Pcbd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pcbd1
Entrez Gene ID	29700
Full Name	pterin-4 alpha-carbinolamine dehydratase 1
Synonyms	DCoH,Pcbd,Pcd,Phs,TCF1
General protein information	Preferred Names pterin-4 alpha-carbinolamine dehydratase 1 Names pterin-4-alpha-carbinolamine dehydratase 4-alpha-hydroxy-tetrahydropterin dehydratase 6-pyruvoyl-tetrahydropterin synthase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) dimerization cofactor of HNF1 phenylalanine hydroxylase-stimulating protein pterin carbinolamine dehydratase pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20q11
Summary	This gene encodes a member of the pterin-4-alpha-carbinolamine dehydratase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein functions as both a dehydratase involved in tetrahydrobiopterin biosynthesis, and as a cofactor for HNF1A-dependent transcription. [provided by RefSeq, Jan 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001007601.2	NP_001007602.1	pterin-4-alpha-carbinolamine dehydratase
XM_006256414.1	XP_006256476.1	pterin-4-alpha-carbinolamine dehydratase isoform X1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Pcd	NP_477360.2
Pan troglodytes (chimpanzee)	PCBD1	XP_001171466.1
Xenopus tropicalis (tropical clawed frog)	pcbd1	NP_001011016.1
Gallus gallus (chicken)	PCBD1	NP_990236.1
Homo sapiens (human)	PCBD1	NP_000272.1
	PCBD1	XP_001107537.1
Bos taurus (cattle)	PCBD1	NP_001193403.1
Danio rerio (zebrafish)	pcbd1	NP_957108.1
Canis lupus familiaris (dog)	PCBD1	XP_005619043.1
Mus musculus (house mouse)	Pcbd1	NP_079549.1
Rattus norvegicus (Norway rat)	Pcbd1	NP_001007602.1

Gene Ontology
Bibliography

Related articles in PubMed

Next-generation sequencing to generate interactome datasets.
Yu H, Tardivo L, Tam S, Weiner E, Gebreab F, Fan C, Svrzikapa N, Hirozane-Kishikawa T, Rietman E, Yang X, Sahalie J, Salehi-Ashtiani K, Hao T, Cusick ME, Hill DE, Roth FP, Braun P, Vidal M
Nature methods8(6)478-80(2011 Jun)

Kinetic stability may determine the interaction dynamics of the bifunctional protein DCoH1, the dimerization cofactor of the transcription factor HNF-1? .
Rho H, Jones CN, Rose RB
Biochemistry49(47)10187-97(2010 Nov)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

Biochemical and structural basis for partially redundant enzymatic and transcriptional functions of DCoH and DCoH2.
Rose RB, Pullen KE, Bayle JH, Crabtree GR, Alber T
Biochemistry43(23)7345-55(2004 Jun)

Characterization of the chicken and rat DCoH gene domains using an improved ligation-mediated PCR method.
Bossow S, Riepl S, Igo-Kemenes T
Biological chemistry379(11)1359-65(1998 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a point mutation at the DCoH1 tetramer interface, Thr 51 Ser, overcomes the dissociation barrier of the homotetramer and increases the interaction with HNF-1alpha.
Title: Kinetic stability may determine the interaction dynamics of the bifunctional protein DCoH1, the dimerization cofactor of the transcription factor HNF-1? .

Rat Pcbd1 is a moonlighting protein that has dehydratase activity in addition to functioning as a cofactor which regulates HNF1alpha.
Title: Identity of 4a-carbinolamine dehydratase, a component of the phenylalanine hydroxylation system, and DCoH, a transregulator of homeodomain proteins.

The following Pcbd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcbd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa39439	NM_001007601.2 Latest version!	Rattus norvegicus pterin-4 alpha-carbinolamine dehydratase 1 (Pcbd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa39439	XM_006256414.1 Latest version!	Rattus norvegicus pterin-4 alpha-carbinolamine dehydratase 1 (Pcbd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pcbd1 ( NM_001007601.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001007602.1 Rattus norvegicus pterin-4 alpha-carbinolamine dehydratase 1 (Pcbd1), mRNA.

Price & Availability↑

CloneID	ORa39439
Clone ID Related Accession (Same CDS sequence)	NM_001007601.2 , XM_006256414.1
Accession Version	NM_001007601.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	pterin-4-alpha-carbinolamine dehydratase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM060466.1, FM067129.1 and DV724713.1. On Jan 23, 2014 this sequence version replaced NM_001007601.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FM060466.1, FM067129.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 1465414 ##RefSeq-Attributes-END##

ATGGCTGGCA AGGCACACAG GCTGAGTGCT GAGGAACGGG ACCAGCTGCT GCCAAACCTG 
CGGGCTGTGG GGTGGAATGA ACTGGAAGGC CGAGATGCCA TCTTCAAACA GTTCCATTTT 
AAAGACTTCA ACAGGGCTTT TGGCTTCATG ACAAGAGTCG CCCTGCAGGC TGAAAAGCTG 
GACCACCATC CCGAGTGGTT TAACGTGTAC AACAAGGTCC ATATCACCTT GAGCACCCAC 
GAATGTGCCG GTCTTTCTGA ACGGGATATA AACCTGGCCA GCTTCATCGA ACAAGTTGCC 
GTGTCTATGA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007602.1
CDS	39..353
Translation	MAGKAHRLSAEERDQLLPNLRAVGWNELEGRDAIFKQFHFKDFNRAFGFMTRVALQAEKLDHHPEWFNVYNKVHITLSTHECAGLSERDINLASFIEQVAVSMT

Target ORF information:

RefSeq Version	NM_001007601.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pterin-4 alpha-carbinolamine dehydratase 1 (Pcbd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007601.2

ORF Insert Sequence:

ATGGCTGGCA AGGCACACAG GCTGAGTGCT GAGGAACGGG ACCAGCTGCT GCCAAACCTG 
CGGGCTGTGG GGTGGAATGA ACTGGAAGGC CGAGATGCCA TCTTCAAACA GTTCCATTTT 
AAAGACTTCA ACAGGGCTTT TGGCTTCATG ACAAGAGTCG CCCTGCAGGC TGAAAAGCTG 
GACCACCATC CCGAGTGGTT TAACGTGTAC AACAAGGTCC ATATCACCTT GAGCACCCAC 
GAATGTGCCG GTCTTTCTGA ACGGGATATA AACCTGGCCA GCTTCATCGA ACAAGTTGCC 
GTGTCTATGA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcbd1 ( XM_006256414.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256476.1 Rattus norvegicus pterin-4 alpha-carbinolamine dehydratase 1 (Pcbd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa39439
Clone ID Related Accession (Same CDS sequence)	NM_001007601.2 , XM_006256414.1
Accession Version	XM_006256414.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pterin-4-alpha-carbinolamine dehydratase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCA AGGCACACAG GCTGAGTGCT GAGGAACGGG ACCAGCTGCT GCCAAACCTG 
CGGGCTGTGG GGTGGAATGA ACTGGAAGGC CGAGATGCCA TCTTCAAACA GTTCCATTTT 
AAAGACTTCA ACAGGGCTTT TGGCTTCATG ACAAGAGTCG CCCTGCAGGC TGAAAAGCTG 
GACCACCATC CCGAGTGGTT TAACGTGTAC AACAAGGTCC ATATCACCTT GAGCACCCAC 
GAATGTGCCG GTCTTTCTGA ACGGGATATA AACCTGGCCA GCTTCATCGA ACAAGTTGCC 
GTGTCTATGA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256476.1
CDS	297..611
Translation	MAGKAHRLSAEERDQLLPNLRAVGWNELEGRDAIFKQFHFKDFNRAFGFMTRVALQAEKLDHHPEWFNVYNKVHITLSTHECAGLSERDINLASFIEQVAVSMT

Target ORF information:

RefSeq Version	XM_006256414.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pterin-4 alpha-carbinolamine dehydratase 1 (Pcbd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256414.1

ORF Insert Sequence:

ATGGCTGGCA AGGCACACAG GCTGAGTGCT GAGGAACGGG ACCAGCTGCT GCCAAACCTG 
CGGGCTGTGG GGTGGAATGA ACTGGAAGGC CGAGATGCCA TCTTCAAACA GTTCCATTTT 
AAAGACTTCA ACAGGGCTTT TGGCTTCATG ACAAGAGTCG CCCTGCAGGC TGAAAAGCTG 
GACCACCATC CCGAGTGGTT TAACGTGTAC AACAAGGTCC ATATCACCTT GAGCACCCAC 
GAATGTGCCG GTCTTTCTGA ACGGGATATA AACCTGGCCA GCTTCATCGA ACAAGTTGCC 
GTGTCTATGA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Next-generation sequencing to generate interactome datasets.
Nature methods8(6)478-80(2011 Jun)
Yu H,Tardivo L,Tam S,Weiner E,Gebreab F,Fan C,Svrzikapa N,Hirozane-Kishikawa T,Rietman E,Yang X,Sahalie J,Salehi-Ashtiani K,Hao T,Cusick ME,Hill DE,Roth FP,Braun P,Vidal M

Kinetic stability may determine the interaction dynamics of the bifunctional protein DCoH1, the dimerization cofactor of the transcription factor HNF-1? .
Biochemistry49(47)10187-97(2010 Nov)
Rho H,Jones CN,Rose RB

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

Biochemical and structural basis for partially redundant enzymatic and transcriptional functions of DCoH and DCoH2.
Biochemistry43(23)7345-55(2004 Jun)
Rose RB,Pullen KE,Bayle JH,Crabtree GR,Alber T

Characterization of the chicken and rat DCoH gene domains using an improved ligation-mediated PCR method.
Biological chemistry379(11)1359-65(1998 Nov)
Bossow S,Riepl S,Igo-Kemenes T

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Pcbd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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