Ywhae cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ywhae
Entrez Gene ID	22627
Full Name	tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
Synonyms	AU019196
General protein information	Preferred Names tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide Names 14-3-3 protein epsilon 14-3-3 epsilon 14-3-3E tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein, epsilon polypeptide
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B5\|11 45.92 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009536.4	NP_033562.3	14-3-3 protein epsilon

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	YWHAE	NP_001233297.1
Saccharomyces cerevisiae (baker's yeast)	BMH1	NP_011104.3
Neurospora crassa	NCU02806	XP_964462.1
Arabidopsis thaliana (thale cress)	GRF3	NP_568557.1
Oryza sativa (rice)	Os04g0462500	NP_001053003.1
Oryza sativa (rice)	Os08g0430500	NP_001061856.1
Mus musculus (house mouse)	Ywhae	NP_033562.3
Arabidopsis thaliana (thale cress)	GRF5	NP_568325.1
Xenopus tropicalis (tropical clawed frog)	ywhae	NP_001008156.1
Homo sapiens (human)	YWHAE	NP_006752.1
Bos taurus (cattle)	YWHAE	NP_776916.1
Drosophila melanogaster (fruit fly)	14-3-3epsilon	NP_732311.1
Schizosaccharomyces pombe (fission yeast)	rad25	NP_594247.1
Danio rerio (zebrafish)	ywhae2	NP_001013359.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001151	XP_322009.2
Saccharomyces cerevisiae (baker's yeast)	BMH2	NP_010384.3
Eremothecium gossypii	AGOS_AGR107C	NP_986773.1
Arabidopsis thaliana (thale cress)	GRF7	NP_566174.1
Oryza sativa (rice)	Os02g0580300	NP_001047234.1
Canis lupus familiaris (dog)	YWHAE	XP_854358.1
Rattus norvegicus (Norway rat)	Ywhae	NP_113791.1
Gallus gallus (chicken)	YWHAE	NP_001006219.1
Kluyveromyces lactis	KLLA0F12100g	XP_455629.1
Magnaporthe oryzae (rice blast fungus)	MGG_01588	XP_003714567.1
Oryza sativa (rice)	Os03g0710800	NP_001051051.1

Gene Ontology
Bibliography

Related articles in PubMed

14-3-3? loss leads to neonatal lethality by microRNA-126 downregulation-mediated developmental defects in lung vasculature.
Yang J, Joshi S, Wang Q, Li P, Wang H, Xiong Y, Xiao Y, Wang J, Parker-Thornburg J, Behringer RR, Yu D
Cell & bioscience758(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The complete ablation of the 14-3-3epsilon protein results in multiple defects in neuropsychiatric behaviors in mice.
Title: Complete ablation of the 14-3-3epsilon protein results in multiple defects in neuropsychiatric behaviors.

The data obtained from the 14-3-3epsilon/14-3-3zeta/Wnt1-Cre mice strongly indicate the importance of 14-3-3 proteins in the development of melanocyte lineages.
Title: Deficiency of 14-3-3? and 14-3-3? by the Wnt1 promoter-driven Cre recombinase results in pigmentation defects.

14-3-3epsilon might directly bind to CD13, which transmits its signal in chondrocytes to induce a catabolic phenotype similar to that observed in osteoarthritis.
Title: The pro-inflammatory cytokine 14-3-3? is a ligand of CD13 in cartilage.

Data indicate that 14-3-3epsilon is required for the mitotic entry in the fertilized mouse eggs and responsible for sequestering the CDC25B in cytoplasm. Its binding to CDC25B-S321 phosphorylated by PKA induces mitotic arrest.
Title: 14-3-3 epsilon prevents G2/M transition of fertilized mouse eggs by binding with CDC25B.

Ser321 of Cdc25B is the specific binding site for 14-3-3epsilon binding.
Title: The role of 14-3-3? interaction with phosphorylated Cdc25B at its Ser321 in the release of the mouse oocyte from prophase I arrest.

The following Ywhae gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ywhae cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22557	NM_009536.4 Latest version!	Mus musculus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (Ywhae), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ywhae ( NM_009536.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033562.3 Mus musculus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (Ywhae), mRNA.

Price & Availability↑

CloneID	OMu22557
Clone ID Related Accession (Same CDS sequence)	NM_009536.4
Accession Version	NM_009536.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	14-3-3 protein epsilon
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY293945.1, BC058686.1 and AK135831.1. On Apr 16, 2009 this sequence version replaced NM_009536.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK135831.1, SRR1660817.246990.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGATGATC GGGAGGATCT GGTGTACCAG GCGAAGCTGG CAGAGCAGGC CGAGCGATAC 
GACGAAATGG TGGAATCAAT GAAGAAAGTA GCAGGGATGG ACGTGGAGCT GACAGTTGAA 
GAACGAAACC TTTTATCTGT TGCATATAAA AATGTGATTG GAGCCAGAAG AGCATCCTGG 
AGAATAATCA GCAGCATTGA ACAGAAGGAA GAAAACAAGG GAGGAGAGGA CAAATTAAAG 
ATGATTCGGG AGTACCGGCA AATGGTTGAA ACTGAGCTCA AGTTAATCTG TTGTGACATT 
CTGGATGTAC TGGACAAACA CCTCATTCCA GCAGCTAACA CTGGCGAGTC CAAGGTTTTC 
TATTATAAAA TGAAAGGGGA CTACCACAGG TATCTGGCTG AGTTTGCCAC AGGAAATGAC 
AGGAAGGAGG CAGCAGAGAA CAGCCTCGTG GCTTACAAAG CTGCTAGTGA CATTGCGATG 
ACAGAACTTC CTCCAACGCA CCCCATTCGT TTAGGTCTTG CTCTCAACTT TTCCGTATTC 
TACTATGAAA TTCTTAATTC CCCCGACCGT GCCTGCAGGT TGGCAAAAGC AGCTTTTGAT 
GACGCAATTG CAGAACTGGA CACGCTGAGT GAAGAAAGCT ATAAGGACTC TACGCTCATC 
ATGCAGCTGC TACGTGATAA CCTGACGCTG TGGACCTCAG ACATGCAGGG TGATGGTGAA 
GAGCAGAATA AAGAAGCGCT GCAGGATGTG GAAGATGAGA ATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033562.3
CDS	142..909
Translation	MDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVEDENQ

Target ORF information:

RefSeq Version	NM_009536.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (Ywhae), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009536.4

ORF Insert Sequence:

ATGGATGATC GGGAGGATCT GGTGTACCAG GCGAAGCTGG CAGAGCAGGC CGAGCGATAC 
GACGAAATGG TGGAATCAAT GAAGAAAGTA GCAGGGATGG ACGTGGAGCT GACAGTTGAA 
GAACGAAACC TTTTATCTGT TGCATATAAA AATGTGATTG GAGCCAGAAG AGCATCCTGG 
AGAATAATCA GCAGCATTGA ACAGAAGGAA GAAAACAAGG GAGGAGAGGA CAAATTAAAG 
ATGATTCGGG AGTACCGGCA AATGGTTGAA ACTGAGCTCA AGTTAATCTG TTGTGACATT 
CTGGATGTAC TGGACAAACA CCTCATTCCA GCAGCTAACA CTGGCGAGTC CAAGGTTTTC 
TATTATAAAA TGAAAGGGGA CTACCACAGG TATCTGGCTG AGTTTGCCAC AGGAAATGAC 
AGGAAGGAGG CAGCAGAGAA CAGCCTCGTG GCTTACAAAG CTGCTAGTGA CATTGCGATG 
ACAGAACTTC CTCCAACGCA CCCCATTCGT TTAGGTCTTG CTCTCAACTT TTCCGTATTC 
TACTATGAAA TTCTTAATTC CCCCGACCGT GCCTGCAGGT TGGCAAAAGC AGCTTTTGAT 
GACGCAATTG CAGAACTGGA CACGCTGAGT GAAGAAAGCT ATAAGGACTC TACGCTCATC 
ATGCAGCTGC TACGTGATAA CCTGACGCTG TGGACCTCAG ACATGCAGGG TGATGGTGAA 
GAGCAGAATA AAGAAGCGCT GCAGGATGTG GAAGATGAGA ATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

14-3-3? loss leads to neonatal lethality by microRNA-126 downregulation-mediated developmental defects in lung vasculature.
Cell & bioscience758(2017)
Yang J,Joshi S,Wang Q,Li P,Wang H,Xiong Y,Xiao Y,Wang J,Parker-Thornburg J,Behringer RR,Yu D

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Ywhae cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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