usp8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following usp8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa43369 XM_018092440.1
Latest version!
Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OXa55132 XM_018092438.2
Latest version!
Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa55132 XM_018092439.2
Latest version!
Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa55132 XM_002939624.5
Latest version!
Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa55132 XM_012959499.3
Latest version!
Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa55132 XM_004912609.4
Latest version!
Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa55133 XM_031899207.1
Latest version!
Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa07224 XM_018092438.1
Latest version!
Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa07224 XM_018092439.1
Latest version!
Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa07224 XM_002939624.4
Latest version!
Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa07224 XM_004912609.3
Latest version!
Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa07224 XM_012959499.2
Latest version!
Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa07224 XM_018092437.1
Latest version!
Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa07225 XM_012959500.2
Latest version!
Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa43369
    Clone ID Related Accession (Same CDS sequence) XM_018092440.1
    Accession Version XM_018092440.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2718bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin carboxyl-terminal hydrolase 8 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGAATG GGGAGAAAAA TGAAGTTCTT TCAAAGCGGC CAGAACCTAC TAATGTGCCT 
    CATGGAGCTA TCACAGTTGA AAAGCTTTTT GAGATGACAA ATGATTATAA TATCAGTATA
    ATTATAATGG ATGTTCGGAG TACAAAAGAC TTTCAGGAGT CTCATATTCG TACTTCAATA
    AGCGTGCCAG AGGAGGCCAT AATCCCAGGA TTTACCTCAA GCAAGATTGA AACTAATCTA
    CCTGATACAT CTAAAGAAAT GTGGAATAAA AGAGGTCAAG TTGATTTTAT TATATTGCTT
    GATTGGTTCA GCTCTGTAAA TAACTTAAAA GTTGGTACAA CACTTCAGAG CTTAAAAGAT
    GCTCTGTATA AGTGGGAAAC AAAAACAATC CTACGGAGTG AACCTTTAGT TTTGGAAGGT
    GGTTATGAAA ATTGGCTTCT TTGTTATCCC ATGTTCACAA CAAATGGGAA AGTGACTGTG
    CCCAGGAAAA GAACACAAGA ATCTGTGTCT TTGTCATTGG ACTTCAGTTA TCCATCTCTA
    GAAGAACCAG CACTTCCACC ACCACCACCA CCCCCTGTAT GCATCAAAGA AAGCAGCTAT
    GAGGATACTA TAAATGCAGT GAAAACTCAA AAAACAGAGG AACCTGTCCA TGTAGTACAA
    AAGGATGACC CTGTACCAGA TGCTCAGACT CCAGATTCTG TTAAAGTCTG TCAGCCACAG
    AAAACTGTGA AAGTTATTCC ACAAATTGAT CGGACAAAGA AACCTTCAGT AAAAGTTACT
    GAAAGTTCCT CAAAGTTTAA ACATGAAGCC AAAGAAAACC AGCCAATACA GAATGGAAGG
    CCTATACCAG ACCGTTCCAC AAAACCATCA CTAGACACTA AATCGGTCAA ATCGGAGATC
    CATGTTCAGT TTGATAAAAA CATAAAGGAA AAGGGTGAAG AAATTAAAGG GAAAGTAGAT
    ATGCATGCAG AAAACAATAT TGATAACACA TGTGCACATG AAGAAAAAGA AAACAAAAAG
    AGGAATGAAG AGAAAGAAAC TGAGATGGAT GAGTTCCACA GAAAGGAGGA GTTAGAAAAA
    AAACAGCCAG TAGTTGCTGG AAAAGAACCT GCTGAAACAA AAAGGCTGGA AAGTAATAAG
    CCTCCCCCTG ATGATAGAAA GAAGAATGGC CATTCTTCTG TTACTCCTCA AGAGCCTTCT
    GCACATAAAA AAGAACATAA TTCTGATGTG ACTGTGAAAG ACAGCTGTGA AGACGACAGA
    CCCAAACGGG AACCTTTAGT TCGAGCAAGA AGTGAGGAGA TGGGTAGAAC TGTGCCAGGA
    ATGCCAGAAG GTTGGGTGAA GTTCCTTGAA CCTGTCACTG GAACCTACCG ATATTATCAT
    TCACCAACAC ACACTGTTCA TATGTATCCT CCTGAAATGG CTCCTTCATC CACCCCTTCA
    ACCACTCCAG CTCAGAAAGC CAAGGTACAG GCTACTACTG ACATTGATAA AGAGCAGACC
    AAGTTAAAAC GATCTTACTC CTCGCCTGAT TTAACGCAAG CCATAAAAGA TGAAGACAAG
    AAGAAACCAA TGCCTCATAT TAACCGTGAA AATAAACCAT CAATAGTCAC TAAAGCTGAA
    ATCACAAAGC TACCAGCATC TAAGATCCGA AGTCTTAATC CTGTGTTTGG CGGGGCCGGT
    CCAGCTCTAA CTGGACTCAG GAACCTTGGA AATACTTGTT ATATGAATTC TATCTTACAG
    TGTCTCTGCA ACACGCCACA TTTAATTGAA TACTTCAATA ATAATTTTTA TCAAGCACAC
    ATCAACAGAT CAAATCTGTT GGGGCATAAG GGTGAAGTGG CTGAGGAATT TGGCTGCATT
    ATGAGAGCTT TATGGACTGG TCAGTACAAA TATATCAGTC CAAAGGACTT TAAGATAACA
    ATTGGAAAAA TTAATGAGCA GTTTGCTGGA TACACCCATC AAGATTCCCA GGAGTTGCTG
    CTGTTTTTAA TGGATGGGTT GCATGAAGAC CTTAATAAGA AAGCAGACAA TAGAAAACGT
    CAAAAAGAAG AAAACAACGA CCATTTAAGT GACTCCCAAG CTGCAGACTC TGCATGGTAC
    AAACACAAGC AACTCAATGA ATCGATCATT GTTGCACTTT TCCAAGGCCA ATTTAAATCA
    ACTGTGCAGT GTCTAACGTG CCAGAAAAAA TCCCGAACAT TTGAGGCGTT CATGTATTTA
    TCTCTGCCAC TTCCATCTAC AAGCAAATGT TCTCTTCAGG ATTGCATTAA AGCATTTGCC
    AAGGAAGAGA AACTCACAGA TAGTAATAGA TTTGATTGCA GTAATTGTAA AACTCAGAGA
    GTTGCTACAA AAAAAATAGA GATTTGGAAG CTTCCTCCTA TTCTTCTGGT GCACTTAAAA
    CGCTTTTCAT TTGAGGGAAG GTGGAGGCAG AAACTCCAGT CATATGTCGA CTTTCCCCTG
    GAATCCTTAG ACCTTTCACA ATACGTCATT GGTCCAAAGA ATGTAAAAAC ATACAATTTA
    TTTGGAGTAT CGAATCACTA TGGAGGTTTG GACGGCGGAC ACTACACAGC TTATTGCAAG
    AATGCGCTGA AACAGCGGTG GTTTAAATTT GATGACCATG ACGTTTCTGA AATCTCCGTA
    TCATCTGTGA AGACTTCCGC AGCCTATATG CTTTTTTACA CTTCTTTGGA TGCCCGAGGT
    CTTGAGATTT CAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947929.1
    CDS734..3451
    Translation

    Target ORF information:

    RefSeq Version XM_018092440.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092440.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGAATG GGGAGAAAAA TGAAGTTCTT TCAAAGCGGC CAGAACCTAC TAATGTGCCT 
    CATGGAGCTA TCACAGTTGA AAAGCTTTTT GAGATGACAA ATGATTATAA TATCAGTATA
    ATTATAATGG ATGTTCGGAG TACAAAAGAC TTTCAGGAGT CTCATATTCG TACTTCAATA
    AGCGTGCCAG AGGAGGCCAT AATCCCAGGA TTTACCTCAA GCAAGATTGA AACTAATCTA
    CCTGATACAT CTAAAGAAAT GTGGAATAAA AGAGGTCAAG TTGATTTTAT TATATTGCTT
    GATTGGTTCA GCTCTGTAAA TAACTTAAAA GTTGGTACAA CACTTCAGAG CTTAAAAGAT
    GCTCTGTATA AGTGGGAAAC AAAAACAATC CTACGGAGTG AACCTTTAGT TTTGGAAGGT
    GGTTATGAAA ATTGGCTTCT TTGTTATCCC ATGTTCACAA CAAATGGGAA AGTGACTGTG
    CCCAGGAAAA GAACACAAGA ATCTGTGTCT TTGTCATTGG ACTTCAGTTA TCCATCTCTA
    GAAGAACCAG CACTTCCACC ACCACCACCA CCCCCTGTAT GCATCAAAGA AAGCAGCTAT
    GAGGATACTA TAAATGCAGT GAAAACTCAA AAAACAGAGG AACCTGTCCA TGTAGTACAA
    AAGGATGACC CTGTACCAGA TGCTCAGACT CCAGATTCTG TTAAAGTCTG TCAGCCACAG
    AAAACTGTGA AAGTTATTCC ACAAATTGAT CGGACAAAGA AACCTTCAGT AAAAGTTACT
    GAAAGTTCCT CAAAGTTTAA ACATGAAGCC AAAGAAAACC AGCCAATACA GAATGGAAGG
    CCTATACCAG ACCGTTCCAC AAAACCATCA CTAGACACTA AATCGGTCAA ATCGGAGATC
    CATGTTCAGT TTGATAAAAA CATAAAGGAA AAGGGTGAAG AAATTAAAGG GAAAGTAGAT
    ATGCATGCAG AAAACAATAT TGATAACACA TGTGCACATG AAGAAAAAGA AAACAAAAAG
    AGGAATGAAG AGAAAGAAAC TGAGATGGAT GAGTTCCACA GAAAGGAGGA GTTAGAAAAA
    AAACAGCCAG TAGTTGCTGG AAAAGAACCT GCTGAAACAA AAAGGCTGGA AAGTAATAAG
    CCTCCCCCTG ATGATAGAAA GAAGAATGGC CATTCTTCTG TTACTCCTCA AGAGCCTTCT
    GCACATAAAA AAGAACATAA TTCTGATGTG ACTGTGAAAG ACAGCTGTGA AGACGACAGA
    CCCAAACGGG AACCTTTAGT TCGAGCAAGA AGTGAGGAGA TGGGTAGAAC TGTGCCAGGA
    ATGCCAGAAG GTTGGGTGAA GTTCCTTGAA CCTGTCACTG GAACCTACCG ATATTATCAT
    TCACCAACAC ACACTGTTCA TATGTATCCT CCTGAAATGG CTCCTTCATC CACCCCTTCA
    ACCACTCCAG CTCAGAAAGC CAAGGTACAG GCTACTACTG ACATTGATAA AGAGCAGACC
    AAGTTAAAAC GATCTTACTC CTCGCCTGAT TTAACGCAAG CCATAAAAGA TGAAGACAAG
    AAGAAACCAA TGCCTCATAT TAACCGTGAA AATAAACCAT CAATAGTCAC TAAAGCTGAA
    ATCACAAAGC TACCAGCATC TAAGATCCGA AGTCTTAATC CTGTGTTTGG CGGGGCCGGT
    CCAGCTCTAA CTGGACTCAG GAACCTTGGA AATACTTGTT ATATGAATTC TATCTTACAG
    TGTCTCTGCA ACACGCCACA TTTAATTGAA TACTTCAATA ATAATTTTTA TCAAGCACAC
    ATCAACAGAT CAAATCTGTT GGGGCATAAG GGTGAAGTGG CTGAGGAATT TGGCTGCATT
    ATGAGAGCTT TATGGACTGG TCAGTACAAA TATATCAGTC CAAAGGACTT TAAGATAACA
    ATTGGAAAAA TTAATGAGCA GTTTGCTGGA TACACCCATC AAGATTCCCA GGAGTTGCTG
    CTGTTTTTAA TGGATGGGTT GCATGAAGAC CTTAATAAGA AAGCAGACAA TAGAAAACGT
    CAAAAAGAAG AAAACAACGA CCATTTAAGT GACTCCCAAG CTGCAGACTC TGCATGGTAC
    AAACACAAGC AACTCAATGA ATCGATCATT GTTGCACTTT TCCAAGGCCA ATTTAAATCA
    ACTGTGCAGT GTCTAACGTG CCAGAAAAAA TCCCGAACAT TTGAGGCGTT CATGTATTTA
    TCTCTGCCAC TTCCATCTAC AAGCAAATGT TCTCTTCAGG ATTGCATTAA AGCATTTGCC
    AAGGAAGAGA AACTCACAGA TAGTAATAGA TTTGATTGCA GTAATTGTAA AACTCAGAGA
    GTTGCTACAA AAAAAATAGA GATTTGGAAG CTTCCTCCTA TTCTTCTGGT GCACTTAAAA
    CGCTTTTCAT TTGAGGGAAG GTGGAGGCAG AAACTCCAGT CATATGTCGA CTTTCCCCTG
    GAATCCTTAG ACCTTTCACA ATACGTCATT GGTCCAAAGA ATGTAAAAAC ATACAATTTA
    TTTGGAGTAT CGAATCACTA TGGAGGTTTG GACGGCGGAC ACTACACAGC TTATTGCAAG
    AATGCGCTGA AACAGCGGTG GTTTAAATTT GATGACCATG ACGTTTCTGA AATCTCCGTA
    TCATCTGTGA AGACTTCCGC AGCCTATATG CTTTTTTACA CTTCTTTGGA TGCCCGAGGT
    CTTGAGATTT CAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55132
    Clone ID Related Accession (Same CDS sequence) XM_018092439.2 , XM_002939624.5 , XM_018092438.2 , XM_012959499.3 , XM_004912609.4
    Accession Version XM_018092439.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin carboxyl-terminal hydrolase 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092439.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCAAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAACGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTTTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947928.2
    CDS275..3454
    Translation

    Target ORF information:

    RefSeq Version XM_018092439.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092439.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCAAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAACGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTTTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55132
    Clone ID Related Accession (Same CDS sequence) XM_018092439.2 , XM_002939624.5 , XM_018092438.2 , XM_012959499.3 , XM_004912609.4
    Accession Version XM_002939624.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin carboxyl-terminal hydrolase 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002939624.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCAAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAACGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTTTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002939670.3
    CDS354..3533
    Translation

    Target ORF information:

    RefSeq Version XM_002939624.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002939624.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCAAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAACGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTTTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55132
    Clone ID Related Accession (Same CDS sequence) XM_018092439.2 , XM_002939624.5 , XM_018092438.2 , XM_012959499.3 , XM_004912609.4
    Accession Version XM_018092438.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin carboxyl-terminal hydrolase 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092438.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCAAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAACGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTTTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947927.2
    CDS327..3506
    Translation

    Target ORF information:

    RefSeq Version XM_018092438.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092438.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCAAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAACGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTTTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55132
    Clone ID Related Accession (Same CDS sequence) XM_018092439.2 , XM_002939624.5 , XM_018092438.2 , XM_012959499.3 , XM_004912609.4
    Accession Version XM_012959499.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin carboxyl-terminal hydrolase 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959499.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCAAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAACGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTTTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814953.2
    CDS291..3470
    Translation

    Target ORF information:

    RefSeq Version XM_012959499.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959499.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCAAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAACGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTTTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55132
    Clone ID Related Accession (Same CDS sequence) XM_018092439.2 , XM_002939624.5 , XM_018092438.2 , XM_012959499.3 , XM_004912609.4
    Accession Version XM_004912609.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin carboxyl-terminal hydrolase 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912609.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCAAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAACGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTTTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004912666.2
    CDS406..3585
    Translation

    Target ORF information:

    RefSeq Version XM_004912609.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004912609.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCAAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAACGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTTTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55133
    Clone ID Related Accession (Same CDS sequence) XM_031899207.1
    Accession Version XM_031899207.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3177bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin carboxyl-terminal hydrolase 8 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCAAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAACGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GGCAGACAAT AGAAAACGTC AAAAAGAAGA AAACAACGAC
    CATTTAAGTG ACTCCCAAGC TGCAGACTCT GCATGGTACA AACACAAGCA ACTCAATGAA
    TCGATCATTG TTGCACTTTT CCAAGGCCAA TTTAAATCAA CTGTGCAGTG TCTAACGTGC
    CAGAAAAAAT CCCGAACATT TGAGGCGTTC ATGTATTTAT CTTTGCCACT TCCATCTACA
    AGCAAATGTT CTCTTCAGGA TTGCATTAAA GCATTTGCCA AGGAAGAGAA ACTCACAGAT
    AGTAATAGAT TTGATTGCAG TAATTGTAAA ACTCAGAGAG TTGCTACAAA AAAAATAGAG
    ATTTGGAAGC TTCCTCCTAT TCTTCTGGTG CACTTAAAAC GCTTTTCATT TGAGGGAAGG
    TGGAGGCAGA AACTCCAGTC ATATGTCGAC TTTCCCCTGG AATCCTTAGA CCTTTCACAA
    TACGTCATTG GTCCAAAGAA TGTAAAAACA TACAATTTAT TTGGAGTATC GAATCACTAT
    GGAGGTTTGG ACGGCGGACA CTACACAGCT TATTGCAAGA ATGCGCTGAA ACAGCGGTGG
    TTTAAATTTG ATGACCATGA CGTTTCTGAA ATCTCCGTAT CATCTGTGAA GACTTCCGCA
    GCCTATATGC TTTTTTACAC TTCTTTGGAT GCCCGAGGTC TTGAGATTTC AGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755067.1
    CDS404..3580
    Translation

    Target ORF information:

    RefSeq Version XM_031899207.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899207.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCAAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAACGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GGCAGACAAT AGAAAACGTC AAAAAGAAGA AAACAACGAC
    CATTTAAGTG ACTCCCAAGC TGCAGACTCT GCATGGTACA AACACAAGCA ACTCAATGAA
    TCGATCATTG TTGCACTTTT CCAAGGCCAA TTTAAATCAA CTGTGCAGTG TCTAACGTGC
    CAGAAAAAAT CCCGAACATT TGAGGCGTTC ATGTATTTAT CTTTGCCACT TCCATCTACA
    AGCAAATGTT CTCTTCAGGA TTGCATTAAA GCATTTGCCA AGGAAGAGAA ACTCACAGAT
    AGTAATAGAT TTGATTGCAG TAATTGTAAA ACTCAGAGAG TTGCTACAAA AAAAATAGAG
    ATTTGGAAGC TTCCTCCTAT TCTTCTGGTG CACTTAAAAC GCTTTTCATT TGAGGGAAGG
    TGGAGGCAGA AACTCCAGTC ATATGTCGAC TTTCCCCTGG AATCCTTAGA CCTTTCACAA
    TACGTCATTG GTCCAAAGAA TGTAAAAACA TACAATTTAT TTGGAGTATC GAATCACTAT
    GGAGGTTTGG ACGGCGGACA CTACACAGCT TATTGCAAGA ATGCGCTGAA ACAGCGGTGG
    TTTAAATTTG ATGACCATGA CGTTTCTGAA ATCTCCGTAT CATCTGTGAA GACTTCCGCA
    GCCTATATGC TTTTTTACAC TTCTTTGGAT GCCCGAGGTC TTGAGATTTC AGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa07224
    Clone ID Related Accession (Same CDS sequence) XM_012959499.2 , XM_002939624.4 , XM_018092439.1 , XM_004912609.3 , XM_018092438.1 , XM_018092437.1
    Accession Version XM_012959499.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin carboxyl-terminal hydrolase 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959499.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCCAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAATGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTCTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814953.1
    CDS304..3483
    Translation

    Target ORF information:

    RefSeq Version XM_012959499.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959499.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCCAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAATGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTCTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa07224
    Clone ID Related Accession (Same CDS sequence) XM_012959499.2 , XM_002939624.4 , XM_018092439.1 , XM_004912609.3 , XM_018092438.1 , XM_018092437.1
    Accession Version XM_002939624.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin carboxyl-terminal hydrolase 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002939624.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCCAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAATGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTCTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002939670.2
    CDS367..3546
    Translation

    Target ORF information:

    RefSeq Version XM_002939624.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002939624.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCCAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAATGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTCTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa07224
    Clone ID Related Accession (Same CDS sequence) XM_012959499.2 , XM_002939624.4 , XM_018092439.1 , XM_004912609.3 , XM_018092438.1 , XM_018092437.1
    Accession Version XM_018092439.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin carboxyl-terminal hydrolase 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCCAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAATGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTCTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947928.1
    CDS289..3468
    Translation

    Target ORF information:

    RefSeq Version XM_018092439.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092439.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCCAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAATGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTCTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa07224
    Clone ID Related Accession (Same CDS sequence) XM_012959499.2 , XM_002939624.4 , XM_018092439.1 , XM_004912609.3 , XM_018092438.1 , XM_018092437.1
    Accession Version XM_004912609.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin carboxyl-terminal hydrolase 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912609.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCCAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAATGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTCTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004912666.1
    CDS419..3598
    Translation

    Target ORF information:

    RefSeq Version XM_004912609.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004912609.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCCAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAATGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTCTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa07224
    Clone ID Related Accession (Same CDS sequence) XM_012959499.2 , XM_002939624.4 , XM_018092439.1 , XM_004912609.3 , XM_018092438.1 , XM_018092437.1
    Accession Version XM_018092438.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin carboxyl-terminal hydrolase 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCCAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAATGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTCTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947927.1
    CDS341..3520
    Translation

    Target ORF information:

    RefSeq Version XM_018092438.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092438.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCCAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAATGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTCTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa07224
    Clone ID Related Accession (Same CDS sequence) XM_012959499.2 , XM_002939624.4 , XM_018092439.1 , XM_004912609.3 , XM_018092438.1 , XM_018092437.1
    Accession Version XM_018092437.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin carboxyl-terminal hydrolase 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCCAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAATGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTCTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947926.1
    CDS356..3535
    Translation

    Target ORF information:

    RefSeq Version XM_018092437.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092437.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCCAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAATGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GAAAGCAGAC AATAGAAAAC GTCAAAAAGA AGAAAACAAC
    GACCATTTAA GTGACTCCCA AGCTGCAGAC TCTGCATGGT ACAAACACAA GCAACTCAAT
    GAATCGATCA TTGTTGCACT TTTCCAAGGC CAATTTAAAT CAACTGTGCA GTGTCTAACG
    TGCCAGAAAA AATCCCGAAC ATTTGAGGCG TTCATGTATT TATCTCTGCC ACTTCCATCT
    ACAAGCAAAT GTTCTCTTCA GGATTGCATT AAAGCATTTG CCAAGGAAGA GAAACTCACA
    GATAGTAATA GATTTGATTG CAGTAATTGT AAAACTCAGA GAGTTGCTAC AAAAAAAATA
    GAGATTTGGA AGCTTCCTCC TATTCTTCTG GTGCACTTAA AACGCTTTTC ATTTGAGGGA
    AGGTGGAGGC AGAAACTCCA GTCATATGTC GACTTTCCCC TGGAATCCTT AGACCTTTCA
    CAATACGTCA TTGGTCCAAA GAATGTAAAA ACATACAATT TATTTGGAGT ATCGAATCAC
    TATGGAGGTT TGGACGGCGG ACACTACACA GCTTATTGCA AGAATGCGCT GAAACAGCGG
    TGGTTTAAAT TTGATGACCA TGACGTTTCT GAAATCTCCG TATCATCTGT GAAGACTTCC
    GCAGCCTATA TGCTTTTTTA CACTTCTTTG GATGCCCGAG GTCTTGAGAT TTCAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa07225
    Clone ID Related Accession (Same CDS sequence) XM_012959500.2
    Accession Version XM_012959500.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3177bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin carboxyl-terminal hydrolase 8 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959500.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCCAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAATGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GGCAGACAAT AGAAAACGTC AAAAAGAAGA AAACAACGAC
    CATTTAAGTG ACTCCCAAGC TGCAGACTCT GCATGGTACA AACACAAGCA ACTCAATGAA
    TCGATCATTG TTGCACTTTT CCAAGGCCAA TTTAAATCAA CTGTGCAGTG TCTAACGTGC
    CAGAAAAAAT CCCGAACATT TGAGGCGTTC ATGTATTTAT CTCTGCCACT TCCATCTACA
    AGCAAATGTT CTCTTCAGGA TTGCATTAAA GCATTTGCCA AGGAAGAGAA ACTCACAGAT
    AGTAATAGAT TTGATTGCAG TAATTGTAAA ACTCAGAGAG TTGCTACAAA AAAAATAGAG
    ATTTGGAAGC TTCCTCCTAT TCTTCTGGTG CACTTAAAAC GCTTTTCATT TGAGGGAAGG
    TGGAGGCAGA AACTCCAGTC ATATGTCGAC TTTCCCCTGG AATCCTTAGA CCTTTCACAA
    TACGTCATTG GTCCAAAGAA TGTAAAAACA TACAATTTAT TTGGAGTATC GAATCACTAT
    GGAGGTTTGG ACGGCGGACA CTACACAGCT TATTGCAAGA ATGCGCTGAA ACAGCGGTGG
    TTTAAATTTG ATGACCATGA CGTTTCTGAA ATCTCCGTAT CATCTGTGAA GACTTCCGCA
    GCCTATATGC TTTTTTACAC TTCTTTGGAT GCCCGAGGTC TTGAGATTTC AGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814954.1
    CDS366..3542
    Translation

    Target ORF information:

    RefSeq Version XM_012959500.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin specific peptidase 8 (usp8), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959500.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTACAG TATCAGCACT TCCCAAAGAA CTGTATCTCT CTAGCTCTCT ATCTGATCTT 
    AAGAAGAAAG CTGAAGTTAA ACCTGACAAG ACTAGCACTA AAAGCTATGT TTCCTCTGCT
    TGTAAAATAT TTCAAAAAGC AGAAGAATGC AGACTAGAAG GAGATGAAGA AAAAGCCTAT
    GTCTTTTATA TGAAATATTT ATTTGTGTTT GAACTCCTTA AAAAAAGATC AGATTTTAAG
    CAGAATCAGG ACTATTATAA GTCCTTACTT GGCCCCTCCA CTATCTTAAA ATCTGTTGAA
    GAAGCGGAAA AACTCTCAGA AAGCCTTAAA AATAGATATG AAGAAGCTGA AGTTCGAAAA
    AAATTAGAGG AAAGGGAAAG ACAAGAAGAC CTGAAGAAAA AGGAGGGAAA ACTATTTCCC
    AAAGACTTTC CTGAAGATGT ACCCAAGCCT AATCAAAATG AAATGGTGAA TGGGGAGAAA
    AATGAAGTTC TTTCAAAGCG GCCAGAACCT ACTAATGTGC CTCATGGAGC TATCACAGTT
    GAAAAGCTTT TTGAGATGAC AAATGATTAT AATATCAGTA TAATTATAAT GGATGTTCGG
    AGTACAAAAG ACTTTCAGGA GTCTCATATT CGTACTTCAA TAAGCGTGCC AGAGGAGGCC
    ATAATCCCAG GATTTACCTC AAGCAAGATT GAAACTAATC TACCTGATAC ATCTAAAGAA
    ATGTGGAATA AAAGAGGTCA AGTTGATTTT ATTATATTGC TTGATTGGTT CAGCTCTGTA
    AATAACTTAA AAGTTGGTAC AACACTTCAG AGCTTAAAAG ATGCTCTGTA TAAGTGGGAA
    ACAAAAACAA TCCTACGGAG TGAACCTTTA GTTTTGGAAG GTGGTTATGA AAATTGGCTT
    CTTTGTTATC CCATGTTCAC AACAAATGGG AAAGTGACTG TGCCCAGGAA AAGAACACAA
    GAATCTGTGT CTTTGTCATT GGACTTCAGT TATCCATCTC TAGAAGAACC AGCACTTCCA
    CCACCACCAC CACCCCCTGT ATGCATCAAA GAAAGCAGCT ATGAGGATAC TATAAATGCA
    GTGAAAACTC AAAAAACAGA GGAACCTGTC CATGTAGTAC AAAAGGATGA CCCTGTACCA
    GATGCTCAGA CTCCAGATTC TGTTAAAGTC TGTCAGCCAC AGAAAACTGT GAAAGTTATT
    CCACAAATTG ATCGGACAAA GAAACCTTCA GTAAAAGTTA CTGAAAGTTC CTCAAAGTTT
    AAACATGAAG CCAAAGAAAA CCAGCCAATA CAGAATGGAA GGCCTATACC AGACCGTTCC
    ACAAAACCAT CACTAGACAC TAAATCGGTC AAATCGGAGA TCCATGTTCA GTTTGATAAA
    AACATAAAGG AAAAGGGTGA AGAAATTAAA GGGAAAGTAG ATATGCATGC AGAAAACAAT
    ATTGATAACA CATGTGCACA TGAAGAAAAA GAAAACAAAA AGAGGAATGA AGAGAAAGAA
    ACTGAGATGG ATGAGTTCCA CAGAAAGGAG GAGTTAGAAA AAAAACAGCC AGTAGTTGCT
    GGAAAAGAAC CTGCTGAAAC AAAAAGGCTG GAAAGTAATA AGCCTCCCCC TGATGATAGA
    AAGAAGAATG GCCATTCTTC TGTTACTCCT CAAGAGCCTT CTGCACATAA AAAAGAACAT
    AATTCTGATG TGACTGTGAA AGACAGCTGT GAAGACGACA GACCCAAACG GGAACCTTTA
    GTTCGAGCAA GAAGTGAGGA GATGGGTAGA ACTGTGCCAG GAATGCCAGA AGGTTGGGTG
    AAGTTCCTTG AACCTGTCAC TGGAACCTAC CGATATTATC ATTCACCAAC ACACACTGTT
    CATATGTATC CTCCTGAAAT GGCTCCTTCA TCCACCCCTT CAACCACTCC AGCTCAGAAA
    GCCAAGGTAC AGGCTACTAC TGACATTGAT AAAGAGCAGA CCAAGTTAAA ACGATCTTAC
    TCCTCGCCTG ATTTAACGCA AGCCATAAAA GATGAAGACA AGAAGAAACC AATGCCTCAT
    ATTAACCGTG AAAATAAACC ATCAATAGTC ACTAAAGCTG AAATCACAAA GCTACCAGCA
    TCTAAGATCC GAAGTCTTAA TCCTGTGTTT GGCGGGGCCG GTCCAGCTCT AACTGGACTC
    AGGAACCTTG GAAATACTTG TTATATGAAT TCTATCTTAC AGTGTCTCTG CAACACGCCA
    CATTTAATTG AATACTTCAA TAATAATTTT TATCAAGCAC ACATCAACAG ATCAAATCTG
    TTGGGGCATA AGGGTGAAGT GGCTGAGGAA TTTGGCTGCA TTATGAGAGC TTTATGGACT
    GGTCAGTACA AATATATCAG TCCAAAGGAC TTTAAGATAA CAATTGGAAA AATTAATGAG
    CAGTTTGCTG GATACACCCA TCAAGATTCC CAGGAGTTGC TGCTGTTTTT AATGGATGGG
    TTGCATGAAG ACCTTAATAA GGCAGACAAT AGAAAACGTC AAAAAGAAGA AAACAACGAC
    CATTTAAGTG ACTCCCAAGC TGCAGACTCT GCATGGTACA AACACAAGCA ACTCAATGAA
    TCGATCATTG TTGCACTTTT CCAAGGCCAA TTTAAATCAA CTGTGCAGTG TCTAACGTGC
    CAGAAAAAAT CCCGAACATT TGAGGCGTTC ATGTATTTAT CTCTGCCACT TCCATCTACA
    AGCAAATGTT CTCTTCAGGA TTGCATTAAA GCATTTGCCA AGGAAGAGAA ACTCACAGAT
    AGTAATAGAT TTGATTGCAG TAATTGTAAA ACTCAGAGAG TTGCTACAAA AAAAATAGAG
    ATTTGGAAGC TTCCTCCTAT TCTTCTGGTG CACTTAAAAC GCTTTTCATT TGAGGGAAGG
    TGGAGGCAGA AACTCCAGTC ATATGTCGAC TTTCCCCTGG AATCCTTAGA CCTTTCACAA
    TACGTCATTG GTCCAAAGAA TGTAAAAACA TACAATTTAT TTGGAGTATC GAATCACTAT
    GGAGGTTTGG ACGGCGGACA CTACACAGCT TATTGCAAGA ATGCGCTGAA ACAGCGGTGG
    TTTAAATTTG ATGACCATGA CGTTTCTGAA ATCTCCGTAT CATCTGTGAA GACTTCCGCA
    GCCTATATGC TTTTTTACAC TTCTTTGGAT GCCCGAGGTC TTGAGATTTC AGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.