NISCH cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NISCH
Entrez Gene ID	100336473
Full Name	nischarin
General protein information	Preferred Names nischarin Names nischarin LOW QUALITY PROTEIN: nischarin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024983242.1	XP_024839010.1	nischarin isoform X2
XM_015468772.1	XP_015324258.1	LOW QUALITY PROTEIN: nischarin isoform X4
XM_002697064.4	XP_002697110.4	nischarin isoform X1
XM_024983241.1	XP_024839009.1	nischarin isoform X1
XM_015468771.1	XP_015324257.1	nischarin isoform X3
XM_005222995.3	XP_005223052.2	nischarin isoform X2

Pathways from BioSystems

Homologies

	NISCH	XP_001085527.2
Rattus norvegicus (Norway rat)	Nisch	XP_001057307.4
Pan troglodytes (chimpanzee)	NISCH	XP_001152331.2
Bos taurus (cattle)	NISCH	XP_002697110.2
Homo sapiens (human)	NISCH	NP_009115.2
Drosophila melanogaster (fruit fly)	CG11807	NP_001260990.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011394	XP_317926.4
Canis lupus familiaris (dog)	NISCH	XP_005633244.1

Gene Ontology

The following NISCH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NISCH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa59890	XM_005222995.3 Latest version!	Bos taurus nischarin (NISCH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa229292	XM_024983242.1 Latest version!	Bos taurus nischarin (NISCH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa95139	XM_015468772.1 Latest version!	Bos taurus nischarin (NISCH), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OBa229291	XM_024983241.1 Latest version!	Bos taurus nischarin (NISCH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa94455	XM_002697064.4 Latest version!	Bos taurus nischarin (NISCH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa95138	XM_015468771.1 Latest version!	Bos taurus nischarin (NISCH), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NISCH ( XM_005222995.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223052.2 Bos taurus nischarin (NISCH), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa59890
Clone ID Related Accession (Same CDS sequence)	XM_005222995.3
Accession Version	XM_005222995.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nischarin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222995.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG CTAAAAGGGG CCCCCTGCTT CTCAGCAAGG GTCAGGTTTA CATCATCCAG 
ATCACTGATG GGAGCCATGA GTGGACAGTC AAGCACCGCT ACAGTGATTT CCACGACCTA 
CATGAAAAGC TTGTTGCAGA GAAGAAGATT GATAGAAGTC TGCTTCCGCC CAAAAAGATA 
ATTGGGAAAA ACTCCAGGAG CTTGGTGGAA AAGAGGGAGA AGGATCTAGA AATTTATCTC 
CAGACGCTCC TGGCCACCTT CCCTGATGTG GCCCCCAGAG TGCTGGCCCA GTTCTTGCAT 
TTCCACTTTT ACGAGATTAA TGGCATCACT GCAGCACTGG CTGAGGAACT CTTCGAAAAA 
GGAGAACAGC TCCTGGGGGC TGGCGAGGTC TTCGCCATTG GCCCCCTGCA GCTCTACGCA 
GTCACCGAGC AGCTGCAGCA GGGAAAGCCC ACGTGCGCCA GCGGGGATGC CAAGACTGAC 
CTGGGGCACA TCCTGGACTT CACCTGTCGC CTTAAGTACC TTAAGGTTTC TGGCACAGAA 
GGACCTTTTG GGACCAGCAA CATTCAGGAG CAGCTTCTGC CTTTTGATCT GTCTATATTC 
AAGTCTCTTC ATCAGGTGGA GATAAGTCAC TGTGATGCCA GGCGCATCCG GGGGCTGGTT 
GCATCCAAGC CCACCTTGGC CACAATGAGC GTCCGTTTCT CAGCAACCTC CATGAAGGAA 
GTCCTCGTTC CCGAAGCCTC GGAGTTCGAT GAGTGGGAGC CAGCAGGCGC AGCCCTGGAG 
GGCCCCGTGA CTGCCGTCAT CCCCACATGG CAGGCTCTGA CCGCTCTGGA CTTGAGTCAC 
AATAGCATCT CTGAGATCGA TGATTCTGTG AAACTGATTC CAAAGATTGA GTTCCTGGAC 
CTGAGTCACA ATGGAGTGCT GGTCATGAAC AATTTGCAGC ACCTGTACAA CCTTGTGCAC 
GTGGACCTGT CGTACAACAA GCTCTCCTCC TTGGAAGGGG CTCACACCAA ACTGGGCAAC 
ATCAAGACCC TGAACCTGGC GGGCAACCTC CTGAGGCATC TGAGTGGCCT GCATAAGCTC 
TATTCCCTGG TCAACCTGGA CCTCAGCGAC AACAGAATCG AACAGATGGA AGAGGTCAGA 
AGCATCGGCA GCCTCCCATG TCTGGAGCAC GTGGCTCTGC TGAACAACCC CCTGAGTATC 
ATCCCCGACT ATCGGACCAA GGTGCTGGCT CAGTTCGGAG AGCGGGCCTC AGAGGTCTGC 
CTGGACAACA CAGTGACCAC AGAGAAAGAG TTGGACACAG TGGAAGTGCT AAAAGCAATT 
CAGAAAGCCA AAGAGGTCAA GTCCAAATTA AGCAACCCTG AGAAGAAGGT CAGTGAGGAT 
TCTCGGCTCT CTGCTGCCCC CTGTGTCAGA CCCAGCAGCT CCCCACCCAG CGCGGCTCCC 
ACCTCTGCCT CCCTGCCCCA GCCCATCCTC TCCAACCAAG GCATCCTCGG AGATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223052.2
CDS	16..1515
Translation	MEEAKRGPLLLSKGQVYIIQITDGSHEWTVKHRYSDFHDLHEKLVAEKKIDRSLLPPKKIIGKNSRSLVEKREKDLEIYLQTLLATFPDVAPRVLAQFLHFHFYEINGITAALAEELFEKGEQLLGAGEVFAIGPLQLYAVTEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIQEQLLPFDLSIFKSLHQVEISHCDARRIRGLVASKPTLATMSVRFSATSMKEVLVPEASEFDEWEPAGAALEGPVTAVIPTWQALTALDLSHNSISEIDDSVKLIPKIEFLDLSHNGVLVMNNLQHLYNLVHVDLSYNKLSSLEGAHTKLGNIKTLNLAGNLLRHLSGLHKLYSLVNLDLSDNRIEQMEEVRSIGSLPCLEHVALLNNPLSIIPDYRTKVLAQFGERASEVCLDNTVTTEKELDTVEVLKAIQKAKEVKSKLSNPEKKVSEDSRLSAAPCVRPSSSPPSAAPTSASLPQPILSNQGILGDE

Target ORF information:

RefSeq Version	XM_005222995.3
Organism	Bos taurus(cattle)
Definition	Bos taurus nischarin (NISCH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222995.3

ORF Insert Sequence:

ATGGAGGAGG CTAAAAGGGG CCCCCTGCTT CTCAGCAAGG GTCAGGTTTA CATCATCCAG 
ATCACTGATG GGAGCCATGA GTGGACAGTC AAGCACCGCT ACAGTGATTT CCACGACCTA 
CATGAAAAGC TTGTTGCAGA GAAGAAGATT GATAGAAGTC TGCTTCCGCC CAAAAAGATA 
ATTGGGAAAA ACTCCAGGAG CTTGGTGGAA AAGAGGGAGA AGGATCTAGA AATTTATCTC 
CAGACGCTCC TGGCCACCTT CCCTGATGTG GCCCCCAGAG TGCTGGCCCA GTTCTTGCAT 
TTCCACTTTT ACGAGATTAA TGGCATCACT GCAGCACTGG CTGAGGAACT CTTCGAAAAA 
GGAGAACAGC TCCTGGGGGC TGGCGAGGTC TTCGCCATTG GCCCCCTGCA GCTCTACGCA 
GTCACCGAGC AGCTGCAGCA GGGAAAGCCC ACGTGCGCCA GCGGGGATGC CAAGACTGAC 
CTGGGGCACA TCCTGGACTT CACCTGTCGC CTTAAGTACC TTAAGGTTTC TGGCACAGAA 
GGACCTTTTG GGACCAGCAA CATTCAGGAG CAGCTTCTGC CTTTTGATCT GTCTATATTC 
AAGTCTCTTC ATCAGGTGGA GATAAGTCAC TGTGATGCCA GGCGCATCCG GGGGCTGGTT 
GCATCCAAGC CCACCTTGGC CACAATGAGC GTCCGTTTCT CAGCAACCTC CATGAAGGAA 
GTCCTCGTTC CCGAAGCCTC GGAGTTCGAT GAGTGGGAGC CAGCAGGCGC AGCCCTGGAG 
GGCCCCGTGA CTGCCGTCAT CCCCACATGG CAGGCTCTGA CCGCTCTGGA CTTGAGTCAC 
AATAGCATCT CTGAGATCGA TGATTCTGTG AAACTGATTC CAAAGATTGA GTTCCTGGAC 
CTGAGTCACA ATGGAGTGCT GGTCATGAAC AATTTGCAGC ACCTGTACAA CCTTGTGCAC 
GTGGACCTGT CGTACAACAA GCTCTCCTCC TTGGAAGGGG CTCACACCAA ACTGGGCAAC 
ATCAAGACCC TGAACCTGGC GGGCAACCTC CTGAGGCATC TGAGTGGCCT GCATAAGCTC 
TATTCCCTGG TCAACCTGGA CCTCAGCGAC AACAGAATCG AACAGATGGA AGAGGTCAGA 
AGCATCGGCA GCCTCCCATG TCTGGAGCAC GTGGCTCTGC TGAACAACCC CCTGAGTATC 
ATCCCCGACT ATCGGACCAA GGTGCTGGCT CAGTTCGGAG AGCGGGCCTC AGAGGTCTGC 
CTGGACAACA CAGTGACCAC AGAGAAAGAG TTGGACACAG TGGAAGTGCT AAAAGCAATT 
CAGAAAGCCA AAGAGGTCAA GTCCAAATTA AGCAACCCTG AGAAGAAGGT CAGTGAGGAT 
TCTCGGCTCT CTGCTGCCCC CTGTGTCAGA CCCAGCAGCT CCCCACCCAG CGCGGCTCCC 
ACCTCTGCCT CCCTGCCCCA GCCCATCCTC TCCAACCAAG GCATCCTCGG AGATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NISCH ( XM_024983242.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839010.1 Bos taurus nischarin (NISCH), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa229292
Clone ID Related Accession (Same CDS sequence)	XM_024983242.1
Accession Version	XM_024983242.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	nischarin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGCGCAG CTTCGGGCCC GAGCGGGAGG CCGAGCCGGT CAAGACGGCG 
CGCGTCGTGG GCTCTGAGCT CGTGGACACG TATACGGTTT ACATCATCCA GATCACTGAT 
GGGAGCCATG AGTGGACAGT CAAGCACCGC TACAGTGATT TCCACGACCT ACATGAAAAG 
CTTGTTGCAG AGAAGAAGAT TGATAGAAGT CTGCTTCCGC CCAAAAAGAT AATTGGGAAA 
AACTCCAGGA GCTTGGTGGA AAAGAGGGAG AAGGATCTAG AAATTTATCT CCAGACGCTC 
CTGGCCACCT TCCCTGATGT GGCCCCCAGA GTGCTGGCCC AGTTCTTGCA TTTCCACTTT 
TACGAGATTA ATGGCATCAC TGCAGCACTG GCTGAGGAAC TCTTCGAAAA AGGAGAACAG 
CTCCTGGGGG CTGGCGAGGT CTTCGCCATT GGCCCCCTGC AGCTCTACGC AGTCACCGAG 
CAGCTGCAGC AGGGAAAGCC CACGTGCGCC AGCGGGGATG CCAAGACTGA CCTGGGGCAC 
ATCCTGGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAGA AGGACCTTTT 
GGGACCAGCA ACATTCAGGA GCAGCTTCTG CCTTTTGATC TGTCTATATT CAAGTCTCTT 
CATCAGGTGG AGATAAGTCA CTGTGATGCC AGGCGCATCC GGGGGCTGGT TGCATCCAAG 
CCCACCTTGG CCACAATGAG CGTCCGTTTC TCAGCAACCT CCATGAAGGA AGTCCTCGTT 
CCCGAAGCCT CGGAGTTCGA TGAGTGGGAG CCAGCAGGCG CAGCCCTGGA GGGCCCCGTG 
ACTGCCGTCA TCCCCACATG GCAGGCTCTG ACCGCTCTGG ACTTGAGTCA CAATAGCATC 
TCTGAGATCG ATGATTCTGT GAAACTGATT CCAAAGATTG AGTTCCTGGA CCTGAGTCAC 
AATGGAGTGC TGGTCATGAA CAATTTGCAG CACCTGTACA ACCTTGTGCA CGTGGACCTG 
TCGTACAACA AGCTCTCCTC CTTGGAAGGG GCTCACACCA AACTGGGCAA CATCAAGACC 
CTGAACCTGG CGGGCAACCT CCTGAGGCAT CTGAGTGGCC TGCATAAGCT CTATTCCCTG 
GTCAACCTGG ACCTCAGCGA CAACAGAATC GAACAGATGG AAGAGGTCAG AAGCATCGGC 
AGCCTCCCAT GTCTGGAGCA CGTGGCTCTG CTGAACAACC CCCTGAGTAT CATCCCCGAC 
TATCGGACCA AGGTGCTGGC TCAGTTCGGA GAGCGGGCCT CAGAGGTCTG CCTGGACAAC 
ACAGTGACCA CAGAGAAAGA GTTGGACACA GTGGAAGTGC TAAAAGCAAT TCAGAAAGCC 
AAAGAGGTCA AGTCCAAATT AAGCAACCCT GAGAAGAAGG TCAGTGAGGA TTCTCGGCTC 
TCTGCTGCCC CCTGTGTCAG ACCCAGCAGC TCCCCACCCA GCGCGGCTCC CACCTCTGCC 
TCCCTGCCCC AGCCCATCCT CTCCAACCAA GGCATCCTCG GAGATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839010.1
CDS	77..1627
Translation	MAAAARSFGPEREAEPVKTARVVGSELVDTYTVYIIQITDGSHEWTVKHRYSDFHDLHEKLVAEKKIDRSLLPPKKIIGKNSRSLVEKREKDLEIYLQTLLATFPDVAPRVLAQFLHFHFYEINGITAALAEELFEKGEQLLGAGEVFAIGPLQLYAVTEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIQEQLLPFDLSIFKSLHQVEISHCDARRIRGLVASKPTLATMSVRFSATSMKEVLVPEASEFDEWEPAGAALEGPVTAVIPTWQALTALDLSHNSISEIDDSVKLIPKIEFLDLSHNGVLVMNNLQHLYNLVHVDLSYNKLSSLEGAHTKLGNIKTLNLAGNLLRHLSGLHKLYSLVNLDLSDNRIEQMEEVRSIGSLPCLEHVALLNNPLSIIPDYRTKVLAQFGERASEVCLDNTVTTEKELDTVEVLKAIQKAKEVKSKLSNPEKKVSEDSRLSAAPCVRPSSSPPSAAPTSASLPQPILSNQGILGDE

Target ORF information:

RefSeq Version	XM_024983242.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nischarin (NISCH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983242.1

ORF Insert Sequence:

ATGGCGGCGG CGGCGCGCAG CTTCGGGCCC GAGCGGGAGG CCGAGCCGGT CAAGACGGCG 
CGCGTCGTGG GCTCTGAGCT CGTGGACACG TATACGGTTT ACATCATCCA GATCACTGAT 
GGGAGCCATG AGTGGACAGT CAAGCACCGC TACAGTGATT TCCACGACCT ACATGAAAAG 
CTTGTTGCAG AGAAGAAGAT TGATAGAAGT CTGCTTCCGC CCAAAAAGAT AATTGGGAAA 
AACTCCAGGA GCTTGGTGGA AAAGAGGGAG AAGGATCTAG AAATTTATCT CCAGACGCTC 
CTGGCCACCT TCCCTGATGT GGCCCCCAGA GTGCTGGCCC AGTTCTTGCA TTTCCACTTT 
TACGAGATTA ATGGCATCAC TGCAGCACTG GCTGAGGAAC TCTTCGAAAA AGGAGAACAG 
CTCCTGGGGG CTGGCGAGGT CTTCGCCATT GGCCCCCTGC AGCTCTACGC AGTCACCGAG 
CAGCTGCAGC AGGGAAAGCC CACGTGCGCC AGCGGGGATG CCAAGACTGA CCTGGGGCAC 
ATCCTGGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAGA AGGACCTTTT 
GGGACCAGCA ACATTCAGGA GCAGCTTCTG CCTTTTGATC TGTCTATATT CAAGTCTCTT 
CATCAGGTGG AGATAAGTCA CTGTGATGCC AGGCGCATCC GGGGGCTGGT TGCATCCAAG 
CCCACCTTGG CCACAATGAG CGTCCGTTTC TCAGCAACCT CCATGAAGGA AGTCCTCGTT 
CCCGAAGCCT CGGAGTTCGA TGAGTGGGAG CCAGCAGGCG CAGCCCTGGA GGGCCCCGTG 
ACTGCCGTCA TCCCCACATG GCAGGCTCTG ACCGCTCTGG ACTTGAGTCA CAATAGCATC 
TCTGAGATCG ATGATTCTGT GAAACTGATT CCAAAGATTG AGTTCCTGGA CCTGAGTCAC 
AATGGAGTGC TGGTCATGAA CAATTTGCAG CACCTGTACA ACCTTGTGCA CGTGGACCTG 
TCGTACAACA AGCTCTCCTC CTTGGAAGGG GCTCACACCA AACTGGGCAA CATCAAGACC 
CTGAACCTGG CGGGCAACCT CCTGAGGCAT CTGAGTGGCC TGCATAAGCT CTATTCCCTG 
GTCAACCTGG ACCTCAGCGA CAACAGAATC GAACAGATGG AAGAGGTCAG AAGCATCGGC 
AGCCTCCCAT GTCTGGAGCA CGTGGCTCTG CTGAACAACC CCCTGAGTAT CATCCCCGAC 
TATCGGACCA AGGTGCTGGC TCAGTTCGGA GAGCGGGCCT CAGAGGTCTG CCTGGACAAC 
ACAGTGACCA CAGAGAAAGA GTTGGACACA GTGGAAGTGC TAAAAGCAAT TCAGAAAGCC 
AAAGAGGTCA AGTCCAAATT AAGCAACCCT GAGAAGAAGG TCAGTGAGGA TTCTCGGCTC 
TCTGCTGCCC CCTGTGTCAG ACCCAGCAGC TCCCCACCCA GCGCGGCTCC CACCTCTGCC 
TCCCTGCCCC AGCCCATCCT CTCCAACCAA GGCATCCTCG GAGATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NISCH ( XM_015468772.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324258.1 Bos taurus nischarin (NISCH), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa95139
Clone ID Related Accession (Same CDS sequence)	XM_015468772.1
Accession Version	XM_015468772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LOW QUALITY PROTEIN: nischarin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGGCGGCGG CGGCGCGCAG CTTCGGGCCC GAGCGGGAGG CCGAGCGCGG TCAAGACGGC 
GCGCGTCGTG GGCTCTGAGC TCGTGACACG TATACGGTTT ACATCATCCA GATCACTGAT 
GGGAGCCATG AGTGGACAGT CAAGCACCGC TACAGTGATT TCCACGACCT ACATGAAAAG 
CTTGTTGCAG AGAAGAAGAT TGATAGAAGT CTGCTTCCGC CCAAAAAGAT AATTGGGAAA 
AACTCCAGGA GCTTGGTGGA AAAGAGGGAG AAGGATCTAG AAATTTATCT CCAGACGCTC 
CTGGCCACCT TCCCTGATGT GGCCCCCAGA GTGCTGGCCC AGTTCTTGCA TTTCCACTTT 
TACGAGATTA ATGGCATCAC TGCAGCACTG GCTGAGGAAC TCTTCGAAAA AGGAGAACAG 
CTCCTGGGGG CTGGCGAGGT CTTCGCCATT GGCCCCCTGC AGCTCTACGC AGTCACCGAG 
CAGCTGCAGC AGGGAAAGCC CACGTGCGCC AGCGGGGATG CCAAGACTGA CCTGGGGCAC 
ATCCTGGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAGA AGGACCTTTT 
GGGACCAGCA ACATTCAGGA GCAGCTTCTG CCTTTTGATC TGTCTATATT CAAGTCTCTT 
CATCAGGTGG AGATAAGTCA CTGTGATGCC AGGCGCATCC GGGGGCTGGT TGCATCCAAG 
CCCACCTTGG CCACAATGAG CGTCCGTTTC TCAGCAACCT CCATGAAGGA AGTCCTCGTT 
CCCGAAGCCT CGGAGTTCGA TGAGTGGGAG CCAGCAGGCG CAGCCCTGGA GGGCCCCGTG 
ACTGCCGTCA TCCCCACATG GCAGGCTCTG ACCGCTCTGG ACTTGAGTCA CAATAGCATC 
TCTGAGATCG ATGATTCTGT GAAACTGATT CCAAAGATTG AGTTCCTGGA CCTGAGTCAC 
AATGGAGTGC TGGTCATGAA CAATTTGCAG CACCTGTACA ACCTTGTGCA CGTGGACCTG 
TCGTACAACA AGCTCTCCTC CTTGGAAGGG GCTCACACCA AACTGGGCAA CATCAAGACC 
CTGAACCTGG CGGGCAACCT CCTGAGGCAT CTGAGTGGCC TGCATAAGCT CTATTCCCTG 
GTCAACCTGG ACCTCAGCGA CAACAGAATC GAACAGATGG AAGAGGTCAG AAGCATCGGC 
AGCCTCCCAT GTCTGGAGCA CGTGGCTCTG CTGAACAACC CCCTGAGTAT CATCCCCGAC 
TATCGGACCA AGGTGCTGGC TCAGTTCGGA GAGCGGGCCT CAGAGGTCTG CCTGGACAAC 
ACAGTGACCA CAGAGAAAGA GTTGGACACA GTGGAAGTGC TAAAAGCAAT TCAGAAAGCC 
AAAGAGGTCA AGTCCAAATT AAGCAACCCT GAGAAGAAGG TCAGTGAGGA TTCTCGGCTC 
TCTGCTGCCC CCTGTGTCAG ACCCAGCAGC TCCCCACCCA GCGCGGCTCC CACCTCTGCC 
TCCCTGCCCC AGCCCATCCT CTCCAACCAA GGCATCCTCG GAGATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324258.1
CDS	61..1611
Translation	MAAAARSFGPEREAERGQDGARRGL*ARDTYTVYIIQITDGSHEWTVKHRYSDFHDLHEKLVAEKKIDRSLLPPKKIIGKNSRSLVEKREKDLEIYLQTLLATFPDVAPRVLAQFLHFHFYEINGITAALAEELFEKGEQLLGAGEVFAIGPLQLYAVTEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIQEQLLPFDLSIFKSLHQVEISHCDARRIRGLVASKPTLATMSVRFSATSMKEVLVPEASEFDEWEPAGAALEGPVTAVIPTWQALTALDLSHNSISEIDDSVKLIPKIEFLDLSHNGVLVMNNLQHLYNLVHVDLSYNKLSSLEGAHTKLGNIKTLNLAGNLLRHLSGLHKLYSLVNLDLSDNRIEQMEEVRSIGSLPCLEHVALLNNPLSIIPDYRTKVLAQFGERASEVCLDNTVTTEKELDTVEVLKAIQKAKEVKSKLSNPEKKVSEDSRLSAAPCVRPSSSPPSAAPTSASLPQPILSNQGILGDE

Target ORF information:

RefSeq Version	XM_015468772.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nischarin (NISCH), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468772.1

ORF Insert Sequence:

ATGGCGGCGG CGGCGCGCAG CTTCGGGCCC GAGCGGGAGG CCGAGCGCGG TCAAGACGGC 
GCGCGTCGTG GGCTCTGAGC TCGTGACACG TATACGGTTT ACATCATCCA GATCACTGAT 
GGGAGCCATG AGTGGACAGT CAAGCACCGC TACAGTGATT TCCACGACCT ACATGAAAAG 
CTTGTTGCAG AGAAGAAGAT TGATAGAAGT CTGCTTCCGC CCAAAAAGAT AATTGGGAAA 
AACTCCAGGA GCTTGGTGGA AAAGAGGGAG AAGGATCTAG AAATTTATCT CCAGACGCTC 
CTGGCCACCT TCCCTGATGT GGCCCCCAGA GTGCTGGCCC AGTTCTTGCA TTTCCACTTT 
TACGAGATTA ATGGCATCAC TGCAGCACTG GCTGAGGAAC TCTTCGAAAA AGGAGAACAG 
CTCCTGGGGG CTGGCGAGGT CTTCGCCATT GGCCCCCTGC AGCTCTACGC AGTCACCGAG 
CAGCTGCAGC AGGGAAAGCC CACGTGCGCC AGCGGGGATG CCAAGACTGA CCTGGGGCAC 
ATCCTGGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAGA AGGACCTTTT 
GGGACCAGCA ACATTCAGGA GCAGCTTCTG CCTTTTGATC TGTCTATATT CAAGTCTCTT 
CATCAGGTGG AGATAAGTCA CTGTGATGCC AGGCGCATCC GGGGGCTGGT TGCATCCAAG 
CCCACCTTGG CCACAATGAG CGTCCGTTTC TCAGCAACCT CCATGAAGGA AGTCCTCGTT 
CCCGAAGCCT CGGAGTTCGA TGAGTGGGAG CCAGCAGGCG CAGCCCTGGA GGGCCCCGTG 
ACTGCCGTCA TCCCCACATG GCAGGCTCTG ACCGCTCTGG ACTTGAGTCA CAATAGCATC 
TCTGAGATCG ATGATTCTGT GAAACTGATT CCAAAGATTG AGTTCCTGGA CCTGAGTCAC 
AATGGAGTGC TGGTCATGAA CAATTTGCAG CACCTGTACA ACCTTGTGCA CGTGGACCTG 
TCGTACAACA AGCTCTCCTC CTTGGAAGGG GCTCACACCA AACTGGGCAA CATCAAGACC 
CTGAACCTGG CGGGCAACCT CCTGAGGCAT CTGAGTGGCC TGCATAAGCT CTATTCCCTG 
GTCAACCTGG ACCTCAGCGA CAACAGAATC GAACAGATGG AAGAGGTCAG AAGCATCGGC 
AGCCTCCCAT GTCTGGAGCA CGTGGCTCTG CTGAACAACC CCCTGAGTAT CATCCCCGAC 
TATCGGACCA AGGTGCTGGC TCAGTTCGGA GAGCGGGCCT CAGAGGTCTG CCTGGACAAC 
ACAGTGACCA CAGAGAAAGA GTTGGACACA GTGGAAGTGC TAAAAGCAAT TCAGAAAGCC 
AAAGAGGTCA AGTCCAAATT AAGCAACCCT GAGAAGAAGG TCAGTGAGGA TTCTCGGCTC 
TCTGCTGCCC CCTGTGTCAG ACCCAGCAGC TCCCCACCCA GCGCGGCTCC CACCTCTGCC 
TCCCTGCCCC AGCCCATCCT CTCCAACCAA GGCATCCTCG GAGATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NISCH ( XM_024983241.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839009.1 Bos taurus nischarin (NISCH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa229291
Clone ID Related Accession (Same CDS sequence)	XM_024983241.1
Accession Version	XM_024983241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	nischarin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGCGCAG CTTCGGGCCC GAGCGGGAGG CCGAGCCGGT CAAGACGGCG 
CGCGTCGTGG GCTCTGAGCT CGTGGACACG TATACGGTTT ACATCATCCA GATCACTGAT 
GGGAGCCATG AGTGGACAGT CAAGCACCGC TACAGTGATT TCCACGACCT ACATGAAAAG 
CTTGTTGCAG AGAAGAAGAT TGATAGAAGT CTGCTTCCGC CCAAAAAGAT AATTGGGAAA 
AACTCCAGGA GCTTGGTGGA AAAGAGGGAG AAGGATCTAG AAATTTATCT CCAGACGCTC 
CTGGCCACCT TCCCTGATGT GGCCCCCAGA GTGCTGGCCC AGTTCTTGCA TTTCCACTTT 
TACGAGATTA ATGGCATCAC TGCAGCACTG GCTGAGGAAC TCTTCGAAAA AGGAGAACAG 
CTCCTGGGGG CTGGCGAGGT CTTCGCCATT GGCCCCCTGC AGCTCTACGC AGTCACCGAG 
CAGCTGCAGC AGGGAAAGCC CACGTGCGCC AGCGGGGATG CCAAGACTGA CCTGGGGCAC 
ATCCTGGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAGA AGGACCTTTT 
GGGACCAGCA ACATTCAGGA GCAGCTTCTG CCTTTTGATC TGTCTATATT CAAGTCTCTT 
CATCAGGTGG AGATAAGTCA CTGTGATGCC AGGCGCATCC GGGGGCTGGT TGCATCCAAG 
CCCACCTTGG CCACAATGAG CGTCCGTTTC TCAGCAACCT CCATGAAGGA AGTCCTCGTT 
CCCGAAGCCT CGGAGTTCGA TGAGTGGGAG CCAGCAGGCG CAGCCCTGGA GGGCCCCGTG 
ACTGCCGTCA TCCCCACATG GCAGGCTCTG ACCGCTCTGG ACTTGAGTCA CAATAGCATC 
TCTGAGATCG ATGATTCTGT GAAACTGATT CCAAAGATTG AGTTCCTGGA CCTGAGTCAC 
AATGGAGTGC TGGTCATGAA CAATTTGCAG CACCTGTACA ACCTTGTGCA CGTGGACCTG 
TCGTACAACA AGCTCTCCTC CTTGGAAGGG GCTCACACCA AACTGGGCAA CATCAAGACC 
CTGAACCTGG CGGGCAACCT CCTGAGGCAT CTGAGTGGCC TGCATAAGCT CTATTCCCTG 
GTCAACCTGG ACCTCAGCGA CAACAGAATC GAACAGATGG AAGAGGTCAG AAGCATCGGC 
AGCCTCCCAT GTCTGGAGCA CGTGGCTCTG CTGAACAACC CCCTGAGTAT CATCCCCGAC 
TATCGGACCA AGGTGCTGGC TCAGTTCGGA GAGCGGGCCT CAGAGGTCTG CCTGGACAAC 
ACAGTGACCA CAGAGAAAGA GTTGGACACA GTGGAAGTGC TAAAAGCAAT TCAGAAAGCC 
AAAGAGGTCA AGTCCAAATT AAGCAACCCT GAGAAGAAGG TCAGTGAGGA TTCTCGGCTC 
TCTGCTGCCC CCTGTGTCAG ACCCAGCAGC TCCCCACCCA GCGCGGCTCC CACCTCTGCC 
TCCCTGCCCC AGCCCATCCT CTCCAACCAA GGAATCATGT TCGTGCAAGA GGATGCCCTG 
GCCAGCAGCC TCTCATCCAC CGACAGTCTG ACTCCTGAGG ACCGGTCCAT TGTGGGAGGA 
TGCTCTGATT CCTTGGAGTC CATCCCTGCG GGACAGGCAC CTTCTGATGA TTTAAGGGAC 
GTGCCGGGAG CTGTTGGCGG TGTGAGCCCC GAGCACGCGG AGCCAGAGGT CCAGGTGGTG 
CCTGGGTCCG GCCAGATCAT CTTCCTGCCC TTTACCTGCA TTGGCTACAC TGCCACCAAC 
CAGGACTTCA TCCAGCGCCT GAGCACGCTC ATCCGACAGG CTATCGAGCG GCAGCTCCCT 
GCTTGGATTG AGGCCGCCAA CCAGCGAGAG GAGGGCCAGG GGGAGCAGGG CGAGGATGAT 
GAGGAGGAGG AGGACGTTGC TGAGAATCGC TACTTCGAAA TGGGGCCCCC AGATACAGAG 
GAAGAGGAGG AGGGTGGTCC AGGAGAGGAA GAGGAGGAAG AGGAGGAGGA GGAGGCTGAG 
GAGGAGCGCC TGGCTCTGGA GTGGGCGCTG GGCGCAGACG AGGACTTCCT GCTGGAGCAC 
ATCCGCATCC TCAAGGTGCT CTGGTGCTTC CTGATCCACG TGCAGGGCAG CATCCGCCAG 
TTCGCCGCCT GCCTCGTACT CACCGACTTC GGCATCGCTG TCTTCGAGAT CCCGCACCAG 
GAGTCGCGGG GCAGCAGCCA GCACATCCTC TCCTCGCTGC GCTTCGTGTT CTGCTTCCCG 
CACGGCGACC TCACCGAGTT CGGCTTCCTC ATGCCGGAGC TGTGCCTGGT GCTGAAGGTG 
CGGCACAGTG AGAACACGCT CTTCATCATC TCCGACGCTG CCCACCTGCA CGAGTTCCAT 
GCCGACCTGC GCGCCTGCTT CGCCCCGCAG CACATGGCCA TGCTGTGCAG CCCCGTGCTT 
TACGGCAGCC ACACCAGCCT GCAGGAGTTC CTGCGCCAGC TGCTCACCTT CTACAAAGTG 
GCAGGCGGCT GCCAGGAGCG CAGCCAGGGC TGCTTTCCCG TCTACCTGGT CTACAGTGAC 
AAGCGCATGG TGCAGACGGC CCCTGGGGAC TACTCGGGCA ACATCGAGTG GGCCAGCTGC 
ACCCTCTGCT CGGCCGTGCG GCGCTCCTGC TGCGCGCCCT CCGAGGCCGT TAAGTCCGCC 
GCCATCCCCT ACTGGCTGCT GCTCACGCCC CAGCACCTCA ACGTCATCAA GGCCGACTTC 
AACCCCATGC CCAACCGGGG CACCCACAAC TGCCGCAACC GCAACAGCTT CAAGCTCAGC 
CGTGTGCCGC TGTCCACTGT GCTGCTGGAC CCCACGCGCA GCTGCACCCA GCCACGGGGT 
GCCTTCGCGG ACGGCCACGT GCTCGAGCTG CTCGTGGGCT ACCGCTTCGT CACCGCCATC 
TTTGTGCTGC CCCATGAGAA GTTCCACTTC CTGCGCATCT ACAACCAGCT GCGGGCCTCG 
CTGCAGGACC TGAAGACCGT GGTCATCGCC AAGACCCCCG CAACGGGGGG CGGGCCTCAG 
AGCCCCCTCG TGGACGGCCA GCCTGCAGAG AGCAGGGCCA GCAGTGACCA GAATCTCCAA 
GAAGCTCCAG CAGAGACTAC AGCCCCGGCC CTGGTGGAGG CCCCTGCTCT TACTCCAGGA 
GAGGCCTCAG CCCCAAGCCC AGCAAAGACC CCAGCTCCAG TGGAGGCCTC AGTGCCAGCT 
CTTGTTCCAG TGGAGGTCCC CGAGGAGGCC GAGGCCTCAG CAGAGATCCC AGTGGAGGCT 
CTGGCCCAAG CAGAGGCCCC TGCACAGTAC CCAAGTGAGC CCCTGATCCG GTCCATGTCG 
GAGGACAATC AGATCCCGTC ACACCTGCCT GCCTGCCCGT CTCTCCGGCA CATCGCCAGC 
CTGCGTGGAC GGGCCGTGGT CGAGTTCTTC CACAGCAGCA TTGCTGAGGT GGAAAATGAG 
GAGCTGCGGC ACCTCCTGTG GTCATCGGTG GTGTTCTACC AGATGCCGGG GCTGGAGGTG 
ACAGCCTGTG TGCTGCTCTC TACCAAGGCC GTGTACTTCG TGCTGCACGA CGGCCTCCGC 
CGCTACTTCT CGGAGCCGCT GCAGGATTTC TGGCATCAGA AAAACACCGA CTACAACAAC 
AGCCCCTTCC ACATCTCTCA GTGCTTTGTT TTGAAACTCA GCGACCTGCA GGTAGTCAAC 
GTTGGGCTGT TTGACCAGTA TTTCCGGCTA ACAGGCTCCT GCCCGGCGCA GGTGGTTACC 
TGCTTGACGC GGGACAGCTA CCTGACCCAC TGCTTCCTCC AGCACCTCAT GGCTGTGCTC 
TCCTTGCTGG AACGCACACC CTCACCTGAG CCTGTGGACA AAGACTTCTA TTCTGAGTTT 
GGAAACAAGA CCACAGGGAA GATGGAAAAC TATGAGCTGA TCCACTCGAG CCGCGTCAAG 
TTCACCTACC CCAGCGAGGA GGAGGTCGGA GACCTGACGT TCACCGTGGC CCAGAAGATG 
GCTGCGCCGG AGAAGGCGCC AGCCCTCAGC ATCCTGCTGT GTGTGCAGGC CTTCCAGGTG 
GGCTCGCCGC AGCCGGGGCG CTGCAGGGGC TTGCTGCGCC CCAAGACGCT CCTGCTCACC 
AGCGCCGAGA TCTTCCTCCT GGACGAGGAC TTCGTCCACT ACCCGCTGCC CGAGTTCGCC 
AAAGAGCCGC CACAGAGGGA CCGGTACCGG CTGGATGACG GGCGCCGCGT CCGGGACCTG 
GACCGCGTGC TCATGGGCTA CCAGACATAC CCACAGGCCC TCACCCTGGT CTTCGATGAT 
GTGCAGGGCC ACGACCTCAT GGGCAGCGTC ACCCTGGACC ACTTCGGGGA GGTGCCAGGT 
GGCCCGGCTA GGGGTGGCCA GGGCCGTGAG GTCCAGTGGC AGGTGTTTGT CCCCAGCGCG 
GAGAGCCGAG AGAAGCTCAT CTCGCTGCTG GCCCGTCAGT GGGAGGCCCT GTGTGGCCGG 
GAGCTGCCCG TGGAGCTCAC CGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839009.1
CDS	148..4674
Translation	MAAAARSFGPEREAEPVKTARVVGSELVDTYTVYIIQITDGSHEWTVKHRYSDFHDLHEKLVAEKKIDRSLLPPKKIIGKNSRSLVEKREKDLEIYLQTLLATFPDVAPRVLAQFLHFHFYEINGITAALAEELFEKGEQLLGAGEVFAIGPLQLYAVTEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIQEQLLPFDLSIFKSLHQVEISHCDARRIRGLVASKPTLATMSVRFSATSMKEVLVPEASEFDEWEPAGAALEGPVTAVIPTWQALTALDLSHNSISEIDDSVKLIPKIEFLDLSHNGVLVMNNLQHLYNLVHVDLSYNKLSSLEGAHTKLGNIKTLNLAGNLLRHLSGLHKLYSLVNLDLSDNRIEQMEEVRSIGSLPCLEHVALLNNPLSIIPDYRTKVLAQFGERASEVCLDNTVTTEKELDTVEVLKAIQKAKEVKSKLSNPEKKVSEDSRLSAAPCVRPSSSPPSAAPTSASLPQPILSNQGIMFVQEDALASSLSSTDSLTPEDRSIVGGCSDSLESIPAGQAPSDDLRDVPGAVGGVSPEHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREEGQGEQGEDDEEEEDVAENRYFEMGPPDTEEEEEGGPGEEEEEEEEEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAAHLHEFHADLRACFAPQHMAMLCSPVLYGSHTSLQEFLRQLLTFYKVAGGCQERSQGCFPVYLVYSDKRMVQTAPGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTPQHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRIYNQLRASLQDLKTVVIAKTPATGGGPQSPLVDGQPAESRASSDQNLQEAPAETTAPALVEAPALTPGEASAPSPAKTPAPVEASVPALVPVEVPEEAEASAEIPVEALAQAEAPAQYPSEPLIRSMSEDNQIPSHLPACPSLRHIASLRGRAVVEFFHSSIAEVENEELRHLLWSSVVFYQMPGLEVTACVLLSTKAVYFVLHDGLRRYFSEPLQDFWHQKNTDYNNSPFHISQCFVLKLSDLQVVNVGLFDQYFRLTGSCPAQVVTCLTRDSYLTHCFLQHLMAVLSLLERTPSPEPVDKDFYSEFGNKTTGKMENYELIHSSRVKFTYPSEEEVGDLTFTVAQKMAAPEKAPALSILLCVQAFQVGSPQPGRCRGLLRPKTLLLTSAEIFLLDEDFVHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYQTYPQALTLVFDDVQGHDLMGSVTLDHFGEVPGGPARGGQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

Target ORF information:

RefSeq Version	XM_024983241.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nischarin (NISCH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983241.1

ORF Insert Sequence:

ATGGCGGCGG CGGCGCGCAG CTTCGGGCCC GAGCGGGAGG CCGAGCCGGT CAAGACGGCG 
CGCGTCGTGG GCTCTGAGCT CGTGGACACG TATACGGTTT ACATCATCCA GATCACTGAT 
GGGAGCCATG AGTGGACAGT CAAGCACCGC TACAGTGATT TCCACGACCT ACATGAAAAG 
CTTGTTGCAG AGAAGAAGAT TGATAGAAGT CTGCTTCCGC CCAAAAAGAT AATTGGGAAA 
AACTCCAGGA GCTTGGTGGA AAAGAGGGAG AAGGATCTAG AAATTTATCT CCAGACGCTC 
CTGGCCACCT TCCCTGATGT GGCCCCCAGA GTGCTGGCCC AGTTCTTGCA TTTCCACTTT 
TACGAGATTA ATGGCATCAC TGCAGCACTG GCTGAGGAAC TCTTCGAAAA AGGAGAACAG 
CTCCTGGGGG CTGGCGAGGT CTTCGCCATT GGCCCCCTGC AGCTCTACGC AGTCACCGAG 
CAGCTGCAGC AGGGAAAGCC CACGTGCGCC AGCGGGGATG CCAAGACTGA CCTGGGGCAC 
ATCCTGGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAGA AGGACCTTTT 
GGGACCAGCA ACATTCAGGA GCAGCTTCTG CCTTTTGATC TGTCTATATT CAAGTCTCTT 
CATCAGGTGG AGATAAGTCA CTGTGATGCC AGGCGCATCC GGGGGCTGGT TGCATCCAAG 
CCCACCTTGG CCACAATGAG CGTCCGTTTC TCAGCAACCT CCATGAAGGA AGTCCTCGTT 
CCCGAAGCCT CGGAGTTCGA TGAGTGGGAG CCAGCAGGCG CAGCCCTGGA GGGCCCCGTG 
ACTGCCGTCA TCCCCACATG GCAGGCTCTG ACCGCTCTGG ACTTGAGTCA CAATAGCATC 
TCTGAGATCG ATGATTCTGT GAAACTGATT CCAAAGATTG AGTTCCTGGA CCTGAGTCAC 
AATGGAGTGC TGGTCATGAA CAATTTGCAG CACCTGTACA ACCTTGTGCA CGTGGACCTG 
TCGTACAACA AGCTCTCCTC CTTGGAAGGG GCTCACACCA AACTGGGCAA CATCAAGACC 
CTGAACCTGG CGGGCAACCT CCTGAGGCAT CTGAGTGGCC TGCATAAGCT CTATTCCCTG 
GTCAACCTGG ACCTCAGCGA CAACAGAATC GAACAGATGG AAGAGGTCAG AAGCATCGGC 
AGCCTCCCAT GTCTGGAGCA CGTGGCTCTG CTGAACAACC CCCTGAGTAT CATCCCCGAC 
TATCGGACCA AGGTGCTGGC TCAGTTCGGA GAGCGGGCCT CAGAGGTCTG CCTGGACAAC 
ACAGTGACCA CAGAGAAAGA GTTGGACACA GTGGAAGTGC TAAAAGCAAT TCAGAAAGCC 
AAAGAGGTCA AGTCCAAATT AAGCAACCCT GAGAAGAAGG TCAGTGAGGA TTCTCGGCTC 
TCTGCTGCCC CCTGTGTCAG ACCCAGCAGC TCCCCACCCA GCGCGGCTCC CACCTCTGCC 
TCCCTGCCCC AGCCCATCCT CTCCAACCAA GGAATCATGT TCGTGCAAGA GGATGCCCTG 
GCCAGCAGCC TCTCATCCAC CGACAGTCTG ACTCCTGAGG ACCGGTCCAT TGTGGGAGGA 
TGCTCTGATT CCTTGGAGTC CATCCCTGCG GGACAGGCAC CTTCTGATGA TTTAAGGGAC 
GTGCCGGGAG CTGTTGGCGG TGTGAGCCCC GAGCACGCGG AGCCAGAGGT CCAGGTGGTG 
CCTGGGTCCG GCCAGATCAT CTTCCTGCCC TTTACCTGCA TTGGCTACAC TGCCACCAAC 
CAGGACTTCA TCCAGCGCCT GAGCACGCTC ATCCGACAGG CTATCGAGCG GCAGCTCCCT 
GCTTGGATTG AGGCCGCCAA CCAGCGAGAG GAGGGCCAGG GGGAGCAGGG CGAGGATGAT 
GAGGAGGAGG AGGACGTTGC TGAGAATCGC TACTTCGAAA TGGGGCCCCC AGATACAGAG 
GAAGAGGAGG AGGGTGGTCC AGGAGAGGAA GAGGAGGAAG AGGAGGAGGA GGAGGCTGAG 
GAGGAGCGCC TGGCTCTGGA GTGGGCGCTG GGCGCAGACG AGGACTTCCT GCTGGAGCAC 
ATCCGCATCC TCAAGGTGCT CTGGTGCTTC CTGATCCACG TGCAGGGCAG CATCCGCCAG 
TTCGCCGCCT GCCTCGTACT CACCGACTTC GGCATCGCTG TCTTCGAGAT CCCGCACCAG 
GAGTCGCGGG GCAGCAGCCA GCACATCCTC TCCTCGCTGC GCTTCGTGTT CTGCTTCCCG 
CACGGCGACC TCACCGAGTT CGGCTTCCTC ATGCCGGAGC TGTGCCTGGT GCTGAAGGTG 
CGGCACAGTG AGAACACGCT CTTCATCATC TCCGACGCTG CCCACCTGCA CGAGTTCCAT 
GCCGACCTGC GCGCCTGCTT CGCCCCGCAG CACATGGCCA TGCTGTGCAG CCCCGTGCTT 
TACGGCAGCC ACACCAGCCT GCAGGAGTTC CTGCGCCAGC TGCTCACCTT CTACAAAGTG 
GCAGGCGGCT GCCAGGAGCG CAGCCAGGGC TGCTTTCCCG TCTACCTGGT CTACAGTGAC 
AAGCGCATGG TGCAGACGGC CCCTGGGGAC TACTCGGGCA ACATCGAGTG GGCCAGCTGC 
ACCCTCTGCT CGGCCGTGCG GCGCTCCTGC TGCGCGCCCT CCGAGGCCGT TAAGTCCGCC 
GCCATCCCCT ACTGGCTGCT GCTCACGCCC CAGCACCTCA ACGTCATCAA GGCCGACTTC 
AACCCCATGC CCAACCGGGG CACCCACAAC TGCCGCAACC GCAACAGCTT CAAGCTCAGC 
CGTGTGCCGC TGTCCACTGT GCTGCTGGAC CCCACGCGCA GCTGCACCCA GCCACGGGGT 
GCCTTCGCGG ACGGCCACGT GCTCGAGCTG CTCGTGGGCT ACCGCTTCGT CACCGCCATC 
TTTGTGCTGC CCCATGAGAA GTTCCACTTC CTGCGCATCT ACAACCAGCT GCGGGCCTCG 
CTGCAGGACC TGAAGACCGT GGTCATCGCC AAGACCCCCG CAACGGGGGG CGGGCCTCAG 
AGCCCCCTCG TGGACGGCCA GCCTGCAGAG AGCAGGGCCA GCAGTGACCA GAATCTCCAA 
GAAGCTCCAG CAGAGACTAC AGCCCCGGCC CTGGTGGAGG CCCCTGCTCT TACTCCAGGA 
GAGGCCTCAG CCCCAAGCCC AGCAAAGACC CCAGCTCCAG TGGAGGCCTC AGTGCCAGCT 
CTTGTTCCAG TGGAGGTCCC CGAGGAGGCC GAGGCCTCAG CAGAGATCCC AGTGGAGGCT 
CTGGCCCAAG CAGAGGCCCC TGCACAGTAC CCAAGTGAGC CCCTGATCCG GTCCATGTCG 
GAGGACAATC AGATCCCGTC ACACCTGCCT GCCTGCCCGT CTCTCCGGCA CATCGCCAGC 
CTGCGTGGAC GGGCCGTGGT CGAGTTCTTC CACAGCAGCA TTGCTGAGGT GGAAAATGAG 
GAGCTGCGGC ACCTCCTGTG GTCATCGGTG GTGTTCTACC AGATGCCGGG GCTGGAGGTG 
ACAGCCTGTG TGCTGCTCTC TACCAAGGCC GTGTACTTCG TGCTGCACGA CGGCCTCCGC 
CGCTACTTCT CGGAGCCGCT GCAGGATTTC TGGCATCAGA AAAACACCGA CTACAACAAC 
AGCCCCTTCC ACATCTCTCA GTGCTTTGTT TTGAAACTCA GCGACCTGCA GGTAGTCAAC 
GTTGGGCTGT TTGACCAGTA TTTCCGGCTA ACAGGCTCCT GCCCGGCGCA GGTGGTTACC 
TGCTTGACGC GGGACAGCTA CCTGACCCAC TGCTTCCTCC AGCACCTCAT GGCTGTGCTC 
TCCTTGCTGG AACGCACACC CTCACCTGAG CCTGTGGACA AAGACTTCTA TTCTGAGTTT 
GGAAACAAGA CCACAGGGAA GATGGAAAAC TATGAGCTGA TCCACTCGAG CCGCGTCAAG 
TTCACCTACC CCAGCGAGGA GGAGGTCGGA GACCTGACGT TCACCGTGGC CCAGAAGATG 
GCTGCGCCGG AGAAGGCGCC AGCCCTCAGC ATCCTGCTGT GTGTGCAGGC CTTCCAGGTG 
GGCTCGCCGC AGCCGGGGCG CTGCAGGGGC TTGCTGCGCC CCAAGACGCT CCTGCTCACC 
AGCGCCGAGA TCTTCCTCCT GGACGAGGAC TTCGTCCACT ACCCGCTGCC CGAGTTCGCC 
AAAGAGCCGC CACAGAGGGA CCGGTACCGG CTGGATGACG GGCGCCGCGT CCGGGACCTG 
GACCGCGTGC TCATGGGCTA CCAGACATAC CCACAGGCCC TCACCCTGGT CTTCGATGAT 
GTGCAGGGCC ACGACCTCAT GGGCAGCGTC ACCCTGGACC ACTTCGGGGA GGTGCCAGGT 
GGCCCGGCTA GGGGTGGCCA GGGCCGTGAG GTCCAGTGGC AGGTGTTTGT CCCCAGCGCG 
GAGAGCCGAG AGAAGCTCAT CTCGCTGCTG GCCCGTCAGT GGGAGGCCCT GTGTGGCCGG 
GAGCTGCCCG TGGAGCTCAC CGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NISCH ( XM_002697064.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697110.4 Bos taurus nischarin (NISCH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa94455
Clone ID Related Accession (Same CDS sequence)	XM_002697064.4
Accession Version	XM_002697064.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nischarin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002697064.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGCCA GATCCAGTGC TAGGGCCATG GAGGAGGCTA AAAGGGGCCC CCTGCTTCTC 
AGCAAGGGTC AGGTTTACAT CATCCAGATC ACTGATGGGA GCCATGAGTG GACAGTCAAG 
CACCGCTACA GTGATTTCCA CGACCTACAT GAAAAGCTTG TTGCAGAGAA GAAGATTGAT 
AGAAGTCTGC TTCCGCCCAA AAAGATAATT GGGAAAAACT CCAGGAGCTT GGTGGAAAAG 
AGGGAGAAGG ATCTAGAAAT TTATCTCCAG ACGCTCCTGG CCACCTTCCC TGATGTGGCC 
CCCAGAGTGC TGGCCCAGTT CTTGCATTTC CACTTTTACG AGATTAATGG CATCACTGCA 
GCACTGGCTG AGGAACTCTT CGAAAAAGGA GAACAGCTCC TGGGGGCTGG CGAGGTCTTC 
GCCATTGGCC CCCTGCAGCT CTACGCAGTC ACCGAGCAGC TGCAGCAGGG AAAGCCCACG 
TGCGCCAGCG GGGATGCCAA GACTGACCTG GGGCACATCC TGGACTTCAC CTGTCGCCTT 
AAGTACCTTA AGGTTTCTGG CACAGAAGGA CCTTTTGGGA CCAGCAACAT TCAGGAGCAG 
CTTCTGCCTT TTGATCTGTC TATATTCAAG TCTCTTCATC AGGTGGAGAT AAGTCACTGT 
GATGCCAGGC GCATCCGGGG GCTGGTTGCA TCCAAGCCCA CCTTGGCCAC AATGAGCGTC 
CGTTTCTCAG CAACCTCCAT GAAGGAAGTC CTCGTTCCCG AAGCCTCGGA GTTCGATGAG 
TGGGAGCCAG CAGGCGCAGC CCTGGAGGGC CCCGTGACTG CCGTCATCCC CACATGGCAG 
GCTCTGACCG CTCTGGACTT GAGTCACAAT AGCATCTCTG AGATCGATGA TTCTGTGAAA 
CTGATTCCAA AGATTGAGTT CCTGGACCTG AGTCACAATG GAGTGCTGGT CATGAACAAT 
TTGCAGCACC TGTACAACCT TGTGCACGTG GACCTGTCGT ACAACAAGCT CTCCTCCTTG 
GAAGGGGCTC ACACCAAACT GGGCAACATC AAGACCCTGA ACCTGGCGGG CAACCTCCTG 
AGGCATCTGA GTGGCCTGCA TAAGCTCTAT TCCCTGGTCA ACCTGGACCT CAGCGACAAC 
AGAATCGAAC AGATGGAAGA GGTCAGAAGC ATCGGCAGCC TCCCATGTCT GGAGCACGTG 
GCTCTGCTGA ACAACCCCCT GAGTATCATC CCCGACTATC GGACCAAGGT GCTGGCTCAG 
TTCGGAGAGC GGGCCTCAGA GGTCTGCCTG GACAACACAG TGACCACAGA GAAAGAGTTG 
GACACAGTGG AAGTGCTAAA AGCAATTCAG AAAGCCAAAG AGGTCAAGTC CAAATTAAGC 
AACCCTGAGA AGAAGGTCAG TGAGGATTCT CGGCTCTCTG CTGCCCCCTG TGTCAGACCC 
AGCAGCTCCC CACCCAGCGC GGCTCCCACC TCTGCCTCCC TGCCCCAGCC CATCCTCTCC 
AACCAAGGAA TCATGTTCGT GCAAGAGGAT GCCCTGGCCA GCAGCCTCTC ATCCACCGAC 
AGTCTGACTC CTGAGGACCG GTCCATTGTG GGAGGATGCT CTGATTCCTT GGAGTCCATC 
CCTGCGGGAC AGGCACCTTC TGATGATTTA AGGGACGTGC CGGGAGCTGT TGGCGGTGTG 
AGCCCCGAGC ACGCGGAGCC AGAGGTCCAG GTGGTGCCTG GGTCCGGCCA GATCATCTTC 
CTGCCCTTTA CCTGCATTGG CTACACTGCC ACCAACCAGG ACTTCATCCA GCGCCTGAGC 
ACGCTCATCC GACAGGCTAT CGAGCGGCAG CTCCCTGCTT GGATTGAGGC CGCCAACCAG 
CGAGAGGAGG GCCAGGGGGA GCAGGGCGAG GATGATGAGG AGGAGGAGGA CGTTGCTGAG 
AATCGCTACT TCGAAATGGG GCCCCCAGAT ACAGAGGAAG AGGAGGAGGG TGGTCCAGGA 
GAGGAAGAGG AGGAAGAGGA GGAGGAGGAG GCTGAGGAGG AGCGCCTGGC TCTGGAGTGG 
GCGCTGGGCG CAGACGAGGA CTTCCTGCTG GAGCACATCC GCATCCTCAA GGTGCTCTGG 
TGCTTCCTGA TCCACGTGCA GGGCAGCATC CGCCAGTTCG CCGCCTGCCT CGTACTCACC 
GACTTCGGCA TCGCTGTCTT CGAGATCCCG CACCAGGAGT CGCGGGGCAG CAGCCAGCAC 
ATCCTCTCCT CGCTGCGCTT CGTGTTCTGC TTCCCGCACG GCGACCTCAC CGAGTTCGGC 
TTCCTCATGC CGGAGCTGTG CCTGGTGCTG AAGGTGCGGC ACAGTGAGAA CACGCTCTTC 
ATCATCTCCG ACGCTGCCCA CCTGCACGAG TTCCATGCCG ACCTGCGCGC CTGCTTCGCC 
CCGCAGCACA TGGCCATGCT GTGCAGCCCC GTGCTTTACG GCAGCCACAC CAGCCTGCAG 
GAGTTCCTGC GCCAGCTGCT CACCTTCTAC AAAGTGGCAG GCGGCTGCCA GGAGCGCAGC 
CAGGGCTGCT TTCCCGTCTA CCTGGTCTAC AGTGACAAGC GCATGGTGCA GACGGCCCCT 
GGGGACTACT CGGGCAACAT CGAGTGGGCC AGCTGCACCC TCTGCTCGGC CGTGCGGCGC 
TCCTGCTGCG CGCCCTCCGA GGCCGTTAAG TCCGCCGCCA TCCCCTACTG GCTGCTGCTC 
ACGCCCCAGC ACCTCAACGT CATCAAGGCC GACTTCAACC CCATGCCCAA CCGGGGCACC 
CACAACTGCC GCAACCGCAA CAGCTTCAAG CTCAGCCGTG TGCCGCTGTC CACTGTGCTG 
CTGGACCCCA CGCGCAGCTG CACCCAGCCA CGGGGTGCCT TCGCGGACGG CCACGTGCTC 
GAGCTGCTCG TGGGCTACCG CTTCGTCACC GCCATCTTTG TGCTGCCCCA TGAGAAGTTC 
CACTTCCTGC GCATCTACAA CCAGCTGCGG GCCTCGCTGC AGGACCTGAA GACCGTGGTC 
ATCGCCAAGA CCCCCGCAAC GGGGGGCGGG CCTCAGAGCC CCCTCGTGGA CGGCCAGCCT 
GCAGAGAGCA GGGCCAGCAG TGACCAGAAT CTCCAAGAAG CTCCAGCAGA GACTACAGCC 
CCGGCCCTGG TGGAGGCCCC TGCTCTTACT CCAGGAGAGG CCTCAGCCCC AAGCCCAGCA 
AAGACCCCAG CTCCAGTGGA GGCCTCAGTG CCAGCTCTTG TTCCAGTGGA GGTCCCCGAG 
GAGGCCGAGG CCTCAGCAGA GATCCCAGTG GAGGCTCTGG CCCAAGCAGA GGCCCCTGCA 
CAGTACCCAA GTGAGCCCCT GATCCGGTCC ATGTCGGAGG ACAATCAGAT CCCGTCACAC 
CTGCCTGCCT GCCCGTCTCT CCGGCACATC GCCAGCCTGC GTGGACGGGC CGTGGTCGAG 
TTCTTCCACA GCAGCATTGC TGAGGTGGAA AATGAGGAGC TGCGGCACCT CCTGTGGTCA 
TCGGTGGTGT TCTACCAGAT GCCGGGGCTG GAGGTGACAG CCTGTGTGCT GCTCTCTACC 
AAGGCCGTGT ACTTCGTGCT GCACGACGGC CTCCGCCGCT ACTTCTCGGA GCCGCTGCAG 
GATTTCTGGC ATCAGAAAAA CACCGACTAC AACAACAGCC CCTTCCACAT CTCTCAGTGC 
TTTGTTTTGA AACTCAGCGA CCTGCAGGTA GTCAACGTTG GGCTGTTTGA CCAGTATTTC 
CGGCTAACAG GCTCCTGCCC GGCGCAGGTG GTTACCTGCT TGACGCGGGA CAGCTACCTG 
ACCCACTGCT TCCTCCAGCA CCTCATGGCT GTGCTCTCCT TGCTGGAACG CACACCCTCA 
CCTGAGCCTG TGGACAAAGA CTTCTATTCT GAGTTTGGAA ACAAGACCAC AGGGAAGATG 
GAAAACTATG AGCTGATCCA CTCGAGCCGC GTCAAGTTCA CCTACCCCAG CGAGGAGGAG 
GTCGGAGACC TGACGTTCAC CGTGGCCCAG AAGATGGCTG CGCCGGAGAA GGCGCCAGCC 
CTCAGCATCC TGCTGTGTGT GCAGGCCTTC CAGGTGGGCT CGCCGCAGCC GGGGCGCTGC 
AGGGGCTTGC TGCGCCCCAA GACGCTCCTG CTCACCAGCG CCGAGATCTT CCTCCTGGAC 
GAGGACTTCG TCCACTACCC GCTGCCCGAG TTCGCCAAAG AGCCGCCACA GAGGGACCGG 
TACCGGCTGG ATGACGGGCG CCGCGTCCGG GACCTGGACC GCGTGCTCAT GGGCTACCAG 
ACATACCCAC AGGCCCTCAC CCTGGTCTTC GATGATGTGC AGGGCCACGA CCTCATGGGC 
AGCGTCACCC TGGACCACTT CGGGGAGGTG CCAGGTGGCC CGGCTAGGGG TGGCCAGGGC 
CGTGAGGTCC AGTGGCAGGT GTTTGTCCCC AGCGCGGAGA GCCGAGAGAA GCTCATCTCG 
CTGCTGGCCC GTCAGTGGGA GGCCCTGTGT GGCCGGGAGC TGCCCGTGGA GCTCACCGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697110.4
CDS	293..4795
Translation	MPARSSARAMEEAKRGPLLLSKGQVYIIQITDGSHEWTVKHRYSDFHDLHEKLVAEKKIDRSLLPPKKIIGKNSRSLVEKREKDLEIYLQTLLATFPDVAPRVLAQFLHFHFYEINGITAALAEELFEKGEQLLGAGEVFAIGPLQLYAVTEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIQEQLLPFDLSIFKSLHQVEISHCDARRIRGLVASKPTLATMSVRFSATSMKEVLVPEASEFDEWEPAGAALEGPVTAVIPTWQALTALDLSHNSISEIDDSVKLIPKIEFLDLSHNGVLVMNNLQHLYNLVHVDLSYNKLSSLEGAHTKLGNIKTLNLAGNLLRHLSGLHKLYSLVNLDLSDNRIEQMEEVRSIGSLPCLEHVALLNNPLSIIPDYRTKVLAQFGERASEVCLDNTVTTEKELDTVEVLKAIQKAKEVKSKLSNPEKKVSEDSRLSAAPCVRPSSSPPSAAPTSASLPQPILSNQGIMFVQEDALASSLSSTDSLTPEDRSIVGGCSDSLESIPAGQAPSDDLRDVPGAVGGVSPEHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREEGQGEQGEDDEEEEDVAENRYFEMGPPDTEEEEEGGPGEEEEEEEEEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAAHLHEFHADLRACFAPQHMAMLCSPVLYGSHTSLQEFLRQLLTFYKVAGGCQERSQGCFPVYLVYSDKRMVQTAPGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTPQHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRIYNQLRASLQDLKTVVIAKTPATGGGPQSPLVDGQPAESRASSDQNLQEAPAETTAPALVEAPALTPGEASAPSPAKTPAPVEASVPALVPVEVPEEAEASAEIPVEALAQAEAPAQYPSEPLIRSMSEDNQIPSHLPACPSLRHIASLRGRAVVEFFHSSIAEVENEELRHLLWSSVVFYQMPGLEVTACVLLSTKAVYFVLHDGLRRYFSEPLQDFWHQKNTDYNNSPFHISQCFVLKLSDLQVVNVGLFDQYFRLTGSCPAQVVTCLTRDSYLTHCFLQHLMAVLSLLERTPSPEPVDKDFYSEFGNKTTGKMENYELIHSSRVKFTYPSEEEVGDLTFTVAQKMAAPEKAPALSILLCVQAFQVGSPQPGRCRGLLRPKTLLLTSAEIFLLDEDFVHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYQTYPQALTLVFDDVQGHDLMGSVTLDHFGEVPGGPARGGQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

Target ORF information:

RefSeq Version	XM_002697064.4
Organism	Bos taurus(cattle)
Definition	Bos taurus nischarin (NISCH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697064.4

ORF Insert Sequence:

ATGCCTGCCA GATCCAGTGC TAGGGCCATG GAGGAGGCTA AAAGGGGCCC CCTGCTTCTC 
AGCAAGGGTC AGGTTTACAT CATCCAGATC ACTGATGGGA GCCATGAGTG GACAGTCAAG 
CACCGCTACA GTGATTTCCA CGACCTACAT GAAAAGCTTG TTGCAGAGAA GAAGATTGAT 
AGAAGTCTGC TTCCGCCCAA AAAGATAATT GGGAAAAACT CCAGGAGCTT GGTGGAAAAG 
AGGGAGAAGG ATCTAGAAAT TTATCTCCAG ACGCTCCTGG CCACCTTCCC TGATGTGGCC 
CCCAGAGTGC TGGCCCAGTT CTTGCATTTC CACTTTTACG AGATTAATGG CATCACTGCA 
GCACTGGCTG AGGAACTCTT CGAAAAAGGA GAACAGCTCC TGGGGGCTGG CGAGGTCTTC 
GCCATTGGCC CCCTGCAGCT CTACGCAGTC ACCGAGCAGC TGCAGCAGGG AAAGCCCACG 
TGCGCCAGCG GGGATGCCAA GACTGACCTG GGGCACATCC TGGACTTCAC CTGTCGCCTT 
AAGTACCTTA AGGTTTCTGG CACAGAAGGA CCTTTTGGGA CCAGCAACAT TCAGGAGCAG 
CTTCTGCCTT TTGATCTGTC TATATTCAAG TCTCTTCATC AGGTGGAGAT AAGTCACTGT 
GATGCCAGGC GCATCCGGGG GCTGGTTGCA TCCAAGCCCA CCTTGGCCAC AATGAGCGTC 
CGTTTCTCAG CAACCTCCAT GAAGGAAGTC CTCGTTCCCG AAGCCTCGGA GTTCGATGAG 
TGGGAGCCAG CAGGCGCAGC CCTGGAGGGC CCCGTGACTG CCGTCATCCC CACATGGCAG 
GCTCTGACCG CTCTGGACTT GAGTCACAAT AGCATCTCTG AGATCGATGA TTCTGTGAAA 
CTGATTCCAA AGATTGAGTT CCTGGACCTG AGTCACAATG GAGTGCTGGT CATGAACAAT 
TTGCAGCACC TGTACAACCT TGTGCACGTG GACCTGTCGT ACAACAAGCT CTCCTCCTTG 
GAAGGGGCTC ACACCAAACT GGGCAACATC AAGACCCTGA ACCTGGCGGG CAACCTCCTG 
AGGCATCTGA GTGGCCTGCA TAAGCTCTAT TCCCTGGTCA ACCTGGACCT CAGCGACAAC 
AGAATCGAAC AGATGGAAGA GGTCAGAAGC ATCGGCAGCC TCCCATGTCT GGAGCACGTG 
GCTCTGCTGA ACAACCCCCT GAGTATCATC CCCGACTATC GGACCAAGGT GCTGGCTCAG 
TTCGGAGAGC GGGCCTCAGA GGTCTGCCTG GACAACACAG TGACCACAGA GAAAGAGTTG 
GACACAGTGG AAGTGCTAAA AGCAATTCAG AAAGCCAAAG AGGTCAAGTC CAAATTAAGC 
AACCCTGAGA AGAAGGTCAG TGAGGATTCT CGGCTCTCTG CTGCCCCCTG TGTCAGACCC 
AGCAGCTCCC CACCCAGCGC GGCTCCCACC TCTGCCTCCC TGCCCCAGCC CATCCTCTCC 
AACCAAGGAA TCATGTTCGT GCAAGAGGAT GCCCTGGCCA GCAGCCTCTC ATCCACCGAC 
AGTCTGACTC CTGAGGACCG GTCCATTGTG GGAGGATGCT CTGATTCCTT GGAGTCCATC 
CCTGCGGGAC AGGCACCTTC TGATGATTTA AGGGACGTGC CGGGAGCTGT TGGCGGTGTG 
AGCCCCGAGC ACGCGGAGCC AGAGGTCCAG GTGGTGCCTG GGTCCGGCCA GATCATCTTC 
CTGCCCTTTA CCTGCATTGG CTACACTGCC ACCAACCAGG ACTTCATCCA GCGCCTGAGC 
ACGCTCATCC GACAGGCTAT CGAGCGGCAG CTCCCTGCTT GGATTGAGGC CGCCAACCAG 
CGAGAGGAGG GCCAGGGGGA GCAGGGCGAG GATGATGAGG AGGAGGAGGA CGTTGCTGAG 
AATCGCTACT TCGAAATGGG GCCCCCAGAT ACAGAGGAAG AGGAGGAGGG TGGTCCAGGA 
GAGGAAGAGG AGGAAGAGGA GGAGGAGGAG GCTGAGGAGG AGCGCCTGGC TCTGGAGTGG 
GCGCTGGGCG CAGACGAGGA CTTCCTGCTG GAGCACATCC GCATCCTCAA GGTGCTCTGG 
TGCTTCCTGA TCCACGTGCA GGGCAGCATC CGCCAGTTCG CCGCCTGCCT CGTACTCACC 
GACTTCGGCA TCGCTGTCTT CGAGATCCCG CACCAGGAGT CGCGGGGCAG CAGCCAGCAC 
ATCCTCTCCT CGCTGCGCTT CGTGTTCTGC TTCCCGCACG GCGACCTCAC CGAGTTCGGC 
TTCCTCATGC CGGAGCTGTG CCTGGTGCTG AAGGTGCGGC ACAGTGAGAA CACGCTCTTC 
ATCATCTCCG ACGCTGCCCA CCTGCACGAG TTCCATGCCG ACCTGCGCGC CTGCTTCGCC 
CCGCAGCACA TGGCCATGCT GTGCAGCCCC GTGCTTTACG GCAGCCACAC CAGCCTGCAG 
GAGTTCCTGC GCCAGCTGCT CACCTTCTAC AAAGTGGCAG GCGGCTGCCA GGAGCGCAGC 
CAGGGCTGCT TTCCCGTCTA CCTGGTCTAC AGTGACAAGC GCATGGTGCA GACGGCCCCT 
GGGGACTACT CGGGCAACAT CGAGTGGGCC AGCTGCACCC TCTGCTCGGC CGTGCGGCGC 
TCCTGCTGCG CGCCCTCCGA GGCCGTTAAG TCCGCCGCCA TCCCCTACTG GCTGCTGCTC 
ACGCCCCAGC ACCTCAACGT CATCAAGGCC GACTTCAACC CCATGCCCAA CCGGGGCACC 
CACAACTGCC GCAACCGCAA CAGCTTCAAG CTCAGCCGTG TGCCGCTGTC CACTGTGCTG 
CTGGACCCCA CGCGCAGCTG CACCCAGCCA CGGGGTGCCT TCGCGGACGG CCACGTGCTC 
GAGCTGCTCG TGGGCTACCG CTTCGTCACC GCCATCTTTG TGCTGCCCCA TGAGAAGTTC 
CACTTCCTGC GCATCTACAA CCAGCTGCGG GCCTCGCTGC AGGACCTGAA GACCGTGGTC 
ATCGCCAAGA CCCCCGCAAC GGGGGGCGGG CCTCAGAGCC CCCTCGTGGA CGGCCAGCCT 
GCAGAGAGCA GGGCCAGCAG TGACCAGAAT CTCCAAGAAG CTCCAGCAGA GACTACAGCC 
CCGGCCCTGG TGGAGGCCCC TGCTCTTACT CCAGGAGAGG CCTCAGCCCC AAGCCCAGCA 
AAGACCCCAG CTCCAGTGGA GGCCTCAGTG CCAGCTCTTG TTCCAGTGGA GGTCCCCGAG 
GAGGCCGAGG CCTCAGCAGA GATCCCAGTG GAGGCTCTGG CCCAAGCAGA GGCCCCTGCA 
CAGTACCCAA GTGAGCCCCT GATCCGGTCC ATGTCGGAGG ACAATCAGAT CCCGTCACAC 
CTGCCTGCCT GCCCGTCTCT CCGGCACATC GCCAGCCTGC GTGGACGGGC CGTGGTCGAG 
TTCTTCCACA GCAGCATTGC TGAGGTGGAA AATGAGGAGC TGCGGCACCT CCTGTGGTCA 
TCGGTGGTGT TCTACCAGAT GCCGGGGCTG GAGGTGACAG CCTGTGTGCT GCTCTCTACC 
AAGGCCGTGT ACTTCGTGCT GCACGACGGC CTCCGCCGCT ACTTCTCGGA GCCGCTGCAG 
GATTTCTGGC ATCAGAAAAA CACCGACTAC AACAACAGCC CCTTCCACAT CTCTCAGTGC 
TTTGTTTTGA AACTCAGCGA CCTGCAGGTA GTCAACGTTG GGCTGTTTGA CCAGTATTTC 
CGGCTAACAG GCTCCTGCCC GGCGCAGGTG GTTACCTGCT TGACGCGGGA CAGCTACCTG 
ACCCACTGCT TCCTCCAGCA CCTCATGGCT GTGCTCTCCT TGCTGGAACG CACACCCTCA 
CCTGAGCCTG TGGACAAAGA CTTCTATTCT GAGTTTGGAA ACAAGACCAC AGGGAAGATG 
GAAAACTATG AGCTGATCCA CTCGAGCCGC GTCAAGTTCA CCTACCCCAG CGAGGAGGAG 
GTCGGAGACC TGACGTTCAC CGTGGCCCAG AAGATGGCTG CGCCGGAGAA GGCGCCAGCC 
CTCAGCATCC TGCTGTGTGT GCAGGCCTTC CAGGTGGGCT CGCCGCAGCC GGGGCGCTGC 
AGGGGCTTGC TGCGCCCCAA GACGCTCCTG CTCACCAGCG CCGAGATCTT CCTCCTGGAC 
GAGGACTTCG TCCACTACCC GCTGCCCGAG TTCGCCAAAG AGCCGCCACA GAGGGACCGG 
TACCGGCTGG ATGACGGGCG CCGCGTCCGG GACCTGGACC GCGTGCTCAT GGGCTACCAG 
ACATACCCAC AGGCCCTCAC CCTGGTCTTC GATGATGTGC AGGGCCACGA CCTCATGGGC 
AGCGTCACCC TGGACCACTT CGGGGAGGTG CCAGGTGGCC CGGCTAGGGG TGGCCAGGGC 
CGTGAGGTCC AGTGGCAGGT GTTTGTCCCC AGCGCGGAGA GCCGAGAGAA GCTCATCTCG 
CTGCTGGCCC GTCAGTGGGA GGCCCTGTGT GGCCGGGAGC TGCCCGTGGA GCTCACCGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NISCH ( XM_015468771.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324257.1 Bos taurus nischarin (NISCH), transcript variant X3, mRNA.

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CloneID	OBa95138
Clone ID Related Accession (Same CDS sequence)	XM_015468771.1
Accession Version	XM_015468771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nischarin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGTGC AAGAGGATGC CCTGGCCAGC AGCCTCTCAT CCACCGACAG TCTGACTCCT 
GAGGACCGGT CCATTGTGGG AGGATGCTCT GATTCCTTGG AGTCCATCCC TGCGGGACAG 
GCACCTTCTG ATGATTTAAG GGACGTGCCG GGAGCTGTTG GCGGTGTGAG CCCCGAGCAC 
GCGGAGCCAG AGGTCCAGGT GGTGCCTGGG TCCGGCCAGA TCATCTTCCT GCCCTTTACC 
TGCATTGGCT ACACTGCCAC CAACCAGGAC TTCATCCAGC GCCTGAGCAC GCTCATCCGA 
CAGGCTATCG AGCGGCAGCT CCCTGCTTGG ATTGAGGCCG CCAACCAGCG AGAGGAGGGC 
CAGGGGGAGC AGGGCGAGGA TGATGAGGAG GAGGAGGACG TTGCTGAGAA TCGCTACTTC 
GAAATGGGGC CCCCAGATAC AGAGGAAGAG GAGGAGGGTG GTCCAGGAGA GGAAGAGGAG 
GAAGAGGAGG AGGAGGAGGC TGAGGAGGAG CGCCTGGCTC TGGAGTGGGC GCTGGGCGCA 
GACGAGGACT TCCTGCTGGA GCACATCCGC ATCCTCAAGG TGCTCTGGTG CTTCCTGATC 
CACGTGCAGG GCAGCATCCG CCAGTTCGCC GCCTGCCTCG TACTCACCGA CTTCGGCATC 
GCTGTCTTCG AGATCCCGCA CCAGGAGTCG CGGGGCAGCA GCCAGCACAT CCTCTCCTCG 
CTGCGCTTCG TGTTCTGCTT CCCGCACGGC GACCTCACCG AGTTCGGCTT CCTCATGCCG 
GAGCTGTGCC TGGTGCTGAA GGTGCGGCAC AGTGAGAACA CGCTCTTCAT CATCTCCGAC 
GCTGCCCACC TGCACGAGTT CCATGCCGAC CTGCGCGCCT GCTTCGCCCC GCAGCACATG 
GCCATGCTGT GCAGCCCCGT GCTTTACGGC AGCCACACCA GCCTGCAGGA GTTCCTGCGC 
CAGCTGCTCA CCTTCTACAA AGTGGCAGGC GGCTGCCAGG AGCGCAGCCA GGGCTGCTTT 
CCCGTCTACC TGGTCTACAG TGACAAGCGC ATGGTGCAGA CGGCCCCTGG GGACTACTCG 
GGCAACATCG AGTGGGCCAG CTGCACCCTC TGCTCGGCCG TGCGGCGCTC CTGCTGCGCG 
CCCTCCGAGG CCGTTAAGTC CGCCGCCATC CCCTACTGGC TGCTGCTCAC GCCCCAGCAC 
CTCAACGTCA TCAAGGCCGA CTTCAACCCC ATGCCCAACC GGGGCACCCA CAACTGCCGC 
AACCGCAACA GCTTCAAGCT CAGCCGTGTG CCGCTGTCCA CTGTGCTGCT GGACCCCACG 
CGCAGCTGCA CCCAGCCACG GGGTGCCTTC GCGGACGGCC ACGTGCTCGA GCTGCTCGTG 
GGCTACCGCT TCGTCACCGC CATCTTTGTG CTGCCCCATG AGAAGTTCCA CTTCCTGCGC 
ATCTACAACC AGCTGCGGGC CTCGCTGCAG GACCTGAAGA CCGTGGTCAT CGCCAAGACC 
CCCGCAACGG GGGGCGGGCC TCAGAGCCCC CTCGTGGACG GCCAGCCTGC AGAGAGCAGG 
GCCAGCAGTG ACCAGAATCT CCAAGAAGCT CCAGCAGAGA CTACAGCCCC GGCCCTGGTG 
GAGGCCCCTG CTCTTACTCC AGGAGAGGCC TCAGCCCCAA GCCCAGCAAA GACCCCAGCT 
CCAGTGGAGG CCTCAGTGCC AGCTCTTGTT CCAGTGGAGG TCCCCGAGGA GGCCGAGGCC 
TCAGCAGAGA TCCCAGTGGA GGCTCTGGCC CAAGCAGAGG CCCCTGCACA GTACCCAAGT 
GAGCCCCTGA TCCGGTCCAT GTCGGAGGAC AATCAGATCC CGTCACACCT GCCTGCCTGC 
CCGTCTCTCC GGCACATCGC CAGCCTGCGT GGACGGGCCG TGGTCGAGTT CTTCCACAGC 
AGCATTGCTG AGGTGGAAAA TGAGGAGCTG CGGCACCTCC TGTGGTCATC GGTGGTGTTC 
TACCAGATGC CGGGGCTGGA GGTGACAGCC TGTGTGCTGC TCTCTACCAA GGCCGTGTAC 
TTCGTGCTGC ACGACGGCCT CCGCCGCTAC TTCTCGGAGC CGCTGCAGGA TTTCTGGCAT 
CAGAAAAACA CCGACTACAA CAACAGCCCC TTCCACATCT CTCAGTGCTT TGTTTTGAAA 
CTCAGCGACC TGCAGGTAGT CAACGTTGGG CTGTTTGACC AGTATTTCCG GCTAACAGGC 
TCCTGCCCGG CGCAGGTGGT TACCTGCTTG ACGCGGGACA GCTACCTGAC CCACTGCTTC 
CTCCAGCACC TCATGGCTGT GCTCTCCTTG CTGGAACGCA CACCCTCACC TGAGCCTGTG 
GACAAAGACT TCTATTCTGA GTTTGGAAAC AAGACCACAG GGAAGATGGA AAACTATGAG 
CTGATCCACT CGAGCCGCGT CAAGTTCACC TACCCCAGCG AGGAGGAGGT CGGAGACCTG 
ACGTTCACCG TGGCCCAGAA GATGGCTGCG CCGGAGAAGG CGCCAGCCCT CAGCATCCTG 
CTGTGTGTGC AGGCCTTCCA GGTGGGCTCG CCGCAGCCGG GGCGCTGCAG GGGCTTGCTG 
CGCCCCAAGA CGCTCCTGCT CACCAGCGCC GAGATCTTCC TCCTGGACGA GGACTTCGTC 
CACTACCCGC TGCCCGAGTT CGCCAAAGAG CCGCCACAGA GGGACCGGTA CCGGCTGGAT 
GACGGGCGCC GCGTCCGGGA CCTGGACCGC GTGCTCATGG GCTACCAGAC ATACCCACAG 
GCCCTCACCC TGGTCTTCGA TGATGTGCAG GGCCACGACC TCATGGGCAG CGTCACCCTG 
GACCACTTCG GGGAGGTGCC AGGTGGCCCG GCTAGGGGTG GCCAGGGCCG TGAGGTCCAG 
TGGCAGGTGT TTGTCCCCAG CGCGGAGAGC CGAGAGAAGC TCATCTCGCT GCTGGCCCGT 
CAGTGGGAGG CCCTGTGTGG CCGGGAGCTG CCCGTGGAGC TCACCGGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324257.1
CDS	2361..5351
Translation	MFVQEDALASSLSSTDSLTPEDRSIVGGCSDSLESIPAGQAPSDDLRDVPGAVGGVSPEHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREEGQGEQGEDDEEEEDVAENRYFEMGPPDTEEEEEGGPGEEEEEEEEEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAAHLHEFHADLRACFAPQHMAMLCSPVLYGSHTSLQEFLRQLLTFYKVAGGCQERSQGCFPVYLVYSDKRMVQTAPGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTPQHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRIYNQLRASLQDLKTVVIAKTPATGGGPQSPLVDGQPAESRASSDQNLQEAPAETTAPALVEAPALTPGEASAPSPAKTPAPVEASVPALVPVEVPEEAEASAEIPVEALAQAEAPAQYPSEPLIRSMSEDNQIPSHLPACPSLRHIASLRGRAVVEFFHSSIAEVENEELRHLLWSSVVFYQMPGLEVTACVLLSTKAVYFVLHDGLRRYFSEPLQDFWHQKNTDYNNSPFHISQCFVLKLSDLQVVNVGLFDQYFRLTGSCPAQVVTCLTRDSYLTHCFLQHLMAVLSLLERTPSPEPVDKDFYSEFGNKTTGKMENYELIHSSRVKFTYPSEEEVGDLTFTVAQKMAAPEKAPALSILLCVQAFQVGSPQPGRCRGLLRPKTLLLTSAEIFLLDEDFVHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYQTYPQALTLVFDDVQGHDLMGSVTLDHFGEVPGGPARGGQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

Target ORF information:

RefSeq Version	XM_015468771.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nischarin (NISCH), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468771.1

ORF Insert Sequence:

ATGTTCGTGC AAGAGGATGC CCTGGCCAGC AGCCTCTCAT CCACCGACAG TCTGACTCCT 
GAGGACCGGT CCATTGTGGG AGGATGCTCT GATTCCTTGG AGTCCATCCC TGCGGGACAG 
GCACCTTCTG ATGATTTAAG GGACGTGCCG GGAGCTGTTG GCGGTGTGAG CCCCGAGCAC 
GCGGAGCCAG AGGTCCAGGT GGTGCCTGGG TCCGGCCAGA TCATCTTCCT GCCCTTTACC 
TGCATTGGCT ACACTGCCAC CAACCAGGAC TTCATCCAGC GCCTGAGCAC GCTCATCCGA 
CAGGCTATCG AGCGGCAGCT CCCTGCTTGG ATTGAGGCCG CCAACCAGCG AGAGGAGGGC 
CAGGGGGAGC AGGGCGAGGA TGATGAGGAG GAGGAGGACG TTGCTGAGAA TCGCTACTTC 
GAAATGGGGC CCCCAGATAC AGAGGAAGAG GAGGAGGGTG GTCCAGGAGA GGAAGAGGAG 
GAAGAGGAGG AGGAGGAGGC TGAGGAGGAG CGCCTGGCTC TGGAGTGGGC GCTGGGCGCA 
GACGAGGACT TCCTGCTGGA GCACATCCGC ATCCTCAAGG TGCTCTGGTG CTTCCTGATC 
CACGTGCAGG GCAGCATCCG CCAGTTCGCC GCCTGCCTCG TACTCACCGA CTTCGGCATC 
GCTGTCTTCG AGATCCCGCA CCAGGAGTCG CGGGGCAGCA GCCAGCACAT CCTCTCCTCG 
CTGCGCTTCG TGTTCTGCTT CCCGCACGGC GACCTCACCG AGTTCGGCTT CCTCATGCCG 
GAGCTGTGCC TGGTGCTGAA GGTGCGGCAC AGTGAGAACA CGCTCTTCAT CATCTCCGAC 
GCTGCCCACC TGCACGAGTT CCATGCCGAC CTGCGCGCCT GCTTCGCCCC GCAGCACATG 
GCCATGCTGT GCAGCCCCGT GCTTTACGGC AGCCACACCA GCCTGCAGGA GTTCCTGCGC 
CAGCTGCTCA CCTTCTACAA AGTGGCAGGC GGCTGCCAGG AGCGCAGCCA GGGCTGCTTT 
CCCGTCTACC TGGTCTACAG TGACAAGCGC ATGGTGCAGA CGGCCCCTGG GGACTACTCG 
GGCAACATCG AGTGGGCCAG CTGCACCCTC TGCTCGGCCG TGCGGCGCTC CTGCTGCGCG 
CCCTCCGAGG CCGTTAAGTC CGCCGCCATC CCCTACTGGC TGCTGCTCAC GCCCCAGCAC 
CTCAACGTCA TCAAGGCCGA CTTCAACCCC ATGCCCAACC GGGGCACCCA CAACTGCCGC 
AACCGCAACA GCTTCAAGCT CAGCCGTGTG CCGCTGTCCA CTGTGCTGCT GGACCCCACG 
CGCAGCTGCA CCCAGCCACG GGGTGCCTTC GCGGACGGCC ACGTGCTCGA GCTGCTCGTG 
GGCTACCGCT TCGTCACCGC CATCTTTGTG CTGCCCCATG AGAAGTTCCA CTTCCTGCGC 
ATCTACAACC AGCTGCGGGC CTCGCTGCAG GACCTGAAGA CCGTGGTCAT CGCCAAGACC 
CCCGCAACGG GGGGCGGGCC TCAGAGCCCC CTCGTGGACG GCCAGCCTGC AGAGAGCAGG 
GCCAGCAGTG ACCAGAATCT CCAAGAAGCT CCAGCAGAGA CTACAGCCCC GGCCCTGGTG 
GAGGCCCCTG CTCTTACTCC AGGAGAGGCC TCAGCCCCAA GCCCAGCAAA GACCCCAGCT 
CCAGTGGAGG CCTCAGTGCC AGCTCTTGTT CCAGTGGAGG TCCCCGAGGA GGCCGAGGCC 
TCAGCAGAGA TCCCAGTGGA GGCTCTGGCC CAAGCAGAGG CCCCTGCACA GTACCCAAGT 
GAGCCCCTGA TCCGGTCCAT GTCGGAGGAC AATCAGATCC CGTCACACCT GCCTGCCTGC 
CCGTCTCTCC GGCACATCGC CAGCCTGCGT GGACGGGCCG TGGTCGAGTT CTTCCACAGC 
AGCATTGCTG AGGTGGAAAA TGAGGAGCTG CGGCACCTCC TGTGGTCATC GGTGGTGTTC 
TACCAGATGC CGGGGCTGGA GGTGACAGCC TGTGTGCTGC TCTCTACCAA GGCCGTGTAC 
TTCGTGCTGC ACGACGGCCT CCGCCGCTAC TTCTCGGAGC CGCTGCAGGA TTTCTGGCAT 
CAGAAAAACA CCGACTACAA CAACAGCCCC TTCCACATCT CTCAGTGCTT TGTTTTGAAA 
CTCAGCGACC TGCAGGTAGT CAACGTTGGG CTGTTTGACC AGTATTTCCG GCTAACAGGC 
TCCTGCCCGG CGCAGGTGGT TACCTGCTTG ACGCGGGACA GCTACCTGAC CCACTGCTTC 
CTCCAGCACC TCATGGCTGT GCTCTCCTTG CTGGAACGCA CACCCTCACC TGAGCCTGTG 
GACAAAGACT TCTATTCTGA GTTTGGAAAC AAGACCACAG GGAAGATGGA AAACTATGAG 
CTGATCCACT CGAGCCGCGT CAAGTTCACC TACCCCAGCG AGGAGGAGGT CGGAGACCTG 
ACGTTCACCG TGGCCCAGAA GATGGCTGCG CCGGAGAAGG CGCCAGCCCT CAGCATCCTG 
CTGTGTGTGC AGGCCTTCCA GGTGGGCTCG CCGCAGCCGG GGCGCTGCAG GGGCTTGCTG 
CGCCCCAAGA CGCTCCTGCT CACCAGCGCC GAGATCTTCC TCCTGGACGA GGACTTCGTC 
CACTACCCGC TGCCCGAGTT CGCCAAAGAG CCGCCACAGA GGGACCGGTA CCGGCTGGAT 
GACGGGCGCC GCGTCCGGGA CCTGGACCGC GTGCTCATGG GCTACCAGAC ATACCCACAG 
GCCCTCACCC TGGTCTTCGA TGATGTGCAG GGCCACGACC TCATGGGCAG CGTCACCCTG 
GACCACTTCG GGGAGGTGCC AGGTGGCCCG GCTAGGGGTG GCCAGGGCCG TGAGGTCCAG 
TGGCAGGTGT TTGTCCCCAG CGCGGAGAGC CGAGAGAAGC TCATCTCGCT GCTGGCCCGT 
CAGTGGGAGG CCCTGTGTGG CCGGGAGCTG CCCGTGGAGC TCACCGGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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NISCH cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

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