 
ACTCTGCACT ATTATGAAAT GCACCTGAGA GACCGCGCAT TTCTCAAACG AAAACTCGTC 
CATGCGATCA TTGGCTCTCT GAAGGATAAT CGACCGCAGG GCTGGTGTCT AAGTGACACT 
TACCTGAAAT GCGCTATGAA TGCACGAGAG GAAAATCCTT GGGTTCCAGA TGACACCTAC 
TATTGCAGAT TGATTGGCAG ACTAGTCGAT ACGATGGCTG GCAAATCTCC TGGTCCCTTT 
CCAAACTGTG ACTGGAGATT CAATGAGTTT CCCAACCCAG CTGCCCATGC TCTCCATGTT 
ACTTGTGTGG AGCTCATGGC CTTGGCAGTT TCAGGCAAAG AAGTTGGGAA TGCCCTTCTA 
AATGTTGTCC TAAAAAGTCA GCCTTTAGTG CCAAGAGAGA ACATTACAGC ATGGATGAAT 
GCAATTGGTT TGATCATCAC TGCCCTACCA GAGCCATATT GGATTGTTCT TCATGATCGA 
ATTGTGAGTG TCATCAGCAG CCCCAGCTTG ACGTCTGAAA CAGAGTGGGT TGGCTATCCA 
TTCCGCCTCT TTGATTTCAC TGCCTGTCAT CAGTCCTACT CTGAGATGAG TTGTAGCTAT 
ACGTTAGCTC TTGCACATGC TGTGTGGCAC CATTCTAGCA TCGGACAACT TTCTCTCATT 
CCAAAGTTTC TTACTGAAGT ACTTCTTCCT ATAGTGAAGA CCGAATTCCA GTTGCTTTAT 
GTATACCATC TTGTTGGACC ATTTTTACAA AGATTTCAGC AAGAGAGAAC TCGTTGTATG 
ATAGAGATTG GTGTGGCGTT TTATGACATG CTGCTGAATG TTGACCAGTG TAGCACCCAT 
TTAAATTACA TGGATCCCAT CTGTGACTTC CTCTATCACA TGAAGTATAT GTTTACTGGT 
GACAGCGTGA AAGAGCAAGT AGAGAAGATT ATCTGTAACT TAAAACCAGC TTTAAAACTT 
CGTCTTCGAT TCATCACACA CATTAGCAAG ATGGAGCCAG CTGCAGTGCC TCCACAAGCC 
ATGAACAGTG GGTCTCCAGC ACCTCAGTCT AATCAGGTGC CCGTGTCTTT ACCAGTAACT 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( NM_001376524.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363453.1 Homo sapiens mediator complex subunit 23 (MED23), transcript variant 12, mRNA.

Price & Availability↑

CloneID	OHu109130
Clone ID Related Accession (Same CDS sequence)	NM_001376524.1
Accession Version	NM_001376524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform l
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121575.24. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.243722.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG 
GAGGACCAGT TGGTGGATCT GGTTGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG 
TTTGACGATG GGGGAACAAG CCAACTCCTG TGGCAGCATC TCTCAAGTCA GCTCATTTTC 
TTTGTGCTTT TCCAGTTTGC AAGTTTTCCA CATATGGTGC TTTCTCTTCA TCAGAAGTTA 
GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCCT GCAATTCATT 
TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTCC CTGTGATGAA GCTCTTCGAC 
TTGCTATACC CAGAAAAAGA ATATATCCCA GTTCCTGATA TTAACAAACC CCAGTCAACC 
CATGCCTTTG CAATGACCTG TATTTGGATT CATCTCAATA GAAAAGCTCA AAATGACAAC 
TCCAAGCTAC AGATTCCAAT ACCTCATTCC CTAAGACTTC ACCATGAGTT CCTGCAGCAG 
AGTCTAAGAA ATAAAAGTTT ACAGATGAAT GACTATAAGA TTGCTCTATT GTGTAATGCA 
TACTCTACAA ATTCAGAATG TTTTACATTA CCCATGGGAG CTCTGGTAGA AACTATTTAT 
GGAAATGGAA TTATGAGGAT ACCTCTCCCT GGAACAAACT GTATGGCTTC AGGATCTATT 
ACCCCCTTAC CTATGAACCT CCTGGATTCA CTGACAGTTC ATGCCAAAAT GAGCCTTATT 
CACAGCATTG CAACCAGGGT GATAAAACTT GCTCATGCAA AGTCCAGTGT GGCCTTGGCT 
CCAGCCCTAG TGGAAACTTA CAGTCGTTTA TTGGTCTATA TGGAAATAGA GTCTTTGGGC 
ATCAAAGGAT TTATCAGTCA GCTTTTGCCA ACTGTTTTCA AATCACATGC ATGGGGGATC 
TTACACACAC TCCTTGAGAT GTTTAGCTAC CGGATGCATC ATATTCAGCC TCATTACAGA 
GTTCAGCTCC TGAGTCATCT TCATACTTTG GCTGCAGTTG CACAAACAAA CCAGAACCAG 
CTCCATCTTT GTGTCGAGAG CACTGCTCTC AGGCTTATAA CAGCATTAGG TAGCTCAGAG 
GTACAACCGC AGTTTACACG CTTCCTTAGT GATCCCAAAA CAGTGCTCTC AGCAGAATCT 
GAAGAACTGA ACCGAGCCTT GATATTGACC TTGGCTAGAG CAACTCATGT AACAGATTTT 
TTTACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG 
AGTTTCACTC CTCATAATTG GGCTTCACAC ACCCTGAGCT GTTTTCCAGG CCCACTACAG 
GCCATGCGTA GTCACGAAGG AAATGAAGCC CAGGTTTGTT ATTTCATAAT TCAGTTGCTG 
TTACTCAAAC CAAACGATTT TAGAAATCGA GTAAGTGACT TTGTGAAGGA AAATTCCCCA 
GAGCACTGGT TACAGAATGA CTGGCACACC AAGCACATGA ATTATCACAA GAAATATCCA 
GAGAAGTTGT ATTTTGAGGG CCTCGCGGAA CAGGTGGATC CTCCTGTACA GATCCAGTCT 
CCCTATCTGC CCATCTATTT TGGGAATGTG TGTCTTCGAT TCCTTCCAGT ATTTGATATA 
GTAATCCACA GATTTTTAGA GTTGCTTCCG GTATCCAAAT CACTGGAGAC TCTACTGGAT 
CATCTAGGAG GCTTATATAA ATTTCATGAT CGTCCAGTGA CTTATCTGTA TAACACTCTG 
CACTATTATG AAATGCACCT GAGAGACCGC GCATTTCTCA AACGAAAACT CGTCCATGCG 
ATCATTGGCT CTCTGAAGGA TAATCGACCG CAGGGCTGGT GTCTAAGTGA CACTTACCTG 
AAATGCGCTA TGAATGCACG AGAGGAAAAT CCTTGGGTTC CAGATGACAC CTACTATTGC 
AGATTGATTG GCAGACTAGT CGATACGATG GCTGGCAAAT CTCCTGGTCC CTTTCCAAAC 
TGTGACTGGA GATTCAATGA GTTTCCCAAC CCAGCTGCCC ATGCTCTCCA TGTTACTTGT 
GTGGAGCTCA TGGCCTTGGC AGTTTCAGGC AAAGAAGTTG GGAATGCCCT TCTAAATGTT 
GTCCTAAAAA GTCAGCCTTT AGTGCCAAGA GAGAACATTA CAGCATGGAT GAATGCAATT 
GGTTTGATCA TCACTGCCCT ACCAGAGCCA TATTGGATTG TTCTTCATGA TCGAATTGTG 
AGTGTCATCA GCAGCCCCAG CTTGACGTCT GAAACAGAGT GGGTTGGCTA TCCATTCCGC 
CTCTTTGATT TCACTGCCTG TCATCAGTCC TACTCTGAGA TGAGTTGTAG CTATACGTTA 
GCTCTTGCAC ATGCTGTGTG GCACCATTCT AGCATCGGAC AACTTTCTCT CATTCCAAAG 
TTTCTTACTG AAGTACTTCT TCCTATAGTG AAGACCGAAT TCCAGTTGCT TTATGTATAC 
CATCTTGTTG GACCATTTTT ACAAAGATTT CAGCAAGAGA GAACTCGTTG TATGATAGAG 
ATTGGTGTGG CGTTTTATGA CATGCTGCTG AATGTTGACC AGTGTAGCAC CCATTTAAAT 
TACATGGATC CCATCTGTGA CTTCCTCTAT CACATGAAGT ATATGTTTAC TGGTGACAGC 
GTGAAAGAGC AAGTAGAGAA GATTATCTGT AACTTAAAAC CAGCTTTAAA ACTTCGTCTT 
CGATTCATCA CACACATTAG CAAGATGGAG CCAGCTGCAG TGCCTCCACA AGCCATGAAC 
AGTGGGTCTC CAGCACCTCA GTCTAATCAG GTGCCCGTGT CTTTACCAGT AACTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363453.1
CDS	194..3913
Translation	METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQVPVSLPVTQ

Target ORF information:

RefSeq Version	NM_001376524.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376524.1

ORF Insert Sequence:

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG 
GAGGACCAGT TGGTGGATCT GGTTGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG 
TTTGACGATG GGGGAACAAG CCAACTCCTG TGGCAGCATC TCTCAAGTCA GCTCATTTTC 
TTTGTGCTTT TCCAGTTTGC AAGTTTTCCA CATATGGTGC TTTCTCTTCA TCAGAAGTTA 
GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCCT GCAATTCATT 
TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTCC CTGTGATGAA GCTCTTCGAC 
TTGCTATACC CAGAAAAAGA ATATATCCCA GTTCCTGATA TTAACAAACC CCAGTCAACC 
CATGCCTTTG CAATGACCTG TATTTGGATT CATCTCAATA GAAAAGCTCA AAATGACAAC 
TCCAAGCTAC AGATTCCAAT ACCTCATTCC CTAAGACTTC ACCATGAGTT CCTGCAGCAG 
AGTCTAAGAA ATAAAAGTTT ACAGATGAAT GACTATAAGA TTGCTCTATT GTGTAATGCA 
TACTCTACAA ATTCAGAATG TTTTACATTA CCCATGGGAG CTCTGGTAGA AACTATTTAT 
GGAAATGGAA TTATGAGGAT ACCTCTCCCT GGAACAAACT GTATGGCTTC AGGATCTATT 
ACCCCCTTAC CTATGAACCT CCTGGATTCA CTGACAGTTC ATGCCAAAAT GAGCCTTATT 
CACAGCATTG CAACCAGGGT GATAAAACTT GCTCATGCAA AGTCCAGTGT GGCCTTGGCT 
CCAGCCCTAG TGGAAACTTA CAGTCGTTTA TTGGTCTATA TGGAAATAGA GTCTTTGGGC 
ATCAAAGGAT TTATCAGTCA GCTTTTGCCA ACTGTTTTCA AATCACATGC ATGGGGGATC 
TTACACACAC TCCTTGAGAT GTTTAGCTAC CGGATGCATC ATATTCAGCC TCATTACAGA 
GTTCAGCTCC TGAGTCATCT TCATACTTTG GCTGCAGTTG CACAAACAAA CCAGAACCAG 
CTCCATCTTT GTGTCGAGAG CACTGCTCTC AGGCTTATAA CAGCATTAGG TAGCTCAGAG 
GTACAACCGC AGTTTACACG CTTCCTTAGT GATCCCAAAA CAGTGCTCTC AGCAGAATCT 
GAAGAACTGA ACCGAGCCTT GATATTGACC TTGGCTAGAG CAACTCATGT AACAGATTTT 
TTTACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG 
AGTTTCACTC CTCATAATTG GGCTTCACAC ACCCTGAGCT GTTTTCCAGG CCCACTACAG 
GCCATGCGTA GTCACGAAGG AAATGAAGCC CAGGTTTGTT ATTTCATAAT TCAGTTGCTG 
TTACTCAAAC CAAACGATTT TAGAAATCGA GTAAGTGACT TTGTGAAGGA AAATTCCCCA 
GAGCACTGGT TACAGAATGA CTGGCACACC AAGCACATGA ATTATCACAA GAAATATCCA 
GAGAAGTTGT ATTTTGAGGG CCTCGCGGAA CAGGTGGATC CTCCTGTACA GATCCAGTCT 
CCCTATCTGC CCATCTATTT TGGGAATGTG TGTCTTCGAT TCCTTCCAGT ATTTGATATA 
GTAATCCACA GATTTTTAGA GTTGCTTCCG GTATCCAAAT CACTGGAGAC TCTACTGGAT 
CATCTAGGAG GCTTATATAA ATTTCATGAT CGTCCAGTGA CTTATCTGTA TAACACTCTG 
CACTATTATG AAATGCACCT GAGAGACCGC GCATTTCTCA AACGAAAACT CGTCCATGCG 
ATCATTGGCT CTCTGAAGGA TAATCGACCG CAGGGCTGGT GTCTAAGTGA CACTTACCTG 
AAATGCGCTA TGAATGCACG AGAGGAAAAT CCTTGGGTTC CAGATGACAC CTACTATTGC 
AGATTGATTG GCAGACTAGT CGATACGATG GCTGGCAAAT CTCCTGGTCC CTTTCCAAAC 
TGTGACTGGA GATTCAATGA GTTTCCCAAC CCAGCTGCCC ATGCTCTCCA TGTTACTTGT 
GTGGAGCTCA TGGCCTTGGC AGTTTCAGGC AAAGAAGTTG GGAATGCCCT TCTAAATGTT 
GTCCTAAAAA GTCAGCCTTT AGTGCCAAGA GAGAACATTA CAGCATGGAT GAATGCAATT 
GGTTTGATCA TCACTGCCCT ACCAGAGCCA TATTGGATTG TTCTTCATGA TCGAATTGTG 
AGTGTCATCA GCAGCCCCAG CTTGACGTCT GAAACAGAGT GGGTTGGCTA TCCATTCCGC 
CTCTTTGATT TCACTGCCTG TCATCAGTCC TACTCTGAGA TGAGTTGTAG CTATACGTTA 
GCTCTTGCAC ATGCTGTGTG GCACCATTCT AGCATCGGAC AACTTTCTCT CATTCCAAAG 
TTTCTTACTG AAGTACTTCT TCCTATAGTG AAGACCGAAT TCCAGTTGCT TTATGTATAC 
CATCTTGTTG GACCATTTTT ACAAAGATTT CAGCAAGAGA GAACTCGTTG TATGATAGAG 
ATTGGTGTGG CGTTTTATGA CATGCTGCTG AATGTTGACC AGTGTAGCAC CCATTTAAAT 
TACATGGATC CCATCTGTGA CTTCCTCTAT CACATGAAGT ATATGTTTAC TGGTGACAGC 
GTGAAAGAGC AAGTAGAGAA GATTATCTGT AACTTAAAAC CAGCTTTAAA ACTTCGTCTT 
CGATTCATCA CACACATTAG CAAGATGGAG CCAGCTGCAG TGCCTCCACA AGCCATGAAC 
AGTGGGTCTC CAGCACCTCA GTCTAATCAG GTGCCCGTGT CTTTACCAGT AACTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( NM_001376519.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363448.1 Homo sapiens mediator complex subunit 23 (MED23), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu109131
Clone ID Related Accession (Same CDS sequence)	NM_001376519.1
Accession Version	NM_001376519.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121575.24. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.109595.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG 
GAGGACCAGT TGGTGGATCT GGTTGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG 
TTTGACGATG GGGGAACAAG CCAACTCCTG TGGCAGCATC TCTCAAGTCA GCTCATTTTC 
TTTGTGCTTT TCCAGTTTGC AAGTTTTCCA CATATGGTGC TTTCTCTTCA TCAGAAGTTA 
GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCCT GCAATTCATT 
TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTCC CTGTGATGAA GCTCTTCGAC 
TTGCTATACC CAGAAAAAGA ATATATCCCA GTTCCTGATA TTAACAAACC CCAGTCAACC 
CATGCCTTTG CAATGACCTG TATTTGGATT CATCTCAATA GAAAAGCTCA AAATGACAAC 
TCCAAGCTAC AGATTCCAAT ACCTCATTCC CTAAGACTTC ACCATGAGTT CCTGCAGCAG 
AGTCTAAGAA ATAAAAGTTT ACAGATGAAT GACTATAAGA TTGCTCTATT GTGTAATGCA 
TACTCTACAA ATTCAGAATG TTTTACATTA CCCATGGGAG CTCTGGTAGA AACTATTTAT 
GGAAATGGAA TTATGAGGAT ACCTCTCCCT GGAACAAACT GTATGGCTTC AGGATCTATT 
ACCCCCTTAC CTATGAACCT CCTGGATTCA CTGACAGTTC ATGCCAAAAT GAGCCTTATT 
CACAGCATTG CAACCAGGGT GATAAAACTT GCTCATGCAA AGTCCAGTGT GGCCTTGGCT 
CCAGCCCTAG TGGAAACTTA CAGTCGTTTA TTGGTCTATA TGGAAATAGA GTCTTTGGGC 
ATCAAAGGAT TTATCAGTCA GCTTTTGCCA ACTGTTTTCA AATCACATGC ATGGGGGATC 
TTACACACAC TCCTTGAGAT GTTTAGCTAC CGGATGCATC ATATTCAGCC TCATTACAGA 
GTTCAGCTCC TGAGTCATCT TCATACTTTG GCTGCAGTTG CACAAACAAA CCAGAACCAG 
CTCCATCTTT GTGTCGAGAG CACTGCTCTC AGGCTTATAA CAGCATTAGG TAGCTCAGAG 
GTACAACCGC AGTTTACACG CTTCCTTAGT GATCCCAAAA CAGTGCTCTC AGCAGAATCT 
GAAGAACTGA ACCGAGCCTT GATATTGACC TTGGCTAGAG CAACTCATGT AACAGATTTT 
TTTACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG 
AGTTTCACTC CTCATAATTG GGCTTCACAC ACCCTGAGCT GTTTTCCAGG CCCACTACAG 
GCATTCTTCA AACAAAATAA TGTGCCTCAG GAAAGCCGTT TTAATCTGAA AAAAAATGTG 
GAGGAGGAGT ATAGGAAGTG GAAGTCAATG AGCAACGAAA ACGACATTAT TACCCACTTC 
TCTATGCAGG GCTCCCCTCC TCTCTTTCTT TGTCTTCTCT GGAAAATGCT CTTGGAAACA 
GATCATATTA ATCAGATTGG CTATAGAGTA TTAGAGAGAA TTGGAGCCAG GGCCTTGGTA 
GCCCATGTGA GGACATTTGC AGATTTCCTG GTATATGAGT TTTCTACATC AGCAGGGGGT 
CAGCAACTCA ATAAATGCAT TGAAATTCTT AATGACATGG TATGGAAGTA TAACATTGTT 
ACACTGGACA GATTAATTCT CTGCCTGGCC ATGCGTAGTC ACGAAGGAAA TGAAGCCCAG 
AAATATCCAG AGAAGTTGTA TTTTGAGGGC CTCGCGGAAC AGGTGGATCC TCCTGTACAG 
ATCCAGTCTC CCTATCTGCC CATCTATTTT GGGAATGTGT GTCTTCGATT CCTTCCAGTA 
TTTGATATAG TAATCCACAG ATTTTTAGAG TTGCTTCCGG TATCCAAATC ACTGGAGACT 
CTACTGGATC ATCTAGGAGG CTTATATAAA TTTCATGATC GTCCAGTGAC TTATCTGTAT 
AACACTCTGC ACTATTATGA AATGCACCTG AGAGACCGCG CATTTCTCAA ACGAAAACTC 
GTCCATGCGA TCATTGGCTC TCTGAAGGAT AATCGACCGC AGGGCTGGTG TCTAAGTGAC 
ACTTACCTGA AATGCGCTAT GAATGCACGA GAGGAAAATC CTTGGGTTCC AGATGACACC 
TACTATTGCA GATTGATTGG CAGACTAGTC GATACGATGG CTGGCAAATC TCCTGGTCCC 
TTTCCAAACT GTGACTGGAG ATTCAATGAG TTTCCCAACC CAGCTGCCCA TGCTCTCCAT 
GTTACTTGTG TGGAGCTCAT GGCCTTGGCA GTTTCAGGCA AAGAAGTTGG GAATGCCCTT 
CTAAATGTTG TCCTAAAAAG TCAGCCTTTA GTGCCAAGAG AGAACATTAC AGCATGGATG 
AATGCAATTG GTTTGATCAT CACTGCCCTA CCAGAGCCAT ATTGGATTGT TCTTCATGAT 
CGAATTGTGA GTGTCATCAG CAGCCCCAGC TTGACGTCTG AAACAGAGTG GGTTGGCTAT 
CCATTCCGCC TCTTTGATTT CACTGCCTGT CATCAGTCCT ACTCTGAGAT GAGTTGTAGC 
TATACGTTAG CTCTTGCACA TGCTGTGTGG CACCATTCTA GCATCGGACA ACTTTCTCTC 
ATTCCAAAGT TTCTTACTGA AGTACTTCTT CCTATAGTGA AGACCGAATT CCAGTTGCTT 
TATGTATACC ATCTTGTTGG ACCATTTTTA CAAAGATTTC AGCAAGAGAG AACTCGTTGT 
ATGATAGAGA TTGGTGTGGC GTTTTATGAC ATGCTGCTGA ATGTTGACCA GTGTAGCACC 
CATTTAAATT ACATGGATCC CATCTGTGAC TTCCTCTATC ACATGAAGTA TATGTTTACT 
GGTGACAGCG TGAAAGAGCA AGTAGAGAAG ATTATCTGTA ACTTAAAACC AGCTTTAAAA 
CTTCGTCTTC GATTCATCAC ACACATTAGC AAGATGGAGC CAGCTGCAGT GCCTCCACAA 
GCCATGAACA GTGGGTCTCC AGCACCTCAG TCTAATCAGG TGCCCGTGTC TTTACCAGTA 
ACTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363448.1
CDS	194..4162
Translation	METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQVPVSLPVTQ

Target ORF information:

RefSeq Version	NM_001376519.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376519.1

ORF Insert Sequence:

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG 
GAGGACCAGT TGGTGGATCT GGTTGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG 
TTTGACGATG GGGGAACAAG CCAACTCCTG TGGCAGCATC TCTCAAGTCA GCTCATTTTC 
TTTGTGCTTT TCCAGTTTGC AAGTTTTCCA CATATGGTGC TTTCTCTTCA TCAGAAGTTA 
GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCCT GCAATTCATT 
TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTCC CTGTGATGAA GCTCTTCGAC 
TTGCTATACC CAGAAAAAGA ATATATCCCA GTTCCTGATA TTAACAAACC CCAGTCAACC 
CATGCCTTTG CAATGACCTG TATTTGGATT CATCTCAATA GAAAAGCTCA AAATGACAAC 
TCCAAGCTAC AGATTCCAAT ACCTCATTCC CTAAGACTTC ACCATGAGTT CCTGCAGCAG 
AGTCTAAGAA ATAAAAGTTT ACAGATGAAT GACTATAAGA TTGCTCTATT GTGTAATGCA 
TACTCTACAA ATTCAGAATG TTTTACATTA CCCATGGGAG CTCTGGTAGA AACTATTTAT 
GGAAATGGAA TTATGAGGAT ACCTCTCCCT GGAACAAACT GTATGGCTTC AGGATCTATT 
ACCCCCTTAC CTATGAACCT CCTGGATTCA CTGACAGTTC ATGCCAAAAT GAGCCTTATT 
CACAGCATTG CAACCAGGGT GATAAAACTT GCTCATGCAA AGTCCAGTGT GGCCTTGGCT 
CCAGCCCTAG TGGAAACTTA CAGTCGTTTA TTGGTCTATA TGGAAATAGA GTCTTTGGGC 
ATCAAAGGAT TTATCAGTCA GCTTTTGCCA ACTGTTTTCA AATCACATGC ATGGGGGATC 
TTACACACAC TCCTTGAGAT GTTTAGCTAC CGGATGCATC ATATTCAGCC TCATTACAGA 
GTTCAGCTCC TGAGTCATCT TCATACTTTG GCTGCAGTTG CACAAACAAA CCAGAACCAG 
CTCCATCTTT GTGTCGAGAG CACTGCTCTC AGGCTTATAA CAGCATTAGG TAGCTCAGAG 
GTACAACCGC AGTTTACACG CTTCCTTAGT GATCCCAAAA CAGTGCTCTC AGCAGAATCT 
GAAGAACTGA ACCGAGCCTT GATATTGACC TTGGCTAGAG CAACTCATGT AACAGATTTT 
TTTACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG 
AGTTTCACTC CTCATAATTG GGCTTCACAC ACCCTGAGCT GTTTTCCAGG CCCACTACAG 
GCATTCTTCA AACAAAATAA TGTGCCTCAG GAAAGCCGTT TTAATCTGAA AAAAAATGTG 
GAGGAGGAGT ATAGGAAGTG GAAGTCAATG AGCAACGAAA ACGACATTAT TACCCACTTC 
TCTATGCAGG GCTCCCCTCC TCTCTTTCTT TGTCTTCTCT GGAAAATGCT CTTGGAAACA 
GATCATATTA ATCAGATTGG CTATAGAGTA TTAGAGAGAA TTGGAGCCAG GGCCTTGGTA 
GCCCATGTGA GGACATTTGC AGATTTCCTG GTATATGAGT TTTCTACATC AGCAGGGGGT 
CAGCAACTCA ATAAATGCAT TGAAATTCTT AATGACATGG TATGGAAGTA TAACATTGTT 
ACACTGGACA GATTAATTCT CTGCCTGGCC ATGCGTAGTC ACGAAGGAAA TGAAGCCCAG 
AAATATCCAG AGAAGTTGTA TTTTGAGGGC CTCGCGGAAC AGGTGGATCC TCCTGTACAG 
ATCCAGTCTC CCTATCTGCC CATCTATTTT GGGAATGTGT GTCTTCGATT CCTTCCAGTA 
TTTGATATAG TAATCCACAG ATTTTTAGAG TTGCTTCCGG TATCCAAATC ACTGGAGACT 
CTACTGGATC ATCTAGGAGG CTTATATAAA TTTCATGATC GTCCAGTGAC TTATCTGTAT 
AACACTCTGC ACTATTATGA AATGCACCTG AGAGACCGCG CATTTCTCAA ACGAAAACTC 
GTCCATGCGA TCATTGGCTC TCTGAAGGAT AATCGACCGC AGGGCTGGTG TCTAAGTGAC 
ACTTACCTGA AATGCGCTAT GAATGCACGA GAGGAAAATC CTTGGGTTCC AGATGACACC 
TACTATTGCA GATTGATTGG CAGACTAGTC GATACGATGG CTGGCAAATC TCCTGGTCCC 
TTTCCAAACT GTGACTGGAG ATTCAATGAG TTTCCCAACC CAGCTGCCCA TGCTCTCCAT 
GTTACTTGTG TGGAGCTCAT GGCCTTGGCA GTTTCAGGCA AAGAAGTTGG GAATGCCCTT 
CTAAATGTTG TCCTAAAAAG TCAGCCTTTA GTGCCAAGAG AGAACATTAC AGCATGGATG 
AATGCAATTG GTTTGATCAT CACTGCCCTA CCAGAGCCAT ATTGGATTGT TCTTCATGAT 
CGAATTGTGA GTGTCATCAG CAGCCCCAGC TTGACGTCTG AAACAGAGTG GGTTGGCTAT 
CCATTCCGCC TCTTTGATTT CACTGCCTGT CATCAGTCCT ACTCTGAGAT GAGTTGTAGC 
TATACGTTAG CTCTTGCACA TGCTGTGTGG CACCATTCTA GCATCGGACA ACTTTCTCTC 
ATTCCAAAGT TTCTTACTGA AGTACTTCTT CCTATAGTGA AGACCGAATT CCAGTTGCTT 
TATGTATACC ATCTTGTTGG ACCATTTTTA CAAAGATTTC AGCAAGAGAG AACTCGTTGT 
ATGATAGAGA TTGGTGTGGC GTTTTATGAC ATGCTGCTGA ATGTTGACCA GTGTAGCACC 
CATTTAAATT ACATGGATCC CATCTGTGAC TTCCTCTATC ACATGAAGTA TATGTTTACT 
GGTGACAGCG TGAAAGAGCA AGTAGAGAAG ATTATCTGTA ACTTAAAACC AGCTTTAAAA 
CTTCGTCTTC GATTCATCAC ACACATTAGC AAGATGGAGC CAGCTGCAGT GCCTCCACAA 
GCCATGAACA GTGGGTCTCC AGCACCTCAG TCTAATCAGG TGCCCGTGTC TTTACCAGTA 
ACTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( NM_001376523.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363452.1 Homo sapiens mediator complex subunit 23 (MED23), transcript variant 11, mRNA.

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CloneID	OHu109136
Clone ID Related Accession (Same CDS sequence)	NM_001376523.1
Accession Version	NM_001376523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform k
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121575.24. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.203324.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
TTTGCAAGTT TTCCACATAT GGTGCTTTCT CTTCATCAGA AGTTAGCAGG GCGAGGACTG 
ATTAAAGGCA GAGATCATCT TATGTGGGTT CTCCTGCAAT TCATTTCTGG AAGTATTCAG 
AAAAATGCAC TAGCTGATTT TCTCCCTGTG ATGAAGCTCT TCGACTTGCT ATACCCAGAA 
AAAGAATATA TCCCAGTTCC TGATATTAAC AAACCCCAGT CAACCCATGC CTTTGCAATG 
ACCTGTATTT GGATTCATCT CAATAGAAAA GCTCAAAATG ACAACTCCAA GCTACAGATT 
CCAATACCTC ATTCCCTAAG ACTTCACCAT GAGTTCCTGC AGCAGAGTCT AAGAAATAAA 
AGTTTACAGA TGAATGACTA TAAGATTGCT CTATTGTGTA ATGCATACTC TACAAATTCA 
GAATGTTTTA CATTACCCAT GGGAGCTCTG GTAGAAACTA TTTATGGAAA TGGAATTATG 
AGGATACCTC TCCCTGGAAC AAACTGTATG GCTTCAGGAT CTATTACCCC CTTACCTATG 
AACCTCCTGG ATTCACTGAC AGTTCATGCC AAAATGAGCC TTATTCACAG CATTGCAACC 
AGGGTGATAA AACTTGCTCA TGCAAAGTCC AGTGTGGCCT TGGCTCCAGC CCTAGTGGAA 
ACTTACAGTC GTTTATTGGT CTATATGGAA ATAGAGTCTT TGGGCATCAA AGGATTTATC 
AGTCAGCTTT TGCCAACTGT TTTCAAATCA CATGCATGGG GGATCTTACA CACACTCCTT 
GAGATGTTTA GCTACCGGAT GCATCATATT CAGCCTCATT ACAGAGTTCA GCTCCTGAGT 
CATCTTCATA CTTTGGCTGC AGTTGCACAA ACAAACCAGA ACCAGCTCCA TCTTTGTGTC 
GAGAGCACTG CTCTCAGGCT TATAACAGCA TTAGGTAGCT CAGAGGTACA ACCGCAGTTT 
ACACGCTTCC TTAGTGATCC CAAAACAGTG CTCTCAGCAG AATCTGAAGA ACTGAACCGA 
GCCTTGATAT TGACCTTGGC TAGAGCAACT CATGTAACAG ATTTTTTTAC AGGCTCTGAT 
TCAATTCAGG GAACTTGGTG TAAAGACATA CTTCAGACCA TCATGAGTTT CACTCCTCAT 
AATTGGGCTT CACACACCCT GAGCTGTTTT CCAGGCCCAC TACAGGCATT CTTCAAACAA 
AATAATGTGC CTCAGGAAAG CCGTTTTAAT CTGAAAAAAA ATGTGGAGGA GGAGTATAGG 
AAGTGGAAGT CAATGAGCAA CGAAAACGAC ATTATTACCC ACTTCTCTAT GCAGGGCTCC 
CCTCCTCTCT TTCTTTGTCT TCTCTGGAAA ATGCTCTTGG AAACAGATCA TATTAATCAG 
ATTGGCTATA GAGTATTAGA GAGAATTGGA GCCAGGGCCT TGGTAGCCCA TGTGAGGACA 
TTTGCAGATT TCCTGGTATA TGAGTTTTCT ACATCAGCAG GGGGTCAGCA ACTCAATAAA 
TGCATTGAAA TTCTTAATGA CATGGTATGG AAGTATAACA TTGTTACACT GGACAGATTA 
ATTCTCTGCC TGGCCATGCG TAGTCACGAA GGAAATGAAG CCCAGGTTTG TTATTTCATA 
ATTCAGTTGC TGTTACTCAA ACCAAACGAT TTTAGAAATC GAGTAAGTGA CTTTGTGAAG 
GAAAATTCCC CAGAGCACTG GTTACAGAAT GACTGGCACA CCAAGCACAT GAATTATCAC 
AAGAAATATC CAGAGAAGTT GTATTTTGAG GGCCTCGCGG AACAGGTGGA TCCTCCTGTA 
CAGATCCAGT CTCCCTATCT GCCCATCTAT TTTGGGAATG TGTGTCTTCG ATTCCTTCCA 
GTATTTGATA TAGTAATCCA CAGATTTTTA GAGTTGCTTC CGGTATCCAA ATCACTGGAG 
ACTCTACTGG ATCATCTAGG AGGCTTATAT AAATTTCATG ATCGTCCAGT GACTTATCTG 
TATAACACTC TGCACTATTA TGAAATGCAC CTGAGAGACC GCGCATTTCT CAAACGAAAA 
CTCGTCCATG CGATCATTGG CTCTCTGAAG GATAATCGAC CGCAGGGCTG GTGTCTAAGT 
GACACTTACC TGAAATGCGC TATGAATGCA CGAGAGGAAA ATCCTTGGGT TCCAGATGAC 
ACCTACTATT GCAGATTGAT TGGCAGACTA GTCGATACGA TGGCTGGCAA ATCTCCTGGT 
CCCTTTCCAA ACTGTGACTG GAGATTCAAT GAGTTTCCCA ACCCAGCTGC CCATGCTCTC 
CATGTTACTT GTGTGGAGCT CATGGCCTTG GCAGTTTCAG GCAAAGAAGT TGGGAATGCC 
CTTCTAAATG TTGTCCTAAA AAGTCAGCCT TTAGTGCCAA GAGAGAACAT TACAGCATGG 
ATGAATGCAA TTGGTTTGAT CATCACTGCC CTACCAGAGC CATATTGGAT TGTTCTTCAT 
GATCGAATTG TGAGTGTCAT CAGCAGCCCC AGCTTGACGT CTGAAACAGA GTGGGTTGGC 
TATCCATTCC GCCTCTTTGA TTTCACTGCC TGTCATCAGT CCTACTCTGA GATGAGTTGT 
AGCTATACGT TAGCTCTTGC ACATGCTGTG TGGCACCATT CTAGCATCGG ACAACTTTCT 
CTCATTCCAA AGTTTCTTAC TGAAGTACTT CTTCCTATAG TGAAGACCGA ATTCCAGTTG 
CTTTATGTAT ACCATCTTGT TGGACCATTT TTACAAAGAT TTCAGCAAGA GAGAACTCGT 
TGTATGATAG AGATTGGTGT GGCGTTTTAT GACATGCTGC TGAATGTTGA CCAGTGTAGC 
ACCCATTTAA ATTACATGGA TCCCATCTGT GACTTCCTCT ATCACATGAA GTATATGTTT 
ACTGGTGACA GCGTGAAAGA GCAAGTAGAG AAGATTATCT GTAACTTAAA ACCAGCTTTA 
AAACTTCGTC TTCGATTCAT CACACACATT AGCAAGATGG AGCCAGCTGC AGTGCCTCCA 
CAAGCCATGA ACAGTGGGTC TCCAGCACCT CAGTCTAATC AGGTGCCCGT GTCTTTACCA 
GTAACTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363452.1
CDS	194..4045
Translation	METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQVPVSLPVTQ

Target ORF information:

RefSeq Version	NM_001376523.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376523.1

ORF Insert Sequence:

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
TTTGCAAGTT TTCCACATAT GGTGCTTTCT CTTCATCAGA AGTTAGCAGG GCGAGGACTG 
ATTAAAGGCA GAGATCATCT TATGTGGGTT CTCCTGCAAT TCATTTCTGG AAGTATTCAG 
AAAAATGCAC TAGCTGATTT TCTCCCTGTG ATGAAGCTCT TCGACTTGCT ATACCCAGAA 
AAAGAATATA TCCCAGTTCC TGATATTAAC AAACCCCAGT CAACCCATGC CTTTGCAATG 
ACCTGTATTT GGATTCATCT CAATAGAAAA GCTCAAAATG ACAACTCCAA GCTACAGATT 
CCAATACCTC ATTCCCTAAG ACTTCACCAT GAGTTCCTGC AGCAGAGTCT AAGAAATAAA 
AGTTTACAGA TGAATGACTA TAAGATTGCT CTATTGTGTA ATGCATACTC TACAAATTCA 
GAATGTTTTA CATTACCCAT GGGAGCTCTG GTAGAAACTA TTTATGGAAA TGGAATTATG 
AGGATACCTC TCCCTGGAAC AAACTGTATG GCTTCAGGAT CTATTACCCC CTTACCTATG 
AACCTCCTGG ATTCACTGAC AGTTCATGCC AAAATGAGCC TTATTCACAG CATTGCAACC 
AGGGTGATAA AACTTGCTCA TGCAAAGTCC AGTGTGGCCT TGGCTCCAGC CCTAGTGGAA 
ACTTACAGTC GTTTATTGGT CTATATGGAA ATAGAGTCTT TGGGCATCAA AGGATTTATC 
AGTCAGCTTT TGCCAACTGT TTTCAAATCA CATGCATGGG GGATCTTACA CACACTCCTT 
GAGATGTTTA GCTACCGGAT GCATCATATT CAGCCTCATT ACAGAGTTCA GCTCCTGAGT 
CATCTTCATA CTTTGGCTGC AGTTGCACAA ACAAACCAGA ACCAGCTCCA TCTTTGTGTC 
GAGAGCACTG CTCTCAGGCT TATAACAGCA TTAGGTAGCT CAGAGGTACA ACCGCAGTTT 
ACACGCTTCC TTAGTGATCC CAAAACAGTG CTCTCAGCAG AATCTGAAGA ACTGAACCGA 
GCCTTGATAT TGACCTTGGC TAGAGCAACT CATGTAACAG ATTTTTTTAC AGGCTCTGAT 
TCAATTCAGG GAACTTGGTG TAAAGACATA CTTCAGACCA TCATGAGTTT CACTCCTCAT 
AATTGGGCTT CACACACCCT GAGCTGTTTT CCAGGCCCAC TACAGGCATT CTTCAAACAA 
AATAATGTGC CTCAGGAAAG CCGTTTTAAT CTGAAAAAAA ATGTGGAGGA GGAGTATAGG 
AAGTGGAAGT CAATGAGCAA CGAAAACGAC ATTATTACCC ACTTCTCTAT GCAGGGCTCC 
CCTCCTCTCT TTCTTTGTCT TCTCTGGAAA ATGCTCTTGG AAACAGATCA TATTAATCAG 
ATTGGCTATA GAGTATTAGA GAGAATTGGA GCCAGGGCCT TGGTAGCCCA TGTGAGGACA 
TTTGCAGATT TCCTGGTATA TGAGTTTTCT ACATCAGCAG GGGGTCAGCA ACTCAATAAA 
TGCATTGAAA TTCTTAATGA CATGGTATGG AAGTATAACA TTGTTACACT GGACAGATTA 
ATTCTCTGCC TGGCCATGCG TAGTCACGAA GGAAATGAAG CCCAGGTTTG TTATTTCATA 
ATTCAGTTGC TGTTACTCAA ACCAAACGAT TTTAGAAATC GAGTAAGTGA CTTTGTGAAG 
GAAAATTCCC CAGAGCACTG GTTACAGAAT GACTGGCACA CCAAGCACAT GAATTATCAC 
AAGAAATATC CAGAGAAGTT GTATTTTGAG GGCCTCGCGG AACAGGTGGA TCCTCCTGTA 
CAGATCCAGT CTCCCTATCT GCCCATCTAT TTTGGGAATG TGTGTCTTCG ATTCCTTCCA 
GTATTTGATA TAGTAATCCA CAGATTTTTA GAGTTGCTTC CGGTATCCAA ATCACTGGAG 
ACTCTACTGG ATCATCTAGG AGGCTTATAT AAATTTCATG ATCGTCCAGT GACTTATCTG 
TATAACACTC TGCACTATTA TGAAATGCAC CTGAGAGACC GCGCATTTCT CAAACGAAAA 
CTCGTCCATG CGATCATTGG CTCTCTGAAG GATAATCGAC CGCAGGGCTG GTGTCTAAGT 
GACACTTACC TGAAATGCGC TATGAATGCA CGAGAGGAAA ATCCTTGGGT TCCAGATGAC 
ACCTACTATT GCAGATTGAT TGGCAGACTA GTCGATACGA TGGCTGGCAA ATCTCCTGGT 
CCCTTTCCAA ACTGTGACTG GAGATTCAAT GAGTTTCCCA ACCCAGCTGC CCATGCTCTC 
CATGTTACTT GTGTGGAGCT CATGGCCTTG GCAGTTTCAG GCAAAGAAGT TGGGAATGCC 
CTTCTAAATG TTGTCCTAAA AAGTCAGCCT TTAGTGCCAA GAGAGAACAT TACAGCATGG 
ATGAATGCAA TTGGTTTGAT CATCACTGCC CTACCAGAGC CATATTGGAT TGTTCTTCAT 
GATCGAATTG TGAGTGTCAT CAGCAGCCCC AGCTTGACGT CTGAAACAGA GTGGGTTGGC 
TATCCATTCC GCCTCTTTGA TTTCACTGCC TGTCATCAGT CCTACTCTGA GATGAGTTGT 
AGCTATACGT TAGCTCTTGC ACATGCTGTG TGGCACCATT CTAGCATCGG ACAACTTTCT 
CTCATTCCAA AGTTTCTTAC TGAAGTACTT CTTCCTATAG TGAAGACCGA ATTCCAGTTG 
CTTTATGTAT ACCATCTTGT TGGACCATTT TTACAAAGAT TTCAGCAAGA GAGAACTCGT 
TGTATGATAG AGATTGGTGT GGCGTTTTAT GACATGCTGC TGAATGTTGA CCAGTGTAGC 
ACCCATTTAA ATTACATGGA TCCCATCTGT GACTTCCTCT ATCACATGAA GTATATGTTT 
ACTGGTGACA GCGTGAAAGA GCAAGTAGAG AAGATTATCT GTAACTTAAA ACCAGCTTTA 
AAACTTCGTC TTCGATTCAT CACACACATT AGCAAGATGG AGCCAGCTGC AGTGCCTCCA 
CAAGCCATGA ACAGTGGGTC TCCAGCACCT CAGTCTAATC AGGTGCCCGT GTCTTTACCA 
GTAACTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( NM_001376517.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363446.1 Homo sapiens mediator complex subunit 23 (MED23), transcript variant 5, mRNA.

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CloneID	OHu41025
Clone ID Related Accession (Same CDS sequence)	XM_005267223.3 , NM_001376517.1 , XM_005267223.2
Accession Version	NM_001376517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121575.24. On Nov 16, 2019 this sequence version replaced XM_005267223.3. Transcript Variant: This variant (5) represents the longest transcript and encodes the longest isoform (e). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.179957.1, SRR1803615.266105.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGACCT TGAACATTGC TCAGCACAAG 
CAGCGCTGCC CTGTGCTGGA GGACCAGTTG GTGGATCTGG TTGTTTATGC CATGGAGCGA 
TCTGAGACCG AGGAGAAGTT TGACGATGGG GGAACAAGCC AACTCCTGTG GCAGCATCTC 
TCAAGTCAGC TCATTTTCTT TGTGCTTTTC CAGTTTGCAA GTTTTCCACA TATGGTGCTT 
TCTCTTCATC AGAAGTTAGC AGGGCGAGGA CTGATTAAAG GCAGAGATCA TCTTATGTGG 
GTTCTCCTGC AATTCATTTC TGGAAGTATT CAGAAAAATG CACTAGCTGA TTTTCTCCCT 
GTGATGAAGC TCTTCGACTT GCTATACCCA GAAAAAGAAT ATATCCCAGT TCCTGATATT 
AACAAACCCC AGTCAACCCA TGCCTTTGCA ATGACCTGTA TTTGGATTCA TCTCAATAGA 
AAAGCTCAAA ATGACAACTC CAAGCTACAG ATTCCAATAC CTCATTCCCT AAGACTTCAC 
CATGAGTTCC TGCAGCAGAG TCTAAGAAAT AAAAGTTTAC AGATGAATGA CTATAAGATT 
GCTCTATTGT GTAATGCATA CTCTACAAAT TCAGAATGTT TTACATTACC CATGGGAGCT 
CTGGTAGAAA CTATTTATGG AAATGGAATT ATGAGGATAC CTCTCCCTGG AACAAACTGT 
ATGGCTTCAG GATCTATTAC CCCCTTACCT ATGAACCTCC TGGATTCACT GACAGTTCAT 
GCCAAAATGA GCCTTATTCA CAGCATTGCA ACCAGGGTGA TAAAACTTGC TCATGCAAAG 
TCCAGTGTGG CCTTGGCTCC AGCCCTAGTG GAAACTTACA GTCGTTTATT GGTCTATATG 
GAAATAGAGT CTTTGGGCAT CAAAGGATTT ATCAGTCAGC TTTTGCCAAC TGTTTTCAAA 
TCACATGCAT GGGGGATCTT ACACACACTC CTTGAGATGT TTAGCTACCG GATGCATCAT 
ATTCAGCCTC ATTACAGAGT TCAGCTCCTG AGTCATCTTC ATACTTTGGC TGCAGTTGCA 
CAAACAAACC AGAACCAGCT CCATCTTTGT GTCGAGAGCA CTGCTCTCAG GCTTATAACA 
GCATTAGGTA GCTCAGAGGT ACAACCGCAG TTTACACGCT TCCTTAGTGA TCCCAAAACA 
GTGCTCTCAG CAGAATCTGA AGAACTGAAC CGAGCCTTGA TATTGACCTT GGCTAGAGCA 
ACTCATGTAA CAGATTTTTT TACAGGCTCT GATTCAATTC AGGGAACTTG GTGTAAAGAC 
ATACTTCAGA CCATCATGAG TTTCACTCCT CATAATTGGG CTTCACACAC CCTGAGCTGT 
TTTCCAGGCC CACTACAGGC ATTCTTCAAA CAAAATAATG TGCCTCAGGA AAGCCGTTTT 
AATCTGAAAA AAAATGTGGA GGAGGAGTAT AGGAAGTGGA AGTCAATGAG CAACGAAAAC 
GACATTATTA CCCACTTCTC TATGCAGGGC TCCCCTCCTC TCTTTCTTTG TCTTCTCTGG 
AAAATGCTCT TGGAAACAGA TCATATTAAT CAGATTGGCT ATAGAGTATT AGAGAGAATT 
GGAGCCAGGG CCTTGGTAGC CCATGTGAGG ACATTTGCAG ATTTCCTGGT ATATGAGTTT 
TCTACATCAG CAGGGGGTCA GCAACTCAAT AAATGCATTG AAATTCTTAA TGACATGGTA 
TGGAAGTATA ACATTGTTAC ACTGGACAGA TTAATTCTCT GCCTGGCCAT GCGTAGTCAC 
GAAGGAAATG AAGCCCAGGT TTGTTATTTC ATAATTCAGT TGCTGTTACT CAAACCAAAC 
GATTTTAGAA ATCGAGTAAG TGACTTTGTG AAGGAAAATT CCCCAGAGCA CTGGTTACAG 
AATGACTGGC ACACCAAGCA CATGAATTAT CACAAGAAAT ATCCAGAGAA GTTGTATTTT 
GAGGGCCTCG CGGAACAGGT GGATCCTCCT GTACAGATCC AGTCTCCCTA TCTGCCCATC 
TATTTTGGGA ATGTGTGTCT TCGATTCCTT CCAGTATTTG ATATAGTAAT CCACAGATTT 
TTAGAGTTGC TTCCGGTATC CAAATCACTG GAGACTCTAC TGGATCATCT AGGAGGCTTA 
TATAAATTTC ATGATCGTCC AGTGACTTAT CTGTATAACA CTCTGCACTA TTATGAAATG 
CACCTGAGAG ACCGCGCATT TCTCAAACGA AAACTCGTCC ATGCGATCAT TGGCTCTCTG 
AAGGATAATC GACCGCAGGG CTGGTGTCTA AGTGACACTT ACCTGAAATG CGCTATGAAT 
GCACGAGAGG AAAATCCTTG GGTTCCAGAT GACACCTACT ATTGCAGATT GATTGGCAGA 
CTAGTCGATA CGATGGCTGG CAAATCTCCT GGTCCCTTTC CAAACTGTGA CTGGAGATTC 
AATGAGTTTC CCAACCCAGC TGCCCATGCT CTCCATGTTA CTTGTGTGGA GCTCATGGCC 
TTGGCAGTTT CAGGCAAAGA AGTTGGGAAT GCCCTTCTAA ATGTTGTCCT AAAAAGTCAG 
CCTTTAGTGC CAAGAGAGAA CATTACAGCA TGGATGAATG CAATTGGTTT GATCATCACT 
GCCCTACCAG AGCCATATTG GATTGTTCTT CATGATCGAA TTGTGAGTGT CATCAGCAGC 
CCCAGCTTGA CGTCTGAAAC AGAGTGGGTT GGCTATCCAT TCCGCCTCTT TGATTTCACT 
GCCTGTCATC AGTCCTACTC TGAGATGAGT TGTAGCTATA CGTTAGCTCT TGCACATGCT 
GTGTGGCACC ATTCTAGCAT CGGACAACTT TCTCTCATTC CAAAGTTTCT TACTGAAGTA 
CTTCTTCCTA TAGTGAAGAC CGAATTCCAG TTGCTTTATG TATACCATCT TGTTGGACCA 
TTTTTACAAA GATTTCAGCA AGAGAGAACT CGTTGTATGA TAGAGATTGG TGTGGCGTTT 
TATGACATGC TGCTGAATGT TGACCAGTGT AGCACCCATT TAAATTACAT GGATCCCATC 
TGTGACTTCC TCTATCACAT GAAGTATATG TTTACTGGTG ACAGCGTGAA AGAGCAAGTA 
GAGAAGATTA TCTGTAACTT AAAACCAGCT TTAAAACTTC GTCTTCGATT CATCACACAC 
ATTAGCAAGA TGGAGCCAGC TGCAGTGCCT CCACAAGCCA TGAACAGTGG GTCTCCAGCA 
CCTCAGTCTA ATCAGGTGCC CGTGTCTTTA CCAGTAACTC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363446.1
CDS	194..4318
Translation	METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQTLNIAQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQVPVSLPVTQ

Target ORF information:

RefSeq Version	NM_001376517.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376517.1

ORF Insert Sequence:

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGACCT TGAACATTGC TCAGCACAAG 
CAGCGCTGCC CTGTGCTGGA GGACCAGTTG GTGGATCTGG TTGTTTATGC CATGGAGCGA 
TCTGAGACCG AGGAGAAGTT TGACGATGGG GGAACAAGCC AACTCCTGTG GCAGCATCTC 
TCAAGTCAGC TCATTTTCTT TGTGCTTTTC CAGTTTGCAA GTTTTCCACA TATGGTGCTT 
TCTCTTCATC AGAAGTTAGC AGGGCGAGGA CTGATTAAAG GCAGAGATCA TCTTATGTGG 
GTTCTCCTGC AATTCATTTC TGGAAGTATT CAGAAAAATG CACTAGCTGA TTTTCTCCCT 
GTGATGAAGC TCTTCGACTT GCTATACCCA GAAAAAGAAT ATATCCCAGT TCCTGATATT 
AACAAACCCC AGTCAACCCA TGCCTTTGCA ATGACCTGTA TTTGGATTCA TCTCAATAGA 
AAAGCTCAAA ATGACAACTC CAAGCTACAG ATTCCAATAC CTCATTCCCT AAGACTTCAC 
CATGAGTTCC TGCAGCAGAG TCTAAGAAAT AAAAGTTTAC AGATGAATGA CTATAAGATT 
GCTCTATTGT GTAATGCATA CTCTACAAAT TCAGAATGTT TTACATTACC CATGGGAGCT 
CTGGTAGAAA CTATTTATGG AAATGGAATT ATGAGGATAC CTCTCCCTGG AACAAACTGT 
ATGGCTTCAG GATCTATTAC CCCCTTACCT ATGAACCTCC TGGATTCACT GACAGTTCAT 
GCCAAAATGA GCCTTATTCA CAGCATTGCA ACCAGGGTGA TAAAACTTGC TCATGCAAAG 
TCCAGTGTGG CCTTGGCTCC AGCCCTAGTG GAAACTTACA GTCGTTTATT GGTCTATATG 
GAAATAGAGT CTTTGGGCAT CAAAGGATTT ATCAGTCAGC TTTTGCCAAC TGTTTTCAAA 
TCACATGCAT GGGGGATCTT ACACACACTC CTTGAGATGT TTAGCTACCG GATGCATCAT 
ATTCAGCCTC ATTACAGAGT TCAGCTCCTG AGTCATCTTC ATACTTTGGC TGCAGTTGCA 
CAAACAAACC AGAACCAGCT CCATCTTTGT GTCGAGAGCA CTGCTCTCAG GCTTATAACA 
GCATTAGGTA GCTCAGAGGT ACAACCGCAG TTTACACGCT TCCTTAGTGA TCCCAAAACA 
GTGCTCTCAG CAGAATCTGA AGAACTGAAC CGAGCCTTGA TATTGACCTT GGCTAGAGCA 
ACTCATGTAA CAGATTTTTT TACAGGCTCT GATTCAATTC AGGGAACTTG GTGTAAAGAC 
ATACTTCAGA CCATCATGAG TTTCACTCCT CATAATTGGG CTTCACACAC CCTGAGCTGT 
TTTCCAGGCC CACTACAGGC ATTCTTCAAA CAAAATAATG TGCCTCAGGA AAGCCGTTTT 
AATCTGAAAA AAAATGTGGA GGAGGAGTAT AGGAAGTGGA AGTCAATGAG CAACGAAAAC 
GACATTATTA CCCACTTCTC TATGCAGGGC TCCCCTCCTC TCTTTCTTTG TCTTCTCTGG 
AAAATGCTCT TGGAAACAGA TCATATTAAT CAGATTGGCT ATAGAGTATT AGAGAGAATT 
GGAGCCAGGG CCTTGGTAGC CCATGTGAGG ACATTTGCAG ATTTCCTGGT ATATGAGTTT 
TCTACATCAG CAGGGGGTCA GCAACTCAAT AAATGCATTG AAATTCTTAA TGACATGGTA 
TGGAAGTATA ACATTGTTAC ACTGGACAGA TTAATTCTCT GCCTGGCCAT GCGTAGTCAC 
GAAGGAAATG AAGCCCAGGT TTGTTATTTC ATAATTCAGT TGCTGTTACT CAAACCAAAC 
GATTTTAGAA ATCGAGTAAG TGACTTTGTG AAGGAAAATT CCCCAGAGCA CTGGTTACAG 
AATGACTGGC ACACCAAGCA CATGAATTAT CACAAGAAAT ATCCAGAGAA GTTGTATTTT 
GAGGGCCTCG CGGAACAGGT GGATCCTCCT GTACAGATCC AGTCTCCCTA TCTGCCCATC 
TATTTTGGGA ATGTGTGTCT TCGATTCCTT CCAGTATTTG ATATAGTAAT CCACAGATTT 
TTAGAGTTGC TTCCGGTATC CAAATCACTG GAGACTCTAC TGGATCATCT AGGAGGCTTA 
TATAAATTTC ATGATCGTCC AGTGACTTAT CTGTATAACA CTCTGCACTA TTATGAAATG 
CACCTGAGAG ACCGCGCATT TCTCAAACGA AAACTCGTCC ATGCGATCAT TGGCTCTCTG 
AAGGATAATC GACCGCAGGG CTGGTGTCTA AGTGACACTT ACCTGAAATG CGCTATGAAT 
GCACGAGAGG AAAATCCTTG GGTTCCAGAT GACACCTACT ATTGCAGATT GATTGGCAGA 
CTAGTCGATA CGATGGCTGG CAAATCTCCT GGTCCCTTTC CAAACTGTGA CTGGAGATTC 
AATGAGTTTC CCAACCCAGC TGCCCATGCT CTCCATGTTA CTTGTGTGGA GCTCATGGCC 
TTGGCAGTTT CAGGCAAAGA AGTTGGGAAT GCCCTTCTAA ATGTTGTCCT AAAAAGTCAG 
CCTTTAGTGC CAAGAGAGAA CATTACAGCA TGGATGAATG CAATTGGTTT GATCATCACT 
GCCCTACCAG AGCCATATTG GATTGTTCTT CATGATCGAA TTGTGAGTGT CATCAGCAGC 
CCCAGCTTGA CGTCTGAAAC AGAGTGGGTT GGCTATCCAT TCCGCCTCTT TGATTTCACT 
GCCTGTCATC AGTCCTACTC TGAGATGAGT TGTAGCTATA CGTTAGCTCT TGCACATGCT 
GTGTGGCACC ATTCTAGCAT CGGACAACTT TCTCTCATTC CAAAGTTTCT TACTGAAGTA 
CTTCTTCCTA TAGTGAAGAC CGAATTCCAG TTGCTTTATG TATACCATCT TGTTGGACCA 
TTTTTACAAA GATTTCAGCA AGAGAGAACT CGTTGTATGA TAGAGATTGG TGTGGCGTTT 
TATGACATGC TGCTGAATGT TGACCAGTGT AGCACCCATT TAAATTACAT GGATCCCATC 
TGTGACTTCC TCTATCACAT GAAGTATATG TTTACTGGTG ACAGCGTGAA AGAGCAAGTA 
GAGAAGATTA TCTGTAACTT AAAACCAGCT TTAAAACTTC GTCTTCGATT CATCACACAC 
ATTAGCAAGA TGGAGCCAGC TGCAGTGCCT CCACAAGCCA TGAACAGTGG GTCTCCAGCA 
CCTCAGTCTA ATCAGGTGCC CGTGTCTTTA CCAGTAACTC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( NM_001270521.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257450.1 Homo sapiens mediator complex subunit 23 (MED23), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu03689
Clone ID Related Accession (Same CDS sequence)	NM_001270521.1 , NM_001270521.2
Accession Version	NM_001270521.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB453121.1, BC007088.1, BC060759.1 and AW295188.1. Transcript Variant: This variant (3) uses an alternate splice junction in the 3' end compared to variant 1. The resulting isoform (c) has a shorter and distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC060759.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG 
GAGGACCAGT TGGTGGATCT GGTTGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG 
TTTGACGATG GGGGAACAAG CCAACTCCTG TGGCAGCATC TCTCAAGTCA GCTCATTTTC 
TTTGTGCTTT TCCAGTTTGC AAGTTTTCCA CATATGGTGC TTTCTCTTCA TCAGAAGTTA 
GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCCT GCAATTCATT 
TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTCC CTGTGATGAA GCTCTTCGAC 
TTGCTATACC CAGAAAAAGA ATATATCCCA GTTCCTGATA TTAACAAACC CCAGTCAACC 
CATGCCTTTG CAATGACCTG TATTTGGATT CATCTCAATA GAAAAGCTCA AAATGACAAC 
TCCAAGCTAC AGATTCCAAT ACCTCATTCC CTAAGACTTC ACCATGAGTT CCTGCAGCAG 
AGTCTAAGAA ATAAAAGTTT ACAGATGAAT GACTATAAGA TTGCTCTATT GTGTAATGCA 
TACTCTACAA ATTCAGAATG TTTTACATTA CCCATGGGAG CTCTGGTAGA AACTATTTAT 
GGAAATGGAA TTATGAGGAT ACCTCTCCCT GGAACAAACT GTATGGCTTC AGGATCTATT 
ACCCCCTTAC CTATGAACCT CCTGGATTCA CTGACAGTTC ATGCCAAAAT GAGCCTTATT 
CACAGCATTG CAACCAGGGT GATAAAACTT GCTCATGCAA AGTCCAGTGT GGCCTTGGCT 
CCAGCCCTAG TGGAAACTTA CAGTCGTTTA TTGGTCTATA TGGAAATAGA GTCTTTGGGC 
ATCAAAGGAT TTATCAGTCA GCTTTTGCCA ACTGTTTTCA AATCACATGC ATGGGGGATC 
TTACACACAC TCCTTGAGAT GTTTAGCTAC CGGATGCATC ATATTCAGCC TCATTACAGA 
GTTCAGCTCC TGAGTCATCT TCATACTTTG GCTGCAGTTG CACAAACAAA CCAGAACCAG 
CTCCATCTTT GTGTCGAGAG CACTGCTCTC AGGCTTATAA CAGCATTAGG TAGCTCAGAG 
GTACAACCGC AGTTTACACG CTTCCTTAGT GATCCCAAAA CAGTGCTCTC AGCAGAATCT 
GAAGAACTGA ACCGAGCCTT GATATTGACC TTGGCTAGAG CAACTCATGT AACAGATTTT 
TTTACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG 
AGTTTCACTC CTCATAATTG GGCTTCACAC ACCCTGAGCT GTTTTCCAGG CCCACTACAG 
GCATTCTTCA AACAAAATAA TGTGCCTCAG GAAAGCCGTT TTAATCTGAA AAAAAATGTG 
GAGGAGGAGT ATAGGAAGTG GAAGTCAATG AGCAACGAAA ACGACATTAT TACCCACTTC 
TCTATGCAGG GCTCCCCTCC TCTCTTTCTT TGTCTTCTCT GGAAAATGCT CTTGGAAACA 
GATCATATTA ATCAGATTGG CTATAGAGTA TTAGAGAGAA TTGGAGCCAG GGCCTTGGTA 
GCCCATGTGA GGACATTTGC AGATTTCCTG GTATATGAGT TTTCTACATC AGCAGGGGGT 
CAGCAACTCA ATAAATGCAT TGAAATTCTT AATGACATGG TATGGAAGTA TAACATTGTT 
ACACTGGACA GATTAATTCT CTGCCTGGCC ATGCGTAGTC ACGAAGGAAA TGAAGCCCAG 
GTTTGTTATT TCATAATTCA GTTGCTGTTA CTCAAACCAA ACGATTTTAG AAATCGAGTA 
AGTGACTTTG TGAAGGAAAA TTCCCCAGAG CACTGGTTAC AGAATGACTG GCACACCAAG 
CACATGAATT ATCACAAGAA ATATCCAGAG AAGTTGTATT TTGAGGGCCT CGCGGAACAG 
GTGGATCCTC CTGTACAGAT CCAGTCTCCC TATCTGCCCA TCTATTTTGG GAATGTGTGT 
CTTCGATTCC TTCCAGTATT TGATATAGTA ATCCACAGAT TTTTAGAGTT GCTTCCGGTA 
TCCAAATCAC TGGAGACTCT ACTGGATCAT CTAGGAGGCT TATATAAATT TCATGATCGT 
CCAGTGACTT ATCTGTATAA CACTCTGCAC TATTATGAAA TGCACCTGAG AGACCGCGCA 
TTTCTCAAAC GAAAACTCGT CCATGCGATC ATTGGCTCTC TGAAGGATAA TCGACCGCAG 
GGCTGGTGTC TAAGTGACAC TTACCTGAAA TGCGCTATGA ATGCACGAGA GGAAAATCCT 
TGGGTTCCAG ATGACACCTA CTATTGCAGA TTGATTGGCA GACTAGTCGA TACGATGGCT 
GGCAAATCTC CTGGTCCCTT TCCAAACTGT GACTGGAGAT TCAATGAGTT TCCCAACCCA 
GCTGCCCATG CTCTCCATGT TACTTGTGTG GAGCTCATGG CCTTGGCAGT TTCAGGCAAA 
GAAGTTGGGA ATGCCCTTCT AAATGTTGTC CTAAAAAGTC AGCCTTTAGT GCCAAGAGAG 
AACATTACAG CATGGATGAA TGCAATTGGT TTGATCATCA CTGCCCTACC AGAGCCATAT 
TGGATTGTTC TTCATGATCG AATTGTGAGT GTCATCAGCA GCCCCAGCTT GACGTCTGAA 
ACAGAGTGGG TTGGCTATCC ATTCCGCCTC TTTGATTTCA CTGCCTGTCA TCAGTCCTAC 
TCTGAGATGA GTTGTAGCTA TACGTTAGCT CTTGCACATG CTGTGTGGCA CCATTCTAGC 
ATCGGACAAC TTTCTCTCAT TCCAAAGTTT CTTACTGAAG TACTTCTTCC TATAGTGAAG 
ACCGAATTCC AGTTGCTTTA TGTATACCAT CTTGTTGGAC CATTTTTACA AAGATTTCAG 
CAAGAGAGAA CTCGTTGTAT GATAGAGATT GGTGTGGCGT TTTATGACAT GCTGCTGAAT 
GTTGACCAGT GTAGCACCCA TTTAAATTAC ATGGATCCCA TCTGTGACTT CCTCTATCAC 
ATGAAGTATA TGTTTACTGG TGACAGCGTG AAAGAGCAAG TAGAGAAGAT TATCTGTAAC 
TTAAAACCAG CTTTAAAACT TCGTCTTCGA TTCATCACAC ACATTAGCAA GATGGAGCCA 
GCTGCAGTGC CTCCACAAGC CATGAACAGT GGGTCTCCAG CACCTCAGTC TAATCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257450.1
CDS	191..4270
Translation	METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQ

Target ORF information:

RefSeq Version	NM_001270521.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270521.1

ORF Insert Sequence:

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG 
GAGGACCAGT TGGTGGATCT GGTTGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG 
TTTGACGATG GGGGAACAAG CCAACTCCTG TGGCAGCATC TCTCAAGTCA GCTCATTTTC 
TTTGTGCTTT TCCAGTTTGC AAGTTTTCCA CATATGGTGC TTTCTCTTCA TCAGAAGTTA 
GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCCT GCAATTCATT 
TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTCC CTGTGATGAA GCTCTTCGAC 
TTGCTATACC CAGAAAAAGA ATATATCCCA GTTCCTGATA TTAACAAACC CCAGTCAACC 
CATGCCTTTG CAATGACCTG TATTTGGATT CATCTCAATA GAAAAGCTCA AAATGACAAC 
TCCAAGCTAC AGATTCCAAT ACCTCATTCC CTAAGACTTC ACCATGAGTT CCTGCAGCAG 
AGTCTAAGAA ATAAAAGTTT ACAGATGAAT GACTATAAGA TTGCTCTATT GTGTAATGCA 
TACTCTACAA ATTCAGAATG TTTTACATTA CCCATGGGAG CTCTGGTAGA AACTATTTAT 
GGAAATGGAA TTATGAGGAT ACCTCTCCCT GGAACAAACT GTATGGCTTC AGGATCTATT 
ACCCCCTTAC CTATGAACCT CCTGGATTCA CTGACAGTTC ATGCCAAAAT GAGCCTTATT 
CACAGCATTG CAACCAGGGT GATAAAACTT GCTCATGCAA AGTCCAGTGT GGCCTTGGCT 
CCAGCCCTAG TGGAAACTTA CAGTCGTTTA TTGGTCTATA TGGAAATAGA GTCTTTGGGC 
ATCAAAGGAT TTATCAGTCA GCTTTTGCCA ACTGTTTTCA AATCACATGC ATGGGGGATC 
TTACACACAC TCCTTGAGAT GTTTAGCTAC CGGATGCATC ATATTCAGCC TCATTACAGA 
GTTCAGCTCC TGAGTCATCT TCATACTTTG GCTGCAGTTG CACAAACAAA CCAGAACCAG 
CTCCATCTTT GTGTCGAGAG CACTGCTCTC AGGCTTATAA CAGCATTAGG TAGCTCAGAG 
GTACAACCGC AGTTTACACG CTTCCTTAGT GATCCCAAAA CAGTGCTCTC AGCAGAATCT 
GAAGAACTGA ACCGAGCCTT GATATTGACC TTGGCTAGAG CAACTCATGT AACAGATTTT 
TTTACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG 
AGTTTCACTC CTCATAATTG GGCTTCACAC ACCCTGAGCT GTTTTCCAGG CCCACTACAG 
GCATTCTTCA AACAAAATAA TGTGCCTCAG GAAAGCCGTT TTAATCTGAA AAAAAATGTG 
GAGGAGGAGT ATAGGAAGTG GAAGTCAATG AGCAACGAAA ACGACATTAT TACCCACTTC 
TCTATGCAGG GCTCCCCTCC TCTCTTTCTT TGTCTTCTCT GGAAAATGCT CTTGGAAACA 
GATCATATTA ATCAGATTGG CTATAGAGTA TTAGAGAGAA TTGGAGCCAG GGCCTTGGTA 
GCCCATGTGA GGACATTTGC AGATTTCCTG GTATATGAGT TTTCTACATC AGCAGGGGGT 
CAGCAACTCA ATAAATGCAT TGAAATTCTT AATGACATGG TATGGAAGTA TAACATTGTT 
ACACTGGACA GATTAATTCT CTGCCTGGCC ATGCGTAGTC ACGAAGGAAA TGAAGCCCAG 
GTTTGTTATT TCATAATTCA GTTGCTGTTA CTCAAACCAA ACGATTTTAG AAATCGAGTA 
AGTGACTTTG TGAAGGAAAA TTCCCCAGAG CACTGGTTAC AGAATGACTG GCACACCAAG 
CACATGAATT ATCACAAGAA ATATCCAGAG AAGTTGTATT TTGAGGGCCT CGCGGAACAG 
GTGGATCCTC CTGTACAGAT CCAGTCTCCC TATCTGCCCA TCTATTTTGG GAATGTGTGT 
CTTCGATTCC TTCCAGTATT TGATATAGTA ATCCACAGAT TTTTAGAGTT GCTTCCGGTA 
TCCAAATCAC TGGAGACTCT ACTGGATCAT CTAGGAGGCT TATATAAATT TCATGATCGT 
CCAGTGACTT ATCTGTATAA CACTCTGCAC TATTATGAAA TGCACCTGAG AGACCGCGCA 
TTTCTCAAAC GAAAACTCGT CCATGCGATC ATTGGCTCTC TGAAGGATAA TCGACCGCAG 
GGCTGGTGTC TAAGTGACAC TTACCTGAAA TGCGCTATGA ATGCACGAGA GGAAAATCCT 
TGGGTTCCAG ATGACACCTA CTATTGCAGA TTGATTGGCA GACTAGTCGA TACGATGGCT 
GGCAAATCTC CTGGTCCCTT TCCAAACTGT GACTGGAGAT TCAATGAGTT TCCCAACCCA 
GCTGCCCATG CTCTCCATGT TACTTGTGTG GAGCTCATGG CCTTGGCAGT TTCAGGCAAA 
GAAGTTGGGA ATGCCCTTCT AAATGTTGTC CTAAAAAGTC AGCCTTTAGT GCCAAGAGAG 
AACATTACAG CATGGATGAA TGCAATTGGT TTGATCATCA CTGCCCTACC AGAGCCATAT 
TGGATTGTTC TTCATGATCG AATTGTGAGT GTCATCAGCA GCCCCAGCTT GACGTCTGAA 
ACAGAGTGGG TTGGCTATCC ATTCCGCCTC TTTGATTTCA CTGCCTGTCA TCAGTCCTAC 
TCTGAGATGA GTTGTAGCTA TACGTTAGCT CTTGCACATG CTGTGTGGCA CCATTCTAGC 
ATCGGACAAC TTTCTCTCAT TCCAAAGTTT CTTACTGAAG TACTTCTTCC TATAGTGAAG 
ACCGAATTCC AGTTGCTTTA TGTATACCAT CTTGTTGGAC CATTTTTACA AAGATTTCAG 
CAAGAGAGAA CTCGTTGTAT GATAGAGATT GGTGTGGCGT TTTATGACAT GCTGCTGAAT 
GTTGACCAGT GTAGCACCCA TTTAAATTAC ATGGATCCCA TCTGTGACTT CCTCTATCAC 
ATGAAGTATA TGTTTACTGG TGACAGCGTG AAAGAGCAAG TAGAGAAGAT TATCTGTAAC 
TTAAAACCAG CTTTAAAACT TCGTCTTCGA TTCATCACAC ACATTAGCAA GATGGAGCCA 
GCTGCAGTGC CTCCACAAGC CATGAACAGT GGGTCTCCAG CACCTCAGTC TAATCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( NM_001270521.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257450.1 Homo sapiens mediator complex subunit 23 (MED23), transcript variant 3, mRNA.

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CloneID	OHu03689
Clone ID Related Accession (Same CDS sequence)	NM_001270521.1 , NM_001270521.2
Accession Version	NM_001270521.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121575.24, DB453121.1, BC007088.1, BC060759.1 and AW295188.1. On May 31, 2019 this sequence version replaced NM_001270521.1. Transcript Variant: This variant (3) uses an alternate splice junction in the 3' end compared to variant 1. The resulting isoform (c) has a shorter and distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC060759.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG 
GAGGACCAGT TGGTGGATCT GGTTGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG 
TTTGACGATG GGGGAACAAG CCAACTCCTG TGGCAGCATC TCTCAAGTCA GCTCATTTTC 
TTTGTGCTTT TCCAGTTTGC AAGTTTTCCA CATATGGTGC TTTCTCTTCA TCAGAAGTTA 
GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCCT GCAATTCATT 
TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTCC CTGTGATGAA GCTCTTCGAC 
TTGCTATACC CAGAAAAAGA ATATATCCCA GTTCCTGATA TTAACAAACC CCAGTCAACC 
CATGCCTTTG CAATGACCTG TATTTGGATT CATCTCAATA GAAAAGCTCA AAATGACAAC 
TCCAAGCTAC AGATTCCAAT ACCTCATTCC CTAAGACTTC ACCATGAGTT CCTGCAGCAG 
AGTCTAAGAA ATAAAAGTTT ACAGATGAAT GACTATAAGA TTGCTCTATT GTGTAATGCA 
TACTCTACAA ATTCAGAATG TTTTACATTA CCCATGGGAG CTCTGGTAGA AACTATTTAT 
GGAAATGGAA TTATGAGGAT ACCTCTCCCT GGAACAAACT GTATGGCTTC AGGATCTATT 
ACCCCCTTAC CTATGAACCT CCTGGATTCA CTGACAGTTC ATGCCAAAAT GAGCCTTATT 
CACAGCATTG CAACCAGGGT GATAAAACTT GCTCATGCAA AGTCCAGTGT GGCCTTGGCT 
CCAGCCCTAG TGGAAACTTA CAGTCGTTTA TTGGTCTATA TGGAAATAGA GTCTTTGGGC 
ATCAAAGGAT TTATCAGTCA GCTTTTGCCA ACTGTTTTCA AATCACATGC ATGGGGGATC 
TTACACACAC TCCTTGAGAT GTTTAGCTAC CGGATGCATC ATATTCAGCC TCATTACAGA 
GTTCAGCTCC TGAGTCATCT TCATACTTTG GCTGCAGTTG CACAAACAAA CCAGAACCAG 
CTCCATCTTT GTGTCGAGAG CACTGCTCTC AGGCTTATAA CAGCATTAGG TAGCTCAGAG 
GTACAACCGC AGTTTACACG CTTCCTTAGT GATCCCAAAA CAGTGCTCTC AGCAGAATCT 
GAAGAACTGA ACCGAGCCTT GATATTGACC TTGGCTAGAG CAACTCATGT AACAGATTTT 
TTTACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG 
AGTTTCACTC CTCATAATTG GGCTTCACAC ACCCTGAGCT GTTTTCCAGG CCCACTACAG 
GCATTCTTCA AACAAAATAA TGTGCCTCAG GAAAGCCGTT TTAATCTGAA AAAAAATGTG 
GAGGAGGAGT ATAGGAAGTG GAAGTCAATG AGCAACGAAA ACGACATTAT TACCCACTTC 
TCTATGCAGG GCTCCCCTCC TCTCTTTCTT TGTCTTCTCT GGAAAATGCT CTTGGAAACA 
GATCATATTA ATCAGATTGG CTATAGAGTA TTAGAGAGAA TTGGAGCCAG GGCCTTGGTA 
GCCCATGTGA GGACATTTGC AGATTTCCTG GTATATGAGT TTTCTACATC AGCAGGGGGT 
CAGCAACTCA ATAAATGCAT TGAAATTCTT AATGACATGG TATGGAAGTA TAACATTGTT 
ACACTGGACA GATTAATTCT CTGCCTGGCC ATGCGTAGTC ACGAAGGAAA TGAAGCCCAG 
GTTTGTTATT TCATAATTCA GTTGCTGTTA CTCAAACCAA ACGATTTTAG AAATCGAGTA 
AGTGACTTTG TGAAGGAAAA TTCCCCAGAG CACTGGTTAC AGAATGACTG GCACACCAAG 
CACATGAATT ATCACAAGAA ATATCCAGAG AAGTTGTATT TTGAGGGCCT CGCGGAACAG 
GTGGATCCTC CTGTACAGAT CCAGTCTCCC TATCTGCCCA TCTATTTTGG GAATGTGTGT 
CTTCGATTCC TTCCAGTATT TGATATAGTA ATCCACAGAT TTTTAGAGTT GCTTCCGGTA 
TCCAAATCAC TGGAGACTCT ACTGGATCAT CTAGGAGGCT TATATAAATT TCATGATCGT 
CCAGTGACTT ATCTGTATAA CACTCTGCAC TATTATGAAA TGCACCTGAG AGACCGCGCA 
TTTCTCAAAC GAAAACTCGT CCATGCGATC ATTGGCTCTC TGAAGGATAA TCGACCGCAG 
GGCTGGTGTC TAAGTGACAC TTACCTGAAA TGCGCTATGA ATGCACGAGA GGAAAATCCT 
TGGGTTCCAG ATGACACCTA CTATTGCAGA TTGATTGGCA GACTAGTCGA TACGATGGCT 
GGCAAATCTC CTGGTCCCTT TCCAAACTGT GACTGGAGAT TCAATGAGTT TCCCAACCCA 
GCTGCCCATG CTCTCCATGT TACTTGTGTG GAGCTCATGG CCTTGGCAGT TTCAGGCAAA 
GAAGTTGGGA ATGCCCTTCT AAATGTTGTC CTAAAAAGTC AGCCTTTAGT GCCAAGAGAG 
AACATTACAG CATGGATGAA TGCAATTGGT TTGATCATCA CTGCCCTACC AGAGCCATAT 
TGGATTGTTC TTCATGATCG AATTGTGAGT GTCATCAGCA GCCCCAGCTT GACGTCTGAA 
ACAGAGTGGG TTGGCTATCC ATTCCGCCTC TTTGATTTCA CTGCCTGTCA TCAGTCCTAC 
TCTGAGATGA GTTGTAGCTA TACGTTAGCT CTTGCACATG CTGTGTGGCA CCATTCTAGC 
ATCGGACAAC TTTCTCTCAT TCCAAAGTTT CTTACTGAAG TACTTCTTCC TATAGTGAAG 
ACCGAATTCC AGTTGCTTTA TGTATACCAT CTTGTTGGAC CATTTTTACA AAGATTTCAG 
CAAGAGAGAA CTCGTTGTAT GATAGAGATT GGTGTGGCGT TTTATGACAT GCTGCTGAAT 
GTTGACCAGT GTAGCACCCA TTTAAATTAC ATGGATCCCA TCTGTGACTT CCTCTATCAC 
ATGAAGTATA TGTTTACTGG TGACAGCGTG AAAGAGCAAG TAGAGAAGAT TATCTGTAAC 
TTAAAACCAG CTTTAAAACT TCGTCTTCGA TTCATCACAC ACATTAGCAA GATGGAGCCA 
GCTGCAGTGC CTCCACAAGC CATGAACAGT GGGTCTCCAG CACCTCAGTC TAATCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257450.1
CDS	194..4273
Translation	METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQ

Target ORF information:

RefSeq Version	NM_001270521.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270521.2

ORF Insert Sequence:

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG 
GAGGACCAGT TGGTGGATCT GGTTGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG 
TTTGACGATG GGGGAACAAG CCAACTCCTG TGGCAGCATC TCTCAAGTCA GCTCATTTTC 
TTTGTGCTTT TCCAGTTTGC AAGTTTTCCA CATATGGTGC TTTCTCTTCA TCAGAAGTTA 
GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCCT GCAATTCATT 
TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTCC CTGTGATGAA GCTCTTCGAC 
TTGCTATACC CAGAAAAAGA ATATATCCCA GTTCCTGATA TTAACAAACC CCAGTCAACC 
CATGCCTTTG CAATGACCTG TATTTGGATT CATCTCAATA GAAAAGCTCA AAATGACAAC 
TCCAAGCTAC AGATTCCAAT ACCTCATTCC CTAAGACTTC ACCATGAGTT CCTGCAGCAG 
AGTCTAAGAA ATAAAAGTTT ACAGATGAAT GACTATAAGA TTGCTCTATT GTGTAATGCA 
TACTCTACAA ATTCAGAATG TTTTACATTA CCCATGGGAG CTCTGGTAGA AACTATTTAT 
GGAAATGGAA TTATGAGGAT ACCTCTCCCT GGAACAAACT GTATGGCTTC AGGATCTATT 
ACCCCCTTAC CTATGAACCT CCTGGATTCA CTGACAGTTC ATGCCAAAAT GAGCCTTATT 
CACAGCATTG CAACCAGGGT GATAAAACTT GCTCATGCAA AGTCCAGTGT GGCCTTGGCT 
CCAGCCCTAG TGGAAACTTA CAGTCGTTTA TTGGTCTATA TGGAAATAGA GTCTTTGGGC 
ATCAAAGGAT TTATCAGTCA GCTTTTGCCA ACTGTTTTCA AATCACATGC ATGGGGGATC 
TTACACACAC TCCTTGAGAT GTTTAGCTAC CGGATGCATC ATATTCAGCC TCATTACAGA 
GTTCAGCTCC TGAGTCATCT TCATACTTTG GCTGCAGTTG CACAAACAAA CCAGAACCAG 
CTCCATCTTT GTGTCGAGAG CACTGCTCTC AGGCTTATAA CAGCATTAGG TAGCTCAGAG 
GTACAACCGC AGTTTACACG CTTCCTTAGT GATCCCAAAA CAGTGCTCTC AGCAGAATCT 
GAAGAACTGA ACCGAGCCTT GATATTGACC TTGGCTAGAG CAACTCATGT AACAGATTTT 
TTTACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG 
AGTTTCACTC CTCATAATTG GGCTTCACAC ACCCTGAGCT GTTTTCCAGG CCCACTACAG 
GCATTCTTCA AACAAAATAA TGTGCCTCAG GAAAGCCGTT TTAATCTGAA AAAAAATGTG 
GAGGAGGAGT ATAGGAAGTG GAAGTCAATG AGCAACGAAA ACGACATTAT TACCCACTTC 
TCTATGCAGG GCTCCCCTCC TCTCTTTCTT TGTCTTCTCT GGAAAATGCT CTTGGAAACA 
GATCATATTA ATCAGATTGG CTATAGAGTA TTAGAGAGAA TTGGAGCCAG GGCCTTGGTA 
GCCCATGTGA GGACATTTGC AGATTTCCTG GTATATGAGT TTTCTACATC AGCAGGGGGT 
CAGCAACTCA ATAAATGCAT TGAAATTCTT AATGACATGG TATGGAAGTA TAACATTGTT 
ACACTGGACA GATTAATTCT CTGCCTGGCC ATGCGTAGTC ACGAAGGAAA TGAAGCCCAG 
GTTTGTTATT TCATAATTCA GTTGCTGTTA CTCAAACCAA ACGATTTTAG AAATCGAGTA 
AGTGACTTTG TGAAGGAAAA TTCCCCAGAG CACTGGTTAC AGAATGACTG GCACACCAAG 
CACATGAATT ATCACAAGAA ATATCCAGAG AAGTTGTATT TTGAGGGCCT CGCGGAACAG 
GTGGATCCTC CTGTACAGAT CCAGTCTCCC TATCTGCCCA TCTATTTTGG GAATGTGTGT 
CTTCGATTCC TTCCAGTATT TGATATAGTA ATCCACAGAT TTTTAGAGTT GCTTCCGGTA 
TCCAAATCAC TGGAGACTCT ACTGGATCAT CTAGGAGGCT TATATAAATT TCATGATCGT 
CCAGTGACTT ATCTGTATAA CACTCTGCAC TATTATGAAA TGCACCTGAG AGACCGCGCA 
TTTCTCAAAC GAAAACTCGT CCATGCGATC ATTGGCTCTC TGAAGGATAA TCGACCGCAG 
GGCTGGTGTC TAAGTGACAC TTACCTGAAA TGCGCTATGA ATGCACGAGA GGAAAATCCT 
TGGGTTCCAG ATGACACCTA CTATTGCAGA TTGATTGGCA GACTAGTCGA TACGATGGCT 
GGCAAATCTC CTGGTCCCTT TCCAAACTGT GACTGGAGAT TCAATGAGTT TCCCAACCCA 
GCTGCCCATG CTCTCCATGT TACTTGTGTG GAGCTCATGG CCTTGGCAGT TTCAGGCAAA 
GAAGTTGGGA ATGCCCTTCT AAATGTTGTC CTAAAAAGTC AGCCTTTAGT GCCAAGAGAG 
AACATTACAG CATGGATGAA TGCAATTGGT TTGATCATCA CTGCCCTACC AGAGCCATAT 
TGGATTGTTC TTCATGATCG AATTGTGAGT GTCATCAGCA GCCCCAGCTT GACGTCTGAA 
ACAGAGTGGG TTGGCTATCC ATTCCGCCTC TTTGATTTCA CTGCCTGTCA TCAGTCCTAC 
TCTGAGATGA GTTGTAGCTA TACGTTAGCT CTTGCACATG CTGTGTGGCA CCATTCTAGC 
ATCGGACAAC TTTCTCTCAT TCCAAAGTTT CTTACTGAAG TACTTCTTCC TATAGTGAAG 
ACCGAATTCC AGTTGCTTTA TGTATACCAT CTTGTTGGAC CATTTTTACA AAGATTTCAG 
CAAGAGAGAA CTCGTTGTAT GATAGAGATT GGTGTGGCGT TTTATGACAT GCTGCTGAAT 
GTTGACCAGT GTAGCACCCA TTTAAATTAC ATGGATCCCA TCTGTGACTT CCTCTATCAC 
ATGAAGTATA TGTTTACTGG TGACAGCGTG AAAGAGCAAG TAGAGAAGAT TATCTGTAAC 
TTAAAACCAG CTTTAAAACT TCGTCTTCGA TTCATCACAC ACATTAGCAA GATGGAGCCA 
GCTGCAGTGC CTCCACAAGC CATGAACAGT GGGTCTCCAG CACCTCAGTC TAATCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( NM_001270522.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257451.1 Homo sapiens mediator complex subunit 23 (MED23), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu03690
Clone ID Related Accession (Same CDS sequence)	NM_001270522.1 , NM_001270522.2
Accession Version	NM_001270522.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB453121.1, AB033042.1, AF135022.1 and AI249380.1. Transcript Variant: This variant (4) uses an alternate splice junction in the 3' end compared to variant 1. The resulting isoform (d) has a shorter and distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF135022.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG 
GAGGACCAGT TGGTGGATCT GGTTGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG 
TTTGACGATG GGGGAACAAG CCAACTCCTG TGGCAGCATC TCTCAAGTCA GCTCATTTTC 
TTTGTGCTTT TCCAGTTTGC AAGTTTTCCA CATATGGTGC TTTCTCTTCA TCAGAAGTTA 
GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCCT GCAATTCATT 
TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTCC CTGTGATGAA GCTCTTCGAC 
TTGCTATACC CAGAAAAAGA ATATATCCCA GTTCCTGATA TTAACAAACC CCAGTCAACC 
CATGCCTTTG CAATGACCTG TATTTGGATT CATCTCAATA GAAAAGCTCA AAATGACAAC 
TCCAAGCTAC AGATTCCAAT ACCTCATTCC CTAAGACTTC ACCATGAGTT CCTGCAGCAG 
AGTCTAAGAA ATAAAAGTTT ACAGATGAAT GACTATAAGA TTGCTCTATT GTGTAATGCA 
TACTCTACAA ATTCAGAATG TTTTACATTA CCCATGGGAG CTCTGGTAGA AACTATTTAT 
GGAAATGGAA TTATGAGGAT ACCTCTCCCT GGAACAAACT GTATGGCTTC AGGATCTATT 
ACCCCCTTAC CTATGAACCT CCTGGATTCA CTGACAGTTC ATGCCAAAAT GAGCCTTATT 
CACAGCATTG CAACCAGGGT GATAAAACTT GCTCATGCAA AGTCCAGTGT GGCCTTGGCT 
CCAGCCCTAG TGGAAACTTA CAGTCGTTTA TTGGTCTATA TGGAAATAGA GTCTTTGGGC 
ATCAAAGGAT TTATCAGTCA GCTTTTGCCA ACTGTTTTCA AATCACATGC ATGGGGGATC 
TTACACACAC TCCTTGAGAT GTTTAGCTAC CGGATGCATC ATATTCAGCC TCATTACAGA 
GTTCAGCTCC TGAGTCATCT TCATACTTTG GCTGCAGTTG CACAAACAAA CCAGAACCAG 
CTCCATCTTT GTGTCGAGAG CACTGCTCTC AGGCTTATAA CAGCATTAGG TAGCTCAGAG 
GTACAACCGC AGTTTACACG CTTCCTTAGT GATCCCAAAA CAGTGCTCTC AGCAGAATCT 
GAAGAACTGA ACCGAGCCTT GATATTGACC TTGGCTAGAG CAACTCATGT AACAGATTTT 
TTTACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG 
AGTTTCACTC CTCATAATTG GGCTTCACAC ACCCTGAGCT GTTTTCCAGG CCCACTACAG 
GCATTCTTCA AACAAAATAA TGTGCCTCAG GAAAGCCGTT TTAATCTGAA AAAAAATGTG 
GAGGAGGAGT ATAGGAAGTG GAAGTCAATG AGCAACGAAA ACGACATTAT TACCCACTTC 
TCTATGCAGG GCTCCCCTCC TCTCTTTCTT TGTCTTCTCT GGAAAATGCT CTTGGAAACA 
GATCATATTA ATCAGATTGG CTATAGAGTA TTAGAGAGAA TTGGAGCCAG GGCCTTGGTA 
GCCCATGTGA GGACATTTGC AGATTTCCTG GTATATGAGT TTTCTACATC AGCAGGGGGT 
CAGCAACTCA ATAAATGCAT TGAAATTCTT AATGACATGG TATGGAAGTA TAACATTGTT 
ACACTGGACA GATTAATTCT CTGCCTGGCC ATGCGTAGTC ACGAAGGAAA TGAAGCCCAG 
GTTTGTTATT TCATAATTCA GTTGCTGTTA CTCAAACCAA ACGATTTTAG AAATCGAGTA 
AGTGACTTTG TGAAGGAAAA TTCCCCAGAG CACTGGTTAC AGAATGACTG GCACACCAAG 
CACATGAATT ATCACAAGAA ATATCCAGAG AAGTTGTATT TTGAGGGCCT CGCGGAACAG 
GTGGATCCTC CTGTACAGAT CCAGTCTCCC TATCTGCCCA TCTATTTTGG GAATGTGTGT 
CTTCGATTCC TTCCAGTATT TGATATAGTA ATCCACAGAT TTTTAGAGTT GCTTCCGGTA 
TCCAAATCAC TGGAGACTCT ACTGGATCAT CTAGGAGGCT TATATAAATT TCATGATCGT 
CCAGTGACTT ATCTGTATAA CACTCTGCAC TATTATGAAA TGCACCTGAG AGACCGCGCA 
TTTCTCAAAC GAAAACTCGT CCATGCGATC ATTGGCTCTC TGAAGGATAA TCGACCGCAG 
GGCTGGTGTC TAAGTGACAC TTACCTGAAA TGCGCTATGA ATGCACGAGA GGAAAATCCT 
TGGGTTCCAG ATGACACCTA CTATTGCAGA TTGATTGGCA GACTAGTCGA TACGATGGCT 
GGCAAATCTC CTGGTCCCTT TCCAAACTGT GACTGGAGAT TCAATGAGTT TCCCAACCCA 
GCTGCCCATG CTCTCCATGT TACTTGTGTG GAGCTCATGG CCTTGGCAGT TTCAGGCAAA 
GAAGTTGGGA ATGCCCTTCT AAATGTTGTC CTAAAAAGTC AGCCTTTAGT GCCAAGAGAG 
AACATTACAG CATGGATGAA TGCAATTGGT TTGATCATCA CTGCCCTACC AGAGCCATAT 
TGGATTGTTC TTCATGATCG AATTGTGAGT GTCATCAGCA GCCCCAGCTT GACGTCTGAA 
ACAGAGTGGG TTGGCTATCC ATTCCGCCTC TTTGATTTCA CTGCCTGTCA TCAGTCCTAC 
TCTGAGATGA GTTGTAGCTA TACGTTAGCT CTTGCACATG CTGTGTGGCA CCATTCTAGC 
ATCGGACAAC TTTCTCTCAT TCCAAAGTTT CTTACTGAAG TACTTCTTCC TATAGTGAAG 
ACCGAATTCC AGTTGCTTTA TGTATACCAT CTTGTTGGAC CATTTTTACA AAGATTTCAG 
CAAGAGAGAA CTCGTTGTAT GATAGAGATT GGTGTGGCGT TTTATGACAT GCTGCTGAAT 
GTTGACCAGT GTAGCACCCA TTTAAATTAC ATGGATCCCA TCTGTGACTT CCTCTATCAC 
ATGAAGTATA TGTTTACTGG TGACAGCGTG AAAGAGCAAG TAGAGAAGAT TATCTGTAAC 
TTAAAACCAG CTTTAAAACT TCGTCTTCGA TTCATCACAC ACATTAGCAA GATGGAGCCA 
GCTGCAGTGC CTCCACAAGC CATGAACAGT GGGTCTCCAG CACCTCAGTC TAATCAGGTT 
GACACTCTCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257451.1
CDS	191..4285
Translation	METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQVDTLT

Target ORF information:

RefSeq Version	NM_001270522.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270522.1

ORF Insert Sequence:

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG 
GAGGACCAGT TGGTGGATCT GGTTGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG 
TTTGACGATG GGGGAACAAG CCAACTCCTG TGGCAGCATC TCTCAAGTCA GCTCATTTTC 
TTTGTGCTTT TCCAGTTTGC AAGTTTTCCA CATATGGTGC TTTCTCTTCA TCAGAAGTTA 
GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCCT GCAATTCATT 
TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTCC CTGTGATGAA GCTCTTCGAC 
TTGCTATACC CAGAAAAAGA ATATATCCCA GTTCCTGATA TTAACAAACC CCAGTCAACC 
CATGCCTTTG CAATGACCTG TATTTGGATT CATCTCAATA GAAAAGCTCA AAATGACAAC 
TCCAAGCTAC AGATTCCAAT ACCTCATTCC CTAAGACTTC ACCATGAGTT CCTGCAGCAG 
AGTCTAAGAA ATAAAAGTTT ACAGATGAAT GACTATAAGA TTGCTCTATT GTGTAATGCA 
TACTCTACAA ATTCAGAATG TTTTACATTA CCCATGGGAG CTCTGGTAGA AACTATTTAT 
GGAAATGGAA TTATGAGGAT ACCTCTCCCT GGAACAAACT GTATGGCTTC AGGATCTATT 
ACCCCCTTAC CTATGAACCT CCTGGATTCA CTGACAGTTC ATGCCAAAAT GAGCCTTATT 
CACAGCATTG CAACCAGGGT GATAAAACTT GCTCATGCAA AGTCCAGTGT GGCCTTGGCT 
CCAGCCCTAG TGGAAACTTA CAGTCGTTTA TTGGTCTATA TGGAAATAGA GTCTTTGGGC 
ATCAAAGGAT TTATCAGTCA GCTTTTGCCA ACTGTTTTCA AATCACATGC ATGGGGGATC 
TTACACACAC TCCTTGAGAT GTTTAGCTAC CGGATGCATC ATATTCAGCC TCATTACAGA 
GTTCAGCTCC TGAGTCATCT TCATACTTTG GCTGCAGTTG CACAAACAAA CCAGAACCAG 
CTCCATCTTT GTGTCGAGAG CACTGCTCTC AGGCTTATAA CAGCATTAGG TAGCTCAGAG 
GTACAACCGC AGTTTACACG CTTCCTTAGT GATCCCAAAA CAGTGCTCTC AGCAGAATCT 
GAAGAACTGA ACCGAGCCTT GATATTGACC TTGGCTAGAG CAACTCATGT AACAGATTTT 
TTTACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG 
AGTTTCACTC CTCATAATTG GGCTTCACAC ACCCTGAGCT GTTTTCCAGG CCCACTACAG 
GCATTCTTCA AACAAAATAA TGTGCCTCAG GAAAGCCGTT TTAATCTGAA AAAAAATGTG 
GAGGAGGAGT ATAGGAAGTG GAAGTCAATG AGCAACGAAA ACGACATTAT TACCCACTTC 
TCTATGCAGG GCTCCCCTCC TCTCTTTCTT TGTCTTCTCT GGAAAATGCT CTTGGAAACA 
GATCATATTA ATCAGATTGG CTATAGAGTA TTAGAGAGAA TTGGAGCCAG GGCCTTGGTA 
GCCCATGTGA GGACATTTGC AGATTTCCTG GTATATGAGT TTTCTACATC AGCAGGGGGT 
CAGCAACTCA ATAAATGCAT TGAAATTCTT AATGACATGG TATGGAAGTA TAACATTGTT 
ACACTGGACA GATTAATTCT CTGCCTGGCC ATGCGTAGTC ACGAAGGAAA TGAAGCCCAG 
GTTTGTTATT TCATAATTCA GTTGCTGTTA CTCAAACCAA ACGATTTTAG AAATCGAGTA 
AGTGACTTTG TGAAGGAAAA TTCCCCAGAG CACTGGTTAC AGAATGACTG GCACACCAAG 
CACATGAATT ATCACAAGAA ATATCCAGAG AAGTTGTATT TTGAGGGCCT CGCGGAACAG 
GTGGATCCTC CTGTACAGAT CCAGTCTCCC TATCTGCCCA TCTATTTTGG GAATGTGTGT 
CTTCGATTCC TTCCAGTATT TGATATAGTA ATCCACAGAT TTTTAGAGTT GCTTCCGGTA 
TCCAAATCAC TGGAGACTCT ACTGGATCAT CTAGGAGGCT TATATAAATT TCATGATCGT 
CCAGTGACTT ATCTGTATAA CACTCTGCAC TATTATGAAA TGCACCTGAG AGACCGCGCA 
TTTCTCAAAC GAAAACTCGT CCATGCGATC ATTGGCTCTC TGAAGGATAA TCGACCGCAG 
GGCTGGTGTC TAAGTGACAC TTACCTGAAA TGCGCTATGA ATGCACGAGA GGAAAATCCT 
TGGGTTCCAG ATGACACCTA CTATTGCAGA TTGATTGGCA GACTAGTCGA TACGATGGCT 
GGCAAATCTC CTGGTCCCTT TCCAAACTGT GACTGGAGAT TCAATGAGTT TCCCAACCCA 
GCTGCCCATG CTCTCCATGT TACTTGTGTG GAGCTCATGG CCTTGGCAGT TTCAGGCAAA 
GAAGTTGGGA ATGCCCTTCT AAATGTTGTC CTAAAAAGTC AGCCTTTAGT GCCAAGAGAG 
AACATTACAG CATGGATGAA TGCAATTGGT TTGATCATCA CTGCCCTACC AGAGCCATAT 
TGGATTGTTC TTCATGATCG AATTGTGAGT GTCATCAGCA GCCCCAGCTT GACGTCTGAA 
ACAGAGTGGG TTGGCTATCC ATTCCGCCTC TTTGATTTCA CTGCCTGTCA TCAGTCCTAC 
TCTGAGATGA GTTGTAGCTA TACGTTAGCT CTTGCACATG CTGTGTGGCA CCATTCTAGC 
ATCGGACAAC TTTCTCTCAT TCCAAAGTTT CTTACTGAAG TACTTCTTCC TATAGTGAAG 
ACCGAATTCC AGTTGCTTTA TGTATACCAT CTTGTTGGAC CATTTTTACA AAGATTTCAG 
CAAGAGAGAA CTCGTTGTAT GATAGAGATT GGTGTGGCGT TTTATGACAT GCTGCTGAAT 
GTTGACCAGT GTAGCACCCA TTTAAATTAC ATGGATCCCA TCTGTGACTT CCTCTATCAC 
ATGAAGTATA TGTTTACTGG TGACAGCGTG AAAGAGCAAG TAGAGAAGAT TATCTGTAAC 
TTAAAACCAG CTTTAAAACT TCGTCTTCGA TTCATCACAC ACATTAGCAA GATGGAGCCA 
GCTGCAGTGC CTCCACAAGC CATGAACAGT GGGTCTCCAG CACCTCAGTC TAATCAGGTT 
GACACTCTCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( NM_001270522.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257451.1 Homo sapiens mediator complex subunit 23 (MED23), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu03690
Clone ID Related Accession (Same CDS sequence)	NM_001270522.1 , NM_001270522.2
Accession Version	NM_001270522.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121575.24, DB453121.1, AB033042.1, AF135022.1 and AI249380.1. On May 31, 2019 this sequence version replaced NM_001270522.1. Transcript Variant: This variant (4) uses an alternate splice junction in the 3' end compared to variant 1. The resulting isoform (d) has a shorter and distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF135022.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG 
GAGGACCAGT TGGTGGATCT GGTTGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG 
TTTGACGATG GGGGAACAAG CCAACTCCTG TGGCAGCATC TCTCAAGTCA GCTCATTTTC 
TTTGTGCTTT TCCAGTTTGC AAGTTTTCCA CATATGGTGC TTTCTCTTCA TCAGAAGTTA 
GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCCT GCAATTCATT 
TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTCC CTGTGATGAA GCTCTTCGAC 
TTGCTATACC CAGAAAAAGA ATATATCCCA GTTCCTGATA TTAACAAACC CCAGTCAACC 
CATGCCTTTG CAATGACCTG TATTTGGATT CATCTCAATA GAAAAGCTCA AAATGACAAC 
TCCAAGCTAC AGATTCCAAT ACCTCATTCC CTAAGACTTC ACCATGAGTT CCTGCAGCAG 
AGTCTAAGAA ATAAAAGTTT ACAGATGAAT GACTATAAGA TTGCTCTATT GTGTAATGCA 
TACTCTACAA ATTCAGAATG TTTTACATTA CCCATGGGAG CTCTGGTAGA AACTATTTAT 
GGAAATGGAA TTATGAGGAT ACCTCTCCCT GGAACAAACT GTATGGCTTC AGGATCTATT 
ACCCCCTTAC CTATGAACCT CCTGGATTCA CTGACAGTTC ATGCCAAAAT GAGCCTTATT 
CACAGCATTG CAACCAGGGT GATAAAACTT GCTCATGCAA AGTCCAGTGT GGCCTTGGCT 
CCAGCCCTAG TGGAAACTTA CAGTCGTTTA TTGGTCTATA TGGAAATAGA GTCTTTGGGC 
ATCAAAGGAT TTATCAGTCA GCTTTTGCCA ACTGTTTTCA AATCACATGC ATGGGGGATC 
TTACACACAC TCCTTGAGAT GTTTAGCTAC CGGATGCATC ATATTCAGCC TCATTACAGA 
GTTCAGCTCC TGAGTCATCT TCATACTTTG GCTGCAGTTG CACAAACAAA CCAGAACCAG 
CTCCATCTTT GTGTCGAGAG CACTGCTCTC AGGCTTATAA CAGCATTAGG TAGCTCAGAG 
GTACAACCGC AGTTTACACG CTTCCTTAGT GATCCCAAAA CAGTGCTCTC AGCAGAATCT 
GAAGAACTGA ACCGAGCCTT GATATTGACC TTGGCTAGAG CAACTCATGT AACAGATTTT 
TTTACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG 
AGTTTCACTC CTCATAATTG GGCTTCACAC ACCCTGAGCT GTTTTCCAGG CCCACTACAG 
GCATTCTTCA AACAAAATAA TGTGCCTCAG GAAAGCCGTT TTAATCTGAA AAAAAATGTG 
GAGGAGGAGT ATAGGAAGTG GAAGTCAATG AGCAACGAAA ACGACATTAT TACCCACTTC 
TCTATGCAGG GCTCCCCTCC TCTCTTTCTT TGTCTTCTCT GGAAAATGCT CTTGGAAACA 
GATCATATTA ATCAGATTGG CTATAGAGTA TTAGAGAGAA TTGGAGCCAG GGCCTTGGTA 
GCCCATGTGA GGACATTTGC AGATTTCCTG GTATATGAGT TTTCTACATC AGCAGGGGGT 
CAGCAACTCA ATAAATGCAT TGAAATTCTT AATGACATGG TATGGAAGTA TAACATTGTT 
ACACTGGACA GATTAATTCT CTGCCTGGCC ATGCGTAGTC ACGAAGGAAA TGAAGCCCAG 
GTTTGTTATT TCATAATTCA GTTGCTGTTA CTCAAACCAA ACGATTTTAG AAATCGAGTA 
AGTGACTTTG TGAAGGAAAA TTCCCCAGAG CACTGGTTAC AGAATGACTG GCACACCAAG 
CACATGAATT ATCACAAGAA ATATCCAGAG AAGTTGTATT TTGAGGGCCT CGCGGAACAG 
GTGGATCCTC CTGTACAGAT CCAGTCTCCC TATCTGCCCA TCTATTTTGG GAATGTGTGT 
CTTCGATTCC TTCCAGTATT TGATATAGTA ATCCACAGAT TTTTAGAGTT GCTTCCGGTA 
TCCAAATCAC TGGAGACTCT ACTGGATCAT CTAGGAGGCT TATATAAATT TCATGATCGT 
CCAGTGACTT ATCTGTATAA CACTCTGCAC TATTATGAAA TGCACCTGAG AGACCGCGCA 
TTTCTCAAAC GAAAACTCGT CCATGCGATC ATTGGCTCTC TGAAGGATAA TCGACCGCAG 
GGCTGGTGTC TAAGTGACAC TTACCTGAAA TGCGCTATGA ATGCACGAGA GGAAAATCCT 
TGGGTTCCAG ATGACACCTA CTATTGCAGA TTGATTGGCA GACTAGTCGA TACGATGGCT 
GGCAAATCTC CTGGTCCCTT TCCAAACTGT GACTGGAGAT TCAATGAGTT TCCCAACCCA 
GCTGCCCATG CTCTCCATGT TACTTGTGTG GAGCTCATGG CCTTGGCAGT TTCAGGCAAA 
GAAGTTGGGA ATGCCCTTCT AAATGTTGTC CTAAAAAGTC AGCCTTTAGT GCCAAGAGAG 
AACATTACAG CATGGATGAA TGCAATTGGT TTGATCATCA CTGCCCTACC AGAGCCATAT 
TGGATTGTTC TTCATGATCG AATTGTGAGT GTCATCAGCA GCCCCAGCTT GACGTCTGAA 
ACAGAGTGGG TTGGCTATCC ATTCCGCCTC TTTGATTTCA CTGCCTGTCA TCAGTCCTAC 
TCTGAGATGA GTTGTAGCTA TACGTTAGCT CTTGCACATG CTGTGTGGCA CCATTCTAGC 
ATCGGACAAC TTTCTCTCAT TCCAAAGTTT CTTACTGAAG TACTTCTTCC TATAGTGAAG 
ACCGAATTCC AGTTGCTTTA TGTATACCAT CTTGTTGGAC CATTTTTACA AAGATTTCAG 
CAAGAGAGAA CTCGTTGTAT GATAGAGATT GGTGTGGCGT TTTATGACAT GCTGCTGAAT 
GTTGACCAGT GTAGCACCCA TTTAAATTAC ATGGATCCCA TCTGTGACTT CCTCTATCAC 
ATGAAGTATA TGTTTACTGG TGACAGCGTG AAAGAGCAAG TAGAGAAGAT TATCTGTAAC 
TTAAAACCAG CTTTAAAACT TCGTCTTCGA TTCATCACAC ACATTAGCAA GATGGAGCCA 
GCTGCAGTGC CTCCACAAGC CATGAACAGT GGGTCTCCAG CACCTCAGTC TAATCAGGTT 
GACACTCTCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257451.1
CDS	194..4288
Translation	METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQVDTLT

Target ORF information:

RefSeq Version	NM_001270522.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270522.2

ORF Insert Sequence:

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGCACA AGCAGCGCTG CCCTGTGCTG 
GAGGACCAGT TGGTGGATCT GGTTGTTTAT GCCATGGAGC GATCTGAGAC CGAGGAGAAG 
TTTGACGATG GGGGAACAAG CCAACTCCTG TGGCAGCATC TCTCAAGTCA GCTCATTTTC 
TTTGTGCTTT TCCAGTTTGC AAGTTTTCCA CATATGGTGC TTTCTCTTCA TCAGAAGTTA 
GCAGGGCGAG GACTGATTAA AGGCAGAGAT CATCTTATGT GGGTTCTCCT GCAATTCATT 
TCTGGAAGTA TTCAGAAAAA TGCACTAGCT GATTTTCTCC CTGTGATGAA GCTCTTCGAC 
TTGCTATACC CAGAAAAAGA ATATATCCCA GTTCCTGATA TTAACAAACC CCAGTCAACC 
CATGCCTTTG CAATGACCTG TATTTGGATT CATCTCAATA GAAAAGCTCA AAATGACAAC 
TCCAAGCTAC AGATTCCAAT ACCTCATTCC CTAAGACTTC ACCATGAGTT CCTGCAGCAG 
AGTCTAAGAA ATAAAAGTTT ACAGATGAAT GACTATAAGA TTGCTCTATT GTGTAATGCA 
TACTCTACAA ATTCAGAATG TTTTACATTA CCCATGGGAG CTCTGGTAGA AACTATTTAT 
GGAAATGGAA TTATGAGGAT ACCTCTCCCT GGAACAAACT GTATGGCTTC AGGATCTATT 
ACCCCCTTAC CTATGAACCT CCTGGATTCA CTGACAGTTC ATGCCAAAAT GAGCCTTATT 
CACAGCATTG CAACCAGGGT GATAAAACTT GCTCATGCAA AGTCCAGTGT GGCCTTGGCT 
CCAGCCCTAG TGGAAACTTA CAGTCGTTTA TTGGTCTATA TGGAAATAGA GTCTTTGGGC 
ATCAAAGGAT TTATCAGTCA GCTTTTGCCA ACTGTTTTCA AATCACATGC ATGGGGGATC 
TTACACACAC TCCTTGAGAT GTTTAGCTAC CGGATGCATC ATATTCAGCC TCATTACAGA 
GTTCAGCTCC TGAGTCATCT TCATACTTTG GCTGCAGTTG CACAAACAAA CCAGAACCAG 
CTCCATCTTT GTGTCGAGAG CACTGCTCTC AGGCTTATAA CAGCATTAGG TAGCTCAGAG 
GTACAACCGC AGTTTACACG CTTCCTTAGT GATCCCAAAA CAGTGCTCTC AGCAGAATCT 
GAAGAACTGA ACCGAGCCTT GATATTGACC TTGGCTAGAG CAACTCATGT AACAGATTTT 
TTTACAGGCT CTGATTCAAT TCAGGGAACT TGGTGTAAAG ACATACTTCA GACCATCATG 
AGTTTCACTC CTCATAATTG GGCTTCACAC ACCCTGAGCT GTTTTCCAGG CCCACTACAG 
GCATTCTTCA AACAAAATAA TGTGCCTCAG GAAAGCCGTT TTAATCTGAA AAAAAATGTG 
GAGGAGGAGT ATAGGAAGTG GAAGTCAATG AGCAACGAAA ACGACATTAT TACCCACTTC 
TCTATGCAGG GCTCCCCTCC TCTCTTTCTT TGTCTTCTCT GGAAAATGCT CTTGGAAACA 
GATCATATTA ATCAGATTGG CTATAGAGTA TTAGAGAGAA TTGGAGCCAG GGCCTTGGTA 
GCCCATGTGA GGACATTTGC AGATTTCCTG GTATATGAGT TTTCTACATC AGCAGGGGGT 
CAGCAACTCA ATAAATGCAT TGAAATTCTT AATGACATGG TATGGAAGTA TAACATTGTT 
ACACTGGACA GATTAATTCT CTGCCTGGCC ATGCGTAGTC ACGAAGGAAA TGAAGCCCAG 
GTTTGTTATT TCATAATTCA GTTGCTGTTA CTCAAACCAA ACGATTTTAG AAATCGAGTA 
AGTGACTTTG TGAAGGAAAA TTCCCCAGAG CACTGGTTAC AGAATGACTG GCACACCAAG 
CACATGAATT ATCACAAGAA ATATCCAGAG AAGTTGTATT TTGAGGGCCT CGCGGAACAG 
GTGGATCCTC CTGTACAGAT CCAGTCTCCC TATCTGCCCA TCTATTTTGG GAATGTGTGT 
CTTCGATTCC TTCCAGTATT TGATATAGTA ATCCACAGAT TTTTAGAGTT GCTTCCGGTA 
TCCAAATCAC TGGAGACTCT ACTGGATCAT CTAGGAGGCT TATATAAATT TCATGATCGT 
CCAGTGACTT ATCTGTATAA CACTCTGCAC TATTATGAAA TGCACCTGAG AGACCGCGCA 
TTTCTCAAAC GAAAACTCGT CCATGCGATC ATTGGCTCTC TGAAGGATAA TCGACCGCAG 
GGCTGGTGTC TAAGTGACAC TTACCTGAAA TGCGCTATGA ATGCACGAGA GGAAAATCCT 
TGGGTTCCAG ATGACACCTA CTATTGCAGA TTGATTGGCA GACTAGTCGA TACGATGGCT 
GGCAAATCTC CTGGTCCCTT TCCAAACTGT GACTGGAGAT TCAATGAGTT TCCCAACCCA 
GCTGCCCATG CTCTCCATGT TACTTGTGTG GAGCTCATGG CCTTGGCAGT TTCAGGCAAA 
GAAGTTGGGA ATGCCCTTCT AAATGTTGTC CTAAAAAGTC AGCCTTTAGT GCCAAGAGAG 
AACATTACAG CATGGATGAA TGCAATTGGT TTGATCATCA CTGCCCTACC AGAGCCATAT 
TGGATTGTTC TTCATGATCG AATTGTGAGT GTCATCAGCA GCCCCAGCTT GACGTCTGAA 
ACAGAGTGGG TTGGCTATCC ATTCCGCCTC TTTGATTTCA CTGCCTGTCA TCAGTCCTAC 
TCTGAGATGA GTTGTAGCTA TACGTTAGCT CTTGCACATG CTGTGTGGCA CCATTCTAGC 
ATCGGACAAC TTTCTCTCAT TCCAAAGTTT CTTACTGAAG TACTTCTTCC TATAGTGAAG 
ACCGAATTCC AGTTGCTTTA TGTATACCAT CTTGTTGGAC CATTTTTACA AAGATTTCAG 
CAAGAGAGAA CTCGTTGTAT GATAGAGATT GGTGTGGCGT TTTATGACAT GCTGCTGAAT 
GTTGACCAGT GTAGCACCCA TTTAAATTAC ATGGATCCCA TCTGTGACTT CCTCTATCAC 
ATGAAGTATA TGTTTACTGG TGACAGCGTG AAAGAGCAAG TAGAGAAGAT TATCTGTAAC 
TTAAAACCAG CTTTAAAACT TCGTCTTCGA TTCATCACAC ACATTAGCAA GATGGAGCCA 
GCTGCAGTGC CTCCACAAGC CATGAACAGT GGGTCTCCAG CACCTCAGTC TAATCAGGTT 
GACACTCTCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( NM_015979.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057063.2 Homo sapiens mediator complex subunit 23 (MED23), transcript variant 2, mRNA.

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CloneID	OHu19949
Clone ID Related Accession (Same CDS sequence)	NM_015979.4 , NM_015979.3
Accession Version	NM_015979.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121575.24, DB453121.1, AL136776.1, BC060759.1 and AW295188.1. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_015979.3. Transcript Variant: This variant (2) contains an alternate in-frame exon and uses an alternate splice junction in the 3' end compared to variant 1. The resulting isoform (b) contains an alternate short internal segment and is shorter at the C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL136776.1, SRR1803613.71954.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGACCT TGAACATTGC TCAGCACAAG 
CAGCGCTGCC CTGTGCTGGA GGACCAGTTG GTGGATCTGG TTGTTTATGC CATGGAGCGA 
TCTGAGACCG AGGAGAAGTT TGACGATGGG GGAACAAGCC AACTCCTGTG GCAGCATCTC 
TCAAGTCAGC TCATTTTCTT TGTGCTTTTC CAGTTTGCAA GTTTTCCACA TATGGTGCTT 
TCTCTTCATC AGAAGTTAGC AGGGCGAGGA CTGATTAAAG GCAGAGATCA TCTTATGTGG 
GTTCTCCTGC AATTCATTTC TGGAAGTATT CAGAAAAATG CACTAGCTGA TTTTCTCCCT 
GTGATGAAGC TCTTCGACTT GCTATACCCA GAAAAAGAAT ATATCCCAGT TCCTGATATT 
AACAAACCCC AGTCAACCCA TGCCTTTGCA ATGACCTGTA TTTGGATTCA TCTCAATAGA 
AAAGCTCAAA ATGACAACTC CAAGCTACAG ATTCCAATAC CTCATTCCCT AAGACTTCAC 
CATGAGTTCC TGCAGCAGAG TCTAAGAAAT AAAAGTTTAC AGATGAATGA CTATAAGATT 
GCTCTATTGT GTAATGCATA CTCTACAAAT TCAGAATGTT TTACATTACC CATGGGAGCT 
CTGGTAGAAA CTATTTATGG AAATGGAATT ATGAGGATAC CTCTCCCTGG AACAAACTGT 
ATGGCTTCAG GATCTATTAC CCCCTTACCT ATGAACCTCC TGGATTCACT GACAGTTCAT 
GCCAAAATGA GCCTTATTCA CAGCATTGCA ACCAGGGTGA TAAAACTTGC TCATGCAAAG 
TCCAGTGTGG CCTTGGCTCC AGCCCTAGTG GAAACTTACA GTCGTTTATT GGTCTATATG 
GAAATAGAGT CTTTGGGCAT CAAAGGATTT ATCAGTCAGC TTTTGCCAAC TGTTTTCAAA 
TCACATGCAT GGGGGATCTT ACACACACTC CTTGAGATGT TTAGCTACCG GATGCATCAT 
ATTCAGCCTC ATTACAGAGT TCAGCTCCTG AGTCATCTTC ATACTTTGGC TGCAGTTGCA 
CAAACAAACC AGAACCAGCT CCATCTTTGT GTCGAGAGCA CTGCTCTCAG GCTTATAACA 
GCATTAGGTA GCTCAGAGGT ACAACCGCAG TTTACACGCT TCCTTAGTGA TCCCAAAACA 
GTGCTCTCAG CAGAATCTGA AGAACTGAAC CGAGCCTTGA TATTGACCTT GGCTAGAGCA 
ACTCATGTAA CAGATTTTTT TACAGGCTCT GATTCAATTC AGGGAACTTG GTGTAAAGAC 
ATACTTCAGA CCATCATGAG TTTCACTCCT CATAATTGGG CTTCACACAC CCTGAGCTGT 
TTTCCAGGCC CACTACAGGC ATTCTTCAAA CAAAATAATG TGCCTCAGGA AAGCCGTTTT 
AATCTGAAAA AAAATGTGGA GGAGGAGTAT AGGAAGTGGA AGTCAATGAG CAACGAAAAC 
GACATTATTA CCCACTTCTC TATGCAGGGC TCCCCTCCTC TCTTTCTTTG TCTTCTCTGG 
AAAATGCTCT TGGAAACAGA TCATATTAAT CAGATTGGCT ATAGAGTATT AGAGAGAATT 
GGAGCCAGGG CCTTGGTAGC CCATGTGAGG ACATTTGCAG ATTTCCTGGT ATATGAGTTT 
TCTACATCAG CAGGGGGTCA GCAACTCAAT AAATGCATTG AAATTCTTAA TGACATGGTA 
TGGAAGTATA ACATTGTTAC ACTGGACAGA TTAATTCTCT GCCTGGCCAT GCGTAGTCAC 
GAAGGAAATG AAGCCCAGGT TTGTTATTTC ATAATTCAGT TGCTGTTACT CAAACCAAAC 
GATTTTAGAA ATCGAGTAAG TGACTTTGTG AAGGAAAATT CCCCAGAGCA CTGGTTACAG 
AATGACTGGC ACACCAAGCA CATGAATTAT CACAAGAAAT ATCCAGAGAA GTTGTATTTT 
GAGGGCCTCG CGGAACAGGT GGATCCTCCT GTACAGATCC AGTCTCCCTA TCTGCCCATC 
TATTTTGGGA ATGTGTGTCT TCGATTCCTT CCAGTATTTG ATATAGTAAT CCACAGATTT 
TTAGAGTTGC TTCCGGTATC CAAATCACTG GAGACTCTAC TGGATCATCT AGGAGGCTTA 
TATAAATTTC ATGATCGTCC AGTGACTTAT CTGTATAACA CTCTGCACTA TTATGAAATG 
CACCTGAGAG ACCGCGCATT TCTCAAACGA AAACTCGTCC ATGCGATCAT TGGCTCTCTG 
AAGGATAATC GACCGCAGGG CTGGTGTCTA AGTGACACTT ACCTGAAATG CGCTATGAAT 
GCACGAGAGG AAAATCCTTG GGTTCCAGAT GACACCTACT ATTGCAGATT GATTGGCAGA 
CTAGTCGATA CGATGGCTGG CAAATCTCCT GGTCCCTTTC CAAACTGTGA CTGGAGATTC 
AATGAGTTTC CCAACCCAGC TGCCCATGCT CTCCATGTTA CTTGTGTGGA GCTCATGGCC 
TTGGCAGTTT CAGGCAAAGA AGTTGGGAAT GCCCTTCTAA ATGTTGTCCT AAAAAGTCAG 
CCTTTAGTGC CAAGAGAGAA CATTACAGCA TGGATGAATG CAATTGGTTT GATCATCACT 
GCCCTACCAG AGCCATATTG GATTGTTCTT CATGATCGAA TTGTGAGTGT CATCAGCAGC 
CCCAGCTTGA CGTCTGAAAC AGAGTGGGTT GGCTATCCAT TCCGCCTCTT TGATTTCACT 
GCCTGTCATC AGTCCTACTC TGAGATGAGT TGTAGCTATA CGTTAGCTCT TGCACATGCT 
GTGTGGCACC ATTCTAGCAT CGGACAACTT TCTCTCATTC CAAAGTTTCT TACTGAAGTA 
CTTCTTCCTA TAGTGAAGAC CGAATTCCAG TTGCTTTATG TATACCATCT TGTTGGACCA 
TTTTTACAAA GATTTCAGCA AGAGAGAACT CGTTGTATGA TAGAGATTGG TGTGGCGTTT 
TATGACATGC TGCTGAATGT TGACCAGTGT AGCACCCATT TAAATTACAT GGATCCCATC 
TGTGACTTCC TCTATCACAT GAAGTATATG TTTACTGGTG ACAGCGTGAA AGAGCAAGTA 
GAGAAGATTA TCTGTAACTT AAAACCAGCT TTAAAACTTC GTCTTCGATT CATCACACAC 
ATTAGCAAGA TGGAGCCAGC TGCAGTGCCT CCACAAGCCA TGAACAGTGG GTCTCCAGCA 
CCTCAGTCTA ATCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057063.2
CDS	194..4291
Translation	METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQTLNIAQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQ

Target ORF information:

RefSeq Version	NM_015979.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015979.4

ORF Insert Sequence:

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGACCT TGAACATTGC TCAGCACAAG 
CAGCGCTGCC CTGTGCTGGA GGACCAGTTG GTGGATCTGG TTGTTTATGC CATGGAGCGA 
TCTGAGACCG AGGAGAAGTT TGACGATGGG GGAACAAGCC AACTCCTGTG GCAGCATCTC 
TCAAGTCAGC TCATTTTCTT TGTGCTTTTC CAGTTTGCAA GTTTTCCACA TATGGTGCTT 
TCTCTTCATC AGAAGTTAGC AGGGCGAGGA CTGATTAAAG GCAGAGATCA TCTTATGTGG 
GTTCTCCTGC AATTCATTTC TGGAAGTATT CAGAAAAATG CACTAGCTGA TTTTCTCCCT 
GTGATGAAGC TCTTCGACTT GCTATACCCA GAAAAAGAAT ATATCCCAGT TCCTGATATT 
AACAAACCCC AGTCAACCCA TGCCTTTGCA ATGACCTGTA TTTGGATTCA TCTCAATAGA 
AAAGCTCAAA ATGACAACTC CAAGCTACAG ATTCCAATAC CTCATTCCCT AAGACTTCAC 
CATGAGTTCC TGCAGCAGAG TCTAAGAAAT AAAAGTTTAC AGATGAATGA CTATAAGATT 
GCTCTATTGT GTAATGCATA CTCTACAAAT TCAGAATGTT TTACATTACC CATGGGAGCT 
CTGGTAGAAA CTATTTATGG AAATGGAATT ATGAGGATAC CTCTCCCTGG AACAAACTGT 
ATGGCTTCAG GATCTATTAC CCCCTTACCT ATGAACCTCC TGGATTCACT GACAGTTCAT 
GCCAAAATGA GCCTTATTCA CAGCATTGCA ACCAGGGTGA TAAAACTTGC TCATGCAAAG 
TCCAGTGTGG CCTTGGCTCC AGCCCTAGTG GAAACTTACA GTCGTTTATT GGTCTATATG 
GAAATAGAGT CTTTGGGCAT CAAAGGATTT ATCAGTCAGC TTTTGCCAAC TGTTTTCAAA 
TCACATGCAT GGGGGATCTT ACACACACTC CTTGAGATGT TTAGCTACCG GATGCATCAT 
ATTCAGCCTC ATTACAGAGT TCAGCTCCTG AGTCATCTTC ATACTTTGGC TGCAGTTGCA 
CAAACAAACC AGAACCAGCT CCATCTTTGT GTCGAGAGCA CTGCTCTCAG GCTTATAACA 
GCATTAGGTA GCTCAGAGGT ACAACCGCAG TTTACACGCT TCCTTAGTGA TCCCAAAACA 
GTGCTCTCAG CAGAATCTGA AGAACTGAAC CGAGCCTTGA TATTGACCTT GGCTAGAGCA 
ACTCATGTAA CAGATTTTTT TACAGGCTCT GATTCAATTC AGGGAACTTG GTGTAAAGAC 
ATACTTCAGA CCATCATGAG TTTCACTCCT CATAATTGGG CTTCACACAC CCTGAGCTGT 
TTTCCAGGCC CACTACAGGC ATTCTTCAAA CAAAATAATG TGCCTCAGGA AAGCCGTTTT 
AATCTGAAAA AAAATGTGGA GGAGGAGTAT AGGAAGTGGA AGTCAATGAG CAACGAAAAC 
GACATTATTA CCCACTTCTC TATGCAGGGC TCCCCTCCTC TCTTTCTTTG TCTTCTCTGG 
AAAATGCTCT TGGAAACAGA TCATATTAAT CAGATTGGCT ATAGAGTATT AGAGAGAATT 
GGAGCCAGGG CCTTGGTAGC CCATGTGAGG ACATTTGCAG ATTTCCTGGT ATATGAGTTT 
TCTACATCAG CAGGGGGTCA GCAACTCAAT AAATGCATTG AAATTCTTAA TGACATGGTA 
TGGAAGTATA ACATTGTTAC ACTGGACAGA TTAATTCTCT GCCTGGCCAT GCGTAGTCAC 
GAAGGAAATG AAGCCCAGGT TTGTTATTTC ATAATTCAGT TGCTGTTACT CAAACCAAAC 
GATTTTAGAA ATCGAGTAAG TGACTTTGTG AAGGAAAATT CCCCAGAGCA CTGGTTACAG 
AATGACTGGC ACACCAAGCA CATGAATTAT CACAAGAAAT ATCCAGAGAA GTTGTATTTT 
GAGGGCCTCG CGGAACAGGT GGATCCTCCT GTACAGATCC AGTCTCCCTA TCTGCCCATC 
TATTTTGGGA ATGTGTGTCT TCGATTCCTT CCAGTATTTG ATATAGTAAT CCACAGATTT 
TTAGAGTTGC TTCCGGTATC CAAATCACTG GAGACTCTAC TGGATCATCT AGGAGGCTTA 
TATAAATTTC ATGATCGTCC AGTGACTTAT CTGTATAACA CTCTGCACTA TTATGAAATG 
CACCTGAGAG ACCGCGCATT TCTCAAACGA AAACTCGTCC ATGCGATCAT TGGCTCTCTG 
AAGGATAATC GACCGCAGGG CTGGTGTCTA AGTGACACTT ACCTGAAATG CGCTATGAAT 
GCACGAGAGG AAAATCCTTG GGTTCCAGAT GACACCTACT ATTGCAGATT GATTGGCAGA 
CTAGTCGATA CGATGGCTGG CAAATCTCCT GGTCCCTTTC CAAACTGTGA CTGGAGATTC 
AATGAGTTTC CCAACCCAGC TGCCCATGCT CTCCATGTTA CTTGTGTGGA GCTCATGGCC 
TTGGCAGTTT CAGGCAAAGA AGTTGGGAAT GCCCTTCTAA ATGTTGTCCT AAAAAGTCAG 
CCTTTAGTGC CAAGAGAGAA CATTACAGCA TGGATGAATG CAATTGGTTT GATCATCACT 
GCCCTACCAG AGCCATATTG GATTGTTCTT CATGATCGAA TTGTGAGTGT CATCAGCAGC 
CCCAGCTTGA CGTCTGAAAC AGAGTGGGTT GGCTATCCAT TCCGCCTCTT TGATTTCACT 
GCCTGTCATC AGTCCTACTC TGAGATGAGT TGTAGCTATA CGTTAGCTCT TGCACATGCT 
GTGTGGCACC ATTCTAGCAT CGGACAACTT TCTCTCATTC CAAAGTTTCT TACTGAAGTA 
CTTCTTCCTA TAGTGAAGAC CGAATTCCAG TTGCTTTATG TATACCATCT TGTTGGACCA 
TTTTTACAAA GATTTCAGCA AGAGAGAACT CGTTGTATGA TAGAGATTGG TGTGGCGTTT 
TATGACATGC TGCTGAATGT TGACCAGTGT AGCACCCATT TAAATTACAT GGATCCCATC 
TGTGACTTCC TCTATCACAT GAAGTATATG TTTACTGGTG ACAGCGTGAA AGAGCAAGTA 
GAGAAGATTA TCTGTAACTT AAAACCAGCT TTAAAACTTC GTCTTCGATT CATCACACAC 
ATTAGCAAGA TGGAGCCAGC TGCAGTGCCT CCACAAGCCA TGAACAGTGG GTCTCCAGCA 
CCTCAGTCTA ATCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( NM_015979.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057063.2 Homo sapiens mediator complex subunit 23 (MED23), transcript variant 2, mRNA.

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CloneID	OHu19949
Clone ID Related Accession (Same CDS sequence)	NM_015979.4 , NM_015979.3
Accession Version	NM_015979.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB453121.1, AL136776.1, BC060759.1 and AW295188.1. This sequence is a reference standard in the RefSeqGene project. On Jul 14, 2012 this sequence version replaced NM_015979.2. Transcript Variant: This variant (2) contains an alternate in-frame exon and uses an alternate splice junction in the 3' end compared to variant 1. The resulting isoform (b) contains an alternate short internal segment and is shorter at the C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL136776.1, SRR1803613.71954.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGACCT TGAACATTGC TCAGCACAAG 
CAGCGCTGCC CTGTGCTGGA GGACCAGTTG GTGGATCTGG TTGTTTATGC CATGGAGCGA 
TCTGAGACCG AGGAGAAGTT TGACGATGGG GGAACAAGCC AACTCCTGTG GCAGCATCTC 
TCAAGTCAGC TCATTTTCTT TGTGCTTTTC CAGTTTGCAA GTTTTCCACA TATGGTGCTT 
TCTCTTCATC AGAAGTTAGC AGGGCGAGGA CTGATTAAAG GCAGAGATCA TCTTATGTGG 
GTTCTCCTGC AATTCATTTC TGGAAGTATT CAGAAAAATG CACTAGCTGA TTTTCTCCCT 
GTGATGAAGC TCTTCGACTT GCTATACCCA GAAAAAGAAT ATATCCCAGT TCCTGATATT 
AACAAACCCC AGTCAACCCA TGCCTTTGCA ATGACCTGTA TTTGGATTCA TCTCAATAGA 
AAAGCTCAAA ATGACAACTC CAAGCTACAG ATTCCAATAC CTCATTCCCT AAGACTTCAC 
CATGAGTTCC TGCAGCAGAG TCTAAGAAAT AAAAGTTTAC AGATGAATGA CTATAAGATT 
GCTCTATTGT GTAATGCATA CTCTACAAAT TCAGAATGTT TTACATTACC CATGGGAGCT 
CTGGTAGAAA CTATTTATGG AAATGGAATT ATGAGGATAC CTCTCCCTGG AACAAACTGT 
ATGGCTTCAG GATCTATTAC CCCCTTACCT ATGAACCTCC TGGATTCACT GACAGTTCAT 
GCCAAAATGA GCCTTATTCA CAGCATTGCA ACCAGGGTGA TAAAACTTGC TCATGCAAAG 
TCCAGTGTGG CCTTGGCTCC AGCCCTAGTG GAAACTTACA GTCGTTTATT GGTCTATATG 
GAAATAGAGT CTTTGGGCAT CAAAGGATTT ATCAGTCAGC TTTTGCCAAC TGTTTTCAAA 
TCACATGCAT GGGGGATCTT ACACACACTC CTTGAGATGT TTAGCTACCG GATGCATCAT 
ATTCAGCCTC ATTACAGAGT TCAGCTCCTG AGTCATCTTC ATACTTTGGC TGCAGTTGCA 
CAAACAAACC AGAACCAGCT CCATCTTTGT GTCGAGAGCA CTGCTCTCAG GCTTATAACA 
GCATTAGGTA GCTCAGAGGT ACAACCGCAG TTTACACGCT TCCTTAGTGA TCCCAAAACA 
GTGCTCTCAG CAGAATCTGA AGAACTGAAC CGAGCCTTGA TATTGACCTT GGCTAGAGCA 
ACTCATGTAA CAGATTTTTT TACAGGCTCT GATTCAATTC AGGGAACTTG GTGTAAAGAC 
ATACTTCAGA CCATCATGAG TTTCACTCCT CATAATTGGG CTTCACACAC CCTGAGCTGT 
TTTCCAGGCC CACTACAGGC ATTCTTCAAA CAAAATAATG TGCCTCAGGA AAGCCGTTTT 
AATCTGAAAA AAAATGTGGA GGAGGAGTAT AGGAAGTGGA AGTCAATGAG CAACGAAAAC 
GACATTATTA CCCACTTCTC TATGCAGGGC TCCCCTCCTC TCTTTCTTTG TCTTCTCTGG 
AAAATGCTCT TGGAAACAGA TCATATTAAT CAGATTGGCT ATAGAGTATT AGAGAGAATT 
GGAGCCAGGG CCTTGGTAGC CCATGTGAGG ACATTTGCAG ATTTCCTGGT ATATGAGTTT 
TCTACATCAG CAGGGGGTCA GCAACTCAAT AAATGCATTG AAATTCTTAA TGACATGGTA 
TGGAAGTATA ACATTGTTAC ACTGGACAGA TTAATTCTCT GCCTGGCCAT GCGTAGTCAC 
GAAGGAAATG AAGCCCAGGT TTGTTATTTC ATAATTCAGT TGCTGTTACT CAAACCAAAC 
GATTTTAGAA ATCGAGTAAG TGACTTTGTG AAGGAAAATT CCCCAGAGCA CTGGTTACAG 
AATGACTGGC ACACCAAGCA CATGAATTAT CACAAGAAAT ATCCAGAGAA GTTGTATTTT 
GAGGGCCTCG CGGAACAGGT GGATCCTCCT GTACAGATCC AGTCTCCCTA TCTGCCCATC 
TATTTTGGGA ATGTGTGTCT TCGATTCCTT CCAGTATTTG ATATAGTAAT CCACAGATTT 
TTAGAGTTGC TTCCGGTATC CAAATCACTG GAGACTCTAC TGGATCATCT AGGAGGCTTA 
TATAAATTTC ATGATCGTCC AGTGACTTAT CTGTATAACA CTCTGCACTA TTATGAAATG 
CACCTGAGAG ACCGCGCATT TCTCAAACGA AAACTCGTCC ATGCGATCAT TGGCTCTCTG 
AAGGATAATC GACCGCAGGG CTGGTGTCTA AGTGACACTT ACCTGAAATG CGCTATGAAT 
GCACGAGAGG AAAATCCTTG GGTTCCAGAT GACACCTACT ATTGCAGATT GATTGGCAGA 
CTAGTCGATA CGATGGCTGG CAAATCTCCT GGTCCCTTTC CAAACTGTGA CTGGAGATTC 
AATGAGTTTC CCAACCCAGC TGCCCATGCT CTCCATGTTA CTTGTGTGGA GCTCATGGCC 
TTGGCAGTTT CAGGCAAAGA AGTTGGGAAT GCCCTTCTAA ATGTTGTCCT AAAAAGTCAG 
CCTTTAGTGC CAAGAGAGAA CATTACAGCA TGGATGAATG CAATTGGTTT GATCATCACT 
GCCCTACCAG AGCCATATTG GATTGTTCTT CATGATCGAA TTGTGAGTGT CATCAGCAGC 
CCCAGCTTGA CGTCTGAAAC AGAGTGGGTT GGCTATCCAT TCCGCCTCTT TGATTTCACT 
GCCTGTCATC AGTCCTACTC TGAGATGAGT TGTAGCTATA CGTTAGCTCT TGCACATGCT 
GTGTGGCACC ATTCTAGCAT CGGACAACTT TCTCTCATTC CAAAGTTTCT TACTGAAGTA 
CTTCTTCCTA TAGTGAAGAC CGAATTCCAG TTGCTTTATG TATACCATCT TGTTGGACCA 
TTTTTACAAA GATTTCAGCA AGAGAGAACT CGTTGTATGA TAGAGATTGG TGTGGCGTTT 
TATGACATGC TGCTGAATGT TGACCAGTGT AGCACCCATT TAAATTACAT GGATCCCATC 
TGTGACTTCC TCTATCACAT GAAGTATATG TTTACTGGTG ACAGCGTGAA AGAGCAAGTA 
GAGAAGATTA TCTGTAACTT AAAACCAGCT TTAAAACTTC GTCTTCGATT CATCACACAC 
ATTAGCAAGA TGGAGCCAGC TGCAGTGCCT CCACAAGCCA TGAACAGTGG GTCTCCAGCA 
CCTCAGTCTA ATCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057063.2
CDS	191..4288
Translation	METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQTLNIAQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQ

Target ORF information:

RefSeq Version	NM_015979.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015979.3

ORF Insert Sequence:

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGACCT TGAACATTGC TCAGCACAAG 
CAGCGCTGCC CTGTGCTGGA GGACCAGTTG GTGGATCTGG TTGTTTATGC CATGGAGCGA 
TCTGAGACCG AGGAGAAGTT TGACGATGGG GGAACAAGCC AACTCCTGTG GCAGCATCTC 
TCAAGTCAGC TCATTTTCTT TGTGCTTTTC CAGTTTGCAA GTTTTCCACA TATGGTGCTT 
TCTCTTCATC AGAAGTTAGC AGGGCGAGGA CTGATTAAAG GCAGAGATCA TCTTATGTGG 
GTTCTCCTGC AATTCATTTC TGGAAGTATT CAGAAAAATG CACTAGCTGA TTTTCTCCCT 
GTGATGAAGC TCTTCGACTT GCTATACCCA GAAAAAGAAT ATATCCCAGT TCCTGATATT 
AACAAACCCC AGTCAACCCA TGCCTTTGCA ATGACCTGTA TTTGGATTCA TCTCAATAGA 
AAAGCTCAAA ATGACAACTC CAAGCTACAG ATTCCAATAC CTCATTCCCT AAGACTTCAC 
CATGAGTTCC TGCAGCAGAG TCTAAGAAAT AAAAGTTTAC AGATGAATGA CTATAAGATT 
GCTCTATTGT GTAATGCATA CTCTACAAAT TCAGAATGTT TTACATTACC CATGGGAGCT 
CTGGTAGAAA CTATTTATGG AAATGGAATT ATGAGGATAC CTCTCCCTGG AACAAACTGT 
ATGGCTTCAG GATCTATTAC CCCCTTACCT ATGAACCTCC TGGATTCACT GACAGTTCAT 
GCCAAAATGA GCCTTATTCA CAGCATTGCA ACCAGGGTGA TAAAACTTGC TCATGCAAAG 
TCCAGTGTGG CCTTGGCTCC AGCCCTAGTG GAAACTTACA GTCGTTTATT GGTCTATATG 
GAAATAGAGT CTTTGGGCAT CAAAGGATTT ATCAGTCAGC TTTTGCCAAC TGTTTTCAAA 
TCACATGCAT GGGGGATCTT ACACACACTC CTTGAGATGT TTAGCTACCG GATGCATCAT 
ATTCAGCCTC ATTACAGAGT TCAGCTCCTG AGTCATCTTC ATACTTTGGC TGCAGTTGCA 
CAAACAAACC AGAACCAGCT CCATCTTTGT GTCGAGAGCA CTGCTCTCAG GCTTATAACA 
GCATTAGGTA GCTCAGAGGT ACAACCGCAG TTTACACGCT TCCTTAGTGA TCCCAAAACA 
GTGCTCTCAG CAGAATCTGA AGAACTGAAC CGAGCCTTGA TATTGACCTT GGCTAGAGCA 
ACTCATGTAA CAGATTTTTT TACAGGCTCT GATTCAATTC AGGGAACTTG GTGTAAAGAC 
ATACTTCAGA CCATCATGAG TTTCACTCCT CATAATTGGG CTTCACACAC CCTGAGCTGT 
TTTCCAGGCC CACTACAGGC ATTCTTCAAA CAAAATAATG TGCCTCAGGA AAGCCGTTTT 
AATCTGAAAA AAAATGTGGA GGAGGAGTAT AGGAAGTGGA AGTCAATGAG CAACGAAAAC 
GACATTATTA CCCACTTCTC TATGCAGGGC TCCCCTCCTC TCTTTCTTTG TCTTCTCTGG 
AAAATGCTCT TGGAAACAGA TCATATTAAT CAGATTGGCT ATAGAGTATT AGAGAGAATT 
GGAGCCAGGG CCTTGGTAGC CCATGTGAGG ACATTTGCAG ATTTCCTGGT ATATGAGTTT 
TCTACATCAG CAGGGGGTCA GCAACTCAAT AAATGCATTG AAATTCTTAA TGACATGGTA 
TGGAAGTATA ACATTGTTAC ACTGGACAGA TTAATTCTCT GCCTGGCCAT GCGTAGTCAC 
GAAGGAAATG AAGCCCAGGT TTGTTATTTC ATAATTCAGT TGCTGTTACT CAAACCAAAC 
GATTTTAGAA ATCGAGTAAG TGACTTTGTG AAGGAAAATT CCCCAGAGCA CTGGTTACAG 
AATGACTGGC ACACCAAGCA CATGAATTAT CACAAGAAAT ATCCAGAGAA GTTGTATTTT 
GAGGGCCTCG CGGAACAGGT GGATCCTCCT GTACAGATCC AGTCTCCCTA TCTGCCCATC 
TATTTTGGGA ATGTGTGTCT TCGATTCCTT CCAGTATTTG ATATAGTAAT CCACAGATTT 
TTAGAGTTGC TTCCGGTATC CAAATCACTG GAGACTCTAC TGGATCATCT AGGAGGCTTA 
TATAAATTTC ATGATCGTCC AGTGACTTAT CTGTATAACA CTCTGCACTA TTATGAAATG 
CACCTGAGAG ACCGCGCATT TCTCAAACGA AAACTCGTCC ATGCGATCAT TGGCTCTCTG 
AAGGATAATC GACCGCAGGG CTGGTGTCTA AGTGACACTT ACCTGAAATG CGCTATGAAT 
GCACGAGAGG AAAATCCTTG GGTTCCAGAT GACACCTACT ATTGCAGATT GATTGGCAGA 
CTAGTCGATA CGATGGCTGG CAAATCTCCT GGTCCCTTTC CAAACTGTGA CTGGAGATTC 
AATGAGTTTC CCAACCCAGC TGCCCATGCT CTCCATGTTA CTTGTGTGGA GCTCATGGCC 
TTGGCAGTTT CAGGCAAAGA AGTTGGGAAT GCCCTTCTAA ATGTTGTCCT AAAAAGTCAG 
CCTTTAGTGC CAAGAGAGAA CATTACAGCA TGGATGAATG CAATTGGTTT GATCATCACT 
GCCCTACCAG AGCCATATTG GATTGTTCTT CATGATCGAA TTGTGAGTGT CATCAGCAGC 
CCCAGCTTGA CGTCTGAAAC AGAGTGGGTT GGCTATCCAT TCCGCCTCTT TGATTTCACT 
GCCTGTCATC AGTCCTACTC TGAGATGAGT TGTAGCTATA CGTTAGCTCT TGCACATGCT 
GTGTGGCACC ATTCTAGCAT CGGACAACTT TCTCTCATTC CAAAGTTTCT TACTGAAGTA 
CTTCTTCCTA TAGTGAAGAC CGAATTCCAG TTGCTTTATG TATACCATCT TGTTGGACCA 
TTTTTACAAA GATTTCAGCA AGAGAGAACT CGTTGTATGA TAGAGATTGG TGTGGCGTTT 
TATGACATGC TGCTGAATGT TGACCAGTGT AGCACCCATT TAAATTACAT GGATCCCATC 
TGTGACTTCC TCTATCACAT GAAGTATATG TTTACTGGTG ACAGCGTGAA AGAGCAAGTA 
GAGAAGATTA TCTGTAACTT AAAACCAGCT TTAAAACTTC GTCTTCGATT CATCACACAC 
ATTAGCAAGA TGGAGCCAGC TGCAGTGCCT CCACAAGCCA TGAACAGTGG GTCTCCAGCA 
CCTCAGTCTA ATCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( XM_005267223.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005267280.1 Homo sapiens mediator complex subunit 23 (MED23), transcript variant X1, mRNA.

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CloneID	OHu41025
Clone ID Related Accession (Same CDS sequence)	XM_005267223.3 , NM_001376517.1 , XM_005267223.2
Accession Version	XM_005267223.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005267223.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGACCT TGAACATTGC TCAGCACAAG 
CAGCGCTGCC CTGTGCTGGA GGACCAGTTG GTGGATCTGG TTGTTTATGC CATGGAGCGA 
TCTGAGACCG AGGAGAAGTT TGACGATGGG GGAACAAGCC AACTCCTGTG GCAGCATCTC 
TCAAGTCAGC TCATTTTCTT TGTGCTTTTC CAGTTTGCAA GTTTTCCACA TATGGTGCTT 
TCTCTTCATC AGAAGTTAGC AGGGCGAGGA CTGATTAAAG GCAGAGATCA TCTTATGTGG 
GTTCTCCTGC AATTCATTTC TGGAAGTATT CAGAAAAATG CACTAGCTGA TTTTCTCCCT 
GTGATGAAGC TCTTCGACTT GCTATACCCA GAAAAAGAAT ATATCCCAGT TCCTGATATT 
AACAAACCCC AGTCAACCCA TGCCTTTGCA ATGACCTGTA TTTGGATTCA TCTCAATAGA 
AAAGCTCAAA ATGACAACTC CAAGCTACAG ATTCCAATAC CTCATTCCCT AAGACTTCAC 
CATGAGTTCC TGCAGCAGAG TCTAAGAAAT AAAAGTTTAC AGATGAATGA CTATAAGATT 
GCTCTATTGT GTAATGCATA CTCTACAAAT TCAGAATGTT TTACATTACC CATGGGAGCT 
CTGGTAGAAA CTATTTATGG AAATGGAATT ATGAGGATAC CTCTCCCTGG AACAAACTGT 
ATGGCTTCAG GATCTATTAC CCCCTTACCT ATGAACCTCC TGGATTCACT GACAGTTCAT 
GCCAAAATGA GCCTTATTCA CAGCATTGCA ACCAGGGTGA TAAAACTTGC TCATGCAAAG 
TCCAGTGTGG CCTTGGCTCC AGCCCTAGTG GAAACTTACA GTCGTTTATT GGTCTATATG 
GAAATAGAGT CTTTGGGCAT CAAAGGATTT ATCAGTCAGC TTTTGCCAAC TGTTTTCAAA 
TCACATGCAT GGGGGATCTT ACACACACTC CTTGAGATGT TTAGCTACCG GATGCATCAT 
ATTCAGCCTC ATTACAGAGT TCAGCTCCTG AGTCATCTTC ATACTTTGGC TGCAGTTGCA 
CAAACAAACC AGAACCAGCT CCATCTTTGT GTCGAGAGCA CTGCTCTCAG GCTTATAACA 
GCATTAGGTA GCTCAGAGGT ACAACCGCAG TTTACACGCT TCCTTAGTGA TCCCAAAACA 
GTGCTCTCAG CAGAATCTGA AGAACTGAAC CGAGCCTTGA TATTGACCTT GGCTAGAGCA 
ACTCATGTAA CAGATTTTTT TACAGGCTCT GATTCAATTC AGGGAACTTG GTGTAAAGAC 
ATACTTCAGA CCATCATGAG TTTCACTCCT CATAATTGGG CTTCACACAC CCTGAGCTGT 
TTTCCAGGCC CACTACAGGC ATTCTTCAAA CAAAATAATG TGCCTCAGGA AAGCCGTTTT 
AATCTGAAAA AAAATGTGGA GGAGGAGTAT AGGAAGTGGA AGTCAATGAG CAACGAAAAC 
GACATTATTA CCCACTTCTC TATGCAGGGC TCCCCTCCTC TCTTTCTTTG TCTTCTCTGG 
AAAATGCTCT TGGAAACAGA TCATATTAAT CAGATTGGCT ATAGAGTATT AGAGAGAATT 
GGAGCCAGGG CCTTGGTAGC CCATGTGAGG ACATTTGCAG ATTTCCTGGT ATATGAGTTT 
TCTACATCAG CAGGGGGTCA GCAACTCAAT AAATGCATTG AAATTCTTAA TGACATGGTA 
TGGAAGTATA ACATTGTTAC ACTGGACAGA TTAATTCTCT GCCTGGCCAT GCGTAGTCAC 
GAAGGAAATG AAGCCCAGGT TTGTTATTTC ATAATTCAGT TGCTGTTACT CAAACCAAAC 
GATTTTAGAA ATCGAGTAAG TGACTTTGTG AAGGAAAATT CCCCAGAGCA CTGGTTACAG 
AATGACTGGC ACACCAAGCA CATGAATTAT CACAAGAAAT ATCCAGAGAA GTTGTATTTT 
GAGGGCCTCG CGGAACAGGT GGATCCTCCT GTACAGATCC AGTCTCCCTA TCTGCCCATC 
TATTTTGGGA ATGTGTGTCT TCGATTCCTT CCAGTATTTG ATATAGTAAT CCACAGATTT 
TTAGAGTTGC TTCCGGTATC CAAATCACTG GAGACTCTAC TGGATCATCT AGGAGGCTTA 
TATAAATTTC ATGATCGTCC AGTGACTTAT CTGTATAACA CTCTGCACTA TTATGAAATG 
CACCTGAGAG ACCGCGCATT TCTCAAACGA AAACTCGTCC ATGCGATCAT TGGCTCTCTG 
AAGGATAATC GACCGCAGGG CTGGTGTCTA AGTGACACTT ACCTGAAATG CGCTATGAAT 
GCACGAGAGG AAAATCCTTG GGTTCCAGAT GACACCTACT ATTGCAGATT GATTGGCAGA 
CTAGTCGATA CGATGGCTGG CAAATCTCCT GGTCCCTTTC CAAACTGTGA CTGGAGATTC 
AATGAGTTTC CCAACCCAGC TGCCCATGCT CTCCATGTTA CTTGTGTGGA GCTCATGGCC 
TTGGCAGTTT CAGGCAAAGA AGTTGGGAAT GCCCTTCTAA ATGTTGTCCT AAAAAGTCAG 
CCTTTAGTGC CAAGAGAGAA CATTACAGCA TGGATGAATG CAATTGGTTT GATCATCACT 
GCCCTACCAG AGCCATATTG GATTGTTCTT CATGATCGAA TTGTGAGTGT CATCAGCAGC 
CCCAGCTTGA CGTCTGAAAC AGAGTGGGTT GGCTATCCAT TCCGCCTCTT TGATTTCACT 
GCCTGTCATC AGTCCTACTC TGAGATGAGT TGTAGCTATA CGTTAGCTCT TGCACATGCT 
GTGTGGCACC ATTCTAGCAT CGGACAACTT TCTCTCATTC CAAAGTTTCT TACTGAAGTA 
CTTCTTCCTA TAGTGAAGAC CGAATTCCAG TTGCTTTATG TATACCATCT TGTTGGACCA 
TTTTTACAAA GATTTCAGCA AGAGAGAACT CGTTGTATGA TAGAGATTGG TGTGGCGTTT 
TATGACATGC TGCTGAATGT TGACCAGTGT AGCACCCATT TAAATTACAT GGATCCCATC 
TGTGACTTCC TCTATCACAT GAAGTATATG TTTACTGGTG ACAGCGTGAA AGAGCAAGTA 
GAGAAGATTA TCTGTAACTT AAAACCAGCT TTAAAACTTC GTCTTCGATT CATCACACAC 
ATTAGCAAGA TGGAGCCAGC TGCAGTGCCT CCACAAGCCA TGAACAGTGG GTCTCCAGCA 
CCTCAGTCTA ATCAGGTGCC CGTGTCTTTA CCAGTAACTC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005267280.1
CDS	183..4307
Translation	METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQTLNIAQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQVPVSLPVTQ

Target ORF information:

RefSeq Version	XM_005267223.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005267223.3

ORF Insert Sequence:

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGACCT TGAACATTGC TCAGCACAAG 
CAGCGCTGCC CTGTGCTGGA GGACCAGTTG GTGGATCTGG TTGTTTATGC CATGGAGCGA 
TCTGAGACCG AGGAGAAGTT TGACGATGGG GGAACAAGCC AACTCCTGTG GCAGCATCTC 
TCAAGTCAGC TCATTTTCTT TGTGCTTTTC CAGTTTGCAA GTTTTCCACA TATGGTGCTT 
TCTCTTCATC AGAAGTTAGC AGGGCGAGGA CTGATTAAAG GCAGAGATCA TCTTATGTGG 
GTTCTCCTGC AATTCATTTC TGGAAGTATT CAGAAAAATG CACTAGCTGA TTTTCTCCCT 
GTGATGAAGC TCTTCGACTT GCTATACCCA GAAAAAGAAT ATATCCCAGT TCCTGATATT 
AACAAACCCC AGTCAACCCA TGCCTTTGCA ATGACCTGTA TTTGGATTCA TCTCAATAGA 
AAAGCTCAAA ATGACAACTC CAAGCTACAG ATTCCAATAC CTCATTCCCT AAGACTTCAC 
CATGAGTTCC TGCAGCAGAG TCTAAGAAAT AAAAGTTTAC AGATGAATGA CTATAAGATT 
GCTCTATTGT GTAATGCATA CTCTACAAAT TCAGAATGTT TTACATTACC CATGGGAGCT 
CTGGTAGAAA CTATTTATGG AAATGGAATT ATGAGGATAC CTCTCCCTGG AACAAACTGT 
ATGGCTTCAG GATCTATTAC CCCCTTACCT ATGAACCTCC TGGATTCACT GACAGTTCAT 
GCCAAAATGA GCCTTATTCA CAGCATTGCA ACCAGGGTGA TAAAACTTGC TCATGCAAAG 
TCCAGTGTGG CCTTGGCTCC AGCCCTAGTG GAAACTTACA GTCGTTTATT GGTCTATATG 
GAAATAGAGT CTTTGGGCAT CAAAGGATTT ATCAGTCAGC TTTTGCCAAC TGTTTTCAAA 
TCACATGCAT GGGGGATCTT ACACACACTC CTTGAGATGT TTAGCTACCG GATGCATCAT 
ATTCAGCCTC ATTACAGAGT TCAGCTCCTG AGTCATCTTC ATACTTTGGC TGCAGTTGCA 
CAAACAAACC AGAACCAGCT CCATCTTTGT GTCGAGAGCA CTGCTCTCAG GCTTATAACA 
GCATTAGGTA GCTCAGAGGT ACAACCGCAG TTTACACGCT TCCTTAGTGA TCCCAAAACA 
GTGCTCTCAG CAGAATCTGA AGAACTGAAC CGAGCCTTGA TATTGACCTT GGCTAGAGCA 
ACTCATGTAA CAGATTTTTT TACAGGCTCT GATTCAATTC AGGGAACTTG GTGTAAAGAC 
ATACTTCAGA CCATCATGAG TTTCACTCCT CATAATTGGG CTTCACACAC CCTGAGCTGT 
TTTCCAGGCC CACTACAGGC ATTCTTCAAA CAAAATAATG TGCCTCAGGA AAGCCGTTTT 
AATCTGAAAA AAAATGTGGA GGAGGAGTAT AGGAAGTGGA AGTCAATGAG CAACGAAAAC 
GACATTATTA CCCACTTCTC TATGCAGGGC TCCCCTCCTC TCTTTCTTTG TCTTCTCTGG 
AAAATGCTCT TGGAAACAGA TCATATTAAT CAGATTGGCT ATAGAGTATT AGAGAGAATT 
GGAGCCAGGG CCTTGGTAGC CCATGTGAGG ACATTTGCAG ATTTCCTGGT ATATGAGTTT 
TCTACATCAG CAGGGGGTCA GCAACTCAAT AAATGCATTG AAATTCTTAA TGACATGGTA 
TGGAAGTATA ACATTGTTAC ACTGGACAGA TTAATTCTCT GCCTGGCCAT GCGTAGTCAC 
GAAGGAAATG AAGCCCAGGT TTGTTATTTC ATAATTCAGT TGCTGTTACT CAAACCAAAC 
GATTTTAGAA ATCGAGTAAG TGACTTTGTG AAGGAAAATT CCCCAGAGCA CTGGTTACAG 
AATGACTGGC ACACCAAGCA CATGAATTAT CACAAGAAAT ATCCAGAGAA GTTGTATTTT 
GAGGGCCTCG CGGAACAGGT GGATCCTCCT GTACAGATCC AGTCTCCCTA TCTGCCCATC 
TATTTTGGGA ATGTGTGTCT TCGATTCCTT CCAGTATTTG ATATAGTAAT CCACAGATTT 
TTAGAGTTGC TTCCGGTATC CAAATCACTG GAGACTCTAC TGGATCATCT AGGAGGCTTA 
TATAAATTTC ATGATCGTCC AGTGACTTAT CTGTATAACA CTCTGCACTA TTATGAAATG 
CACCTGAGAG ACCGCGCATT TCTCAAACGA AAACTCGTCC ATGCGATCAT TGGCTCTCTG 
AAGGATAATC GACCGCAGGG CTGGTGTCTA AGTGACACTT ACCTGAAATG CGCTATGAAT 
GCACGAGAGG AAAATCCTTG GGTTCCAGAT GACACCTACT ATTGCAGATT GATTGGCAGA 
CTAGTCGATA CGATGGCTGG CAAATCTCCT GGTCCCTTTC CAAACTGTGA CTGGAGATTC 
AATGAGTTTC CCAACCCAGC TGCCCATGCT CTCCATGTTA CTTGTGTGGA GCTCATGGCC 
TTGGCAGTTT CAGGCAAAGA AGTTGGGAAT GCCCTTCTAA ATGTTGTCCT AAAAAGTCAG 
CCTTTAGTGC CAAGAGAGAA CATTACAGCA TGGATGAATG CAATTGGTTT GATCATCACT 
GCCCTACCAG AGCCATATTG GATTGTTCTT CATGATCGAA TTGTGAGTGT CATCAGCAGC 
CCCAGCTTGA CGTCTGAAAC AGAGTGGGTT GGCTATCCAT TCCGCCTCTT TGATTTCACT 
GCCTGTCATC AGTCCTACTC TGAGATGAGT TGTAGCTATA CGTTAGCTCT TGCACATGCT 
GTGTGGCACC ATTCTAGCAT CGGACAACTT TCTCTCATTC CAAAGTTTCT TACTGAAGTA 
CTTCTTCCTA TAGTGAAGAC CGAATTCCAG TTGCTTTATG TATACCATCT TGTTGGACCA 
TTTTTACAAA GATTTCAGCA AGAGAGAACT CGTTGTATGA TAGAGATTGG TGTGGCGTTT 
TATGACATGC TGCTGAATGT TGACCAGTGT AGCACCCATT TAAATTACAT GGATCCCATC 
TGTGACTTCC TCTATCACAT GAAGTATATG TTTACTGGTG ACAGCGTGAA AGAGCAAGTA 
GAGAAGATTA TCTGTAACTT AAAACCAGCT TTAAAACTTC GTCTTCGATT CATCACACAC 
ATTAGCAAGA TGGAGCCAGC TGCAGTGCCT CCACAAGCCA TGAACAGTGG GTCTCCAGCA 
CCTCAGTCTA ATCAGGTGCC CGTGTCTTTA CCAGTAACTC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( XM_005267223.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005267280.1 Homo sapiens mediator complex subunit 23 (MED23), transcript variant X1, mRNA.

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CloneID	OHu41025
Clone ID Related Accession (Same CDS sequence)	XM_005267223.3 , NM_001376517.1 , XM_005267223.2
Accession Version	XM_005267223.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005267223.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGACCT TGAACATTGC TCAGCACAAG 
CAGCGCTGCC CTGTGCTGGA GGACCAGTTG GTGGATCTGG TTGTTTATGC CATGGAGCGA 
TCTGAGACCG AGGAGAAGTT TGACGATGGG GGAACAAGCC AACTCCTGTG GCAGCATCTC 
TCAAGTCAGC TCATTTTCTT TGTGCTTTTC CAGTTTGCAA GTTTTCCACA TATGGTGCTT 
TCTCTTCATC AGAAGTTAGC AGGGCGAGGA CTGATTAAAG GCAGAGATCA TCTTATGTGG 
GTTCTCCTGC AATTCATTTC TGGAAGTATT CAGAAAAATG CACTAGCTGA TTTTCTCCCT 
GTGATGAAGC TCTTCGACTT GCTATACCCA GAAAAAGAAT ATATCCCAGT TCCTGATATT 
AACAAACCCC AGTCAACCCA TGCCTTTGCA ATGACCTGTA TTTGGATTCA TCTCAATAGA 
AAAGCTCAAA ATGACAACTC CAAGCTACAG ATTCCAATAC CTCATTCCCT AAGACTTCAC 
CATGAGTTCC TGCAGCAGAG TCTAAGAAAT AAAAGTTTAC AGATGAATGA CTATAAGATT 
GCTCTATTGT GTAATGCATA CTCTACAAAT TCAGAATGTT TTACATTACC CATGGGAGCT 
CTGGTAGAAA CTATTTATGG AAATGGAATT ATGAGGATAC CTCTCCCTGG AACAAACTGT 
ATGGCTTCAG GATCTATTAC CCCCTTACCT ATGAACCTCC TGGATTCACT GACAGTTCAT 
GCCAAAATGA GCCTTATTCA CAGCATTGCA ACCAGGGTGA TAAAACTTGC TCATGCAAAG 
TCCAGTGTGG CCTTGGCTCC AGCCCTAGTG GAAACTTACA GTCGTTTATT GGTCTATATG 
GAAATAGAGT CTTTGGGCAT CAAAGGATTT ATCAGTCAGC TTTTGCCAAC TGTTTTCAAA 
TCACATGCAT GGGGGATCTT ACACACACTC CTTGAGATGT TTAGCTACCG GATGCATCAT 
ATTCAGCCTC ATTACAGAGT TCAGCTCCTG AGTCATCTTC ATACTTTGGC TGCAGTTGCA 
CAAACAAACC AGAACCAGCT CCATCTTTGT GTCGAGAGCA CTGCTCTCAG GCTTATAACA 
GCATTAGGTA GCTCAGAGGT ACAACCGCAG TTTACACGCT TCCTTAGTGA TCCCAAAACA 
GTGCTCTCAG CAGAATCTGA AGAACTGAAC CGAGCCTTGA TATTGACCTT GGCTAGAGCA 
ACTCATGTAA CAGATTTTTT TACAGGCTCT GATTCAATTC AGGGAACTTG GTGTAAAGAC 
ATACTTCAGA CCATCATGAG TTTCACTCCT CATAATTGGG CTTCACACAC CCTGAGCTGT 
TTTCCAGGCC CACTACAGGC ATTCTTCAAA CAAAATAATG TGCCTCAGGA AAGCCGTTTT 
AATCTGAAAA AAAATGTGGA GGAGGAGTAT AGGAAGTGGA AGTCAATGAG CAACGAAAAC 
GACATTATTA CCCACTTCTC TATGCAGGGC TCCCCTCCTC TCTTTCTTTG TCTTCTCTGG 
AAAATGCTCT TGGAAACAGA TCATATTAAT CAGATTGGCT ATAGAGTATT AGAGAGAATT 
GGAGCCAGGG CCTTGGTAGC CCATGTGAGG ACATTTGCAG ATTTCCTGGT ATATGAGTTT 
TCTACATCAG CAGGGGGTCA GCAACTCAAT AAATGCATTG AAATTCTTAA TGACATGGTA 
TGGAAGTATA ACATTGTTAC ACTGGACAGA TTAATTCTCT GCCTGGCCAT GCGTAGTCAC 
GAAGGAAATG AAGCCCAGGT TTGTTATTTC ATAATTCAGT TGCTGTTACT CAAACCAAAC 
GATTTTAGAA ATCGAGTAAG TGACTTTGTG AAGGAAAATT CCCCAGAGCA CTGGTTACAG 
AATGACTGGC ACACCAAGCA CATGAATTAT CACAAGAAAT ATCCAGAGAA GTTGTATTTT 
GAGGGCCTCG CGGAACAGGT GGATCCTCCT GTACAGATCC AGTCTCCCTA TCTGCCCATC 
TATTTTGGGA ATGTGTGTCT TCGATTCCTT CCAGTATTTG ATATAGTAAT CCACAGATTT 
TTAGAGTTGC TTCCGGTATC CAAATCACTG GAGACTCTAC TGGATCATCT AGGAGGCTTA 
TATAAATTTC ATGATCGTCC AGTGACTTAT CTGTATAACA CTCTGCACTA TTATGAAATG 
CACCTGAGAG ACCGCGCATT TCTCAAACGA AAACTCGTCC ATGCGATCAT TGGCTCTCTG 
AAGGATAATC GACCGCAGGG CTGGTGTCTA AGTGACACTT ACCTGAAATG CGCTATGAAT 
GCACGAGAGG AAAATCCTTG GGTTCCAGAT GACACCTACT ATTGCAGATT GATTGGCAGA 
CTAGTCGATA CGATGGCTGG CAAATCTCCT GGTCCCTTTC CAAACTGTGA CTGGAGATTC 
AATGAGTTTC CCAACCCAGC TGCCCATGCT CTCCATGTTA CTTGTGTGGA GCTCATGGCC 
TTGGCAGTTT CAGGCAAAGA AGTTGGGAAT GCCCTTCTAA ATGTTGTCCT AAAAAGTCAG 
CCTTTAGTGC CAAGAGAGAA CATTACAGCA TGGATGAATG CAATTGGTTT GATCATCACT 
GCCCTACCAG AGCCATATTG GATTGTTCTT CATGATCGAA TTGTGAGTGT CATCAGCAGC 
CCCAGCTTGA CGTCTGAAAC AGAGTGGGTT GGCTATCCAT TCCGCCTCTT TGATTTCACT 
GCCTGTCATC AGTCCTACTC TGAGATGAGT TGTAGCTATA CGTTAGCTCT TGCACATGCT 
GTGTGGCACC ATTCTAGCAT CGGACAACTT TCTCTCATTC CAAAGTTTCT TACTGAAGTA 
CTTCTTCCTA TAGTGAAGAC CGAATTCCAG TTGCTTTATG TATACCATCT TGTTGGACCA 
TTTTTACAAA GATTTCAGCA AGAGAGAACT CGTTGTATGA TAGAGATTGG TGTGGCGTTT 
TATGACATGC TGCTGAATGT TGACCAGTGT AGCACCCATT TAAATTACAT GGATCCCATC 
TGTGACTTCC TCTATCACAT GAAGTATATG TTTACTGGTG ACAGCGTGAA AGAGCAAGTA 
GAGAAGATTA TCTGTAACTT AAAACCAGCT TTAAAACTTC GTCTTCGATT CATCACACAC 
ATTAGCAAGA TGGAGCCAGC TGCAGTGCCT CCACAAGCCA TGAACAGTGG GTCTCCAGCA 
CCTCAGTCTA ATCAGGTGCC CGTGTCTTTA CCAGTAACTC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005267280.1
CDS	187..4311
Translation	METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQTLNIAQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQVPVSLPVTQ

Target ORF information:

RefSeq Version	XM_005267223.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005267223.2

ORF Insert Sequence:

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
GATCTGTTTG AACCACAGAC TGCTTTGTTG AGATATGTAT TGGAGCAGCC TTATTCCAGG 
GATATGGTCT GCAATATGCT AGGTTTAAAT AAGCAGACCT TGAACATTGC TCAGCACAAG 
CAGCGCTGCC CTGTGCTGGA GGACCAGTTG GTGGATCTGG TTGTTTATGC CATGGAGCGA 
TCTGAGACCG AGGAGAAGTT TGACGATGGG GGAACAAGCC AACTCCTGTG GCAGCATCTC 
TCAAGTCAGC TCATTTTCTT TGTGCTTTTC CAGTTTGCAA GTTTTCCACA TATGGTGCTT 
TCTCTTCATC AGAAGTTAGC AGGGCGAGGA CTGATTAAAG GCAGAGATCA TCTTATGTGG 
GTTCTCCTGC AATTCATTTC TGGAAGTATT CAGAAAAATG CACTAGCTGA TTTTCTCCCT 
GTGATGAAGC TCTTCGACTT GCTATACCCA GAAAAAGAAT ATATCCCAGT TCCTGATATT 
AACAAACCCC AGTCAACCCA TGCCTTTGCA ATGACCTGTA TTTGGATTCA TCTCAATAGA 
AAAGCTCAAA ATGACAACTC CAAGCTACAG ATTCCAATAC CTCATTCCCT AAGACTTCAC 
CATGAGTTCC TGCAGCAGAG TCTAAGAAAT AAAAGTTTAC AGATGAATGA CTATAAGATT 
GCTCTATTGT GTAATGCATA CTCTACAAAT TCAGAATGTT TTACATTACC CATGGGAGCT 
CTGGTAGAAA CTATTTATGG AAATGGAATT ATGAGGATAC CTCTCCCTGG AACAAACTGT 
ATGGCTTCAG GATCTATTAC CCCCTTACCT ATGAACCTCC TGGATTCACT GACAGTTCAT 
GCCAAAATGA GCCTTATTCA CAGCATTGCA ACCAGGGTGA TAAAACTTGC TCATGCAAAG 
TCCAGTGTGG CCTTGGCTCC AGCCCTAGTG GAAACTTACA GTCGTTTATT GGTCTATATG 
GAAATAGAGT CTTTGGGCAT CAAAGGATTT ATCAGTCAGC TTTTGCCAAC TGTTTTCAAA 
TCACATGCAT GGGGGATCTT ACACACACTC CTTGAGATGT TTAGCTACCG GATGCATCAT 
ATTCAGCCTC ATTACAGAGT TCAGCTCCTG AGTCATCTTC ATACTTTGGC TGCAGTTGCA 
CAAACAAACC AGAACCAGCT CCATCTTTGT GTCGAGAGCA CTGCTCTCAG GCTTATAACA 
GCATTAGGTA GCTCAGAGGT ACAACCGCAG TTTACACGCT TCCTTAGTGA TCCCAAAACA 
GTGCTCTCAG CAGAATCTGA AGAACTGAAC CGAGCCTTGA TATTGACCTT GGCTAGAGCA 
ACTCATGTAA CAGATTTTTT TACAGGCTCT GATTCAATTC AGGGAACTTG GTGTAAAGAC 
ATACTTCAGA CCATCATGAG TTTCACTCCT CATAATTGGG CTTCACACAC CCTGAGCTGT 
TTTCCAGGCC CACTACAGGC ATTCTTCAAA CAAAATAATG TGCCTCAGGA AAGCCGTTTT 
AATCTGAAAA AAAATGTGGA GGAGGAGTAT AGGAAGTGGA AGTCAATGAG CAACGAAAAC 
GACATTATTA CCCACTTCTC TATGCAGGGC TCCCCTCCTC TCTTTCTTTG TCTTCTCTGG 
AAAATGCTCT TGGAAACAGA TCATATTAAT CAGATTGGCT ATAGAGTATT AGAGAGAATT 
GGAGCCAGGG CCTTGGTAGC CCATGTGAGG ACATTTGCAG ATTTCCTGGT ATATGAGTTT 
TCTACATCAG CAGGGGGTCA GCAACTCAAT AAATGCATTG AAATTCTTAA TGACATGGTA 
TGGAAGTATA ACATTGTTAC ACTGGACAGA TTAATTCTCT GCCTGGCCAT GCGTAGTCAC 
GAAGGAAATG AAGCCCAGGT TTGTTATTTC ATAATTCAGT TGCTGTTACT CAAACCAAAC 
GATTTTAGAA ATCGAGTAAG TGACTTTGTG AAGGAAAATT CCCCAGAGCA CTGGTTACAG 
AATGACTGGC ACACCAAGCA CATGAATTAT CACAAGAAAT ATCCAGAGAA GTTGTATTTT 
GAGGGCCTCG CGGAACAGGT GGATCCTCCT GTACAGATCC AGTCTCCCTA TCTGCCCATC 
TATTTTGGGA ATGTGTGTCT TCGATTCCTT CCAGTATTTG ATATAGTAAT CCACAGATTT 
TTAGAGTTGC TTCCGGTATC CAAATCACTG GAGACTCTAC TGGATCATCT AGGAGGCTTA 
TATAAATTTC ATGATCGTCC AGTGACTTAT CTGTATAACA CTCTGCACTA TTATGAAATG 
CACCTGAGAG ACCGCGCATT TCTCAAACGA AAACTCGTCC ATGCGATCAT TGGCTCTCTG 
AAGGATAATC GACCGCAGGG CTGGTGTCTA AGTGACACTT ACCTGAAATG CGCTATGAAT 
GCACGAGAGG AAAATCCTTG GGTTCCAGAT GACACCTACT ATTGCAGATT GATTGGCAGA 
CTAGTCGATA CGATGGCTGG CAAATCTCCT GGTCCCTTTC CAAACTGTGA CTGGAGATTC 
AATGAGTTTC CCAACCCAGC TGCCCATGCT CTCCATGTTA CTTGTGTGGA GCTCATGGCC 
TTGGCAGTTT CAGGCAAAGA AGTTGGGAAT GCCCTTCTAA ATGTTGTCCT AAAAAGTCAG 
CCTTTAGTGC CAAGAGAGAA CATTACAGCA TGGATGAATG CAATTGGTTT GATCATCACT 
GCCCTACCAG AGCCATATTG GATTGTTCTT CATGATCGAA TTGTGAGTGT CATCAGCAGC 
CCCAGCTTGA CGTCTGAAAC AGAGTGGGTT GGCTATCCAT TCCGCCTCTT TGATTTCACT 
GCCTGTCATC AGTCCTACTC TGAGATGAGT TGTAGCTATA CGTTAGCTCT TGCACATGCT 
GTGTGGCACC ATTCTAGCAT CGGACAACTT TCTCTCATTC CAAAGTTTCT TACTGAAGTA 
CTTCTTCCTA TAGTGAAGAC CGAATTCCAG TTGCTTTATG TATACCATCT TGTTGGACCA 
TTTTTACAAA GATTTCAGCA AGAGAGAACT CGTTGTATGA TAGAGATTGG TGTGGCGTTT 
TATGACATGC TGCTGAATGT TGACCAGTGT AGCACCCATT TAAATTACAT GGATCCCATC 
TGTGACTTCC TCTATCACAT GAAGTATATG TTTACTGGTG ACAGCGTGAA AGAGCAAGTA 
GAGAAGATTA TCTGTAACTT AAAACCAGCT TTAAAACTTC GTCTTCGATT CATCACACAC 
ATTAGCAAGA TGGAGCCAGC TGCAGTGCCT CCACAAGCCA TGAACAGTGG GTCTCCAGCA 
CCTCAGTCTA ATCAGGTGCC CGTGTCTTTA CCAGTAACTC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( XM_011536257.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534559.1 Homo sapiens mediator complex subunit 23 (MED23), transcript variant X2, mRNA.

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CloneID	OHu62822
Clone ID Related Accession (Same CDS sequence)	XM_011536257.2 , XM_011536257.3
Accession Version	XM_011536257.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011536257.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
CACAAGCAGC GCTGCCCTGT GCTGGAGGAC CAGTTGGTGG ATCTGGTTGT TTATGCCATG 
GAGCGATCTG AGACCGAGGA GAAGTTTGAC GATGGGGGAA CAAGCCAACT CCTGTGGCAG 
CATCTCTCAA GTCAGCTCAT TTTCTTTGTG CTTTTCCAGT TTGCAAGTTT TCCACATATG 
GTGCTTTCTC TTCATCAGAA GTTAGCAGGG CGAGGACTGA TTAAAGGCAG AGATCATCTT 
ATGTGGGTTC TCCTGCAATT CATTTCTGGA AGTATTCAGA AAAATGCACT AGCTGATTTT 
CTCCCTGTGA TGAAGCTCTT CGACTTGCTA TACCCAGAAA AAGAATATAT CCCAGTTCCT 
GATATTAACA AACCCCAGTC AACCCATGCC TTTGCAATGA CCTGTATTTG GATTCATCTC 
AATAGAAAAG CTCAAAATGA CAACTCCAAG CTACAGATTC CAATACCTCA TTCCCTAAGA 
CTTCACCATG AGTTCCTGCA GCAGAGTCTA AGAAATAAAA GTTTACAGAT GAATGACTAT 
AAGATTGCTC TATTGTGTAA TGCATACTCT ACAAATTCAG AATGTTTTAC ATTACCCATG 
GGAGCTCTGG TAGAAACTAT TTATGGAAAT GGAATTATGA GGATACCTCT CCCTGGAACA 
AACTGTATGG CTTCAGGATC TATTACCCCC TTACCTATGA ACCTCCTGGA TTCACTGACA 
GTTCATGCCA AAATGAGCCT TATTCACAGC ATTGCAACCA GGGTGATAAA ACTTGCTCAT 
GCAAAGTCCA GTGTGGCCTT GGCTCCAGCC CTAGTGGAAA CTTACAGTCG TTTATTGGTC 
TATATGGAAA TAGAGTCTTT GGGCATCAAA GGATTTATCA GTCAGCTTTT GCCAACTGTT 
TTCAAATCAC ATGCATGGGG GATCTTACAC ACACTCCTTG AGATGTTTAG CTACCGGATG 
CATCATATTC AGCCTCATTA CAGAGTTCAG CTCCTGAGTC ATCTTCATAC TTTGGCTGCA 
GTTGCACAAA CAAACCAGAA CCAGCTCCAT CTTTGTGTCG AGAGCACTGC TCTCAGGCTT 
ATAACAGCAT TAGGTAGCTC AGAGGTACAA CCGCAGTTTA CACGCTTCCT TAGTGATCCC 
AAAACAGTGC TCTCAGCAGA ATCTGAAGAA CTGAACCGAG CCTTGATATT GACCTTGGCT 
AGAGCAACTC ATGTAACAGA TTTTTTTACA GGCTCTGATT CAATTCAGGG AACTTGGTGT 
AAAGACATAC TTCAGACCAT CATGAGTTTC ACTCCTCATA ATTGGGCTTC ACACACCCTG 
AGCTGTTTTC CAGGCCCACT ACAGGCATTC TTCAAACAAA ATAATGTGCC TCAGGAAAGC 
CGTTTTAATC TGAAAAAAAA TGTGGAGGAG GAGTATAGGA AGTGGAAGTC AATGAGCAAC 
GAAAACGACA TTATTACCCA CTTCTCTATG CAGGGCTCCC CTCCTCTCTT TCTTTGTCTT 
CTCTGGAAAA TGCTCTTGGA AACAGATCAT ATTAATCAGA TTGGCTATAG AGTATTAGAG 
AGAATTGGAG CCAGGGCCTT GGTAGCCCAT GTGAGGACAT TTGCAGATTT CCTGGTATAT 
GAGTTTTCTA CATCAGCAGG GGGTCAGCAA CTCAATAAAT GCATTGAAAT TCTTAATGAC 
ATGGTATGGA AGTATAACAT TGTTACACTG GACAGATTAA TTCTCTGCCT GGCCATGCGT 
AGTCACGAAG GAAATGAAGC CCAGGTTTGT TATTTCATAA TTCAGTTGCT GTTACTCAAA 
CCAAACGATT TTAGAAATCG AGTAAGTGAC TTTGTGAAGG AAAATTCCCC AGAGCACTGG 
TTACAGAATG ACTGGCACAC CAAGCACATG AATTATCACA AGAAATATCC AGAGAAGTTG 
TATTTTGAGG GCCTCGCGGA ACAGGTGGAT CCTCCTGTAC AGATCCAGTC TCCCTATCTG 
CCCATCTATT TTGGGAATGT GTGTCTTCGA TTCCTTCCAG TATTTGATAT AGTAATCCAC 
AGATTTTTAG AGTTGCTTCC GGTATCCAAA TCACTGGAGA CTCTACTGGA TCATCTAGGA 
GGCTTATATA AATTTCATGA TCGTCCAGTG ACTTATCTGT ATAACACTCT GCACTATTAT 
GAAATGCACC TGAGAGACCG CGCATTTCTC AAACGAAAAC TCGTCCATGC GATCATTGGC 
TCTCTGAAGG ATAATCGACC GCAGGGCTGG TGTCTAAGTG ACACTTACCT GAAATGCGCT 
ATGAATGCAC GAGAGGAAAA TCCTTGGGTT CCAGATGACA CCTACTATTG CAGATTGATT 
GGCAGACTAG TCGATACGAT GGCTGGCAAA TCTCCTGGTC CCTTTCCAAA CTGTGACTGG 
AGATTCAATG AGTTTCCCAA CCCAGCTGCC CATGCTCTCC ATGTTACTTG TGTGGAGCTC 
ATGGCCTTGG CAGTTTCAGG CAAAGAAGTT GGGAATGCCC TTCTAAATGT TGTCCTAAAA 
AGTCAGCCTT TAGTGCCAAG AGAGAACATT ACAGCATGGA TGAATGCAAT TGGTTTGATC 
ATCACTGCCC TACCAGAGCC ATATTGGATT GTTCTTCATG ATCGAATTGT GAGTGTCATC 
AGCAGCCCCA GCTTGACGTC TGAAACAGAG TGGGTTGGCT ATCCATTCCG CCTCTTTGAT 
TTCACTGCCT GTCATCAGTC CTACTCTGAG ATGAGTTGTA GCTATACGTT AGCTCTTGCA 
CATGCTGTGT GGCACCATTC TAGCATCGGA CAACTTTCTC TCATTCCAAA GTTTCTTACT 
GAAGTACTTC TTCCTATAGT GAAGACCGAA TTCCAGTTGC TTTATGTATA CCATCTTGTT 
GGACCATTTT TACAAAGATT TCAGCAAGAG AGAACTCGTT GTATGATAGA GATTGGTGTG 
GCGTTTTATG ACATGCTGCT GAATGTTGAC CAGTGTAGCA CCCATTTAAA TTACATGGAT 
CCCATCTGTG ACTTCCTCTA TCACATGAAG TATATGTTTA CTGGTGACAG CGTGAAAGAG 
CAAGTAGAGA AGATTATCTG TAACTTAAAA CCAGCTTTAA AACTTCGTCT TCGATTCATC 
ACACACATTA GCAAGATGGA GCCAGCTGCA GTGCCTCCAC AAGCCATGAA CAGTGGGTCT 
CCAGCACCTC AGTCTAATCA GGTGCCCGTG TCTTTACCAG TAACTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534559.1
CDS	187..4197
Translation	METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQVPVSLPVTQ

Target ORF information:

RefSeq Version	XM_011536257.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536257.2

ORF Insert Sequence:

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
CACAAGCAGC GCTGCCCTGT GCTGGAGGAC CAGTTGGTGG ATCTGGTTGT TTATGCCATG 
GAGCGATCTG AGACCGAGGA GAAGTTTGAC GATGGGGGAA CAAGCCAACT CCTGTGGCAG 
CATCTCTCAA GTCAGCTCAT TTTCTTTGTG CTTTTCCAGT TTGCAAGTTT TCCACATATG 
GTGCTTTCTC TTCATCAGAA GTTAGCAGGG CGAGGACTGA TTAAAGGCAG AGATCATCTT 
ATGTGGGTTC TCCTGCAATT CATTTCTGGA AGTATTCAGA AAAATGCACT AGCTGATTTT 
CTCCCTGTGA TGAAGCTCTT CGACTTGCTA TACCCAGAAA AAGAATATAT CCCAGTTCCT 
GATATTAACA AACCCCAGTC AACCCATGCC TTTGCAATGA CCTGTATTTG GATTCATCTC 
AATAGAAAAG CTCAAAATGA CAACTCCAAG CTACAGATTC CAATACCTCA TTCCCTAAGA 
CTTCACCATG AGTTCCTGCA GCAGAGTCTA AGAAATAAAA GTTTACAGAT GAATGACTAT 
AAGATTGCTC TATTGTGTAA TGCATACTCT ACAAATTCAG AATGTTTTAC ATTACCCATG 
GGAGCTCTGG TAGAAACTAT TTATGGAAAT GGAATTATGA GGATACCTCT CCCTGGAACA 
AACTGTATGG CTTCAGGATC TATTACCCCC TTACCTATGA ACCTCCTGGA TTCACTGACA 
GTTCATGCCA AAATGAGCCT TATTCACAGC ATTGCAACCA GGGTGATAAA ACTTGCTCAT 
GCAAAGTCCA GTGTGGCCTT GGCTCCAGCC CTAGTGGAAA CTTACAGTCG TTTATTGGTC 
TATATGGAAA TAGAGTCTTT GGGCATCAAA GGATTTATCA GTCAGCTTTT GCCAACTGTT 
TTCAAATCAC ATGCATGGGG GATCTTACAC ACACTCCTTG AGATGTTTAG CTACCGGATG 
CATCATATTC AGCCTCATTA CAGAGTTCAG CTCCTGAGTC ATCTTCATAC TTTGGCTGCA 
GTTGCACAAA CAAACCAGAA CCAGCTCCAT CTTTGTGTCG AGAGCACTGC TCTCAGGCTT 
ATAACAGCAT TAGGTAGCTC AGAGGTACAA CCGCAGTTTA CACGCTTCCT TAGTGATCCC 
AAAACAGTGC TCTCAGCAGA ATCTGAAGAA CTGAACCGAG CCTTGATATT GACCTTGGCT 
AGAGCAACTC ATGTAACAGA TTTTTTTACA GGCTCTGATT CAATTCAGGG AACTTGGTGT 
AAAGACATAC TTCAGACCAT CATGAGTTTC ACTCCTCATA ATTGGGCTTC ACACACCCTG 
AGCTGTTTTC CAGGCCCACT ACAGGCATTC TTCAAACAAA ATAATGTGCC TCAGGAAAGC 
CGTTTTAATC TGAAAAAAAA TGTGGAGGAG GAGTATAGGA AGTGGAAGTC AATGAGCAAC 
GAAAACGACA TTATTACCCA CTTCTCTATG CAGGGCTCCC CTCCTCTCTT TCTTTGTCTT 
CTCTGGAAAA TGCTCTTGGA AACAGATCAT ATTAATCAGA TTGGCTATAG AGTATTAGAG 
AGAATTGGAG CCAGGGCCTT GGTAGCCCAT GTGAGGACAT TTGCAGATTT CCTGGTATAT 
GAGTTTTCTA CATCAGCAGG GGGTCAGCAA CTCAATAAAT GCATTGAAAT TCTTAATGAC 
ATGGTATGGA AGTATAACAT TGTTACACTG GACAGATTAA TTCTCTGCCT GGCCATGCGT 
AGTCACGAAG GAAATGAAGC CCAGGTTTGT TATTTCATAA TTCAGTTGCT GTTACTCAAA 
CCAAACGATT TTAGAAATCG AGTAAGTGAC TTTGTGAAGG AAAATTCCCC AGAGCACTGG 
TTACAGAATG ACTGGCACAC CAAGCACATG AATTATCACA AGAAATATCC AGAGAAGTTG 
TATTTTGAGG GCCTCGCGGA ACAGGTGGAT CCTCCTGTAC AGATCCAGTC TCCCTATCTG 
CCCATCTATT TTGGGAATGT GTGTCTTCGA TTCCTTCCAG TATTTGATAT AGTAATCCAC 
AGATTTTTAG AGTTGCTTCC GGTATCCAAA TCACTGGAGA CTCTACTGGA TCATCTAGGA 
GGCTTATATA AATTTCATGA TCGTCCAGTG ACTTATCTGT ATAACACTCT GCACTATTAT 
GAAATGCACC TGAGAGACCG CGCATTTCTC AAACGAAAAC TCGTCCATGC GATCATTGGC 
TCTCTGAAGG ATAATCGACC GCAGGGCTGG TGTCTAAGTG ACACTTACCT GAAATGCGCT 
ATGAATGCAC GAGAGGAAAA TCCTTGGGTT CCAGATGACA CCTACTATTG CAGATTGATT 
GGCAGACTAG TCGATACGAT GGCTGGCAAA TCTCCTGGTC CCTTTCCAAA CTGTGACTGG 
AGATTCAATG AGTTTCCCAA CCCAGCTGCC CATGCTCTCC ATGTTACTTG TGTGGAGCTC 
ATGGCCTTGG CAGTTTCAGG CAAAGAAGTT GGGAATGCCC TTCTAAATGT TGTCCTAAAA 
AGTCAGCCTT TAGTGCCAAG AGAGAACATT ACAGCATGGA TGAATGCAAT TGGTTTGATC 
ATCACTGCCC TACCAGAGCC ATATTGGATT GTTCTTCATG ATCGAATTGT GAGTGTCATC 
AGCAGCCCCA GCTTGACGTC TGAAACAGAG TGGGTTGGCT ATCCATTCCG CCTCTTTGAT 
TTCACTGCCT GTCATCAGTC CTACTCTGAG ATGAGTTGTA GCTATACGTT AGCTCTTGCA 
CATGCTGTGT GGCACCATTC TAGCATCGGA CAACTTTCTC TCATTCCAAA GTTTCTTACT 
GAAGTACTTC TTCCTATAGT GAAGACCGAA TTCCAGTTGC TTTATGTATA CCATCTTGTT 
GGACCATTTT TACAAAGATT TCAGCAAGAG AGAACTCGTT GTATGATAGA GATTGGTGTG 
GCGTTTTATG ACATGCTGCT GAATGTTGAC CAGTGTAGCA CCCATTTAAA TTACATGGAT 
CCCATCTGTG ACTTCCTCTA TCACATGAAG TATATGTTTA CTGGTGACAG CGTGAAAGAG 
CAAGTAGAGA AGATTATCTG TAACTTAAAA CCAGCTTTAA AACTTCGTCT TCGATTCATC 
ACACACATTA GCAAGATGGA GCCAGCTGCA GTGCCTCCAC AAGCCATGAA CAGTGGGTCT 
CCAGCACCTC AGTCTAATCA GGTGCCCGTG TCTTTACCAG TAACTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED23 ( XM_011536257.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534559.1 Homo sapiens mediator complex subunit 23 (MED23), transcript variant X2, mRNA.

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CloneID	OHu62822
Clone ID Related Accession (Same CDS sequence)	XM_011536257.2 , XM_011536257.3
Accession Version	XM_011536257.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 23 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011536257.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
CACAAGCAGC GCTGCCCTGT GCTGGAGGAC CAGTTGGTGG ATCTGGTTGT TTATGCCATG 
GAGCGATCTG AGACCGAGGA GAAGTTTGAC GATGGGGGAA CAAGCCAACT CCTGTGGCAG 
CATCTCTCAA GTCAGCTCAT TTTCTTTGTG CTTTTCCAGT TTGCAAGTTT TCCACATATG 
GTGCTTTCTC TTCATCAGAA GTTAGCAGGG CGAGGACTGA TTAAAGGCAG AGATCATCTT 
ATGTGGGTTC TCCTGCAATT CATTTCTGGA AGTATTCAGA AAAATGCACT AGCTGATTTT 
CTCCCTGTGA TGAAGCTCTT CGACTTGCTA TACCCAGAAA AAGAATATAT CCCAGTTCCT 
GATATTAACA AACCCCAGTC AACCCATGCC TTTGCAATGA CCTGTATTTG GATTCATCTC 
AATAGAAAAG CTCAAAATGA CAACTCCAAG CTACAGATTC CAATACCTCA TTCCCTAAGA 
CTTCACCATG AGTTCCTGCA GCAGAGTCTA AGAAATAAAA GTTTACAGAT GAATGACTAT 
AAGATTGCTC TATTGTGTAA TGCATACTCT ACAAATTCAG AATGTTTTAC ATTACCCATG 
GGAGCTCTGG TAGAAACTAT TTATGGAAAT GGAATTATGA GGATACCTCT CCCTGGAACA 
AACTGTATGG CTTCAGGATC TATTACCCCC TTACCTATGA ACCTCCTGGA TTCACTGACA 
GTTCATGCCA AAATGAGCCT TATTCACAGC ATTGCAACCA GGGTGATAAA ACTTGCTCAT 
GCAAAGTCCA GTGTGGCCTT GGCTCCAGCC CTAGTGGAAA CTTACAGTCG TTTATTGGTC 
TATATGGAAA TAGAGTCTTT GGGCATCAAA GGATTTATCA GTCAGCTTTT GCCAACTGTT 
TTCAAATCAC ATGCATGGGG GATCTTACAC ACACTCCTTG AGATGTTTAG CTACCGGATG 
CATCATATTC AGCCTCATTA CAGAGTTCAG CTCCTGAGTC ATCTTCATAC TTTGGCTGCA 
GTTGCACAAA CAAACCAGAA CCAGCTCCAT CTTTGTGTCG AGAGCACTGC TCTCAGGCTT 
ATAACAGCAT TAGGTAGCTC AGAGGTACAA CCGCAGTTTA CACGCTTCCT TAGTGATCCC 
AAAACAGTGC TCTCAGCAGA ATCTGAAGAA CTGAACCGAG CCTTGATATT GACCTTGGCT 
AGAGCAACTC ATGTAACAGA TTTTTTTACA GGCTCTGATT CAATTCAGGG AACTTGGTGT 
AAAGACATAC TTCAGACCAT CATGAGTTTC ACTCCTCATA ATTGGGCTTC ACACACCCTG 
AGCTGTTTTC CAGGCCCACT ACAGGCATTC TTCAAACAAA ATAATGTGCC TCAGGAAAGC 
CGTTTTAATC TGAAAAAAAA TGTGGAGGAG GAGTATAGGA AGTGGAAGTC AATGAGCAAC 
GAAAACGACA TTATTACCCA CTTCTCTATG CAGGGCTCCC CTCCTCTCTT TCTTTGTCTT 
CTCTGGAAAA TGCTCTTGGA AACAGATCAT ATTAATCAGA TTGGCTATAG AGTATTAGAG 
AGAATTGGAG CCAGGGCCTT GGTAGCCCAT GTGAGGACAT TTGCAGATTT CCTGGTATAT 
GAGTTTTCTA CATCAGCAGG GGGTCAGCAA CTCAATAAAT GCATTGAAAT TCTTAATGAC 
ATGGTATGGA AGTATAACAT TGTTACACTG GACAGATTAA TTCTCTGCCT GGCCATGCGT 
AGTCACGAAG GAAATGAAGC CCAGGTTTGT TATTTCATAA TTCAGTTGCT GTTACTCAAA 
CCAAACGATT TTAGAAATCG AGTAAGTGAC TTTGTGAAGG AAAATTCCCC AGAGCACTGG 
TTACAGAATG ACTGGCACAC CAAGCACATG AATTATCACA AGAAATATCC AGAGAAGTTG 
TATTTTGAGG GCCTCGCGGA ACAGGTGGAT CCTCCTGTAC AGATCCAGTC TCCCTATCTG 
CCCATCTATT TTGGGAATGT GTGTCTTCGA TTCCTTCCAG TATTTGATAT AGTAATCCAC 
AGATTTTTAG AGTTGCTTCC GGTATCCAAA TCACTGGAGA CTCTACTGGA TCATCTAGGA 
GGCTTATATA AATTTCATGA TCGTCCAGTG ACTTATCTGT ATAACACTCT GCACTATTAT 
GAAATGCACC TGAGAGACCG CGCATTTCTC AAACGAAAAC TCGTCCATGC GATCATTGGC 
TCTCTGAAGG ATAATCGACC GCAGGGCTGG TGTCTAAGTG ACACTTACCT GAAATGCGCT 
ATGAATGCAC GAGAGGAAAA TCCTTGGGTT CCAGATGACA CCTACTATTG CAGATTGATT 
GGCAGACTAG TCGATACGAT GGCTGGCAAA TCTCCTGGTC CCTTTCCAAA CTGTGACTGG 
AGATTCAATG AGTTTCCCAA CCCAGCTGCC CATGCTCTCC ATGTTACTTG TGTGGAGCTC 
ATGGCCTTGG CAGTTTCAGG CAAAGAAGTT GGGAATGCCC TTCTAAATGT TGTCCTAAAA 
AGTCAGCCTT TAGTGCCAAG AGAGAACATT ACAGCATGGA TGAATGCAAT TGGTTTGATC 
ATCACTGCCC TACCAGAGCC ATATTGGATT GTTCTTCATG ATCGAATTGT GAGTGTCATC 
AGCAGCCCCA GCTTGACGTC TGAAACAGAG TGGGTTGGCT ATCCATTCCG CCTCTTTGAT 
TTCACTGCCT GTCATCAGTC CTACTCTGAG ATGAGTTGTA GCTATACGTT AGCTCTTGCA 
CATGCTGTGT GGCACCATTC TAGCATCGGA CAACTTTCTC TCATTCCAAA GTTTCTTACT 
GAAGTACTTC TTCCTATAGT GAAGACCGAA TTCCAGTTGC TTTATGTATA CCATCTTGTT 
GGACCATTTT TACAAAGATT TCAGCAAGAG AGAACTCGTT GTATGATAGA GATTGGTGTG 
GCGTTTTATG ACATGCTGCT GAATGTTGAC CAGTGTAGCA CCCATTTAAA TTACATGGAT 
CCCATCTGTG ACTTCCTCTA TCACATGAAG TATATGTTTA CTGGTGACAG CGTGAAAGAG 
CAAGTAGAGA AGATTATCTG TAACTTAAAA CCAGCTTTAA AACTTCGTCT TCGATTCATC 
ACACACATTA GCAAGATGGA GCCAGCTGCA GTGCCTCCAC AAGCCATGAA CAGTGGGTCT 
CCAGCACCTC AGTCTAATCA GGTGCCCGTG TCTTTACCAG TAACTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534559.1
CDS	183..4193
Translation	METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQVPVSLPVTQ

Target ORF information:

RefSeq Version	XM_011536257.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 23 (MED23), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536257.3

ORF Insert Sequence:

ATGGAGACGC AACTGCAGAG CATTTTCGAA GAGGTGGTGA AAACGGAAGT TATAGAAGAG 
GCTTTTCCTG GCATGTTTAT GGATACTCCT GAAGATGAGA AAACAAAACT AATTAGCTGT 
TTGGGGGCCT TCAGACAGTT TTGGGGTGGT CTTTCTCAGG AGTCTCATGA ACAGTGTATC 
CAGTGGATTG TTAAGTTTAT TCATGGTCAG CATAGTCCTA AAAGAATTTC TTTTCTTTAT 
GACTGCTTAG CAATGGCAGT TGAGACTGGT CTCCTTCCAC CCAGGCTGGT TTGTGAATCC 
CTGATAAACT CTGACACTCT TGAGTGGGAA AGAACACAGC TTTGGGCCTT AACATTTAAA 
CTGGTTCGGA AAATAATTGG GGGAGTGGAT TACAAGGGTG TTCGAGATCT CTTAAAAGTG 
ATTTTGGAGA AGATTTTGAC AATTCCTAAT ACAGTGAGCT CTGCTGTTGT ACAGCAGCTT 
CTGGCAGCAA GAGAGGTTAT AGCATATATC TTGGAAAGAA ATGCCTGCTT ATTACCAGCC 
TATTTTGCAG TCACTGAGAT CAGGAAACTG TATCCTGAAG GCAAACTTCC ACACTGGTTA 
CTTGGAAACC TAGTATCAGA CTTTGTGGAT ACCTTCAGGC CCACAGCAAG GATAAACTCC 
ATTTGTGGTC GCTGTAGTCT TCTGCCAGTT GTAAATAATT CGGGTGCCAT TTGTAATTCA 
TGGAAACTGG ATCCTGCTAC TCTTCGTTTT CCTTTGAAAG GCCTTTTGCC ATATGATAAG 
CACAAGCAGC GCTGCCCTGT GCTGGAGGAC CAGTTGGTGG ATCTGGTTGT TTATGCCATG 
GAGCGATCTG AGACCGAGGA GAAGTTTGAC GATGGGGGAA CAAGCCAACT CCTGTGGCAG 
CATCTCTCAA GTCAGCTCAT TTTCTTTGTG CTTTTCCAGT TTGCAAGTTT TCCACATATG 
GTGCTTTCTC TTCATCAGAA GTTAGCAGGG CGAGGACTGA TTAAAGGCAG AGATCATCTT 
ATGTGGGTTC TCCTGCAATT CATTTCTGGA AGTATTCAGA AAAATGCACT AGCTGATTTT 
CTCCCTGTGA TGAAGCTCTT CGACTTGCTA TACCCAGAAA AAGAATATAT CCCAGTTCCT 
GATATTAACA AACCCCAGTC AACCCATGCC TTTGCAATGA CCTGTATTTG GATTCATCTC 
AATAGAAAAG CTCAAAATGA CAACTCCAAG CTACAGATTC CAATACCTCA TTCCCTAAGA 
CTTCACCATG AGTTCCTGCA GCAGAGTCTA AGAAATAAAA GTTTACAGAT GAATGACTAT 
AAGATTGCTC TATTGTGTAA TGCATACTCT ACAAATTCAG AATGTTTTAC ATTACCCATG 
GGAGCTCTGG TAGAAACTAT TTATGGAAAT GGAATTATGA GGATACCTCT CCCTGGAACA 
AACTGTATGG CTTCAGGATC TATTACCCCC TTACCTATGA ACCTCCTGGA TTCACTGACA 
GTTCATGCCA AAATGAGCCT TATTCACAGC ATTGCAACCA GGGTGATAAA ACTTGCTCAT 
GCAAAGTCCA GTGTGGCCTT GGCTCCAGCC CTAGTGGAAA CTTACAGTCG TTTATTGGTC 
TATATGGAAA TAGAGTCTTT GGGCATCAAA GGATTTATCA GTCAGCTTTT GCCAACTGTT 
TTCAAATCAC ATGCATGGGG GATCTTACAC ACACTCCTTG AGATGTTTAG CTACCGGATG 
CATCATATTC AGCCTCATTA CAGAGTTCAG CTCCTGAGTC ATCTTCATAC TTTGGCTGCA 
GTTGCACAAA CAAACCAGAA CCAGCTCCAT CTTTGTGTCG AGAGCACTGC TCTCAGGCTT 
ATAACAGCAT TAGGTAGCTC AGAGGTACAA CCGCAGTTTA CACGCTTCCT TAGTGATCCC 
AAAACAGTGC TCTCAGCAGA ATCTGAAGAA CTGAACCGAG CCTTGATATT GACCTTGGCT 
AGAGCAACTC ATGTAACAGA TTTTTTTACA GGCTCTGATT CAATTCAGGG AACTTGGTGT 
AAAGACATAC TTCAGACCAT CATGAGTTTC ACTCCTCATA ATTGGGCTTC ACACACCCTG 
AGCTGTTTTC CAGGCCCACT ACAGGCATTC TTCAAACAAA ATAATGTGCC TCAGGAAAGC 
CGTTTTAATC TGAAAAAAAA TGTGGAGGAG GAGTATAGGA AGTGGAAGTC AATGAGCAAC 
GAAAACGACA TTATTACCCA CTTCTCTATG CAGGGCTCCC CTCCTCTCTT TCTTTGTCTT 
CTCTGGAAAA TGCTCTTGGA AACAGATCAT ATTAATCAGA TTGGCTATAG AGTATTAGAG 
AGAATTGGAG CCAGGGCCTT GGTAGCCCAT GTGAGGACAT TTGCAGATTT CCTGGTATAT 
GAGTTTTCTA CATCAGCAGG GGGTCAGCAA CTCAATAAAT GCATTGAAAT TCTTAATGAC 
ATGGTATGGA AGTATAACAT TGTTACACTG GACAGATTAA TTCTCTGCCT GGCCATGCGT 
AGTCACGAAG GAAATGAAGC CCAGGTTTGT TATTTCATAA TTCAGTTGCT GTTACTCAAA 
CCAAACGATT TTAGAAATCG AGTAAGTGAC TTTGTGAAGG AAAATTCCCC AGAGCACTGG 
TTACAGAATG ACTGGCACAC CAAGCACATG AATTATCACA AGAAATATCC AGAGAAGTTG 
TATTTTGAGG GCCTCGCGGA ACAGGTGGAT CCTCCTGTAC AGATCCAGTC TCCCTATCTG 
CCCATCTATT TTGGGAATGT GTGTCTTCGA TTCCTTCCAG TATTTGATAT AGTAATCCAC 
AGATTTTTAG AGTTGCTTCC GGTATCCAAA TCACTGGAGA CTCTACTGGA TCATCTAGGA 
GGCTTATATA AATTTCATGA TCGTCCAGTG ACTTATCTGT ATAACACTCT GCACTATTAT 
GAAATGCACC TGAGAGACCG CGCATTTCTC AAACGAAAAC TCGTCCATGC GATCATTGGC 
TCTCTGAAGG ATAATCGACC GCAGGGCTGG TGTCTAAGTG ACACTTACCT GAAATGCGCT 
ATGAATGCAC GAGAGGAAAA TCCTTGGGTT CCAGATGACA CCTACTATTG CAGATTGATT 
GGCAGACTAG TCGATACGAT GGCTGGCAAA TCTCCTGGTC CCTTTCCAAA CTGTGACTGG 
AGATTCAATG AGTTTCCCAA CCCAGCTGCC CATGCTCTCC ATGTTACTTG TGTGGAGCTC 
ATGGCCTTGG CAGTTTCAGG CAAAGAAGTT GGGAATGCCC TTCTAAATGT TGTCCTAAAA 
AGTCAGCCTT TAGTGCCAAG AGAGAACATT ACAGCATGGA TGAATGCAAT TGGTTTGATC 
ATCACTGCCC TACCAGAGCC ATATTGGATT GTTCTTCATG ATCGAATTGT GAGTGTCATC 
AGCAGCCCCA GCTTGACGTC TGAAACAGAG TGGGTTGGCT ATCCATTCCG CCTCTTTGAT 
TTCACTGCCT GTCATCAGTC CTACTCTGAG ATGAGTTGTA GCTATACGTT AGCTCTTGCA 
CATGCTGTGT GGCACCATTC TAGCATCGGA CAACTTTCTC TCATTCCAAA GTTTCTTACT 
GAAGTACTTC TTCCTATAGT GAAGACCGAA TTCCAGTTGC TTTATGTATA CCATCTTGTT 
GGACCATTTT TACAAAGATT TCAGCAAGAG AGAACTCGTT GTATGATAGA GATTGGTGTG 
GCGTTTTATG ACATGCTGCT GAATGTTGAC CAGTGTAGCA CCCATTTAAA TTACATGGAT 
CCCATCTGTG ACTTCCTCTA TCACATGAAG TATATGTTTA CTGGTGACAG CGTGAAAGAG 
CAAGTAGAGA AGATTATCTG TAACTTAAAA CCAGCTTTAA AACTTCGTCT TCGATTCATC 
ACACACATTA GCAAGATGGA GCCAGCTGCA GTGCCTCCAC AAGCCATGAA CAGTGGGTCT 
CCAGCACCTC AGTCTAATCA GGTGCCCGTG TCTTTACCAG TAACTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effect of l-arginine, asymmetric dimethylarginine, and symmetric dimethylarginine on ischemic heart disease risk: A Mendelian randomization study.
American heart journal18254-61(2016 Dec)
Au Yeung SL,Lin SL,Lam HS,Schooling CM

MED23-associated refractory epilepsy successfully treated with the ketogenic diet.
American journal of medical genetics. Part A170(9)2421-5(2016 Sep)
Lionel AC,Monfared N,Scherer SW,Marshall CR,Mercimek-Mahmutoglu S

A 7-Gene Signature Depicts the Biochemical Profile of Early Prefibrotic Myelofibrosis.
PloS one11(8)e0161570(2016)
Skov V,Burton M,Thomassen M,Stauffer Larsen T,Riley CH,Brinch Madelung A,Kj?r L,Bondo H,Stamp I,Ehinger M,Dahl-S?rensen R,Brochmann N,Nielsen K,Thiele J,Jensen MK,Weis Bjerrum O,Kruse TA,Hasselbalch HC

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MED23 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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