Mccc1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mccc1
Entrez Gene ID	294972
Full Name	methylcrotonoyl-CoA carboxylase 1
General protein information	Preferred Names methylcrotonoyl-CoA carboxylase 1 Names methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial 3-methylcrotonyl-CoA carboxylase 1 3-methylcrotonyl-CoA carboxylase biotin-containing subunit 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha MCCase subunit alpha methylcrotonoyl-CoA carboxylase 1 (alpha) methylcrotonoyl-Coenzyme A carboxylase 1 (alpha)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q25

mRNA and Protein(s)

mRNA	Protein	Name
XM_017590738.1	XP_017446227.1	methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X1
NM_001009653.1	NP_001009653.1	methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mccc1	NP_001009653.1
Gallus gallus (chicken)	MCCC1	NP_001006551.1
Drosophila melanogaster (fruit fly)	CG2118	NP_001247391.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010228	XP_319416.4
Neurospora crassa	NCU00591	XP_965731.1
Oryza sativa (rice)	Os12g0605800	NP_001067226.1
Mus musculus (house mouse)	Mccc1	NP_076133.3
Danio rerio (zebrafish)	mccc1	NP_001038380.1
Magnaporthe oryzae (rice blast fungus)	MGG_10320	XP_003720502.1
Xenopus tropicalis (tropical clawed frog)	mccc1	NP_001006691.1
Homo sapiens (human)	MCCC1	NP_064551.3
	LOC708258	XP_001096717.1
Pan troglodytes (chimpanzee)	MCCC1	XP_516898.3
Canis lupus familiaris (dog)	MCCC1	XP_535817.2
Bos taurus (cattle)	MCCC1	NP_001192687.1
Caenorhabditis elegans (roundworm)	F32B6.2	NP_501777.2
Arabidopsis thaliana (thale cress)	MCCA	NP_849583.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression, purification, characterization of human 3-methylcrotonyl-CoA carboxylase (MCCC).
Chu CH, Cheng D
Protein expression and purification53(2)421-7(2007 Jun)

Mitochondrial targeting signals and mature peptides of 3-methylcrotonyl-CoA carboxylase.
Stadler SC, Polanetz R, Meier S, Mayerhofer PU, Herrmann JM, Anslinger K, Roscher AA, R?schinger W, Holzinger A
Biochemical and biophysical research communications334(3)939-46(2005 Sep)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

Proteomic analysis of the mouse liver mitochondrial inner membrane.
Da Cruz S, Xenarios I, Langridge J, Vilbois F, Parone PA, Martinou JC
The Journal of biological chemistry278(42)41566-71(2003 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mccc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mccc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42328	NM_001009653.1 Latest version!	Rattus norvegicus methylcrotonoyl-CoA carboxylase 1 (Mccc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa59669	XM_017590738.1 Latest version!	Rattus norvegicus methylcrotonoyl-CoA carboxylase 1 (Mccc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mccc1 ( NM_001009653.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001009653.1 Rattus norvegicus methylcrotonoyl-CoA carboxylase 1 (Mccc1), mRNA.

Price & Availability↑

CloneID	ORa42328
Clone ID Related Accession (Same CDS sequence)	NM_001009653.1
Accession Version	NM_001009653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC088473.1. On Jan 13, 2005 this sequence version replaced XM_215554.2. ##Evidence-Data-START## Transcript exon combination :: BC088473.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGCGG CGGCTCTGTT GGCTGCGGTG GACAGGAACC AGTTGCGGCG GGTCCCGATC 
CTGCTGCTGC AGCCGAGGGA ATGGCCTTGG AAGCACAGAA CCGTGAAATA TGGAACAACC 
CCAGGAGGAA GCATCACCAA GGTCCTCATT GCAAACAGAG GAGAAATTGC CTGCAGGGTG 
ATACGAACAG CCAGAAAAAT GGGTGTACAA TCTGTGGCTG TTTACAGCGA GGCCGACAGG 
AATTCCATGC ATGTAGATAT GGCAGACGAG GCGTATTCCA TTGGCCCTGC GCCATCTCAG 
CAGAGCTACC TCGCAATGGA GAAAATCATC CAAGTGGCCA AGAGCTCTGC AGCACAGGCC 
ATCCATCCAG GATATGGTTT TCTTTCAGAA AATATGGAGT TTGCTGAATT TTGTAAGCAA 
GAAGGAATTA TTTTTATAGG TCCTCCTTCA ACTGCAATTA GAGACATGGG TATAAAGAGC 
ACATCCAAGT CCATCATGGC TGCCGCTGGA GTACCTGTTG TGGAAGGTTA CCATGGCAAC 
GACCAATCTG ATGAGTGCCT GAAAGAGCAT GCAGGGAAAA TCGGGTATCC TGTGATGATC 
AAAGCCATCC GTGGTGGAGG AGGAAAAGGC ATGAGGATCA TTAGGTCAGA AAAAGAATTC 
CAAGAGCAGC TAGAATCAGC CCGGAGGGAG GCAAAGAAGT CTTTCAACGA TGACGCAATG 
CTGATCGAGA AGTTCGTGGA CACGCCAAGG CATGTGGAAG TCCAGGTGTT TGGTGATCAC 
CATGGCAATG CAGTGTACTT GTTTGAAAGA GACTGTAGTG TGCAGAGGCG ACATCAGAAG 
ATCATTGAGG AGGCCCCAGC GCCTGGTATT GATCCTGAAG TAAGAAGGAG GCTGGGAGAG 
GCTGCAGTCA GAGCTGCGAA GGCTGTCAAC TATGTGGGAG CAGGAACTGT GGAATTCATT 
ATGGACTCAA AGCACAATTT TTATTTCATG GAGATGAACA CAAGGCTACA AGTGGAGCAT 
CCGGTCACTG AGATGATCAC GGGGACTGAC TTGGTGGAGT GGCAGCTCAG GATTGCAGCA 
GGGGAAAAGA TCCCTTTGAG CCAGGAAGAA ATCCCTCTGC AGGGCCATGC CTTTGAGGCC 
AGAATCTATG CAGAGGATCC TGACAATAAC TTCATGCCAG GAGCTGGACC ATTAGTTCAC 
CTCTCTACCC CTCCGCCAGA CATGTCTACA AGGATCGAAA CTGGAGTACG GCAAGGAGAT 
GAAGTCTCGG TGCATTACGA CCCCATGATC GCAAAGCTGG TGGTGTGGGC CTCAGATCGC 
CAGTCAGCCC TGTCAAAACT GAGATACAGT CTTCATCAGT ACAACATTGT TGGCTTGCGC 
ACCAATGTGG ACTTCCTGCT CCGGCTCTCC GGCCACTCAG AGTTTGAAGC TGGGAATGTA 
CACACTGACT TCATCCCTCA ACACCACAAG GACCTGCTGC CCACTCACAG CACCATAGCC 
AAGGAATCTG TGTGCCAGGC AGCCTTGGGG CTTATCCTCA AAGAGAAAGA AATGACCAGC 
GCTTTTAAAC TCCACACTCA AGATCAATTC TCTCCGTTTT CATTCAGCAG TGGGAGAAGA 
CTGAATATCT CTTACACCAG GAACATGACT TTAAGAAGTG GTAAAAACGA TATAATCATA 
GCTGTGACGT ATAACCGAGA TGGGTCATAT GACATGCAGA TTGAAAATAA GTTATTCCGA 
GTCCTGGGCG ATCTTTCCAA TGAGGATGGC TATACCTACC TGAAGTCTTC TGTTAATGGA 
GTAGCCAGTA AATCCAAGTT CATCCTCCTG GACAACACCA TCTACCTGTT TTCTATGGAA 
GGCAGTATTG AAGTTGGCAT TCCAGTACCC AAGTACTTGT CTCCAGTGAG TGCAGAAGGA 
ACTCAGGGAG GCACCATCGC TCCCATGACT GGAACCATTG AAAAGGTGTT CGTGAAGGCT 
GGAGACAGAG TAAAGGCTGG AGACGCTCTG ATGGTTATGA TCGCCATGAA GATGGAGCAC 
ACCATAAAAG CCCCAAAGGA CGGCAGGATA AAAAAGGTGT TTTTCAGTGA AGGAGCCCAA 
GCCAACAGAC ATGCACCTTT AGTGGAGTTT GAAGAGGAGG AAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009653.1
CDS	19..2166
Translation	MAAAALLAAVDRNQLRRVPILLLQPREWPWKHRTVKYGTTPGGSITKVLIANRGEIACRVIRTARKMGVQSVAVYSEADRNSMHVDMADEAYSIGPAPSQQSYLAMEKIIQVAKSSAAQAIHPGYGFLSENMEFAEFCKQEGIIFIGPPSTAIRDMGIKSTSKSIMAAAGVPVVEGYHGNDQSDECLKEHAGKIGYPVMIKAIRGGGGKGMRIIRSEKEFQEQLESARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQRRHQKIIEEAPAPGIDPEVRRRLGEAAVRAAKAVNYVGAGTVEFIMDSKHNFYFMEMNTRLQVEHPVTEMITGTDLVEWQLRIAAGEKIPLSQEEIPLQGHAFEARIYAEDPDNNFMPGAGPLVHLSTPPPDMSTRIETGVRQGDEVSVHYDPMIAKLVVWASDRQSALSKLRYSLHQYNIVGLRTNVDFLLRLSGHSEFEAGNVHTDFIPQHHKDLLPTHSTIAKESVCQAALGLILKEKEMTSAFKLHTQDQFSPFSFSSGRRLNISYTRNMTLRSGKNDIIIAVTYNRDGSYDMQIENKLFRVLGDLSNEDGYTYLKSSVNGVASKSKFILLDNTIYLFSMEGSIEVGIPVPKYLSPVSAEGTQGGTIAPMTGTIEKVFVKAGDRVKAGDALMVMIAMKMEHTIKAPKDGRIKKVFFSEGAQANRHAPLVEFEEEEV

Target ORF information:

RefSeq Version	NM_001009653.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus methylcrotonoyl-CoA carboxylase 1 (Mccc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009653.1

ORF Insert Sequence:

ATGGCGGCGG CGGCTCTGTT GGCTGCGGTG GACAGGAACC AGTTGCGGCG GGTCCCGATC 
CTGCTGCTGC AGCCGAGGGA ATGGCCTTGG AAGCACAGAA CCGTGAAATA TGGAACAACC 
CCAGGAGGAA GCATCACCAA GGTCCTCATT GCAAACAGAG GAGAAATTGC CTGCAGGGTG 
ATACGAACAG CCAGAAAAAT GGGTGTACAA TCTGTGGCTG TTTACAGCGA GGCCGACAGG 
AATTCCATGC ATGTAGATAT GGCAGACGAG GCGTATTCCA TTGGCCCTGC GCCATCTCAG 
CAGAGCTACC TCGCAATGGA GAAAATCATC CAAGTGGCCA AGAGCTCTGC AGCACAGGCC 
ATCCATCCAG GATATGGTTT TCTTTCAGAA AATATGGAGT TTGCTGAATT TTGTAAGCAA 
GAAGGAATTA TTTTTATAGG TCCTCCTTCA ACTGCAATTA GAGACATGGG TATAAAGAGC 
ACATCCAAGT CCATCATGGC TGCCGCTGGA GTACCTGTTG TGGAAGGTTA CCATGGCAAC 
GACCAATCTG ATGAGTGCCT GAAAGAGCAT GCAGGGAAAA TCGGGTATCC TGTGATGATC 
AAAGCCATCC GTGGTGGAGG AGGAAAAGGC ATGAGGATCA TTAGGTCAGA AAAAGAATTC 
CAAGAGCAGC TAGAATCAGC CCGGAGGGAG GCAAAGAAGT CTTTCAACGA TGACGCAATG 
CTGATCGAGA AGTTCGTGGA CACGCCAAGG CATGTGGAAG TCCAGGTGTT TGGTGATCAC 
CATGGCAATG CAGTGTACTT GTTTGAAAGA GACTGTAGTG TGCAGAGGCG ACATCAGAAG 
ATCATTGAGG AGGCCCCAGC GCCTGGTATT GATCCTGAAG TAAGAAGGAG GCTGGGAGAG 
GCTGCAGTCA GAGCTGCGAA GGCTGTCAAC TATGTGGGAG CAGGAACTGT GGAATTCATT 
ATGGACTCAA AGCACAATTT TTATTTCATG GAGATGAACA CAAGGCTACA AGTGGAGCAT 
CCGGTCACTG AGATGATCAC GGGGACTGAC TTGGTGGAGT GGCAGCTCAG GATTGCAGCA 
GGGGAAAAGA TCCCTTTGAG CCAGGAAGAA ATCCCTCTGC AGGGCCATGC CTTTGAGGCC 
AGAATCTATG CAGAGGATCC TGACAATAAC TTCATGCCAG GAGCTGGACC ATTAGTTCAC 
CTCTCTACCC CTCCGCCAGA CATGTCTACA AGGATCGAAA CTGGAGTACG GCAAGGAGAT 
GAAGTCTCGG TGCATTACGA CCCCATGATC GCAAAGCTGG TGGTGTGGGC CTCAGATCGC 
CAGTCAGCCC TGTCAAAACT GAGATACAGT CTTCATCAGT ACAACATTGT TGGCTTGCGC 
ACCAATGTGG ACTTCCTGCT CCGGCTCTCC GGCCACTCAG AGTTTGAAGC TGGGAATGTA 
CACACTGACT TCATCCCTCA ACACCACAAG GACCTGCTGC CCACTCACAG CACCATAGCC 
AAGGAATCTG TGTGCCAGGC AGCCTTGGGG CTTATCCTCA AAGAGAAAGA AATGACCAGC 
GCTTTTAAAC TCCACACTCA AGATCAATTC TCTCCGTTTT CATTCAGCAG TGGGAGAAGA 
CTGAATATCT CTTACACCAG GAACATGACT TTAAGAAGTG GTAAAAACGA TATAATCATA 
GCTGTGACGT ATAACCGAGA TGGGTCATAT GACATGCAGA TTGAAAATAA GTTATTCCGA 
GTCCTGGGCG ATCTTTCCAA TGAGGATGGC TATACCTACC TGAAGTCTTC TGTTAATGGA 
GTAGCCAGTA AATCCAAGTT CATCCTCCTG GACAACACCA TCTACCTGTT TTCTATGGAA 
GGCAGTATTG AAGTTGGCAT TCCAGTACCC AAGTACTTGT CTCCAGTGAG TGCAGAAGGA 
ACTCAGGGAG GCACCATCGC TCCCATGACT GGAACCATTG AAAAGGTGTT CGTGAAGGCT 
GGAGACAGAG TAAAGGCTGG AGACGCTCTG ATGGTTATGA TCGCCATGAA GATGGAGCAC 
ACCATAAAAG CCCCAAAGGA CGGCAGGATA AAAAAGGTGT TTTTCAGTGA AGGAGCCCAA 
GCCAACAGAC ATGCACCTTT AGTGGAGTTT GAAGAGGAGG AAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mccc1 ( XM_017590738.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446227.1 Rattus norvegicus methylcrotonoyl-CoA carboxylase 1 (Mccc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa59669
Clone ID Related Accession (Same CDS sequence)	XM_017590738.1
Accession Version	XM_017590738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGTAC AATCTGTGGC TGTTTACAGC GAGGCCGACA GGAATTCCAT GCATGTAGAT 
ATGGCAGACG AGGCGTATTC CATTGGCCCT GCGCCATCTC AGCAGAGCTA CCTCGCAATG 
GAGAAAATCA TCCAAGTGGC CAAGAGCTCT GCAGCACAGG CCATCCATCC AGGATATGGT 
TTTCTTTCAG AAAATATGGA GTTTGCTGAA CTTTGTAAGC AAGAAGGAAT TATTTTTATA 
GGTCCTCCTT CAACTGCAAT TAGAGACATG GGTATAAAGA GCACATCCAA GTCCATCATG 
GCTGCCGCTG GAGTACCTGT TGTGGAAGGT TACCATGGCA ACGACCAATC TGATGAGTGC 
CTGAAAGAGC ATGCAGGGAA AATCGGGTAT CCTGTGATGA TCAAAGCCAT CCGTGGTGGA 
GGAGGAAAAG GCATGAGGAT CATTAGGTCA GAAAAAGAAT TCCAAGAGCA GCTAGAATCA 
GCCCGGAGGG AGGCAAAGAA GTCTTTCAAC GATGACGCAA TGCTGATCGA GAAGTTCGTG 
GACACGCCAA GGCATGTGGA AGTCCAGGTG TTTGGTGATC ACCATGGCAA TGCAGTGTAC 
TTGTTTGAAA GAGACTGTAG TGTGCAGAGG CGACATCAGA AGATCATTGA GGAGGCCCCA 
GCGCCTGGTA TTGATCCTGA AGTAAGAAGG AGGCTGGGAG AGGCTGCAGT CAGAGCTGCG 
AAGGCTGTCA ACTATGTGGG AGCAGGAACT GTGGAATTCA TTATGGACTC AAAGCACAAT 
TTTTATTTCA TGGAGATGAA CACAAGGCTA CAAGTGGAGC ATCCGGTCAC TGAGATGATC 
ACGGGGACTG ACTTGGTGGA GTGGCAGCTC AGGATTGCAG CAGGGGAAAA GATCCCTTTG 
AGCCAGGAAG AAATCCCTCT GCAGGGCCAT GCCTTTGAGG CCAGAATCTA TGCAGAGGAT 
CCTGACAATA ACTTCATGCC AGGAGCTGGA CCATTAGTTC ACCTCTCTAC CCCTCCGCCA 
GACATGTCTA CAAGGATCGA AACTGGAGTA CGGCAAGGAG ATGAAGTCTC GGTGCATTAC 
GACCCCATGA TCGCAAAGCT GGTGGTGTGG GCCTCAGATC GCCAGTCAGC CCTGTCAAAA 
CTGAGATACA GTCTTCATCA GTACAACATT GTTGGCTTGC GCACCAATGT GGACTTCCTG 
CTCCGGCTCT CCGGCCACTC AGAGTTTGAA GCTGGGAATG TACACACTGA CTTCATCCCT 
CAACACCACA AGGACCTGCT GCCCACTCAC AGCACCATAG CCAAGGAATC TGTGTGCCAG 
GCAGCCTTGG GGCTTATCCT CAAAGAGAAA GAAATGACCA GCGCTTTTAA ACTCCACACT 
CAAGATCAAT TCTCTCCGTT TTCATTCAGC AGTGGGAGAA GACTGAATAT CTCTTACACC 
AGGAACATGA CTTTAAGAAG TGGTAAAAAC GATATAATCA TAGCTGTGAC GTATAACCGA 
GATGGGTCAT ATGACATGCA GATTGAAAAT AAGTTATTCC GAGTCCTGGG CGATCTTTCC 
AATGAGGATG GCTATACCTA CCTGAAGTCT TCTGTTAATG GAGTAGCCAG TAAATCCAAG 
TTCATCCTCC TGGACAACAC CATCTACCTG TTTTCTATGG AAGGCAGTAT TGAAGTTGGC 
ATTCCAGTAC CCAAGTACTT GTCTCCAGTG AGTGCAGAAG GAACTCAGGG AGGCACCATC 
GCTCCCATGA CTGGAACCAT TGAAAAGGTG TTCGTGAAGG CTGGAGACAG AGTAAAGGCT 
GGAGACGCTC TGATGGTTAT GATCGCCATG AAGATGGAGC ACACCATAAA AGCCCCAAAG 
GACGGCAGGA TAAAAAAGGT GTTTTTCAGT GAAGGAGCCC AAGCCAACAG ACATGCACCT 
TTAGTGGAGT TTGAAGAGGA GGAAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446227.1
CDS	214..2163
Translation	MGVQSVAVYSEADRNSMHVDMADEAYSIGPAPSQQSYLAMEKIIQVAKSSAAQAIHPGYGFLSENMEFAELCKQEGIIFIGPPSTAIRDMGIKSTSKSIMAAAGVPVVEGYHGNDQSDECLKEHAGKIGYPVMIKAIRGGGGKGMRIIRSEKEFQEQLESARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQRRHQKIIEEAPAPGIDPEVRRRLGEAAVRAAKAVNYVGAGTVEFIMDSKHNFYFMEMNTRLQVEHPVTEMITGTDLVEWQLRIAAGEKIPLSQEEIPLQGHAFEARIYAEDPDNNFMPGAGPLVHLSTPPPDMSTRIETGVRQGDEVSVHYDPMIAKLVVWASDRQSALSKLRYSLHQYNIVGLRTNVDFLLRLSGHSEFEAGNVHTDFIPQHHKDLLPTHSTIAKESVCQAALGLILKEKEMTSAFKLHTQDQFSPFSFSSGRRLNISYTRNMTLRSGKNDIIIAVTYNRDGSYDMQIENKLFRVLGDLSNEDGYTYLKSSVNGVASKSKFILLDNTIYLFSMEGSIEVGIPVPKYLSPVSAEGTQGGTIAPMTGTIEKVFVKAGDRVKAGDALMVMIAMKMEHTIKAPKDGRIKKVFFSEGAQANRHAPLVEFEEEEV

Target ORF information:

RefSeq Version	XM_017590738.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus methylcrotonoyl-CoA carboxylase 1 (Mccc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590738.1

ORF Insert Sequence:

ATGGGTGTAC AATCTGTGGC TGTTTACAGC GAGGCCGACA GGAATTCCAT GCATGTAGAT 
ATGGCAGACG AGGCGTATTC CATTGGCCCT GCGCCATCTC AGCAGAGCTA CCTCGCAATG 
GAGAAAATCA TCCAAGTGGC CAAGAGCTCT GCAGCACAGG CCATCCATCC AGGATATGGT 
TTTCTTTCAG AAAATATGGA GTTTGCTGAA CTTTGTAAGC AAGAAGGAAT TATTTTTATA 
GGTCCTCCTT CAACTGCAAT TAGAGACATG GGTATAAAGA GCACATCCAA GTCCATCATG 
GCTGCCGCTG GAGTACCTGT TGTGGAAGGT TACCATGGCA ACGACCAATC TGATGAGTGC 
CTGAAAGAGC ATGCAGGGAA AATCGGGTAT CCTGTGATGA TCAAAGCCAT CCGTGGTGGA 
GGAGGAAAAG GCATGAGGAT CATTAGGTCA GAAAAAGAAT TCCAAGAGCA GCTAGAATCA 
GCCCGGAGGG AGGCAAAGAA GTCTTTCAAC GATGACGCAA TGCTGATCGA GAAGTTCGTG 
GACACGCCAA GGCATGTGGA AGTCCAGGTG TTTGGTGATC ACCATGGCAA TGCAGTGTAC 
TTGTTTGAAA GAGACTGTAG TGTGCAGAGG CGACATCAGA AGATCATTGA GGAGGCCCCA 
GCGCCTGGTA TTGATCCTGA AGTAAGAAGG AGGCTGGGAG AGGCTGCAGT CAGAGCTGCG 
AAGGCTGTCA ACTATGTGGG AGCAGGAACT GTGGAATTCA TTATGGACTC AAAGCACAAT 
TTTTATTTCA TGGAGATGAA CACAAGGCTA CAAGTGGAGC ATCCGGTCAC TGAGATGATC 
ACGGGGACTG ACTTGGTGGA GTGGCAGCTC AGGATTGCAG CAGGGGAAAA GATCCCTTTG 
AGCCAGGAAG AAATCCCTCT GCAGGGCCAT GCCTTTGAGG CCAGAATCTA TGCAGAGGAT 
CCTGACAATA ACTTCATGCC AGGAGCTGGA CCATTAGTTC ACCTCTCTAC CCCTCCGCCA 
GACATGTCTA CAAGGATCGA AACTGGAGTA CGGCAAGGAG ATGAAGTCTC GGTGCATTAC 
GACCCCATGA TCGCAAAGCT GGTGGTGTGG GCCTCAGATC GCCAGTCAGC CCTGTCAAAA 
CTGAGATACA GTCTTCATCA GTACAACATT GTTGGCTTGC GCACCAATGT GGACTTCCTG 
CTCCGGCTCT CCGGCCACTC AGAGTTTGAA GCTGGGAATG TACACACTGA CTTCATCCCT 
CAACACCACA AGGACCTGCT GCCCACTCAC AGCACCATAG CCAAGGAATC TGTGTGCCAG 
GCAGCCTTGG GGCTTATCCT CAAAGAGAAA GAAATGACCA GCGCTTTTAA ACTCCACACT 
CAAGATCAAT TCTCTCCGTT TTCATTCAGC AGTGGGAGAA GACTGAATAT CTCTTACACC 
AGGAACATGA CTTTAAGAAG TGGTAAAAAC GATATAATCA TAGCTGTGAC GTATAACCGA 
GATGGGTCAT ATGACATGCA GATTGAAAAT AAGTTATTCC GAGTCCTGGG CGATCTTTCC 
AATGAGGATG GCTATACCTA CCTGAAGTCT TCTGTTAATG GAGTAGCCAG TAAATCCAAG 
TTCATCCTCC TGGACAACAC CATCTACCTG TTTTCTATGG AAGGCAGTAT TGAAGTTGGC 
ATTCCAGTAC CCAAGTACTT GTCTCCAGTG AGTGCAGAAG GAACTCAGGG AGGCACCATC 
GCTCCCATGA CTGGAACCAT TGAAAAGGTG TTCGTGAAGG CTGGAGACAG AGTAAAGGCT 
GGAGACGCTC TGATGGTTAT GATCGCCATG AAGATGGAGC ACACCATAAA AGCCCCAAAG 
GACGGCAGGA TAAAAAAGGT GTTTTTCAGT GAAGGAGCCC AAGCCAACAG ACATGCACCT 
TTAGTGGAGT TTGAAGAGGA GGAAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression, purification, characterization of human 3-methylcrotonyl-CoA carboxylase (MCCC).
Protein expression and purification53(2)421-7(2007 Jun)
Chu CH,Cheng D

Mitochondrial targeting signals and mature peptides of 3-methylcrotonyl-CoA carboxylase.
Biochemical and biophysical research communications334(3)939-46(2005 Sep)
Stadler SC,Polanetz R,Meier S,Mayerhofer PU,Herrmann JM,Anslinger K,Roscher AA,R?schinger W,Holzinger A

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

Proteomic analysis of the mouse liver mitochondrial inner membrane.
The Journal of biological chemistry278(42)41566-71(2003 Oct)
Da Cruz S,Xenarios I,Langridge J,Vilbois F,Parone PA,Martinou JC

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Mccc1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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