Nisch cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nisch
Entrez Gene ID	306255
Full Name	nischarin
Synonyms	I-1
General protein information	Preferred Names nischarin Names nischarin I-1 receptor candidate protein I1R IR1 imidazoline receptor 1 imidazoline receptor I-1 imidazoline-1 receptor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p16

mRNA and Protein(s)

mRNA	Protein	Name
XM_008771179.2	XP_008769401.2	nischarin isoform X3
XM_001058760.5	XP_001058760.5	nischarin isoform X1
XM_001057307.5	XP_001057307.5	nischarin isoform X1
XM_017600295.1	XP_017455784.1	nischarin isoform X2
NM_001376918.1	NP_001363847.1	nischarin
XM_008771056.2	XP_008769278.2	nischarin isoform X3
XM_017587539.1	XP_017443028.1	nischarin isoform X2

Pathways from BioSystems

Homologies

	NISCH	XP_001085527.2
Rattus norvegicus (Norway rat)	Nisch	XP_001057307.4
Pan troglodytes (chimpanzee)	NISCH	XP_001152331.2
Bos taurus (cattle)	NISCH	XP_002697110.2
Homo sapiens (human)	NISCH	NP_009115.2
Drosophila melanogaster (fruit fly)	CG11807	NP_001260990.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011394	XP_317926.4
Canis lupus familiaris (dog)	NISCH	XP_005633244.1

Gene Ontology
Bibliography

Related articles in PubMed

Inhibition of Nischarin Expression Promotes Neurite Outgrowth through Regulation of PAK Activity.
Ding Y, Li Y, Lu L, Zhang R, Zeng L, Wang L, Zhang X
PloS one10(12)e0144948(2015)

The expression pattern of Nischarin after lipopolysaccharides (LPS)-induced neuroinflammation in rats brain cortex.
Wu X, Xu W, Cui G, Yan Y, Wu X, Li L, Tan X, Wu Q, Gu X
Inflammation research : official journal of the European Histamine Research Society ... [et al.]62(11)929-40(2013 Nov)

Rac and Rab GTPases dual effector Nischarin regulates vesicle maturation to facilitate survival of intracellular bacteria.
Kuijl C, Pilli M, Alahari SK, Janssen H, Khoo PS, Ervin KE, Calero M, Jonnalagadda S, Scheller RH, Neefjes J, Junutula JR
The EMBO journal32(5)713-27(2013 Mar)

Nischarin is differentially expressed in rat brain and regulates neuronal migration.
Ding Y, Zhang R, Zhang K, Lv X, Chen Y, Li A, Wang L, Zhang X, Xia Q
PloS one8(1)e54563(2013)

I1 imidazoline receptor: novel potential cytoprotective target of TVP1022, the S-enantiomer of rasagiline.
Barac YD, Bar-Am O, Liani E, Amit T, Frolov L, Ovcharenko E, Angel I, Youdim MB, Binah O
PloS one7(11)e47890(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nischarin inhibits neurite outgrowth by blocking PAK1 activation in neurons.
Title: Inhibition of Nischarin Expression Promotes Neurite Outgrowth through Regulation of PAK Activity.

Nischarin might be involved in mediating the process of PI3K/PKB pathway-dependent neuronal apoptosis.
Title: The expression pattern of Nischarin after lipopolysaccharides (LPS)-induced neuroinflammation in rats brain cortex.

The results provide a preliminary survey of the distribution of Nischarin in different regions and cell types in the rat brain.
Title: Nischarin is differentially expressed in rat brain and regulates neuronal migration.

The I(1)imidazoline receptor represents a novel site of action for the cyto/cardio-protective efficacy of TVP1022.
Title: I1 imidazoline receptor: novel potential cytoprotective target of TVP1022, the S-enantiomer of rasagiline.

Taken together, these data support a role for IAA-RP in modulating synaptic transmission in the hippocampus through activation of I-Rs.
Title: Imidazoleacetic acid-ribotide induces depression of synaptic responses in hippocampus through activation of imidazoline receptors.

The following Nisch gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nisch cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa54532	NM_001376918.1 Latest version!	Rattus norvegicus nischarin (Nisch), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa54532	XM_008771179.2 Latest version!	Rattus norvegicus nischarin (Nisch), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa54532	XM_008771056.2 Latest version!	Rattus norvegicus nischarin (Nisch), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa54530	XM_001057307.5 Latest version!	Rattus norvegicus nischarin (Nisch), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa54530	XM_001058760.5 Latest version!	Rattus norvegicus nischarin (Nisch), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa54531	XM_017600295.1 Latest version!	Rattus norvegicus nischarin (Nisch), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa54531	XM_017587539.1 Latest version!	Rattus norvegicus nischarin (Nisch), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nisch ( NM_001376918.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001363847.1 Rattus norvegicus nischarin (Nisch), mRNA.

Price & Availability↑

CloneID	ORa54532
Clone ID Related Accession (Same CDS sequence)	XM_008771179.2 , NM_001376918.1 , XM_008771056.2
Accession Version	NM_001376918.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Rattus norvegicus(Norway rat)
Product	nischarin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095672.9. On or before Nov 27, 2019 this sequence version replaced XM_008771056.2, XM_008771179.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCTG CGACACTCAG CTTCGGCCCT GAGCGAGAGG CCGAGCCTGC CAAGGAGGCG 
CGCGTTGTGG GTTCCGAGCT CGTGGACACG TACACGGTAT ATGTCATACA GGTTACCGAT 
GGCAACCATG AGTGGACGAT CAAACACCGC TATAGTGATT TTCATGACCT CCATGAAAAG 
CTTGTTGCTG AGAGAAAAAT TGACAAAACT CTACTTCCAC CCAAAAAGAT AATTGGAAAG 
AACTCGAGAA GCTTGGTAGA AAAGAGGGAG AAAGACCTGG AGGTGTACCT CCAGACTCTC 
CTGAAGACCT TCCCTGATGT GGCTCCCAGA GTTCTGGCCC ACTTCCTGCA TTTTCACCTC 
TATGAAATAA ATGGTGTCAC TGCAGCACTG GCTGAAGAAC TCTTTGAAAA AGGAGAACAG 
CTTCTAGGAG CCGGTGAGGT CTTTGCCATC AGGCCCTTGC AGCTCTATGC TATCACAGAA 
CAGCTGCAGC AGGGAAAGCC TACCTGTGCC AGCGGTGATG CCAAGACTGA CCTTGGACAC 
ATCCTAGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAGA AGGACCTTTT 
GGGACCAGCA ATATTCGAGA GCAGCTCCTG CCCTTTGATC TTTCGATATT CAAGTCTCTT 
CACCAGGTGG AGATGAGTCA CTGTGATGCC AAGCATGTCC GAGGGCTGGT CACCTCCAAG 
CCAACACTGG CCACAATGAG CGTTAGATTC TCAGCAGCCT CAATGAAGGA AGTTCTTGTT 
CCTGAAGCCT CAGAATTTGA TGAGTGGGAG CCTGAAGGCA CAACCCTCGG AGGCCCTGTG 
ACTGCTGTCA TCCCAACATG GCAAGCACTG ACCACTCTAG ACCTGAGCCA CAACAGCATC 
TCTGAGATCG ACGAATCTGT GAAACTGATC CCAAAGATTG AGTATCTGGA CCTGAGTCAC 
AATGGAGTGC TAGTTGTGGA CAACCTGCAG CACCTATACA ACCTCGTGCA CCTTGACCTG 
TCCTACAACA AGCTCTCCTC CCTGGAAGGC GTACACACTA AACTGGGCAA TGTCAAAACC 
CTAAACCTGG CAGGCAACTT CTTAGAGCGT CTGAGCGGTC TGCACAAACT CTATTCCCTG 
GTTAATCTGG ACCTCAGAGA CAACCGGATT GAGCAGTTGG ATGAAGTCAA GAGCATAGGC 
AACCTGCCGT GTCTGGAGCA CGTGGCCCTG CTAAACAACC CTCTGAGCAT CATCCCTGAC 
TACCGGACCA AGGTGCTTTC CCAGTTTGGA GAACGAGCCT CTGAGATCTG TCTAGATGAT 
GTCGCAACCA CAGAGAAGGA ACTGGACACT GTGGAAGTGC TAAAGGCCAT TCAGAAAGCC 
AAAGACGTCA AGTCCAAACT GAGCAGCACA GAAAAGAAGG TTGGTGAGGA CTTCCGGCTC 
CCAACTGCTC CCTGCATCAG ACCCAGCAGC TCCCCTCCCA CTGCCGTTCC CACCTCAGCC 
TCCCTGCCTC AGCCTATCCT CTCCAACCAA GGCATCCTCG GAGATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363847.1
CDS	106..1656
Translation	MAAATLSFGPEREAEPAKEARVVGSELVDTYTVYVIQVTDGNHEWTIKHRYSDFHDLHEKLVAERKIDKTLLPPKKIIGKNSRSLVEKREKDLEVYLQTLLKTFPDVAPRVLAHFLHFHLYEINGVTAALAEELFEKGEQLLGAGEVFAIRPLQLYAITEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIREQLLPFDLSIFKSLHQVEMSHCDAKHVRGLVTSKPTLATMSVRFSAASMKEVLVPEASEFDEWEPEGTTLGGPVTAVIPTWQALTTLDLSHNSISEIDESVKLIPKIEYLDLSHNGVLVVDNLQHLYNLVHLDLSYNKLSSLEGVHTKLGNVKTLNLAGNFLERLSGLHKLYSLVNLDLRDNRIEQLDEVKSIGNLPCLEHVALLNNPLSIIPDYRTKVLSQFGERASEICLDDVATTEKELDTVEVLKAIQKAKDVKSKLSSTEKKVGEDFRLPTAPCIRPSSSPPTAVPTSASLPQPILSNQGILGDE

Target ORF information:

RefSeq Version	NM_001376918.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nischarin (Nisch), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376918.1

ORF Insert Sequence:

ATGGCGGCTG CGACACTCAG CTTCGGCCCT GAGCGAGAGG CCGAGCCTGC CAAGGAGGCG 
CGCGTTGTGG GTTCCGAGCT CGTGGACACG TACACGGTAT ATGTCATACA GGTTACCGAT 
GGCAACCATG AGTGGACGAT CAAACACCGC TATAGTGATT TTCATGACCT CCATGAAAAG 
CTTGTTGCTG AGAGAAAAAT TGACAAAACT CTACTTCCAC CCAAAAAGAT AATTGGAAAG 
AACTCGAGAA GCTTGGTAGA AAAGAGGGAG AAAGACCTGG AGGTGTACCT CCAGACTCTC 
CTGAAGACCT TCCCTGATGT GGCTCCCAGA GTTCTGGCCC ACTTCCTGCA TTTTCACCTC 
TATGAAATAA ATGGTGTCAC TGCAGCACTG GCTGAAGAAC TCTTTGAAAA AGGAGAACAG 
CTTCTAGGAG CCGGTGAGGT CTTTGCCATC AGGCCCTTGC AGCTCTATGC TATCACAGAA 
CAGCTGCAGC AGGGAAAGCC TACCTGTGCC AGCGGTGATG CCAAGACTGA CCTTGGACAC 
ATCCTAGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAGA AGGACCTTTT 
GGGACCAGCA ATATTCGAGA GCAGCTCCTG CCCTTTGATC TTTCGATATT CAAGTCTCTT 
CACCAGGTGG AGATGAGTCA CTGTGATGCC AAGCATGTCC GAGGGCTGGT CACCTCCAAG 
CCAACACTGG CCACAATGAG CGTTAGATTC TCAGCAGCCT CAATGAAGGA AGTTCTTGTT 
CCTGAAGCCT CAGAATTTGA TGAGTGGGAG CCTGAAGGCA CAACCCTCGG AGGCCCTGTG 
ACTGCTGTCA TCCCAACATG GCAAGCACTG ACCACTCTAG ACCTGAGCCA CAACAGCATC 
TCTGAGATCG ACGAATCTGT GAAACTGATC CCAAAGATTG AGTATCTGGA CCTGAGTCAC 
AATGGAGTGC TAGTTGTGGA CAACCTGCAG CACCTATACA ACCTCGTGCA CCTTGACCTG 
TCCTACAACA AGCTCTCCTC CCTGGAAGGC GTACACACTA AACTGGGCAA TGTCAAAACC 
CTAAACCTGG CAGGCAACTT CTTAGAGCGT CTGAGCGGTC TGCACAAACT CTATTCCCTG 
GTTAATCTGG ACCTCAGAGA CAACCGGATT GAGCAGTTGG ATGAAGTCAA GAGCATAGGC 
AACCTGCCGT GTCTGGAGCA CGTGGCCCTG CTAAACAACC CTCTGAGCAT CATCCCTGAC 
TACCGGACCA AGGTGCTTTC CCAGTTTGGA GAACGAGCCT CTGAGATCTG TCTAGATGAT 
GTCGCAACCA CAGAGAAGGA ACTGGACACT GTGGAAGTGC TAAAGGCCAT TCAGAAAGCC 
AAAGACGTCA AGTCCAAACT GAGCAGCACA GAAAAGAAGG TTGGTGAGGA CTTCCGGCTC 
CCAACTGCTC CCTGCATCAG ACCCAGCAGC TCCCCTCCCA CTGCCGTTCC CACCTCAGCC 
TCCCTGCCTC AGCCTATCCT CTCCAACCAA GGCATCCTCG GAGATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nisch ( XM_008771179.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008769401.2 Rattus norvegicus nischarin (Nisch), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa54532
Clone ID Related Accession (Same CDS sequence)	XM_008771179.2 , NM_001376918.1 , XM_008771056.2
Accession Version	XM_008771179.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nischarin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000084.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008771179.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGACACTCAG CTTCGGCCCT GAGCGAGAGG CCGAGCCTGC CAAGGAGGCG 
CGCGTTGTGG GTTCCGAGCT CGTGGACACG TACACGGTAT ATGTCATACA GGTTACCGAT 
GGCAACCATG AGTGGACGAT CAAACACCGC TATAGTGATT TTCATGACCT CCATGAAAAG 
CTTGTTGCTG AGAGAAAAAT TGACAAAACT CTACTTCCAC CCAAAAAGAT AATTGGAAAG 
AACTCGAGAA GCTTGGTAGA AAAGAGGGAG AAAGACCTGG AGGTGTACCT CCAGACTCTC 
CTGAAGACCT TCCCTGATGT GGCTCCCAGA GTTCTGGCCC ACTTCCTGCA TTTTCACCTC 
TATGAAATAA ATGGTGTCAC TGCAGCACTG GCTGAAGAAC TCTTTGAAAA AGGAGAACAG 
CTTCTAGGAG CCGGTGAGGT CTTTGCCATC AGGCCCTTGC AGCTCTATGC TATCACAGAA 
CAGCTGCAGC AGGGAAAGCC TACCTGTGCC AGCGGTGATG CCAAGACTGA CCTTGGACAC 
ATCCTAGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAGA AGGACCTTTT 
GGGACCAGCA ATATTCGAGA GCAGCTCCTG CCCTTTGATC TTTCGATATT CAAGTCTCTT 
CACCAGGTGG AGATGAGTCA CTGTGATGCC AAGCATGTCC GAGGGCTGGT CACCTCCAAG 
CCAACACTGG CCACAATGAG CGTTAGATTC TCAGCAGCCT CAATGAAGGA AGTTCTTGTT 
CCTGAAGCCT CAGAATTTGA TGAGTGGGAG CCTGAAGGCA CAACCCTCGG AGGCCCTGTG 
ACTGCTGTCA TCCCAACATG GCAAGCACTG ACCACTCTAG ACCTGAGCCA CAACAGCATC 
TCTGAGATCG ACGAATCTGT GAAACTGATC CCAAAGATTG AGTATCTGGA CCTGAGTCAC 
AATGGAGTGC TAGTTGTGGA CAACCTGCAG CACCTATACA ACCTCGTGCA CCTTGACCTG 
TCCTACAACA AGCTCTCCTC CCTGGAAGGC GTACACACTA AACTGGGCAA TGTCAAAACC 
CTAAACCTGG CAGGCAACTT CTTAGAGCGT CTGAGCGGTC TGCACAAACT CTATTCCCTG 
GTTAATCTGG ACCTCAGAGA CAACCGGATT GAGCAGTTGG ATGAAGTCAA GAGCATAGGC 
AACCTGCCGT GTCTGGAGCA CGTGGCCCTG CTAAACAACC CTCTGAGCAT CATCCCTGAC 
TACCGGACCA AGGTGCTTTC CCAGTTTGGA GAACGAGCCT CTGAGATCTG TCTAGATGAT 
GTCGCAACCA CAGAGAAGGA ACTGGACACT GTGGAAGTGC TAAAGGCCAT TCAGAAAGCC 
AAAGACGTCA AGTCCAAACT GAGCAGCACA GAAAAGAAGG TTGGTGAGGA CTTCCGGCTC 
CCAACTGCTC CCTGCATCAG ACCCAGCAGC TCCCCTCCCA CTGCCGTTCC CACCTCAGCC 
TCCCTGCCTC AGCCTATCCT CTCCAACCAA GGCATCCTCG GAGATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008769401.2
CDS	108..1658
Translation	MAAATLSFGPEREAEPAKEARVVGSELVDTYTVYVIQVTDGNHEWTIKHRYSDFHDLHEKLVAERKIDKTLLPPKKIIGKNSRSLVEKREKDLEVYLQTLLKTFPDVAPRVLAHFLHFHLYEINGVTAALAEELFEKGEQLLGAGEVFAIRPLQLYAITEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIREQLLPFDLSIFKSLHQVEMSHCDAKHVRGLVTSKPTLATMSVRFSAASMKEVLVPEASEFDEWEPEGTTLGGPVTAVIPTWQALTTLDLSHNSISEIDESVKLIPKIEYLDLSHNGVLVVDNLQHLYNLVHLDLSYNKLSSLEGVHTKLGNVKTLNLAGNFLERLSGLHKLYSLVNLDLRDNRIEQLDEVKSIGNLPCLEHVALLNNPLSIIPDYRTKVLSQFGERASEICLDDVATTEKELDTVEVLKAIQKAKDVKSKLSSTEKKVGEDFRLPTAPCIRPSSSPPTAVPTSASLPQPILSNQGILGDE

Target ORF information:

RefSeq Version	XM_008771179.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nischarin (Nisch), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008771179.2

ORF Insert Sequence:

ATGGCGGCTG CGACACTCAG CTTCGGCCCT GAGCGAGAGG CCGAGCCTGC CAAGGAGGCG 
CGCGTTGTGG GTTCCGAGCT CGTGGACACG TACACGGTAT ATGTCATACA GGTTACCGAT 
GGCAACCATG AGTGGACGAT CAAACACCGC TATAGTGATT TTCATGACCT CCATGAAAAG 
CTTGTTGCTG AGAGAAAAAT TGACAAAACT CTACTTCCAC CCAAAAAGAT AATTGGAAAG 
AACTCGAGAA GCTTGGTAGA AAAGAGGGAG AAAGACCTGG AGGTGTACCT CCAGACTCTC 
CTGAAGACCT TCCCTGATGT GGCTCCCAGA GTTCTGGCCC ACTTCCTGCA TTTTCACCTC 
TATGAAATAA ATGGTGTCAC TGCAGCACTG GCTGAAGAAC TCTTTGAAAA AGGAGAACAG 
CTTCTAGGAG CCGGTGAGGT CTTTGCCATC AGGCCCTTGC AGCTCTATGC TATCACAGAA 
CAGCTGCAGC AGGGAAAGCC TACCTGTGCC AGCGGTGATG CCAAGACTGA CCTTGGACAC 
ATCCTAGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAGA AGGACCTTTT 
GGGACCAGCA ATATTCGAGA GCAGCTCCTG CCCTTTGATC TTTCGATATT CAAGTCTCTT 
CACCAGGTGG AGATGAGTCA CTGTGATGCC AAGCATGTCC GAGGGCTGGT CACCTCCAAG 
CCAACACTGG CCACAATGAG CGTTAGATTC TCAGCAGCCT CAATGAAGGA AGTTCTTGTT 
CCTGAAGCCT CAGAATTTGA TGAGTGGGAG CCTGAAGGCA CAACCCTCGG AGGCCCTGTG 
ACTGCTGTCA TCCCAACATG GCAAGCACTG ACCACTCTAG ACCTGAGCCA CAACAGCATC 
TCTGAGATCG ACGAATCTGT GAAACTGATC CCAAAGATTG AGTATCTGGA CCTGAGTCAC 
AATGGAGTGC TAGTTGTGGA CAACCTGCAG CACCTATACA ACCTCGTGCA CCTTGACCTG 
TCCTACAACA AGCTCTCCTC CCTGGAAGGC GTACACACTA AACTGGGCAA TGTCAAAACC 
CTAAACCTGG CAGGCAACTT CTTAGAGCGT CTGAGCGGTC TGCACAAACT CTATTCCCTG 
GTTAATCTGG ACCTCAGAGA CAACCGGATT GAGCAGTTGG ATGAAGTCAA GAGCATAGGC 
AACCTGCCGT GTCTGGAGCA CGTGGCCCTG CTAAACAACC CTCTGAGCAT CATCCCTGAC 
TACCGGACCA AGGTGCTTTC CCAGTTTGGA GAACGAGCCT CTGAGATCTG TCTAGATGAT 
GTCGCAACCA CAGAGAAGGA ACTGGACACT GTGGAAGTGC TAAAGGCCAT TCAGAAAGCC 
AAAGACGTCA AGTCCAAACT GAGCAGCACA GAAAAGAAGG TTGGTGAGGA CTTCCGGCTC 
CCAACTGCTC CCTGCATCAG ACCCAGCAGC TCCCCTCCCA CTGCCGTTCC CACCTCAGCC 
TCCCTGCCTC AGCCTATCCT CTCCAACCAA GGCATCCTCG GAGATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nisch ( XM_008771056.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008769278.2 Rattus norvegicus nischarin (Nisch), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa54532
Clone ID Related Accession (Same CDS sequence)	XM_008771179.2 , NM_001376918.1 , XM_008771056.2
Accession Version	XM_008771056.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nischarin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008771056.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGACACTCAG CTTCGGCCCT GAGCGAGAGG CCGAGCCTGC CAAGGAGGCG 
CGCGTTGTGG GTTCCGAGCT CGTGGACACG TACACGGTAT ATGTCATACA GGTTACCGAT 
GGCAACCATG AGTGGACGAT CAAACACCGC TATAGTGATT TTCATGACCT CCATGAAAAG 
CTTGTTGCTG AGAGAAAAAT TGACAAAACT CTACTTCCAC CCAAAAAGAT AATTGGAAAG 
AACTCGAGAA GCTTGGTAGA AAAGAGGGAG AAAGACCTGG AGGTGTACCT CCAGACTCTC 
CTGAAGACCT TCCCTGATGT GGCTCCCAGA GTTCTGGCCC ACTTCCTGCA TTTTCACCTC 
TATGAAATAA ATGGTGTCAC TGCAGCACTG GCTGAAGAAC TCTTTGAAAA AGGAGAACAG 
CTTCTAGGAG CCGGTGAGGT CTTTGCCATC AGGCCCTTGC AGCTCTATGC TATCACAGAA 
CAGCTGCAGC AGGGAAAGCC TACCTGTGCC AGCGGTGATG CCAAGACTGA CCTTGGACAC 
ATCCTAGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAGA AGGACCTTTT 
GGGACCAGCA ATATTCGAGA GCAGCTCCTG CCCTTTGATC TTTCGATATT CAAGTCTCTT 
CACCAGGTGG AGATGAGTCA CTGTGATGCC AAGCATGTCC GAGGGCTGGT CACCTCCAAG 
CCAACACTGG CCACAATGAG CGTTAGATTC TCAGCAGCCT CAATGAAGGA AGTTCTTGTT 
CCTGAAGCCT CAGAATTTGA TGAGTGGGAG CCTGAAGGCA CAACCCTCGG AGGCCCTGTG 
ACTGCTGTCA TCCCAACATG GCAAGCACTG ACCACTCTAG ACCTGAGCCA CAACAGCATC 
TCTGAGATCG ACGAATCTGT GAAACTGATC CCAAAGATTG AGTATCTGGA CCTGAGTCAC 
AATGGAGTGC TAGTTGTGGA CAACCTGCAG CACCTATACA ACCTCGTGCA CCTTGACCTG 
TCCTACAACA AGCTCTCCTC CCTGGAAGGC GTACACACTA AACTGGGCAA TGTCAAAACC 
CTAAACCTGG CAGGCAACTT CTTAGAGCGT CTGAGCGGTC TGCACAAACT CTATTCCCTG 
GTTAATCTGG ACCTCAGAGA CAACCGGATT GAGCAGTTGG ATGAAGTCAA GAGCATAGGC 
AACCTGCCGT GTCTGGAGCA CGTGGCCCTG CTAAACAACC CTCTGAGCAT CATCCCTGAC 
TACCGGACCA AGGTGCTTTC CCAGTTTGGA GAACGAGCCT CTGAGATCTG TCTAGATGAT 
GTCGCAACCA CAGAGAAGGA ACTGGACACT GTGGAAGTGC TAAAGGCCAT TCAGAAAGCC 
AAAGACGTCA AGTCCAAACT GAGCAGCACA GAAAAGAAGG TTGGTGAGGA CTTCCGGCTC 
CCAACTGCTC CCTGCATCAG ACCCAGCAGC TCCCCTCCCA CTGCCGTTCC CACCTCAGCC 
TCCCTGCCTC AGCCTATCCT CTCCAACCAA GGCATCCTCG GAGATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008769278.2
CDS	106..1656
Translation	MAAATLSFGPEREAEPAKEARVVGSELVDTYTVYVIQVTDGNHEWTIKHRYSDFHDLHEKLVAERKIDKTLLPPKKIIGKNSRSLVEKREKDLEVYLQTLLKTFPDVAPRVLAHFLHFHLYEINGVTAALAEELFEKGEQLLGAGEVFAIRPLQLYAITEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIREQLLPFDLSIFKSLHQVEMSHCDAKHVRGLVTSKPTLATMSVRFSAASMKEVLVPEASEFDEWEPEGTTLGGPVTAVIPTWQALTTLDLSHNSISEIDESVKLIPKIEYLDLSHNGVLVVDNLQHLYNLVHLDLSYNKLSSLEGVHTKLGNVKTLNLAGNFLERLSGLHKLYSLVNLDLRDNRIEQLDEVKSIGNLPCLEHVALLNNPLSIIPDYRTKVLSQFGERASEICLDDVATTEKELDTVEVLKAIQKAKDVKSKLSSTEKKVGEDFRLPTAPCIRPSSSPPTAVPTSASLPQPILSNQGILGDE

Target ORF information:

RefSeq Version	XM_008771056.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nischarin (Nisch), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008771056.2

ORF Insert Sequence:

ATGGCGGCTG CGACACTCAG CTTCGGCCCT GAGCGAGAGG CCGAGCCTGC CAAGGAGGCG 
CGCGTTGTGG GTTCCGAGCT CGTGGACACG TACACGGTAT ATGTCATACA GGTTACCGAT 
GGCAACCATG AGTGGACGAT CAAACACCGC TATAGTGATT TTCATGACCT CCATGAAAAG 
CTTGTTGCTG AGAGAAAAAT TGACAAAACT CTACTTCCAC CCAAAAAGAT AATTGGAAAG 
AACTCGAGAA GCTTGGTAGA AAAGAGGGAG AAAGACCTGG AGGTGTACCT CCAGACTCTC 
CTGAAGACCT TCCCTGATGT GGCTCCCAGA GTTCTGGCCC ACTTCCTGCA TTTTCACCTC 
TATGAAATAA ATGGTGTCAC TGCAGCACTG GCTGAAGAAC TCTTTGAAAA AGGAGAACAG 
CTTCTAGGAG CCGGTGAGGT CTTTGCCATC AGGCCCTTGC AGCTCTATGC TATCACAGAA 
CAGCTGCAGC AGGGAAAGCC TACCTGTGCC AGCGGTGATG CCAAGACTGA CCTTGGACAC 
ATCCTAGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAGA AGGACCTTTT 
GGGACCAGCA ATATTCGAGA GCAGCTCCTG CCCTTTGATC TTTCGATATT CAAGTCTCTT 
CACCAGGTGG AGATGAGTCA CTGTGATGCC AAGCATGTCC GAGGGCTGGT CACCTCCAAG 
CCAACACTGG CCACAATGAG CGTTAGATTC TCAGCAGCCT CAATGAAGGA AGTTCTTGTT 
CCTGAAGCCT CAGAATTTGA TGAGTGGGAG CCTGAAGGCA CAACCCTCGG AGGCCCTGTG 
ACTGCTGTCA TCCCAACATG GCAAGCACTG ACCACTCTAG ACCTGAGCCA CAACAGCATC 
TCTGAGATCG ACGAATCTGT GAAACTGATC CCAAAGATTG AGTATCTGGA CCTGAGTCAC 
AATGGAGTGC TAGTTGTGGA CAACCTGCAG CACCTATACA ACCTCGTGCA CCTTGACCTG 
TCCTACAACA AGCTCTCCTC CCTGGAAGGC GTACACACTA AACTGGGCAA TGTCAAAACC 
CTAAACCTGG CAGGCAACTT CTTAGAGCGT CTGAGCGGTC TGCACAAACT CTATTCCCTG 
GTTAATCTGG ACCTCAGAGA CAACCGGATT GAGCAGTTGG ATGAAGTCAA GAGCATAGGC 
AACCTGCCGT GTCTGGAGCA CGTGGCCCTG CTAAACAACC CTCTGAGCAT CATCCCTGAC 
TACCGGACCA AGGTGCTTTC CCAGTTTGGA GAACGAGCCT CTGAGATCTG TCTAGATGAT 
GTCGCAACCA CAGAGAAGGA ACTGGACACT GTGGAAGTGC TAAAGGCCAT TCAGAAAGCC 
AAAGACGTCA AGTCCAAACT GAGCAGCACA GAAAAGAAGG TTGGTGAGGA CTTCCGGCTC 
CCAACTGCTC CCTGCATCAG ACCCAGCAGC TCCCCTCCCA CTGCCGTTCC CACCTCAGCC 
TCCCTGCCTC AGCCTATCCT CTCCAACCAA GGCATCCTCG GAGATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nisch ( XM_001058760.5 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_001058760.5 Rattus norvegicus nischarin (Nisch), transcript variant X1, mRNA.

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CloneID	ORa54530
Clone ID Related Accession (Same CDS sequence)	XM_001058760.5 , XM_001057307.5
Accession Version	XM_001058760.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nischarin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000084.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_001058760.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGACACTCAG CTTCGGCCCT GAGCGAGAGG CCGAGCCTGC CAAGGAGGCG 
CGCGTTGTGG GTTCCGAGCT CGTGGACACG TACACGGTAT ATGTCATACA GGTTACCGAT 
GGCAACCATG AGTGGACGAT CAAACACCGC TATAGTGATT TTCATGACCT CCATGAAAAG 
CTTGTTGCTG AGAGAAAAAT TGACAAAACT CTACTTCCAC CCAAAAAGAT AATTGGAAAG 
AACTCGAGAA GCTTGGTAGA AAAGAGGGAG AAAGACCTGG AGGTGTACCT CCAGACTCTC 
CTGAAGACCT TCCCTGATGT GGCTCCCAGA GTTCTGGCCC ACTTCCTGCA TTTTCACCTC 
TATGAAATAA ATGGTGTCAC TGCAGCACTG GCTGAAGAAC TCTTTGAAAA AGGAGAACAG 
CTTCTAGGAG CCGGTGAGGT CTTTGCCATC AGGCCCTTGC AGCTCTATGC TATCACAGAA 
CAGCTGCAGC AGGGAAAGCC TACCTGTGCC AGCGGTGATG CCAAGACTGA CCTTGGACAC 
ATCCTAGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAGA AGGACCTTTT 
GGGACCAGCA ATATTCGAGA GCAGCTCCTG CCCTTTGATC TTTCGATATT CAAGTCTCTT 
CACCAGGTGG AGATGAGTCA CTGTGATGCC AAGCATGTCC GAGGGCTGGT CACCTCCAAG 
CCAACACTGG CCACAATGAG CGTTAGATTC TCAGCAGCCT CAATGAAGGA AGTTCTTGTT 
CCTGAAGCCT CAGAATTTGA TGAGTGGGAG CCTGAAGGCA CAACCCTCGG AGGCCCTGTG 
ACTGCTGTCA TCCCAACATG GCAAGCACTG ACCACTCTAG ACCTGAGCCA CAACAGCATC 
TCTGAGATCG ACGAATCTGT GAAACTGATC CCAAAGATTG AGTATCTGGA CCTGAGTCAC 
AATGGAGTGC TAGTTGTGGA CAACCTGCAG CACCTATACA ACCTCGTGCA CCTTGACCTG 
TCCTACAACA AGCTCTCCTC CCTGGAAGGC GTACACACTA AACTGGGCAA TGTCAAAACC 
CTAAACCTGG CAGGCAACTT CTTAGAGCGT CTGAGCGGTC TGCACAAACT CTATTCCCTG 
GTTAATCTGG ACCTCAGAGA CAACCGGATT GAGCAGTTGG ATGAAGTCAA GAGCATAGGC 
AACCTGCCGT GTCTGGAGCA CGTGGCCCTG CTAAACAACC CTCTGAGCAT CATCCCTGAC 
TACCGGACCA AGGTGCTTTC CCAGTTTGGA GAACGAGCCT CTGAGATCTG TCTAGATGAT 
GTCGCAACCA CAGAGAAGGA ACTGGACACT GTGGAAGTGC TAAAGGCCAT TCAGAAAGCC 
AAAGACGTCA AGTCCAAACT GAGCAGCACA GAAAAGAAGG TTGGTGAGGA CTTCCGGCTC 
CCAACTGCTC CCTGCATCAG ACCCAGCAGC TCCCCTCCCA CTGCCGTTCC CACCTCAGCC 
TCCCTGCCTC AGCCTATCCT CTCCAACCAA GGTATCATGT TCGTACAGGA AGAGGCCCTG 
GCCAGCAGCC TCTCATCCAC GGATAGCCTG CCACCTGATG ACCGGCCCAT TGCCCAAGCC 
TGCTCTAACT CCATGGGATC TCTCCCCACA GGACAGGTGG CCGCTGAAGA CTTAAGGGAC 
TTGCCAGGAG CCGTTGGCGG TGTGAGCCCA GATCATGCAG AACCAGAGGT TCAGGTGGTG 
CCTGGGTCCG GCCAGATCAT CTTCCTGCCC TTCACTTGCA TTGGCTACAC AGCCACCAAC 
CAGGACTTTA TCCAGCGACT CAGCACACTG ATCCGCCAGG CCATTGAACG GCAACTCCCT 
GCCTGGATTG AGGCTGCCAA CCAGCGAGAG GAAGCCCATG GTGAACAGGG GGAGGAAGAG 
GAGGAGGAGG AGGAAGAGGA TGTCGCTGAG AGCCGCTACT TTGAAATGGG ACCCCCAGAC 
GCAGAGGAAG AGGAGGGGAG TGGCCAGGGA GAAGAGGATG AGGAAGACGA GGACGAGGAG 
GCCGAGGAGG AGCGCCTGGC TCTGGAGTGG GCCCTGGGCG CTGATGAGGA CTTCCTGCTG 
GAGCACATCC GCATCCTCAA GGTGCTGTGG TGCTTCCTGA TCCACGTGCA AGGCAGCATC 
CGCCAGTTCG CTGCCTGCCT TGTGCTCACC GACTTCGGCA TCGCTGTCTT TGAGATCCCA 
CACCAAGAGT CCCGAGGCAG CAGCCAGCAC ATCCTCTCAT CCCTGCGCTT CGTCTTCTGC 
TTCCCGCACG GCGACCTCAC CGAGTTTGGC TTCCTCATGC CGGAGCTGTG TCTGGTGCTC 
AAGGTGCGGC ACAGTGAGAA CACGCTCTTC ATCATCTCAG ACGCTGCCAA CTTACACGAG 
TTCCATGCTG ATCTGCGCTC CTGCTTTGCG CCACAGCACA TGGCCATGCT GTGCAGCCCC 
ATCCTCTACG GCAGCCACAC CAGCCTGCAA GAGTTCTTGC GCCAGCTGCT CACCTTCTAC 
AAGGTGGCCG GTGGCTCTCA GGAGCGCAGC CAGGGCTGCT TCCCTGTCTA CCTGGTGTAC 
AGCGACAAGC GCATGGTGCA GACGGCTGCC GGGGACTACT CAGGCAACAT CGAGTGGGCC 
AGCTGCACGC TGTGCTCCGC AGTGCGGCGT TCCTGCTGCG CACCCTCTGA GGCCGTCAAG 
TCTGCCGCCA TCCCCTACTG GCTGCTGCTC ACCTCCCAGC ATCTCAACGT CATCAAGGCC 
GACTTCAACC CCATGCCCAG TCGTGGCACC CACAACTGTC GCAACCGCAA CAGCTTCAAG 
CTTAGCCGCG TCCCGCTCTC CACCGTGCTG CTGGACCCCA CTCGCAGCTG CACCCAGCCA 
CGGGGAGCCT TCGCCGATGG CCATGTGCTC GAGCTGCTTG TTGGCTACCG CTTTGTTACC 
GCCATCTTTG TGCTGCCCCA CGAGAAATTC CACTTCCTGC GAGTCTACAA TCAGCTCCGC 
GCCTCACTGA AGGACCTGAA GACTGTGGTC ATCGCCAAGA ATCCTTCCGC TAGGCCGAGA 
ACCCAAGGCC CCCTCGCGGG TGGCCAGCCT GCCAAAAGCA GGGTCAGTGC TGAGCAGCGG 
CTGCAGGAGA CCCCAGCAGA AGCTCCCGCT CCAGTTCCGC CTCCAGCTGC AGTCCCACCA 
ACAGCTTCAG CCCCAGCCGC AACAGAGGCT GCAGCTCCAG CAGGGGCTCC AGCCGCAGCC 
GCATCTGCAG CAGAGGCTCC AGCTGTAGCC GCATCTGCAG CAGGGGCTCC AGCCGCAGCA 
GAGACTCCAG CCGCAGCCGA ATCTGCAACA GAGGCTCCAG CCGCAGCTGA ATCTGCAGCA 
GAGACTCCAG CCACAGCCGA ATCTGCAGCA GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA 
GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA GAGGCTCCAG CCGCTGCCGA ATCTGCAGCA 
GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA GAGGCTCCAG CAGCGGCAGA GGCTTCAGCT 
CCAGCGGGGG CTCCGGCTCC AGCGGGGGCT CCGGCTCCAG CAGGGGCTCC GGCAGGGGCT 
CAGGCTCCGG CTCCAGCGGG GGCTCCGGCG GGGGCTCCGG CAGGGGCTCA GGCTCTGGCA 
GGGGCTCAGG CTCCGGCTCC AGCGGGGGCT CCGGCAGGGG CTCAGGCTCC AGTGGAGGCC 
CAAGCAGAGG TCCCTGCTCA GTATCCAAGT GAGCGCCTAA TCCAGTCCAC GTCTGAAGAG 
AATCAGATCC CTTCTCACTT GCCAGTCTGC CCATCACTCC AGCACATCGC CCGTCTTCGG 
GGGCGCGCCA TCATTGACCT CTTCCACAGC AGCATTGCCG AGGTTGAAAA CGAGGAGCTA 
AGGCACCTCC TGTGGTCGTC AGTGGTGTTC TACCAGACCC CGGGGCTGGA AGTGACCGCC 
TGTGTGCTGC TGTCCACCAA GGCTGTGTAC TTCATACTGC ATGATGGCCT CCGCCGGTAC 
TTCTCTGAAC CGCTGCAGGA TTTCTGGCAC CAGAAAAACA CTGACTATAA CAACAGTCCT 
TTCCACATCT CTCAGTGCTT TGTGCTCAAA CTCAGTGACC TGCAGTCAGT CAACGTCGGC 
CTTTTCGATC AGTACTTCCG GCTGACGGGC TCTTCCCCGA CGCAGGTGGT CACATGCTTG 
ACTCGAGACA GCTACCTGAC ACACTGCTTC CTGCAGCACC TTATGCTAGT GCTGTCCTCC 
CTGGAGCGCA CACCTTCACC CGAGCCTATT GACAAGGACT TCTACTCAGA ATTTGGGGAC 
AAGAATACAG GGAAAATGGA GAACTATGAG CTGATCCATT CCAGCCGCGT CAAGTTCACC 
TACCCCAGTG AGGAAGAGGT CGGGGACCTG ACCTATGTTG TGGCACAGAA GATGGCTGAC 
CCTGCCAAGA ATCCAGCCCT CAGCATCTTA TTGTACATCC AGGCCTTCCA GGTGATCACA 
CCCCAGCTTG GGCGGGGCAG GGGCCCACTA CGCCCTAAGA CACTCCTACT GACCAGTGCT 
GAGATCTTTC TCCTGGACGA GGACTACATC CACTATCCAT TGCCTGAATT TGCCAAAGAG 
CCACCACAGA GGGACAGATA CCGGCTAGAC GATGGCCGCC GTGTCCGGGA TTTAGACCGG 
GTGCTCATGG GCTACAATCC CTACCCACAG GCCCTCACTC TTGTTTTTGA TGACACGCAG 
GGCCACGACC TCATGGGGAG TGTCACCCTG GACCACTTCG GGGAGATGCC GGGTGGCCCT 
GGTAGGGCTG GGCAGGGCCG GGAGGTGCAG TGGCAGGTGT TTGTGCCCAG TGCTGAGAGC 
CGAGAAAAGC TGATCTCACT GCTCGCACGG CAGTGGGAAG CTCTCTGTGG CAGGGAGCTG 
CCTGTGGAGC TCACTGGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001058760.5
CDS	112..5052
Translation	MAAATLSFGPEREAEPAKEARVVGSELVDTYTVYVIQVTDGNHEWTIKHRYSDFHDLHEKLVAERKIDKTLLPPKKIIGKNSRSLVEKREKDLEVYLQTLLKTFPDVAPRVLAHFLHFHLYEINGVTAALAEELFEKGEQLLGAGEVFAIRPLQLYAITEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIREQLLPFDLSIFKSLHQVEMSHCDAKHVRGLVTSKPTLATMSVRFSAASMKEVLVPEASEFDEWEPEGTTLGGPVTAVIPTWQALTTLDLSHNSISEIDESVKLIPKIEYLDLSHNGVLVVDNLQHLYNLVHLDLSYNKLSSLEGVHTKLGNVKTLNLAGNFLERLSGLHKLYSLVNLDLRDNRIEQLDEVKSIGNLPCLEHVALLNNPLSIIPDYRTKVLSQFGERASEICLDDVATTEKELDTVEVLKAIQKAKDVKSKLSSTEKKVGEDFRLPTAPCIRPSSSPPTAVPTSASLPQPILSNQGIMFVQEEALASSLSSTDSLPPDDRPIAQACSNSMGSLPTGQVAAEDLRDLPGAVGGVSPDHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREEAHGEQGEEEEEEEEEDVAESRYFEMGPPDAEEEEGSGQGEEDEEDEDEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTSLQEFLRQLLTFYKVAGGSQERSQGCFPVYLVYSDKRMVQTAAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTSQHLNVIKADFNPMPSRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLKDLKTVVIAKNPSARPRTQGPLAGGQPAKSRVSAEQRLQETPAEAPAPVPPPAAVPPTASAPAATEAAAPAGAPAAAASAAEAPAVAASAAGAPAAAETPAAAESATEAPAAAESAAETPATAESAAEAPAAAESAAEAPAAAESAAEAPAAAESAAEAPAAAESAAEAPAAAEASAPAGAPAPAGAPAPAGAPAGAQAPAPAGAPAGAPAGAQALAGAQAPAPAGAPAGAQAPVEAQAEVPAQYPSERLIQSTSEENQIPSHLPVCPSLQHIARLRGRAIIDLFHSSIAEVENEELRHLLWSSVVFYQTPGLEVTACVLLSTKAVYFILHDGLRRYFSEPLQDFWHQKNTDYNNSPFHISQCFVLKLSDLQSVNVGLFDQYFRLTGSSPTQVVTCLTRDSYLTHCFLQHLMLVLSSLERTPSPEPIDKDFYSEFGDKNTGKMENYELIHSSRVKFTYPSEEEVGDLTYVVAQKMADPAKNPALSILLYIQAFQVITPQLGRGRGPLRPKTLLLTSAEIFLLDEDYIHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYNPYPQALTLVFDDTQGHDLMGSVTLDHFGEMPGGPGRAGQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

Target ORF information:

RefSeq Version	XM_001058760.5
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nischarin (Nisch), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001058760.5

ORF Insert Sequence:

ATGGCGGCTG CGACACTCAG CTTCGGCCCT GAGCGAGAGG CCGAGCCTGC CAAGGAGGCG 
CGCGTTGTGG GTTCCGAGCT CGTGGACACG TACACGGTAT ATGTCATACA GGTTACCGAT 
GGCAACCATG AGTGGACGAT CAAACACCGC TATAGTGATT TTCATGACCT CCATGAAAAG 
CTTGTTGCTG AGAGAAAAAT TGACAAAACT CTACTTCCAC CCAAAAAGAT AATTGGAAAG 
AACTCGAGAA GCTTGGTAGA AAAGAGGGAG AAAGACCTGG AGGTGTACCT CCAGACTCTC 
CTGAAGACCT TCCCTGATGT GGCTCCCAGA GTTCTGGCCC ACTTCCTGCA TTTTCACCTC 
TATGAAATAA ATGGTGTCAC TGCAGCACTG GCTGAAGAAC TCTTTGAAAA AGGAGAACAG 
CTTCTAGGAG CCGGTGAGGT CTTTGCCATC AGGCCCTTGC AGCTCTATGC TATCACAGAA 
CAGCTGCAGC AGGGAAAGCC TACCTGTGCC AGCGGTGATG CCAAGACTGA CCTTGGACAC 
ATCCTAGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAGA AGGACCTTTT 
GGGACCAGCA ATATTCGAGA GCAGCTCCTG CCCTTTGATC TTTCGATATT CAAGTCTCTT 
CACCAGGTGG AGATGAGTCA CTGTGATGCC AAGCATGTCC GAGGGCTGGT CACCTCCAAG 
CCAACACTGG CCACAATGAG CGTTAGATTC TCAGCAGCCT CAATGAAGGA AGTTCTTGTT 
CCTGAAGCCT CAGAATTTGA TGAGTGGGAG CCTGAAGGCA CAACCCTCGG AGGCCCTGTG 
ACTGCTGTCA TCCCAACATG GCAAGCACTG ACCACTCTAG ACCTGAGCCA CAACAGCATC 
TCTGAGATCG ACGAATCTGT GAAACTGATC CCAAAGATTG AGTATCTGGA CCTGAGTCAC 
AATGGAGTGC TAGTTGTGGA CAACCTGCAG CACCTATACA ACCTCGTGCA CCTTGACCTG 
TCCTACAACA AGCTCTCCTC CCTGGAAGGC GTACACACTA AACTGGGCAA TGTCAAAACC 
CTAAACCTGG CAGGCAACTT CTTAGAGCGT CTGAGCGGTC TGCACAAACT CTATTCCCTG 
GTTAATCTGG ACCTCAGAGA CAACCGGATT GAGCAGTTGG ATGAAGTCAA GAGCATAGGC 
AACCTGCCGT GTCTGGAGCA CGTGGCCCTG CTAAACAACC CTCTGAGCAT CATCCCTGAC 
TACCGGACCA AGGTGCTTTC CCAGTTTGGA GAACGAGCCT CTGAGATCTG TCTAGATGAT 
GTCGCAACCA CAGAGAAGGA ACTGGACACT GTGGAAGTGC TAAAGGCCAT TCAGAAAGCC 
AAAGACGTCA AGTCCAAACT GAGCAGCACA GAAAAGAAGG TTGGTGAGGA CTTCCGGCTC 
CCAACTGCTC CCTGCATCAG ACCCAGCAGC TCCCCTCCCA CTGCCGTTCC CACCTCAGCC 
TCCCTGCCTC AGCCTATCCT CTCCAACCAA GGTATCATGT TCGTACAGGA AGAGGCCCTG 
GCCAGCAGCC TCTCATCCAC GGATAGCCTG CCACCTGATG ACCGGCCCAT TGCCCAAGCC 
TGCTCTAACT CCATGGGATC TCTCCCCACA GGACAGGTGG CCGCTGAAGA CTTAAGGGAC 
TTGCCAGGAG CCGTTGGCGG TGTGAGCCCA GATCATGCAG AACCAGAGGT TCAGGTGGTG 
CCTGGGTCCG GCCAGATCAT CTTCCTGCCC TTCACTTGCA TTGGCTACAC AGCCACCAAC 
CAGGACTTTA TCCAGCGACT CAGCACACTG ATCCGCCAGG CCATTGAACG GCAACTCCCT 
GCCTGGATTG AGGCTGCCAA CCAGCGAGAG GAAGCCCATG GTGAACAGGG GGAGGAAGAG 
GAGGAGGAGG AGGAAGAGGA TGTCGCTGAG AGCCGCTACT TTGAAATGGG ACCCCCAGAC 
GCAGAGGAAG AGGAGGGGAG TGGCCAGGGA GAAGAGGATG AGGAAGACGA GGACGAGGAG 
GCCGAGGAGG AGCGCCTGGC TCTGGAGTGG GCCCTGGGCG CTGATGAGGA CTTCCTGCTG 
GAGCACATCC GCATCCTCAA GGTGCTGTGG TGCTTCCTGA TCCACGTGCA AGGCAGCATC 
CGCCAGTTCG CTGCCTGCCT TGTGCTCACC GACTTCGGCA TCGCTGTCTT TGAGATCCCA 
CACCAAGAGT CCCGAGGCAG CAGCCAGCAC ATCCTCTCAT CCCTGCGCTT CGTCTTCTGC 
TTCCCGCACG GCGACCTCAC CGAGTTTGGC TTCCTCATGC CGGAGCTGTG TCTGGTGCTC 
AAGGTGCGGC ACAGTGAGAA CACGCTCTTC ATCATCTCAG ACGCTGCCAA CTTACACGAG 
TTCCATGCTG ATCTGCGCTC CTGCTTTGCG CCACAGCACA TGGCCATGCT GTGCAGCCCC 
ATCCTCTACG GCAGCCACAC CAGCCTGCAA GAGTTCTTGC GCCAGCTGCT CACCTTCTAC 
AAGGTGGCCG GTGGCTCTCA GGAGCGCAGC CAGGGCTGCT TCCCTGTCTA CCTGGTGTAC 
AGCGACAAGC GCATGGTGCA GACGGCTGCC GGGGACTACT CAGGCAACAT CGAGTGGGCC 
AGCTGCACGC TGTGCTCCGC AGTGCGGCGT TCCTGCTGCG CACCCTCTGA GGCCGTCAAG 
TCTGCCGCCA TCCCCTACTG GCTGCTGCTC ACCTCCCAGC ATCTCAACGT CATCAAGGCC 
GACTTCAACC CCATGCCCAG TCGTGGCACC CACAACTGTC GCAACCGCAA CAGCTTCAAG 
CTTAGCCGCG TCCCGCTCTC CACCGTGCTG CTGGACCCCA CTCGCAGCTG CACCCAGCCA 
CGGGGAGCCT TCGCCGATGG CCATGTGCTC GAGCTGCTTG TTGGCTACCG CTTTGTTACC 
GCCATCTTTG TGCTGCCCCA CGAGAAATTC CACTTCCTGC GAGTCTACAA TCAGCTCCGC 
GCCTCACTGA AGGACCTGAA GACTGTGGTC ATCGCCAAGA ATCCTTCCGC TAGGCCGAGA 
ACCCAAGGCC CCCTCGCGGG TGGCCAGCCT GCCAAAAGCA GGGTCAGTGC TGAGCAGCGG 
CTGCAGGAGA CCCCAGCAGA AGCTCCCGCT CCAGTTCCGC CTCCAGCTGC AGTCCCACCA 
ACAGCTTCAG CCCCAGCCGC AACAGAGGCT GCAGCTCCAG CAGGGGCTCC AGCCGCAGCC 
GCATCTGCAG CAGAGGCTCC AGCTGTAGCC GCATCTGCAG CAGGGGCTCC AGCCGCAGCA 
GAGACTCCAG CCGCAGCCGA ATCTGCAACA GAGGCTCCAG CCGCAGCTGA ATCTGCAGCA 
GAGACTCCAG CCACAGCCGA ATCTGCAGCA GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA 
GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA GAGGCTCCAG CCGCTGCCGA ATCTGCAGCA 
GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA GAGGCTCCAG CAGCGGCAGA GGCTTCAGCT 
CCAGCGGGGG CTCCGGCTCC AGCGGGGGCT CCGGCTCCAG CAGGGGCTCC GGCAGGGGCT 
CAGGCTCCGG CTCCAGCGGG GGCTCCGGCG GGGGCTCCGG CAGGGGCTCA GGCTCTGGCA 
GGGGCTCAGG CTCCGGCTCC AGCGGGGGCT CCGGCAGGGG CTCAGGCTCC AGTGGAGGCC 
CAAGCAGAGG TCCCTGCTCA GTATCCAAGT GAGCGCCTAA TCCAGTCCAC GTCTGAAGAG 
AATCAGATCC CTTCTCACTT GCCAGTCTGC CCATCACTCC AGCACATCGC CCGTCTTCGG 
GGGCGCGCCA TCATTGACCT CTTCCACAGC AGCATTGCCG AGGTTGAAAA CGAGGAGCTA 
AGGCACCTCC TGTGGTCGTC AGTGGTGTTC TACCAGACCC CGGGGCTGGA AGTGACCGCC 
TGTGTGCTGC TGTCCACCAA GGCTGTGTAC TTCATACTGC ATGATGGCCT CCGCCGGTAC 
TTCTCTGAAC CGCTGCAGGA TTTCTGGCAC CAGAAAAACA CTGACTATAA CAACAGTCCT 
TTCCACATCT CTCAGTGCTT TGTGCTCAAA CTCAGTGACC TGCAGTCAGT CAACGTCGGC 
CTTTTCGATC AGTACTTCCG GCTGACGGGC TCTTCCCCGA CGCAGGTGGT CACATGCTTG 
ACTCGAGACA GCTACCTGAC ACACTGCTTC CTGCAGCACC TTATGCTAGT GCTGTCCTCC 
CTGGAGCGCA CACCTTCACC CGAGCCTATT GACAAGGACT TCTACTCAGA ATTTGGGGAC 
AAGAATACAG GGAAAATGGA GAACTATGAG CTGATCCATT CCAGCCGCGT CAAGTTCACC 
TACCCCAGTG AGGAAGAGGT CGGGGACCTG ACCTATGTTG TGGCACAGAA GATGGCTGAC 
CCTGCCAAGA ATCCAGCCCT CAGCATCTTA TTGTACATCC AGGCCTTCCA GGTGATCACA 
CCCCAGCTTG GGCGGGGCAG GGGCCCACTA CGCCCTAAGA CACTCCTACT GACCAGTGCT 
GAGATCTTTC TCCTGGACGA GGACTACATC CACTATCCAT TGCCTGAATT TGCCAAAGAG 
CCACCACAGA GGGACAGATA CCGGCTAGAC GATGGCCGCC GTGTCCGGGA TTTAGACCGG 
GTGCTCATGG GCTACAATCC CTACCCACAG GCCCTCACTC TTGTTTTTGA TGACACGCAG 
GGCCACGACC TCATGGGGAG TGTCACCCTG GACCACTTCG GGGAGATGCC GGGTGGCCCT 
GGTAGGGCTG GGCAGGGCCG GGAGGTGCAG TGGCAGGTGT TTGTGCCCAG TGCTGAGAGC 
CGAGAAAAGC TGATCTCACT GCTCGCACGG CAGTGGGAAG CTCTCTGTGG CAGGGAGCTG 
CCTGTGGAGC TCACTGGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nisch ( XM_001057307.5 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_001057307.5 Rattus norvegicus nischarin (Nisch), transcript variant X1, mRNA.

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CloneID	ORa54530
Clone ID Related Accession (Same CDS sequence)	XM_001058760.5 , XM_001057307.5
Accession Version	XM_001057307.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nischarin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_001057307.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGACACTCAG CTTCGGCCCT GAGCGAGAGG CCGAGCCTGC CAAGGAGGCG 
CGCGTTGTGG GTTCCGAGCT CGTGGACACG TACACGGTAT ATGTCATACA GGTTACCGAT 
GGCAACCATG AGTGGACGAT CAAACACCGC TATAGTGATT TTCATGACCT CCATGAAAAG 
CTTGTTGCTG AGAGAAAAAT TGACAAAACT CTACTTCCAC CCAAAAAGAT AATTGGAAAG 
AACTCGAGAA GCTTGGTAGA AAAGAGGGAG AAAGACCTGG AGGTGTACCT CCAGACTCTC 
CTGAAGACCT TCCCTGATGT GGCTCCCAGA GTTCTGGCCC ACTTCCTGCA TTTTCACCTC 
TATGAAATAA ATGGTGTCAC TGCAGCACTG GCTGAAGAAC TCTTTGAAAA AGGAGAACAG 
CTTCTAGGAG CCGGTGAGGT CTTTGCCATC AGGCCCTTGC AGCTCTATGC TATCACAGAA 
CAGCTGCAGC AGGGAAAGCC TACCTGTGCC AGCGGTGATG CCAAGACTGA CCTTGGACAC 
ATCCTAGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAGA AGGACCTTTT 
GGGACCAGCA ATATTCGAGA GCAGCTCCTG CCCTTTGATC TTTCGATATT CAAGTCTCTT 
CACCAGGTGG AGATGAGTCA CTGTGATGCC AAGCATGTCC GAGGGCTGGT CACCTCCAAG 
CCAACACTGG CCACAATGAG CGTTAGATTC TCAGCAGCCT CAATGAAGGA AGTTCTTGTT 
CCTGAAGCCT CAGAATTTGA TGAGTGGGAG CCTGAAGGCA CAACCCTCGG AGGCCCTGTG 
ACTGCTGTCA TCCCAACATG GCAAGCACTG ACCACTCTAG ACCTGAGCCA CAACAGCATC 
TCTGAGATCG ACGAATCTGT GAAACTGATC CCAAAGATTG AGTATCTGGA CCTGAGTCAC 
AATGGAGTGC TAGTTGTGGA CAACCTGCAG CACCTATACA ACCTCGTGCA CCTTGACCTG 
TCCTACAACA AGCTCTCCTC CCTGGAAGGC GTACACACTA AACTGGGCAA TGTCAAAACC 
CTAAACCTGG CAGGCAACTT CTTAGAGCGT CTGAGCGGTC TGCACAAACT CTATTCCCTG 
GTTAATCTGG ACCTCAGAGA CAACCGGATT GAGCAGTTGG ATGAAGTCAA GAGCATAGGC 
AACCTGCCGT GTCTGGAGCA CGTGGCCCTG CTAAACAACC CTCTGAGCAT CATCCCTGAC 
TACCGGACCA AGGTGCTTTC CCAGTTTGGA GAACGAGCCT CTGAGATCTG TCTAGATGAT 
GTCGCAACCA CAGAGAAGGA ACTGGACACT GTGGAAGTGC TAAAGGCCAT TCAGAAAGCC 
AAAGACGTCA AGTCCAAACT GAGCAGCACA GAAAAGAAGG TTGGTGAGGA CTTCCGGCTC 
CCAACTGCTC CCTGCATCAG ACCCAGCAGC TCCCCTCCCA CTGCCGTTCC CACCTCAGCC 
TCCCTGCCTC AGCCTATCCT CTCCAACCAA GGTATCATGT TCGTACAGGA AGAGGCCCTG 
GCCAGCAGCC TCTCATCCAC GGATAGCCTG CCACCTGATG ACCGGCCCAT TGCCCAAGCC 
TGCTCTAACT CCATGGGATC TCTCCCCACA GGACAGGTGG CCGCTGAAGA CTTAAGGGAC 
TTGCCAGGAG CCGTTGGCGG TGTGAGCCCA GATCATGCAG AACCAGAGGT TCAGGTGGTG 
CCTGGGTCCG GCCAGATCAT CTTCCTGCCC TTCACTTGCA TTGGCTACAC AGCCACCAAC 
CAGGACTTTA TCCAGCGACT CAGCACACTG ATCCGCCAGG CCATTGAACG GCAACTCCCT 
GCCTGGATTG AGGCTGCCAA CCAGCGAGAG GAAGCCCATG GTGAACAGGG GGAGGAAGAG 
GAGGAGGAGG AGGAAGAGGA TGTCGCTGAG AGCCGCTACT TTGAAATGGG ACCCCCAGAC 
GCAGAGGAAG AGGAGGGGAG TGGCCAGGGA GAAGAGGATG AGGAAGACGA GGACGAGGAG 
GCCGAGGAGG AGCGCCTGGC TCTGGAGTGG GCCCTGGGCG CTGATGAGGA CTTCCTGCTG 
GAGCACATCC GCATCCTCAA GGTGCTGTGG TGCTTCCTGA TCCACGTGCA AGGCAGCATC 
CGCCAGTTCG CTGCCTGCCT TGTGCTCACC GACTTCGGCA TCGCTGTCTT TGAGATCCCA 
CACCAAGAGT CCCGAGGCAG CAGCCAGCAC ATCCTCTCAT CCCTGCGCTT CGTCTTCTGC 
TTCCCGCACG GCGACCTCAC CGAGTTTGGC TTCCTCATGC CGGAGCTGTG TCTGGTGCTC 
AAGGTGCGGC ACAGTGAGAA CACGCTCTTC ATCATCTCAG ACGCTGCCAA CTTACACGAG 
TTCCATGCTG ATCTGCGCTC CTGCTTTGCG CCACAGCACA TGGCCATGCT GTGCAGCCCC 
ATCCTCTACG GCAGCCACAC CAGCCTGCAA GAGTTCTTGC GCCAGCTGCT CACCTTCTAC 
AAGGTGGCCG GTGGCTCTCA GGAGCGCAGC CAGGGCTGCT TCCCTGTCTA CCTGGTGTAC 
AGCGACAAGC GCATGGTGCA GACGGCTGCC GGGGACTACT CAGGCAACAT CGAGTGGGCC 
AGCTGCACGC TGTGCTCCGC AGTGCGGCGT TCCTGCTGCG CACCCTCTGA GGCCGTCAAG 
TCTGCCGCCA TCCCCTACTG GCTGCTGCTC ACCTCCCAGC ATCTCAACGT CATCAAGGCC 
GACTTCAACC CCATGCCCAG TCGTGGCACC CACAACTGTC GCAACCGCAA CAGCTTCAAG 
CTTAGCCGCG TCCCGCTCTC CACCGTGCTG CTGGACCCCA CTCGCAGCTG CACCCAGCCA 
CGGGGAGCCT TCGCCGATGG CCATGTGCTC GAGCTGCTTG TTGGCTACCG CTTTGTTACC 
GCCATCTTTG TGCTGCCCCA CGAGAAATTC CACTTCCTGC GAGTCTACAA TCAGCTCCGC 
GCCTCACTGA AGGACCTGAA GACTGTGGTC ATCGCCAAGA ATCCTTCCGC TAGGCCGAGA 
ACCCAAGGCC CCCTCGCGGG TGGCCAGCCT GCCAAAAGCA GGGTCAGTGC TGAGCAGCGG 
CTGCAGGAGA CCCCAGCAGA AGCTCCCGCT CCAGTTCCGC CTCCAGCTGC AGTCCCACCA 
ACAGCTTCAG CCCCAGCCGC AACAGAGGCT GCAGCTCCAG CAGGGGCTCC AGCCGCAGCC 
GCATCTGCAG CAGAGGCTCC AGCTGTAGCC GCATCTGCAG CAGGGGCTCC AGCCGCAGCA 
GAGACTCCAG CCGCAGCCGA ATCTGCAACA GAGGCTCCAG CCGCAGCTGA ATCTGCAGCA 
GAGACTCCAG CCACAGCCGA ATCTGCAGCA GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA 
GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA GAGGCTCCAG CCGCTGCCGA ATCTGCAGCA 
GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA GAGGCTCCAG CAGCGGCAGA GGCTTCAGCT 
CCAGCGGGGG CTCCGGCTCC AGCGGGGGCT CCGGCTCCAG CAGGGGCTCC GGCAGGGGCT 
CAGGCTCCGG CTCCAGCGGG GGCTCCGGCG GGGGCTCCGG CAGGGGCTCA GGCTCTGGCA 
GGGGCTCAGG CTCCGGCTCC AGCGGGGGCT CCGGCAGGGG CTCAGGCTCC AGTGGAGGCC 
CAAGCAGAGG TCCCTGCTCA GTATCCAAGT GAGCGCCTAA TCCAGTCCAC GTCTGAAGAG 
AATCAGATCC CTTCTCACTT GCCAGTCTGC CCATCACTCC AGCACATCGC CCGTCTTCGG 
GGGCGCGCCA TCATTGACCT CTTCCACAGC AGCATTGCCG AGGTTGAAAA CGAGGAGCTA 
AGGCACCTCC TGTGGTCGTC AGTGGTGTTC TACCAGACCC CGGGGCTGGA AGTGACCGCC 
TGTGTGCTGC TGTCCACCAA GGCTGTGTAC TTCATACTGC ATGATGGCCT CCGCCGGTAC 
TTCTCTGAAC CGCTGCAGGA TTTCTGGCAC CAGAAAAACA CTGACTATAA CAACAGTCCT 
TTCCACATCT CTCAGTGCTT TGTGCTCAAA CTCAGTGACC TGCAGTCAGT CAACGTCGGC 
CTTTTCGATC AGTACTTCCG GCTGACGGGC TCTTCCCCGA CGCAGGTGGT CACATGCTTG 
ACTCGAGACA GCTACCTGAC ACACTGCTTC CTGCAGCACC TTATGCTAGT GCTGTCCTCC 
CTGGAGCGCA CACCTTCACC CGAGCCTATT GACAAGGACT TCTACTCAGA ATTTGGGGAC 
AAGAATACAG GGAAAATGGA GAACTATGAG CTGATCCATT CCAGCCGCGT CAAGTTCACC 
TACCCCAGTG AGGAAGAGGT CGGGGACCTG ACCTATGTTG TGGCACAGAA GATGGCTGAC 
CCTGCCAAGA ATCCAGCCCT CAGCATCTTA TTGTACATCC AGGCCTTCCA GGTGATCACA 
CCCCAGCTTG GGCGGGGCAG GGGCCCACTA CGCCCTAAGA CACTCCTACT GACCAGTGCT 
GAGATCTTTC TCCTGGACGA GGACTACATC CACTATCCAT TGCCTGAATT TGCCAAAGAG 
CCACCACAGA GGGACAGATA CCGGCTAGAC GATGGCCGCC GTGTCCGGGA TTTAGACCGG 
GTGCTCATGG GCTACAATCC CTACCCACAG GCCCTCACTC TTGTTTTTGA TGACACGCAG 
GGCCACGACC TCATGGGGAG TGTCACCCTG GACCACTTCG GGGAGATGCC GGGTGGCCCT 
GGTAGGGCTG GGCAGGGCCG GGAGGTGCAG TGGCAGGTGT TTGTGCCCAG TGCTGAGAGC 
CGAGAAAAGC TGATCTCACT GCTCGCACGG CAGTGGGAAG CTCTCTGTGG CAGGGAGCTG 
CCTGTGGAGC TCACTGGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001057307.5
CDS	110..5050
Translation	MAAATLSFGPEREAEPAKEARVVGSELVDTYTVYVIQVTDGNHEWTIKHRYSDFHDLHEKLVAERKIDKTLLPPKKIIGKNSRSLVEKREKDLEVYLQTLLKTFPDVAPRVLAHFLHFHLYEINGVTAALAEELFEKGEQLLGAGEVFAIRPLQLYAITEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIREQLLPFDLSIFKSLHQVEMSHCDAKHVRGLVTSKPTLATMSVRFSAASMKEVLVPEASEFDEWEPEGTTLGGPVTAVIPTWQALTTLDLSHNSISEIDESVKLIPKIEYLDLSHNGVLVVDNLQHLYNLVHLDLSYNKLSSLEGVHTKLGNVKTLNLAGNFLERLSGLHKLYSLVNLDLRDNRIEQLDEVKSIGNLPCLEHVALLNNPLSIIPDYRTKVLSQFGERASEICLDDVATTEKELDTVEVLKAIQKAKDVKSKLSSTEKKVGEDFRLPTAPCIRPSSSPPTAVPTSASLPQPILSNQGIMFVQEEALASSLSSTDSLPPDDRPIAQACSNSMGSLPTGQVAAEDLRDLPGAVGGVSPDHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREEAHGEQGEEEEEEEEEDVAESRYFEMGPPDAEEEEGSGQGEEDEEDEDEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTSLQEFLRQLLTFYKVAGGSQERSQGCFPVYLVYSDKRMVQTAAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTSQHLNVIKADFNPMPSRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLKDLKTVVIAKNPSARPRTQGPLAGGQPAKSRVSAEQRLQETPAEAPAPVPPPAAVPPTASAPAATEAAAPAGAPAAAASAAEAPAVAASAAGAPAAAETPAAAESATEAPAAAESAAETPATAESAAEAPAAAESAAEAPAAAESAAEAPAAAESAAEAPAAAESAAEAPAAAEASAPAGAPAPAGAPAPAGAPAGAQAPAPAGAPAGAPAGAQALAGAQAPAPAGAPAGAQAPVEAQAEVPAQYPSERLIQSTSEENQIPSHLPVCPSLQHIARLRGRAIIDLFHSSIAEVENEELRHLLWSSVVFYQTPGLEVTACVLLSTKAVYFILHDGLRRYFSEPLQDFWHQKNTDYNNSPFHISQCFVLKLSDLQSVNVGLFDQYFRLTGSSPTQVVTCLTRDSYLTHCFLQHLMLVLSSLERTPSPEPIDKDFYSEFGDKNTGKMENYELIHSSRVKFTYPSEEEVGDLTYVVAQKMADPAKNPALSILLYIQAFQVITPQLGRGRGPLRPKTLLLTSAEIFLLDEDYIHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYNPYPQALTLVFDDTQGHDLMGSVTLDHFGEMPGGPGRAGQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

Target ORF information:

RefSeq Version	XM_001057307.5
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nischarin (Nisch), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001057307.5

ORF Insert Sequence:

ATGGCGGCTG CGACACTCAG CTTCGGCCCT GAGCGAGAGG CCGAGCCTGC CAAGGAGGCG 
CGCGTTGTGG GTTCCGAGCT CGTGGACACG TACACGGTAT ATGTCATACA GGTTACCGAT 
GGCAACCATG AGTGGACGAT CAAACACCGC TATAGTGATT TTCATGACCT CCATGAAAAG 
CTTGTTGCTG AGAGAAAAAT TGACAAAACT CTACTTCCAC CCAAAAAGAT AATTGGAAAG 
AACTCGAGAA GCTTGGTAGA AAAGAGGGAG AAAGACCTGG AGGTGTACCT CCAGACTCTC 
CTGAAGACCT TCCCTGATGT GGCTCCCAGA GTTCTGGCCC ACTTCCTGCA TTTTCACCTC 
TATGAAATAA ATGGTGTCAC TGCAGCACTG GCTGAAGAAC TCTTTGAAAA AGGAGAACAG 
CTTCTAGGAG CCGGTGAGGT CTTTGCCATC AGGCCCTTGC AGCTCTATGC TATCACAGAA 
CAGCTGCAGC AGGGAAAGCC TACCTGTGCC AGCGGTGATG CCAAGACTGA CCTTGGACAC 
ATCCTAGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAGA AGGACCTTTT 
GGGACCAGCA ATATTCGAGA GCAGCTCCTG CCCTTTGATC TTTCGATATT CAAGTCTCTT 
CACCAGGTGG AGATGAGTCA CTGTGATGCC AAGCATGTCC GAGGGCTGGT CACCTCCAAG 
CCAACACTGG CCACAATGAG CGTTAGATTC TCAGCAGCCT CAATGAAGGA AGTTCTTGTT 
CCTGAAGCCT CAGAATTTGA TGAGTGGGAG CCTGAAGGCA CAACCCTCGG AGGCCCTGTG 
ACTGCTGTCA TCCCAACATG GCAAGCACTG ACCACTCTAG ACCTGAGCCA CAACAGCATC 
TCTGAGATCG ACGAATCTGT GAAACTGATC CCAAAGATTG AGTATCTGGA CCTGAGTCAC 
AATGGAGTGC TAGTTGTGGA CAACCTGCAG CACCTATACA ACCTCGTGCA CCTTGACCTG 
TCCTACAACA AGCTCTCCTC CCTGGAAGGC GTACACACTA AACTGGGCAA TGTCAAAACC 
CTAAACCTGG CAGGCAACTT CTTAGAGCGT CTGAGCGGTC TGCACAAACT CTATTCCCTG 
GTTAATCTGG ACCTCAGAGA CAACCGGATT GAGCAGTTGG ATGAAGTCAA GAGCATAGGC 
AACCTGCCGT GTCTGGAGCA CGTGGCCCTG CTAAACAACC CTCTGAGCAT CATCCCTGAC 
TACCGGACCA AGGTGCTTTC CCAGTTTGGA GAACGAGCCT CTGAGATCTG TCTAGATGAT 
GTCGCAACCA CAGAGAAGGA ACTGGACACT GTGGAAGTGC TAAAGGCCAT TCAGAAAGCC 
AAAGACGTCA AGTCCAAACT GAGCAGCACA GAAAAGAAGG TTGGTGAGGA CTTCCGGCTC 
CCAACTGCTC CCTGCATCAG ACCCAGCAGC TCCCCTCCCA CTGCCGTTCC CACCTCAGCC 
TCCCTGCCTC AGCCTATCCT CTCCAACCAA GGTATCATGT TCGTACAGGA AGAGGCCCTG 
GCCAGCAGCC TCTCATCCAC GGATAGCCTG CCACCTGATG ACCGGCCCAT TGCCCAAGCC 
TGCTCTAACT CCATGGGATC TCTCCCCACA GGACAGGTGG CCGCTGAAGA CTTAAGGGAC 
TTGCCAGGAG CCGTTGGCGG TGTGAGCCCA GATCATGCAG AACCAGAGGT TCAGGTGGTG 
CCTGGGTCCG GCCAGATCAT CTTCCTGCCC TTCACTTGCA TTGGCTACAC AGCCACCAAC 
CAGGACTTTA TCCAGCGACT CAGCACACTG ATCCGCCAGG CCATTGAACG GCAACTCCCT 
GCCTGGATTG AGGCTGCCAA CCAGCGAGAG GAAGCCCATG GTGAACAGGG GGAGGAAGAG 
GAGGAGGAGG AGGAAGAGGA TGTCGCTGAG AGCCGCTACT TTGAAATGGG ACCCCCAGAC 
GCAGAGGAAG AGGAGGGGAG TGGCCAGGGA GAAGAGGATG AGGAAGACGA GGACGAGGAG 
GCCGAGGAGG AGCGCCTGGC TCTGGAGTGG GCCCTGGGCG CTGATGAGGA CTTCCTGCTG 
GAGCACATCC GCATCCTCAA GGTGCTGTGG TGCTTCCTGA TCCACGTGCA AGGCAGCATC 
CGCCAGTTCG CTGCCTGCCT TGTGCTCACC GACTTCGGCA TCGCTGTCTT TGAGATCCCA 
CACCAAGAGT CCCGAGGCAG CAGCCAGCAC ATCCTCTCAT CCCTGCGCTT CGTCTTCTGC 
TTCCCGCACG GCGACCTCAC CGAGTTTGGC TTCCTCATGC CGGAGCTGTG TCTGGTGCTC 
AAGGTGCGGC ACAGTGAGAA CACGCTCTTC ATCATCTCAG ACGCTGCCAA CTTACACGAG 
TTCCATGCTG ATCTGCGCTC CTGCTTTGCG CCACAGCACA TGGCCATGCT GTGCAGCCCC 
ATCCTCTACG GCAGCCACAC CAGCCTGCAA GAGTTCTTGC GCCAGCTGCT CACCTTCTAC 
AAGGTGGCCG GTGGCTCTCA GGAGCGCAGC CAGGGCTGCT TCCCTGTCTA CCTGGTGTAC 
AGCGACAAGC GCATGGTGCA GACGGCTGCC GGGGACTACT CAGGCAACAT CGAGTGGGCC 
AGCTGCACGC TGTGCTCCGC AGTGCGGCGT TCCTGCTGCG CACCCTCTGA GGCCGTCAAG 
TCTGCCGCCA TCCCCTACTG GCTGCTGCTC ACCTCCCAGC ATCTCAACGT CATCAAGGCC 
GACTTCAACC CCATGCCCAG TCGTGGCACC CACAACTGTC GCAACCGCAA CAGCTTCAAG 
CTTAGCCGCG TCCCGCTCTC CACCGTGCTG CTGGACCCCA CTCGCAGCTG CACCCAGCCA 
CGGGGAGCCT TCGCCGATGG CCATGTGCTC GAGCTGCTTG TTGGCTACCG CTTTGTTACC 
GCCATCTTTG TGCTGCCCCA CGAGAAATTC CACTTCCTGC GAGTCTACAA TCAGCTCCGC 
GCCTCACTGA AGGACCTGAA GACTGTGGTC ATCGCCAAGA ATCCTTCCGC TAGGCCGAGA 
ACCCAAGGCC CCCTCGCGGG TGGCCAGCCT GCCAAAAGCA GGGTCAGTGC TGAGCAGCGG 
CTGCAGGAGA CCCCAGCAGA AGCTCCCGCT CCAGTTCCGC CTCCAGCTGC AGTCCCACCA 
ACAGCTTCAG CCCCAGCCGC AACAGAGGCT GCAGCTCCAG CAGGGGCTCC AGCCGCAGCC 
GCATCTGCAG CAGAGGCTCC AGCTGTAGCC GCATCTGCAG CAGGGGCTCC AGCCGCAGCA 
GAGACTCCAG CCGCAGCCGA ATCTGCAACA GAGGCTCCAG CCGCAGCTGA ATCTGCAGCA 
GAGACTCCAG CCACAGCCGA ATCTGCAGCA GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA 
GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA GAGGCTCCAG CCGCTGCCGA ATCTGCAGCA 
GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA GAGGCTCCAG CAGCGGCAGA GGCTTCAGCT 
CCAGCGGGGG CTCCGGCTCC AGCGGGGGCT CCGGCTCCAG CAGGGGCTCC GGCAGGGGCT 
CAGGCTCCGG CTCCAGCGGG GGCTCCGGCG GGGGCTCCGG CAGGGGCTCA GGCTCTGGCA 
GGGGCTCAGG CTCCGGCTCC AGCGGGGGCT CCGGCAGGGG CTCAGGCTCC AGTGGAGGCC 
CAAGCAGAGG TCCCTGCTCA GTATCCAAGT GAGCGCCTAA TCCAGTCCAC GTCTGAAGAG 
AATCAGATCC CTTCTCACTT GCCAGTCTGC CCATCACTCC AGCACATCGC CCGTCTTCGG 
GGGCGCGCCA TCATTGACCT CTTCCACAGC AGCATTGCCG AGGTTGAAAA CGAGGAGCTA 
AGGCACCTCC TGTGGTCGTC AGTGGTGTTC TACCAGACCC CGGGGCTGGA AGTGACCGCC 
TGTGTGCTGC TGTCCACCAA GGCTGTGTAC TTCATACTGC ATGATGGCCT CCGCCGGTAC 
TTCTCTGAAC CGCTGCAGGA TTTCTGGCAC CAGAAAAACA CTGACTATAA CAACAGTCCT 
TTCCACATCT CTCAGTGCTT TGTGCTCAAA CTCAGTGACC TGCAGTCAGT CAACGTCGGC 
CTTTTCGATC AGTACTTCCG GCTGACGGGC TCTTCCCCGA CGCAGGTGGT CACATGCTTG 
ACTCGAGACA GCTACCTGAC ACACTGCTTC CTGCAGCACC TTATGCTAGT GCTGTCCTCC 
CTGGAGCGCA CACCTTCACC CGAGCCTATT GACAAGGACT TCTACTCAGA ATTTGGGGAC 
AAGAATACAG GGAAAATGGA GAACTATGAG CTGATCCATT CCAGCCGCGT CAAGTTCACC 
TACCCCAGTG AGGAAGAGGT CGGGGACCTG ACCTATGTTG TGGCACAGAA GATGGCTGAC 
CCTGCCAAGA ATCCAGCCCT CAGCATCTTA TTGTACATCC AGGCCTTCCA GGTGATCACA 
CCCCAGCTTG GGCGGGGCAG GGGCCCACTA CGCCCTAAGA CACTCCTACT GACCAGTGCT 
GAGATCTTTC TCCTGGACGA GGACTACATC CACTATCCAT TGCCTGAATT TGCCAAAGAG 
CCACCACAGA GGGACAGATA CCGGCTAGAC GATGGCCGCC GTGTCCGGGA TTTAGACCGG 
GTGCTCATGG GCTACAATCC CTACCCACAG GCCCTCACTC TTGTTTTTGA TGACACGCAG 
GGCCACGACC TCATGGGGAG TGTCACCCTG GACCACTTCG GGGAGATGCC GGGTGGCCCT 
GGTAGGGCTG GGCAGGGCCG GGAGGTGCAG TGGCAGGTGT TTGTGCCCAG TGCTGAGAGC 
CGAGAAAAGC TGATCTCACT GCTCGCACGG CAGTGGGAAG CTCTCTGTGG CAGGGAGCTG 
CCTGTGGAGC TCACTGGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nisch ( XM_017600295.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455784.1 Rattus norvegicus nischarin (Nisch), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa54531
Clone ID Related Accession (Same CDS sequence)	XM_017600295.1 , XM_017587539.1
Accession Version	XM_017600295.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nischarin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGACACTCAG CTTCGGCCCT GAGCGAGAGG CCGAGCCTGC CAAGGAGGCG 
CGCGTTGTGG GTTCCGAGCT CGTGGACACG TACACGGTAT ATGTCATACA GGTTACCGAT 
GGCAACCATG AGTGGACGAT CAAACACCGC TATAGTGATT TTCATGACCT CCATGAAAAG 
CTTGTTGCTG AGAGAAAAAT TGACAAAACT CTACTTCCAC CCAAAAAGAT AATTGGAAAG 
AACTCGAGAA GCTTGGTAGA AAAGAGGGAG AAAGACCTGG AGGTGTACCT CCAGACTCTC 
CTGAAGACCT TCCCTGATGT GGCTCCCAGA GTTCTGGCCC ACTTCCTGCA TTTTCACCTC 
TATGAAATAA ATGGTGTCAC TGCAGCACTG GCTGAAGAAC TCTTTGAAAA AGGAGAACAG 
CTTCTAGGAG CCGGTGAGGT CTTTGCCATC AGGCCCTTGC AGCTCTATGC TATCACAGAA 
CAGCTGCAGC AGGGAAAGCC TACCTGTGCC AGCGGTGATG CCAAGACTGA CCTTGGACAC 
ATCCTAGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAGA AGGACCTTTT 
GGGACCAGCA ATATTCGAGA GCAGCTCCTG CCCTTTGATC TTTCGATATT CAAGTCTCTT 
CACCAGGTGG AGATGAGTCA CTGTGATGCC AAGCATGTCC GAGGGCTGGT CACCTCCAAG 
CCAACACTGG CCACAATGAG CGTTAGATTC TCAGCAGCCT CAATGAAGGA AGTTCTTGTT 
CCTGAAGCCT CAGAATTTGA TGAGTGGGAG CCTGAAGGCA CAACCCTCGG AGGCCCTGTG 
ACTGCTGTCA TCCCAACATG GCAAGCACTG ACCACTCTAG ACCTGAGCCA CAACAGCATC 
TCTGAGATCG ACGAATCTGT GAAACTGATC CCAAAGATTG AGTATCTGGA CCTGAGTCAC 
AATGGAGTGC TAGTTGTGGA CAACCTGCAG CACCTATACA ACCTCGTGCA CCTTGACCTG 
TCCTACAACA AGCTCTCCTC CCTGGAAGGC GTACACACTA AACTGGGCAA TGTCAAAACC 
CTAAACCTGG CAGGCAACTT CTTAGAGCGT CTGAGCGGTC TGCACAAACT CTATTCCCTG 
GTTAATCTGG ACCTCAGAGA CAACCGGATT GAGCAGTTGG ATGAAGTCAA GAGCATAGGC 
AACCTGCCGT GTCTGGAGCA CGTGGCCCTG CTAAACAACC CTCTGAGCAT CATCCCTGAC 
TACCGGACCA AGGTGCTTTC CCAGTTTGGA GAACGAGCCT CTGAGATCTG TCTAGATGAT 
GTCGCAACCA CAGAGAAGGA ACTGGACACT GTGGAAGTGC TAAAGGCCAT TCAGAAAGCC 
AAAGACGTCA AGTCCAAACT GAGCAGCACA GAAAAGAAGG TTGGTGAGGA CTTCCGGCTC 
CCAACTGCTC CCTGCATCAG ACCCAGCAGC TCCCCTCCCA CTGCCGTTCC CACCTCAGCC 
TCCCTGCCTC AGCCTATCCT CTCCAACCAA GGTATCATGT TCGTACAGGA AGAGGCCCTG 
GCCAGCAGCC TCTCATCCAC GGATAGCCTG CCACCTGATG ACCGGCCCAT TGCCCAAGCC 
TGCTCTAACT CCATGGGATC TCTCCCCACA GGACAGGTGG CCGCTGAAGA CTTAAGGGAC 
TTGCCAGGAG CCGTTGGCGG TGTGAGCCCA GATCATGCAG AACCAGAGGT TCAGGTGGTG 
CCTGGGTCCG GCCAGATCAT CTTCCTGCCC TTCACTTGCA TTGGCTACAC AGCCACCAAC 
CAGGACTTTA TCCAGCGACT CAGCACACTG ATCCGCCAGG CCATTGAACG GCAACTCCCT 
GCCTGGATTG AGGCTGCCAA CCAGCGAGAG GAAGCCCATG GTGAACAGGG GGAGGAAGAG 
GAGGAGGAGG AGGAAGAGGA TGTCGCTGAG AGCCGCTACT TTGAAATGGG ACCCCCAGAC 
GCAGAGGAAG AGGAGGGGAG TGGCCAGGGA GAAGAGGATG AGGAAGACGA GGACGAGGAG 
GCCGAGGAGG AGCGCCTGGC TCTGGAGTGG GCCCTGGGCG CTGATGAGGA CTTCCTGCTG 
GAGCACATCC GCATCCTCAA GGTGCTGTGG TGCTTCCTGA TCCACGTGCA AGGCAGCATC 
CGCCAGTTCG CTGCCTGCCT TGTGCTCACC GACTTCGGCA TCGCTGTCTT TGAGATCCCA 
CACCAAGAGT CCCGAGGCAG CAGCCAGCAC ATCCTCTCAT CCCTGCGCTT CGTCTTCTGC 
TTCCCGCACG GCGACCTCAC CGAGTTTGGC TTCCTCATGC CGGAGCTGTG TCTGGTGCTC 
AAGGTGCGGC ACAGTGAGAA CACGCTCTTC ATCATCTCAG ACGCTGCCAA CTTACACGAG 
TTCCATGCTG ATCTGCGCTC CTGCTTTGCG CCACAGCACA TGGCCATGCT GTGCAGCCCC 
ATCCTCTACG GCAGCCACAC CAGCCTGCAA GAGTTCTTGC GCCAGCTGCT CACCTTCTAC 
AAGGTGGCCG GTGGCTCTCA GGAGCGCAGC CAGGGCTGCT TCCCTGTCTA CCTGGTGTAC 
AGCGACAAGC GCATGGTGCA GACGGCTGCC GGGGACTACT CAGGCAACAT CGAGTGGGCC 
AGCTGCACGC TGTGCTCCGC AGTGCGGCGT TCCTGCTGCG CACCCTCTGA GGCCGTCAAG 
TCTGCCGCCA TCCCCTACTG GCTGCTGCTC ACCTCCCAGC ATCTCAACGT CATCAAGGCC 
GACTTCAACC CCATGCCCAG TCGTGGCACC CACAACTGTC GCAACCGCAA CAGCTTCAAG 
CTTAGCCGCG TCCCGCTCTC CACCGTGCTG CTGGACCCCA CTCGCAGCTG CACCCAGCCA 
CGGGGAGCCT TCGCCGATGG CCATGTGCTC GAGCTGCTTG TTGGCTACCG CTTTGTTACC 
GCCATCTTTG TGCTGCCCCA CGAGAAATTC CACTTCCTGC GAGTCTACAA TCAGCTCCGC 
GCCTCACTGA AGGACCTGAA GACTGTGGTC ATCGCCAAGA ATCCTTCCGC TAGGCCGAGA 
ACCCAAGGCC CCCTCGCGGG TGGCCAGCCT GCCAAAAGCA GGGTCAGTGC TGAGCAGCGG 
CTGCAGGAGA CCCCAGCAGA AGCTCCCGCT CCAGTTCCGC CTCCAGCTGC AGTCCCACCA 
ACAGCTTCAG CCCCAGCCGC AACAGAGGCT GCAGCTCCAG CAGGGGCTCC AGCCGCAGCC 
GCATCTGCAG CAGAGGCTCC AGCTGTAGCC GCATCTGCAG CAGGGGCTCC AGCCGCAGCA 
GAGACTCCAG CCGCAGCCGA ATCTGCAACA GAGGCTCCAG CCGCAGCTGA ATCTGCAGCA 
GAGACTCCAG CCACAGCCGA ATCTGCAGCA GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA 
GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA GAGGCTCCAG CCGCTGCCGA ATCTGCAGCA 
GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA GAGGCTCCAG CAGCGGCAGA GGCTTCAGCT 
CCAGCGGGGG CTCCGGCTCC AGCGGGGGCT CTGGCAGGGG CTCAGGCTCC GGCTCCAGCG 
GGGGCTCCGG CAGGGGCTCA GGCTCCAGTG GAGGCCCAAG CAGAGGTCCC TGCTCAGTAT 
CCAAGTGAGC GCCTAATCCA GTCCACGTCT GAAGAGAATC AGATCCCTTC TCACTTGCCA 
GTCTGCCCAT CACTCCAGCA CATCGCCCGT CTTCGGGGGC GCGCCATCAT TGACCTCTTC 
CACAGCAGCA TTGCCGAGGT TGAAAACGAG GAGCTAAGGC ACCTCCTGTG GTCGTCAGTG 
GTGTTCTACC AGACCCCGGG GCTGGAAGTG ACCGCCTGTG TGCTGCTGTC CACCAAGGCT 
GTGTACTTCA TACTGCATGA TGGCCTCCGC CGGTACTTCT CTGAACCGCT GCAGGATTTC 
TGGCACCAGA AAAACACTGA CTATAACAAC AGTCCTTTCC ACATCTCTCA GTGCTTTGTG 
CTCAAACTCA GTGACCTGCA GTCAGTCAAC GTCGGCCTTT TCGATCAGTA CTTCCGGCTG 
ACGGGCTCTT CCCCGACGCA GGTGGTCACA TGCTTGACTC GAGACAGCTA CCTGACACAC 
TGCTTCCTGC AGCACCTTAT GCTAGTGCTG TCCTCCCTGG AGCGCACACC TTCACCCGAG 
CCTATTGACA AGGACTTCTA CTCAGAATTT GGGGACAAGA ATACAGGGAA AATGGAGAAC 
TATGAGCTGA TCCATTCCAG CCGCGTCAAG TTCACCTACC CCAGTGAGGA AGAGGTCGGG 
GACCTGACCT ATGTTGTGGC ACAGAAGATG GCTGACCCTG CCAAGAATCC AGCCCTCAGC 
ATCTTATTGT ACATCCAGGC CTTCCAGGTG ATCACACCCC AGCTTGGGCG GGGCAGGGGC 
CCACTACGCC CTAAGACACT CCTACTGACC AGTGCTGAGA TCTTTCTCCT GGACGAGGAC 
TACATCCACT ATCCATTGCC TGAATTTGCC AAAGAGCCAC CACAGAGGGA CAGATACCGG 
CTAGACGATG GCCGCCGTGT CCGGGATTTA GACCGGGTGC TCATGGGCTA CAATCCCTAC 
CCACAGGCCC TCACTCTTGT TTTTGATGAC ACGCAGGGCC ACGACCTCAT GGGGAGTGTC 
ACCCTGGACC ACTTCGGGGA GATGCCGGGT GGCCCTGGTA GGGCTGGGCA GGGCCGGGAG 
GTGCAGTGGC AGGTGTTTGT GCCCAGTGCT GAGAGCCGAG AAAAGCTGAT CTCACTGCTC 
GCACGGCAGT GGGAAGCTCT CTGTGGCAGG GAGCTGCCTG TGGAGCTCAC TGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455784.1
CDS	109..4965
Translation	MAAATLSFGPEREAEPAKEARVVGSELVDTYTVYVIQVTDGNHEWTIKHRYSDFHDLHEKLVAERKIDKTLLPPKKIIGKNSRSLVEKREKDLEVYLQTLLKTFPDVAPRVLAHFLHFHLYEINGVTAALAEELFEKGEQLLGAGEVFAIRPLQLYAITEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIREQLLPFDLSIFKSLHQVEMSHCDAKHVRGLVTSKPTLATMSVRFSAASMKEVLVPEASEFDEWEPEGTTLGGPVTAVIPTWQALTTLDLSHNSISEIDESVKLIPKIEYLDLSHNGVLVVDNLQHLYNLVHLDLSYNKLSSLEGVHTKLGNVKTLNLAGNFLERLSGLHKLYSLVNLDLRDNRIEQLDEVKSIGNLPCLEHVALLNNPLSIIPDYRTKVLSQFGERASEICLDDVATTEKELDTVEVLKAIQKAKDVKSKLSSTEKKVGEDFRLPTAPCIRPSSSPPTAVPTSASLPQPILSNQGIMFVQEEALASSLSSTDSLPPDDRPIAQACSNSMGSLPTGQVAAEDLRDLPGAVGGVSPDHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREEAHGEQGEEEEEEEEEDVAESRYFEMGPPDAEEEEGSGQGEEDEEDEDEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTSLQEFLRQLLTFYKVAGGSQERSQGCFPVYLVYSDKRMVQTAAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTSQHLNVIKADFNPMPSRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLKDLKTVVIAKNPSARPRTQGPLAGGQPAKSRVSAEQRLQETPAEAPAPVPPPAAVPPTASAPAATEAAAPAGAPAAAASAAEAPAVAASAAGAPAAAETPAAAESATEAPAAAESAAETPATAESAAEAPAAAESAAEAPAAAESAAEAPAAAESAAEAPAAAESAAEAPAAAEASAPAGAPAPAGALAGAQAPAPAGAPAGAQAPVEAQAEVPAQYPSERLIQSTSEENQIPSHLPVCPSLQHIARLRGRAIIDLFHSSIAEVENEELRHLLWSSVVFYQTPGLEVTACVLLSTKAVYFILHDGLRRYFSEPLQDFWHQKNTDYNNSPFHISQCFVLKLSDLQSVNVGLFDQYFRLTGSSPTQVVTCLTRDSYLTHCFLQHLMLVLSSLERTPSPEPIDKDFYSEFGDKNTGKMENYELIHSSRVKFTYPSEEEVGDLTYVVAQKMADPAKNPALSILLYIQAFQVITPQLGRGRGPLRPKTLLLTSAEIFLLDEDYIHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYNPYPQALTLVFDDTQGHDLMGSVTLDHFGEMPGGPGRAGQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

Target ORF information:

RefSeq Version	XM_017600295.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nischarin (Nisch), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600295.1

ORF Insert Sequence:

ATGGCGGCTG CGACACTCAG CTTCGGCCCT GAGCGAGAGG CCGAGCCTGC CAAGGAGGCG 
CGCGTTGTGG GTTCCGAGCT CGTGGACACG TACACGGTAT ATGTCATACA GGTTACCGAT 
GGCAACCATG AGTGGACGAT CAAACACCGC TATAGTGATT TTCATGACCT CCATGAAAAG 
CTTGTTGCTG AGAGAAAAAT TGACAAAACT CTACTTCCAC CCAAAAAGAT AATTGGAAAG 
AACTCGAGAA GCTTGGTAGA AAAGAGGGAG AAAGACCTGG AGGTGTACCT CCAGACTCTC 
CTGAAGACCT TCCCTGATGT GGCTCCCAGA GTTCTGGCCC ACTTCCTGCA TTTTCACCTC 
TATGAAATAA ATGGTGTCAC TGCAGCACTG GCTGAAGAAC TCTTTGAAAA AGGAGAACAG 
CTTCTAGGAG CCGGTGAGGT CTTTGCCATC AGGCCCTTGC AGCTCTATGC TATCACAGAA 
CAGCTGCAGC AGGGAAAGCC TACCTGTGCC AGCGGTGATG CCAAGACTGA CCTTGGACAC 
ATCCTAGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAGA AGGACCTTTT 
GGGACCAGCA ATATTCGAGA GCAGCTCCTG CCCTTTGATC TTTCGATATT CAAGTCTCTT 
CACCAGGTGG AGATGAGTCA CTGTGATGCC AAGCATGTCC GAGGGCTGGT CACCTCCAAG 
CCAACACTGG CCACAATGAG CGTTAGATTC TCAGCAGCCT CAATGAAGGA AGTTCTTGTT 
CCTGAAGCCT CAGAATTTGA TGAGTGGGAG CCTGAAGGCA CAACCCTCGG AGGCCCTGTG 
ACTGCTGTCA TCCCAACATG GCAAGCACTG ACCACTCTAG ACCTGAGCCA CAACAGCATC 
TCTGAGATCG ACGAATCTGT GAAACTGATC CCAAAGATTG AGTATCTGGA CCTGAGTCAC 
AATGGAGTGC TAGTTGTGGA CAACCTGCAG CACCTATACA ACCTCGTGCA CCTTGACCTG 
TCCTACAACA AGCTCTCCTC CCTGGAAGGC GTACACACTA AACTGGGCAA TGTCAAAACC 
CTAAACCTGG CAGGCAACTT CTTAGAGCGT CTGAGCGGTC TGCACAAACT CTATTCCCTG 
GTTAATCTGG ACCTCAGAGA CAACCGGATT GAGCAGTTGG ATGAAGTCAA GAGCATAGGC 
AACCTGCCGT GTCTGGAGCA CGTGGCCCTG CTAAACAACC CTCTGAGCAT CATCCCTGAC 
TACCGGACCA AGGTGCTTTC CCAGTTTGGA GAACGAGCCT CTGAGATCTG TCTAGATGAT 
GTCGCAACCA CAGAGAAGGA ACTGGACACT GTGGAAGTGC TAAAGGCCAT TCAGAAAGCC 
AAAGACGTCA AGTCCAAACT GAGCAGCACA GAAAAGAAGG TTGGTGAGGA CTTCCGGCTC 
CCAACTGCTC CCTGCATCAG ACCCAGCAGC TCCCCTCCCA CTGCCGTTCC CACCTCAGCC 
TCCCTGCCTC AGCCTATCCT CTCCAACCAA GGTATCATGT TCGTACAGGA AGAGGCCCTG 
GCCAGCAGCC TCTCATCCAC GGATAGCCTG CCACCTGATG ACCGGCCCAT TGCCCAAGCC 
TGCTCTAACT CCATGGGATC TCTCCCCACA GGACAGGTGG CCGCTGAAGA CTTAAGGGAC 
TTGCCAGGAG CCGTTGGCGG TGTGAGCCCA GATCATGCAG AACCAGAGGT TCAGGTGGTG 
CCTGGGTCCG GCCAGATCAT CTTCCTGCCC TTCACTTGCA TTGGCTACAC AGCCACCAAC 
CAGGACTTTA TCCAGCGACT CAGCACACTG ATCCGCCAGG CCATTGAACG GCAACTCCCT 
GCCTGGATTG AGGCTGCCAA CCAGCGAGAG GAAGCCCATG GTGAACAGGG GGAGGAAGAG 
GAGGAGGAGG AGGAAGAGGA TGTCGCTGAG AGCCGCTACT TTGAAATGGG ACCCCCAGAC 
GCAGAGGAAG AGGAGGGGAG TGGCCAGGGA GAAGAGGATG AGGAAGACGA GGACGAGGAG 
GCCGAGGAGG AGCGCCTGGC TCTGGAGTGG GCCCTGGGCG CTGATGAGGA CTTCCTGCTG 
GAGCACATCC GCATCCTCAA GGTGCTGTGG TGCTTCCTGA TCCACGTGCA AGGCAGCATC 
CGCCAGTTCG CTGCCTGCCT TGTGCTCACC GACTTCGGCA TCGCTGTCTT TGAGATCCCA 
CACCAAGAGT CCCGAGGCAG CAGCCAGCAC ATCCTCTCAT CCCTGCGCTT CGTCTTCTGC 
TTCCCGCACG GCGACCTCAC CGAGTTTGGC TTCCTCATGC CGGAGCTGTG TCTGGTGCTC 
AAGGTGCGGC ACAGTGAGAA CACGCTCTTC ATCATCTCAG ACGCTGCCAA CTTACACGAG 
TTCCATGCTG ATCTGCGCTC CTGCTTTGCG CCACAGCACA TGGCCATGCT GTGCAGCCCC 
ATCCTCTACG GCAGCCACAC CAGCCTGCAA GAGTTCTTGC GCCAGCTGCT CACCTTCTAC 
AAGGTGGCCG GTGGCTCTCA GGAGCGCAGC CAGGGCTGCT TCCCTGTCTA CCTGGTGTAC 
AGCGACAAGC GCATGGTGCA GACGGCTGCC GGGGACTACT CAGGCAACAT CGAGTGGGCC 
AGCTGCACGC TGTGCTCCGC AGTGCGGCGT TCCTGCTGCG CACCCTCTGA GGCCGTCAAG 
TCTGCCGCCA TCCCCTACTG GCTGCTGCTC ACCTCCCAGC ATCTCAACGT CATCAAGGCC 
GACTTCAACC CCATGCCCAG TCGTGGCACC CACAACTGTC GCAACCGCAA CAGCTTCAAG 
CTTAGCCGCG TCCCGCTCTC CACCGTGCTG CTGGACCCCA CTCGCAGCTG CACCCAGCCA 
CGGGGAGCCT TCGCCGATGG CCATGTGCTC GAGCTGCTTG TTGGCTACCG CTTTGTTACC 
GCCATCTTTG TGCTGCCCCA CGAGAAATTC CACTTCCTGC GAGTCTACAA TCAGCTCCGC 
GCCTCACTGA AGGACCTGAA GACTGTGGTC ATCGCCAAGA ATCCTTCCGC TAGGCCGAGA 
ACCCAAGGCC CCCTCGCGGG TGGCCAGCCT GCCAAAAGCA GGGTCAGTGC TGAGCAGCGG 
CTGCAGGAGA CCCCAGCAGA AGCTCCCGCT CCAGTTCCGC CTCCAGCTGC AGTCCCACCA 
ACAGCTTCAG CCCCAGCCGC AACAGAGGCT GCAGCTCCAG CAGGGGCTCC AGCCGCAGCC 
GCATCTGCAG CAGAGGCTCC AGCTGTAGCC GCATCTGCAG CAGGGGCTCC AGCCGCAGCA 
GAGACTCCAG CCGCAGCCGA ATCTGCAACA GAGGCTCCAG CCGCAGCTGA ATCTGCAGCA 
GAGACTCCAG CCACAGCCGA ATCTGCAGCA GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA 
GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA GAGGCTCCAG CCGCTGCCGA ATCTGCAGCA 
GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA GAGGCTCCAG CAGCGGCAGA GGCTTCAGCT 
CCAGCGGGGG CTCCGGCTCC AGCGGGGGCT CTGGCAGGGG CTCAGGCTCC GGCTCCAGCG 
GGGGCTCCGG CAGGGGCTCA GGCTCCAGTG GAGGCCCAAG CAGAGGTCCC TGCTCAGTAT 
CCAAGTGAGC GCCTAATCCA GTCCACGTCT GAAGAGAATC AGATCCCTTC TCACTTGCCA 
GTCTGCCCAT CACTCCAGCA CATCGCCCGT CTTCGGGGGC GCGCCATCAT TGACCTCTTC 
CACAGCAGCA TTGCCGAGGT TGAAAACGAG GAGCTAAGGC ACCTCCTGTG GTCGTCAGTG 
GTGTTCTACC AGACCCCGGG GCTGGAAGTG ACCGCCTGTG TGCTGCTGTC CACCAAGGCT 
GTGTACTTCA TACTGCATGA TGGCCTCCGC CGGTACTTCT CTGAACCGCT GCAGGATTTC 
TGGCACCAGA AAAACACTGA CTATAACAAC AGTCCTTTCC ACATCTCTCA GTGCTTTGTG 
CTCAAACTCA GTGACCTGCA GTCAGTCAAC GTCGGCCTTT TCGATCAGTA CTTCCGGCTG 
ACGGGCTCTT CCCCGACGCA GGTGGTCACA TGCTTGACTC GAGACAGCTA CCTGACACAC 
TGCTTCCTGC AGCACCTTAT GCTAGTGCTG TCCTCCCTGG AGCGCACACC TTCACCCGAG 
CCTATTGACA AGGACTTCTA CTCAGAATTT GGGGACAAGA ATACAGGGAA AATGGAGAAC 
TATGAGCTGA TCCATTCCAG CCGCGTCAAG TTCACCTACC CCAGTGAGGA AGAGGTCGGG 
GACCTGACCT ATGTTGTGGC ACAGAAGATG GCTGACCCTG CCAAGAATCC AGCCCTCAGC 
ATCTTATTGT ACATCCAGGC CTTCCAGGTG ATCACACCCC AGCTTGGGCG GGGCAGGGGC 
CCACTACGCC CTAAGACACT CCTACTGACC AGTGCTGAGA TCTTTCTCCT GGACGAGGAC 
TACATCCACT ATCCATTGCC TGAATTTGCC AAAGAGCCAC CACAGAGGGA CAGATACCGG 
CTAGACGATG GCCGCCGTGT CCGGGATTTA GACCGGGTGC TCATGGGCTA CAATCCCTAC 
CCACAGGCCC TCACTCTTGT TTTTGATGAC ACGCAGGGCC ACGACCTCAT GGGGAGTGTC 
ACCCTGGACC ACTTCGGGGA GATGCCGGGT GGCCCTGGTA GGGCTGGGCA GGGCCGGGAG 
GTGCAGTGGC AGGTGTTTGT GCCCAGTGCT GAGAGCCGAG AAAAGCTGAT CTCACTGCTC 
GCACGGCAGT GGGAAGCTCT CTGTGGCAGG GAGCTGCCTG TGGAGCTCAC TGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nisch ( XM_017587539.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017443028.1 Rattus norvegicus nischarin (Nisch), transcript variant X2, mRNA.

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CloneID	ORa54531
Clone ID Related Accession (Same CDS sequence)	XM_017600295.1 , XM_017587539.1
Accession Version	XM_017587539.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nischarin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000084.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGACACTCAG CTTCGGCCCT GAGCGAGAGG CCGAGCCTGC CAAGGAGGCG 
CGCGTTGTGG GTTCCGAGCT CGTGGACACG TACACGGTAT ATGTCATACA GGTTACCGAT 
GGCAACCATG AGTGGACGAT CAAACACCGC TATAGTGATT TTCATGACCT CCATGAAAAG 
CTTGTTGCTG AGAGAAAAAT TGACAAAACT CTACTTCCAC CCAAAAAGAT AATTGGAAAG 
AACTCGAGAA GCTTGGTAGA AAAGAGGGAG AAAGACCTGG AGGTGTACCT CCAGACTCTC 
CTGAAGACCT TCCCTGATGT GGCTCCCAGA GTTCTGGCCC ACTTCCTGCA TTTTCACCTC 
TATGAAATAA ATGGTGTCAC TGCAGCACTG GCTGAAGAAC TCTTTGAAAA AGGAGAACAG 
CTTCTAGGAG CCGGTGAGGT CTTTGCCATC AGGCCCTTGC AGCTCTATGC TATCACAGAA 
CAGCTGCAGC AGGGAAAGCC TACCTGTGCC AGCGGTGATG CCAAGACTGA CCTTGGACAC 
ATCCTAGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAGA AGGACCTTTT 
GGGACCAGCA ATATTCGAGA GCAGCTCCTG CCCTTTGATC TTTCGATATT CAAGTCTCTT 
CACCAGGTGG AGATGAGTCA CTGTGATGCC AAGCATGTCC GAGGGCTGGT CACCTCCAAG 
CCAACACTGG CCACAATGAG CGTTAGATTC TCAGCAGCCT CAATGAAGGA AGTTCTTGTT 
CCTGAAGCCT CAGAATTTGA TGAGTGGGAG CCTGAAGGCA CAACCCTCGG AGGCCCTGTG 
ACTGCTGTCA TCCCAACATG GCAAGCACTG ACCACTCTAG ACCTGAGCCA CAACAGCATC 
TCTGAGATCG ACGAATCTGT GAAACTGATC CCAAAGATTG AGTATCTGGA CCTGAGTCAC 
AATGGAGTGC TAGTTGTGGA CAACCTGCAG CACCTATACA ACCTCGTGCA CCTTGACCTG 
TCCTACAACA AGCTCTCCTC CCTGGAAGGC GTACACACTA AACTGGGCAA TGTCAAAACC 
CTAAACCTGG CAGGCAACTT CTTAGAGCGT CTGAGCGGTC TGCACAAACT CTATTCCCTG 
GTTAATCTGG ACCTCAGAGA CAACCGGATT GAGCAGTTGG ATGAAGTCAA GAGCATAGGC 
AACCTGCCGT GTCTGGAGCA CGTGGCCCTG CTAAACAACC CTCTGAGCAT CATCCCTGAC 
TACCGGACCA AGGTGCTTTC CCAGTTTGGA GAACGAGCCT CTGAGATCTG TCTAGATGAT 
GTCGCAACCA CAGAGAAGGA ACTGGACACT GTGGAAGTGC TAAAGGCCAT TCAGAAAGCC 
AAAGACGTCA AGTCCAAACT GAGCAGCACA GAAAAGAAGG TTGGTGAGGA CTTCCGGCTC 
CCAACTGCTC CCTGCATCAG ACCCAGCAGC TCCCCTCCCA CTGCCGTTCC CACCTCAGCC 
TCCCTGCCTC AGCCTATCCT CTCCAACCAA GGTATCATGT TCGTACAGGA AGAGGCCCTG 
GCCAGCAGCC TCTCATCCAC GGATAGCCTG CCACCTGATG ACCGGCCCAT TGCCCAAGCC 
TGCTCTAACT CCATGGGATC TCTCCCCACA GGACAGGTGG CCGCTGAAGA CTTAAGGGAC 
TTGCCAGGAG CCGTTGGCGG TGTGAGCCCA GATCATGCAG AACCAGAGGT TCAGGTGGTG 
CCTGGGTCCG GCCAGATCAT CTTCCTGCCC TTCACTTGCA TTGGCTACAC AGCCACCAAC 
CAGGACTTTA TCCAGCGACT CAGCACACTG ATCCGCCAGG CCATTGAACG GCAACTCCCT 
GCCTGGATTG AGGCTGCCAA CCAGCGAGAG GAAGCCCATG GTGAACAGGG GGAGGAAGAG 
GAGGAGGAGG AGGAAGAGGA TGTCGCTGAG AGCCGCTACT TTGAAATGGG ACCCCCAGAC 
GCAGAGGAAG AGGAGGGGAG TGGCCAGGGA GAAGAGGATG AGGAAGACGA GGACGAGGAG 
GCCGAGGAGG AGCGCCTGGC TCTGGAGTGG GCCCTGGGCG CTGATGAGGA CTTCCTGCTG 
GAGCACATCC GCATCCTCAA GGTGCTGTGG TGCTTCCTGA TCCACGTGCA AGGCAGCATC 
CGCCAGTTCG CTGCCTGCCT TGTGCTCACC GACTTCGGCA TCGCTGTCTT TGAGATCCCA 
CACCAAGAGT CCCGAGGCAG CAGCCAGCAC ATCCTCTCAT CCCTGCGCTT CGTCTTCTGC 
TTCCCGCACG GCGACCTCAC CGAGTTTGGC TTCCTCATGC CGGAGCTGTG TCTGGTGCTC 
AAGGTGCGGC ACAGTGAGAA CACGCTCTTC ATCATCTCAG ACGCTGCCAA CTTACACGAG 
TTCCATGCTG ATCTGCGCTC CTGCTTTGCG CCACAGCACA TGGCCATGCT GTGCAGCCCC 
ATCCTCTACG GCAGCCACAC CAGCCTGCAA GAGTTCTTGC GCCAGCTGCT CACCTTCTAC 
AAGGTGGCCG GTGGCTCTCA GGAGCGCAGC CAGGGCTGCT TCCCTGTCTA CCTGGTGTAC 
AGCGACAAGC GCATGGTGCA GACGGCTGCC GGGGACTACT CAGGCAACAT CGAGTGGGCC 
AGCTGCACGC TGTGCTCCGC AGTGCGGCGT TCCTGCTGCG CACCCTCTGA GGCCGTCAAG 
TCTGCCGCCA TCCCCTACTG GCTGCTGCTC ACCTCCCAGC ATCTCAACGT CATCAAGGCC 
GACTTCAACC CCATGCCCAG TCGTGGCACC CACAACTGTC GCAACCGCAA CAGCTTCAAG 
CTTAGCCGCG TCCCGCTCTC CACCGTGCTG CTGGACCCCA CTCGCAGCTG CACCCAGCCA 
CGGGGAGCCT TCGCCGATGG CCATGTGCTC GAGCTGCTTG TTGGCTACCG CTTTGTTACC 
GCCATCTTTG TGCTGCCCCA CGAGAAATTC CACTTCCTGC GAGTCTACAA TCAGCTCCGC 
GCCTCACTGA AGGACCTGAA GACTGTGGTC ATCGCCAAGA ATCCTTCCGC TAGGCCGAGA 
ACCCAAGGCC CCCTCGCGGG TGGCCAGCCT GCCAAAAGCA GGGTCAGTGC TGAGCAGCGG 
CTGCAGGAGA CCCCAGCAGA AGCTCCCGCT CCAGTTCCGC CTCCAGCTGC AGTCCCACCA 
ACAGCTTCAG CCCCAGCCGC AACAGAGGCT GCAGCTCCAG CAGGGGCTCC AGCCGCAGCC 
GCATCTGCAG CAGAGGCTCC AGCTGTAGCC GCATCTGCAG CAGGGGCTCC AGCCGCAGCA 
GAGACTCCAG CCGCAGCCGA ATCTGCAACA GAGGCTCCAG CCGCAGCTGA ATCTGCAGCA 
GAGACTCCAG CCACAGCCGA ATCTGCAGCA GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA 
GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA GAGGCTCCAG CCGCTGCCGA ATCTGCAGCA 
GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA GAGGCTCCAG CAGCGGCAGA GGCTTCAGCT 
CCAGCGGGGG CTCCGGCTCC AGCGGGGGCT CTGGCAGGGG CTCAGGCTCC GGCTCCAGCG 
GGGGCTCCGG CAGGGGCTCA GGCTCCAGTG GAGGCCCAAG CAGAGGTCCC TGCTCAGTAT 
CCAAGTGAGC GCCTAATCCA GTCCACGTCT GAAGAGAATC AGATCCCTTC TCACTTGCCA 
GTCTGCCCAT CACTCCAGCA CATCGCCCGT CTTCGGGGGC GCGCCATCAT TGACCTCTTC 
CACAGCAGCA TTGCCGAGGT TGAAAACGAG GAGCTAAGGC ACCTCCTGTG GTCGTCAGTG 
GTGTTCTACC AGACCCCGGG GCTGGAAGTG ACCGCCTGTG TGCTGCTGTC CACCAAGGCT 
GTGTACTTCA TACTGCATGA TGGCCTCCGC CGGTACTTCT CTGAACCGCT GCAGGATTTC 
TGGCACCAGA AAAACACTGA CTATAACAAC AGTCCTTTCC ACATCTCTCA GTGCTTTGTG 
CTCAAACTCA GTGACCTGCA GTCAGTCAAC GTCGGCCTTT TCGATCAGTA CTTCCGGCTG 
ACGGGCTCTT CCCCGACGCA GGTGGTCACA TGCTTGACTC GAGACAGCTA CCTGACACAC 
TGCTTCCTGC AGCACCTTAT GCTAGTGCTG TCCTCCCTGG AGCGCACACC TTCACCCGAG 
CCTATTGACA AGGACTTCTA CTCAGAATTT GGGGACAAGA ATACAGGGAA AATGGAGAAC 
TATGAGCTGA TCCATTCCAG CCGCGTCAAG TTCACCTACC CCAGTGAGGA AGAGGTCGGG 
GACCTGACCT ATGTTGTGGC ACAGAAGATG GCTGACCCTG CCAAGAATCC AGCCCTCAGC 
ATCTTATTGT ACATCCAGGC CTTCCAGGTG ATCACACCCC AGCTTGGGCG GGGCAGGGGC 
CCACTACGCC CTAAGACACT CCTACTGACC AGTGCTGAGA TCTTTCTCCT GGACGAGGAC 
TACATCCACT ATCCATTGCC TGAATTTGCC AAAGAGCCAC CACAGAGGGA CAGATACCGG 
CTAGACGATG GCCGCCGTGT CCGGGATTTA GACCGGGTGC TCATGGGCTA CAATCCCTAC 
CCACAGGCCC TCACTCTTGT TTTTGATGAC ACGCAGGGCC ACGACCTCAT GGGGAGTGTC 
ACCCTGGACC ACTTCGGGGA GATGCCGGGT GGCCCTGGTA GGGCTGGGCA GGGCCGGGAG 
GTGCAGTGGC AGGTGTTTGT GCCCAGTGCT GAGAGCCGAG AAAAGCTGAT CTCACTGCTC 
GCACGGCAGT GGGAAGCTCT CTGTGGCAGG GAGCTGCCTG TGGAGCTCAC TGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017443028.1
CDS	111..4967
Translation	MAAATLSFGPEREAEPAKEARVVGSELVDTYTVYVIQVTDGNHEWTIKHRYSDFHDLHEKLVAERKIDKTLLPPKKIIGKNSRSLVEKREKDLEVYLQTLLKTFPDVAPRVLAHFLHFHLYEINGVTAALAEELFEKGEQLLGAGEVFAIRPLQLYAITEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIREQLLPFDLSIFKSLHQVEMSHCDAKHVRGLVTSKPTLATMSVRFSAASMKEVLVPEASEFDEWEPEGTTLGGPVTAVIPTWQALTTLDLSHNSISEIDESVKLIPKIEYLDLSHNGVLVVDNLQHLYNLVHLDLSYNKLSSLEGVHTKLGNVKTLNLAGNFLERLSGLHKLYSLVNLDLRDNRIEQLDEVKSIGNLPCLEHVALLNNPLSIIPDYRTKVLSQFGERASEICLDDVATTEKELDTVEVLKAIQKAKDVKSKLSSTEKKVGEDFRLPTAPCIRPSSSPPTAVPTSASLPQPILSNQGIMFVQEEALASSLSSTDSLPPDDRPIAQACSNSMGSLPTGQVAAEDLRDLPGAVGGVSPDHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREEAHGEQGEEEEEEEEEDVAESRYFEMGPPDAEEEEGSGQGEEDEEDEDEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTSLQEFLRQLLTFYKVAGGSQERSQGCFPVYLVYSDKRMVQTAAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTSQHLNVIKADFNPMPSRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLKDLKTVVIAKNPSARPRTQGPLAGGQPAKSRVSAEQRLQETPAEAPAPVPPPAAVPPTASAPAATEAAAPAGAPAAAASAAEAPAVAASAAGAPAAAETPAAAESATEAPAAAESAAETPATAESAAEAPAAAESAAEAPAAAESAAEAPAAAESAAEAPAAAESAAEAPAAAEASAPAGAPAPAGALAGAQAPAPAGAPAGAQAPVEAQAEVPAQYPSERLIQSTSEENQIPSHLPVCPSLQHIARLRGRAIIDLFHSSIAEVENEELRHLLWSSVVFYQTPGLEVTACVLLSTKAVYFILHDGLRRYFSEPLQDFWHQKNTDYNNSPFHISQCFVLKLSDLQSVNVGLFDQYFRLTGSSPTQVVTCLTRDSYLTHCFLQHLMLVLSSLERTPSPEPIDKDFYSEFGDKNTGKMENYELIHSSRVKFTYPSEEEVGDLTYVVAQKMADPAKNPALSILLYIQAFQVITPQLGRGRGPLRPKTLLLTSAEIFLLDEDYIHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYNPYPQALTLVFDDTQGHDLMGSVTLDHFGEMPGGPGRAGQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

Target ORF information:

RefSeq Version	XM_017587539.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nischarin (Nisch), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017587539.1

ORF Insert Sequence:

ATGGCGGCTG CGACACTCAG CTTCGGCCCT GAGCGAGAGG CCGAGCCTGC CAAGGAGGCG 
CGCGTTGTGG GTTCCGAGCT CGTGGACACG TACACGGTAT ATGTCATACA GGTTACCGAT 
GGCAACCATG AGTGGACGAT CAAACACCGC TATAGTGATT TTCATGACCT CCATGAAAAG 
CTTGTTGCTG AGAGAAAAAT TGACAAAACT CTACTTCCAC CCAAAAAGAT AATTGGAAAG 
AACTCGAGAA GCTTGGTAGA AAAGAGGGAG AAAGACCTGG AGGTGTACCT CCAGACTCTC 
CTGAAGACCT TCCCTGATGT GGCTCCCAGA GTTCTGGCCC ACTTCCTGCA TTTTCACCTC 
TATGAAATAA ATGGTGTCAC TGCAGCACTG GCTGAAGAAC TCTTTGAAAA AGGAGAACAG 
CTTCTAGGAG CCGGTGAGGT CTTTGCCATC AGGCCCTTGC AGCTCTATGC TATCACAGAA 
CAGCTGCAGC AGGGAAAGCC TACCTGTGCC AGCGGTGATG CCAAGACTGA CCTTGGACAC 
ATCCTAGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAGA AGGACCTTTT 
GGGACCAGCA ATATTCGAGA GCAGCTCCTG CCCTTTGATC TTTCGATATT CAAGTCTCTT 
CACCAGGTGG AGATGAGTCA CTGTGATGCC AAGCATGTCC GAGGGCTGGT CACCTCCAAG 
CCAACACTGG CCACAATGAG CGTTAGATTC TCAGCAGCCT CAATGAAGGA AGTTCTTGTT 
CCTGAAGCCT CAGAATTTGA TGAGTGGGAG CCTGAAGGCA CAACCCTCGG AGGCCCTGTG 
ACTGCTGTCA TCCCAACATG GCAAGCACTG ACCACTCTAG ACCTGAGCCA CAACAGCATC 
TCTGAGATCG ACGAATCTGT GAAACTGATC CCAAAGATTG AGTATCTGGA CCTGAGTCAC 
AATGGAGTGC TAGTTGTGGA CAACCTGCAG CACCTATACA ACCTCGTGCA CCTTGACCTG 
TCCTACAACA AGCTCTCCTC CCTGGAAGGC GTACACACTA AACTGGGCAA TGTCAAAACC 
CTAAACCTGG CAGGCAACTT CTTAGAGCGT CTGAGCGGTC TGCACAAACT CTATTCCCTG 
GTTAATCTGG ACCTCAGAGA CAACCGGATT GAGCAGTTGG ATGAAGTCAA GAGCATAGGC 
AACCTGCCGT GTCTGGAGCA CGTGGCCCTG CTAAACAACC CTCTGAGCAT CATCCCTGAC 
TACCGGACCA AGGTGCTTTC CCAGTTTGGA GAACGAGCCT CTGAGATCTG TCTAGATGAT 
GTCGCAACCA CAGAGAAGGA ACTGGACACT GTGGAAGTGC TAAAGGCCAT TCAGAAAGCC 
AAAGACGTCA AGTCCAAACT GAGCAGCACA GAAAAGAAGG TTGGTGAGGA CTTCCGGCTC 
CCAACTGCTC CCTGCATCAG ACCCAGCAGC TCCCCTCCCA CTGCCGTTCC CACCTCAGCC 
TCCCTGCCTC AGCCTATCCT CTCCAACCAA GGTATCATGT TCGTACAGGA AGAGGCCCTG 
GCCAGCAGCC TCTCATCCAC GGATAGCCTG CCACCTGATG ACCGGCCCAT TGCCCAAGCC 
TGCTCTAACT CCATGGGATC TCTCCCCACA GGACAGGTGG CCGCTGAAGA CTTAAGGGAC 
TTGCCAGGAG CCGTTGGCGG TGTGAGCCCA GATCATGCAG AACCAGAGGT TCAGGTGGTG 
CCTGGGTCCG GCCAGATCAT CTTCCTGCCC TTCACTTGCA TTGGCTACAC AGCCACCAAC 
CAGGACTTTA TCCAGCGACT CAGCACACTG ATCCGCCAGG CCATTGAACG GCAACTCCCT 
GCCTGGATTG AGGCTGCCAA CCAGCGAGAG GAAGCCCATG GTGAACAGGG GGAGGAAGAG 
GAGGAGGAGG AGGAAGAGGA TGTCGCTGAG AGCCGCTACT TTGAAATGGG ACCCCCAGAC 
GCAGAGGAAG AGGAGGGGAG TGGCCAGGGA GAAGAGGATG AGGAAGACGA GGACGAGGAG 
GCCGAGGAGG AGCGCCTGGC TCTGGAGTGG GCCCTGGGCG CTGATGAGGA CTTCCTGCTG 
GAGCACATCC GCATCCTCAA GGTGCTGTGG TGCTTCCTGA TCCACGTGCA AGGCAGCATC 
CGCCAGTTCG CTGCCTGCCT TGTGCTCACC GACTTCGGCA TCGCTGTCTT TGAGATCCCA 
CACCAAGAGT CCCGAGGCAG CAGCCAGCAC ATCCTCTCAT CCCTGCGCTT CGTCTTCTGC 
TTCCCGCACG GCGACCTCAC CGAGTTTGGC TTCCTCATGC CGGAGCTGTG TCTGGTGCTC 
AAGGTGCGGC ACAGTGAGAA CACGCTCTTC ATCATCTCAG ACGCTGCCAA CTTACACGAG 
TTCCATGCTG ATCTGCGCTC CTGCTTTGCG CCACAGCACA TGGCCATGCT GTGCAGCCCC 
ATCCTCTACG GCAGCCACAC CAGCCTGCAA GAGTTCTTGC GCCAGCTGCT CACCTTCTAC 
AAGGTGGCCG GTGGCTCTCA GGAGCGCAGC CAGGGCTGCT TCCCTGTCTA CCTGGTGTAC 
AGCGACAAGC GCATGGTGCA GACGGCTGCC GGGGACTACT CAGGCAACAT CGAGTGGGCC 
AGCTGCACGC TGTGCTCCGC AGTGCGGCGT TCCTGCTGCG CACCCTCTGA GGCCGTCAAG 
TCTGCCGCCA TCCCCTACTG GCTGCTGCTC ACCTCCCAGC ATCTCAACGT CATCAAGGCC 
GACTTCAACC CCATGCCCAG TCGTGGCACC CACAACTGTC GCAACCGCAA CAGCTTCAAG 
CTTAGCCGCG TCCCGCTCTC CACCGTGCTG CTGGACCCCA CTCGCAGCTG CACCCAGCCA 
CGGGGAGCCT TCGCCGATGG CCATGTGCTC GAGCTGCTTG TTGGCTACCG CTTTGTTACC 
GCCATCTTTG TGCTGCCCCA CGAGAAATTC CACTTCCTGC GAGTCTACAA TCAGCTCCGC 
GCCTCACTGA AGGACCTGAA GACTGTGGTC ATCGCCAAGA ATCCTTCCGC TAGGCCGAGA 
ACCCAAGGCC CCCTCGCGGG TGGCCAGCCT GCCAAAAGCA GGGTCAGTGC TGAGCAGCGG 
CTGCAGGAGA CCCCAGCAGA AGCTCCCGCT CCAGTTCCGC CTCCAGCTGC AGTCCCACCA 
ACAGCTTCAG CCCCAGCCGC AACAGAGGCT GCAGCTCCAG CAGGGGCTCC AGCCGCAGCC 
GCATCTGCAG CAGAGGCTCC AGCTGTAGCC GCATCTGCAG CAGGGGCTCC AGCCGCAGCA 
GAGACTCCAG CCGCAGCCGA ATCTGCAACA GAGGCTCCAG CCGCAGCTGA ATCTGCAGCA 
GAGACTCCAG CCACAGCCGA ATCTGCAGCA GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA 
GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA GAGGCTCCAG CCGCTGCCGA ATCTGCAGCA 
GAGGCTCCAG CCGCAGCCGA ATCTGCAGCA GAGGCTCCAG CAGCGGCAGA GGCTTCAGCT 
CCAGCGGGGG CTCCGGCTCC AGCGGGGGCT CTGGCAGGGG CTCAGGCTCC GGCTCCAGCG 
GGGGCTCCGG CAGGGGCTCA GGCTCCAGTG GAGGCCCAAG CAGAGGTCCC TGCTCAGTAT 
CCAAGTGAGC GCCTAATCCA GTCCACGTCT GAAGAGAATC AGATCCCTTC TCACTTGCCA 
GTCTGCCCAT CACTCCAGCA CATCGCCCGT CTTCGGGGGC GCGCCATCAT TGACCTCTTC 
CACAGCAGCA TTGCCGAGGT TGAAAACGAG GAGCTAAGGC ACCTCCTGTG GTCGTCAGTG 
GTGTTCTACC AGACCCCGGG GCTGGAAGTG ACCGCCTGTG TGCTGCTGTC CACCAAGGCT 
GTGTACTTCA TACTGCATGA TGGCCTCCGC CGGTACTTCT CTGAACCGCT GCAGGATTTC 
TGGCACCAGA AAAACACTGA CTATAACAAC AGTCCTTTCC ACATCTCTCA GTGCTTTGTG 
CTCAAACTCA GTGACCTGCA GTCAGTCAAC GTCGGCCTTT TCGATCAGTA CTTCCGGCTG 
ACGGGCTCTT CCCCGACGCA GGTGGTCACA TGCTTGACTC GAGACAGCTA CCTGACACAC 
TGCTTCCTGC AGCACCTTAT GCTAGTGCTG TCCTCCCTGG AGCGCACACC TTCACCCGAG 
CCTATTGACA AGGACTTCTA CTCAGAATTT GGGGACAAGA ATACAGGGAA AATGGAGAAC 
TATGAGCTGA TCCATTCCAG CCGCGTCAAG TTCACCTACC CCAGTGAGGA AGAGGTCGGG 
GACCTGACCT ATGTTGTGGC ACAGAAGATG GCTGACCCTG CCAAGAATCC AGCCCTCAGC 
ATCTTATTGT ACATCCAGGC CTTCCAGGTG ATCACACCCC AGCTTGGGCG GGGCAGGGGC 
CCACTACGCC CTAAGACACT CCTACTGACC AGTGCTGAGA TCTTTCTCCT GGACGAGGAC 
TACATCCACT ATCCATTGCC TGAATTTGCC AAAGAGCCAC CACAGAGGGA CAGATACCGG 
CTAGACGATG GCCGCCGTGT CCGGGATTTA GACCGGGTGC TCATGGGCTA CAATCCCTAC 
CCACAGGCCC TCACTCTTGT TTTTGATGAC ACGCAGGGCC ACGACCTCAT GGGGAGTGTC 
ACCCTGGACC ACTTCGGGGA GATGCCGGGT GGCCCTGGTA GGGCTGGGCA GGGCCGGGAG 
GTGCAGTGGC AGGTGTTTGT GCCCAGTGCT GAGAGCCGAG AAAAGCTGAT CTCACTGCTC 
GCACGGCAGT GGGAAGCTCT CTGTGGCAGG GAGCTGCCTG TGGAGCTCAC TGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inhibition of Nischarin Expression Promotes Neurite Outgrowth through Regulation of PAK Activity.
PloS one10(12)e0144948(2015)
Ding Y,Li Y,Lu L,Zhang R,Zeng L,Wang L,Zhang X

The expression pattern of Nischarin after lipopolysaccharides (LPS)-induced neuroinflammation in rats brain cortex.
Inflammation research : official journal of the European Histamine Research Society ... [et al.]62(11)929-40(2013 Nov)
Wu X,Xu W,Cui G,Yan Y,Wu X,Li L,Tan X,Wu Q,Gu X

Rac and Rab GTPases dual effector Nischarin regulates vesicle maturation to facilitate survival of intracellular bacteria.
The EMBO journal32(5)713-27(2013 Mar)
Kuijl C,Pilli M,Alahari SK,Janssen H,Khoo PS,Ervin KE,Calero M,Jonnalagadda S,Scheller RH,Neefjes J,Junutula JR

Nischarin is differentially expressed in rat brain and regulates neuronal migration.
PloS one8(1)e54563(2013)
Ding Y,Zhang R,Zhang K,Lv X,Chen Y,Li A,Wang L,Zhang X,Xia Q

I1 imidazoline receptor: novel potential cytoprotective target of TVP1022, the S-enantiomer of rasagiline.
PloS one7(11)e47890(2012)
Barac YD,Bar-Am O,Liani E,Amit T,Frolov L,Ovcharenko E,Angel I,Youdim MB,Binah O

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Nisch cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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