Ywhae cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ywhae
Entrez Gene ID	29753
Full Name	tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon
Synonyms	14-3-3e
General protein information	Preferred Names tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon Names 14-3-3 protein epsilon 14-3-3 epsilon MSF L mitochondrial import stimulation factor (MSF) L subunit mitochondrial import stimulation factor L subunit tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein epsilon polypeptide
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q24
Summary	epsilon subunit of a 14-3-3 protein; mediates signal transduction via calcium/calmodulin-dependent protein kinase II and protein kinase C [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031603.1	NP_113791.1	14-3-3 protein epsilon

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	YWHAE	NP_001233297.1
Saccharomyces cerevisiae (baker's yeast)	BMH1	NP_011104.3
Neurospora crassa	NCU02806	XP_964462.1
Arabidopsis thaliana (thale cress)	GRF3	NP_568557.1
Oryza sativa (rice)	Os04g0462500	NP_001053003.1
Oryza sativa (rice)	Os08g0430500	NP_001061856.1
Mus musculus (house mouse)	Ywhae	NP_033562.3
Arabidopsis thaliana (thale cress)	GRF5	NP_568325.1
Xenopus tropicalis (tropical clawed frog)	ywhae	NP_001008156.1
Homo sapiens (human)	YWHAE	NP_006752.1
Bos taurus (cattle)	YWHAE	NP_776916.1
Drosophila melanogaster (fruit fly)	14-3-3epsilon	NP_732311.1
Schizosaccharomyces pombe (fission yeast)	rad25	NP_594247.1
Danio rerio (zebrafish)	ywhae2	NP_001013359.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001151	XP_322009.2
Saccharomyces cerevisiae (baker's yeast)	BMH2	NP_010384.3
Eremothecium gossypii	AGOS_AGR107C	NP_986773.1
Arabidopsis thaliana (thale cress)	GRF7	NP_566174.1
Oryza sativa (rice)	Os02g0580300	NP_001047234.1
Canis lupus familiaris (dog)	YWHAE	XP_854358.1
Rattus norvegicus (Norway rat)	Ywhae	NP_113791.1
Gallus gallus (chicken)	YWHAE	NP_001006219.1
Kluyveromyces lactis	KLLA0F12100g	XP_455629.1
Magnaporthe oryzae (rice blast fungus)	MGG_01588	XP_003714567.1
Oryza sativa (rice)	Os03g0710800	NP_001051051.1

Gene Ontology
Bibliography

Related articles in PubMed

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

14-3-3 Proteins in the regulation of rotenone-induced neurotoxicity might be via its isoform 14-3-3epsilon's involvement in autophagy.
Sai Y, Peng K, Ye F, Zhao X, Zhao Y, Zou Z, Cao J, Dong Z
Cellular and molecular neurobiology33(8)1109-21(2013 Nov)

The role of 14-3-3? interaction with phosphorylated Cdc25B at its Ser321 in the release of the mouse oocyte from prophase I arrest.
Meng J, Cui C, Liu Y, Jin M, Wu D, Liu C, Wang E, Yu B
PloS one8(1)e53633(2013)

Homocysteine induces apoptosis of rat hippocampal neurons by inhibiting 14-3-3? expression and activating calcineurin.
Wang J, Bai X, Chen Y, Zhao Y, Liu X
PloS one7(11)e48247(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The regulation of 14-3-3 proteins in rotenone-induced neurotoxicity might be associated with its isoform 14-3-3epsilon's involvement in autophagy.
Title: 14-3-3 Proteins in the regulation of rotenone-induced neurotoxicity might be via its isoform 14-3-3epsilon's involvement in autophagy.

Homocysteine inhibits expression of 14-3-3epsilon and activates calcineurin in rat hippocampal neurons.
Title: Homocysteine induces apoptosis of rat hippocampal neurons by inhibiting 14-3-3? expression and activating calcineurin.

14-3-3varepsilon binds to Ser12-phosphorylated Fbx4 to mediate dimerization and function.
Title: Phosphorylation-dependent regulation of SCF(Fbx4) dimerization and activity involves a novel component, 14-3-3?.

Ligand-activated PPAR-gamma confers resistance to neuronal apoptosis and cerebral infarction by driving 14-3-3epsilon transcription.
Title: Ligand-activated peroxisome proliferator-activated receptor-gamma protects against ischemic cerebral infarction and neuronal apoptosis by 14-3-3 epsilon upregulation.

the homeostatic level of Syn G in RGC-5 cells is required for transcriptional regulation of protein kinases and phosphatases, controlling phosphorylation of Bad and 14-3-3
Title: Effect of gamma-synuclein silencing on apoptotic pathways in retinal ganglion cells.

The following Ywhae gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ywhae cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08232	NM_031603.1 Latest version!	Rattus norvegicus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (Ywhae), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ywhae ( NM_031603.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113791.1 Rattus norvegicus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (Ywhae), mRNA.

Price & Availability↑

CloneID	ORa08232
Clone ID Related Accession (Same CDS sequence)	NM_031603.1
Accession Version	NM_031603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	14-3-3 protein epsilon
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U53882.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U53882.1, FQ220393.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGATGATC GGGAGGATCT GGTGTACCAG GCGAAGCTGG CAGAGCAGGC CGAGCGATAC 
GACGAAATGG TGGAATCAAT GAAGAAAGTA GCAGGAATGG ACGTGGAGCT GACAGTTGAA 
GAACGAAACC TTTTATCTGT TGCATATAAA AATGTGATTG GAGCCAGAAG AGCATCCTGG 
AGAATAATCA GCAGCATTGA ACAGAAGGAA GAAAACACGG GAGGAGAGGA CAAATTAAAG 
ATGATTCGGG AGTACCGGCA AATGGTTGAA ACTGAGCTCA AGTTAATCTG TTGTGACATT 
CTGGATGTAC TGGACAAGCA CCTCATTCCA GCAGCTAACA CTGGCGAGTC CAAGGTTTCC 
TATTATTACA TGAAAGGGGA CTACCACAGG TATCTGGCTG AGTTTGCCAC AGGAAACGAC 
AGGAAGGAGG CAGCAGAGAA CAGCCTTGTG GCTTACAAAG CTGCTAGTGA CATTGCGATG 
ACAGAACTTC CTCCAACGCA CCCCATTCGT TTAGGTCTTG CTCTCAACTT TTCCGTATTC 
TACTATGAAA TTCTTAATTC CCCCGACCGT GCCTGCAGGT TGGCAAAAGC AGCTTTTGAT 
GACGCAATTG CAGAACTGGA CACGCTGAGT GAAGAAAGCT ATAAGGACTC CACACTCATC 
ATGCAGCTGC TACGTGATAA CCTGACGCTG TGGACCTCAG ACATGCAGGG CGATGGTGAA 
GAGCAGAATA AAGAAGCGCT GCAGGATGTG GAAGATGAGA ATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113791.1
CDS	91..858
Translation	MDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENTGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVSYYYMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVEDENQ

Target ORF information:

RefSeq Version	NM_031603.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (Ywhae), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031603.1

ORF Insert Sequence:

ATGGATGATC GGGAGGATCT GGTGTACCAG GCGAAGCTGG CAGAGCAGGC CGAGCGATAC 
GACGAAATGG TGGAATCAAT GAAGAAAGTA GCAGGAATGG ACGTGGAGCT GACAGTTGAA 
GAACGAAACC TTTTATCTGT TGCATATAAA AATGTGATTG GAGCCAGAAG AGCATCCTGG 
AGAATAATCA GCAGCATTGA ACAGAAGGAA GAAAACACGG GAGGAGAGGA CAAATTAAAG 
ATGATTCGGG AGTACCGGCA AATGGTTGAA ACTGAGCTCA AGTTAATCTG TTGTGACATT 
CTGGATGTAC TGGACAAGCA CCTCATTCCA GCAGCTAACA CTGGCGAGTC CAAGGTTTCC 
TATTATTACA TGAAAGGGGA CTACCACAGG TATCTGGCTG AGTTTGCCAC AGGAAACGAC 
AGGAAGGAGG CAGCAGAGAA CAGCCTTGTG GCTTACAAAG CTGCTAGTGA CATTGCGATG 
ACAGAACTTC CTCCAACGCA CCCCATTCGT TTAGGTCTTG CTCTCAACTT TTCCGTATTC 
TACTATGAAA TTCTTAATTC CCCCGACCGT GCCTGCAGGT TGGCAAAAGC AGCTTTTGAT 
GACGCAATTG CAGAACTGGA CACGCTGAGT GAAGAAAGCT ATAAGGACTC CACACTCATC 
ATGCAGCTGC TACGTGATAA CCTGACGCTG TGGACCTCAG ACATGCAGGG CGATGGTGAA 
GAGCAGAATA AAGAAGCGCT GCAGGATGTG GAAGATGAGA ATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

14-3-3 Proteins in the regulation of rotenone-induced neurotoxicity might be via its isoform 14-3-3epsilon's involvement in autophagy.
Cellular and molecular neurobiology33(8)1109-21(2013 Nov)
Sai Y,Peng K,Ye F,Zhao X,Zhao Y,Zou Z,Cao J,Dong Z

The role of 14-3-3? interaction with phosphorylated Cdc25B at its Ser321 in the release of the mouse oocyte from prophase I arrest.
PloS one8(1)e53633(2013)
Meng J,Cui C,Liu Y,Jin M,Wu D,Liu C,Wang E,Yu B

Homocysteine induces apoptosis of rat hippocampal neurons by inhibiting 14-3-3? expression and activating calcineurin.
PloS one7(11)e48247(2012)
Wang J,Bai X,Chen Y,Zhao Y,Liu X