Usp8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usp8
Entrez Gene ID	296121
Full Name	ubiquitin specific peptidase 8
General protein information	Preferred Names ubiquitin specific peptidase 8 Names ubiquitin carboxyl-terminal hydrolase 8 ubiquitin specific protease 8
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q36

mRNA and Protein(s)

mRNA	Protein	Name
XM_006234916.3	XP_006234978.1	ubiquitin carboxyl-terminal hydrolase 8 isoform X1
XM_008762155.2	XP_008760377.1	ubiquitin carboxyl-terminal hydrolase 8 isoform X2
NM_001106502.1	NP_001099972.1	ubiquitin carboxyl-terminal hydrolase 8

Pathways from BioSystems

Homologies

Homo sapiens (human)	USP8	NP_005145.3
Drosophila melanogaster (fruit fly)	Ubpy	NP_650948.2
Mus musculus (house mouse)	Usp8	NP_001239509.1
Rattus norvegicus (Norway rat)	Usp8	NP_001099972.1
Gallus gallus (chicken)	USP8	XP_003641901.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002900	XP_312004.5
Caenorhabditis elegans (roundworm)	E01B7.1	NP_507513.2
Pan troglodytes (chimpanzee)	USP8	XP_001168786.1
Canis lupus familiaris (dog)	USP8	XP_535474.1
Bos taurus (cattle)	USP8	NP_001069594.1
Danio rerio (zebrafish)	usp8	XP_693811.5
Xenopus tropicalis (tropical clawed frog)	usp8	XP_004912669.1
	USP8	XP_001114516.1

Gene Ontology
Bibliography

Related articles in PubMed

The Endosome-associated Deubiquitinating Enzyme USP8 Regulates BACE1 Enzyme Ubiquitination and Degradation.
Yeates EF, Tesco G
The Journal of biological chemistry291(30)15753-66(2016 07)

The three 'P's of mitophagy: PARKIN, PINK1, and post-translational modifications.
Durcan TM, Fon EA
Genes & development29(10)989-99(2015 May)

The deubiquitinating enzyme UBPy/USP8 interacts with TrkA and inhibits neuronal differentiation in PC12 cells.
Ceriani M, Amigoni L, D'Aloia A, Berruti G, Martegani E
Experimental cell research333(1)49-59(2015 Apr)

Synaptic strength is bidirectionally controlled by opposing activity-dependent regulation of Nedd4-1 and USP8.
Scudder SL, Goo MS, Cartier AE, Molteni A, Schwarz LA, Wright R, Patrick GN
The Journal of neuroscience : the official journal of the Society for Neuroscience34(50)16637-49(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

USP8 knockdown increases TrkA half-life, suggesting that the deubiquitinating activity of USP8 promotes TrkA degradation.
Title: The deubiquitinating enzyme UBPy/USP8 interacts with TrkA and inhibits neuronal differentiation in PC12 cells.

Homeostatic downscaling of synaptic strength is accompanied by an increase and decrease in Nedd4-1 and USP8 levels, respectively.
Title: Synaptic strength is bidirectionally controlled by opposing activity-dependent regulation of Nedd4-1 and USP8.

The following Usp8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa26320	NM_001106502.1 Latest version!	Rattus norvegicus ubiquitin specific peptidase 8 (Usp8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa26320	XM_006234916.3 Latest version!	Rattus norvegicus ubiquitin specific peptidase 8 (Usp8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa26320	XM_008762155.2 Latest version!	Rattus norvegicus ubiquitin specific peptidase 8 (Usp8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usp8 ( NM_001106502.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099972.1 Rattus norvegicus ubiquitin specific peptidase 8 (Usp8), mRNA.

Price & Availability↑

CloneID	ORa26320
Clone ID Related Accession (Same CDS sequence)	XM_006234916.3 , XM_008762155.2 , NM_001106502.1
Accession Version	NM_001106502.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin carboxyl-terminal hydrolase 8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473949.1. On or before Oct 4, 2007 this sequence version replaced XM_215821.4, XM_001078286.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGCCTGCTG TAGCTTCAGT TCCTAAAGAA CTCTACCTCA GTTCTTCACT CAAAGACCTC 
AATAAGAAGA CAGAAGTTAA ACCTGAGAAA ACCAGCACCA AGAATTATAT ACACAGCGCT 
CAGAAGATTT TCAAGGCAGC AGAAGAATGC AGACTAGATC GTGATGAGGA AAGGGCCTAT 
GTGCTTTATA TGAAATATGT GGCGGTTTAT AATCTTATCA AAAAGAGACC TGATTTCAAG 
CAACAGCAGG ACTATTACCT TTCAATACTT GGACCTGCAA ACATCAAAAA GGCTATTGAA 
GAAGCTGAAA GACTCTCTGA GAGCCTCAAA CTGAGATACG AAGAGGCTGA AGTTCGGAAA 
CAGCTTGAAG AAAAGGACAG ACGGGAGGAA GAACAGCTGC AGCAACAGAA AAGGCAGGAG 
ATGGGAAGAG AGGATAGTGG TGCGGCGGCC AAACGCTCCG TGGAAAATTT ACTGGATTCC 
AAAACCAAAA TCCAAAGGGT CAATGGAGAG AAGAGCGAAG GAGGTGCAGC AGCAGAGAGA 
GGAGCAGTCA CAGCAAAGGA GCTATATACA ATGATGATGG ATAAAAACAC AAGCCTGATT 
ATAATGGATG CTCGGAAAAT ACAGGATTAT CAGCATTCCT GTATCTTGGG CTCTCTCAGT 
GTTCCTGAAG AAGCTATCAG TCCAGGAGTC ACTGCCAATT GGATTGAAGC AAAGCTTTCA 
GATGATTCTA AAGACACGTG GAAAAAAAGG GGGAGTGTGG ACTATGTGGT CCTGCTGGAC 
TGGTTTAGTT CAGCGAAAGA TTTGCTGCTC GGGACCACGC TACGGAGTCT GAAAGATGCT 
CTTTTCAAGT GGGAAAGTAA AGCTGTCCTG CACCATGAGC CCTTGGTGCT GGAGGGCGGC 
TATGAGAACT GGCTCCTGTG CTATCCACAG TTCACAACAA ATGCTAAGGT TACTCCACCC 
CCTCGGAGCA GGCCTGAAGA GGTGTCTGTC TCATTGGATT TTACTTATCC CTCACTGGAA 
GAACCAGTTC CTTCCAAACT TCCTGCCCAG ATGCCACCTC CTCCTTTAGA AGCAAATGAG 
AAGGCACAGT TGGTAACTGA TCAAGATGGT AAACCGAGAC CGTTAGTCCA GTCGGCTCTA 
GCCGGACCCA GTGTGGCTCC CAAAGCTGAA GCTTCACCCA TAATTCAGCC AGTGCCTGCT 
ACAAAGAATG TTCCACAGGT TGATCGTACA AAAAAACCAG CAGTTAAGTT GCCTGAAGAT 
CATAGAATGA AATCTGAAAG TACAGATCAG AGTGGAAGAG TTCTTTCTGA CCGATCCACC 
AAGCCAGTAT TTACCTCTCC AGCCACCATG CTAACAGATG AAGAAAAGGC TCGCATTCAT 
GAAGAAACAG CCCTTCTTAT GGAAAAGAAT AGACAGGAGA AGGAACTGTG GGAAAGGCAG 
CAGAAGGAAC AGAAAGAGAA GCTGAGAAGG GAGGAACAAG AGCGCAAAGC TGGAAAGACC 
CAGGATGCAG AAGAACGGGA CTTCACTGAG AATCAGCACA AAGCAAAGGA TGGACAAGAG 
AAGAGAGACA GCAAACAGAC CAAGGCAGAA GACAGAGAGC CCCCAGCAGA TGGGGCCCAG 
GACGCCACAG GAACGCAAAG ACAAAGTAAG AGTGAACACG ATGCTTCCGA TGCTAAGGTA 
TCTGTGGAAG GTAAAAGGTG TCCCATGTCA GAAGTGCAGA AGAGGCCAGC AGATGTGTCG 
TCTGCATCCT CCATGTCGGG AGAGCTGAGT GCAGGCAAGG CTCAGCGAGA ACCTTTGACA 
AGAGCAAGAA GTGAAGAAAT GGGGAGAATT GTGCCAGGAC TGCCTTTAGG CTGGGCCAAG 
TTTCTTGATC CAATCACTGG GACTTTTCGT TACTACCATT CACCCACAAA CACGGTTCAC 
ATGTATCCGC CTGAGATGGC TCCTTCGTCT GTGCCTCCTT CCACCCCTCC CACTCACAAA 
GTCAAGCCCC AGATCCCTGC TGAGCGGGAC AGGGAGCCAT CCAAACTGAA GCGCTCCTAC 
TCCTCACCAG ACATCACTCA GGCCCTGCAG GAGGAGGAGA AGAGGAGGCC AGCAGTGACC 
CCGACAGTCA ACCGGGAGAA CAAGCCGCCG TGTTACCCTA AAGCTGAGAT CTCGAGGCTT 
TCTGCTTCTC AGATTCGGAA CCTCAATCCC GTTTTTGGAG GATCTGGACC AGCTCTTACT 
GGACTTCGTA ATTTGGGAAA TACTTGTTAC ATGAACTCAA TATTACAGTG CCTGTGCAAT 
GCTCCACATT TGGCTGATTA TTTCAACCGA AACTGCTACC AGGACGATAT CAACAGGTCA 
AATTTGTTGG GGCATAAAGG TGAAGTGGCA GAAGAATTTG GTATAATCAT GAAGGCACTG 
TGGACAGGAC AGTATAGATA CATCAGTCCA AAAGACTTTA AAGTCACCAT TGGTAAGATT 
AATGACCAGT TTGCAGGATC CAGCCAGCAA GATTCACAAG AGCTGCTGCT TTTCCTCATG 
GATGGCCTCC ACGAGGATCT AAATAAGGCT GACAATCGGA AGAGGCATAA GGAAGAGAAC 
AATGACCACC TCGATGACTT TAAAGCGGCA GAACATGCCT GGCAGAAGCA CAAGCAGCTC 
AATGAGTCCA TTATTGTCGC ACTCTTTCAG GGTCAGTTCA AATCCACAGT GCAGTGCCTC 
ACCTGTCACA AGAAGTCTCG GACATTCGAG GCTTTCATGT ATTTGTCTTT GCCACTAGCA 
TCCACAAGTA AATGTACTTT ACAGGACTGC CTTAGATTAT TTTCCAAAGA AGAAAAGCTT 
ACAGATAACA ACAGATTTTA CTGCAGCCAT TGCCGAGCTC GGCGGGATTC TCTAAAGAAA 
ATAGAAATCT GGAAATTGCC TCCTGTGCTG TTAGTACACC TGAAACGATT CTCCTATGAC 
GGCAGGTGGA AGCAGAAACT GCAAACGTCC GTGGATTTCC CATTAGAAAA TCTTGACCTG 
TCACAGTATG TTATTGGCCC AAAAAGCAGT TTGAAGAAAT ATAACTTGTT TTCTGTTTCA 
AACCACTACG GCGGGCTCGA TGGAGGCCAC TACACGGCCT ATTGTAAAAA CGCGGCGAGG 
CAGCGCTGGT TTAAGTTTGA TGACCACGAG GTTTCGGACA TCTCCGTGTC TTCTGTGAGG 
TCGTCAGCAG CTTATATCCT GTTTTACACC TCCCTGGGGC CACGCGTGAC TGAGGCAGCC 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099972.1
CDS	155..3400
Translation	MPAVASVPKELYLSSSLKDLNKKTEVKPEKTSTKNYIHSAQKIFKAAEECRLDRDEERAYVLYMKYVAVYNLIKKRPDFKQQQDYYLSILGPANIKKAIEEAERLSESLKLRYEEAEVRKQLEEKDRREEEQLQQQKRQEMGREDSGAAAKRSVENLLDSKTKIQRVNGEKSEGGAAAERGAVTAKELYTMMMDKNTSLIIMDARKIQDYQHSCILGSLSVPEEAISPGVTANWIEAKLSDDSKDTWKKRGSVDYVVLLDWFSSAKDLLLGTTLRSLKDALFKWESKAVLHHEPLVLEGGYENWLLCYPQFTTNAKVTPPPRSRPEEVSVSLDFTYPSLEEPVPSKLPAQMPPPPLEANEKAQLVTDQDGKPRPLVQSALAGPSVAPKAEASPIIQPVPATKNVPQVDRTKKPAVKLPEDHRMKSESTDQSGRVLSDRSTKPVFTSPATMLTDEEKARIHEETALLMEKNRQEKELWERQQKEQKEKLRREEQERKAGKTQDAEERDFTENQHKAKDGQEKRDSKQTKAEDREPPADGAQDATGTQRQSKSEHDASDAKVSVEGKRCPMSEVQKRPADVSSASSMSGELSAGKAQREPLTRARSEEMGRIVPGLPLGWAKFLDPITGTFRYYHSPTNTVHMYPPEMAPSSVPPSTPPTHKVKPQIPAERDREPSKLKRSYSSPDITQALQEEEKRRPAVTPTVNRENKPPCYPKAEISRLSASQIRNLNPVFGGSGPALTGLRNLGNTCYMNSILQCLCNAPHLADYFNRNCYQDDINRSNLLGHKGEVAEEFGIIMKALWTGQYRYISPKDFKVTIGKINDQFAGSSQQDSQELLLFLMDGLHEDLNKADNRKRHKEENNDHLDDFKAAEHAWQKHKQLNESIIVALFQGQFKSTVQCLTCHKKSRTFEAFMYLSLPLASTSKCTLQDCLRLFSKEEKLTDNNRFYCSHCRARRDSLKKIEIWKLPPVLLVHLKRFSYDGRWKQKLQTSVDFPLENLDLSQYVIGPKSSLKKYNLFSVSNHYGGLDGGHYTAYCKNAARQRWFKFDDHEVSDISVSSVRSSAAYILFYTSLGPRVTEAAT

Target ORF information:

RefSeq Version	NM_001106502.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 8 (Usp8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106502.1

ORF Insert Sequence:

ATGCCTGCTG TAGCTTCAGT TCCTAAAGAA CTCTACCTCA GTTCTTCACT CAAAGACCTC 
AATAAGAAGA CAGAAGTTAA ACCTGAGAAA ACCAGCACCA AGAATTATAT ACACAGCGCT 
CAGAAGATTT TCAAGGCAGC AGAAGAATGC AGACTAGATC GTGATGAGGA AAGGGCCTAT 
GTGCTTTATA TGAAATATGT GGCGGTTTAT AATCTTATCA AAAAGAGACC TGATTTCAAG 
CAACAGCAGG ACTATTACCT TTCAATACTT GGACCTGCAA ACATCAAAAA GGCTATTGAA 
GAAGCTGAAA GACTCTCTGA GAGCCTCAAA CTGAGATACG AAGAGGCTGA AGTTCGGAAA 
CAGCTTGAAG AAAAGGACAG ACGGGAGGAA GAACAGCTGC AGCAACAGAA AAGGCAGGAG 
ATGGGAAGAG AGGATAGTGG TGCGGCGGCC AAACGCTCCG TGGAAAATTT ACTGGATTCC 
AAAACCAAAA TCCAAAGGGT CAATGGAGAG AAGAGCGAAG GAGGTGCAGC AGCAGAGAGA 
GGAGCAGTCA CAGCAAAGGA GCTATATACA ATGATGATGG ATAAAAACAC AAGCCTGATT 
ATAATGGATG CTCGGAAAAT ACAGGATTAT CAGCATTCCT GTATCTTGGG CTCTCTCAGT 
GTTCCTGAAG AAGCTATCAG TCCAGGAGTC ACTGCCAATT GGATTGAAGC AAAGCTTTCA 
GATGATTCTA AAGACACGTG GAAAAAAAGG GGGAGTGTGG ACTATGTGGT CCTGCTGGAC 
TGGTTTAGTT CAGCGAAAGA TTTGCTGCTC GGGACCACGC TACGGAGTCT GAAAGATGCT 
CTTTTCAAGT GGGAAAGTAA AGCTGTCCTG CACCATGAGC CCTTGGTGCT GGAGGGCGGC 
TATGAGAACT GGCTCCTGTG CTATCCACAG TTCACAACAA ATGCTAAGGT TACTCCACCC 
CCTCGGAGCA GGCCTGAAGA GGTGTCTGTC TCATTGGATT TTACTTATCC CTCACTGGAA 
GAACCAGTTC CTTCCAAACT TCCTGCCCAG ATGCCACCTC CTCCTTTAGA AGCAAATGAG 
AAGGCACAGT TGGTAACTGA TCAAGATGGT AAACCGAGAC CGTTAGTCCA GTCGGCTCTA 
GCCGGACCCA GTGTGGCTCC CAAAGCTGAA GCTTCACCCA TAATTCAGCC AGTGCCTGCT 
ACAAAGAATG TTCCACAGGT TGATCGTACA AAAAAACCAG CAGTTAAGTT GCCTGAAGAT 
CATAGAATGA AATCTGAAAG TACAGATCAG AGTGGAAGAG TTCTTTCTGA CCGATCCACC 
AAGCCAGTAT TTACCTCTCC AGCCACCATG CTAACAGATG AAGAAAAGGC TCGCATTCAT 
GAAGAAACAG CCCTTCTTAT GGAAAAGAAT AGACAGGAGA AGGAACTGTG GGAAAGGCAG 
CAGAAGGAAC AGAAAGAGAA GCTGAGAAGG GAGGAACAAG AGCGCAAAGC TGGAAAGACC 
CAGGATGCAG AAGAACGGGA CTTCACTGAG AATCAGCACA AAGCAAAGGA TGGACAAGAG 
AAGAGAGACA GCAAACAGAC CAAGGCAGAA GACAGAGAGC CCCCAGCAGA TGGGGCCCAG 
GACGCCACAG GAACGCAAAG ACAAAGTAAG AGTGAACACG ATGCTTCCGA TGCTAAGGTA 
TCTGTGGAAG GTAAAAGGTG TCCCATGTCA GAAGTGCAGA AGAGGCCAGC AGATGTGTCG 
TCTGCATCCT CCATGTCGGG AGAGCTGAGT GCAGGCAAGG CTCAGCGAGA ACCTTTGACA 
AGAGCAAGAA GTGAAGAAAT GGGGAGAATT GTGCCAGGAC TGCCTTTAGG CTGGGCCAAG 
TTTCTTGATC CAATCACTGG GACTTTTCGT TACTACCATT CACCCACAAA CACGGTTCAC 
ATGTATCCGC CTGAGATGGC TCCTTCGTCT GTGCCTCCTT CCACCCCTCC CACTCACAAA 
GTCAAGCCCC AGATCCCTGC TGAGCGGGAC AGGGAGCCAT CCAAACTGAA GCGCTCCTAC 
TCCTCACCAG ACATCACTCA GGCCCTGCAG GAGGAGGAGA AGAGGAGGCC AGCAGTGACC 
CCGACAGTCA ACCGGGAGAA CAAGCCGCCG TGTTACCCTA AAGCTGAGAT CTCGAGGCTT 
TCTGCTTCTC AGATTCGGAA CCTCAATCCC GTTTTTGGAG GATCTGGACC AGCTCTTACT 
GGACTTCGTA ATTTGGGAAA TACTTGTTAC ATGAACTCAA TATTACAGTG CCTGTGCAAT 
GCTCCACATT TGGCTGATTA TTTCAACCGA AACTGCTACC AGGACGATAT CAACAGGTCA 
AATTTGTTGG GGCATAAAGG TGAAGTGGCA GAAGAATTTG GTATAATCAT GAAGGCACTG 
TGGACAGGAC AGTATAGATA CATCAGTCCA AAAGACTTTA AAGTCACCAT TGGTAAGATT 
AATGACCAGT TTGCAGGATC CAGCCAGCAA GATTCACAAG AGCTGCTGCT TTTCCTCATG 
GATGGCCTCC ACGAGGATCT AAATAAGGCT GACAATCGGA AGAGGCATAA GGAAGAGAAC 
AATGACCACC TCGATGACTT TAAAGCGGCA GAACATGCCT GGCAGAAGCA CAAGCAGCTC 
AATGAGTCCA TTATTGTCGC ACTCTTTCAG GGTCAGTTCA AATCCACAGT GCAGTGCCTC 
ACCTGTCACA AGAAGTCTCG GACATTCGAG GCTTTCATGT ATTTGTCTTT GCCACTAGCA 
TCCACAAGTA AATGTACTTT ACAGGACTGC CTTAGATTAT TTTCCAAAGA AGAAAAGCTT 
ACAGATAACA ACAGATTTTA CTGCAGCCAT TGCCGAGCTC GGCGGGATTC TCTAAAGAAA 
ATAGAAATCT GGAAATTGCC TCCTGTGCTG TTAGTACACC TGAAACGATT CTCCTATGAC 
GGCAGGTGGA AGCAGAAACT GCAAACGTCC GTGGATTTCC CATTAGAAAA TCTTGACCTG 
TCACAGTATG TTATTGGCCC AAAAAGCAGT TTGAAGAAAT ATAACTTGTT TTCTGTTTCA 
AACCACTACG GCGGGCTCGA TGGAGGCCAC TACACGGCCT ATTGTAAAAA CGCGGCGAGG 
CAGCGCTGGT TTAAGTTTGA TGACCACGAG GTTTCGGACA TCTCCGTGTC TTCTGTGAGG 
TCGTCAGCAG CTTATATCCT GTTTTACACC TCCCTGGGGC CACGCGTGAC TGAGGCAGCC 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp8 ( XM_006234916.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234978.1 Rattus norvegicus ubiquitin specific peptidase 8 (Usp8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa26320
Clone ID Related Accession (Same CDS sequence)	XM_006234916.3 , XM_008762155.2 , NM_001106502.1
Accession Version	XM_006234916.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin carboxyl-terminal hydrolase 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234916.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGCTG TAGCTTCAGT TCCTAAAGAA CTCTACCTCA GTTCTTCACT CAAAGACCTC 
AATAAGAAGA CAGAAGTTAA ACCTGAGAAA ACCAGCACCA AGAATTATAT ACACAGCGCT 
CAGAAGATTT TCAAGGCAGC AGAAGAATGC AGACTAGATC GTGATGAGGA AAGGGCCTAT 
GTGCTTTATA TGAAATATGT GGCGGTTTAT AATCTTATCA AAAAGAGACC TGATTTCAAG 
CAACAGCAGG ACTATTACCT TTCAATACTT GGACCTGCAA ACATCAAAAA GGCTATTGAA 
GAAGCTGAAA GACTCTCTGA GAGCCTCAAA CTGAGATACG AAGAGGCTGA AGTTCGGAAA 
CAGCTTGAAG AAAAGGACAG ACGGGAGGAA GAACAGCTGC AGCAACAGAA AAGGCAGGAG 
ATGGGAAGAG AGGATAGTGG TGCGGCGGCC AAACGCTCCG TGGAAAATTT ACTGGATTCC 
AAAACCAAAA TCCAAAGGGT CAATGGAGAG AAGAGCGAAG GAGGTGCAGC AGCAGAGAGA 
GGAGCAGTCA CAGCAAAGGA GCTATATACA ATGATGATGG ATAAAAACAC AAGCCTGATT 
ATAATGGATG CTCGGAAAAT ACAGGATTAT CAGCATTCCT GTATCTTGGG CTCTCTCAGT 
GTTCCTGAAG AAGCTATCAG TCCAGGAGTC ACTGCCAATT GGATTGAAGC AAAGCTTTCA 
GATGATTCTA AAGACACGTG GAAAAAAAGG GGGAGTGTGG ACTATGTGGT CCTGCTGGAC 
TGGTTTAGTT CAGCGAAAGA TTTGCTGCTC GGGACCACGC TACGGAGTCT GAAAGATGCT 
CTTTTCAAGT GGGAAAGTAA AGCTGTCCTG CACCATGAGC CCTTGGTGCT GGAGGGCGGC 
TATGAGAACT GGCTCCTGTG CTATCCACAG TTCACAACAA ATGCTAAGGT TACTCCACCC 
CCTCGGAGCA GGCCTGAAGA GGTGTCTGTC TCATTGGATT TTACTTATCC CTCACTGGAA 
GAACCAGTTC CTTCCAAACT TCCTGCCCAG ATGCCACCTC CTCCTTTAGA AGCAAATGAG 
AAGGCACAGT TGGTAACTGA TCAAGATGGT AAACCGAGAC CGTTAGTCCA GTCGGCTCTA 
GCCGGACCCA GTGTGGCTCC CAAAGCTGAA GCTTCACCCA TAATTCAGCC AGTGCCTGCT 
ACAAAGAATG TTCCACAGGT TGATCGTACA AAAAAACCAG CAGTTAAGTT GCCTGAAGAT 
CATAGAATGA AATCTGAAAG TACAGATCAG AGTGGAAGAG TTCTTTCTGA CCGATCCACC 
AAGCCAGTAT TTACCTCTCC AGCCACCATG CTAACAGATG AAGAAAAGGC TCGCATTCAT 
GAAGAAACAG CCCTTCTTAT GGAAAAGAAT AGACAGGAGA AGGAACTGTG GGAAAGGCAG 
CAGAAGGAAC AGAAAGAGAA GCTGAGAAGG GAGGAACAAG AGCGCAAAGC TGGAAAGACC 
CAGGATGCAG AAGAACGGGA CTTCACTGAG AATCAGCACA AAGCAAAGGA TGGACAAGAG 
AAGAGAGACA GCAAACAGAC CAAGGCAGAA GACAGAGAGC CCCCAGCAGA TGGGGCCCAG 
GACGCCACAG GAACGCAAAG ACAAAGTAAG AGTGAACACG ATGCTTCCGA TGCTAAGGTA 
TCTGTGGAAG GTAAAAGGTG TCCCATGTCA GAAGTGCAGA AGAGGCCAGC AGATGTGTCG 
TCTGCATCCT CCATGTCGGG AGAGCTGAGT GCAGGCAAGG CTCAGCGAGA ACCTTTGACA 
AGAGCAAGAA GTGAAGAAAT GGGGAGAATT GTGCCAGGAC TGCCTTTAGG CTGGGCCAAG 
TTTCTTGATC CAATCACTGG GACTTTTCGT TACTACCATT CACCCACAAA CACGGTTCAC 
ATGTATCCGC CTGAGATGGC TCCTTCGTCT GTGCCTCCTT CCACCCCTCC CACTCACAAA 
GTCAAGCCCC AGATCCCTGC TGAGCGGGAC AGGGAGCCAT CCAAACTGAA GCGCTCCTAC 
TCCTCACCAG ACATCACTCA GGCCCTGCAG GAGGAGGAGA AGAGGAGGCC AGCAGTGACC 
CCGACAGTCA ACCGGGAGAA CAAGCCGCCG TGTTACCCTA AAGCTGAGAT CTCGAGGCTT 
TCTGCTTCTC AGATTCGGAA CCTCAATCCC GTTTTTGGAG GATCTGGACC AGCTCTTACT 
GGACTTCGTA ATTTGGGAAA TACTTGTTAC ATGAACTCAA TATTACAGTG CCTGTGCAAT 
GCTCCACATT TGGCTGATTA TTTCAACCGA AACTGCTACC AGGACGATAT CAACAGGTCA 
AATTTGTTGG GGCATAAAGG TGAAGTGGCA GAAGAATTTG GTATAATCAT GAAGGCACTG 
TGGACAGGAC AGTATAGATA CATCAGTCCA AAAGACTTTA AAGTCACCAT TGGTAAGATT 
AATGACCAGT TTGCAGGATC CAGCCAGCAA GATTCACAAG AGCTGCTGCT TTTCCTCATG 
GATGGCCTCC ACGAGGATCT AAATAAGGCT GACAATCGGA AGAGGCATAA GGAAGAGAAC 
AATGACCACC TCGATGACTT TAAAGCGGCA GAACATGCCT GGCAGAAGCA CAAGCAGCTC 
AATGAGTCCA TTATTGTCGC ACTCTTTCAG GGTCAGTTCA AATCCACAGT GCAGTGCCTC 
ACCTGTCACA AGAAGTCTCG GACATTCGAG GCTTTCATGT ATTTGTCTTT GCCACTAGCA 
TCCACAAGTA AATGTACTTT ACAGGACTGC CTTAGATTAT TTTCCAAAGA AGAAAAGCTT 
ACAGATAACA ACAGATTTTA CTGCAGCCAT TGCCGAGCTC GGCGGGATTC TCTAAAGAAA 
ATAGAAATCT GGAAATTGCC TCCTGTGCTG TTAGTACACC TGAAACGATT CTCCTATGAC 
GGCAGGTGGA AGCAGAAACT GCAAACGTCC GTGGATTTCC CATTAGAAAA TCTTGACCTG 
TCACAGTATG TTATTGGCCC AAAAAGCAGT TTGAAGAAAT ATAACTTGTT TTCTGTTTCA 
AACCACTACG GCGGGCTCGA TGGAGGCCAC TACACGGCCT ATTGTAAAAA CGCGGCGAGG 
CAGCGCTGGT TTAAGTTTGA TGACCACGAG GTTTCGGACA TCTCCGTGTC TTCTGTGAGG 
TCGTCAGCAG CTTATATCCT GTTTTACACC TCCCTGGGGC CACGCGTGAC TGAGGCAGCC 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234978.1
CDS	201..3446
Translation	MPAVASVPKELYLSSSLKDLNKKTEVKPEKTSTKNYIHSAQKIFKAAEECRLDRDEERAYVLYMKYVAVYNLIKKRPDFKQQQDYYLSILGPANIKKAIEEAERLSESLKLRYEEAEVRKQLEEKDRREEEQLQQQKRQEMGREDSGAAAKRSVENLLDSKTKIQRVNGEKSEGGAAAERGAVTAKELYTMMMDKNTSLIIMDARKIQDYQHSCILGSLSVPEEAISPGVTANWIEAKLSDDSKDTWKKRGSVDYVVLLDWFSSAKDLLLGTTLRSLKDALFKWESKAVLHHEPLVLEGGYENWLLCYPQFTTNAKVTPPPRSRPEEVSVSLDFTYPSLEEPVPSKLPAQMPPPPLEANEKAQLVTDQDGKPRPLVQSALAGPSVAPKAEASPIIQPVPATKNVPQVDRTKKPAVKLPEDHRMKSESTDQSGRVLSDRSTKPVFTSPATMLTDEEKARIHEETALLMEKNRQEKELWERQQKEQKEKLRREEQERKAGKTQDAEERDFTENQHKAKDGQEKRDSKQTKAEDREPPADGAQDATGTQRQSKSEHDASDAKVSVEGKRCPMSEVQKRPADVSSASSMSGELSAGKAQREPLTRARSEEMGRIVPGLPLGWAKFLDPITGTFRYYHSPTNTVHMYPPEMAPSSVPPSTPPTHKVKPQIPAERDREPSKLKRSYSSPDITQALQEEEKRRPAVTPTVNRENKPPCYPKAEISRLSASQIRNLNPVFGGSGPALTGLRNLGNTCYMNSILQCLCNAPHLADYFNRNCYQDDINRSNLLGHKGEVAEEFGIIMKALWTGQYRYISPKDFKVTIGKINDQFAGSSQQDSQELLLFLMDGLHEDLNKADNRKRHKEENNDHLDDFKAAEHAWQKHKQLNESIIVALFQGQFKSTVQCLTCHKKSRTFEAFMYLSLPLASTSKCTLQDCLRLFSKEEKLTDNNRFYCSHCRARRDSLKKIEIWKLPPVLLVHLKRFSYDGRWKQKLQTSVDFPLENLDLSQYVIGPKSSLKKYNLFSVSNHYGGLDGGHYTAYCKNAARQRWFKFDDHEVSDISVSSVRSSAAYILFYTSLGPRVTEAAT

Target ORF information:

RefSeq Version	XM_006234916.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 8 (Usp8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234916.3

ORF Insert Sequence:

ATGCCTGCTG TAGCTTCAGT TCCTAAAGAA CTCTACCTCA GTTCTTCACT CAAAGACCTC 
AATAAGAAGA CAGAAGTTAA ACCTGAGAAA ACCAGCACCA AGAATTATAT ACACAGCGCT 
CAGAAGATTT TCAAGGCAGC AGAAGAATGC AGACTAGATC GTGATGAGGA AAGGGCCTAT 
GTGCTTTATA TGAAATATGT GGCGGTTTAT AATCTTATCA AAAAGAGACC TGATTTCAAG 
CAACAGCAGG ACTATTACCT TTCAATACTT GGACCTGCAA ACATCAAAAA GGCTATTGAA 
GAAGCTGAAA GACTCTCTGA GAGCCTCAAA CTGAGATACG AAGAGGCTGA AGTTCGGAAA 
CAGCTTGAAG AAAAGGACAG ACGGGAGGAA GAACAGCTGC AGCAACAGAA AAGGCAGGAG 
ATGGGAAGAG AGGATAGTGG TGCGGCGGCC AAACGCTCCG TGGAAAATTT ACTGGATTCC 
AAAACCAAAA TCCAAAGGGT CAATGGAGAG AAGAGCGAAG GAGGTGCAGC AGCAGAGAGA 
GGAGCAGTCA CAGCAAAGGA GCTATATACA ATGATGATGG ATAAAAACAC AAGCCTGATT 
ATAATGGATG CTCGGAAAAT ACAGGATTAT CAGCATTCCT GTATCTTGGG CTCTCTCAGT 
GTTCCTGAAG AAGCTATCAG TCCAGGAGTC ACTGCCAATT GGATTGAAGC AAAGCTTTCA 
GATGATTCTA AAGACACGTG GAAAAAAAGG GGGAGTGTGG ACTATGTGGT CCTGCTGGAC 
TGGTTTAGTT CAGCGAAAGA TTTGCTGCTC GGGACCACGC TACGGAGTCT GAAAGATGCT 
CTTTTCAAGT GGGAAAGTAA AGCTGTCCTG CACCATGAGC CCTTGGTGCT GGAGGGCGGC 
TATGAGAACT GGCTCCTGTG CTATCCACAG TTCACAACAA ATGCTAAGGT TACTCCACCC 
CCTCGGAGCA GGCCTGAAGA GGTGTCTGTC TCATTGGATT TTACTTATCC CTCACTGGAA 
GAACCAGTTC CTTCCAAACT TCCTGCCCAG ATGCCACCTC CTCCTTTAGA AGCAAATGAG 
AAGGCACAGT TGGTAACTGA TCAAGATGGT AAACCGAGAC CGTTAGTCCA GTCGGCTCTA 
GCCGGACCCA GTGTGGCTCC CAAAGCTGAA GCTTCACCCA TAATTCAGCC AGTGCCTGCT 
ACAAAGAATG TTCCACAGGT TGATCGTACA AAAAAACCAG CAGTTAAGTT GCCTGAAGAT 
CATAGAATGA AATCTGAAAG TACAGATCAG AGTGGAAGAG TTCTTTCTGA CCGATCCACC 
AAGCCAGTAT TTACCTCTCC AGCCACCATG CTAACAGATG AAGAAAAGGC TCGCATTCAT 
GAAGAAACAG CCCTTCTTAT GGAAAAGAAT AGACAGGAGA AGGAACTGTG GGAAAGGCAG 
CAGAAGGAAC AGAAAGAGAA GCTGAGAAGG GAGGAACAAG AGCGCAAAGC TGGAAAGACC 
CAGGATGCAG AAGAACGGGA CTTCACTGAG AATCAGCACA AAGCAAAGGA TGGACAAGAG 
AAGAGAGACA GCAAACAGAC CAAGGCAGAA GACAGAGAGC CCCCAGCAGA TGGGGCCCAG 
GACGCCACAG GAACGCAAAG ACAAAGTAAG AGTGAACACG ATGCTTCCGA TGCTAAGGTA 
TCTGTGGAAG GTAAAAGGTG TCCCATGTCA GAAGTGCAGA AGAGGCCAGC AGATGTGTCG 
TCTGCATCCT CCATGTCGGG AGAGCTGAGT GCAGGCAAGG CTCAGCGAGA ACCTTTGACA 
AGAGCAAGAA GTGAAGAAAT GGGGAGAATT GTGCCAGGAC TGCCTTTAGG CTGGGCCAAG 
TTTCTTGATC CAATCACTGG GACTTTTCGT TACTACCATT CACCCACAAA CACGGTTCAC 
ATGTATCCGC CTGAGATGGC TCCTTCGTCT GTGCCTCCTT CCACCCCTCC CACTCACAAA 
GTCAAGCCCC AGATCCCTGC TGAGCGGGAC AGGGAGCCAT CCAAACTGAA GCGCTCCTAC 
TCCTCACCAG ACATCACTCA GGCCCTGCAG GAGGAGGAGA AGAGGAGGCC AGCAGTGACC 
CCGACAGTCA ACCGGGAGAA CAAGCCGCCG TGTTACCCTA AAGCTGAGAT CTCGAGGCTT 
TCTGCTTCTC AGATTCGGAA CCTCAATCCC GTTTTTGGAG GATCTGGACC AGCTCTTACT 
GGACTTCGTA ATTTGGGAAA TACTTGTTAC ATGAACTCAA TATTACAGTG CCTGTGCAAT 
GCTCCACATT TGGCTGATTA TTTCAACCGA AACTGCTACC AGGACGATAT CAACAGGTCA 
AATTTGTTGG GGCATAAAGG TGAAGTGGCA GAAGAATTTG GTATAATCAT GAAGGCACTG 
TGGACAGGAC AGTATAGATA CATCAGTCCA AAAGACTTTA AAGTCACCAT TGGTAAGATT 
AATGACCAGT TTGCAGGATC CAGCCAGCAA GATTCACAAG AGCTGCTGCT TTTCCTCATG 
GATGGCCTCC ACGAGGATCT AAATAAGGCT GACAATCGGA AGAGGCATAA GGAAGAGAAC 
AATGACCACC TCGATGACTT TAAAGCGGCA GAACATGCCT GGCAGAAGCA CAAGCAGCTC 
AATGAGTCCA TTATTGTCGC ACTCTTTCAG GGTCAGTTCA AATCCACAGT GCAGTGCCTC 
ACCTGTCACA AGAAGTCTCG GACATTCGAG GCTTTCATGT ATTTGTCTTT GCCACTAGCA 
TCCACAAGTA AATGTACTTT ACAGGACTGC CTTAGATTAT TTTCCAAAGA AGAAAAGCTT 
ACAGATAACA ACAGATTTTA CTGCAGCCAT TGCCGAGCTC GGCGGGATTC TCTAAAGAAA 
ATAGAAATCT GGAAATTGCC TCCTGTGCTG TTAGTACACC TGAAACGATT CTCCTATGAC 
GGCAGGTGGA AGCAGAAACT GCAAACGTCC GTGGATTTCC CATTAGAAAA TCTTGACCTG 
TCACAGTATG TTATTGGCCC AAAAAGCAGT TTGAAGAAAT ATAACTTGTT TTCTGTTTCA 
AACCACTACG GCGGGCTCGA TGGAGGCCAC TACACGGCCT ATTGTAAAAA CGCGGCGAGG 
CAGCGCTGGT TTAAGTTTGA TGACCACGAG GTTTCGGACA TCTCCGTGTC TTCTGTGAGG 
TCGTCAGCAG CTTATATCCT GTTTTACACC TCCCTGGGGC CACGCGTGAC TGAGGCAGCC 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp8 ( XM_008762155.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760377.1 Rattus norvegicus ubiquitin specific peptidase 8 (Usp8), transcript variant X2, mRNA.

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CloneID	ORa26320
Clone ID Related Accession (Same CDS sequence)	XM_006234916.3 , XM_008762155.2 , NM_001106502.1
Accession Version	XM_008762155.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin carboxyl-terminal hydrolase 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762155.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGCTG TAGCTTCAGT TCCTAAAGAA CTCTACCTCA GTTCTTCACT CAAAGACCTC 
AATAAGAAGA CAGAAGTTAA ACCTGAGAAA ACCAGCACCA AGAATTATAT ACACAGCGCT 
CAGAAGATTT TCAAGGCAGC AGAAGAATGC AGACTAGATC GTGATGAGGA AAGGGCCTAT 
GTGCTTTATA TGAAATATGT GGCGGTTTAT AATCTTATCA AAAAGAGACC TGATTTCAAG 
CAACAGCAGG ACTATTACCT TTCAATACTT GGACCTGCAA ACATCAAAAA GGCTATTGAA 
GAAGCTGAAA GACTCTCTGA GAGCCTCAAA CTGAGATACG AAGAGGCTGA AGTTCGGAAA 
CAGCTTGAAG AAAAGGACAG ACGGGAGGAA GAACAGCTGC AGCAACAGAA AAGGCAGGAG 
ATGGGAAGAG AGGATAGTGG TGCGGCGGCC AAACGCTCCG TGGAAAATTT ACTGGATTCC 
AAAACCAAAA TCCAAAGGGT CAATGGAGAG AAGAGCGAAG GAGGTGCAGC AGCAGAGAGA 
GGAGCAGTCA CAGCAAAGGA GCTATATACA ATGATGATGG ATAAAAACAC AAGCCTGATT 
ATAATGGATG CTCGGAAAAT ACAGGATTAT CAGCATTCCT GTATCTTGGG CTCTCTCAGT 
GTTCCTGAAG AAGCTATCAG TCCAGGAGTC ACTGCCAATT GGATTGAAGC AAAGCTTTCA 
GATGATTCTA AAGACACGTG GAAAAAAAGG GGGAGTGTGG ACTATGTGGT CCTGCTGGAC 
TGGTTTAGTT CAGCGAAAGA TTTGCTGCTC GGGACCACGC TACGGAGTCT GAAAGATGCT 
CTTTTCAAGT GGGAAAGTAA AGCTGTCCTG CACCATGAGC CCTTGGTGCT GGAGGGCGGC 
TATGAGAACT GGCTCCTGTG CTATCCACAG TTCACAACAA ATGCTAAGGT TACTCCACCC 
CCTCGGAGCA GGCCTGAAGA GGTGTCTGTC TCATTGGATT TTACTTATCC CTCACTGGAA 
GAACCAGTTC CTTCCAAACT TCCTGCCCAG ATGCCACCTC CTCCTTTAGA AGCAAATGAG 
AAGGCACAGT TGGTAACTGA TCAAGATGGT AAACCGAGAC CGTTAGTCCA GTCGGCTCTA 
GCCGGACCCA GTGTGGCTCC CAAAGCTGAA GCTTCACCCA TAATTCAGCC AGTGCCTGCT 
ACAAAGAATG TTCCACAGGT TGATCGTACA AAAAAACCAG CAGTTAAGTT GCCTGAAGAT 
CATAGAATGA AATCTGAAAG TACAGATCAG AGTGGAAGAG TTCTTTCTGA CCGATCCACC 
AAGCCAGTAT TTACCTCTCC AGCCACCATG CTAACAGATG AAGAAAAGGC TCGCATTCAT 
GAAGAAACAG CCCTTCTTAT GGAAAAGAAT AGACAGGAGA AGGAACTGTG GGAAAGGCAG 
CAGAAGGAAC AGAAAGAGAA GCTGAGAAGG GAGGAACAAG AGCGCAAAGC TGGAAAGACC 
CAGGATGCAG AAGAACGGGA CTTCACTGAG AATCAGCACA AAGCAAAGGA TGGACAAGAG 
AAGAGAGACA GCAAACAGAC CAAGGCAGAA GACAGAGAGC CCCCAGCAGA TGGGGCCCAG 
GACGCCACAG GAACGCAAAG ACAAAGTAAG AGTGAACACG ATGCTTCCGA TGCTAAGGTA 
TCTGTGGAAG GTAAAAGGTG TCCCATGTCA GAAGTGCAGA AGAGGCCAGC AGATGTGTCG 
TCTGCATCCT CCATGTCGGG AGAGCTGAGT GCAGGCAAGG CTCAGCGAGA ACCTTTGACA 
AGAGCAAGAA GTGAAGAAAT GGGGAGAATT GTGCCAGGAC TGCCTTTAGG CTGGGCCAAG 
TTTCTTGATC CAATCACTGG GACTTTTCGT TACTACCATT CACCCACAAA CACGGTTCAC 
ATGTATCCGC CTGAGATGGC TCCTTCGTCT GTGCCTCCTT CCACCCCTCC CACTCACAAA 
GTCAAGCCCC AGATCCCTGC TGAGCGGGAC AGGGAGCCAT CCAAACTGAA GCGCTCCTAC 
TCCTCACCAG ACATCACTCA GGCCCTGCAG GAGGAGGAGA AGAGGAGGCC AGCAGTGACC 
CCGACAGTCA ACCGGGAGAA CAAGCCGCCG TGTTACCCTA AAGCTGAGAT CTCGAGGCTT 
TCTGCTTCTC AGATTCGGAA CCTCAATCCC GTTTTTGGAG GATCTGGACC AGCTCTTACT 
GGACTTCGTA ATTTGGGAAA TACTTGTTAC ATGAACTCAA TATTACAGTG CCTGTGCAAT 
GCTCCACATT TGGCTGATTA TTTCAACCGA AACTGCTACC AGGACGATAT CAACAGGTCA 
AATTTGTTGG GGCATAAAGG TGAAGTGGCA GAAGAATTTG GTATAATCAT GAAGGCACTG 
TGGACAGGAC AGTATAGATA CATCAGTCCA AAAGACTTTA AAGTCACCAT TGGTAAGATT 
AATGACCAGT TTGCAGGATC CAGCCAGCAA GATTCACAAG AGCTGCTGCT TTTCCTCATG 
GATGGCCTCC ACGAGGATCT AAATAAGGCT GACAATCGGA AGAGGCATAA GGAAGAGAAC 
AATGACCACC TCGATGACTT TAAAGCGGCA GAACATGCCT GGCAGAAGCA CAAGCAGCTC 
AATGAGTCCA TTATTGTCGC ACTCTTTCAG GGTCAGTTCA AATCCACAGT GCAGTGCCTC 
ACCTGTCACA AGAAGTCTCG GACATTCGAG GCTTTCATGT ATTTGTCTTT GCCACTAGCA 
TCCACAAGTA AATGTACTTT ACAGGACTGC CTTAGATTAT TTTCCAAAGA AGAAAAGCTT 
ACAGATAACA ACAGATTTTA CTGCAGCCAT TGCCGAGCTC GGCGGGATTC TCTAAAGAAA 
ATAGAAATCT GGAAATTGCC TCCTGTGCTG TTAGTACACC TGAAACGATT CTCCTATGAC 
GGCAGGTGGA AGCAGAAACT GCAAACGTCC GTGGATTTCC CATTAGAAAA TCTTGACCTG 
TCACAGTATG TTATTGGCCC AAAAAGCAGT TTGAAGAAAT ATAACTTGTT TTCTGTTTCA 
AACCACTACG GCGGGCTCGA TGGAGGCCAC TACACGGCCT ATTGTAAAAA CGCGGCGAGG 
CAGCGCTGGT TTAAGTTTGA TGACCACGAG GTTTCGGACA TCTCCGTGTC TTCTGTGAGG 
TCGTCAGCAG CTTATATCCT GTTTTACACC TCCCTGGGGC CACGCGTGAC TGAGGCAGCC 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760377.1
CDS	201..3446
Translation	MPAVASVPKELYLSSSLKDLNKKTEVKPEKTSTKNYIHSAQKIFKAAEECRLDRDEERAYVLYMKYVAVYNLIKKRPDFKQQQDYYLSILGPANIKKAIEEAERLSESLKLRYEEAEVRKQLEEKDRREEEQLQQQKRQEMGREDSGAAAKRSVENLLDSKTKIQRVNGEKSEGGAAAERGAVTAKELYTMMMDKNTSLIIMDARKIQDYQHSCILGSLSVPEEAISPGVTANWIEAKLSDDSKDTWKKRGSVDYVVLLDWFSSAKDLLLGTTLRSLKDALFKWESKAVLHHEPLVLEGGYENWLLCYPQFTTNAKVTPPPRSRPEEVSVSLDFTYPSLEEPVPSKLPAQMPPPPLEANEKAQLVTDQDGKPRPLVQSALAGPSVAPKAEASPIIQPVPATKNVPQVDRTKKPAVKLPEDHRMKSESTDQSGRVLSDRSTKPVFTSPATMLTDEEKARIHEETALLMEKNRQEKELWERQQKEQKEKLRREEQERKAGKTQDAEERDFTENQHKAKDGQEKRDSKQTKAEDREPPADGAQDATGTQRQSKSEHDASDAKVSVEGKRCPMSEVQKRPADVSSASSMSGELSAGKAQREPLTRARSEEMGRIVPGLPLGWAKFLDPITGTFRYYHSPTNTVHMYPPEMAPSSVPPSTPPTHKVKPQIPAERDREPSKLKRSYSSPDITQALQEEEKRRPAVTPTVNRENKPPCYPKAEISRLSASQIRNLNPVFGGSGPALTGLRNLGNTCYMNSILQCLCNAPHLADYFNRNCYQDDINRSNLLGHKGEVAEEFGIIMKALWTGQYRYISPKDFKVTIGKINDQFAGSSQQDSQELLLFLMDGLHEDLNKADNRKRHKEENNDHLDDFKAAEHAWQKHKQLNESIIVALFQGQFKSTVQCLTCHKKSRTFEAFMYLSLPLASTSKCTLQDCLRLFSKEEKLTDNNRFYCSHCRARRDSLKKIEIWKLPPVLLVHLKRFSYDGRWKQKLQTSVDFPLENLDLSQYVIGPKSSLKKYNLFSVSNHYGGLDGGHYTAYCKNAARQRWFKFDDHEVSDISVSSVRSSAAYILFYTSLGPRVTEAAT

Target ORF information:

RefSeq Version	XM_008762155.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 8 (Usp8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762155.2

ORF Insert Sequence:

ATGCCTGCTG TAGCTTCAGT TCCTAAAGAA CTCTACCTCA GTTCTTCACT CAAAGACCTC 
AATAAGAAGA CAGAAGTTAA ACCTGAGAAA ACCAGCACCA AGAATTATAT ACACAGCGCT 
CAGAAGATTT TCAAGGCAGC AGAAGAATGC AGACTAGATC GTGATGAGGA AAGGGCCTAT 
GTGCTTTATA TGAAATATGT GGCGGTTTAT AATCTTATCA AAAAGAGACC TGATTTCAAG 
CAACAGCAGG ACTATTACCT TTCAATACTT GGACCTGCAA ACATCAAAAA GGCTATTGAA 
GAAGCTGAAA GACTCTCTGA GAGCCTCAAA CTGAGATACG AAGAGGCTGA AGTTCGGAAA 
CAGCTTGAAG AAAAGGACAG ACGGGAGGAA GAACAGCTGC AGCAACAGAA AAGGCAGGAG 
ATGGGAAGAG AGGATAGTGG TGCGGCGGCC AAACGCTCCG TGGAAAATTT ACTGGATTCC 
AAAACCAAAA TCCAAAGGGT CAATGGAGAG AAGAGCGAAG GAGGTGCAGC AGCAGAGAGA 
GGAGCAGTCA CAGCAAAGGA GCTATATACA ATGATGATGG ATAAAAACAC AAGCCTGATT 
ATAATGGATG CTCGGAAAAT ACAGGATTAT CAGCATTCCT GTATCTTGGG CTCTCTCAGT 
GTTCCTGAAG AAGCTATCAG TCCAGGAGTC ACTGCCAATT GGATTGAAGC AAAGCTTTCA 
GATGATTCTA AAGACACGTG GAAAAAAAGG GGGAGTGTGG ACTATGTGGT CCTGCTGGAC 
TGGTTTAGTT CAGCGAAAGA TTTGCTGCTC GGGACCACGC TACGGAGTCT GAAAGATGCT 
CTTTTCAAGT GGGAAAGTAA AGCTGTCCTG CACCATGAGC CCTTGGTGCT GGAGGGCGGC 
TATGAGAACT GGCTCCTGTG CTATCCACAG TTCACAACAA ATGCTAAGGT TACTCCACCC 
CCTCGGAGCA GGCCTGAAGA GGTGTCTGTC TCATTGGATT TTACTTATCC CTCACTGGAA 
GAACCAGTTC CTTCCAAACT TCCTGCCCAG ATGCCACCTC CTCCTTTAGA AGCAAATGAG 
AAGGCACAGT TGGTAACTGA TCAAGATGGT AAACCGAGAC CGTTAGTCCA GTCGGCTCTA 
GCCGGACCCA GTGTGGCTCC CAAAGCTGAA GCTTCACCCA TAATTCAGCC AGTGCCTGCT 
ACAAAGAATG TTCCACAGGT TGATCGTACA AAAAAACCAG CAGTTAAGTT GCCTGAAGAT 
CATAGAATGA AATCTGAAAG TACAGATCAG AGTGGAAGAG TTCTTTCTGA CCGATCCACC 
AAGCCAGTAT TTACCTCTCC AGCCACCATG CTAACAGATG AAGAAAAGGC TCGCATTCAT 
GAAGAAACAG CCCTTCTTAT GGAAAAGAAT AGACAGGAGA AGGAACTGTG GGAAAGGCAG 
CAGAAGGAAC AGAAAGAGAA GCTGAGAAGG GAGGAACAAG AGCGCAAAGC TGGAAAGACC 
CAGGATGCAG AAGAACGGGA CTTCACTGAG AATCAGCACA AAGCAAAGGA TGGACAAGAG 
AAGAGAGACA GCAAACAGAC CAAGGCAGAA GACAGAGAGC CCCCAGCAGA TGGGGCCCAG 
GACGCCACAG GAACGCAAAG ACAAAGTAAG AGTGAACACG ATGCTTCCGA TGCTAAGGTA 
TCTGTGGAAG GTAAAAGGTG TCCCATGTCA GAAGTGCAGA AGAGGCCAGC AGATGTGTCG 
TCTGCATCCT CCATGTCGGG AGAGCTGAGT GCAGGCAAGG CTCAGCGAGA ACCTTTGACA 
AGAGCAAGAA GTGAAGAAAT GGGGAGAATT GTGCCAGGAC TGCCTTTAGG CTGGGCCAAG 
TTTCTTGATC CAATCACTGG GACTTTTCGT TACTACCATT CACCCACAAA CACGGTTCAC 
ATGTATCCGC CTGAGATGGC TCCTTCGTCT GTGCCTCCTT CCACCCCTCC CACTCACAAA 
GTCAAGCCCC AGATCCCTGC TGAGCGGGAC AGGGAGCCAT CCAAACTGAA GCGCTCCTAC 
TCCTCACCAG ACATCACTCA GGCCCTGCAG GAGGAGGAGA AGAGGAGGCC AGCAGTGACC 
CCGACAGTCA ACCGGGAGAA CAAGCCGCCG TGTTACCCTA AAGCTGAGAT CTCGAGGCTT 
TCTGCTTCTC AGATTCGGAA CCTCAATCCC GTTTTTGGAG GATCTGGACC AGCTCTTACT 
GGACTTCGTA ATTTGGGAAA TACTTGTTAC ATGAACTCAA TATTACAGTG CCTGTGCAAT 
GCTCCACATT TGGCTGATTA TTTCAACCGA AACTGCTACC AGGACGATAT CAACAGGTCA 
AATTTGTTGG GGCATAAAGG TGAAGTGGCA GAAGAATTTG GTATAATCAT GAAGGCACTG 
TGGACAGGAC AGTATAGATA CATCAGTCCA AAAGACTTTA AAGTCACCAT TGGTAAGATT 
AATGACCAGT TTGCAGGATC CAGCCAGCAA GATTCACAAG AGCTGCTGCT TTTCCTCATG 
GATGGCCTCC ACGAGGATCT AAATAAGGCT GACAATCGGA AGAGGCATAA GGAAGAGAAC 
AATGACCACC TCGATGACTT TAAAGCGGCA GAACATGCCT GGCAGAAGCA CAAGCAGCTC 
AATGAGTCCA TTATTGTCGC ACTCTTTCAG GGTCAGTTCA AATCCACAGT GCAGTGCCTC 
ACCTGTCACA AGAAGTCTCG GACATTCGAG GCTTTCATGT ATTTGTCTTT GCCACTAGCA 
TCCACAAGTA AATGTACTTT ACAGGACTGC CTTAGATTAT TTTCCAAAGA AGAAAAGCTT 
ACAGATAACA ACAGATTTTA CTGCAGCCAT TGCCGAGCTC GGCGGGATTC TCTAAAGAAA 
ATAGAAATCT GGAAATTGCC TCCTGTGCTG TTAGTACACC TGAAACGATT CTCCTATGAC 
GGCAGGTGGA AGCAGAAACT GCAAACGTCC GTGGATTTCC CATTAGAAAA TCTTGACCTG 
TCACAGTATG TTATTGGCCC AAAAAGCAGT TTGAAGAAAT ATAACTTGTT TTCTGTTTCA 
AACCACTACG GCGGGCTCGA TGGAGGCCAC TACACGGCCT ATTGTAAAAA CGCGGCGAGG 
CAGCGCTGGT TTAAGTTTGA TGACCACGAG GTTTCGGACA TCTCCGTGTC TTCTGTGAGG 
TCGTCAGCAG CTTATATCCT GTTTTACACC TCCCTGGGGC CACGCGTGAC TGAGGCAGCC 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Endosome-associated Deubiquitinating Enzyme USP8 Regulates BACE1 Enzyme Ubiquitination and Degradation.
The Journal of biological chemistry291(30)15753-66(2016 07)
Yeates EF,Tesco G

The three 'P's of mitophagy: PARKIN, PINK1, and post-translational modifications.
Genes & development29(10)989-99(2015 May)
Durcan TM,Fon EA

The deubiquitinating enzyme UBPy/USP8 interacts with TrkA and inhibits neuronal differentiation in PC12 cells.
Experimental cell research333(1)49-59(2015 Apr)
Ceriani M,Amigoni L,D'Aloia A,Berruti G,Martegani E

Synaptic strength is bidirectionally controlled by opposing activity-dependent regulation of Nedd4-1 and USP8.
The Journal of neuroscience : the official journal of the Society for Neuroscience34(50)16637-49(2014 Dec)
Scudder SL,Goo MS,Cartier AE,Molteni A,Schwarz LA,Wright R,Patrick GN

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Usp8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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