NISCH gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_007184.3	NP_009115.2	nischarin isoform 1
XM_017005643.1	XP_016861132.1	nischarin isoform X1
XM_005264839.2	XP_005264896.1	nischarin isoform X1
NM_001276294.2	NP_001263223.2	nischarin isoform 3
XM_006712955.2	XP_006713018.1	nischarin isoform X2
XM_006712955.3	XP_006713018.1	nischarin isoform X2
XM_017005643.2	XP_016861132.1	nischarin isoform X1
NM_001276293.1	NP_001263222.1	nischarin isoform 2
NM_001276294.1	NP_001263223.1	nischarin isoform 3
XM_005264839.3	XP_005264896.1	nischarin isoform X1
NM_001276293.2	NP_001263222.2	nischarin isoform 2
NM_007184.4	NP_009115.3	nischarin isoform 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14479	NM_001276293.1 Latest version!	Homo sapiens nischarin (NISCH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu14575	NM_001276294.1 Latest version!	Homo sapiens nischarin (NISCH), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu108698	NM_001276294.2 Latest version!	Homo sapiens nischarin (NISCH), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu108703	NM_001276293.2 Latest version!	Homo sapiens nischarin (NISCH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu107681	NM_007184.4 Latest version!	Homo sapiens nischarin (NISCH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu19507	NM_007184.3 Latest version!	Homo sapiens nischarin (NISCH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
OHu36939	XM_005264839.3 Latest version!	Homo sapiens nischarin (NISCH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu36939	XM_017005643.2 Latest version!	Homo sapiens nischarin (NISCH), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu36940	XM_006712955.3 Latest version!	Homo sapiens nischarin (NISCH), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OHu36940	XM_006712955.2	Homo sapiens nischarin (NISCH), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu36939	XM_017005643.1	Homo sapiens nischarin (NISCH), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu36939	XM_005264839.2	Homo sapiens nischarin (NISCH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
Hide Old Accession Versions >>

NISCH ( NM_001276293.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001263222.1 Homo sapiens nischarin (NISCH), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu14479
Clone ID Related Accession (Same CDS sequence)	NM_001276293.1
Accession Version	NM_001276293.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	nischarin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006208.3, AL703940.1, BC050552.1 and BC038102.2. Transcript Variant: This variant (2) has its transcription extend past a splice site that is used in variant 1, resulting in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2 (also known as IRAS-L) which is shorter and has a distinct C-terminus, compared to isoform 1. This isoform lacks multiple domains which are believed to be required by functional imidazoline receptors. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050552.1, SRR1660805.61416.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCGACCG CGCGCACCTT CGGGCCCGAG CGGGAAGCCG AGCCGGCCAA GGAAGCGCGC 
GTCGTGGGCT CGGAGCTTGT GGACACTTAT ACGGTTTACA TCATCCAGGT CACTGATGGC 
AGCCATGAGT GGACAGTAAA GCACCGCTAC AGCGACTTCC ATGACCTGCA TGAAAAGCTC 
GTTGCAGAGA GAAAGATTGA TAAAAATCTG CTTCCGCCCA AAAAGATAAT TGGGAAAAAC 
TCAAGAAGCT TGGTGGAGAA GAGGGAGAAG GATCTGGAGG TCTACCTCCA GAAGCTCCTG 
GCTGCCTTCC CTGGCGTGAC CCCCAGAGTA CTGGCCCACT TCTTGCATTT TCACTTCTAT 
GAGATAAATG GCATCACCGC GGCACTGGCT GAAGAGCTCT TTGAGAAAGG AGAACAGCTC 
CTGGGGGCCG GCGAGGTCTT TGCCATTGGA CCCCTGCAGC TGTATGCCGT CACGGAGCAG 
CTGCAGCAGG GAAAGCCCAC GTGCGCCAGT GGGGATGCCA AGACCGACCT CGGGCACATC 
CTGGACTTCA CCTGTCGCCT TAAGTACCTT AAGGTTTCTG GCACAGAAGG ACCTTTTGGG 
ACCAGCAACA TTCAGGAGCA GCTCCTGCCG TTCGACCTAT CAATATTCAA GTCCCTGCAT 
CAGGTGGAGA TAAGTCACTG TGATGCTAAG CACATCAGAG GGCTGGTCGC ATCGAAGCCC 
ACCTTAGCCA CGCTGAGTGT CCGCTTCTCA GCAACCTCGA TGAAGGAAGT CCTTGTTCCT 
GAAGCCTCAG AATTTGATGA GTGGGAGCCT GAAGGCACAA CCCTAGAAGG CCCTGTGACT 
GCCGTCATCC CCACTTGGCA GGCATTGACC ACGCTTGACC TGAGCCACAA CAGCATCTCC 
GAGATCGACG AGTCTGTGAA ACTGATCCCA AAGATTGAGT TCCTGGACCT GAGTCACAAT 
GGATTGCTGG TTGTGGACAA TCTGCAGCAC CTGTATAACC TTGTGCATCT GGACCTGTCC 
TACAACAAGC TCTCCTCCTT GGAAGGGCTT CACACCAAGC TGGGGAACAT CAAGACCTTA 
AACCTGGCAG GCAACCTCCT AGAGAGTCTG AGTGGCCTGC ACAAGCTCTA CTCACTGGTC 
AACCTGGATC TCCGGGACAA CAGGATCGAA CAGATGGAGG AGGTCCGGAG CATAGGCAGC 
CTCCCGTGTC TGGAGCACGT GTCTCTGCTG AACAACCCTC TGAGCATCAT CCCCGACTAC 
CGGACCAAGG TGCTGGCTCA GTTCGGAGAG AGGGCCTCAG AGGTCTGTCT GGATGACACA 
GTGACCACAG AGAAGGAGCT GGACACTGTG GAAGTGCTGA AAGCAATTCA GAAAGCCAAG 
GAGGTCAAGT CCAAACTGAG CAACCCAGAG AAGAAGGGTG GTGAAGACTC CCGGCTCTCA 
GCTGCCCCCT GCATCAGACC CAGCAGCTCC CCTCCCACTG TGGCTCCCGC ATCTGCCTCC 
CTGCCCCAGC CCATCCTCTC TAACCAAGGT AATCGTGTAT GTATCTTGCT TCTAGTGGAG 
CCACACAGCC CTGCCTGGGC CCCCTGGCTG GGCTGGGGTT GGGGGAGAGG TGCCAGCACC 
TGCTTCCAAC AGGGTCAGAC ACAGGGAGGG CAGTGCCTTC TGCAGGCTGG TCCTCGCGGG 
GGGACACATG GCAGGGGTGC CTGGCCTGAT GCCAGCTGTT GCTTGCTTGG TGAGGACTCC 
CAATTGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263222.1
CDS	135..1886
Translation	MATARTFGPEREAEPAKEARVVGSELVDTYTVYIIQVTDGSHEWTVKHRYSDFHDLHEKLVAERKIDKNLLPPKKIIGKNSRSLVEKREKDLEVYLQKLLAAFPGVTPRVLAHFLHFHFYEINGITAALAEELFEKGEQLLGAGEVFAIGPLQLYAVTEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIQEQLLPFDLSIFKSLHQVEISHCDAKHIRGLVASKPTLATLSVRFSATSMKEVLVPEASEFDEWEPEGTTLEGPVTAVIPTWQALTTLDLSHNSISEIDESVKLIPKIEFLDLSHNGLLVVDNLQHLYNLVHLDLSYNKLSSLEGLHTKLGNIKTLNLAGNLLESLSGLHKLYSLVNLDLRDNRIEQMEEVRSIGSLPCLEHVSLLNNPLSIIPDYRTKVLAQFGERASEVCLDDTVTTEKELDTVEVLKAIQKAKEVKSKLSNPEKKGGEDSRLSAAPCIRPSSSPPTVAPASASLPQPILSNQGNRVCILLLVEPHSPAWAPWLGWGWGRGASTCFQQGQTQGGQCLLQAGPRGGTHGRGAWPDASCCLLGEDSQLL

Target ORF information:

RefSeq Version	NM_001276293.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nischarin (NISCH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276293.1

ORF Insert Sequence:

ATGGCGACCG CGCGCACCTT CGGGCCCGAG CGGGAAGCCG AGCCGGCCAA GGAAGCGCGC 
GTCGTGGGCT CGGAGCTTGT GGACACTTAT ACGGTTTACA TCATCCAGGT CACTGATGGC 
AGCCATGAGT GGACAGTAAA GCACCGCTAC AGCGACTTCC ATGACCTGCA TGAAAAGCTC 
GTTGCAGAGA GAAAGATTGA TAAAAATCTG CTTCCGCCCA AAAAGATAAT TGGGAAAAAC 
TCAAGAAGCT TGGTGGAGAA GAGGGAGAAG GATCTGGAGG TCTACCTCCA GAAGCTCCTG 
GCTGCCTTCC CTGGCGTGAC CCCCAGAGTA CTGGCCCACT TCTTGCATTT TCACTTCTAT 
GAGATAAATG GCATCACCGC GGCACTGGCT GAAGAGCTCT TTGAGAAAGG AGAACAGCTC 
CTGGGGGCCG GCGAGGTCTT TGCCATTGGA CCCCTGCAGC TGTATGCCGT CACGGAGCAG 
CTGCAGCAGG GAAAGCCCAC GTGCGCCAGT GGGGATGCCA AGACCGACCT CGGGCACATC 
CTGGACTTCA CCTGTCGCCT TAAGTACCTT AAGGTTTCTG GCACAGAAGG ACCTTTTGGG 
ACCAGCAACA TTCAGGAGCA GCTCCTGCCG TTCGACCTAT CAATATTCAA GTCCCTGCAT 
CAGGTGGAGA TAAGTCACTG TGATGCTAAG CACATCAGAG GGCTGGTCGC ATCGAAGCCC 
ACCTTAGCCA CGCTGAGTGT CCGCTTCTCA GCAACCTCGA TGAAGGAAGT CCTTGTTCCT 
GAAGCCTCAG AATTTGATGA GTGGGAGCCT GAAGGCACAA CCCTAGAAGG CCCTGTGACT 
GCCGTCATCC CCACTTGGCA GGCATTGACC ACGCTTGACC TGAGCCACAA CAGCATCTCC 
GAGATCGACG AGTCTGTGAA ACTGATCCCA AAGATTGAGT TCCTGGACCT GAGTCACAAT 
GGATTGCTGG TTGTGGACAA TCTGCAGCAC CTGTATAACC TTGTGCATCT GGACCTGTCC 
TACAACAAGC TCTCCTCCTT GGAAGGGCTT CACACCAAGC TGGGGAACAT CAAGACCTTA 
AACCTGGCAG GCAACCTCCT AGAGAGTCTG AGTGGCCTGC ACAAGCTCTA CTCACTGGTC 
AACCTGGATC TCCGGGACAA CAGGATCGAA CAGATGGAGG AGGTCCGGAG CATAGGCAGC 
CTCCCGTGTC TGGAGCACGT GTCTCTGCTG AACAACCCTC TGAGCATCAT CCCCGACTAC 
CGGACCAAGG TGCTGGCTCA GTTCGGAGAG AGGGCCTCAG AGGTCTGTCT GGATGACACA 
GTGACCACAG AGAAGGAGCT GGACACTGTG GAAGTGCTGA AAGCAATTCA GAAAGCCAAG 
GAGGTCAAGT CCAAACTGAG CAACCCAGAG AAGAAGGGTG GTGAAGACTC CCGGCTCTCA 
GCTGCCCCCT GCATCAGACC CAGCAGCTCC CCTCCCACTG TGGCTCCCGC ATCTGCCTCC 
CTGCCCCAGC CCATCCTCTC TAACCAAGGT AATCGTGTAT GTATCTTGCT TCTAGTGGAG 
CCACACAGCC CTGCCTGGGC CCCCTGGCTG GGCTGGGGTT GGGGGAGAGG TGCCAGCACC 
TGCTTCCAAC AGGGTCAGAC ACAGGGAGGG CAGTGCCTTC TGCAGGCTGG TCCTCGCGGG 
GGGACACATG GCAGGGGTGC CTGGCCTGAT GCCAGCTGTT GCTTGCTTGG TGAGGACTCC 
CAATTGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NISCH ( NM_001276294.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001263223.1 Homo sapiens nischarin (NISCH), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu14575
Clone ID Related Accession (Same CDS sequence)	NM_001276294.1
Accession Version	NM_001276294.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	nischarin isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006208.3, AL703940.1, BC050552.1, BC038102.2 and BU177208.1. Transcript Variant: This variant (3) lacks several exons and includes an alternate 3' terminal exon, resulting in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 3 (also known as IRAS-S) which is shorter and has a distinct C-terminus, compared to isoform 1. This isoform lacks multiple domains which are believed to be required by functional imidazoline receptors. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.19215.1, SRR1803617.212540.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCGACCG CGCGCACCTT CGGGCCCGAG CGGGAAGCCG AGCCGGCCAA GGAAGCGCGC 
GTCGTGGGCT CGGAGCTTGT GGACACTTAT ACGGTTTACA TCATCCAGGT CACTGATGGC 
AGCCATGAGT GGACAGTAAA GCACCGCTAC AGCGACTTCC ATGACCTGCA TGAAAAGCTC 
GTTGCAGAGA GAAAGATTGA TAAAAATCTG CTTCCGCCCA AAAAGATAAT TGGGAAAAAC 
TCAAGAAGCT TGGTGGAGAA GAGGGAGAAG GATCTGGAGG TCTACCTCCA GAAGCTCCTG 
GCTGCCTTCC CTGGCGTGAC CCCCAGAGTA CTGGCCCACT TCTTGCATTT TCACTTCTAT 
GAGATAAATG GCATCACCGC GGCACTGGCT GAAGAGCTCT TTGAGAAAGG AGAACAGCTC 
CTGGGGGCCG GCGAGGTCTT TGCCATTGGA CCCCTGCAGC TGTATGCCGT CACGGAGCAG 
CTGCAGCAGG GAAAGCCCAC GTGCGCCAGT GGGGATGCCA AGACCGACCT CGGGCACATC 
CTGGACTTCA CCTGTCGCCT TAAGTACCTT AAGGTTTCTG GCACAGAAGG ACCTTTTGGG 
ACCAGCAACA TTCAGGAGCA GCTCCTGCCG TTCGACCTAT CAATATTCAA GTCCCTGCAT 
CAGGTGGAGA TAAGTCACTG TGATGCTAAG CACATCAGAG GGCTGGTCGC ATCGAAGCCC 
ACCTTAGCCA CGCTGAGTGT CCGCTTCTCA GCAACCTCGA TGAAGGAAGT CCTTGTTCCT 
GAAGCCTCAG AATTTGATGA GTGGGAGCCT GAAGGCACAA CCCTAGAAGG CCCTGTGACT 
GCCGTCATCC CCACTTGGCA GGCATTGACC ACGCTTGACC TGAGCCACAA CAGCATCTCC 
GAGATCGACG AGTCTGTGAA ACTGATCCCA AAGATTGAGT TCCTGGACCT GAGTCACAAT 
GGATTGCTGG TTGTGGACAA TCTGCAGCAC CTGTATAACC TTGTGCATCT GGACCTGTCC 
TACAACAAGC TCTCCTCCTT GGAAGGGCTT CACACCAAGC TGGGGAACAT CAAGACCTTA 
AACCTGGCAG GCAACCTCCT AGAGAGTCTG AGTGGCCTGC ACAAGCTCTA CTCACTGGTC 
AACCTGGATC TCCGGGACAA CAGGATCGAA CAGATGGAGG AGGTCCGGAG CATAGGCAGC 
CTCCCGTGTC TGGAGCACGT GTCTCTGCTG AACAACCCTC TGAGCATCAT CCCCGACTAC 
CGGACCAAGG TGCTGGCTCA GTTCGGAGAG AGGGCCTCAG AGGTCTGTCT GGATGACACA 
GTGACCACAG AGAAGGAGCT GGACACTGTG GAAGTGCTGA AAGCAATTCA GAAAGCCAAG 
GAGGTCAAGT CCAAACTGAG CAACCCAGAG AAGAAGGGTG GTGAAGACTC CCGGCTCTCA 
GCTGCCCCCT GCATCAGACC CAGCAGCTCC CCTCCCACTG TGGCTCCCGC ATCTGCCTCC 
CTGCCCCAGC CCATCCTCTC TAACCAAGGC ATCCTCGGAG ATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263223.1
CDS	135..1682
Translation	MATARTFGPEREAEPAKEARVVGSELVDTYTVYIIQVTDGSHEWTVKHRYSDFHDLHEKLVAERKIDKNLLPPKKIIGKNSRSLVEKREKDLEVYLQKLLAAFPGVTPRVLAHFLHFHFYEINGITAALAEELFEKGEQLLGAGEVFAIGPLQLYAVTEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIQEQLLPFDLSIFKSLHQVEISHCDAKHIRGLVASKPTLATLSVRFSATSMKEVLVPEASEFDEWEPEGTTLEGPVTAVIPTWQALTTLDLSHNSISEIDESVKLIPKIEFLDLSHNGLLVVDNLQHLYNLVHLDLSYNKLSSLEGLHTKLGNIKTLNLAGNLLESLSGLHKLYSLVNLDLRDNRIEQMEEVRSIGSLPCLEHVSLLNNPLSIIPDYRTKVLAQFGERASEVCLDDTVTTEKELDTVEVLKAIQKAKEVKSKLSNPEKKGGEDSRLSAAPCIRPSSSPPTVAPASASLPQPILSNQGILGDE

Target ORF information:

RefSeq Version	NM_001276294.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nischarin (NISCH), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276294.1

ORF Insert Sequence:

ATGGCGACCG CGCGCACCTT CGGGCCCGAG CGGGAAGCCG AGCCGGCCAA GGAAGCGCGC 
GTCGTGGGCT CGGAGCTTGT GGACACTTAT ACGGTTTACA TCATCCAGGT CACTGATGGC 
AGCCATGAGT GGACAGTAAA GCACCGCTAC AGCGACTTCC ATGACCTGCA TGAAAAGCTC 
GTTGCAGAGA GAAAGATTGA TAAAAATCTG CTTCCGCCCA AAAAGATAAT TGGGAAAAAC 
TCAAGAAGCT TGGTGGAGAA GAGGGAGAAG GATCTGGAGG TCTACCTCCA GAAGCTCCTG 
GCTGCCTTCC CTGGCGTGAC CCCCAGAGTA CTGGCCCACT TCTTGCATTT TCACTTCTAT 
GAGATAAATG GCATCACCGC GGCACTGGCT GAAGAGCTCT TTGAGAAAGG AGAACAGCTC 
CTGGGGGCCG GCGAGGTCTT TGCCATTGGA CCCCTGCAGC TGTATGCCGT CACGGAGCAG 
CTGCAGCAGG GAAAGCCCAC GTGCGCCAGT GGGGATGCCA AGACCGACCT CGGGCACATC 
CTGGACTTCA CCTGTCGCCT TAAGTACCTT AAGGTTTCTG GCACAGAAGG ACCTTTTGGG 
ACCAGCAACA TTCAGGAGCA GCTCCTGCCG TTCGACCTAT CAATATTCAA GTCCCTGCAT 
CAGGTGGAGA TAAGTCACTG TGATGCTAAG CACATCAGAG GGCTGGTCGC ATCGAAGCCC 
ACCTTAGCCA CGCTGAGTGT CCGCTTCTCA GCAACCTCGA TGAAGGAAGT CCTTGTTCCT 
GAAGCCTCAG AATTTGATGA GTGGGAGCCT GAAGGCACAA CCCTAGAAGG CCCTGTGACT 
GCCGTCATCC CCACTTGGCA GGCATTGACC ACGCTTGACC TGAGCCACAA CAGCATCTCC 
GAGATCGACG AGTCTGTGAA ACTGATCCCA AAGATTGAGT TCCTGGACCT GAGTCACAAT 
GGATTGCTGG TTGTGGACAA TCTGCAGCAC CTGTATAACC TTGTGCATCT GGACCTGTCC 
TACAACAAGC TCTCCTCCTT GGAAGGGCTT CACACCAAGC TGGGGAACAT CAAGACCTTA 
AACCTGGCAG GCAACCTCCT AGAGAGTCTG AGTGGCCTGC ACAAGCTCTA CTCACTGGTC 
AACCTGGATC TCCGGGACAA CAGGATCGAA CAGATGGAGG AGGTCCGGAG CATAGGCAGC 
CTCCCGTGTC TGGAGCACGT GTCTCTGCTG AACAACCCTC TGAGCATCAT CCCCGACTAC 
CGGACCAAGG TGCTGGCTCA GTTCGGAGAG AGGGCCTCAG AGGTCTGTCT GGATGACACA 
GTGACCACAG AGAAGGAGCT GGACACTGTG GAAGTGCTGA AAGCAATTCA GAAAGCCAAG 
GAGGTCAAGT CCAAACTGAG CAACCCAGAG AAGAAGGGTG GTGAAGACTC CCGGCTCTCA 
GCTGCCCCCT GCATCAGACC CAGCAGCTCC CCTCCCACTG TGGCTCCCGC ATCTGCCTCC 
CTGCCCCAGC CCATCCTCTC TAACCAAGGC ATCCTCGGAG ATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NISCH ( NM_001276294.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001263223.2 Homo sapiens nischarin (NISCH), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu108698
Clone ID Related Accession (Same CDS sequence)	NM_001276294.2
Accession Version	NM_001276294.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	nischarin isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006208.3. On May 31, 2019 this sequence version replaced NM_001276294.1. Transcript Variant: This variant (3) lacks several exons and includes an alternate 3' terminal exon, resulting in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 3 (also known as IRAS-S) which is shorter and has a distinct C-terminus, compared to isoform 1. This isoform lacks multiple domains which are believed to be required by functional imidazoline receptors. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.83944.1, SRR1803615.81422.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCGACCG CGCGCACCTT CGGGCCCGAG CGGGAAGCCG AGCCGGCCAA GGAAGCGCGC 
GTCGTGGGCT CGGAGCTTGT GGACACTTAT ACGGTTTACA TCATCCAGGT CACTGATGGC 
AGCCATGAGT GGACAGTAAA GCACCGCTAC AGCGACTTCC ATGACCTGCA TGAAAAGCTC 
GTTGCAGAGA GAAAGATTGA TAAAAATCTG CTTCCGCCCA AAAAGATAAT TGGGAAAAAC 
TCAAGAAGCT TGGTGGAGAA GAGGGAGAAG GATCTGGAGG TCTACCTCCA GAAGCTCCTG 
GCTGCCTTCC CTGGCGTGAC CCCCAGAGTA CTGGCCCACT TCTTGCATTT TCACTTCTAT 
GAGATAAATG GCATCACCGC GGCACTGGCT GAAGAGCTCT TTGAGAAAGG AGAACAGCTC 
CTGGGGGCCG GCGAGGTCTT TGCCATTGGA CCCCTGCAGC TGTATGCCGT CACGGAGCAG 
CTGCAGCAGG GAAAGCCCAC GTGCGCCAGT GGGGATGCCA AGACCGACCT CGGGCACATC 
CTGGACTTCA CCTGTCGCCT TAAGTACCTT AAGGTTTCTG GCACAGAAGG ACCTTTTGGG 
ACCAGCAACA TTCAGGAGCA GCTCCTGCCG TTCGACCTAT CAATATTCAA GTCCCTGCAT 
CAGGTGGAGA TAAGTCACTG TGATGCTAAG CACATCAGAG GGCTGGTCGC ATCGAAGCCC 
ACCTTAGCCA CGCTGAGTGT CCGCTTCTCA GCAACCTCGA TGAAGGAAGT CCTTGTTCCT 
GAAGCCTCAG AATTTGATGA GTGGGAGCCT GAAGGCACAA CCCTAGAAGG CCCTGTGACT 
GCCGTCATCC CCACTTGGCA GGCATTGACC ACGCTTGACC TGAGCCACAA CAGCGTCTCC 
GAGATCGACG AGTCTGTGAA ACTGATCCCA AAGATTGAGT TCCTGGACCT GAGTCACAAT 
GGATTGCTGG TTGTGGACAA TCTGCAGCAC CTGTATAACC TTGTGCATCT GGACCTGTCC 
TACAACAAGC TCTCCTCCTT GGAAGGGCTT CACACCAAGC TGGGGAACAT CAAGACCTTA 
AACCTGGCAG GCAACCTCCT AGAGAGTCTG AGTGGCCTGC ACAAGCTCTA CTCACTGGTC 
AACCTGGATC TCCGGGACAA CAGGATCGAA CAGATGGAGG AGGTCCGGAG CATAGGCAGC 
CTCCCGTGTC TGGAGCACGT GTCTCTGCTG AACAACCCTC TGAGCATCAT CCCCGACTAC 
CGGACCAAGG TGCTGGCTCA GTTCGGAGAG AGGGCCTCAG AGGTCTGTCT GGATGACACA 
GTGACCACAG AGAAGGAGCT GGACACTGTG GAAGTGCTGA AAGCAATTCA GAAAGCCAAG 
GAGGTCAAGT CCAAACTGAG CAACCCAGAG AAGAAGGGTG GTGAAGACTC CCGGCTCTCA 
GCTGCCCCCT GCATCAGACC CAGCAGCTCC CCTCCCACTG TGGCTCCCGC ATCTGCCTCC 
CTGCCCCAGC CCATCCTCTC TAACCAAGGC ATCCTCGGAG ATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263223.2
CDS	39..1586
Translation	MATARTFGPEREAEPAKEARVVGSELVDTYTVYIIQVTDGSHEWTVKHRYSDFHDLHEKLVAERKIDKNLLPPKKIIGKNSRSLVEKREKDLEVYLQKLLAAFPGVTPRVLAHFLHFHFYEINGITAALAEELFEKGEQLLGAGEVFAIGPLQLYAVTEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIQEQLLPFDLSIFKSLHQVEISHCDAKHIRGLVASKPTLATLSVRFSATSMKEVLVPEASEFDEWEPEGTTLEGPVTAVIPTWQALTTLDLSHNSVSEIDESVKLIPKIEFLDLSHNGLLVVDNLQHLYNLVHLDLSYNKLSSLEGLHTKLGNIKTLNLAGNLLESLSGLHKLYSLVNLDLRDNRIEQMEEVRSIGSLPCLEHVSLLNNPLSIIPDYRTKVLAQFGERASEVCLDDTVTTEKELDTVEVLKAIQKAKEVKSKLSNPEKKGGEDSRLSAAPCIRPSSSPPTVAPASASLPQPILSNQGILGDE

Target ORF information:

RefSeq Version	NM_001276294.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nischarin (NISCH), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276294.2

ORF Insert Sequence:

ATGGCGACCG CGCGCACCTT CGGGCCCGAG CGGGAAGCCG AGCCGGCCAA GGAAGCGCGC 
GTCGTGGGCT CGGAGCTTGT GGACACTTAT ACGGTTTACA TCATCCAGGT CACTGATGGC 
AGCCATGAGT GGACAGTAAA GCACCGCTAC AGCGACTTCC ATGACCTGCA TGAAAAGCTC 
GTTGCAGAGA GAAAGATTGA TAAAAATCTG CTTCCGCCCA AAAAGATAAT TGGGAAAAAC 
TCAAGAAGCT TGGTGGAGAA GAGGGAGAAG GATCTGGAGG TCTACCTCCA GAAGCTCCTG 
GCTGCCTTCC CTGGCGTGAC CCCCAGAGTA CTGGCCCACT TCTTGCATTT TCACTTCTAT 
GAGATAAATG GCATCACCGC GGCACTGGCT GAAGAGCTCT TTGAGAAAGG AGAACAGCTC 
CTGGGGGCCG GCGAGGTCTT TGCCATTGGA CCCCTGCAGC TGTATGCCGT CACGGAGCAG 
CTGCAGCAGG GAAAGCCCAC GTGCGCCAGT GGGGATGCCA AGACCGACCT CGGGCACATC 
CTGGACTTCA CCTGTCGCCT TAAGTACCTT AAGGTTTCTG GCACAGAAGG ACCTTTTGGG 
ACCAGCAACA TTCAGGAGCA GCTCCTGCCG TTCGACCTAT CAATATTCAA GTCCCTGCAT 
CAGGTGGAGA TAAGTCACTG TGATGCTAAG CACATCAGAG GGCTGGTCGC ATCGAAGCCC 
ACCTTAGCCA CGCTGAGTGT CCGCTTCTCA GCAACCTCGA TGAAGGAAGT CCTTGTTCCT 
GAAGCCTCAG AATTTGATGA GTGGGAGCCT GAAGGCACAA CCCTAGAAGG CCCTGTGACT 
GCCGTCATCC CCACTTGGCA GGCATTGACC ACGCTTGACC TGAGCCACAA CAGCGTCTCC 
GAGATCGACG AGTCTGTGAA ACTGATCCCA AAGATTGAGT TCCTGGACCT GAGTCACAAT 
GGATTGCTGG TTGTGGACAA TCTGCAGCAC CTGTATAACC TTGTGCATCT GGACCTGTCC 
TACAACAAGC TCTCCTCCTT GGAAGGGCTT CACACCAAGC TGGGGAACAT CAAGACCTTA 
AACCTGGCAG GCAACCTCCT AGAGAGTCTG AGTGGCCTGC ACAAGCTCTA CTCACTGGTC 
AACCTGGATC TCCGGGACAA CAGGATCGAA CAGATGGAGG AGGTCCGGAG CATAGGCAGC 
CTCCCGTGTC TGGAGCACGT GTCTCTGCTG AACAACCCTC TGAGCATCAT CCCCGACTAC 
CGGACCAAGG TGCTGGCTCA GTTCGGAGAG AGGGCCTCAG AGGTCTGTCT GGATGACACA 
GTGACCACAG AGAAGGAGCT GGACACTGTG GAAGTGCTGA AAGCAATTCA GAAAGCCAAG 
GAGGTCAAGT CCAAACTGAG CAACCCAGAG AAGAAGGGTG GTGAAGACTC CCGGCTCTCA 
GCTGCCCCCT GCATCAGACC CAGCAGCTCC CCTCCCACTG TGGCTCCCGC ATCTGCCTCC 
CTGCCCCAGC CCATCCTCTC TAACCAAGGC ATCCTCGGAG ATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NISCH ( NM_001276293.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001263222.2 Homo sapiens nischarin (NISCH), transcript variant 2, mRNA.

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CloneID	OHu108703
Clone ID Related Accession (Same CDS sequence)	NM_001276293.2
Accession Version	NM_001276293.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	nischarin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006208.3. On May 31, 2019 this sequence version replaced NM_001276293.1. Transcript Variant: This variant (2) has its transcription extend past a splice site that is used in variant 1, resulting in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2 (also known as IRAS-L) which is shorter and has a distinct C-terminus, compared to isoform 1. This isoform lacks multiple domains which are believed to be required by functional imidazoline receptors. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050552.1, SRR1660805.61416.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCGACCG CGCGCACCTT CGGGCCCGAG CGGGAAGCCG AGCCGGCCAA GGAAGCGCGC 
GTCGTGGGCT CGGAGCTTGT GGACACTTAT ACGGTTTACA TCATCCAGGT CACTGATGGC 
AGCCATGAGT GGACAGTAAA GCACCGCTAC AGCGACTTCC ATGACCTGCA TGAAAAGCTC 
GTTGCAGAGA GAAAGATTGA TAAAAATCTG CTTCCGCCCA AAAAGATAAT TGGGAAAAAC 
TCAAGAAGCT TGGTGGAGAA GAGGGAGAAG GATCTGGAGG TCTACCTCCA GAAGCTCCTG 
GCTGCCTTCC CTGGCGTGAC CCCCAGAGTA CTGGCCCACT TCTTGCATTT TCACTTCTAT 
GAGATAAATG GCATCACCGC GGCACTGGCT GAAGAGCTCT TTGAGAAAGG AGAACAGCTC 
CTGGGGGCCG GCGAGGTCTT TGCCATTGGA CCCCTGCAGC TGTATGCCGT CACGGAGCAG 
CTGCAGCAGG GAAAGCCCAC GTGCGCCAGT GGGGATGCCA AGACCGACCT CGGGCACATC 
CTGGACTTCA CCTGTCGCCT TAAGTACCTT AAGGTTTCTG GCACAGAAGG ACCTTTTGGG 
ACCAGCAACA TTCAGGAGCA GCTCCTGCCG TTCGACCTAT CAATATTCAA GTCCCTGCAT 
CAGGTGGAGA TAAGTCACTG TGATGCTAAG CACATCAGAG GGCTGGTCGC ATCGAAGCCC 
ACCTTAGCCA CGCTGAGTGT CCGCTTCTCA GCAACCTCGA TGAAGGAAGT CCTTGTTCCT 
GAAGCCTCAG AATTTGATGA GTGGGAGCCT GAAGGCACAA CCCTAGAAGG CCCTGTGACT 
GCCGTCATCC CCACTTGGCA GGCATTGACC ACGCTTGACC TGAGCCACAA CAGCGTCTCC 
GAGATCGACG AGTCTGTGAA ACTGATCCCA AAGATTGAGT TCCTGGACCT GAGTCACAAT 
GGATTGCTGG TTGTGGACAA TCTGCAGCAC CTGTATAACC TTGTGCATCT GGACCTGTCC 
TACAACAAGC TCTCCTCCTT GGAAGGGCTT CACACCAAGC TGGGGAACAT CAAGACCTTA 
AACCTGGCAG GCAACCTCCT AGAGAGTCTG AGTGGCCTGC ACAAGCTCTA CTCACTGGTC 
AACCTGGATC TCCGGGACAA CAGGATCGAA CAGATGGAGG AGGTCCGGAG CATAGGCAGC 
CTCCCGTGTC TGGAGCACGT GTCTCTGCTG AACAACCCTC TGAGCATCAT CCCCGACTAC 
CGGACCAAGG TGCTGGCTCA GTTCGGAGAG AGGGCCTCAG AGGTCTGTCT GGATGACACA 
GTGACCACAG AGAAGGAGCT GGACACTGTG GAAGTGCTGA AAGCAATTCA GAAAGCCAAG 
GAGGTCAAGT CCAAACTGAG CAACCCAGAG AAGAAGGGTG GTGAAGACTC CCGGCTCTCA 
GCTGCCCCCT GCATCAGACC CAGCAGCTCC CCTCCCACTG TGGCTCCCGC ATCTGCCTCC 
CTGCCCCAGC CCATCCTCTC TAACCAAGGT AATCGTGTAT GTATCTTGCT TCTAGTGGAG 
CCACACAGCC CTGCCTGGGC CCCCTGGCTG GGCTGGGGTT GGGGGAGAGG TGCCAGCACC 
TGCTTCCAAC AGGGTCAGAC ACAGGGAGGG CAGTGCCTTC TGCAGGCTGG TCCTCGCGGG 
GGGACACATG GCAGGGGTGC CTGGCCTGAT GCCAGCTGTT GCTTGCTTGG TGAGGACTCC 
CAATTGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263222.2
CDS	39..1790
Translation	MATARTFGPEREAEPAKEARVVGSELVDTYTVYIIQVTDGSHEWTVKHRYSDFHDLHEKLVAERKIDKNLLPPKKIIGKNSRSLVEKREKDLEVYLQKLLAAFPGVTPRVLAHFLHFHFYEINGITAALAEELFEKGEQLLGAGEVFAIGPLQLYAVTEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIQEQLLPFDLSIFKSLHQVEISHCDAKHIRGLVASKPTLATLSVRFSATSMKEVLVPEASEFDEWEPEGTTLEGPVTAVIPTWQALTTLDLSHNSVSEIDESVKLIPKIEFLDLSHNGLLVVDNLQHLYNLVHLDLSYNKLSSLEGLHTKLGNIKTLNLAGNLLESLSGLHKLYSLVNLDLRDNRIEQMEEVRSIGSLPCLEHVSLLNNPLSIIPDYRTKVLAQFGERASEVCLDDTVTTEKELDTVEVLKAIQKAKEVKSKLSNPEKKGGEDSRLSAAPCIRPSSSPPTVAPASASLPQPILSNQGNRVCILLLVEPHSPAWAPWLGWGWGRGASTCFQQGQTQGGQCLLQAGPRGGTHGRGAWPDASCCLLGEDSQLL

Target ORF information:

RefSeq Version	NM_001276293.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nischarin (NISCH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276293.2

ORF Insert Sequence:

ATGGCGACCG CGCGCACCTT CGGGCCCGAG CGGGAAGCCG AGCCGGCCAA GGAAGCGCGC 
GTCGTGGGCT CGGAGCTTGT GGACACTTAT ACGGTTTACA TCATCCAGGT CACTGATGGC 
AGCCATGAGT GGACAGTAAA GCACCGCTAC AGCGACTTCC ATGACCTGCA TGAAAAGCTC 
GTTGCAGAGA GAAAGATTGA TAAAAATCTG CTTCCGCCCA AAAAGATAAT TGGGAAAAAC 
TCAAGAAGCT TGGTGGAGAA GAGGGAGAAG GATCTGGAGG TCTACCTCCA GAAGCTCCTG 
GCTGCCTTCC CTGGCGTGAC CCCCAGAGTA CTGGCCCACT TCTTGCATTT TCACTTCTAT 
GAGATAAATG GCATCACCGC GGCACTGGCT GAAGAGCTCT TTGAGAAAGG AGAACAGCTC 
CTGGGGGCCG GCGAGGTCTT TGCCATTGGA CCCCTGCAGC TGTATGCCGT CACGGAGCAG 
CTGCAGCAGG GAAAGCCCAC GTGCGCCAGT GGGGATGCCA AGACCGACCT CGGGCACATC 
CTGGACTTCA CCTGTCGCCT TAAGTACCTT AAGGTTTCTG GCACAGAAGG ACCTTTTGGG 
ACCAGCAACA TTCAGGAGCA GCTCCTGCCG TTCGACCTAT CAATATTCAA GTCCCTGCAT 
CAGGTGGAGA TAAGTCACTG TGATGCTAAG CACATCAGAG GGCTGGTCGC ATCGAAGCCC 
ACCTTAGCCA CGCTGAGTGT CCGCTTCTCA GCAACCTCGA TGAAGGAAGT CCTTGTTCCT 
GAAGCCTCAG AATTTGATGA GTGGGAGCCT GAAGGCACAA CCCTAGAAGG CCCTGTGACT 
GCCGTCATCC CCACTTGGCA GGCATTGACC ACGCTTGACC TGAGCCACAA CAGCGTCTCC 
GAGATCGACG AGTCTGTGAA ACTGATCCCA AAGATTGAGT TCCTGGACCT GAGTCACAAT 
GGATTGCTGG TTGTGGACAA TCTGCAGCAC CTGTATAACC TTGTGCATCT GGACCTGTCC 
TACAACAAGC TCTCCTCCTT GGAAGGGCTT CACACCAAGC TGGGGAACAT CAAGACCTTA 
AACCTGGCAG GCAACCTCCT AGAGAGTCTG AGTGGCCTGC ACAAGCTCTA CTCACTGGTC 
AACCTGGATC TCCGGGACAA CAGGATCGAA CAGATGGAGG AGGTCCGGAG CATAGGCAGC 
CTCCCGTGTC TGGAGCACGT GTCTCTGCTG AACAACCCTC TGAGCATCAT CCCCGACTAC 
CGGACCAAGG TGCTGGCTCA GTTCGGAGAG AGGGCCTCAG AGGTCTGTCT GGATGACACA 
GTGACCACAG AGAAGGAGCT GGACACTGTG GAAGTGCTGA AAGCAATTCA GAAAGCCAAG 
GAGGTCAAGT CCAAACTGAG CAACCCAGAG AAGAAGGGTG GTGAAGACTC CCGGCTCTCA 
GCTGCCCCCT GCATCAGACC CAGCAGCTCC CCTCCCACTG TGGCTCCCGC ATCTGCCTCC 
CTGCCCCAGC CCATCCTCTC TAACCAAGGT AATCGTGTAT GTATCTTGCT TCTAGTGGAG 
CCACACAGCC CTGCCTGGGC CCCCTGGCTG GGCTGGGGTT GGGGGAGAGG TGCCAGCACC 
TGCTTCCAAC AGGGTCAGAC ACAGGGAGGG CAGTGCCTTC TGCAGGCTGG TCCTCGCGGG 
GGGACACATG GCAGGGGTGC CTGGCCTGAT GCCAGCTGTT GCTTGCTTGG TGAGGACTCC 
CAATTGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NISCH ( NM_007184.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_009115.3 Homo sapiens nischarin (NISCH), transcript variant 1, mRNA.

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CloneID	OHu107681
Clone ID Related Accession (Same CDS sequence)	NM_007184.4
Accession Version	NM_007184.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	nischarin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006208.3. On Nov 23, 2018 this sequence version replaced NM_007184.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1; also known as IRAS-M). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC038102.2, AF082516.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000345716.9/ ENSP00000339958.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGACCG CGCGCACCTT CGGGCCCGAG CGGGAAGCCG AGCCGGCCAA GGAAGCGCGC 
GTCGTGGGCT CGGAGCTTGT GGACACTTAT ACGGTTTACA TCATCCAGGT CACTGATGGC 
AGCCATGAGT GGACAGTAAA GCACCGCTAC AGCGACTTCC ATGACCTGCA TGAAAAGCTC 
GTTGCAGAGA GAAAGATTGA TAAAAATCTG CTTCCGCCCA AAAAGATAAT TGGGAAAAAC 
TCAAGAAGCT TGGTGGAGAA GAGGGAGAAG GATCTGGAGG TCTACCTCCA GAAGCTCCTG 
GCTGCCTTCC CTGGCGTGAC CCCCAGAGTA CTGGCCCACT TCTTGCATTT TCACTTCTAT 
GAGATAAATG GCATCACCGC GGCACTGGCT GAAGAGCTCT TTGAGAAAGG AGAACAGCTC 
CTGGGGGCCG GCGAGGTCTT TGCCATTGGA CCCCTGCAGC TGTATGCCGT CACGGAGCAG 
CTGCAGCAGG GAAAGCCCAC GTGCGCCAGT GGGGATGCCA AGACCGACCT CGGGCACATC 
CTGGACTTCA CCTGTCGCCT TAAGTACCTT AAGGTTTCTG GCACAGAAGG ACCTTTTGGG 
ACCAGCAACA TTCAGGAGCA GCTCCTGCCG TTCGACCTAT CAATATTCAA GTCCCTGCAT 
CAGGTGGAGA TAAGTCACTG TGATGCTAAG CACATCAGAG GGCTGGTCGC ATCGAAGCCC 
ACCTTAGCCA CGCTGAGTGT CCGCTTCTCA GCAACCTCGA TGAAGGAAGT CCTTGTTCCT 
GAAGCCTCAG AATTTGATGA GTGGGAGCCT GAAGGCACAA CCCTAGAAGG CCCTGTGACT 
GCCGTCATCC CCACTTGGCA GGCATTGACC ACGCTTGACC TGAGCCACAA CAGCGTCTCC 
GAGATCGACG AGTCTGTGAA ACTGATCCCA AAGATTGAGT TCCTGGACCT GAGTCACAAT 
GGATTGCTGG TTGTGGACAA TCTGCAGCAC CTGTATAACC TTGTGCATCT GGACCTGTCC 
TACAACAAGC TCTCCTCCTT GGAAGGGCTT CACACCAAGC TGGGGAACAT CAAGACCTTA 
AACCTGGCAG GCAACCTCCT AGAGAGTCTG AGTGGCCTGC ACAAGCTCTA CTCACTGGTC 
AACCTGGATC TCCGGGACAA CAGGATCGAA CAGATGGAGG AGGTCCGGAG CATAGGCAGC 
CTCCCGTGTC TGGAGCACGT GTCTCTGCTG AACAACCCTC TGAGCATCAT CCCCGACTAC 
CGGACCAAGG TGCTGGCTCA GTTCGGAGAG AGGGCCTCAG AGGTCTGTCT GGATGACACA 
GTGACCACAG AGAAGGAGCT GGACACTGTG GAAGTGCTGA AAGCAATTCA GAAAGCCAAG 
GAGGTCAAGT CCAAACTGAG CAACCCAGAG AAGAAGGGTG GTGAAGACTC CCGGCTCTCA 
GCTGCCCCCT GCATCAGACC CAGCAGCTCC CCTCCCACTG TGGCTCCCGC ATCTGCCTCC 
CTGCCCCAGC CCATCCTCTC TAACCAAGGA ATCATGTTCG TTCAGGAGGA GGCCCTGGCC 
AGCAGCCTCT CGTCCACTGA CAGTCTGACT CCCGAGCACC AGCCCATTGC CCAGGGATGT 
TCTGATTCCT TGGAGTCCAT CCCTGCGGGA CAGGCAGCTT CCGATGATTT AAGGGACGTG 
CCAGGAGCTG TTGGTGGTGC AAGCCCAGAA CATGCCGAGC CGGAGGTCCA GGTGGTGCCG 
GGGTCTGGCC AGATCATCTT CCTGCCCTTC ACCTGCATTG GCTACACGGC CACCAATCAG 
GACTTCATCC AGCGCCTGAG CACACTGATC CGGCAGGCCA TCGAGCGGCA GCTGCCTGCC 
TGGATCGAGG CTGCCAACCA GCGGGAGGAG GGCCAGGGTG AACAGGGCGA GGAGGAGGAT 
GAGGAGGAGG AAGAAGAGGA GGACGTGGCT GAGAACCGCT ACTTTGAAAT GGGGCCCCCA 
GACGTGGAGG AGGAGGAGGG AGGAGGCCAG GGGGAGGAAG AGGAGGAGGA AGAGGAGGAT 
GAAGAGGCCG AGGAGGAGCG CCTGGCTCTG GAATGGGCCC TGGGCGCGGA CGAGGACTTC 
CTGCTGGAGC ACATCCGCAT CCTCAAGGTG CTGTGGTGCT TCCTGATCCA TGTGCAGGGC 
AGTATCCGCC AGTTCGCCGC CTGCCTTGTG CTCACCGACT TCGGCATCGC AGTCTTCGAG 
ATCCCGCACC AGGAGTCTCG GGGCAGCAGC CAGCACATCC TCTCCTCCCT GCGCTTTGTC 
TTTTGCTTCC CGCATGGCGA CCTCACCGAG TTTGGCTTCC TCATGCCGGA GCTGTGTCTG 
GTGCTCAAGG TACGGCACAG TGAGAACACG CTCTTCATTA TCTCGGACGC CGCCAACCTG 
CACGAGTTCC ACGCGGACCT GCGCTCATGC TTTGCACCCC AGCACATGGC CATGCTGTGT 
AGCCCCATCC TCTACGGCAG CCACACCAGC CTGCAGGAGT TCCTGCGCCA GCTGCTCACC 
TTCTACAAGG TGGCTGGCGG CTGCCAGGAG CGCAGCCAGG GCTGCTTCCC CGTCTACCTG 
GTCTACAGTG ACAAGCGCAT GGTGCAGACG GCCGCCGGGG ACTACTCAGG CAACATCGAG 
TGGGCCAGCT GCACACTCTG TTCAGCCGTG CGGCGCTCCT GCTGCGCGCC CTCTGAGGCC 
GTCAAGTCCG CCGCCATCCC CTACTGGCTG TTGCTCACGC CCCAGCACCT CAACGTCATC 
AAGGCCGACT TCAACCCCAT GCCCAACCGT GGCACCCACA ACTGTCGCAA CCGCAACAGC 
TTCAAGCTCA GCCGTGTGCC GCTCTCCACC GTGCTGCTGG ACCCCACACG CAGCTGTACC 
CAGCCTCGGG GCGCCTTTGC TGATGGCCAC GTGCTAGAGC TGCTCGTGGG GTACCGCTTT 
GTCACTGCCA TCTTCGTGCT GCCCCACGAG AAGTTCCACT TCCTGCGCGT CTACAACCAG 
CTGCGGGCCT CGCTGCAGGA CCTGAAGACT GTGGTCATCG CCAAGACCCC CGGGACGGGA 
GGCAGCCCCC AGGGCTCCTT TGCGGATGGC CAGCCTGCCG AGCGCAGGGC CAGCAATGAC 
CAGCGTCCCC AGGAGGTCCC AGCAGAGGCT CTGGCCCCGG CCCCAGCGGA AGTCCCAGCT 
CCAGCCCCTG CAGCAGCCTC AGCCTCAGGC CCAGCGAAGA CTCCGGCCCC AGCAGAGGCC 
TCAACTTCAG CTTTGGTCCC AGAGGAGACG CCAGTGGAAG CTCCAGCCCC ACCCCCAGCC 
GAGGCCCCTG CCCAGTACCC GAGTGAGCAC CTCATCCAGG CCACCTCGGA GGAGAATCAG 
ATCCCCTCGC ACTTGCCTGC CTGCCCGTCG CTCCGGCACG TCGCCAGCCT GCGGGGCAGC 
GCCATCATCG AGCTCTTCCA CAGCAGCATT GCTGAGGTTG AAAACGAGGA GCTGAGGCAC 
CTCATGTGGT CCTCGGTGGT GTTCTACCAG ACCCCAGGGC TGGAGGTGAC TGCCTGCGTG 
CTGCTCTCCA CCAAGGCTGT GTACTTTGTG CTCCACGACG GCCTCCGCCG CTACTTCTCA 
GAGCCACTGC AGGATTTCTG GCATCAGAAA AACACCGACT ACAACAACAG CCCTTTCCAC 
ATCTCCCAGT GCTTCGTGCT AAAGCTTAGT GACCTGCAGT CAGTCAATGT GGGGCTTTTC 
GACCAGCATT TCCGGCTGAC GGGTTCCACC CCGATGCAGG TGGTCACGTG CTTGACGCGG 
GACAGCTACC TGACGCACTG CTTCCTCCAG CACCTCATGG TCGTGCTGTC CTCTCTGGAA 
CGCACGCCCT CGCCGGAGCC TGTTGACAAG GACTTCTACT CCGAGTTTGG GAACAAGACC 
ACAGGGAAGA TGGAGAACTA CGAGCTGATC CACTCTAGTC GCGTCAAGTT TACCTACCCC 
AGTGAGGAGG AGATTGGGGA CCTGACGTTC ACTGTGGCCC AAAAGATGGC TGAGCCAGAG 
AAGGCCCCAG CCCTCAGCAT CCTGCTGTAC GTGCAGGCCT TCCAGGTGGG CATGCCACCC 
CCTGGGTGCT GCAGGGGCCC CCTGCGCCCC AAGACACTCC TGCTCACCAG CTCCGAGATC 
TTCCTCCTGG ATGAGGACTG TGTCCACTAC CCACTGCCCG AGTTTGCCAA AGAGCCGCCG 
CAGAGAGACA GGTACCGGCT GGACGATGGC CGCCGCGTCC GGGACCTGGA CCGAGTGCTC 
ATGGGCTACC AGACCTACCC GCAGGCCCTC ACCCTCGTCT TCGATGACGT GCAAGGTCAT 
GACCTCATGG GCAGTGTCAC CCTGGACCAC TTTGGGGAGG TGCCAGGTGG CCCGGCTAGA 
GCCAGCCAGG GCCGTGAAGT CCAGTGGCAG GTGTTTGTCC CCAGTGCTGA GAGCAGAGAG 
AAGCTCATCT CGCTGTTGGC TCGCCAGTGG GAGGCCCTGT GTGGCCGTGA GCTGCCTGTC 
GAGCTCACCG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009115.3
CDS	39..4553
Translation	MATARTFGPEREAEPAKEARVVGSELVDTYTVYIIQVTDGSHEWTVKHRYSDFHDLHEKLVAERKIDKNLLPPKKIIGKNSRSLVEKREKDLEVYLQKLLAAFPGVTPRVLAHFLHFHFYEINGITAALAEELFEKGEQLLGAGEVFAIGPLQLYAVTEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIQEQLLPFDLSIFKSLHQVEISHCDAKHIRGLVASKPTLATLSVRFSATSMKEVLVPEASEFDEWEPEGTTLEGPVTAVIPTWQALTTLDLSHNSVSEIDESVKLIPKIEFLDLSHNGLLVVDNLQHLYNLVHLDLSYNKLSSLEGLHTKLGNIKTLNLAGNLLESLSGLHKLYSLVNLDLRDNRIEQMEEVRSIGSLPCLEHVSLLNNPLSIIPDYRTKVLAQFGERASEVCLDDTVTTEKELDTVEVLKAIQKAKEVKSKLSNPEKKGGEDSRLSAAPCIRPSSSPPTVAPASASLPQPILSNQGIMFVQEEALASSLSSTDSLTPEHQPIAQGCSDSLESIPAGQAASDDLRDVPGAVGGASPEHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREEGQGEQGEEEDEEEEEEEDVAENRYFEMGPPDVEEEEGGGQGEEEEEEEEDEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTSLQEFLRQLLTFYKVAGGCQERSQGCFPVYLVYSDKRMVQTAAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTPQHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVIAKTPGTGGSPQGSFADGQPAERRASNDQRPQEVPAEALAPAPAEVPAPAPAAASASGPAKTPAPAEASTSALVPEETPVEAPAPPPAEAPAQYPSEHLIQATSEENQIPSHLPACPSLRHVASLRGSAIIELFHSSIAEVENEELRHLMWSSVVFYQTPGLEVTACVLLSTKAVYFVLHDGLRRYFSEPLQDFWHQKNTDYNNSPFHISQCFVLKLSDLQSVNVGLFDQHFRLTGSTPMQVVTCLTRDSYLTHCFLQHLMVVLSSLERTPSPEPVDKDFYSEFGNKTTGKMENYELIHSSRVKFTYPSEEEIGDLTFTVAQKMAEPEKAPALSILLYVQAFQVGMPPPGCCRGPLRPKTLLLTSSEIFLLDEDCVHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYQTYPQALTLVFDDVQGHDLMGSVTLDHFGEVPGGPARASQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

Target ORF information:

RefSeq Version	NM_007184.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nischarin (NISCH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007184.4

ORF Insert Sequence:

ATGGCGACCG CGCGCACCTT CGGGCCCGAG CGGGAAGCCG AGCCGGCCAA GGAAGCGCGC 
GTCGTGGGCT CGGAGCTTGT GGACACTTAT ACGGTTTACA TCATCCAGGT CACTGATGGC 
AGCCATGAGT GGACAGTAAA GCACCGCTAC AGCGACTTCC ATGACCTGCA TGAAAAGCTC 
GTTGCAGAGA GAAAGATTGA TAAAAATCTG CTTCCGCCCA AAAAGATAAT TGGGAAAAAC 
TCAAGAAGCT TGGTGGAGAA GAGGGAGAAG GATCTGGAGG TCTACCTCCA GAAGCTCCTG 
GCTGCCTTCC CTGGCGTGAC CCCCAGAGTA CTGGCCCACT TCTTGCATTT TCACTTCTAT 
GAGATAAATG GCATCACCGC GGCACTGGCT GAAGAGCTCT TTGAGAAAGG AGAACAGCTC 
CTGGGGGCCG GCGAGGTCTT TGCCATTGGA CCCCTGCAGC TGTATGCCGT CACGGAGCAG 
CTGCAGCAGG GAAAGCCCAC GTGCGCCAGT GGGGATGCCA AGACCGACCT CGGGCACATC 
CTGGACTTCA CCTGTCGCCT TAAGTACCTT AAGGTTTCTG GCACAGAAGG ACCTTTTGGG 
ACCAGCAACA TTCAGGAGCA GCTCCTGCCG TTCGACCTAT CAATATTCAA GTCCCTGCAT 
CAGGTGGAGA TAAGTCACTG TGATGCTAAG CACATCAGAG GGCTGGTCGC ATCGAAGCCC 
ACCTTAGCCA CGCTGAGTGT CCGCTTCTCA GCAACCTCGA TGAAGGAAGT CCTTGTTCCT 
GAAGCCTCAG AATTTGATGA GTGGGAGCCT GAAGGCACAA CCCTAGAAGG CCCTGTGACT 
GCCGTCATCC CCACTTGGCA GGCATTGACC ACGCTTGACC TGAGCCACAA CAGCGTCTCC 
GAGATCGACG AGTCTGTGAA ACTGATCCCA AAGATTGAGT TCCTGGACCT GAGTCACAAT 
GGATTGCTGG TTGTGGACAA TCTGCAGCAC CTGTATAACC TTGTGCATCT GGACCTGTCC 
TACAACAAGC TCTCCTCCTT GGAAGGGCTT CACACCAAGC TGGGGAACAT CAAGACCTTA 
AACCTGGCAG GCAACCTCCT AGAGAGTCTG AGTGGCCTGC ACAAGCTCTA CTCACTGGTC 
AACCTGGATC TCCGGGACAA CAGGATCGAA CAGATGGAGG AGGTCCGGAG CATAGGCAGC 
CTCCCGTGTC TGGAGCACGT GTCTCTGCTG AACAACCCTC TGAGCATCAT CCCCGACTAC 
CGGACCAAGG TGCTGGCTCA GTTCGGAGAG AGGGCCTCAG AGGTCTGTCT GGATGACACA 
GTGACCACAG AGAAGGAGCT GGACACTGTG GAAGTGCTGA AAGCAATTCA GAAAGCCAAG 
GAGGTCAAGT CCAAACTGAG CAACCCAGAG AAGAAGGGTG GTGAAGACTC CCGGCTCTCA 
GCTGCCCCCT GCATCAGACC CAGCAGCTCC CCTCCCACTG TGGCTCCCGC ATCTGCCTCC 
CTGCCCCAGC CCATCCTCTC TAACCAAGGA ATCATGTTCG TTCAGGAGGA GGCCCTGGCC 
AGCAGCCTCT CGTCCACTGA CAGTCTGACT CCCGAGCACC AGCCCATTGC CCAGGGATGT 
TCTGATTCCT TGGAGTCCAT CCCTGCGGGA CAGGCAGCTT CCGATGATTT AAGGGACGTG 
CCAGGAGCTG TTGGTGGTGC AAGCCCAGAA CATGCCGAGC CGGAGGTCCA GGTGGTGCCG 
GGGTCTGGCC AGATCATCTT CCTGCCCTTC ACCTGCATTG GCTACACGGC CACCAATCAG 
GACTTCATCC AGCGCCTGAG CACACTGATC CGGCAGGCCA TCGAGCGGCA GCTGCCTGCC 
TGGATCGAGG CTGCCAACCA GCGGGAGGAG GGCCAGGGTG AACAGGGCGA GGAGGAGGAT 
GAGGAGGAGG AAGAAGAGGA GGACGTGGCT GAGAACCGCT ACTTTGAAAT GGGGCCCCCA 
GACGTGGAGG AGGAGGAGGG AGGAGGCCAG GGGGAGGAAG AGGAGGAGGA AGAGGAGGAT 
GAAGAGGCCG AGGAGGAGCG CCTGGCTCTG GAATGGGCCC TGGGCGCGGA CGAGGACTTC 
CTGCTGGAGC ACATCCGCAT CCTCAAGGTG CTGTGGTGCT TCCTGATCCA TGTGCAGGGC 
AGTATCCGCC AGTTCGCCGC CTGCCTTGTG CTCACCGACT TCGGCATCGC AGTCTTCGAG 
ATCCCGCACC AGGAGTCTCG GGGCAGCAGC CAGCACATCC TCTCCTCCCT GCGCTTTGTC 
TTTTGCTTCC CGCATGGCGA CCTCACCGAG TTTGGCTTCC TCATGCCGGA GCTGTGTCTG 
GTGCTCAAGG TACGGCACAG TGAGAACACG CTCTTCATTA TCTCGGACGC CGCCAACCTG 
CACGAGTTCC ACGCGGACCT GCGCTCATGC TTTGCACCCC AGCACATGGC CATGCTGTGT 
AGCCCCATCC TCTACGGCAG CCACACCAGC CTGCAGGAGT TCCTGCGCCA GCTGCTCACC 
TTCTACAAGG TGGCTGGCGG CTGCCAGGAG CGCAGCCAGG GCTGCTTCCC CGTCTACCTG 
GTCTACAGTG ACAAGCGCAT GGTGCAGACG GCCGCCGGGG ACTACTCAGG CAACATCGAG 
TGGGCCAGCT GCACACTCTG TTCAGCCGTG CGGCGCTCCT GCTGCGCGCC CTCTGAGGCC 
GTCAAGTCCG CCGCCATCCC CTACTGGCTG TTGCTCACGC CCCAGCACCT CAACGTCATC 
AAGGCCGACT TCAACCCCAT GCCCAACCGT GGCACCCACA ACTGTCGCAA CCGCAACAGC 
TTCAAGCTCA GCCGTGTGCC GCTCTCCACC GTGCTGCTGG ACCCCACACG CAGCTGTACC 
CAGCCTCGGG GCGCCTTTGC TGATGGCCAC GTGCTAGAGC TGCTCGTGGG GTACCGCTTT 
GTCACTGCCA TCTTCGTGCT GCCCCACGAG AAGTTCCACT TCCTGCGCGT CTACAACCAG 
CTGCGGGCCT CGCTGCAGGA CCTGAAGACT GTGGTCATCG CCAAGACCCC CGGGACGGGA 
GGCAGCCCCC AGGGCTCCTT TGCGGATGGC CAGCCTGCCG AGCGCAGGGC CAGCAATGAC 
CAGCGTCCCC AGGAGGTCCC AGCAGAGGCT CTGGCCCCGG CCCCAGCGGA AGTCCCAGCT 
CCAGCCCCTG CAGCAGCCTC AGCCTCAGGC CCAGCGAAGA CTCCGGCCCC AGCAGAGGCC 
TCAACTTCAG CTTTGGTCCC AGAGGAGACG CCAGTGGAAG CTCCAGCCCC ACCCCCAGCC 
GAGGCCCCTG CCCAGTACCC GAGTGAGCAC CTCATCCAGG CCACCTCGGA GGAGAATCAG 
ATCCCCTCGC ACTTGCCTGC CTGCCCGTCG CTCCGGCACG TCGCCAGCCT GCGGGGCAGC 
GCCATCATCG AGCTCTTCCA CAGCAGCATT GCTGAGGTTG AAAACGAGGA GCTGAGGCAC 
CTCATGTGGT CCTCGGTGGT GTTCTACCAG ACCCCAGGGC TGGAGGTGAC TGCCTGCGTG 
CTGCTCTCCA CCAAGGCTGT GTACTTTGTG CTCCACGACG GCCTCCGCCG CTACTTCTCA 
GAGCCACTGC AGGATTTCTG GCATCAGAAA AACACCGACT ACAACAACAG CCCTTTCCAC 
ATCTCCCAGT GCTTCGTGCT AAAGCTTAGT GACCTGCAGT CAGTCAATGT GGGGCTTTTC 
GACCAGCATT TCCGGCTGAC GGGTTCCACC CCGATGCAGG TGGTCACGTG CTTGACGCGG 
GACAGCTACC TGACGCACTG CTTCCTCCAG CACCTCATGG TCGTGCTGTC CTCTCTGGAA 
CGCACGCCCT CGCCGGAGCC TGTTGACAAG GACTTCTACT CCGAGTTTGG GAACAAGACC 
ACAGGGAAGA TGGAGAACTA CGAGCTGATC CACTCTAGTC GCGTCAAGTT TACCTACCCC 
AGTGAGGAGG AGATTGGGGA CCTGACGTTC ACTGTGGCCC AAAAGATGGC TGAGCCAGAG 
AAGGCCCCAG CCCTCAGCAT CCTGCTGTAC GTGCAGGCCT TCCAGGTGGG CATGCCACCC 
CCTGGGTGCT GCAGGGGCCC CCTGCGCCCC AAGACACTCC TGCTCACCAG CTCCGAGATC 
TTCCTCCTGG ATGAGGACTG TGTCCACTAC CCACTGCCCG AGTTTGCCAA AGAGCCGCCG 
CAGAGAGACA GGTACCGGCT GGACGATGGC CGCCGCGTCC GGGACCTGGA CCGAGTGCTC 
ATGGGCTACC AGACCTACCC GCAGGCCCTC ACCCTCGTCT TCGATGACGT GCAAGGTCAT 
GACCTCATGG GCAGTGTCAC CCTGGACCAC TTTGGGGAGG TGCCAGGTGG CCCGGCTAGA 
GCCAGCCAGG GCCGTGAAGT CCAGTGGCAG GTGTTTGTCC CCAGTGCTGA GAGCAGAGAG 
AAGCTCATCT CGCTGTTGGC TCGCCAGTGG GAGGCCCTGT GTGGCCGTGA GCTGCCTGTC 
GAGCTCACCG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NISCH ( NM_007184.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_009115.2 Homo sapiens nischarin (NISCH), transcript variant 1, mRNA.

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CloneID	OHu19507
Clone ID Related Accession (Same CDS sequence)	NM_007184.3
Accession Version	NM_007184.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	nischarin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB496030.1, AC006208.3, BC038102.2 and AI889216.1. This sequence is a reference standard in the RefSeqGene project. On Apr 11, 2007 this sequence version replaced NM_007184.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1; also known as IRAS-M). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC054494.1, AF082516.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCGACCG CGCGCACCTT CGGGCCCGAG CGGGAAGCCG AGCCGGCCAA GGAAGCGCGC 
GTCGTGGGCT CGGAGCTTGT GGACACTTAT ACGGTTTACA TCATCCAGGT CACTGATGGC 
AGCCATGAGT GGACAGTAAA GCACCGCTAC AGCGACTTCC ATGACCTGCA TGAAAAGCTC 
GTTGCAGAGA GAAAGATTGA TAAAAATCTG CTTCCGCCCA AAAAGATAAT TGGGAAAAAC 
TCAAGAAGCT TGGTGGAGAA GAGGGAGAAG GATCTGGAGG TCTACCTCCA GAAGCTCCTG 
GCTGCCTTCC CTGGCGTGAC CCCCAGAGTA CTGGCCCACT TCTTGCATTT TCACTTCTAT 
GAGATAAATG GCATCACCGC GGCACTGGCT GAAGAGCTCT TTGAGAAAGG AGAACAGCTC 
CTGGGGGCCG GCGAGGTCTT TGCCATTGGA CCCCTGCAGC TGTATGCCGT CACGGAGCAG 
CTGCAGCAGG GAAAGCCCAC GTGCGCCAGT GGGGATGCCA AGACCGACCT CGGGCACATC 
CTGGACTTCA CCTGTCGCCT TAAGTACCTT AAGGTTTCTG GCACAGAAGG ACCTTTTGGG 
ACCAGCAACA TTCAGGAGCA GCTCCTGCCG TTCGACCTAT CAATATTCAA GTCCCTGCAT 
CAGGTGGAGA TAAGTCACTG TGATGCTAAG CACATCAGAG GGCTGGTCGC ATCGAAGCCC 
ACCTTAGCCA CGCTGAGTGT CCGCTTCTCA GCAACCTCGA TGAAGGAAGT CCTTGTTCCT 
GAAGCCTCAG AATTTGATGA GTGGGAGCCT GAAGGCACAA CCCTAGAAGG CCCTGTGACT 
GCCGTCATCC CCACTTGGCA GGCATTGACC ACGCTTGACC TGAGCCACAA CAGCATCTCC 
GAGATCGACG AGTCTGTGAA ACTGATCCCA AAGATTGAGT TCCTGGACCT GAGTCACAAT 
GGATTGCTGG TTGTGGACAA TCTGCAGCAC CTGTATAACC TTGTGCATCT GGACCTGTCC 
TACAACAAGC TCTCCTCCTT GGAAGGGCTT CACACCAAGC TGGGGAACAT CAAGACCTTA 
AACCTGGCAG GCAACCTCCT AGAGAGTCTG AGTGGCCTGC ACAAGCTCTA CTCACTGGTC 
AACCTGGATC TCCGGGACAA CAGGATCGAA CAGATGGAGG AGGTCCGGAG CATAGGCAGC 
CTCCCGTGTC TGGAGCACGT GTCTCTGCTG AACAACCCTC TGAGCATCAT CCCCGACTAC 
CGGACCAAGG TGCTGGCTCA GTTCGGAGAG AGGGCCTCAG AGGTCTGTCT GGATGACACA 
GTGACCACAG AGAAGGAGCT GGACACTGTG GAAGTGCTGA AAGCAATTCA GAAAGCCAAG 
GAGGTCAAGT CCAAACTGAG CAACCCAGAG AAGAAGGGTG GTGAAGACTC CCGGCTCTCA 
GCTGCCCCCT GCATCAGACC CAGCAGCTCC CCTCCCACTG TGGCTCCCGC ATCTGCCTCC 
CTGCCCCAGC CCATCCTCTC TAACCAAGGA ATCATGTTCG TTCAGGAGGA GGCCCTGGCC 
AGCAGCCTCT CGTCCACTGA CAGTCTGACT CCCGAGCACC AGCCCATTGC CCAGGGATGT 
TCTGATTCCT TGGAGTCCAT CCCTGCGGGA CAGGCAGCTT CCGATGATTT AAGGGACGTG 
CCAGGAGCTG TTGGTGGTGC AAGCCCAGAA CATGCCGAGC CGGAGGTCCA GGTGGTGCCG 
GGGTCTGGCC AGATCATCTT CCTGCCCTTC ACCTGCATTG GCTACACGGC CACCAATCAG 
GACTTCATCC AGCGCCTGAG CACACTGATC CGGCAGGCCA TCGAGCGGCA GCTGCCTGCC 
TGGATCGAGG CTGCCAACCA GCGGGAGGAG GGCCAGGGTG AACAGGGCGA GGAGGAGGAT 
GAGGAGGAGG AAGAAGAGGA GGACGTGGCT GAGAACCGCT ACTTTGAAAT GGGGCCCCCA 
GACGTGGAGG AGGAGGAGGG AGGAGGCCAG GGGGAGGAAG AGGAGGAGGA AGAGGAGGAT 
GAAGAGGCCG AGGAGGAGCG CCTGGCTCTG GAATGGGCCC TGGGCGCGGA CGAGGACTTC 
CTGCTGGAGC ACATCCGCAT CCTCAAGGTG CTGTGGTGCT TCCTGATCCA TGTGCAGGGC 
AGTATCCGCC AGTTCGCCGC CTGCCTTGTG CTCACCGACT TCGGCATCGC AGTCTTCGAG 
ATCCCGCACC AGGAGTCTCG GGGCAGCAGC CAGCACATCC TCTCCTCCCT GCGCTTTGTC 
TTTTGCTTCC CGCATGGCGA CCTCACCGAG TTTGGCTTCC TCATGCCGGA GCTGTGTCTG 
GTGCTCAAGG TACGGCACAG TGAGAACACG CTCTTCATTA TCTCGGACGC CGCCAACCTG 
CACGAGTTCC ACGCGGACCT GCGCTCATGC TTTGCACCCC AGCACATGGC CATGCTGTGT 
AGCCCCATCC TCTACGGCAG CCACACCAGC CTGCAGGAGT TCCTGCGCCA GCTGCTCACC 
TTCTACAAGG TGGCTGGCGG CTGCCAGGAG CGCAGCCAGG GCTGCTTCCC CGTCTACCTG 
GTCTACAGTG ACAAGCGCAT GGTGCAGACG GCCGCCGGGG ACTACTCAGG CAACATCGAG 
TGGGCCAGCT GCACACTCTG TTCAGCCGTG CGGCGCTCCT GCTGCGCGCC CTCTGAGGCC 
GTCAAGTCCG CCGCCATCCC CTACTGGCTG TTGCTCACGC CCCAGCACCT CAACGTCATC 
AAGGCCGACT TCAACCCCAT GCCCAACCGT GGCACCCACA ACTGTCGCAA CCGCAACAGC 
TTCAAGCTCA GCCGTGTGCC GCTCTCCACC GTGCTGCTGG ACCCCACACG CAGCTGTACC 
CAGCCTCGGG GCGCCTTTGC TGATGGCCAC GTGCTAGAGC TGCTCGTGGG GTACCGCTTT 
GTCACTGCCA TCTTCGTGCT GCCCCACGAG AAGTTCCACT TCCTGCGCGT CTACAACCAG 
CTGCGGGCCT CGCTGCAGGA CCTGAAGACT GTGGTCATCG CCAAGACCCC CGGGACGGGA 
GGCAGCCCCC AGGGCTCCTT TGCGGATGGC CAGCCTGCCG AGCGCAGGGC CAGCAATGAC 
CAGCGTCCCC AGGAGGTCCC AGCAGAGGCT CTGGCCCCGG CCCCAGCGGA AGTCCCAGCT 
CCAGCCCCTG CAGCAGCCTC AGCCTCAGGC CCAGCGAAGA CTCCGGCCCC AGCAGAGGCC 
TCAACTTCAG CTTTGGTCCC AGAGGAGACG CCAGTGGAAG CTCCAGCCCC ACCCCCAGCC 
GAGGCCCCTG CCCAGTACCC GAGTGAGCAC CTCATCCAGG CCACCTCGGA GGAGAATCAG 
ATCCCCTCGC ACTTGCCTGC CTGCCCGTCG CTCCGGCACG TCGCCAGCCT GCGGGGCAGC 
GCCATCATCG AGCTCTTCCA CAGCAGCATT GCTGAGGTTG AAAACGAGGA GCTGAGGCAC 
CTCATGTGGT CCTCGGTGGT GTTCTACCAG ACCCCAGGGC TGGAGGTGAC TGCCTGCGTG 
CTGCTCTCCA CCAAGGCTGT GTACTTTGTG CTCCACGACG GCCTCCGCCG CTACTTCTCA 
GAGCCACTGC AGGATTTCTG GCATCAGAAA AACACCGACT ACAACAACAG CCCTTTCCAC 
ATCTCCCAGT GCTTCGTGCT AAAGCTTAGT GACCTGCAGT CAGTCAATGT GGGGCTTTTC 
GACCAGCATT TCCGGCTGAC GGGTTCCACC CCGATGCAGG TGGTCACGTG CTTGACGCGG 
GACAGCTACC TGACGCACTG CTTCCTCCAG CACCTCATGG TCGTGCTGTC CTCTCTGGAA 
CGCACGCCCT CGCCGGAGCC TGTTGACAAG GACTTCTACT CCGAGTTTGG GAACAAGACC 
ACAGGGAAGA TGGAGAACTA CGAGCTGATC CACTCTAGTC GCGTCAAGTT TACCTACCCC 
AGTGAGGAGG AGATTGGGGA CCTGACGTTC ACTGTGGCCC AAAAGATGGC TGAGCCAGAG 
AAGGCCCCAG CCCTCAGCAT CCTGCTGTAC GTGCAGGCCT TCCAGGTGGG CATGCCACCC 
CCTGGGTGCT GCAGGGGCCC CCTGCGCCCC AAGACACTCC TGCTCACCAG CTCCGAGATC 
TTCCTCCTGG ATGAGGACTG TGTCCACTAC CCACTGCCCG AGTTTGCCAA AGAGCCGCCG 
CAGAGAGACA GGTACCGGCT GGACGATGGC CGCCGCGTCC GGGACCTGGA CCGAGTGCTC 
ATGGGCTACC AGACCTACCC GCAGGCCCTC ACCCTCGTCT TCGATGACGT GCAAGGTCAT 
GACCTCATGG GCAGTGTCAC CCTGGACCAC TTTGGGGAGG TGCCAGGTGG CCCGGCTAGA 
GCCAGCCAGG GCCGTGAAGT CCAGTGGCAG GTGTTTGTCC CCAGTGCTGA GAGCAGAGAG 
AAGCTCATCT CGCTGTTGGC TCGCCAGTGG GAGGCCCTGT GTGGCCGTGA GCTGCCTGTC 
GAGCTCACCG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009115.2
CDS	135..4649
Translation	MATARTFGPEREAEPAKEARVVGSELVDTYTVYIIQVTDGSHEWTVKHRYSDFHDLHEKLVAERKIDKNLLPPKKIIGKNSRSLVEKREKDLEVYLQKLLAAFPGVTPRVLAHFLHFHFYEINGITAALAEELFEKGEQLLGAGEVFAIGPLQLYAVTEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIQEQLLPFDLSIFKSLHQVEISHCDAKHIRGLVASKPTLATLSVRFSATSMKEVLVPEASEFDEWEPEGTTLEGPVTAVIPTWQALTTLDLSHNSISEIDESVKLIPKIEFLDLSHNGLLVVDNLQHLYNLVHLDLSYNKLSSLEGLHTKLGNIKTLNLAGNLLESLSGLHKLYSLVNLDLRDNRIEQMEEVRSIGSLPCLEHVSLLNNPLSIIPDYRTKVLAQFGERASEVCLDDTVTTEKELDTVEVLKAIQKAKEVKSKLSNPEKKGGEDSRLSAAPCIRPSSSPPTVAPASASLPQPILSNQGIMFVQEEALASSLSSTDSLTPEHQPIAQGCSDSLESIPAGQAASDDLRDVPGAVGGASPEHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREEGQGEQGEEEDEEEEEEEDVAENRYFEMGPPDVEEEEGGGQGEEEEEEEEDEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTSLQEFLRQLLTFYKVAGGCQERSQGCFPVYLVYSDKRMVQTAAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTPQHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVIAKTPGTGGSPQGSFADGQPAERRASNDQRPQEVPAEALAPAPAEVPAPAPAAASASGPAKTPAPAEASTSALVPEETPVEAPAPPPAEAPAQYPSEHLIQATSEENQIPSHLPACPSLRHVASLRGSAIIELFHSSIAEVENEELRHLMWSSVVFYQTPGLEVTACVLLSTKAVYFVLHDGLRRYFSEPLQDFWHQKNTDYNNSPFHISQCFVLKLSDLQSVNVGLFDQHFRLTGSTPMQVVTCLTRDSYLTHCFLQHLMVVLSSLERTPSPEPVDKDFYSEFGNKTTGKMENYELIHSSRVKFTYPSEEEIGDLTFTVAQKMAEPEKAPALSILLYVQAFQVGMPPPGCCRGPLRPKTLLLTSSEIFLLDEDCVHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYQTYPQALTLVFDDVQGHDLMGSVTLDHFGEVPGGPARASQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

Target ORF information:

RefSeq Version	NM_007184.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nischarin (NISCH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007184.3

ORF Insert Sequence:

ATGGCGACCG CGCGCACCTT CGGGCCCGAG CGGGAAGCCG AGCCGGCCAA GGAAGCGCGC 
GTCGTGGGCT CGGAGCTTGT GGACACTTAT ACGGTTTACA TCATCCAGGT CACTGATGGC 
AGCCATGAGT GGACAGTAAA GCACCGCTAC AGCGACTTCC ATGACCTGCA TGAAAAGCTC 
GTTGCAGAGA GAAAGATTGA TAAAAATCTG CTTCCGCCCA AAAAGATAAT TGGGAAAAAC 
TCAAGAAGCT TGGTGGAGAA GAGGGAGAAG GATCTGGAGG TCTACCTCCA GAAGCTCCTG 
GCTGCCTTCC CTGGCGTGAC CCCCAGAGTA CTGGCCCACT TCTTGCATTT TCACTTCTAT 
GAGATAAATG GCATCACCGC GGCACTGGCT GAAGAGCTCT TTGAGAAAGG AGAACAGCTC 
CTGGGGGCCG GCGAGGTCTT TGCCATTGGA CCCCTGCAGC TGTATGCCGT CACGGAGCAG 
CTGCAGCAGG GAAAGCCCAC GTGCGCCAGT GGGGATGCCA AGACCGACCT CGGGCACATC 
CTGGACTTCA CCTGTCGCCT TAAGTACCTT AAGGTTTCTG GCACAGAAGG ACCTTTTGGG 
ACCAGCAACA TTCAGGAGCA GCTCCTGCCG TTCGACCTAT CAATATTCAA GTCCCTGCAT 
CAGGTGGAGA TAAGTCACTG TGATGCTAAG CACATCAGAG GGCTGGTCGC ATCGAAGCCC 
ACCTTAGCCA CGCTGAGTGT CCGCTTCTCA GCAACCTCGA TGAAGGAAGT CCTTGTTCCT 
GAAGCCTCAG AATTTGATGA GTGGGAGCCT GAAGGCACAA CCCTAGAAGG CCCTGTGACT 
GCCGTCATCC CCACTTGGCA GGCATTGACC ACGCTTGACC TGAGCCACAA CAGCATCTCC 
GAGATCGACG AGTCTGTGAA ACTGATCCCA AAGATTGAGT TCCTGGACCT GAGTCACAAT 
GGATTGCTGG TTGTGGACAA TCTGCAGCAC CTGTATAACC TTGTGCATCT GGACCTGTCC 
TACAACAAGC TCTCCTCCTT GGAAGGGCTT CACACCAAGC TGGGGAACAT CAAGACCTTA 
AACCTGGCAG GCAACCTCCT AGAGAGTCTG AGTGGCCTGC ACAAGCTCTA CTCACTGGTC 
AACCTGGATC TCCGGGACAA CAGGATCGAA CAGATGGAGG AGGTCCGGAG CATAGGCAGC 
CTCCCGTGTC TGGAGCACGT GTCTCTGCTG AACAACCCTC TGAGCATCAT CCCCGACTAC 
CGGACCAAGG TGCTGGCTCA GTTCGGAGAG AGGGCCTCAG AGGTCTGTCT GGATGACACA 
GTGACCACAG AGAAGGAGCT GGACACTGTG GAAGTGCTGA AAGCAATTCA GAAAGCCAAG 
GAGGTCAAGT CCAAACTGAG CAACCCAGAG AAGAAGGGTG GTGAAGACTC CCGGCTCTCA 
GCTGCCCCCT GCATCAGACC CAGCAGCTCC CCTCCCACTG TGGCTCCCGC ATCTGCCTCC 
CTGCCCCAGC CCATCCTCTC TAACCAAGGA ATCATGTTCG TTCAGGAGGA GGCCCTGGCC 
AGCAGCCTCT CGTCCACTGA CAGTCTGACT CCCGAGCACC AGCCCATTGC CCAGGGATGT 
TCTGATTCCT TGGAGTCCAT CCCTGCGGGA CAGGCAGCTT CCGATGATTT AAGGGACGTG 
CCAGGAGCTG TTGGTGGTGC AAGCCCAGAA CATGCCGAGC CGGAGGTCCA GGTGGTGCCG 
GGGTCTGGCC AGATCATCTT CCTGCCCTTC ACCTGCATTG GCTACACGGC CACCAATCAG 
GACTTCATCC AGCGCCTGAG CACACTGATC CGGCAGGCCA TCGAGCGGCA GCTGCCTGCC 
TGGATCGAGG CTGCCAACCA GCGGGAGGAG GGCCAGGGTG AACAGGGCGA GGAGGAGGAT 
GAGGAGGAGG AAGAAGAGGA GGACGTGGCT GAGAACCGCT ACTTTGAAAT GGGGCCCCCA 
GACGTGGAGG AGGAGGAGGG AGGAGGCCAG GGGGAGGAAG AGGAGGAGGA AGAGGAGGAT 
GAAGAGGCCG AGGAGGAGCG CCTGGCTCTG GAATGGGCCC TGGGCGCGGA CGAGGACTTC 
CTGCTGGAGC ACATCCGCAT CCTCAAGGTG CTGTGGTGCT TCCTGATCCA TGTGCAGGGC 
AGTATCCGCC AGTTCGCCGC CTGCCTTGTG CTCACCGACT TCGGCATCGC AGTCTTCGAG 
ATCCCGCACC AGGAGTCTCG GGGCAGCAGC CAGCACATCC TCTCCTCCCT GCGCTTTGTC 
TTTTGCTTCC CGCATGGCGA CCTCACCGAG TTTGGCTTCC TCATGCCGGA GCTGTGTCTG 
GTGCTCAAGG TACGGCACAG TGAGAACACG CTCTTCATTA TCTCGGACGC CGCCAACCTG 
CACGAGTTCC ACGCGGACCT GCGCTCATGC TTTGCACCCC AGCACATGGC CATGCTGTGT 
AGCCCCATCC TCTACGGCAG CCACACCAGC CTGCAGGAGT TCCTGCGCCA GCTGCTCACC 
TTCTACAAGG TGGCTGGCGG CTGCCAGGAG CGCAGCCAGG GCTGCTTCCC CGTCTACCTG 
GTCTACAGTG ACAAGCGCAT GGTGCAGACG GCCGCCGGGG ACTACTCAGG CAACATCGAG 
TGGGCCAGCT GCACACTCTG TTCAGCCGTG CGGCGCTCCT GCTGCGCGCC CTCTGAGGCC 
GTCAAGTCCG CCGCCATCCC CTACTGGCTG TTGCTCACGC CCCAGCACCT CAACGTCATC 
AAGGCCGACT TCAACCCCAT GCCCAACCGT GGCACCCACA ACTGTCGCAA CCGCAACAGC 
TTCAAGCTCA GCCGTGTGCC GCTCTCCACC GTGCTGCTGG ACCCCACACG CAGCTGTACC 
CAGCCTCGGG GCGCCTTTGC TGATGGCCAC GTGCTAGAGC TGCTCGTGGG GTACCGCTTT 
GTCACTGCCA TCTTCGTGCT GCCCCACGAG AAGTTCCACT TCCTGCGCGT CTACAACCAG 
CTGCGGGCCT CGCTGCAGGA CCTGAAGACT GTGGTCATCG CCAAGACCCC CGGGACGGGA 
GGCAGCCCCC AGGGCTCCTT TGCGGATGGC CAGCCTGCCG AGCGCAGGGC CAGCAATGAC 
CAGCGTCCCC AGGAGGTCCC AGCAGAGGCT CTGGCCCCGG CCCCAGCGGA AGTCCCAGCT 
CCAGCCCCTG CAGCAGCCTC AGCCTCAGGC CCAGCGAAGA CTCCGGCCCC AGCAGAGGCC 
TCAACTTCAG CTTTGGTCCC AGAGGAGACG CCAGTGGAAG CTCCAGCCCC ACCCCCAGCC 
GAGGCCCCTG CCCAGTACCC GAGTGAGCAC CTCATCCAGG CCACCTCGGA GGAGAATCAG 
ATCCCCTCGC ACTTGCCTGC CTGCCCGTCG CTCCGGCACG TCGCCAGCCT GCGGGGCAGC 
GCCATCATCG AGCTCTTCCA CAGCAGCATT GCTGAGGTTG AAAACGAGGA GCTGAGGCAC 
CTCATGTGGT CCTCGGTGGT GTTCTACCAG ACCCCAGGGC TGGAGGTGAC TGCCTGCGTG 
CTGCTCTCCA CCAAGGCTGT GTACTTTGTG CTCCACGACG GCCTCCGCCG CTACTTCTCA 
GAGCCACTGC AGGATTTCTG GCATCAGAAA AACACCGACT ACAACAACAG CCCTTTCCAC 
ATCTCCCAGT GCTTCGTGCT AAAGCTTAGT GACCTGCAGT CAGTCAATGT GGGGCTTTTC 
GACCAGCATT TCCGGCTGAC GGGTTCCACC CCGATGCAGG TGGTCACGTG CTTGACGCGG 
GACAGCTACC TGACGCACTG CTTCCTCCAG CACCTCATGG TCGTGCTGTC CTCTCTGGAA 
CGCACGCCCT CGCCGGAGCC TGTTGACAAG GACTTCTACT CCGAGTTTGG GAACAAGACC 
ACAGGGAAGA TGGAGAACTA CGAGCTGATC CACTCTAGTC GCGTCAAGTT TACCTACCCC 
AGTGAGGAGG AGATTGGGGA CCTGACGTTC ACTGTGGCCC AAAAGATGGC TGAGCCAGAG 
AAGGCCCCAG CCCTCAGCAT CCTGCTGTAC GTGCAGGCCT TCCAGGTGGG CATGCCACCC 
CCTGGGTGCT GCAGGGGCCC CCTGCGCCCC AAGACACTCC TGCTCACCAG CTCCGAGATC 
TTCCTCCTGG ATGAGGACTG TGTCCACTAC CCACTGCCCG AGTTTGCCAA AGAGCCGCCG 
CAGAGAGACA GGTACCGGCT GGACGATGGC CGCCGCGTCC GGGACCTGGA CCGAGTGCTC 
ATGGGCTACC AGACCTACCC GCAGGCCCTC ACCCTCGTCT TCGATGACGT GCAAGGTCAT 
GACCTCATGG GCAGTGTCAC CCTGGACCAC TTTGGGGAGG TGCCAGGTGG CCCGGCTAGA 
GCCAGCCAGG GCCGTGAAGT CCAGTGGCAG GTGTTTGTCC CCAGTGCTGA GAGCAGAGAG 
AAGCTCATCT CGCTGTTGGC TCGCCAGTGG GAGGCCCTGT GTGGCCGTGA GCTGCCTGTC 
GAGCTCACCG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NISCH ( XM_017005643.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861132.1 Homo sapiens nischarin (NISCH), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu36939
Clone ID Related Accession (Same CDS sequence)	XM_017005643.1 , XM_005264839.2 , XM_017005643.2 , XM_005264839.3
Accession Version	XM_017005643.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nischarin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG TCCGGAGCAT AGGCAGCCTC CCGTGTCTGG AGCACGTGTC TCTGCTGAAC 
AACCCTCTGA GCATCATCCC CGACTACCGG ACCAAGGTGC TGGCTCAGTT CGGAGAGAGG 
GCCTCAGAGG TCTGTCTGGA TGACACAGTG ACCACAGAGA AGGAGCTGGA CACTGTGGAA 
GTGCTGAAAG CAATTCAGAA AGCCAAGGAG GTCAAGTCCA AACTGAGCAA CCCAGAGAAG 
AAGGGTGGTG AAGACTCCCG GCTCTCAGCT GCCCCCTGCA TCAGACCCAG CAGCTCCCCT 
CCCACTGTGG CTCCCGCATC TGCCTCCCTG CCCCAGCCCA TCCTCTCTAA CCAAGGAATC 
ATGTTCGTTC AGGAGGAGGC CCTGGCCAGC AGCCTCTCGT CCACTGACAG TCTGACTCCC 
GAGCACCAGC CCATTGCCCA GGGATGTTCT GATTCCTTGG AGTCCATCCC TGCGGGACAG 
GCAGCTTCCG ATGATTTAAG GGACGTGCCA GGAGCTGTTG GTGGTGCAAG CCCAGAACAT 
GCCGAGCCGG AGGTCCAGGT GGTGCCGGGG TCTGGCCAGA TCATCTTCCT GCCCTTCACC 
TGCATTGGCT ACACGGCCAC CAATCAGGAC TTCATCCAGC GCCTGAGCAC ACTGATCCGG 
CAGGCCATCG AGCGGCAGCT GCCTGCCTGG ATCGAGGCTG CCAACCAGCG GGAGGAGGGC 
CAGGGTGAAC AGGGCGAGGA GGAGGATGAG GAGGAGGAAG AAGAGGAGGA CGTGGCTGAG 
AACCGCTACT TTGAAATGGG GCCCCCAGAC GTGGAGGAGG AGGAGGGAGG AGGCCAGGGG 
GAGGAAGAGG AGGAGGAAGA GGAGGATGAA GAGGCCGAGG AGGAGCGCCT GGCTCTGGAA 
TGGGCCCTGG GCGCGGACGA GGACTTCCTG CTGGAGCACA TCCGCATCCT CAAGGTGCTG 
TGGTGCTTCC TGATCCATGT GCAGGGCAGT ATCCGCCAGT TCGCCGCCTG CCTTGTGCTC 
ACCGACTTCG GCATCGCAGT CTTCGAGATC CCGCACCAGG AGTCTCGGGG CAGCAGCCAG 
CACATCCTCT CCTCCCTGCG CTTTGTCTTT TGCTTCCCGC ATGGCGACCT CACCGAGTTT 
GGCTTCCTCA TGCCGGAGCT GTGTCTGGTG CTCAAGGTAC GGCACAGTGA GAACACGCTC 
TTCATTATCT CGGACGCCGC CAACCTGCAC GAGTTCCACG CGGACCTGCG CTCATGCTTT 
GCACCCCAGC ACATGGCCAT GCTGTGTAGC CCCATCCTCT ACGGCAGCCA CACCAGCCTG 
CAGGAGTTCC TGCGCCAGCT GCTCACCTTC TACAAGGTGG CTGGCGGCTG CCAGGAGCGC 
AGCCAGGGCT GCTTCCCCGT CTACCTGGTC TACAGTGACA AGCGCATGGT GCAGACGGCC 
GCCGGGGACT ACTCAGGCAA CATCGAGTGG GCCAGCTGCA CACTCTGTTC AGCCGTGCGG 
CGCTCCTGCT GCGCGCCCTC TGAGGCCGTC AAGTCCGCCG CCATCCCCTA CTGGCTGTTG 
CTCACGCCCC AGCACCTCAA CGTCATCAAG GCCGACTTCA ACCCCATGCC CAACCGTGGC 
ACCCACAACT GTCGCAACCG CAACAGCTTC AAGCTCAGCC GTGTGCCGCT CTCCACCGTG 
CTGCTGGACC CCACACGCAG CTGTACCCAG CCTCGGGGCG CCTTTGCTGA TGGCCACGTG 
CTAGAGCTGC TCGTGGGGTA CCGCTTTGTC ACTGCCATCT TCGTGCTGCC CCACGAGAAG 
TTCCACTTCC TGCGCGTCTA CAACCAGCTG CGGGCCTCGC TGCAGGACCT GAAGACTGTG 
GTCATCGCCA AGACCCCCGG GACGGGAGGC AGCCCCCAGG GCTCCTTTGC GGATGGCCAG 
CCTGCCGAGC GCAGGGCCAG CAATGACCAG CGTCCCCAGG AGGTCCCAGC AGAGGCTCTG 
GCCCCGGCCC CAGCGGAAGT CCCAGCTCCA GCCCCTGCAG CAGCCTCAGC CTCAGGCCCA 
GCGAAGACTC CGGCCCCAGC AGAGGCCTCA ACTTCAGCTT TGGTCCCAGA GGAGACGCCA 
GTGGAAGCTC CAGCCCCACC CCCAGCCGAG GCCCCTGCCC AGTACCCGAG TGAGCACCTC 
ATCCAGGCCA CCTCGGAGGA GAATCAGATC CCCTCGCACT TGCCTGCCTG CCCGTCGCTC 
CGGCACGTCG CCAGCCTGCG GGGCAGCGCC ATCATCGAGC TCTTCCACAG CAGCATTGCT 
GAGGTTGAAA ACGAGGAGCT GAGGCACCTC ATGTGGTCCT CGGTGGTGTT CTACCAGACC 
CCAGGGCTGG AGGTGACTGC CTGCGTGCTG CTCTCCACCA AGGCTGTGTA CTTTGTGCTC 
CACGACGGCC TCCGCCGCTA CTTCTCAGAG CCACTGCAGG ATTTCTGGCA TCAGAAAAAC 
ACCGACTACA ACAACAGCCC TTTCCACATC TCCCAGTGCT TCGTGCTAAA GCTTAGTGAC 
CTGCAGTCAG TCAATGTGGG GCTTTTCGAC CAGCATTTCC GGCTGACGGG TTCCACCCCG 
ATGCAGGTGG TCACGTGCTT GACGCGGGAC AGCTACCTGA CGCACTGCTT CCTCCAGCAC 
CTCATGGTCG TGCTGTCCTC TCTGGAACGC ACGCCCTCGC CGGAGCCTGT TGACAAGGAC 
TTCTACTCCG AGTTTGGGAA CAAGACCACA GGGAAGATGG AGAACTACGA GCTGATCCAC 
TCTAGTCGCG TCAAGTTTAC CTACCCCAGT GAGGAGGAGA TTGGGGACCT GACGTTCACT 
GTGGCCCAAA AGATGGCTGA GCCAGAGAAG GCCCCAGCCC TCAGCATCCT GCTGTACGTG 
CAGGCCTTCC AGGTGGGCAT GCCACCCCCT GGGTGCTGCA GGGGCCCCCT GCGCCCCAAG 
ACACTCCTGC TCACCAGCTC CGAGATCTTC CTCCTGGATG AGGACTGTGT CCACTACCCA 
CTGCCCGAGT TTGCCAAAGA GCCGCCGCAG AGAGACAGGT ACCGGCTGGA CGATGGCCGC 
CGCGTCCGGG ACCTGGACCG AGTGCTCATG GGCTACCAGA CCTACCCGCA GGCCCTCACC 
CTCGTCTTCG ATGACGTGCA AGGTCATGAC CTCATGGGCA GTGTCACCCT GGACCACTTT 
GGGGAGGTGC CAGGTGGCCC GGCTAGAGCC AGCCAGGGCC GTGAAGTCCA GTGGCAGGTG 
TTTGTCCCCA GTGCTGAGAG CAGAGAGAAG CTCATCTCGC TGTTGGCTCG CCAGTGGGAG 
GCCCTGTGTG GCCGTGAGCT GCCTGTCGAG CTCACCGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861132.1
CDS	149..3490
Translation	MEEVRSIGSLPCLEHVSLLNNPLSIIPDYRTKVLAQFGERASEVCLDDTVTTEKELDTVEVLKAIQKAKEVKSKLSNPEKKGGEDSRLSAAPCIRPSSSPPTVAPASASLPQPILSNQGIMFVQEEALASSLSSTDSLTPEHQPIAQGCSDSLESIPAGQAASDDLRDVPGAVGGASPEHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREEGQGEQGEEEDEEEEEEEDVAENRYFEMGPPDVEEEEGGGQGEEEEEEEEDEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTSLQEFLRQLLTFYKVAGGCQERSQGCFPVYLVYSDKRMVQTAAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTPQHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVIAKTPGTGGSPQGSFADGQPAERRASNDQRPQEVPAEALAPAPAEVPAPAPAAASASGPAKTPAPAEASTSALVPEETPVEAPAPPPAEAPAQYPSEHLIQATSEENQIPSHLPACPSLRHVASLRGSAIIELFHSSIAEVENEELRHLMWSSVVFYQTPGLEVTACVLLSTKAVYFVLHDGLRRYFSEPLQDFWHQKNTDYNNSPFHISQCFVLKLSDLQSVNVGLFDQHFRLTGSTPMQVVTCLTRDSYLTHCFLQHLMVVLSSLERTPSPEPVDKDFYSEFGNKTTGKMENYELIHSSRVKFTYPSEEEIGDLTFTVAQKMAEPEKAPALSILLYVQAFQVGMPPPGCCRGPLRPKTLLLTSSEIFLLDEDCVHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYQTYPQALTLVFDDVQGHDLMGSVTLDHFGEVPGGPARASQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

Target ORF information:

RefSeq Version	XM_017005643.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nischarin (NISCH), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005643.1

ORF Insert Sequence:

ATGGAGGAGG TCCGGAGCAT AGGCAGCCTC CCGTGTCTGG AGCACGTGTC TCTGCTGAAC 
AACCCTCTGA GCATCATCCC CGACTACCGG ACCAAGGTGC TGGCTCAGTT CGGAGAGAGG 
GCCTCAGAGG TCTGTCTGGA TGACACAGTG ACCACAGAGA AGGAGCTGGA CACTGTGGAA 
GTGCTGAAAG CAATTCAGAA AGCCAAGGAG GTCAAGTCCA AACTGAGCAA CCCAGAGAAG 
AAGGGTGGTG AAGACTCCCG GCTCTCAGCT GCCCCCTGCA TCAGACCCAG CAGCTCCCCT 
CCCACTGTGG CTCCCGCATC TGCCTCCCTG CCCCAGCCCA TCCTCTCTAA CCAAGGAATC 
ATGTTCGTTC AGGAGGAGGC CCTGGCCAGC AGCCTCTCGT CCACTGACAG TCTGACTCCC 
GAGCACCAGC CCATTGCCCA GGGATGTTCT GATTCCTTGG AGTCCATCCC TGCGGGACAG 
GCAGCTTCCG ATGATTTAAG GGACGTGCCA GGAGCTGTTG GTGGTGCAAG CCCAGAACAT 
GCCGAGCCGG AGGTCCAGGT GGTGCCGGGG TCTGGCCAGA TCATCTTCCT GCCCTTCACC 
TGCATTGGCT ACACGGCCAC CAATCAGGAC TTCATCCAGC GCCTGAGCAC ACTGATCCGG 
CAGGCCATCG AGCGGCAGCT GCCTGCCTGG ATCGAGGCTG CCAACCAGCG GGAGGAGGGC 
CAGGGTGAAC AGGGCGAGGA GGAGGATGAG GAGGAGGAAG AAGAGGAGGA CGTGGCTGAG 
AACCGCTACT TTGAAATGGG GCCCCCAGAC GTGGAGGAGG AGGAGGGAGG AGGCCAGGGG 
GAGGAAGAGG AGGAGGAAGA GGAGGATGAA GAGGCCGAGG AGGAGCGCCT GGCTCTGGAA 
TGGGCCCTGG GCGCGGACGA GGACTTCCTG CTGGAGCACA TCCGCATCCT CAAGGTGCTG 
TGGTGCTTCC TGATCCATGT GCAGGGCAGT ATCCGCCAGT TCGCCGCCTG CCTTGTGCTC 
ACCGACTTCG GCATCGCAGT CTTCGAGATC CCGCACCAGG AGTCTCGGGG CAGCAGCCAG 
CACATCCTCT CCTCCCTGCG CTTTGTCTTT TGCTTCCCGC ATGGCGACCT CACCGAGTTT 
GGCTTCCTCA TGCCGGAGCT GTGTCTGGTG CTCAAGGTAC GGCACAGTGA GAACACGCTC 
TTCATTATCT CGGACGCCGC CAACCTGCAC GAGTTCCACG CGGACCTGCG CTCATGCTTT 
GCACCCCAGC ACATGGCCAT GCTGTGTAGC CCCATCCTCT ACGGCAGCCA CACCAGCCTG 
CAGGAGTTCC TGCGCCAGCT GCTCACCTTC TACAAGGTGG CTGGCGGCTG CCAGGAGCGC 
AGCCAGGGCT GCTTCCCCGT CTACCTGGTC TACAGTGACA AGCGCATGGT GCAGACGGCC 
GCCGGGGACT ACTCAGGCAA CATCGAGTGG GCCAGCTGCA CACTCTGTTC AGCCGTGCGG 
CGCTCCTGCT GCGCGCCCTC TGAGGCCGTC AAGTCCGCCG CCATCCCCTA CTGGCTGTTG 
CTCACGCCCC AGCACCTCAA CGTCATCAAG GCCGACTTCA ACCCCATGCC CAACCGTGGC 
ACCCACAACT GTCGCAACCG CAACAGCTTC AAGCTCAGCC GTGTGCCGCT CTCCACCGTG 
CTGCTGGACC CCACACGCAG CTGTACCCAG CCTCGGGGCG CCTTTGCTGA TGGCCACGTG 
CTAGAGCTGC TCGTGGGGTA CCGCTTTGTC ACTGCCATCT TCGTGCTGCC CCACGAGAAG 
TTCCACTTCC TGCGCGTCTA CAACCAGCTG CGGGCCTCGC TGCAGGACCT GAAGACTGTG 
GTCATCGCCA AGACCCCCGG GACGGGAGGC AGCCCCCAGG GCTCCTTTGC GGATGGCCAG 
CCTGCCGAGC GCAGGGCCAG CAATGACCAG CGTCCCCAGG AGGTCCCAGC AGAGGCTCTG 
GCCCCGGCCC CAGCGGAAGT CCCAGCTCCA GCCCCTGCAG CAGCCTCAGC CTCAGGCCCA 
GCGAAGACTC CGGCCCCAGC AGAGGCCTCA ACTTCAGCTT TGGTCCCAGA GGAGACGCCA 
GTGGAAGCTC CAGCCCCACC CCCAGCCGAG GCCCCTGCCC AGTACCCGAG TGAGCACCTC 
ATCCAGGCCA CCTCGGAGGA GAATCAGATC CCCTCGCACT TGCCTGCCTG CCCGTCGCTC 
CGGCACGTCG CCAGCCTGCG GGGCAGCGCC ATCATCGAGC TCTTCCACAG CAGCATTGCT 
GAGGTTGAAA ACGAGGAGCT GAGGCACCTC ATGTGGTCCT CGGTGGTGTT CTACCAGACC 
CCAGGGCTGG AGGTGACTGC CTGCGTGCTG CTCTCCACCA AGGCTGTGTA CTTTGTGCTC 
CACGACGGCC TCCGCCGCTA CTTCTCAGAG CCACTGCAGG ATTTCTGGCA TCAGAAAAAC 
ACCGACTACA ACAACAGCCC TTTCCACATC TCCCAGTGCT TCGTGCTAAA GCTTAGTGAC 
CTGCAGTCAG TCAATGTGGG GCTTTTCGAC CAGCATTTCC GGCTGACGGG TTCCACCCCG 
ATGCAGGTGG TCACGTGCTT GACGCGGGAC AGCTACCTGA CGCACTGCTT CCTCCAGCAC 
CTCATGGTCG TGCTGTCCTC TCTGGAACGC ACGCCCTCGC CGGAGCCTGT TGACAAGGAC 
TTCTACTCCG AGTTTGGGAA CAAGACCACA GGGAAGATGG AGAACTACGA GCTGATCCAC 
TCTAGTCGCG TCAAGTTTAC CTACCCCAGT GAGGAGGAGA TTGGGGACCT GACGTTCACT 
GTGGCCCAAA AGATGGCTGA GCCAGAGAAG GCCCCAGCCC TCAGCATCCT GCTGTACGTG 
CAGGCCTTCC AGGTGGGCAT GCCACCCCCT GGGTGCTGCA GGGGCCCCCT GCGCCCCAAG 
ACACTCCTGC TCACCAGCTC CGAGATCTTC CTCCTGGATG AGGACTGTGT CCACTACCCA 
CTGCCCGAGT TTGCCAAAGA GCCGCCGCAG AGAGACAGGT ACCGGCTGGA CGATGGCCGC 
CGCGTCCGGG ACCTGGACCG AGTGCTCATG GGCTACCAGA CCTACCCGCA GGCCCTCACC 
CTCGTCTTCG ATGACGTGCA AGGTCATGAC CTCATGGGCA GTGTCACCCT GGACCACTTT 
GGGGAGGTGC CAGGTGGCCC GGCTAGAGCC AGCCAGGGCC GTGAAGTCCA GTGGCAGGTG 
TTTGTCCCCA GTGCTGAGAG CAGAGAGAAG CTCATCTCGC TGTTGGCTCG CCAGTGGGAG 
GCCCTGTGTG GCCGTGAGCT GCCTGTCGAG CTCACCGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NISCH ( XM_005264839.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264896.1 Homo sapiens nischarin (NISCH), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu36939
Clone ID Related Accession (Same CDS sequence)	XM_017005643.1 , XM_005264839.2 , XM_017005643.2 , XM_005264839.3
Accession Version	XM_005264839.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nischarin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005264839.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG TCCGGAGCAT AGGCAGCCTC CCGTGTCTGG AGCACGTGTC TCTGCTGAAC 
AACCCTCTGA GCATCATCCC CGACTACCGG ACCAAGGTGC TGGCTCAGTT CGGAGAGAGG 
GCCTCAGAGG TCTGTCTGGA TGACACAGTG ACCACAGAGA AGGAGCTGGA CACTGTGGAA 
GTGCTGAAAG CAATTCAGAA AGCCAAGGAG GTCAAGTCCA AACTGAGCAA CCCAGAGAAG 
AAGGGTGGTG AAGACTCCCG GCTCTCAGCT GCCCCCTGCA TCAGACCCAG CAGCTCCCCT 
CCCACTGTGG CTCCCGCATC TGCCTCCCTG CCCCAGCCCA TCCTCTCTAA CCAAGGAATC 
ATGTTCGTTC AGGAGGAGGC CCTGGCCAGC AGCCTCTCGT CCACTGACAG TCTGACTCCC 
GAGCACCAGC CCATTGCCCA GGGATGTTCT GATTCCTTGG AGTCCATCCC TGCGGGACAG 
GCAGCTTCCG ATGATTTAAG GGACGTGCCA GGAGCTGTTG GTGGTGCAAG CCCAGAACAT 
GCCGAGCCGG AGGTCCAGGT GGTGCCGGGG TCTGGCCAGA TCATCTTCCT GCCCTTCACC 
TGCATTGGCT ACACGGCCAC CAATCAGGAC TTCATCCAGC GCCTGAGCAC ACTGATCCGG 
CAGGCCATCG AGCGGCAGCT GCCTGCCTGG ATCGAGGCTG CCAACCAGCG GGAGGAGGGC 
CAGGGTGAAC AGGGCGAGGA GGAGGATGAG GAGGAGGAAG AAGAGGAGGA CGTGGCTGAG 
AACCGCTACT TTGAAATGGG GCCCCCAGAC GTGGAGGAGG AGGAGGGAGG AGGCCAGGGG 
GAGGAAGAGG AGGAGGAAGA GGAGGATGAA GAGGCCGAGG AGGAGCGCCT GGCTCTGGAA 
TGGGCCCTGG GCGCGGACGA GGACTTCCTG CTGGAGCACA TCCGCATCCT CAAGGTGCTG 
TGGTGCTTCC TGATCCATGT GCAGGGCAGT ATCCGCCAGT TCGCCGCCTG CCTTGTGCTC 
ACCGACTTCG GCATCGCAGT CTTCGAGATC CCGCACCAGG AGTCTCGGGG CAGCAGCCAG 
CACATCCTCT CCTCCCTGCG CTTTGTCTTT TGCTTCCCGC ATGGCGACCT CACCGAGTTT 
GGCTTCCTCA TGCCGGAGCT GTGTCTGGTG CTCAAGGTAC GGCACAGTGA GAACACGCTC 
TTCATTATCT CGGACGCCGC CAACCTGCAC GAGTTCCACG CGGACCTGCG CTCATGCTTT 
GCACCCCAGC ACATGGCCAT GCTGTGTAGC CCCATCCTCT ACGGCAGCCA CACCAGCCTG 
CAGGAGTTCC TGCGCCAGCT GCTCACCTTC TACAAGGTGG CTGGCGGCTG CCAGGAGCGC 
AGCCAGGGCT GCTTCCCCGT CTACCTGGTC TACAGTGACA AGCGCATGGT GCAGACGGCC 
GCCGGGGACT ACTCAGGCAA CATCGAGTGG GCCAGCTGCA CACTCTGTTC AGCCGTGCGG 
CGCTCCTGCT GCGCGCCCTC TGAGGCCGTC AAGTCCGCCG CCATCCCCTA CTGGCTGTTG 
CTCACGCCCC AGCACCTCAA CGTCATCAAG GCCGACTTCA ACCCCATGCC CAACCGTGGC 
ACCCACAACT GTCGCAACCG CAACAGCTTC AAGCTCAGCC GTGTGCCGCT CTCCACCGTG 
CTGCTGGACC CCACACGCAG CTGTACCCAG CCTCGGGGCG CCTTTGCTGA TGGCCACGTG 
CTAGAGCTGC TCGTGGGGTA CCGCTTTGTC ACTGCCATCT TCGTGCTGCC CCACGAGAAG 
TTCCACTTCC TGCGCGTCTA CAACCAGCTG CGGGCCTCGC TGCAGGACCT GAAGACTGTG 
GTCATCGCCA AGACCCCCGG GACGGGAGGC AGCCCCCAGG GCTCCTTTGC GGATGGCCAG 
CCTGCCGAGC GCAGGGCCAG CAATGACCAG CGTCCCCAGG AGGTCCCAGC AGAGGCTCTG 
GCCCCGGCCC CAGCGGAAGT CCCAGCTCCA GCCCCTGCAG CAGCCTCAGC CTCAGGCCCA 
GCGAAGACTC CGGCCCCAGC AGAGGCCTCA ACTTCAGCTT TGGTCCCAGA GGAGACGCCA 
GTGGAAGCTC CAGCCCCACC CCCAGCCGAG GCCCCTGCCC AGTACCCGAG TGAGCACCTC 
ATCCAGGCCA CCTCGGAGGA GAATCAGATC CCCTCGCACT TGCCTGCCTG CCCGTCGCTC 
CGGCACGTCG CCAGCCTGCG GGGCAGCGCC ATCATCGAGC TCTTCCACAG CAGCATTGCT 
GAGGTTGAAA ACGAGGAGCT GAGGCACCTC ATGTGGTCCT CGGTGGTGTT CTACCAGACC 
CCAGGGCTGG AGGTGACTGC CTGCGTGCTG CTCTCCACCA AGGCTGTGTA CTTTGTGCTC 
CACGACGGCC TCCGCCGCTA CTTCTCAGAG CCACTGCAGG ATTTCTGGCA TCAGAAAAAC 
ACCGACTACA ACAACAGCCC TTTCCACATC TCCCAGTGCT TCGTGCTAAA GCTTAGTGAC 
CTGCAGTCAG TCAATGTGGG GCTTTTCGAC CAGCATTTCC GGCTGACGGG TTCCACCCCG 
ATGCAGGTGG TCACGTGCTT GACGCGGGAC AGCTACCTGA CGCACTGCTT CCTCCAGCAC 
CTCATGGTCG TGCTGTCCTC TCTGGAACGC ACGCCCTCGC CGGAGCCTGT TGACAAGGAC 
TTCTACTCCG AGTTTGGGAA CAAGACCACA GGGAAGATGG AGAACTACGA GCTGATCCAC 
TCTAGTCGCG TCAAGTTTAC CTACCCCAGT GAGGAGGAGA TTGGGGACCT GACGTTCACT 
GTGGCCCAAA AGATGGCTGA GCCAGAGAAG GCCCCAGCCC TCAGCATCCT GCTGTACGTG 
CAGGCCTTCC AGGTGGGCAT GCCACCCCCT GGGTGCTGCA GGGGCCCCCT GCGCCCCAAG 
ACACTCCTGC TCACCAGCTC CGAGATCTTC CTCCTGGATG AGGACTGTGT CCACTACCCA 
CTGCCCGAGT TTGCCAAAGA GCCGCCGCAG AGAGACAGGT ACCGGCTGGA CGATGGCCGC 
CGCGTCCGGG ACCTGGACCG AGTGCTCATG GGCTACCAGA CCTACCCGCA GGCCCTCACC 
CTCGTCTTCG ATGACGTGCA AGGTCATGAC CTCATGGGCA GTGTCACCCT GGACCACTTT 
GGGGAGGTGC CAGGTGGCCC GGCTAGAGCC AGCCAGGGCC GTGAAGTCCA GTGGCAGGTG 
TTTGTCCCCA GTGCTGAGAG CAGAGAGAAG CTCATCTCGC TGTTGGCTCG CCAGTGGGAG 
GCCCTGTGTG GCCGTGAGCT GCCTGTCGAG CTCACCGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264896.1
CDS	1157..4498
Translation	MEEVRSIGSLPCLEHVSLLNNPLSIIPDYRTKVLAQFGERASEVCLDDTVTTEKELDTVEVLKAIQKAKEVKSKLSNPEKKGGEDSRLSAAPCIRPSSSPPTVAPASASLPQPILSNQGIMFVQEEALASSLSSTDSLTPEHQPIAQGCSDSLESIPAGQAASDDLRDVPGAVGGASPEHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREEGQGEQGEEEDEEEEEEEDVAENRYFEMGPPDVEEEEGGGQGEEEEEEEEDEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTSLQEFLRQLLTFYKVAGGCQERSQGCFPVYLVYSDKRMVQTAAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTPQHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVIAKTPGTGGSPQGSFADGQPAERRASNDQRPQEVPAEALAPAPAEVPAPAPAAASASGPAKTPAPAEASTSALVPEETPVEAPAPPPAEAPAQYPSEHLIQATSEENQIPSHLPACPSLRHVASLRGSAIIELFHSSIAEVENEELRHLMWSSVVFYQTPGLEVTACVLLSTKAVYFVLHDGLRRYFSEPLQDFWHQKNTDYNNSPFHISQCFVLKLSDLQSVNVGLFDQHFRLTGSTPMQVVTCLTRDSYLTHCFLQHLMVVLSSLERTPSPEPVDKDFYSEFGNKTTGKMENYELIHSSRVKFTYPSEEEIGDLTFTVAQKMAEPEKAPALSILLYVQAFQVGMPPPGCCRGPLRPKTLLLTSSEIFLLDEDCVHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYQTYPQALTLVFDDVQGHDLMGSVTLDHFGEVPGGPARASQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

Target ORF information:

RefSeq Version	XM_005264839.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nischarin (NISCH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264839.2

ORF Insert Sequence:

ATGGAGGAGG TCCGGAGCAT AGGCAGCCTC CCGTGTCTGG AGCACGTGTC TCTGCTGAAC 
AACCCTCTGA GCATCATCCC CGACTACCGG ACCAAGGTGC TGGCTCAGTT CGGAGAGAGG 
GCCTCAGAGG TCTGTCTGGA TGACACAGTG ACCACAGAGA AGGAGCTGGA CACTGTGGAA 
GTGCTGAAAG CAATTCAGAA AGCCAAGGAG GTCAAGTCCA AACTGAGCAA CCCAGAGAAG 
AAGGGTGGTG AAGACTCCCG GCTCTCAGCT GCCCCCTGCA TCAGACCCAG CAGCTCCCCT 
CCCACTGTGG CTCCCGCATC TGCCTCCCTG CCCCAGCCCA TCCTCTCTAA CCAAGGAATC 
ATGTTCGTTC AGGAGGAGGC CCTGGCCAGC AGCCTCTCGT CCACTGACAG TCTGACTCCC 
GAGCACCAGC CCATTGCCCA GGGATGTTCT GATTCCTTGG AGTCCATCCC TGCGGGACAG 
GCAGCTTCCG ATGATTTAAG GGACGTGCCA GGAGCTGTTG GTGGTGCAAG CCCAGAACAT 
GCCGAGCCGG AGGTCCAGGT GGTGCCGGGG TCTGGCCAGA TCATCTTCCT GCCCTTCACC 
TGCATTGGCT ACACGGCCAC CAATCAGGAC TTCATCCAGC GCCTGAGCAC ACTGATCCGG 
CAGGCCATCG AGCGGCAGCT GCCTGCCTGG ATCGAGGCTG CCAACCAGCG GGAGGAGGGC 
CAGGGTGAAC AGGGCGAGGA GGAGGATGAG GAGGAGGAAG AAGAGGAGGA CGTGGCTGAG 
AACCGCTACT TTGAAATGGG GCCCCCAGAC GTGGAGGAGG AGGAGGGAGG AGGCCAGGGG 
GAGGAAGAGG AGGAGGAAGA GGAGGATGAA GAGGCCGAGG AGGAGCGCCT GGCTCTGGAA 
TGGGCCCTGG GCGCGGACGA GGACTTCCTG CTGGAGCACA TCCGCATCCT CAAGGTGCTG 
TGGTGCTTCC TGATCCATGT GCAGGGCAGT ATCCGCCAGT TCGCCGCCTG CCTTGTGCTC 
ACCGACTTCG GCATCGCAGT CTTCGAGATC CCGCACCAGG AGTCTCGGGG CAGCAGCCAG 
CACATCCTCT CCTCCCTGCG CTTTGTCTTT TGCTTCCCGC ATGGCGACCT CACCGAGTTT 
GGCTTCCTCA TGCCGGAGCT GTGTCTGGTG CTCAAGGTAC GGCACAGTGA GAACACGCTC 
TTCATTATCT CGGACGCCGC CAACCTGCAC GAGTTCCACG CGGACCTGCG CTCATGCTTT 
GCACCCCAGC ACATGGCCAT GCTGTGTAGC CCCATCCTCT ACGGCAGCCA CACCAGCCTG 
CAGGAGTTCC TGCGCCAGCT GCTCACCTTC TACAAGGTGG CTGGCGGCTG CCAGGAGCGC 
AGCCAGGGCT GCTTCCCCGT CTACCTGGTC TACAGTGACA AGCGCATGGT GCAGACGGCC 
GCCGGGGACT ACTCAGGCAA CATCGAGTGG GCCAGCTGCA CACTCTGTTC AGCCGTGCGG 
CGCTCCTGCT GCGCGCCCTC TGAGGCCGTC AAGTCCGCCG CCATCCCCTA CTGGCTGTTG 
CTCACGCCCC AGCACCTCAA CGTCATCAAG GCCGACTTCA ACCCCATGCC CAACCGTGGC 
ACCCACAACT GTCGCAACCG CAACAGCTTC AAGCTCAGCC GTGTGCCGCT CTCCACCGTG 
CTGCTGGACC CCACACGCAG CTGTACCCAG CCTCGGGGCG CCTTTGCTGA TGGCCACGTG 
CTAGAGCTGC TCGTGGGGTA CCGCTTTGTC ACTGCCATCT TCGTGCTGCC CCACGAGAAG 
TTCCACTTCC TGCGCGTCTA CAACCAGCTG CGGGCCTCGC TGCAGGACCT GAAGACTGTG 
GTCATCGCCA AGACCCCCGG GACGGGAGGC AGCCCCCAGG GCTCCTTTGC GGATGGCCAG 
CCTGCCGAGC GCAGGGCCAG CAATGACCAG CGTCCCCAGG AGGTCCCAGC AGAGGCTCTG 
GCCCCGGCCC CAGCGGAAGT CCCAGCTCCA GCCCCTGCAG CAGCCTCAGC CTCAGGCCCA 
GCGAAGACTC CGGCCCCAGC AGAGGCCTCA ACTTCAGCTT TGGTCCCAGA GGAGACGCCA 
GTGGAAGCTC CAGCCCCACC CCCAGCCGAG GCCCCTGCCC AGTACCCGAG TGAGCACCTC 
ATCCAGGCCA CCTCGGAGGA GAATCAGATC CCCTCGCACT TGCCTGCCTG CCCGTCGCTC 
CGGCACGTCG CCAGCCTGCG GGGCAGCGCC ATCATCGAGC TCTTCCACAG CAGCATTGCT 
GAGGTTGAAA ACGAGGAGCT GAGGCACCTC ATGTGGTCCT CGGTGGTGTT CTACCAGACC 
CCAGGGCTGG AGGTGACTGC CTGCGTGCTG CTCTCCACCA AGGCTGTGTA CTTTGTGCTC 
CACGACGGCC TCCGCCGCTA CTTCTCAGAG CCACTGCAGG ATTTCTGGCA TCAGAAAAAC 
ACCGACTACA ACAACAGCCC TTTCCACATC TCCCAGTGCT TCGTGCTAAA GCTTAGTGAC 
CTGCAGTCAG TCAATGTGGG GCTTTTCGAC CAGCATTTCC GGCTGACGGG TTCCACCCCG 
ATGCAGGTGG TCACGTGCTT GACGCGGGAC AGCTACCTGA CGCACTGCTT CCTCCAGCAC 
CTCATGGTCG TGCTGTCCTC TCTGGAACGC ACGCCCTCGC CGGAGCCTGT TGACAAGGAC 
TTCTACTCCG AGTTTGGGAA CAAGACCACA GGGAAGATGG AGAACTACGA GCTGATCCAC 
TCTAGTCGCG TCAAGTTTAC CTACCCCAGT GAGGAGGAGA TTGGGGACCT GACGTTCACT 
GTGGCCCAAA AGATGGCTGA GCCAGAGAAG GCCCCAGCCC TCAGCATCCT GCTGTACGTG 
CAGGCCTTCC AGGTGGGCAT GCCACCCCCT GGGTGCTGCA GGGGCCCCCT GCGCCCCAAG 
ACACTCCTGC TCACCAGCTC CGAGATCTTC CTCCTGGATG AGGACTGTGT CCACTACCCA 
CTGCCCGAGT TTGCCAAAGA GCCGCCGCAG AGAGACAGGT ACCGGCTGGA CGATGGCCGC 
CGCGTCCGGG ACCTGGACCG AGTGCTCATG GGCTACCAGA CCTACCCGCA GGCCCTCACC 
CTCGTCTTCG ATGACGTGCA AGGTCATGAC CTCATGGGCA GTGTCACCCT GGACCACTTT 
GGGGAGGTGC CAGGTGGCCC GGCTAGAGCC AGCCAGGGCC GTGAAGTCCA GTGGCAGGTG 
TTTGTCCCCA GTGCTGAGAG CAGAGAGAAG CTCATCTCGC TGTTGGCTCG CCAGTGGGAG 
GCCCTGTGTG GCCGTGAGCT GCCTGTCGAG CTCACCGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NISCH ( XM_017005643.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861132.1 Homo sapiens nischarin (NISCH), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu36939
Clone ID Related Accession (Same CDS sequence)	XM_017005643.1 , XM_005264839.2 , XM_017005643.2 , XM_005264839.3
Accession Version	XM_017005643.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nischarin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005643.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG TCCGGAGCAT AGGCAGCCTC CCGTGTCTGG AGCACGTGTC TCTGCTGAAC 
AACCCTCTGA GCATCATCCC CGACTACCGG ACCAAGGTGC TGGCTCAGTT CGGAGAGAGG 
GCCTCAGAGG TCTGTCTGGA TGACACAGTG ACCACAGAGA AGGAGCTGGA CACTGTGGAA 
GTGCTGAAAG CAATTCAGAA AGCCAAGGAG GTCAAGTCCA AACTGAGCAA CCCAGAGAAG 
AAGGGTGGTG AAGACTCCCG GCTCTCAGCT GCCCCCTGCA TCAGACCCAG CAGCTCCCCT 
CCCACTGTGG CTCCCGCATC TGCCTCCCTG CCCCAGCCCA TCCTCTCTAA CCAAGGAATC 
ATGTTCGTTC AGGAGGAGGC CCTGGCCAGC AGCCTCTCGT CCACTGACAG TCTGACTCCC 
GAGCACCAGC CCATTGCCCA GGGATGTTCT GATTCCTTGG AGTCCATCCC TGCGGGACAG 
GCAGCTTCCG ATGATTTAAG GGACGTGCCA GGAGCTGTTG GTGGTGCAAG CCCAGAACAT 
GCCGAGCCGG AGGTCCAGGT GGTGCCGGGG TCTGGCCAGA TCATCTTCCT GCCCTTCACC 
TGCATTGGCT ACACGGCCAC CAATCAGGAC TTCATCCAGC GCCTGAGCAC ACTGATCCGG 
CAGGCCATCG AGCGGCAGCT GCCTGCCTGG ATCGAGGCTG CCAACCAGCG GGAGGAGGGC 
CAGGGTGAAC AGGGCGAGGA GGAGGATGAG GAGGAGGAAG AAGAGGAGGA CGTGGCTGAG 
AACCGCTACT TTGAAATGGG GCCCCCAGAC GTGGAGGAGG AGGAGGGAGG AGGCCAGGGG 
GAGGAAGAGG AGGAGGAAGA GGAGGATGAA GAGGCCGAGG AGGAGCGCCT GGCTCTGGAA 
TGGGCCCTGG GCGCGGACGA GGACTTCCTG CTGGAGCACA TCCGCATCCT CAAGGTGCTG 
TGGTGCTTCC TGATCCATGT GCAGGGCAGT ATCCGCCAGT TCGCCGCCTG CCTTGTGCTC 
ACCGACTTCG GCATCGCAGT CTTCGAGATC CCGCACCAGG AGTCTCGGGG CAGCAGCCAG 
CACATCCTCT CCTCCCTGCG CTTTGTCTTT TGCTTCCCGC ATGGCGACCT CACCGAGTTT 
GGCTTCCTCA TGCCGGAGCT GTGTCTGGTG CTCAAGGTAC GGCACAGTGA GAACACGCTC 
TTCATTATCT CGGACGCCGC CAACCTGCAC GAGTTCCACG CGGACCTGCG CTCATGCTTT 
GCACCCCAGC ACATGGCCAT GCTGTGTAGC CCCATCCTCT ACGGCAGCCA CACCAGCCTG 
CAGGAGTTCC TGCGCCAGCT GCTCACCTTC TACAAGGTGG CTGGCGGCTG CCAGGAGCGC 
AGCCAGGGCT GCTTCCCCGT CTACCTGGTC TACAGTGACA AGCGCATGGT GCAGACGGCC 
GCCGGGGACT ACTCAGGCAA CATCGAGTGG GCCAGCTGCA CACTCTGTTC AGCCGTGCGG 
CGCTCCTGCT GCGCGCCCTC TGAGGCCGTC AAGTCCGCCG CCATCCCCTA CTGGCTGTTG 
CTCACGCCCC AGCACCTCAA CGTCATCAAG GCCGACTTCA ACCCCATGCC CAACCGTGGC 
ACCCACAACT GTCGCAACCG CAACAGCTTC AAGCTCAGCC GTGTGCCGCT CTCCACCGTG 
CTGCTGGACC CCACACGCAG CTGTACCCAG CCTCGGGGCG CCTTTGCTGA TGGCCACGTG 
CTAGAGCTGC TCGTGGGGTA CCGCTTTGTC ACTGCCATCT TCGTGCTGCC CCACGAGAAG 
TTCCACTTCC TGCGCGTCTA CAACCAGCTG CGGGCCTCGC TGCAGGACCT GAAGACTGTG 
GTCATCGCCA AGACCCCCGG GACGGGAGGC AGCCCCCAGG GCTCCTTTGC GGATGGCCAG 
CCTGCCGAGC GCAGGGCCAG CAATGACCAG CGTCCCCAGG AGGTCCCAGC AGAGGCTCTG 
GCCCCGGCCC CAGCGGAAGT CCCAGCTCCA GCCCCTGCAG CAGCCTCAGC CTCAGGCCCA 
GCGAAGACTC CGGCCCCAGC AGAGGCCTCA ACTTCAGCTT TGGTCCCAGA GGAGACGCCA 
GTGGAAGCTC CAGCCCCACC CCCAGCCGAG GCCCCTGCCC AGTACCCGAG TGAGCACCTC 
ATCCAGGCCA CCTCGGAGGA GAATCAGATC CCCTCGCACT TGCCTGCCTG CCCGTCGCTC 
CGGCACGTCG CCAGCCTGCG GGGCAGCGCC ATCATCGAGC TCTTCCACAG CAGCATTGCT 
GAGGTTGAAA ACGAGGAGCT GAGGCACCTC ATGTGGTCCT CGGTGGTGTT CTACCAGACC 
CCAGGGCTGG AGGTGACTGC CTGCGTGCTG CTCTCCACCA AGGCTGTGTA CTTTGTGCTC 
CACGACGGCC TCCGCCGCTA CTTCTCAGAG CCACTGCAGG ATTTCTGGCA TCAGAAAAAC 
ACCGACTACA ACAACAGCCC TTTCCACATC TCCCAGTGCT TCGTGCTAAA GCTTAGTGAC 
CTGCAGTCAG TCAATGTGGG GCTTTTCGAC CAGCATTTCC GGCTGACGGG TTCCACCCCG 
ATGCAGGTGG TCACGTGCTT GACGCGGGAC AGCTACCTGA CGCACTGCTT CCTCCAGCAC 
CTCATGGTCG TGCTGTCCTC TCTGGAACGC ACGCCCTCGC CGGAGCCTGT TGACAAGGAC 
TTCTACTCCG AGTTTGGGAA CAAGACCACA GGGAAGATGG AGAACTACGA GCTGATCCAC 
TCTAGTCGCG TCAAGTTTAC CTACCCCAGT GAGGAGGAGA TTGGGGACCT GACGTTCACT 
GTGGCCCAAA AGATGGCTGA GCCAGAGAAG GCCCCAGCCC TCAGCATCCT GCTGTACGTG 
CAGGCCTTCC AGGTGGGCAT GCCACCCCCT GGGTGCTGCA GGGGCCCCCT GCGCCCCAAG 
ACACTCCTGC TCACCAGCTC CGAGATCTTC CTCCTGGATG AGGACTGTGT CCACTACCCA 
CTGCCCGAGT TTGCCAAAGA GCCGCCGCAG AGAGACAGGT ACCGGCTGGA CGATGGCCGC 
CGCGTCCGGG ACCTGGACCG AGTGCTCATG GGCTACCAGA CCTACCCGCA GGCCCTCACC 
CTCGTCTTCG ATGACGTGCA AGGTCATGAC CTCATGGGCA GTGTCACCCT GGACCACTTT 
GGGGAGGTGC CAGGTGGCCC GGCTAGAGCC AGCCAGGGCC GTGAAGTCCA GTGGCAGGTG 
TTTGTCCCCA GTGCTGAGAG CAGAGAGAAG CTCATCTCGC TGTTGGCTCG CCAGTGGGAG 
GCCCTGTGTG GCCGTGAGCT GCCTGTCGAG CTCACCGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861132.1
CDS	148..3489
Translation	MEEVRSIGSLPCLEHVSLLNNPLSIIPDYRTKVLAQFGERASEVCLDDTVTTEKELDTVEVLKAIQKAKEVKSKLSNPEKKGGEDSRLSAAPCIRPSSSPPTVAPASASLPQPILSNQGIMFVQEEALASSLSSTDSLTPEHQPIAQGCSDSLESIPAGQAASDDLRDVPGAVGGASPEHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREEGQGEQGEEEDEEEEEEEDVAENRYFEMGPPDVEEEEGGGQGEEEEEEEEDEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTSLQEFLRQLLTFYKVAGGCQERSQGCFPVYLVYSDKRMVQTAAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTPQHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVIAKTPGTGGSPQGSFADGQPAERRASNDQRPQEVPAEALAPAPAEVPAPAPAAASASGPAKTPAPAEASTSALVPEETPVEAPAPPPAEAPAQYPSEHLIQATSEENQIPSHLPACPSLRHVASLRGSAIIELFHSSIAEVENEELRHLMWSSVVFYQTPGLEVTACVLLSTKAVYFVLHDGLRRYFSEPLQDFWHQKNTDYNNSPFHISQCFVLKLSDLQSVNVGLFDQHFRLTGSTPMQVVTCLTRDSYLTHCFLQHLMVVLSSLERTPSPEPVDKDFYSEFGNKTTGKMENYELIHSSRVKFTYPSEEEIGDLTFTVAQKMAEPEKAPALSILLYVQAFQVGMPPPGCCRGPLRPKTLLLTSSEIFLLDEDCVHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYQTYPQALTLVFDDVQGHDLMGSVTLDHFGEVPGGPARASQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

Target ORF information:

RefSeq Version	XM_017005643.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nischarin (NISCH), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005643.2

ORF Insert Sequence:

ATGGAGGAGG TCCGGAGCAT AGGCAGCCTC CCGTGTCTGG AGCACGTGTC TCTGCTGAAC 
AACCCTCTGA GCATCATCCC CGACTACCGG ACCAAGGTGC TGGCTCAGTT CGGAGAGAGG 
GCCTCAGAGG TCTGTCTGGA TGACACAGTG ACCACAGAGA AGGAGCTGGA CACTGTGGAA 
GTGCTGAAAG CAATTCAGAA AGCCAAGGAG GTCAAGTCCA AACTGAGCAA CCCAGAGAAG 
AAGGGTGGTG AAGACTCCCG GCTCTCAGCT GCCCCCTGCA TCAGACCCAG CAGCTCCCCT 
CCCACTGTGG CTCCCGCATC TGCCTCCCTG CCCCAGCCCA TCCTCTCTAA CCAAGGAATC 
ATGTTCGTTC AGGAGGAGGC CCTGGCCAGC AGCCTCTCGT CCACTGACAG TCTGACTCCC 
GAGCACCAGC CCATTGCCCA GGGATGTTCT GATTCCTTGG AGTCCATCCC TGCGGGACAG 
GCAGCTTCCG ATGATTTAAG GGACGTGCCA GGAGCTGTTG GTGGTGCAAG CCCAGAACAT 
GCCGAGCCGG AGGTCCAGGT GGTGCCGGGG TCTGGCCAGA TCATCTTCCT GCCCTTCACC 
TGCATTGGCT ACACGGCCAC CAATCAGGAC TTCATCCAGC GCCTGAGCAC ACTGATCCGG 
CAGGCCATCG AGCGGCAGCT GCCTGCCTGG ATCGAGGCTG CCAACCAGCG GGAGGAGGGC 
CAGGGTGAAC AGGGCGAGGA GGAGGATGAG GAGGAGGAAG AAGAGGAGGA CGTGGCTGAG 
AACCGCTACT TTGAAATGGG GCCCCCAGAC GTGGAGGAGG AGGAGGGAGG AGGCCAGGGG 
GAGGAAGAGG AGGAGGAAGA GGAGGATGAA GAGGCCGAGG AGGAGCGCCT GGCTCTGGAA 
TGGGCCCTGG GCGCGGACGA GGACTTCCTG CTGGAGCACA TCCGCATCCT CAAGGTGCTG 
TGGTGCTTCC TGATCCATGT GCAGGGCAGT ATCCGCCAGT TCGCCGCCTG CCTTGTGCTC 
ACCGACTTCG GCATCGCAGT CTTCGAGATC CCGCACCAGG AGTCTCGGGG CAGCAGCCAG 
CACATCCTCT CCTCCCTGCG CTTTGTCTTT TGCTTCCCGC ATGGCGACCT CACCGAGTTT 
GGCTTCCTCA TGCCGGAGCT GTGTCTGGTG CTCAAGGTAC GGCACAGTGA GAACACGCTC 
TTCATTATCT CGGACGCCGC CAACCTGCAC GAGTTCCACG CGGACCTGCG CTCATGCTTT 
GCACCCCAGC ACATGGCCAT GCTGTGTAGC CCCATCCTCT ACGGCAGCCA CACCAGCCTG 
CAGGAGTTCC TGCGCCAGCT GCTCACCTTC TACAAGGTGG CTGGCGGCTG CCAGGAGCGC 
AGCCAGGGCT GCTTCCCCGT CTACCTGGTC TACAGTGACA AGCGCATGGT GCAGACGGCC 
GCCGGGGACT ACTCAGGCAA CATCGAGTGG GCCAGCTGCA CACTCTGTTC AGCCGTGCGG 
CGCTCCTGCT GCGCGCCCTC TGAGGCCGTC AAGTCCGCCG CCATCCCCTA CTGGCTGTTG 
CTCACGCCCC AGCACCTCAA CGTCATCAAG GCCGACTTCA ACCCCATGCC CAACCGTGGC 
ACCCACAACT GTCGCAACCG CAACAGCTTC AAGCTCAGCC GTGTGCCGCT CTCCACCGTG 
CTGCTGGACC CCACACGCAG CTGTACCCAG CCTCGGGGCG CCTTTGCTGA TGGCCACGTG 
CTAGAGCTGC TCGTGGGGTA CCGCTTTGTC ACTGCCATCT TCGTGCTGCC CCACGAGAAG 
TTCCACTTCC TGCGCGTCTA CAACCAGCTG CGGGCCTCGC TGCAGGACCT GAAGACTGTG 
GTCATCGCCA AGACCCCCGG GACGGGAGGC AGCCCCCAGG GCTCCTTTGC GGATGGCCAG 
CCTGCCGAGC GCAGGGCCAG CAATGACCAG CGTCCCCAGG AGGTCCCAGC AGAGGCTCTG 
GCCCCGGCCC CAGCGGAAGT CCCAGCTCCA GCCCCTGCAG CAGCCTCAGC CTCAGGCCCA 
GCGAAGACTC CGGCCCCAGC AGAGGCCTCA ACTTCAGCTT TGGTCCCAGA GGAGACGCCA 
GTGGAAGCTC CAGCCCCACC CCCAGCCGAG GCCCCTGCCC AGTACCCGAG TGAGCACCTC 
ATCCAGGCCA CCTCGGAGGA GAATCAGATC CCCTCGCACT TGCCTGCCTG CCCGTCGCTC 
CGGCACGTCG CCAGCCTGCG GGGCAGCGCC ATCATCGAGC TCTTCCACAG CAGCATTGCT 
GAGGTTGAAA ACGAGGAGCT GAGGCACCTC ATGTGGTCCT CGGTGGTGTT CTACCAGACC 
CCAGGGCTGG AGGTGACTGC CTGCGTGCTG CTCTCCACCA AGGCTGTGTA CTTTGTGCTC 
CACGACGGCC TCCGCCGCTA CTTCTCAGAG CCACTGCAGG ATTTCTGGCA TCAGAAAAAC 
ACCGACTACA ACAACAGCCC TTTCCACATC TCCCAGTGCT TCGTGCTAAA GCTTAGTGAC 
CTGCAGTCAG TCAATGTGGG GCTTTTCGAC CAGCATTTCC GGCTGACGGG TTCCACCCCG 
ATGCAGGTGG TCACGTGCTT GACGCGGGAC AGCTACCTGA CGCACTGCTT CCTCCAGCAC 
CTCATGGTCG TGCTGTCCTC TCTGGAACGC ACGCCCTCGC CGGAGCCTGT TGACAAGGAC 
TTCTACTCCG AGTTTGGGAA CAAGACCACA GGGAAGATGG AGAACTACGA GCTGATCCAC 
TCTAGTCGCG TCAAGTTTAC CTACCCCAGT GAGGAGGAGA TTGGGGACCT GACGTTCACT 
GTGGCCCAAA AGATGGCTGA GCCAGAGAAG GCCCCAGCCC TCAGCATCCT GCTGTACGTG 
CAGGCCTTCC AGGTGGGCAT GCCACCCCCT GGGTGCTGCA GGGGCCCCCT GCGCCCCAAG 
ACACTCCTGC TCACCAGCTC CGAGATCTTC CTCCTGGATG AGGACTGTGT CCACTACCCA 
CTGCCCGAGT TTGCCAAAGA GCCGCCGCAG AGAGACAGGT ACCGGCTGGA CGATGGCCGC 
CGCGTCCGGG ACCTGGACCG AGTGCTCATG GGCTACCAGA CCTACCCGCA GGCCCTCACC 
CTCGTCTTCG ATGACGTGCA AGGTCATGAC CTCATGGGCA GTGTCACCCT GGACCACTTT 
GGGGAGGTGC CAGGTGGCCC GGCTAGAGCC AGCCAGGGCC GTGAAGTCCA GTGGCAGGTG 
TTTGTCCCCA GTGCTGAGAG CAGAGAGAAG CTCATCTCGC TGTTGGCTCG CCAGTGGGAG 
GCCCTGTGTG GCCGTGAGCT GCCTGTCGAG CTCACCGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NISCH ( XM_005264839.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264896.1 Homo sapiens nischarin (NISCH), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu36939
Clone ID Related Accession (Same CDS sequence)	XM_017005643.1 , XM_005264839.2 , XM_017005643.2 , XM_005264839.3
Accession Version	XM_005264839.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nischarin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005264839.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG TCCGGAGCAT AGGCAGCCTC CCGTGTCTGG AGCACGTGTC TCTGCTGAAC 
AACCCTCTGA GCATCATCCC CGACTACCGG ACCAAGGTGC TGGCTCAGTT CGGAGAGAGG 
GCCTCAGAGG TCTGTCTGGA TGACACAGTG ACCACAGAGA AGGAGCTGGA CACTGTGGAA 
GTGCTGAAAG CAATTCAGAA AGCCAAGGAG GTCAAGTCCA AACTGAGCAA CCCAGAGAAG 
AAGGGTGGTG AAGACTCCCG GCTCTCAGCT GCCCCCTGCA TCAGACCCAG CAGCTCCCCT 
CCCACTGTGG CTCCCGCATC TGCCTCCCTG CCCCAGCCCA TCCTCTCTAA CCAAGGAATC 
ATGTTCGTTC AGGAGGAGGC CCTGGCCAGC AGCCTCTCGT CCACTGACAG TCTGACTCCC 
GAGCACCAGC CCATTGCCCA GGGATGTTCT GATTCCTTGG AGTCCATCCC TGCGGGACAG 
GCAGCTTCCG ATGATTTAAG GGACGTGCCA GGAGCTGTTG GTGGTGCAAG CCCAGAACAT 
GCCGAGCCGG AGGTCCAGGT GGTGCCGGGG TCTGGCCAGA TCATCTTCCT GCCCTTCACC 
TGCATTGGCT ACACGGCCAC CAATCAGGAC TTCATCCAGC GCCTGAGCAC ACTGATCCGG 
CAGGCCATCG AGCGGCAGCT GCCTGCCTGG ATCGAGGCTG CCAACCAGCG GGAGGAGGGC 
CAGGGTGAAC AGGGCGAGGA GGAGGATGAG GAGGAGGAAG AAGAGGAGGA CGTGGCTGAG 
AACCGCTACT TTGAAATGGG GCCCCCAGAC GTGGAGGAGG AGGAGGGAGG AGGCCAGGGG 
GAGGAAGAGG AGGAGGAAGA GGAGGATGAA GAGGCCGAGG AGGAGCGCCT GGCTCTGGAA 
TGGGCCCTGG GCGCGGACGA GGACTTCCTG CTGGAGCACA TCCGCATCCT CAAGGTGCTG 
TGGTGCTTCC TGATCCATGT GCAGGGCAGT ATCCGCCAGT TCGCCGCCTG CCTTGTGCTC 
ACCGACTTCG GCATCGCAGT CTTCGAGATC CCGCACCAGG AGTCTCGGGG CAGCAGCCAG 
CACATCCTCT CCTCCCTGCG CTTTGTCTTT TGCTTCCCGC ATGGCGACCT CACCGAGTTT 
GGCTTCCTCA TGCCGGAGCT GTGTCTGGTG CTCAAGGTAC GGCACAGTGA GAACACGCTC 
TTCATTATCT CGGACGCCGC CAACCTGCAC GAGTTCCACG CGGACCTGCG CTCATGCTTT 
GCACCCCAGC ACATGGCCAT GCTGTGTAGC CCCATCCTCT ACGGCAGCCA CACCAGCCTG 
CAGGAGTTCC TGCGCCAGCT GCTCACCTTC TACAAGGTGG CTGGCGGCTG CCAGGAGCGC 
AGCCAGGGCT GCTTCCCCGT CTACCTGGTC TACAGTGACA AGCGCATGGT GCAGACGGCC 
GCCGGGGACT ACTCAGGCAA CATCGAGTGG GCCAGCTGCA CACTCTGTTC AGCCGTGCGG 
CGCTCCTGCT GCGCGCCCTC TGAGGCCGTC AAGTCCGCCG CCATCCCCTA CTGGCTGTTG 
CTCACGCCCC AGCACCTCAA CGTCATCAAG GCCGACTTCA ACCCCATGCC CAACCGTGGC 
ACCCACAACT GTCGCAACCG CAACAGCTTC AAGCTCAGCC GTGTGCCGCT CTCCACCGTG 
CTGCTGGACC CCACACGCAG CTGTACCCAG CCTCGGGGCG CCTTTGCTGA TGGCCACGTG 
CTAGAGCTGC TCGTGGGGTA CCGCTTTGTC ACTGCCATCT TCGTGCTGCC CCACGAGAAG 
TTCCACTTCC TGCGCGTCTA CAACCAGCTG CGGGCCTCGC TGCAGGACCT GAAGACTGTG 
GTCATCGCCA AGACCCCCGG GACGGGAGGC AGCCCCCAGG GCTCCTTTGC GGATGGCCAG 
CCTGCCGAGC GCAGGGCCAG CAATGACCAG CGTCCCCAGG AGGTCCCAGC AGAGGCTCTG 
GCCCCGGCCC CAGCGGAAGT CCCAGCTCCA GCCCCTGCAG CAGCCTCAGC CTCAGGCCCA 
GCGAAGACTC CGGCCCCAGC AGAGGCCTCA ACTTCAGCTT TGGTCCCAGA GGAGACGCCA 
GTGGAAGCTC CAGCCCCACC CCCAGCCGAG GCCCCTGCCC AGTACCCGAG TGAGCACCTC 
ATCCAGGCCA CCTCGGAGGA GAATCAGATC CCCTCGCACT TGCCTGCCTG CCCGTCGCTC 
CGGCACGTCG CCAGCCTGCG GGGCAGCGCC ATCATCGAGC TCTTCCACAG CAGCATTGCT 
GAGGTTGAAA ACGAGGAGCT GAGGCACCTC ATGTGGTCCT CGGTGGTGTT CTACCAGACC 
CCAGGGCTGG AGGTGACTGC CTGCGTGCTG CTCTCCACCA AGGCTGTGTA CTTTGTGCTC 
CACGACGGCC TCCGCCGCTA CTTCTCAGAG CCACTGCAGG ATTTCTGGCA TCAGAAAAAC 
ACCGACTACA ACAACAGCCC TTTCCACATC TCCCAGTGCT TCGTGCTAAA GCTTAGTGAC 
CTGCAGTCAG TCAATGTGGG GCTTTTCGAC CAGCATTTCC GGCTGACGGG TTCCACCCCG 
ATGCAGGTGG TCACGTGCTT GACGCGGGAC AGCTACCTGA CGCACTGCTT CCTCCAGCAC 
CTCATGGTCG TGCTGTCCTC TCTGGAACGC ACGCCCTCGC CGGAGCCTGT TGACAAGGAC 
TTCTACTCCG AGTTTGGGAA CAAGACCACA GGGAAGATGG AGAACTACGA GCTGATCCAC 
TCTAGTCGCG TCAAGTTTAC CTACCCCAGT GAGGAGGAGA TTGGGGACCT GACGTTCACT 
GTGGCCCAAA AGATGGCTGA GCCAGAGAAG GCCCCAGCCC TCAGCATCCT GCTGTACGTG 
CAGGCCTTCC AGGTGGGCAT GCCACCCCCT GGGTGCTGCA GGGGCCCCCT GCGCCCCAAG 
ACACTCCTGC TCACCAGCTC CGAGATCTTC CTCCTGGATG AGGACTGTGT CCACTACCCA 
CTGCCCGAGT TTGCCAAAGA GCCGCCGCAG AGAGACAGGT ACCGGCTGGA CGATGGCCGC 
CGCGTCCGGG ACCTGGACCG AGTGCTCATG GGCTACCAGA CCTACCCGCA GGCCCTCACC 
CTCGTCTTCG ATGACGTGCA AGGTCATGAC CTCATGGGCA GTGTCACCCT GGACCACTTT 
GGGGAGGTGC CAGGTGGCCC GGCTAGAGCC AGCCAGGGCC GTGAAGTCCA GTGGCAGGTG 
TTTGTCCCCA GTGCTGAGAG CAGAGAGAAG CTCATCTCGC TGTTGGCTCG CCAGTGGGAG 
GCCCTGTGTG GCCGTGAGCT GCCTGTCGAG CTCACCGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264896.1
CDS	1270..4611
Translation	MEEVRSIGSLPCLEHVSLLNNPLSIIPDYRTKVLAQFGERASEVCLDDTVTTEKELDTVEVLKAIQKAKEVKSKLSNPEKKGGEDSRLSAAPCIRPSSSPPTVAPASASLPQPILSNQGIMFVQEEALASSLSSTDSLTPEHQPIAQGCSDSLESIPAGQAASDDLRDVPGAVGGASPEHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREEGQGEQGEEEDEEEEEEEDVAENRYFEMGPPDVEEEEGGGQGEEEEEEEEDEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTSLQEFLRQLLTFYKVAGGCQERSQGCFPVYLVYSDKRMVQTAAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTPQHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVIAKTPGTGGSPQGSFADGQPAERRASNDQRPQEVPAEALAPAPAEVPAPAPAAASASGPAKTPAPAEASTSALVPEETPVEAPAPPPAEAPAQYPSEHLIQATSEENQIPSHLPACPSLRHVASLRGSAIIELFHSSIAEVENEELRHLMWSSVVFYQTPGLEVTACVLLSTKAVYFVLHDGLRRYFSEPLQDFWHQKNTDYNNSPFHISQCFVLKLSDLQSVNVGLFDQHFRLTGSTPMQVVTCLTRDSYLTHCFLQHLMVVLSSLERTPSPEPVDKDFYSEFGNKTTGKMENYELIHSSRVKFTYPSEEEIGDLTFTVAQKMAEPEKAPALSILLYVQAFQVGMPPPGCCRGPLRPKTLLLTSSEIFLLDEDCVHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYQTYPQALTLVFDDVQGHDLMGSVTLDHFGEVPGGPARASQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

Target ORF information:

RefSeq Version	XM_005264839.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nischarin (NISCH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264839.3

ORF Insert Sequence:

ATGGAGGAGG TCCGGAGCAT AGGCAGCCTC CCGTGTCTGG AGCACGTGTC TCTGCTGAAC 
AACCCTCTGA GCATCATCCC CGACTACCGG ACCAAGGTGC TGGCTCAGTT CGGAGAGAGG 
GCCTCAGAGG TCTGTCTGGA TGACACAGTG ACCACAGAGA AGGAGCTGGA CACTGTGGAA 
GTGCTGAAAG CAATTCAGAA AGCCAAGGAG GTCAAGTCCA AACTGAGCAA CCCAGAGAAG 
AAGGGTGGTG AAGACTCCCG GCTCTCAGCT GCCCCCTGCA TCAGACCCAG CAGCTCCCCT 
CCCACTGTGG CTCCCGCATC TGCCTCCCTG CCCCAGCCCA TCCTCTCTAA CCAAGGAATC 
ATGTTCGTTC AGGAGGAGGC CCTGGCCAGC AGCCTCTCGT CCACTGACAG TCTGACTCCC 
GAGCACCAGC CCATTGCCCA GGGATGTTCT GATTCCTTGG AGTCCATCCC TGCGGGACAG 
GCAGCTTCCG ATGATTTAAG GGACGTGCCA GGAGCTGTTG GTGGTGCAAG CCCAGAACAT 
GCCGAGCCGG AGGTCCAGGT GGTGCCGGGG TCTGGCCAGA TCATCTTCCT GCCCTTCACC 
TGCATTGGCT ACACGGCCAC CAATCAGGAC TTCATCCAGC GCCTGAGCAC ACTGATCCGG 
CAGGCCATCG AGCGGCAGCT GCCTGCCTGG ATCGAGGCTG CCAACCAGCG GGAGGAGGGC 
CAGGGTGAAC AGGGCGAGGA GGAGGATGAG GAGGAGGAAG AAGAGGAGGA CGTGGCTGAG 
AACCGCTACT TTGAAATGGG GCCCCCAGAC GTGGAGGAGG AGGAGGGAGG AGGCCAGGGG 
GAGGAAGAGG AGGAGGAAGA GGAGGATGAA GAGGCCGAGG AGGAGCGCCT GGCTCTGGAA 
TGGGCCCTGG GCGCGGACGA GGACTTCCTG CTGGAGCACA TCCGCATCCT CAAGGTGCTG 
TGGTGCTTCC TGATCCATGT GCAGGGCAGT ATCCGCCAGT TCGCCGCCTG CCTTGTGCTC 
ACCGACTTCG GCATCGCAGT CTTCGAGATC CCGCACCAGG AGTCTCGGGG CAGCAGCCAG 
CACATCCTCT CCTCCCTGCG CTTTGTCTTT TGCTTCCCGC ATGGCGACCT CACCGAGTTT 
GGCTTCCTCA TGCCGGAGCT GTGTCTGGTG CTCAAGGTAC GGCACAGTGA GAACACGCTC 
TTCATTATCT CGGACGCCGC CAACCTGCAC GAGTTCCACG CGGACCTGCG CTCATGCTTT 
GCACCCCAGC ACATGGCCAT GCTGTGTAGC CCCATCCTCT ACGGCAGCCA CACCAGCCTG 
CAGGAGTTCC TGCGCCAGCT GCTCACCTTC TACAAGGTGG CTGGCGGCTG CCAGGAGCGC 
AGCCAGGGCT GCTTCCCCGT CTACCTGGTC TACAGTGACA AGCGCATGGT GCAGACGGCC 
GCCGGGGACT ACTCAGGCAA CATCGAGTGG GCCAGCTGCA CACTCTGTTC AGCCGTGCGG 
CGCTCCTGCT GCGCGCCCTC TGAGGCCGTC AAGTCCGCCG CCATCCCCTA CTGGCTGTTG 
CTCACGCCCC AGCACCTCAA CGTCATCAAG GCCGACTTCA ACCCCATGCC CAACCGTGGC 
ACCCACAACT GTCGCAACCG CAACAGCTTC AAGCTCAGCC GTGTGCCGCT CTCCACCGTG 
CTGCTGGACC CCACACGCAG CTGTACCCAG CCTCGGGGCG CCTTTGCTGA TGGCCACGTG 
CTAGAGCTGC TCGTGGGGTA CCGCTTTGTC ACTGCCATCT TCGTGCTGCC CCACGAGAAG 
TTCCACTTCC TGCGCGTCTA CAACCAGCTG CGGGCCTCGC TGCAGGACCT GAAGACTGTG 
GTCATCGCCA AGACCCCCGG GACGGGAGGC AGCCCCCAGG GCTCCTTTGC GGATGGCCAG 
CCTGCCGAGC GCAGGGCCAG CAATGACCAG CGTCCCCAGG AGGTCCCAGC AGAGGCTCTG 
GCCCCGGCCC CAGCGGAAGT CCCAGCTCCA GCCCCTGCAG CAGCCTCAGC CTCAGGCCCA 
GCGAAGACTC CGGCCCCAGC AGAGGCCTCA ACTTCAGCTT TGGTCCCAGA GGAGACGCCA 
GTGGAAGCTC CAGCCCCACC CCCAGCCGAG GCCCCTGCCC AGTACCCGAG TGAGCACCTC 
ATCCAGGCCA CCTCGGAGGA GAATCAGATC CCCTCGCACT TGCCTGCCTG CCCGTCGCTC 
CGGCACGTCG CCAGCCTGCG GGGCAGCGCC ATCATCGAGC TCTTCCACAG CAGCATTGCT 
GAGGTTGAAA ACGAGGAGCT GAGGCACCTC ATGTGGTCCT CGGTGGTGTT CTACCAGACC 
CCAGGGCTGG AGGTGACTGC CTGCGTGCTG CTCTCCACCA AGGCTGTGTA CTTTGTGCTC 
CACGACGGCC TCCGCCGCTA CTTCTCAGAG CCACTGCAGG ATTTCTGGCA TCAGAAAAAC 
ACCGACTACA ACAACAGCCC TTTCCACATC TCCCAGTGCT TCGTGCTAAA GCTTAGTGAC 
CTGCAGTCAG TCAATGTGGG GCTTTTCGAC CAGCATTTCC GGCTGACGGG TTCCACCCCG 
ATGCAGGTGG TCACGTGCTT GACGCGGGAC AGCTACCTGA CGCACTGCTT CCTCCAGCAC 
CTCATGGTCG TGCTGTCCTC TCTGGAACGC ACGCCCTCGC CGGAGCCTGT TGACAAGGAC 
TTCTACTCCG AGTTTGGGAA CAAGACCACA GGGAAGATGG AGAACTACGA GCTGATCCAC 
TCTAGTCGCG TCAAGTTTAC CTACCCCAGT GAGGAGGAGA TTGGGGACCT GACGTTCACT 
GTGGCCCAAA AGATGGCTGA GCCAGAGAAG GCCCCAGCCC TCAGCATCCT GCTGTACGTG 
CAGGCCTTCC AGGTGGGCAT GCCACCCCCT GGGTGCTGCA GGGGCCCCCT GCGCCCCAAG 
ACACTCCTGC TCACCAGCTC CGAGATCTTC CTCCTGGATG AGGACTGTGT CCACTACCCA 
CTGCCCGAGT TTGCCAAAGA GCCGCCGCAG AGAGACAGGT ACCGGCTGGA CGATGGCCGC 
CGCGTCCGGG ACCTGGACCG AGTGCTCATG GGCTACCAGA CCTACCCGCA GGCCCTCACC 
CTCGTCTTCG ATGACGTGCA AGGTCATGAC CTCATGGGCA GTGTCACCCT GGACCACTTT 
GGGGAGGTGC CAGGTGGCCC GGCTAGAGCC AGCCAGGGCC GTGAAGTCCA GTGGCAGGTG 
TTTGTCCCCA GTGCTGAGAG CAGAGAGAAG CTCATCTCGC TGTTGGCTCG CCAGTGGGAG 
GCCCTGTGTG GCCGTGAGCT GCCTGTCGAG CTCACCGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NISCH ( XM_006712955.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713018.1 Homo sapiens nischarin (NISCH), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu36940
Clone ID Related Accession (Same CDS sequence)	XM_006712955.2 , XM_006712955.3
Accession Version	XM_006712955.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nischarin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712955.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGTG GAATCATGTT CGTTCAGGAG GAGGCCCTGG CCAGCAGCCT CTCGTCCACT 
GACAGTCTGA CTCCCGAGCA CCAGCCCATT GCCCAGGGAT GTTCTGATTC CTTGGAGTCC 
ATCCCTGCGG GACAGGCAGC TTCCGATGAT TTAAGGGACG TGCCAGGAGC TGTTGGTGGT 
GCAAGCCCAG AACATGCCGA GCCGGAGGTC CAGGTGGTGC CGGGGTCTGG CCAGATCATC 
TTCCTGCCCT TCACCTGCAT TGGCTACACG GCCACCAATC AGGACTTCAT CCAGCGCCTG 
AGCACACTGA TCCGGCAGGC CATCGAGCGG CAGCTGCCTG CCTGGATCGA GGCTGCCAAC 
CAGCGGGAGG AGGGCCAGGG TGAACAGGGC GAGGAGGAGG ATGAGGAGGA GGAAGAAGAG 
GAGGACGTGG CTGAGAACCG CTACTTTGAA ATGGGGCCCC CAGACGTGGA GGAGGAGGAG 
GGAGGAGGCC AGGGGGAGGA AGAGGAGGAG GAAGAGGAGG ATGAAGAGGC CGAGGAGGAG 
CGCCTGGCTC TGGAATGGGC CCTGGGCGCG GACGAGGACT TCCTGCTGGA GCACATCCGC 
ATCCTCAAGG TGCTGTGGTG CTTCCTGATC CATGTGCAGG GCAGTATCCG CCAGTTCGCC 
GCCTGCCTTG TGCTCACCGA CTTCGGCATC GCAGTCTTCG AGATCCCGCA CCAGGAGTCT 
CGGGGCAGCA GCCAGCACAT CCTCTCCTCC CTGCGCTTTG TCTTTTGCTT CCCGCATGGC 
GACCTCACCG AGTTTGGCTT CCTCATGCCG GAGCTGTGTC TGGTGCTCAA GGTACGGCAC 
AGTGAGAACA CGCTCTTCAT TATCTCGGAC GCCGCCAACC TGCACGAGTT CCACGCGGAC 
CTGCGCTCAT GCTTTGCACC CCAGCACATG GCCATGCTGT GTAGCCCCAT CCTCTACGGC 
AGCCACACCA GCCTGCAGGA GTTCCTGCGC CAGCTGCTCA CCTTCTACAA GGTGGCTGGC 
GGCTGCCAGG AGCGCAGCCA GGGCTGCTTC CCCGTCTACC TGGTCTACAG TGACAAGCGC 
ATGGTGCAGA CGGCCGCCGG GGACTACTCA GGCAACATCG AGTGGGCCAG CTGCACACTC 
TGTTCAGCCG TGCGGCGCTC CTGCTGCGCG CCCTCTGAGG CCGTCAAGTC CGCCGCCATC 
CCCTACTGGC TGTTGCTCAC GCCCCAGCAC CTCAACGTCA TCAAGGCCGA CTTCAACCCC 
ATGCCCAACC GTGGCACCCA CAACTGTCGC AACCGCAACA GCTTCAAGCT CAGCCGTGTG 
CCGCTCTCCA CCGTGCTGCT GGACCCCACA CGCAGCTGTA CCCAGCCTCG GGGCGCCTTT 
GCTGATGGCC ACGTGCTAGA GCTGCTCGTG GGGTACCGCT TTGTCACTGC CATCTTCGTG 
CTGCCCCACG AGAAGTTCCA CTTCCTGCGC GTCTACAACC AGCTGCGGGC CTCGCTGCAG 
GACCTGAAGA CTGTGGTCAT CGCCAAGACC CCCGGGACGG GAGGCAGCCC CCAGGGCTCC 
TTTGCGGATG GCCAGCCTGC CGAGCGCAGG GCCAGCAATG ACCAGCGTCC CCAGGAGGTC 
CCAGCAGAGG CTCTGGCCCC GGCCCCAGCG GAAGTCCCAG CTCCAGCCCC TGCAGCAGCC 
TCAGCCTCAG GCCCAGCGAA GACTCCGGCC CCAGCAGAGG CCTCAACTTC AGCTTTGGTC 
CCAGAGGAGA CGCCAGTGGA AGCTCCAGCC CCACCCCCAG CCGAGGCCCC TGCCCAGTAC 
CCGAGTGAGC ACCTCATCCA GGCCACCTCG GAGGAGAATC AGATCCCCTC GCACTTGCCT 
GCCTGCCCGT CGCTCCGGCA CGTCGCCAGC CTGCGGGGCA GCGCCATCAT CGAGCTCTTC 
CACAGCAGCA TTGCTGAGGT TGAAAACGAG GAGCTGAGGC ACCTCATGTG GTCCTCGGTG 
GTGTTCTACC AGACCCCAGG GCTGGAGGTG ACTGCCTGCG TGCTGCTCTC CACCAAGGCT 
GTGTACTTTG TGCTCCACGA CGGCCTCCGC CGCTACTTCT CAGAGCCACT GCAGGATTTC 
TGGCATCAGA AAAACACCGA CTACAACAAC AGCCCTTTCC ACATCTCCCA GTGCTTCGTG 
CTAAAGCTTA GTGACCTGCA GTCAGTCAAT GTGGGGCTTT TCGACCAGCA TTTCCGGCTG 
ACGGGTTCCA CCCCGATGCA GGTGGTCACG TGCTTGACGC GGGACAGCTA CCTGACGCAC 
TGCTTCCTCC AGCACCTCAT GGTCGTGCTG TCCTCTCTGG AACGCACGCC CTCGCCGGAG 
CCTGTTGACA AGGACTTCTA CTCCGAGTTT GGGAACAAGA CCACAGGGAA GATGGAGAAC 
TACGAGCTGA TCCACTCTAG TCGCGTCAAG TTTACCTACC CCAGTGAGGA GGAGATTGGG 
GACCTGACGT TCACTGTGGC CCAAAAGATG GCTGAGCCAG AGAAGGCCCC AGCCCTCAGC 
ATCCTGCTGT ACGTGCAGGC CTTCCAGGTG GGCATGCCAC CCCCTGGGTG CTGCAGGGGC 
CCCCTGCGCC CCAAGACACT CCTGCTCACC AGCTCCGAGA TCTTCCTCCT GGATGAGGAC 
TGTGTCCACT ACCCACTGCC CGAGTTTGCC AAAGAGCCGC CGCAGAGAGA CAGGTACCGG 
CTGGACGATG GCCGCCGCGT CCGGGACCTG GACCGAGTGC TCATGGGCTA CCAGACCTAC 
CCGCAGGCCC TCACCCTCGT CTTCGATGAC GTGCAAGGTC ATGACCTCAT GGGCAGTGTC 
ACCCTGGACC ACTTTGGGGA GGTGCCAGGT GGCCCGGCTA GAGCCAGCCA GGGCCGTGAA 
GTCCAGTGGC AGGTGTTTGT CCCCAGTGCT GAGAGCAGAG AGAAGCTCAT CTCGCTGTTG 
GCTCGCCAGT GGGAGGCCCT GTGTGGCCGT GAGCTGCCTG TCGAGCTCAC CGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713018.1
CDS	2180..5176
Translation	MKCGIMFVQEEALASSLSSTDSLTPEHQPIAQGCSDSLESIPAGQAASDDLRDVPGAVGGASPEHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREEGQGEQGEEEDEEEEEEEDVAENRYFEMGPPDVEEEEGGGQGEEEEEEEEDEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTSLQEFLRQLLTFYKVAGGCQERSQGCFPVYLVYSDKRMVQTAAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTPQHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVIAKTPGTGGSPQGSFADGQPAERRASNDQRPQEVPAEALAPAPAEVPAPAPAAASASGPAKTPAPAEASTSALVPEETPVEAPAPPPAEAPAQYPSEHLIQATSEENQIPSHLPACPSLRHVASLRGSAIIELFHSSIAEVENEELRHLMWSSVVFYQTPGLEVTACVLLSTKAVYFVLHDGLRRYFSEPLQDFWHQKNTDYNNSPFHISQCFVLKLSDLQSVNVGLFDQHFRLTGSTPMQVVTCLTRDSYLTHCFLQHLMVVLSSLERTPSPEPVDKDFYSEFGNKTTGKMENYELIHSSRVKFTYPSEEEIGDLTFTVAQKMAEPEKAPALSILLYVQAFQVGMPPPGCCRGPLRPKTLLLTSSEIFLLDEDCVHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYQTYPQALTLVFDDVQGHDLMGSVTLDHFGEVPGGPARASQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

Target ORF information:

RefSeq Version	XM_006712955.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nischarin (NISCH), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712955.2

ORF Insert Sequence:

ATGAAATGTG GAATCATGTT CGTTCAGGAG GAGGCCCTGG CCAGCAGCCT CTCGTCCACT 
GACAGTCTGA CTCCCGAGCA CCAGCCCATT GCCCAGGGAT GTTCTGATTC CTTGGAGTCC 
ATCCCTGCGG GACAGGCAGC TTCCGATGAT TTAAGGGACG TGCCAGGAGC TGTTGGTGGT 
GCAAGCCCAG AACATGCCGA GCCGGAGGTC CAGGTGGTGC CGGGGTCTGG CCAGATCATC 
TTCCTGCCCT TCACCTGCAT TGGCTACACG GCCACCAATC AGGACTTCAT CCAGCGCCTG 
AGCACACTGA TCCGGCAGGC CATCGAGCGG CAGCTGCCTG CCTGGATCGA GGCTGCCAAC 
CAGCGGGAGG AGGGCCAGGG TGAACAGGGC GAGGAGGAGG ATGAGGAGGA GGAAGAAGAG 
GAGGACGTGG CTGAGAACCG CTACTTTGAA ATGGGGCCCC CAGACGTGGA GGAGGAGGAG 
GGAGGAGGCC AGGGGGAGGA AGAGGAGGAG GAAGAGGAGG ATGAAGAGGC CGAGGAGGAG 
CGCCTGGCTC TGGAATGGGC CCTGGGCGCG GACGAGGACT TCCTGCTGGA GCACATCCGC 
ATCCTCAAGG TGCTGTGGTG CTTCCTGATC CATGTGCAGG GCAGTATCCG CCAGTTCGCC 
GCCTGCCTTG TGCTCACCGA CTTCGGCATC GCAGTCTTCG AGATCCCGCA CCAGGAGTCT 
CGGGGCAGCA GCCAGCACAT CCTCTCCTCC CTGCGCTTTG TCTTTTGCTT CCCGCATGGC 
GACCTCACCG AGTTTGGCTT CCTCATGCCG GAGCTGTGTC TGGTGCTCAA GGTACGGCAC 
AGTGAGAACA CGCTCTTCAT TATCTCGGAC GCCGCCAACC TGCACGAGTT CCACGCGGAC 
CTGCGCTCAT GCTTTGCACC CCAGCACATG GCCATGCTGT GTAGCCCCAT CCTCTACGGC 
AGCCACACCA GCCTGCAGGA GTTCCTGCGC CAGCTGCTCA CCTTCTACAA GGTGGCTGGC 
GGCTGCCAGG AGCGCAGCCA GGGCTGCTTC CCCGTCTACC TGGTCTACAG TGACAAGCGC 
ATGGTGCAGA CGGCCGCCGG GGACTACTCA GGCAACATCG AGTGGGCCAG CTGCACACTC 
TGTTCAGCCG TGCGGCGCTC CTGCTGCGCG CCCTCTGAGG CCGTCAAGTC CGCCGCCATC 
CCCTACTGGC TGTTGCTCAC GCCCCAGCAC CTCAACGTCA TCAAGGCCGA CTTCAACCCC 
ATGCCCAACC GTGGCACCCA CAACTGTCGC AACCGCAACA GCTTCAAGCT CAGCCGTGTG 
CCGCTCTCCA CCGTGCTGCT GGACCCCACA CGCAGCTGTA CCCAGCCTCG GGGCGCCTTT 
GCTGATGGCC ACGTGCTAGA GCTGCTCGTG GGGTACCGCT TTGTCACTGC CATCTTCGTG 
CTGCCCCACG AGAAGTTCCA CTTCCTGCGC GTCTACAACC AGCTGCGGGC CTCGCTGCAG 
GACCTGAAGA CTGTGGTCAT CGCCAAGACC CCCGGGACGG GAGGCAGCCC CCAGGGCTCC 
TTTGCGGATG GCCAGCCTGC CGAGCGCAGG GCCAGCAATG ACCAGCGTCC CCAGGAGGTC 
CCAGCAGAGG CTCTGGCCCC GGCCCCAGCG GAAGTCCCAG CTCCAGCCCC TGCAGCAGCC 
TCAGCCTCAG GCCCAGCGAA GACTCCGGCC CCAGCAGAGG CCTCAACTTC AGCTTTGGTC 
CCAGAGGAGA CGCCAGTGGA AGCTCCAGCC CCACCCCCAG CCGAGGCCCC TGCCCAGTAC 
CCGAGTGAGC ACCTCATCCA GGCCACCTCG GAGGAGAATC AGATCCCCTC GCACTTGCCT 
GCCTGCCCGT CGCTCCGGCA CGTCGCCAGC CTGCGGGGCA GCGCCATCAT CGAGCTCTTC 
CACAGCAGCA TTGCTGAGGT TGAAAACGAG GAGCTGAGGC ACCTCATGTG GTCCTCGGTG 
GTGTTCTACC AGACCCCAGG GCTGGAGGTG ACTGCCTGCG TGCTGCTCTC CACCAAGGCT 
GTGTACTTTG TGCTCCACGA CGGCCTCCGC CGCTACTTCT CAGAGCCACT GCAGGATTTC 
TGGCATCAGA AAAACACCGA CTACAACAAC AGCCCTTTCC ACATCTCCCA GTGCTTCGTG 
CTAAAGCTTA GTGACCTGCA GTCAGTCAAT GTGGGGCTTT TCGACCAGCA TTTCCGGCTG 
ACGGGTTCCA CCCCGATGCA GGTGGTCACG TGCTTGACGC GGGACAGCTA CCTGACGCAC 
TGCTTCCTCC AGCACCTCAT GGTCGTGCTG TCCTCTCTGG AACGCACGCC CTCGCCGGAG 
CCTGTTGACA AGGACTTCTA CTCCGAGTTT GGGAACAAGA CCACAGGGAA GATGGAGAAC 
TACGAGCTGA TCCACTCTAG TCGCGTCAAG TTTACCTACC CCAGTGAGGA GGAGATTGGG 
GACCTGACGT TCACTGTGGC CCAAAAGATG GCTGAGCCAG AGAAGGCCCC AGCCCTCAGC 
ATCCTGCTGT ACGTGCAGGC CTTCCAGGTG GGCATGCCAC CCCCTGGGTG CTGCAGGGGC 
CCCCTGCGCC CCAAGACACT CCTGCTCACC AGCTCCGAGA TCTTCCTCCT GGATGAGGAC 
TGTGTCCACT ACCCACTGCC CGAGTTTGCC AAAGAGCCGC CGCAGAGAGA CAGGTACCGG 
CTGGACGATG GCCGCCGCGT CCGGGACCTG GACCGAGTGC TCATGGGCTA CCAGACCTAC 
CCGCAGGCCC TCACCCTCGT CTTCGATGAC GTGCAAGGTC ATGACCTCAT GGGCAGTGTC 
ACCCTGGACC ACTTTGGGGA GGTGCCAGGT GGCCCGGCTA GAGCCAGCCA GGGCCGTGAA 
GTCCAGTGGC AGGTGTTTGT CCCCAGTGCT GAGAGCAGAG AGAAGCTCAT CTCGCTGTTG 
GCTCGCCAGT GGGAGGCCCT GTGTGGCCGT GAGCTGCCTG TCGAGCTCAC CGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NISCH ( XM_006712955.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713018.1 Homo sapiens nischarin (NISCH), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu36940
Clone ID Related Accession (Same CDS sequence)	XM_006712955.2 , XM_006712955.3
Accession Version	XM_006712955.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nischarin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006712955.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGTG GAATCATGTT CGTTCAGGAG GAGGCCCTGG CCAGCAGCCT CTCGTCCACT 
GACAGTCTGA CTCCCGAGCA CCAGCCCATT GCCCAGGGAT GTTCTGATTC CTTGGAGTCC 
ATCCCTGCGG GACAGGCAGC TTCCGATGAT TTAAGGGACG TGCCAGGAGC TGTTGGTGGT 
GCAAGCCCAG AACATGCCGA GCCGGAGGTC CAGGTGGTGC CGGGGTCTGG CCAGATCATC 
TTCCTGCCCT TCACCTGCAT TGGCTACACG GCCACCAATC AGGACTTCAT CCAGCGCCTG 
AGCACACTGA TCCGGCAGGC CATCGAGCGG CAGCTGCCTG CCTGGATCGA GGCTGCCAAC 
CAGCGGGAGG AGGGCCAGGG TGAACAGGGC GAGGAGGAGG ATGAGGAGGA GGAAGAAGAG 
GAGGACGTGG CTGAGAACCG CTACTTTGAA ATGGGGCCCC CAGACGTGGA GGAGGAGGAG 
GGAGGAGGCC AGGGGGAGGA AGAGGAGGAG GAAGAGGAGG ATGAAGAGGC CGAGGAGGAG 
CGCCTGGCTC TGGAATGGGC CCTGGGCGCG GACGAGGACT TCCTGCTGGA GCACATCCGC 
ATCCTCAAGG TGCTGTGGTG CTTCCTGATC CATGTGCAGG GCAGTATCCG CCAGTTCGCC 
GCCTGCCTTG TGCTCACCGA CTTCGGCATC GCAGTCTTCG AGATCCCGCA CCAGGAGTCT 
CGGGGCAGCA GCCAGCACAT CCTCTCCTCC CTGCGCTTTG TCTTTTGCTT CCCGCATGGC 
GACCTCACCG AGTTTGGCTT CCTCATGCCG GAGCTGTGTC TGGTGCTCAA GGTACGGCAC 
AGTGAGAACA CGCTCTTCAT TATCTCGGAC GCCGCCAACC TGCACGAGTT CCACGCGGAC 
CTGCGCTCAT GCTTTGCACC CCAGCACATG GCCATGCTGT GTAGCCCCAT CCTCTACGGC 
AGCCACACCA GCCTGCAGGA GTTCCTGCGC CAGCTGCTCA CCTTCTACAA GGTGGCTGGC 
GGCTGCCAGG AGCGCAGCCA GGGCTGCTTC CCCGTCTACC TGGTCTACAG TGACAAGCGC 
ATGGTGCAGA CGGCCGCCGG GGACTACTCA GGCAACATCG AGTGGGCCAG CTGCACACTC 
TGTTCAGCCG TGCGGCGCTC CTGCTGCGCG CCCTCTGAGG CCGTCAAGTC CGCCGCCATC 
CCCTACTGGC TGTTGCTCAC GCCCCAGCAC CTCAACGTCA TCAAGGCCGA CTTCAACCCC 
ATGCCCAACC GTGGCACCCA CAACTGTCGC AACCGCAACA GCTTCAAGCT CAGCCGTGTG 
CCGCTCTCCA CCGTGCTGCT GGACCCCACA CGCAGCTGTA CCCAGCCTCG GGGCGCCTTT 
GCTGATGGCC ACGTGCTAGA GCTGCTCGTG GGGTACCGCT TTGTCACTGC CATCTTCGTG 
CTGCCCCACG AGAAGTTCCA CTTCCTGCGC GTCTACAACC AGCTGCGGGC CTCGCTGCAG 
GACCTGAAGA CTGTGGTCAT CGCCAAGACC CCCGGGACGG GAGGCAGCCC CCAGGGCTCC 
TTTGCGGATG GCCAGCCTGC CGAGCGCAGG GCCAGCAATG ACCAGCGTCC CCAGGAGGTC 
CCAGCAGAGG CTCTGGCCCC GGCCCCAGCG GAAGTCCCAG CTCCAGCCCC TGCAGCAGCC 
TCAGCCTCAG GCCCAGCGAA GACTCCGGCC CCAGCAGAGG CCTCAACTTC AGCTTTGGTC 
CCAGAGGAGA CGCCAGTGGA AGCTCCAGCC CCACCCCCAG CCGAGGCCCC TGCCCAGTAC 
CCGAGTGAGC ACCTCATCCA GGCCACCTCG GAGGAGAATC AGATCCCCTC GCACTTGCCT 
GCCTGCCCGT CGCTCCGGCA CGTCGCCAGC CTGCGGGGCA GCGCCATCAT CGAGCTCTTC 
CACAGCAGCA TTGCTGAGGT TGAAAACGAG GAGCTGAGGC ACCTCATGTG GTCCTCGGTG 
GTGTTCTACC AGACCCCAGG GCTGGAGGTG ACTGCCTGCG TGCTGCTCTC CACCAAGGCT 
GTGTACTTTG TGCTCCACGA CGGCCTCCGC CGCTACTTCT CAGAGCCACT GCAGGATTTC 
TGGCATCAGA AAAACACCGA CTACAACAAC AGCCCTTTCC ACATCTCCCA GTGCTTCGTG 
CTAAAGCTTA GTGACCTGCA GTCAGTCAAT GTGGGGCTTT TCGACCAGCA TTTCCGGCTG 
ACGGGTTCCA CCCCGATGCA GGTGGTCACG TGCTTGACGC GGGACAGCTA CCTGACGCAC 
TGCTTCCTCC AGCACCTCAT GGTCGTGCTG TCCTCTCTGG AACGCACGCC CTCGCCGGAG 
CCTGTTGACA AGGACTTCTA CTCCGAGTTT GGGAACAAGA CCACAGGGAA GATGGAGAAC 
TACGAGCTGA TCCACTCTAG TCGCGTCAAG TTTACCTACC CCAGTGAGGA GGAGATTGGG 
GACCTGACGT TCACTGTGGC CCAAAAGATG GCTGAGCCAG AGAAGGCCCC AGCCCTCAGC 
ATCCTGCTGT ACGTGCAGGC CTTCCAGGTG GGCATGCCAC CCCCTGGGTG CTGCAGGGGC 
CCCCTGCGCC CCAAGACACT CCTGCTCACC AGCTCCGAGA TCTTCCTCCT GGATGAGGAC 
TGTGTCCACT ACCCACTGCC CGAGTTTGCC AAAGAGCCGC CGCAGAGAGA CAGGTACCGG 
CTGGACGATG GCCGCCGCGT CCGGGACCTG GACCGAGTGC TCATGGGCTA CCAGACCTAC 
CCGCAGGCCC TCACCCTCGT CTTCGATGAC GTGCAAGGTC ATGACCTCAT GGGCAGTGTC 
ACCCTGGACC ACTTTGGGGA GGTGCCAGGT GGCCCGGCTA GAGCCAGCCA GGGCCGTGAA 
GTCCAGTGGC AGGTGTTTGT CCCCAGTGCT GAGAGCAGAG AGAAGCTCAT CTCGCTGTTG 
GCTCGCCAGT GGGAGGCCCT GTGTGGCCGT GAGCTGCCTG TCGAGCTCAC CGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713018.1
CDS	1911..4907
Translation	MKCGIMFVQEEALASSLSSTDSLTPEHQPIAQGCSDSLESIPAGQAASDDLRDVPGAVGGASPEHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREEGQGEQGEEEDEEEEEEEDVAENRYFEMGPPDVEEEEGGGQGEEEEEEEEDEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTSLQEFLRQLLTFYKVAGGCQERSQGCFPVYLVYSDKRMVQTAAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTPQHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVIAKTPGTGGSPQGSFADGQPAERRASNDQRPQEVPAEALAPAPAEVPAPAPAAASASGPAKTPAPAEASTSALVPEETPVEAPAPPPAEAPAQYPSEHLIQATSEENQIPSHLPACPSLRHVASLRGSAIIELFHSSIAEVENEELRHLMWSSVVFYQTPGLEVTACVLLSTKAVYFVLHDGLRRYFSEPLQDFWHQKNTDYNNSPFHISQCFVLKLSDLQSVNVGLFDQHFRLTGSTPMQVVTCLTRDSYLTHCFLQHLMVVLSSLERTPSPEPVDKDFYSEFGNKTTGKMENYELIHSSRVKFTYPSEEEIGDLTFTVAQKMAEPEKAPALSILLYVQAFQVGMPPPGCCRGPLRPKTLLLTSSEIFLLDEDCVHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYQTYPQALTLVFDDVQGHDLMGSVTLDHFGEVPGGPARASQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

Target ORF information:

RefSeq Version	XM_006712955.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nischarin (NISCH), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712955.3

ORF Insert Sequence:

ATGAAATGTG GAATCATGTT CGTTCAGGAG GAGGCCCTGG CCAGCAGCCT CTCGTCCACT 
GACAGTCTGA CTCCCGAGCA CCAGCCCATT GCCCAGGGAT GTTCTGATTC CTTGGAGTCC 
ATCCCTGCGG GACAGGCAGC TTCCGATGAT TTAAGGGACG TGCCAGGAGC TGTTGGTGGT 
GCAAGCCCAG AACATGCCGA GCCGGAGGTC CAGGTGGTGC CGGGGTCTGG CCAGATCATC 
TTCCTGCCCT TCACCTGCAT TGGCTACACG GCCACCAATC AGGACTTCAT CCAGCGCCTG 
AGCACACTGA TCCGGCAGGC CATCGAGCGG CAGCTGCCTG CCTGGATCGA GGCTGCCAAC 
CAGCGGGAGG AGGGCCAGGG TGAACAGGGC GAGGAGGAGG ATGAGGAGGA GGAAGAAGAG 
GAGGACGTGG CTGAGAACCG CTACTTTGAA ATGGGGCCCC CAGACGTGGA GGAGGAGGAG 
GGAGGAGGCC AGGGGGAGGA AGAGGAGGAG GAAGAGGAGG ATGAAGAGGC CGAGGAGGAG 
CGCCTGGCTC TGGAATGGGC CCTGGGCGCG GACGAGGACT TCCTGCTGGA GCACATCCGC 
ATCCTCAAGG TGCTGTGGTG CTTCCTGATC CATGTGCAGG GCAGTATCCG CCAGTTCGCC 
GCCTGCCTTG TGCTCACCGA CTTCGGCATC GCAGTCTTCG AGATCCCGCA CCAGGAGTCT 
CGGGGCAGCA GCCAGCACAT CCTCTCCTCC CTGCGCTTTG TCTTTTGCTT CCCGCATGGC 
GACCTCACCG AGTTTGGCTT CCTCATGCCG GAGCTGTGTC TGGTGCTCAA GGTACGGCAC 
AGTGAGAACA CGCTCTTCAT TATCTCGGAC GCCGCCAACC TGCACGAGTT CCACGCGGAC 
CTGCGCTCAT GCTTTGCACC CCAGCACATG GCCATGCTGT GTAGCCCCAT CCTCTACGGC 
AGCCACACCA GCCTGCAGGA GTTCCTGCGC CAGCTGCTCA CCTTCTACAA GGTGGCTGGC 
GGCTGCCAGG AGCGCAGCCA GGGCTGCTTC CCCGTCTACC TGGTCTACAG TGACAAGCGC 
ATGGTGCAGA CGGCCGCCGG GGACTACTCA GGCAACATCG AGTGGGCCAG CTGCACACTC 
TGTTCAGCCG TGCGGCGCTC CTGCTGCGCG CCCTCTGAGG CCGTCAAGTC CGCCGCCATC 
CCCTACTGGC TGTTGCTCAC GCCCCAGCAC CTCAACGTCA TCAAGGCCGA CTTCAACCCC 
ATGCCCAACC GTGGCACCCA CAACTGTCGC AACCGCAACA GCTTCAAGCT CAGCCGTGTG 
CCGCTCTCCA CCGTGCTGCT GGACCCCACA CGCAGCTGTA CCCAGCCTCG GGGCGCCTTT 
GCTGATGGCC ACGTGCTAGA GCTGCTCGTG GGGTACCGCT TTGTCACTGC CATCTTCGTG 
CTGCCCCACG AGAAGTTCCA CTTCCTGCGC GTCTACAACC AGCTGCGGGC CTCGCTGCAG 
GACCTGAAGA CTGTGGTCAT CGCCAAGACC CCCGGGACGG GAGGCAGCCC CCAGGGCTCC 
TTTGCGGATG GCCAGCCTGC CGAGCGCAGG GCCAGCAATG ACCAGCGTCC CCAGGAGGTC 
CCAGCAGAGG CTCTGGCCCC GGCCCCAGCG GAAGTCCCAG CTCCAGCCCC TGCAGCAGCC 
TCAGCCTCAG GCCCAGCGAA GACTCCGGCC CCAGCAGAGG CCTCAACTTC AGCTTTGGTC 
CCAGAGGAGA CGCCAGTGGA AGCTCCAGCC CCACCCCCAG CCGAGGCCCC TGCCCAGTAC 
CCGAGTGAGC ACCTCATCCA GGCCACCTCG GAGGAGAATC AGATCCCCTC GCACTTGCCT 
GCCTGCCCGT CGCTCCGGCA CGTCGCCAGC CTGCGGGGCA GCGCCATCAT CGAGCTCTTC 
CACAGCAGCA TTGCTGAGGT TGAAAACGAG GAGCTGAGGC ACCTCATGTG GTCCTCGGTG 
GTGTTCTACC AGACCCCAGG GCTGGAGGTG ACTGCCTGCG TGCTGCTCTC CACCAAGGCT 
GTGTACTTTG TGCTCCACGA CGGCCTCCGC CGCTACTTCT CAGAGCCACT GCAGGATTTC 
TGGCATCAGA AAAACACCGA CTACAACAAC AGCCCTTTCC ACATCTCCCA GTGCTTCGTG 
CTAAAGCTTA GTGACCTGCA GTCAGTCAAT GTGGGGCTTT TCGACCAGCA TTTCCGGCTG 
ACGGGTTCCA CCCCGATGCA GGTGGTCACG TGCTTGACGC GGGACAGCTA CCTGACGCAC 
TGCTTCCTCC AGCACCTCAT GGTCGTGCTG TCCTCTCTGG AACGCACGCC CTCGCCGGAG 
CCTGTTGACA AGGACTTCTA CTCCGAGTTT GGGAACAAGA CCACAGGGAA GATGGAGAAC 
TACGAGCTGA TCCACTCTAG TCGCGTCAAG TTTACCTACC CCAGTGAGGA GGAGATTGGG 
GACCTGACGT TCACTGTGGC CCAAAAGATG GCTGAGCCAG AGAAGGCCCC AGCCCTCAGC 
ATCCTGCTGT ACGTGCAGGC CTTCCAGGTG GGCATGCCAC CCCCTGGGTG CTGCAGGGGC 
CCCCTGCGCC CCAAGACACT CCTGCTCACC AGCTCCGAGA TCTTCCTCCT GGATGAGGAC 
TGTGTCCACT ACCCACTGCC CGAGTTTGCC AAAGAGCCGC CGCAGAGAGA CAGGTACCGG 
CTGGACGATG GCCGCCGCGT CCGGGACCTG GACCGAGTGC TCATGGGCTA CCAGACCTAC 
CCGCAGGCCC TCACCCTCGT CTTCGATGAC GTGCAAGGTC ATGACCTCAT GGGCAGTGTC 
ACCCTGGACC ACTTTGGGGA GGTGCCAGGT GGCCCGGCTA GAGCCAGCCA GGGCCGTGAA 
GTCCAGTGGC AGGTGTTTGT CCCCAGTGCT GAGAGCAGAG AGAAGCTCAT CTCGCTGTTG 
GCTCGCCAGT GGGAGGCCCT GTGTGGCCGT GAGCTGCCTG TCGAGCTCAC CGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NISCH
Entrez Gene ID	11188
Full Name	nischarin
Synonyms	I-1,IR1,IRAS,hIRAS
General protein information	Preferred Names nischarin Names nischarin I-1 receptor candidate protein I1R candidate protein imidazoline receptor 1 imidazoline receptor antisera selected
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p21.1
Summary	This gene encodes a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the cell membrane to endosomes. Expression of this protein was reduced in human breast cancers while its overexpression reduced tumor growth and metastasis; possibly by limiting the expression of alpha-5 integrin. In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth and differentiation. Alternative splicing results in multiple transcript variants encoding differerent isoforms. Some isoforms lack the expected C-terminal domains of a functional imidazoline receptor. [provided by RefSeq, Jan 2013].
Disorder MIM:	615507

	NISCH	XP_001085527.2
Rattus norvegicus (Norway rat)	Nisch	XP_001057307.4
Pan troglodytes (chimpanzee)	NISCH	XP_001152331.2
Bos taurus (cattle)	NISCH	XP_002697110.2
Homo sapiens (human)	NISCH	NP_009115.2
Drosophila melanogaster (fruit fly)	CG11807	NP_001260990.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011394	XP_317926.4
Canis lupus familiaris (dog)	NISCH	XP_005633244.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NISCH cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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