Mccc1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mccc1
Entrez Gene ID	43750
Full Name	CG2118 gene product from transcript CG2118-RC
Synonyms	Dmel\CG2118,MCC,Q9V9T5_DROME,mcc
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 100D2-100D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260462.2	NP_001247391.1	Methylcrotonoyl-CoA carboxylase 1, isoform C
NM_001260463.1	NP_001247392.1	Methylcrotonoyl-CoA carboxylase 1, isoform D
NM_143639.1	NP_651896.1	Methylcrotonoyl-CoA carboxylase 1, isoform A
NM_170577.3	NP_733456.1	Methylcrotonoyl-CoA carboxylase 1, isoform B

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mccc1	NP_001009653.1
Gallus gallus (chicken)	MCCC1	NP_001006551.1
Drosophila melanogaster (fruit fly)	CG2118	NP_001247391.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010228	XP_319416.4
Neurospora crassa	NCU00591	XP_965731.1
Oryza sativa (rice)	Os12g0605800	NP_001067226.1
Mus musculus (house mouse)	Mccc1	NP_076133.3
Danio rerio (zebrafish)	mccc1	NP_001038380.1
Magnaporthe oryzae (rice blast fungus)	MGG_10320	XP_003720502.1
Xenopus tropicalis (tropical clawed frog)	mccc1	NP_001006691.1
Homo sapiens (human)	MCCC1	NP_064551.3
	LOC708258	XP_001096717.1
Pan troglodytes (chimpanzee)	MCCC1	XP_516898.3
Canis lupus familiaris (dog)	MCCC1	XP_535817.2
Bos taurus (cattle)	MCCC1	NP_001192687.1
Caenorhabditis elegans (roundworm)	F32B6.2	NP_501777.2
Arabidopsis thaliana (thale cress)	MCCA	NP_849583.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mccc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mccc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06802	NM_001260463.1 Latest version!	Drosophila melanogaster Methylcrotonoyl-CoA carboxylase 1 (Mccc1), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa06802	NM_170577.3 Latest version!	Drosophila melanogaster Methylcrotonoyl-CoA carboxylase 1 (Mccc1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa06803	NM_143639.1 Latest version!	Drosophila melanogaster Methylcrotonoyl-CoA carboxylase 1 (Mccc1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa06803	NM_001260462.2 Latest version!	Drosophila melanogaster Methylcrotonoyl-CoA carboxylase 1 (Mccc1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mccc1 ( NM_001260463.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247392.1 Drosophila melanogaster Methylcrotonoyl-CoA carboxylase 1 (Mccc1), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa06802
Clone ID Related Accession (Same CDS sequence)	NM_001260463.1 , NM_170577.3
Accession Version	NM_001260463.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Methylcrotonoyl-CoA carboxylase 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACACGC AGTTGGCAGA CGAGTCGTAC CGCGTGGGGG AAGCTGCCTC CTCGGCTTCC 
TATCTTCGCG GTGAACGCAT CCTGGACATA GCCAAGCGTT CGGGCGCCCA GGCCATCCAC 
CCAGGCTACG GCTTCCTCTC CGAGTCCGTC GAGTTTGCCG AGTTGTGCCA GCGTGAGGGC 
ATCATCTTCA TGGGTCCGCC CAGTTCCGCC ATCCGGGACA TGGGGATTAA GAGCACCAGC 
AAGGCCATAA TGGCCGCTGC TGGGGTGCCG ATCATCAATG GATACCATGG CGATGATCAG 
TCAGATGAGT GTTTGCAGCG AGAAGCCGAC AAAATTGGCT TTCCCTTGAT GATTAAAGCC 
GTCCGCGGCG GCGGAGGCAA AGGCATGAGA ATCGCAGAAA AACCGGACGA CTTCCTCACC 
GCTCTGAACT CAGCTCGCAC CGAGTCCGAG AAGTCGTTTG GCGACAGCTC TGTGCTTCTG 
GAGCGGTACG TCCGATCTCC ACGCCACGTA GAGGTTCAAG TATTTGCGGA TCAGTATGGT 
GATGCTGTCT ATCTGTGGGA AAGAGATTGC TCCGTGCAAA GACGTCATCA AAAGATTATT 
GAAGAGGCGC CAGCTCCAGG TTTGTCGGAA GATTTGCGTC GTGAGCTGGG CGAGGCTGCT 
GTTCGTGCAG CGAAAGCTGT GGGCTACGTA GGAGCTGGCA CCGTGGAGTT CATTCTAGAC 
AAAGAAGATC TCTCTTTCCA CTTCATGGAA ATGAATACCC GCCTGCAGGT GGAGCATCCT 
ATAACGGAAA TGATTACGGG CACCGATTTG GTTGAATGGC AGATTCGCAT TGCCGCCGGT 
GAGCCATTAC CCCTGAAGCA ATCTGAGATT ACGCGGCGTG GCCACGCATT TGAAGCTCGC 
ATTTATGCAG AAAATCCTCG CGGAGGCTTT CTGCCCGGAG CCGGACCCCT GCGATATTTG 
TCTACTCCCC AACCATCAAA CAACGTTCGC GTGGAGACGG GTGTCCGAGA AGGAGATGAG 
GTGTCTGTGC ACTACGACCC CATGATTGCC AAGCTAGTCG TTTGGGGCGA AAACCGCACA 
CAAGCGCTTA ACTCATTGGT TGCCCGCCTG GGTGAATATC ATATATCTGG TCTGGACACT 
AACATTAATT TCCTCATCGA CCTTGCGTCG CATCCTGAGT TTCAGCTGGC CAATGTGCAC 
ACTGGCTTTA TCGATGAGCA GTTCGATACT CTATTTCCGC CAATTATCAT CAGTCCGCAG 
CAAGTCAGCC AAGCTGCTTT GGCGCTAGTC TTAAACGAGT TGCAGGCAGC CTTTAGGAAC 
GGAAACAAAG ATCAGGATCC ATTTGTTGCC ACCCCTAACG CTCGTCTGAA TTACTCTTTG 
GTTCGCAGAT ATGACTTGAA AGCCAATGAG AAGGTCTATT CTGTGGCTGT CAAGTTTGAT 
GGCGAGGATA TGCAAATTCA GGTGGATAAT GGCGACTGGC AGGATGCAAA GGTCGAAAGA 
GTTCAGGATG GAAGTCGTCT AAAAATTCGT GCCAATATCA ATAGCAACGT CACCACATAT 
AATGCTAGTA TTGATGGCAC TAGTGTCTCT TTGTTTTCGG AAAGTGGCAA GGTCGATTTT 
GAGGTGGGAC AGCCGAAATT CTTAAGTGCC CAGGGCGATC AATTGGGTGC AGTTGGGTCT 
CGCATAGTGG CTCCCATGCC CGGGATTTTA GAGAAGGTGC TGGTAAAGCC CGGTGACAAG 
GTCAAGAAGG GCGAAAACTT GGCAGTGCTT ATTGCCATGA AAATGGAACA CATTCTAAAA 
GCTCCAAAGG ACGCCACCAT TAAGTCTATT GGAGGAGCCG CGGGCGATAA CGTTGCTAAG 
GGAGCGGCCG TGATTACATT CGTCGACGAG GAAGTAGAAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247392.1
CDS	82..1986
Translation	MHTQLADESYRVGEAASSASYLRGERILDIAKRSGAQAIHPGYGFLSESVEFAELCQREGIIFMGPPSSAIRDMGIKSTSKAIMAAAGVPIINGYHGDDQSDECLQREADKIGFPLMIKAVRGGGGKGMRIAEKPDDFLTALNSARTESEKSFGDSSVLLERYVRSPRHVEVQVFADQYGDAVYLWERDCSVQRRHQKIIEEAPAPGLSEDLRRELGEAAVRAAKAVGYVGAGTVEFILDKEDLSFHFMEMNTRLQVEHPITEMITGTDLVEWQIRIAAGEPLPLKQSEITRRGHAFEARIYAENPRGGFLPGAGPLRYLSTPQPSNNVRVETGVREGDEVSVHYDPMIAKLVVWGENRTQALNSLVARLGEYHISGLDTNINFLIDLASHPEFQLANVHTGFIDEQFDTLFPPIIISPQQVSQAALALVLNELQAAFRNGNKDQDPFVATPNARLNYSLVRRYDLKANEKVYSVAVKFDGEDMQIQVDNGDWQDAKVERVQDGSRLKIRANINSNVTTYNASIDGTSVSLFSESGKVDFEVGQPKFLSAQGDQLGAVGSRIVAPMPGILEKVLVKPGDKVKKGENLAVLIAMKMEHILKAPKDATIKSIGGAAGDNVAKGAAVITFVDEEVEK

Target ORF information:

RefSeq Version	NM_001260463.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Methylcrotonoyl-CoA carboxylase 1 (Mccc1), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260463.1

ORF Insert Sequence:

ATGCACACGC AGTTGGCAGA CGAGTCGTAC CGCGTGGGGG AAGCTGCCTC CTCGGCTTCC 
TATCTTCGCG GTGAACGCAT CCTGGACATA GCCAAGCGTT CGGGCGCCCA GGCCATCCAC 
CCAGGCTACG GCTTCCTCTC CGAGTCCGTC GAGTTTGCCG AGTTGTGCCA GCGTGAGGGC 
ATCATCTTCA TGGGTCCGCC CAGTTCCGCC ATCCGGGACA TGGGGATTAA GAGCACCAGC 
AAGGCCATAA TGGCCGCTGC TGGGGTGCCG ATCATCAATG GATACCATGG CGATGATCAG 
TCAGATGAGT GTTTGCAGCG AGAAGCCGAC AAAATTGGCT TTCCCTTGAT GATTAAAGCC 
GTCCGCGGCG GCGGAGGCAA AGGCATGAGA ATCGCAGAAA AACCGGACGA CTTCCTCACC 
GCTCTGAACT CAGCTCGCAC CGAGTCCGAG AAGTCGTTTG GCGACAGCTC TGTGCTTCTG 
GAGCGGTACG TCCGATCTCC ACGCCACGTA GAGGTTCAAG TATTTGCGGA TCAGTATGGT 
GATGCTGTCT ATCTGTGGGA AAGAGATTGC TCCGTGCAAA GACGTCATCA AAAGATTATT 
GAAGAGGCGC CAGCTCCAGG TTTGTCGGAA GATTTGCGTC GTGAGCTGGG CGAGGCTGCT 
GTTCGTGCAG CGAAAGCTGT GGGCTACGTA GGAGCTGGCA CCGTGGAGTT CATTCTAGAC 
AAAGAAGATC TCTCTTTCCA CTTCATGGAA ATGAATACCC GCCTGCAGGT GGAGCATCCT 
ATAACGGAAA TGATTACGGG CACCGATTTG GTTGAATGGC AGATTCGCAT TGCCGCCGGT 
GAGCCATTAC CCCTGAAGCA ATCTGAGATT ACGCGGCGTG GCCACGCATT TGAAGCTCGC 
ATTTATGCAG AAAATCCTCG CGGAGGCTTT CTGCCCGGAG CCGGACCCCT GCGATATTTG 
TCTACTCCCC AACCATCAAA CAACGTTCGC GTGGAGACGG GTGTCCGAGA AGGAGATGAG 
GTGTCTGTGC ACTACGACCC CATGATTGCC AAGCTAGTCG TTTGGGGCGA AAACCGCACA 
CAAGCGCTTA ACTCATTGGT TGCCCGCCTG GGTGAATATC ATATATCTGG TCTGGACACT 
AACATTAATT TCCTCATCGA CCTTGCGTCG CATCCTGAGT TTCAGCTGGC CAATGTGCAC 
ACTGGCTTTA TCGATGAGCA GTTCGATACT CTATTTCCGC CAATTATCAT CAGTCCGCAG 
CAAGTCAGCC AAGCTGCTTT GGCGCTAGTC TTAAACGAGT TGCAGGCAGC CTTTAGGAAC 
GGAAACAAAG ATCAGGATCC ATTTGTTGCC ACCCCTAACG CTCGTCTGAA TTACTCTTTG 
GTTCGCAGAT ATGACTTGAA AGCCAATGAG AAGGTCTATT CTGTGGCTGT CAAGTTTGAT 
GGCGAGGATA TGCAAATTCA GGTGGATAAT GGCGACTGGC AGGATGCAAA GGTCGAAAGA 
GTTCAGGATG GAAGTCGTCT AAAAATTCGT GCCAATATCA ATAGCAACGT CACCACATAT 
AATGCTAGTA TTGATGGCAC TAGTGTCTCT TTGTTTTCGG AAAGTGGCAA GGTCGATTTT 
GAGGTGGGAC AGCCGAAATT CTTAAGTGCC CAGGGCGATC AATTGGGTGC AGTTGGGTCT 
CGCATAGTGG CTCCCATGCC CGGGATTTTA GAGAAGGTGC TGGTAAAGCC CGGTGACAAG 
GTCAAGAAGG GCGAAAACTT GGCAGTGCTT ATTGCCATGA AAATGGAACA CATTCTAAAA 
GCTCCAAAGG ACGCCACCAT TAAGTCTATT GGAGGAGCCG CGGGCGATAA CGTTGCTAAG 
GGAGCGGCCG TGATTACATT CGTCGACGAG GAAGTAGAAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mccc1 ( NM_170577.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733456.1 Drosophila melanogaster Methylcrotonoyl-CoA carboxylase 1 (Mccc1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa06802
Clone ID Related Accession (Same CDS sequence)	NM_001260463.1 , NM_170577.3
Accession Version	NM_170577.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Methylcrotonoyl-CoA carboxylase 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_170577.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACACGC AGTTGGCAGA CGAGTCGTAC CGCGTGGGGG AAGCTGCCTC CTCGGCTTCC 
TATCTTCGCG GTGAACGCAT CCTGGACATA GCCAAGCGTT CGGGCGCCCA GGCCATCCAC 
CCAGGCTACG GCTTCCTCTC CGAGTCCGTC GAGTTTGCCG AGTTGTGCCA GCGTGAGGGC 
ATCATCTTCA TGGGTCCGCC CAGTTCCGCC ATCCGGGACA TGGGGATTAA GAGCACCAGC 
AAGGCCATAA TGGCCGCTGC TGGGGTGCCG ATCATCAATG GATACCATGG CGATGATCAG 
TCAGATGAGT GTTTGCAGCG AGAAGCCGAC AAAATTGGCT TTCCCTTGAT GATTAAAGCC 
GTCCGCGGCG GCGGAGGCAA AGGCATGAGA ATCGCAGAAA AACCGGACGA CTTCCTCACC 
GCTCTGAACT CAGCTCGCAC CGAGTCCGAG AAGTCGTTTG GCGACAGCTC TGTGCTTCTG 
GAGCGGTACG TCCGATCTCC ACGCCACGTA GAGGTTCAAG TATTTGCGGA TCAGTATGGT 
GATGCTGTCT ATCTGTGGGA AAGAGATTGC TCCGTGCAAA GACGTCATCA AAAGATTATT 
GAAGAGGCGC CAGCTCCAGG TTTGTCGGAA GATTTGCGTC GTGAGCTGGG CGAGGCTGCT 
GTTCGTGCAG CGAAAGCTGT GGGCTACGTA GGAGCTGGCA CCGTGGAGTT CATTCTAGAC 
AAAGAAGATC TCTCTTTCCA CTTCATGGAA ATGAATACCC GCCTGCAGGT GGAGCATCCT 
ATAACGGAAA TGATTACGGG CACCGATTTG GTTGAATGGC AGATTCGCAT TGCCGCCGGT 
GAGCCATTAC CCCTGAAGCA ATCTGAGATT ACGCGGCGTG GCCACGCATT TGAAGCTCGC 
ATTTATGCAG AAAATCCTCG CGGAGGCTTT CTGCCCGGAG CCGGACCCCT GCGATATTTG 
TCTACTCCCC AACCATCAAA CAACGTTCGC GTGGAGACGG GTGTCCGAGA AGGAGATGAG 
GTGTCTGTGC ACTACGACCC CATGATTGCC AAGCTAGTCG TTTGGGGCGA AAACCGCACA 
CAAGCGCTTA ACTCATTGGT TGCCCGCCTG GGTGAATATC ATATATCTGG TCTGGACACT 
AACATTAATT TCCTCATCGA CCTTGCGTCG CATCCTGAGT TTCAGCTGGC CAATGTGCAC 
ACTGGCTTTA TCGATGAGCA GTTCGATACT CTATTTCCGC CAATTATCAT CAGTCCGCAG 
CAAGTCAGCC AAGCTGCTTT GGCGCTAGTC TTAAACGAGT TGCAGGCAGC CTTTAGGAAC 
GGAAACAAAG ATCAGGATCC ATTTGTTGCC ACCCCTAACG CTCGTCTGAA TTACTCTTTG 
GTTCGCAGAT ATGACTTGAA AGCCAATGAG AAGGTCTATT CTGTGGCTGT CAAGTTTGAT 
GGCGAGGATA TGCAAATTCA GGTGGATAAT GGCGACTGGC AGGATGCAAA GGTCGAAAGA 
GTTCAGGATG GAAGTCGTCT AAAAATTCGT GCCAATATCA ATAGCAACGT CACCACATAT 
AATGCTAGTA TTGATGGCAC TAGTGTCTCT TTGTTTTCGG AAAGTGGCAA GGTCGATTTT 
GAGGTGGGAC AGCCGAAATT CTTAAGTGCC CAGGGCGATC AATTGGGTGC AGTTGGGTCT 
CGCATAGTGG CTCCCATGCC CGGGATTTTA GAGAAGGTGC TGGTAAAGCC CGGTGACAAG 
GTCAAGAAGG GCGAAAACTT GGCAGTGCTT ATTGCCATGA AAATGGAACA CATTCTAAAA 
GCTCCAAAGG ACGCCACCAT TAAGTCTATT GGAGGAGCCG CGGGCGATAA CGTTGCTAAG 
GGAGCGGCCG TGATTACATT CGTCGACGAG GAAGTAGAAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733456.1
CDS	213..2117
Translation	MHTQLADESYRVGEAASSASYLRGERILDIAKRSGAQAIHPGYGFLSESVEFAELCQREGIIFMGPPSSAIRDMGIKSTSKAIMAAAGVPIINGYHGDDQSDECLQREADKIGFPLMIKAVRGGGGKGMRIAEKPDDFLTALNSARTESEKSFGDSSVLLERYVRSPRHVEVQVFADQYGDAVYLWERDCSVQRRHQKIIEEAPAPGLSEDLRRELGEAAVRAAKAVGYVGAGTVEFILDKEDLSFHFMEMNTRLQVEHPITEMITGTDLVEWQIRIAAGEPLPLKQSEITRRGHAFEARIYAENPRGGFLPGAGPLRYLSTPQPSNNVRVETGVREGDEVSVHYDPMIAKLVVWGENRTQALNSLVARLGEYHISGLDTNINFLIDLASHPEFQLANVHTGFIDEQFDTLFPPIIISPQQVSQAALALVLNELQAAFRNGNKDQDPFVATPNARLNYSLVRRYDLKANEKVYSVAVKFDGEDMQIQVDNGDWQDAKVERVQDGSRLKIRANINSNVTTYNASIDGTSVSLFSESGKVDFEVGQPKFLSAQGDQLGAVGSRIVAPMPGILEKVLVKPGDKVKKGENLAVLIAMKMEHILKAPKDATIKSIGGAAGDNVAKGAAVITFVDEEVEK

Target ORF information:

RefSeq Version	NM_170577.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Methylcrotonoyl-CoA carboxylase 1 (Mccc1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170577.3

ORF Insert Sequence:

ATGCACACGC AGTTGGCAGA CGAGTCGTAC CGCGTGGGGG AAGCTGCCTC CTCGGCTTCC 
TATCTTCGCG GTGAACGCAT CCTGGACATA GCCAAGCGTT CGGGCGCCCA GGCCATCCAC 
CCAGGCTACG GCTTCCTCTC CGAGTCCGTC GAGTTTGCCG AGTTGTGCCA GCGTGAGGGC 
ATCATCTTCA TGGGTCCGCC CAGTTCCGCC ATCCGGGACA TGGGGATTAA GAGCACCAGC 
AAGGCCATAA TGGCCGCTGC TGGGGTGCCG ATCATCAATG GATACCATGG CGATGATCAG 
TCAGATGAGT GTTTGCAGCG AGAAGCCGAC AAAATTGGCT TTCCCTTGAT GATTAAAGCC 
GTCCGCGGCG GCGGAGGCAA AGGCATGAGA ATCGCAGAAA AACCGGACGA CTTCCTCACC 
GCTCTGAACT CAGCTCGCAC CGAGTCCGAG AAGTCGTTTG GCGACAGCTC TGTGCTTCTG 
GAGCGGTACG TCCGATCTCC ACGCCACGTA GAGGTTCAAG TATTTGCGGA TCAGTATGGT 
GATGCTGTCT ATCTGTGGGA AAGAGATTGC TCCGTGCAAA GACGTCATCA AAAGATTATT 
GAAGAGGCGC CAGCTCCAGG TTTGTCGGAA GATTTGCGTC GTGAGCTGGG CGAGGCTGCT 
GTTCGTGCAG CGAAAGCTGT GGGCTACGTA GGAGCTGGCA CCGTGGAGTT CATTCTAGAC 
AAAGAAGATC TCTCTTTCCA CTTCATGGAA ATGAATACCC GCCTGCAGGT GGAGCATCCT 
ATAACGGAAA TGATTACGGG CACCGATTTG GTTGAATGGC AGATTCGCAT TGCCGCCGGT 
GAGCCATTAC CCCTGAAGCA ATCTGAGATT ACGCGGCGTG GCCACGCATT TGAAGCTCGC 
ATTTATGCAG AAAATCCTCG CGGAGGCTTT CTGCCCGGAG CCGGACCCCT GCGATATTTG 
TCTACTCCCC AACCATCAAA CAACGTTCGC GTGGAGACGG GTGTCCGAGA AGGAGATGAG 
GTGTCTGTGC ACTACGACCC CATGATTGCC AAGCTAGTCG TTTGGGGCGA AAACCGCACA 
CAAGCGCTTA ACTCATTGGT TGCCCGCCTG GGTGAATATC ATATATCTGG TCTGGACACT 
AACATTAATT TCCTCATCGA CCTTGCGTCG CATCCTGAGT TTCAGCTGGC CAATGTGCAC 
ACTGGCTTTA TCGATGAGCA GTTCGATACT CTATTTCCGC CAATTATCAT CAGTCCGCAG 
CAAGTCAGCC AAGCTGCTTT GGCGCTAGTC TTAAACGAGT TGCAGGCAGC CTTTAGGAAC 
GGAAACAAAG ATCAGGATCC ATTTGTTGCC ACCCCTAACG CTCGTCTGAA TTACTCTTTG 
GTTCGCAGAT ATGACTTGAA AGCCAATGAG AAGGTCTATT CTGTGGCTGT CAAGTTTGAT 
GGCGAGGATA TGCAAATTCA GGTGGATAAT GGCGACTGGC AGGATGCAAA GGTCGAAAGA 
GTTCAGGATG GAAGTCGTCT AAAAATTCGT GCCAATATCA ATAGCAACGT CACCACATAT 
AATGCTAGTA TTGATGGCAC TAGTGTCTCT TTGTTTTCGG AAAGTGGCAA GGTCGATTTT 
GAGGTGGGAC AGCCGAAATT CTTAAGTGCC CAGGGCGATC AATTGGGTGC AGTTGGGTCT 
CGCATAGTGG CTCCCATGCC CGGGATTTTA GAGAAGGTGC TGGTAAAGCC CGGTGACAAG 
GTCAAGAAGG GCGAAAACTT GGCAGTGCTT ATTGCCATGA AAATGGAACA CATTCTAAAA 
GCTCCAAAGG ACGCCACCAT TAAGTCTATT GGAGGAGCCG CGGGCGATAA CGTTGCTAAG 
GGAGCGGCCG TGATTACATT CGTCGACGAG GAAGTAGAAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mccc1 ( NM_001260462.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247391.1 Drosophila melanogaster Methylcrotonoyl-CoA carboxylase 1 (Mccc1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa06803
Clone ID Related Accession (Same CDS sequence)	NM_001260462.2 , NM_143639.1
Accession Version	NM_001260462.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Methylcrotonoyl-CoA carboxylase 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001260462.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACGCCA GACTGCTGCG AAACTTCGGC GCAAACGCCA GGTGCTTTGC AACAGAAGCC 
AGCCCAAAAG TTCGGCCTAT CAGCAAGATC CTTATTTCAA ATCGTGGTGA GATCGCATGC 
AGGGTGATTC GAACCGCACG CAAGCTGGGA GTTCGTACCG TGGCCGTGTT CTCGGATCCG 
GACGAGAAGA GCATGCACAC GCAGTTGGCA GACGAGTCGT ACCGCGTGGG GGAAGCTGCC 
TCCTCGGCTT CCTATCTTCG CGGTGAACGC ATCCTGGACA TAGCCAAGCG TTCGGGCGCC 
CAGGCCATCC ACCCAGGCTA CGGCTTCCTC TCCGAGTCCG TCGAGTTTGC CGAGTTGTGC 
CAGCGTGAGG GCATCATCTT CATGGGTCCG CCCAGTTCCG CCATCCGGGA CATGGGGATT 
AAGAGCACCA GCAAGGCCAT AATGGCCGCT GCTGGGGTGC CGATCATCAA TGGATACCAT 
GGCGATGATC AGTCAGATGA GTGTTTGCAG CGAGAAGCCG ACAAAATTGG CTTTCCCTTG 
ATGATTAAAG CCGTCCGCGG CGGCGGAGGC AAAGGCATGA GAATCGCAGA AAAACCGGAC 
GACTTCCTCA CCGCTCTGAA CTCAGCTCGC ACCGAGTCCG AGAAGTCGTT TGGCGACAGC 
TCTGTGCTTC TGGAGCGGTA CGTCCGATCT CCACGCCACG TAGAGGTTCA AGTATTTGCG 
GATCAGTATG GTGATGCTGT CTATCTGTGG GAAAGAGATT GCTCCGTGCA AAGACGTCAT 
CAAAAGATTA TTGAAGAGGC GCCAGCTCCA GGTTTGTCGG AAGATTTGCG TCGTGAGCTG 
GGCGAGGCTG CTGTTCGTGC AGCGAAAGCT GTGGGCTACG TAGGAGCTGG CACCGTGGAG 
TTCATTCTAG ACAAAGAAGA TCTCTCTTTC CACTTCATGG AAATGAATAC CCGCCTGCAG 
GTGGAGCATC CTATAACGGA AATGATTACG GGCACCGATT TGGTTGAATG GCAGATTCGC 
ATTGCCGCCG GTGAGCCATT ACCCCTGAAG CAATCTGAGA TTACGCGGCG TGGCCACGCA 
TTTGAAGCTC GCATTTATGC AGAAAATCCT CGCGGAGGCT TTCTGCCCGG AGCCGGACCC 
CTGCGATATT TGTCTACTCC CCAACCATCA AACAACGTTC GCGTGGAGAC GGGTGTCCGA 
GAAGGAGATG AGGTGTCTGT GCACTACGAC CCCATGATTG CCAAGCTAGT CGTTTGGGGC 
GAAAACCGCA CACAAGCGCT TAACTCATTG GTTGCCCGCC TGGGTGAATA TCATATATCT 
GGTCTGGACA CTAACATTAA TTTCCTCATC GACCTTGCGT CGCATCCTGA GTTTCAGCTG 
GCCAATGTGC ACACTGGCTT TATCGATGAG CAGTTCGATA CTCTATTTCC GCCAATTATC 
ATCAGTCCGC AGCAAGTCAG CCAAGCTGCT TTGGCGCTAG TCTTAAACGA GTTGCAGGCA 
GCCTTTAGGA ACGGAAACAA AGATCAGGAT CCATTTGTTG CCACCCCTAA CGCTCGTCTG 
AATTACTCTT TGGTTCGCAG ATATGACTTG AAAGCCAATG AGAAGGTCTA TTCTGTGGCT 
GTCAAGTTTG ATGGCGAGGA TATGCAAATT CAGGTGGATA ATGGCGACTG GCAGGATGCA 
AAGGTCGAAA GAGTTCAGGA TGGAAGTCGT CTAAAAATTC GTGCCAATAT CAATAGCAAC 
GTCACCACAT ATAATGCTAG TATTGATGGC ACTAGTGTCT CTTTGTTTTC GGAAAGTGGC 
AAGGTCGATT TTGAGGTGGG ACAGCCGAAA TTCTTAAGTG CCCAGGGCGA TCAATTGGGT 
GCAGTTGGGT CTCGCATAGT GGCTCCCATG CCCGGGATTT TAGAGAAGGT GCTGGTAAAG 
CCCGGTGACA AGGTCAAGAA GGGCGAAAAC TTGGCAGTGC TTATTGCCAT GAAAATGGAA 
CACATTCTAA AAGCTCCAAA GGACGCCACC ATTAAGTCTA TTGGAGGAGC CGCGGGCGAT 
AACGTTGCTA AGGGAGCGGC CGTGATTACA TTCGTCGACG AGGAAGTAGA AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247391.1
CDS	290..2386
Translation	MYARLLRNFGANARCFATEASPKVRPISKILISNRGEIACRVIRTARKLGVRTVAVFSDPDEKSMHTQLADESYRVGEAASSASYLRGERILDIAKRSGAQAIHPGYGFLSESVEFAELCQREGIIFMGPPSSAIRDMGIKSTSKAIMAAAGVPIINGYHGDDQSDECLQREADKIGFPLMIKAVRGGGGKGMRIAEKPDDFLTALNSARTESEKSFGDSSVLLERYVRSPRHVEVQVFADQYGDAVYLWERDCSVQRRHQKIIEEAPAPGLSEDLRRELGEAAVRAAKAVGYVGAGTVEFILDKEDLSFHFMEMNTRLQVEHPITEMITGTDLVEWQIRIAAGEPLPLKQSEITRRGHAFEARIYAENPRGGFLPGAGPLRYLSTPQPSNNVRVETGVREGDEVSVHYDPMIAKLVVWGENRTQALNSLVARLGEYHISGLDTNINFLIDLASHPEFQLANVHTGFIDEQFDTLFPPIIISPQQVSQAALALVLNELQAAFRNGNKDQDPFVATPNARLNYSLVRRYDLKANEKVYSVAVKFDGEDMQIQVDNGDWQDAKVERVQDGSRLKIRANINSNVTTYNASIDGTSVSLFSESGKVDFEVGQPKFLSAQGDQLGAVGSRIVAPMPGILEKVLVKPGDKVKKGENLAVLIAMKMEHILKAPKDATIKSIGGAAGDNVAKGAAVITFVDEEVEK

Target ORF information:

RefSeq Version	NM_001260462.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Methylcrotonoyl-CoA carboxylase 1 (Mccc1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260462.2

ORF Insert Sequence:

ATGTACGCCA GACTGCTGCG AAACTTCGGC GCAAACGCCA GGTGCTTTGC AACAGAAGCC 
AGCCCAAAAG TTCGGCCTAT CAGCAAGATC CTTATTTCAA ATCGTGGTGA GATCGCATGC 
AGGGTGATTC GAACCGCACG CAAGCTGGGA GTTCGTACCG TGGCCGTGTT CTCGGATCCG 
GACGAGAAGA GCATGCACAC GCAGTTGGCA GACGAGTCGT ACCGCGTGGG GGAAGCTGCC 
TCCTCGGCTT CCTATCTTCG CGGTGAACGC ATCCTGGACA TAGCCAAGCG TTCGGGCGCC 
CAGGCCATCC ACCCAGGCTA CGGCTTCCTC TCCGAGTCCG TCGAGTTTGC CGAGTTGTGC 
CAGCGTGAGG GCATCATCTT CATGGGTCCG CCCAGTTCCG CCATCCGGGA CATGGGGATT 
AAGAGCACCA GCAAGGCCAT AATGGCCGCT GCTGGGGTGC CGATCATCAA TGGATACCAT 
GGCGATGATC AGTCAGATGA GTGTTTGCAG CGAGAAGCCG ACAAAATTGG CTTTCCCTTG 
ATGATTAAAG CCGTCCGCGG CGGCGGAGGC AAAGGCATGA GAATCGCAGA AAAACCGGAC 
GACTTCCTCA CCGCTCTGAA CTCAGCTCGC ACCGAGTCCG AGAAGTCGTT TGGCGACAGC 
TCTGTGCTTC TGGAGCGGTA CGTCCGATCT CCACGCCACG TAGAGGTTCA AGTATTTGCG 
GATCAGTATG GTGATGCTGT CTATCTGTGG GAAAGAGATT GCTCCGTGCA AAGACGTCAT 
CAAAAGATTA TTGAAGAGGC GCCAGCTCCA GGTTTGTCGG AAGATTTGCG TCGTGAGCTG 
GGCGAGGCTG CTGTTCGTGC AGCGAAAGCT GTGGGCTACG TAGGAGCTGG CACCGTGGAG 
TTCATTCTAG ACAAAGAAGA TCTCTCTTTC CACTTCATGG AAATGAATAC CCGCCTGCAG 
GTGGAGCATC CTATAACGGA AATGATTACG GGCACCGATT TGGTTGAATG GCAGATTCGC 
ATTGCCGCCG GTGAGCCATT ACCCCTGAAG CAATCTGAGA TTACGCGGCG TGGCCACGCA 
TTTGAAGCTC GCATTTATGC AGAAAATCCT CGCGGAGGCT TTCTGCCCGG AGCCGGACCC 
CTGCGATATT TGTCTACTCC CCAACCATCA AACAACGTTC GCGTGGAGAC GGGTGTCCGA 
GAAGGAGATG AGGTGTCTGT GCACTACGAC CCCATGATTG CCAAGCTAGT CGTTTGGGGC 
GAAAACCGCA CACAAGCGCT TAACTCATTG GTTGCCCGCC TGGGTGAATA TCATATATCT 
GGTCTGGACA CTAACATTAA TTTCCTCATC GACCTTGCGT CGCATCCTGA GTTTCAGCTG 
GCCAATGTGC ACACTGGCTT TATCGATGAG CAGTTCGATA CTCTATTTCC GCCAATTATC 
ATCAGTCCGC AGCAAGTCAG CCAAGCTGCT TTGGCGCTAG TCTTAAACGA GTTGCAGGCA 
GCCTTTAGGA ACGGAAACAA AGATCAGGAT CCATTTGTTG CCACCCCTAA CGCTCGTCTG 
AATTACTCTT TGGTTCGCAG ATATGACTTG AAAGCCAATG AGAAGGTCTA TTCTGTGGCT 
GTCAAGTTTG ATGGCGAGGA TATGCAAATT CAGGTGGATA ATGGCGACTG GCAGGATGCA 
AAGGTCGAAA GAGTTCAGGA TGGAAGTCGT CTAAAAATTC GTGCCAATAT CAATAGCAAC 
GTCACCACAT ATAATGCTAG TATTGATGGC ACTAGTGTCT CTTTGTTTTC GGAAAGTGGC 
AAGGTCGATT TTGAGGTGGG ACAGCCGAAA TTCTTAAGTG CCCAGGGCGA TCAATTGGGT 
GCAGTTGGGT CTCGCATAGT GGCTCCCATG CCCGGGATTT TAGAGAAGGT GCTGGTAAAG 
CCCGGTGACA AGGTCAAGAA GGGCGAAAAC TTGGCAGTGC TTATTGCCAT GAAAATGGAA 
CACATTCTAA AAGCTCCAAA GGACGCCACC ATTAAGTCTA TTGGAGGAGC CGCGGGCGAT 
AACGTTGCTA AGGGAGCGGC CGTGATTACA TTCGTCGACG AGGAAGTAGA AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mccc1 ( NM_143639.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651896.1 Drosophila melanogaster Methylcrotonoyl-CoA carboxylase 1 (Mccc1), transcript variant A, mRNA.

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CloneID	OFa06803
Clone ID Related Accession (Same CDS sequence)	NM_001260462.2 , NM_143639.1
Accession Version	NM_143639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Methylcrotonoyl-CoA carboxylase 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACGCCA GACTGCTGCG AAACTTCGGC GCAAACGCCA GGTGCTTTGC AACAGAAGCC 
AGCCCAAAAG TTCGGCCTAT CAGCAAGATC CTTATTTCAA ATCGTGGTGA GATCGCATGC 
AGGGTGATTC GAACCGCACG CAAGCTGGGA GTTCGTACCG TGGCCGTGTT CTCGGATCCG 
GACGAGAAGA GCATGCACAC GCAGTTGGCA GACGAGTCGT ACCGCGTGGG GGAAGCTGCC 
TCCTCGGCTT CCTATCTTCG CGGTGAACGC ATCCTGGACA TAGCCAAGCG TTCGGGCGCC 
CAGGCCATCC ACCCAGGCTA CGGCTTCCTC TCCGAGTCCG TCGAGTTTGC CGAGTTGTGC 
CAGCGTGAGG GCATCATCTT CATGGGTCCG CCCAGTTCCG CCATCCGGGA CATGGGGATT 
AAGAGCACCA GCAAGGCCAT AATGGCCGCT GCTGGGGTGC CGATCATCAA TGGATACCAT 
GGCGATGATC AGTCAGATGA GTGTTTGCAG CGAGAAGCCG ACAAAATTGG CTTTCCCTTG 
ATGATTAAAG CCGTCCGCGG CGGCGGAGGC AAAGGCATGA GAATCGCAGA AAAACCGGAC 
GACTTCCTCA CCGCTCTGAA CTCAGCTCGC ACCGAGTCCG AGAAGTCGTT TGGCGACAGC 
TCTGTGCTTC TGGAGCGGTA CGTCCGATCT CCACGCCACG TAGAGGTTCA AGTATTTGCG 
GATCAGTATG GTGATGCTGT CTATCTGTGG GAAAGAGATT GCTCCGTGCA AAGACGTCAT 
CAAAAGATTA TTGAAGAGGC GCCAGCTCCA GGTTTGTCGG AAGATTTGCG TCGTGAGCTG 
GGCGAGGCTG CTGTTCGTGC AGCGAAAGCT GTGGGCTACG TAGGAGCTGG CACCGTGGAG 
TTCATTCTAG ACAAAGAAGA TCTCTCTTTC CACTTCATGG AAATGAATAC CCGCCTGCAG 
GTGGAGCATC CTATAACGGA AATGATTACG GGCACCGATT TGGTTGAATG GCAGATTCGC 
ATTGCCGCCG GTGAGCCATT ACCCCTGAAG CAATCTGAGA TTACGCGGCG TGGCCACGCA 
TTTGAAGCTC GCATTTATGC AGAAAATCCT CGCGGAGGCT TTCTGCCCGG AGCCGGACCC 
CTGCGATATT TGTCTACTCC CCAACCATCA AACAACGTTC GCGTGGAGAC GGGTGTCCGA 
GAAGGAGATG AGGTGTCTGT GCACTACGAC CCCATGATTG CCAAGCTAGT CGTTTGGGGC 
GAAAACCGCA CACAAGCGCT TAACTCATTG GTTGCCCGCC TGGGTGAATA TCATATATCT 
GGTCTGGACA CTAACATTAA TTTCCTCATC GACCTTGCGT CGCATCCTGA GTTTCAGCTG 
GCCAATGTGC ACACTGGCTT TATCGATGAG CAGTTCGATA CTCTATTTCC GCCAATTATC 
ATCAGTCCGC AGCAAGTCAG CCAAGCTGCT TTGGCGCTAG TCTTAAACGA GTTGCAGGCA 
GCCTTTAGGA ACGGAAACAA AGATCAGGAT CCATTTGTTG CCACCCCTAA CGCTCGTCTG 
AATTACTCTT TGGTTCGCAG ATATGACTTG AAAGCCAATG AGAAGGTCTA TTCTGTGGCT 
GTCAAGTTTG ATGGCGAGGA TATGCAAATT CAGGTGGATA ATGGCGACTG GCAGGATGCA 
AAGGTCGAAA GAGTTCAGGA TGGAAGTCGT CTAAAAATTC GTGCCAATAT CAATAGCAAC 
GTCACCACAT ATAATGCTAG TATTGATGGC ACTAGTGTCT CTTTGTTTTC GGAAAGTGGC 
AAGGTCGATT TTGAGGTGGG ACAGCCGAAA TTCTTAAGTG CCCAGGGCGA TCAATTGGGT 
GCAGTTGGGT CTCGCATAGT GGCTCCCATG CCCGGGATTT TAGAGAAGGT GCTGGTAAAG 
CCCGGTGACA AGGTCAAGAA GGGCGAAAAC TTGGCAGTGC TTATTGCCAT GAAAATGGAA 
CACATTCTAA AAGCTCCAAA GGACGCCACC ATTAAGTCTA TTGGAGGAGC CGCGGGCGAT 
AACGTTGCTA AGGGAGCGGC CGTGATTACA TTCGTCGACG AGGAAGTAGA AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651896.1
CDS	111..2207
Translation	MYARLLRNFGANARCFATEASPKVRPISKILISNRGEIACRVIRTARKLGVRTVAVFSDPDEKSMHTQLADESYRVGEAASSASYLRGERILDIAKRSGAQAIHPGYGFLSESVEFAELCQREGIIFMGPPSSAIRDMGIKSTSKAIMAAAGVPIINGYHGDDQSDECLQREADKIGFPLMIKAVRGGGGKGMRIAEKPDDFLTALNSARTESEKSFGDSSVLLERYVRSPRHVEVQVFADQYGDAVYLWERDCSVQRRHQKIIEEAPAPGLSEDLRRELGEAAVRAAKAVGYVGAGTVEFILDKEDLSFHFMEMNTRLQVEHPITEMITGTDLVEWQIRIAAGEPLPLKQSEITRRGHAFEARIYAENPRGGFLPGAGPLRYLSTPQPSNNVRVETGVREGDEVSVHYDPMIAKLVVWGENRTQALNSLVARLGEYHISGLDTNINFLIDLASHPEFQLANVHTGFIDEQFDTLFPPIIISPQQVSQAALALVLNELQAAFRNGNKDQDPFVATPNARLNYSLVRRYDLKANEKVYSVAVKFDGEDMQIQVDNGDWQDAKVERVQDGSRLKIRANINSNVTTYNASIDGTSVSLFSESGKVDFEVGQPKFLSAQGDQLGAVGSRIVAPMPGILEKVLVKPGDKVKKGENLAVLIAMKMEHILKAPKDATIKSIGGAAGDNVAKGAAVITFVDEEVEK

Target ORF information:

RefSeq Version	NM_143639.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Methylcrotonoyl-CoA carboxylase 1 (Mccc1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143639.1

ORF Insert Sequence:

ATGTACGCCA GACTGCTGCG AAACTTCGGC GCAAACGCCA GGTGCTTTGC AACAGAAGCC 
AGCCCAAAAG TTCGGCCTAT CAGCAAGATC CTTATTTCAA ATCGTGGTGA GATCGCATGC 
AGGGTGATTC GAACCGCACG CAAGCTGGGA GTTCGTACCG TGGCCGTGTT CTCGGATCCG 
GACGAGAAGA GCATGCACAC GCAGTTGGCA GACGAGTCGT ACCGCGTGGG GGAAGCTGCC 
TCCTCGGCTT CCTATCTTCG CGGTGAACGC ATCCTGGACA TAGCCAAGCG TTCGGGCGCC 
CAGGCCATCC ACCCAGGCTA CGGCTTCCTC TCCGAGTCCG TCGAGTTTGC CGAGTTGTGC 
CAGCGTGAGG GCATCATCTT CATGGGTCCG CCCAGTTCCG CCATCCGGGA CATGGGGATT 
AAGAGCACCA GCAAGGCCAT AATGGCCGCT GCTGGGGTGC CGATCATCAA TGGATACCAT 
GGCGATGATC AGTCAGATGA GTGTTTGCAG CGAGAAGCCG ACAAAATTGG CTTTCCCTTG 
ATGATTAAAG CCGTCCGCGG CGGCGGAGGC AAAGGCATGA GAATCGCAGA AAAACCGGAC 
GACTTCCTCA CCGCTCTGAA CTCAGCTCGC ACCGAGTCCG AGAAGTCGTT TGGCGACAGC 
TCTGTGCTTC TGGAGCGGTA CGTCCGATCT CCACGCCACG TAGAGGTTCA AGTATTTGCG 
GATCAGTATG GTGATGCTGT CTATCTGTGG GAAAGAGATT GCTCCGTGCA AAGACGTCAT 
CAAAAGATTA TTGAAGAGGC GCCAGCTCCA GGTTTGTCGG AAGATTTGCG TCGTGAGCTG 
GGCGAGGCTG CTGTTCGTGC AGCGAAAGCT GTGGGCTACG TAGGAGCTGG CACCGTGGAG 
TTCATTCTAG ACAAAGAAGA TCTCTCTTTC CACTTCATGG AAATGAATAC CCGCCTGCAG 
GTGGAGCATC CTATAACGGA AATGATTACG GGCACCGATT TGGTTGAATG GCAGATTCGC 
ATTGCCGCCG GTGAGCCATT ACCCCTGAAG CAATCTGAGA TTACGCGGCG TGGCCACGCA 
TTTGAAGCTC GCATTTATGC AGAAAATCCT CGCGGAGGCT TTCTGCCCGG AGCCGGACCC 
CTGCGATATT TGTCTACTCC CCAACCATCA AACAACGTTC GCGTGGAGAC GGGTGTCCGA 
GAAGGAGATG AGGTGTCTGT GCACTACGAC CCCATGATTG CCAAGCTAGT CGTTTGGGGC 
GAAAACCGCA CACAAGCGCT TAACTCATTG GTTGCCCGCC TGGGTGAATA TCATATATCT 
GGTCTGGACA CTAACATTAA TTTCCTCATC GACCTTGCGT CGCATCCTGA GTTTCAGCTG 
GCCAATGTGC ACACTGGCTT TATCGATGAG CAGTTCGATA CTCTATTTCC GCCAATTATC 
ATCAGTCCGC AGCAAGTCAG CCAAGCTGCT TTGGCGCTAG TCTTAAACGA GTTGCAGGCA 
GCCTTTAGGA ACGGAAACAA AGATCAGGAT CCATTTGTTG CCACCCCTAA CGCTCGTCTG 
AATTACTCTT TGGTTCGCAG ATATGACTTG AAAGCCAATG AGAAGGTCTA TTCTGTGGCT 
GTCAAGTTTG ATGGCGAGGA TATGCAAATT CAGGTGGATA ATGGCGACTG GCAGGATGCA 
AAGGTCGAAA GAGTTCAGGA TGGAAGTCGT CTAAAAATTC GTGCCAATAT CAATAGCAAC 
GTCACCACAT ATAATGCTAG TATTGATGGC ACTAGTGTCT CTTTGTTTTC GGAAAGTGGC 
AAGGTCGATT TTGAGGTGGG ACAGCCGAAA TTCTTAAGTG CCCAGGGCGA TCAATTGGGT 
GCAGTTGGGT CTCGCATAGT GGCTCCCATG CCCGGGATTT TAGAGAAGGT GCTGGTAAAG 
CCCGGTGACA AGGTCAAGAA GGGCGAAAAC TTGGCAGTGC TTATTGCCAT GAAAATGGAA 
CACATTCTAA AAGCTCCAAA GGACGCCACC ATTAAGTCTA TTGGAGGAGC CGCGGGCGAT 
AACGTTGCTA AGGGAGCGGC CGTGATTACA TTCGTCGACG AGGAAGTAGA AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mccc1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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