Pcbd1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pcbd1
Entrez Gene ID	13180
Full Name	pterin 4 alpha carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) 1
Synonyms	Dcoh,Pcbd,Pcd,Phs
General protein information	Preferred Names pterin 4 alpha carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) 1 Names pterin-4-alpha-carbinolamine dehydratase 4-alpha-hydroxy-tetrahydropterin dehydratase 6-pyruvoyl-tetrahydropterin synthase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) dimerization cofactor of HNF1 phenylalanine hydroxylase-stimulating protein pterin carbinolamine dehydratase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 B4\|10 32.14 cM
Summary	This gene encodes a member of the pterin-4-alpha-carbinolamine dehydratase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein functions as both a dehydratase involved in tetrahydrobiopterin biosynthesis, and as a cofactor for HNF1A-dependent transcription. [provided by RefSeq, Apr 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_025273.4	NP_079549.1	pterin-4-alpha-carbinolamine dehydratase

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Pcd	NP_477360.2
Pan troglodytes (chimpanzee)	PCBD1	XP_001171466.1
Xenopus tropicalis (tropical clawed frog)	pcbd1	NP_001011016.1
Gallus gallus (chicken)	PCBD1	NP_990236.1
Homo sapiens (human)	PCBD1	NP_000272.1
	PCBD1	XP_001107537.1
Bos taurus (cattle)	PCBD1	NP_001193403.1
Danio rerio (zebrafish)	pcbd1	NP_957108.1
Canis lupus familiaris (dog)	PCBD1	XP_005619043.1
Mus musculus (house mouse)	Pcbd1	NP_079549.1
Rattus norvegicus (Norway rat)	Pcbd1	NP_001007602.1

Gene Ontology
Bibliography

Related articles in PubMed

?-Cell-Specific Mafk Overexpression Impairs Pancreatic Endocrine Cell Development.
Abdellatif AM, Oishi H, Itagaki T, Jung Y, Shawki HH, Okita Y, Hasegawa Y, Suzuki H, El-Morsy SE, El-Sayed MA, Shoaib MB, Sugiyama F, Takahashi S
PloS one11(2)e0150010(2016)

Recessive mutations in PCBD1 cause a new type of early-onset diabetes.
Simaite D, Kofent J, Gong M, R?schendorf F, Jia S, Arn P, Bentler K, Ellaway C, K?hnen P, Hoffmann GF, Blau N, Spagnoli FM, H?bner N, Raile K
Diabetes63(10)3557-64(2014 Oct)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

An expression profile analysis of ES cell-derived definitive endodermal cells and Pdx1-expressing cells.
Ogaki S, Harada S, Shiraki N, Kume K, Kume S
BMC developmental biology1113(2011 Mar)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HNF1alpha binding kinetics may distinguish regulation by DCoH2, under thermodynamic control, from regulation by DCoH, under kinetic control
Title: Biochemical and structural basis for partially redundant enzymatic and transcriptional functions of DCoH and DCoH2.

Mouse Pcbd1 is a moonlighting protein that has dehydratase activity in addition to functioning as a cofactor which regulates HNF1alpha.
Title: Hyperphenylalaninemia and impaired glucose tolerance in mice lacking the bifunctional DCoH gene.

The following Pcbd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcbd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu23172	NM_025273.4 Latest version!	Mus musculus pterin 4 alpha carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) 1 (Pcbd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pcbd1 ( NM_025273.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079549.1 Mus musculus pterin 4 alpha carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) 1 (Pcbd1), mRNA.

Price & Availability↑

CloneID	OMu23172
Clone ID Related Accession (Same CDS sequence)	NM_025273.4
Accession Version	NM_025273.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	pterin-4-alpha-carbinolamine dehydratase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY116433.1, AK007401.1 and AC153515.8. On Jan 23, 2014 this sequence version replaced NM_025273.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK007401.1, BY707492.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 12011081 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGGCA AGGCACACAG GCTGAGCGCC GAGGAGCGAG ACCAGCTGCT GCCAAACCTG 
AGGGCTGTGG GGTGGAATGA AGTAGAAGGC CGAGATGCTA TCTTCAAGCA GTTCCATTTT 
AAAGACTTCA ACAGGGCTTT TGGCTTCATG ACAAGAGTAG CCCTGCAGGC TGAAAAGCTG 
GACCACCATC CCGAGTGGTT TAACGTGTAC AACAAGGTCC ATATCACCTT GAGCACCCAT 
GAATGTGCCG GTCTTTCGGA ACGGGATATA AACCTGGCCA GCTTCATCGA ACAAGTCGCC 
GTGTCTATGA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079549.1
CDS	84..398
Translation	MAGKAHRLSAEERDQLLPNLRAVGWNEVEGRDAIFKQFHFKDFNRAFGFMTRVALQAEKLDHHPEWFNVYNKVHITLSTHECAGLSERDINLASFIEQVAVSMT

Target ORF information:

RefSeq Version	NM_025273.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus pterin 4 alpha carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) 1 (Pcbd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025273.4

ORF Insert Sequence:

ATGGCCGGCA AGGCACACAG GCTGAGCGCC GAGGAGCGAG ACCAGCTGCT GCCAAACCTG 
AGGGCTGTGG GGTGGAATGA AGTAGAAGGC CGAGATGCTA TCTTCAAGCA GTTCCATTTT 
AAAGACTTCA ACAGGGCTTT TGGCTTCATG ACAAGAGTAG CCCTGCAGGC TGAAAAGCTG 
GACCACCATC CCGAGTGGTT TAACGTGTAC AACAAGGTCC ATATCACCTT GAGCACCCAT 
GAATGTGCCG GTCTTTCGGA ACGGGATATA AACCTGGCCA GCTTCATCGA ACAAGTCGCC 
GTGTCTATGA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

?-Cell-Specific Mafk Overexpression Impairs Pancreatic Endocrine Cell Development.
PloS one11(2)e0150010(2016)
Abdellatif AM,Oishi H,Itagaki T,Jung Y,Shawki HH,Okita Y,Hasegawa Y,Suzuki H,El-Morsy SE,El-Sayed MA,Shoaib MB,Sugiyama F,Takahashi S

Recessive mutations in PCBD1 cause a new type of early-onset diabetes.
Diabetes63(10)3557-64(2014 Oct)
Simaite D,Kofent J,Gong M,R?schendorf F,Jia S,Arn P,Bentler K,Ellaway C,K?hnen P,Hoffmann GF,Blau N,Spagnoli FM,H?bner N,Raile K

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

An expression profile analysis of ES cell-derived definitive endodermal cells and Pdx1-expressing cells.
BMC developmental biology1113(2011 Mar)
Ogaki S,Harada S,Shiraki N,Kume K,Kume S

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

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Pcbd1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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